CN103655809A - Natural composition and preparation method thereof - Google Patents
Natural composition and preparation method thereof Download PDFInfo
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- CN103655809A CN103655809A CN201310679130.8A CN201310679130A CN103655809A CN 103655809 A CN103655809 A CN 103655809A CN 201310679130 A CN201310679130 A CN 201310679130A CN 103655809 A CN103655809 A CN 103655809A
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- artemisiae argyi
- folium artemisiae
- radix sanguisorbae
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- ethyl acetate
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Abstract
The invention relates to a natural composition and a preparation method thereof. The natural composition comprises a folium artemisiae argyi and sanguisorba officinalis decocted concentrated extract, a folium artemisiae argyi ethyl acetate extract and sanguisorba officinalis total saponins according to a ratio of (3-7) to (3-6) to (3-7). The composition is effective for cancer chemotherapy-induced diarrhea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being formed by Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin, the preparation method of this natural drug composition and this natural drug composition purposes in the medicine for the preparation of minimizing cancer chemotherapy induced diarrhea.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Folium Artemisiae Argyi is one of tcm clinical practice common medicine, is the leaf of Compositae sagebruss Artemisiae argyi Led.etVant., and nature and flavor are bitter, pungent, warm, return liver,spleen,kidney warp, has the effects such as pain relieving, antiabortive, dysentery relieving, dissipating depression of QI.Radix Sanguisorbae is the dry root of rosaceous plant Radix Sanguisorbae Sanguisorba officinalis L. or Radix Sanguisorbae Sanguisorba officinalis L. var. longifolia (Bert.) the Y ü et Li that comes into leaves, the dysentery of stopping blooding, < < Japan hanako materia medica > > records Radix Sanguisorbae for blood disorders, dysentery with red and white feces watery diarrhea.The inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and has proved that it has good curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 3-7:3-6:3-7 by weight Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin.
Above-mentioned natural drug composition, wherein the usage ratio of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin is 7:6:7.
Wherein, Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, can be by well known to a person skilled in the art the conventional method acquisition of purifying, the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Folium Artemisiae Argyi, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
In above-mentioned natural drug composition, radix sanguisorbae total saponin preparation method is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Folium Artemisiae Argyi, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
(3) radix sanguisorbae total saponin preparation
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
(4) choose in proportion Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 3-7:3-6:3-7, potentiation is obvious, especially especially obvious when the usage ratio of each component is 7:6:7, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and each component of radix sanguisorbae total saponin all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of natural drug composition of the present invention, with and at the beneficial effect of anti-Chemotherapy-induced Diarrhea.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Folium Artemisiae Argyi, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
(3) radix sanguisorbae total saponin preparation
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract 35mg, Folium Artemisiae Argyi ethyl acetate extract 30mg and radix sanguisorbae total saponin 35mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix filled capsules.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract 35mg, Folium Artemisiae Argyi ethyl acetate extract 30mg and radix sanguisorbae total saponin 35mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix tabletting with 5mg magnesium stearate.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin is 7:6:7.Inventor's compositions that the multiple proportioning combination between 3-7:3-6:3-7 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Folium Artemisiae Argyi, Radix Sanguisorbae
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Folium Artemisiae Argyi ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), D group radix sanguisorbae total saponin 10g.kg
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 3-7:3-6:3-7 by weight Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin,
Wherein the preparation method of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Folium Artemisiae Argyi, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product;
Wherein the preparation method of radix sanguisorbae total saponin is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and radix sanguisorbae total saponin is 7:6:7.
3. the preparation method of natural drug composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Folium Artemisiae Argyi, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product;
(3) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product;
(4) choose in proportion Folium Artemisiae Argyi Radix Sanguisorbae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract evenly mixes with radix sanguisorbae total saponin, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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| CN201310679130.8A CN103655809A (en) | 2013-12-14 | 2013-12-14 | Natural composition and preparation method thereof |
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| CN103655809A true CN103655809A (en) | 2014-03-26 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090010248A (en) * | 2009-01-07 | 2009-01-29 | 지엘팜텍 주식회사 | Artemisia extract |
| CN103340926A (en) * | 2013-07-19 | 2013-10-09 | 石强 | Traditional chinese medicine composition and preparation method thereof |
| CN103393847A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Traditional Chinese medicine composition, preparation method and application thereof |
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- 2013-12-14 CN CN201310679130.8A patent/CN103655809A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090010248A (en) * | 2009-01-07 | 2009-01-29 | 지엘팜텍 주식회사 | Artemisia extract |
| CN103340926A (en) * | 2013-07-19 | 2013-10-09 | 石强 | Traditional chinese medicine composition and preparation method thereof |
| CN103393847A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Traditional Chinese medicine composition, preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 梅全喜: "艾地合剂治疗细菌性痢疾", 《时珍国医国药》 * |
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Application publication date: 20140326 |