CN103690665A - Natural medicine composition - Google Patents
Natural medicine composition Download PDFInfo
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- CN103690665A CN103690665A CN201310673325.1A CN201310673325A CN103690665A CN 103690665 A CN103690665 A CN 103690665A CN 201310673325 A CN201310673325 A CN 201310673325A CN 103690665 A CN103690665 A CN 103690665A
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- fructus schisandrae
- cortex phellodendri
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Abstract
The invention relates to a natural medicine composition which comprises concentration extracts obtained by decocting golden cypress and Chinese magnoliavine fruit by using water, a golden cypress ethyl acetate extract and Chinese magnoliavine fruit polysaccharide at a ratio of (1-6):(5-7):(3-7). The natural medicine composition has a treatment effect on cancer chemotherapy-induced diarrhea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being comprised of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide, the preparation method of this natural drug composition and this natural drug composition are in the purposes for the preparation of reducing in cancer chemotherapy induced diarrhea medicine.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Cortex Phellodendri, is the dry bark of rutaceae wampee or Cortex Phellodendri, energy heat and toxic materials clearing away medicine; Heat clearing and dampness removing medicine.Cure mainly: hygropyretic dysentery, have loose bowels.Fructus Schisandrae Chinensis, is commonly called as Fructus Zanthoxyli Plansipini, sliding weight of steelyard, medicine Fructus Schisandrae Chinensis, face rattan, five Fructus Armeniacae Mumes etc., can restrain astringent or styptic treatment for spontaneous sweating; Supplementing QI for promoting the production of body fluid.Cure mainly: incessant chronic diarrhea.The inventor is through research and the experiment of each a large amount of formula proportion, finally obtained optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can play the effect that suppresses tumor simultaneously cancer chemotherapy induced diarrhea, has completed the present invention thus.
summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 1-6:5-7:3-7 by weight Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide.
Above-mentioned natural drug composition, wherein the usage ratio of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide is 6:7:7.
Wherein, Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract is:
According to proportion compatibility, be that 1-5:1 gets Cortex Phellodendri, Fructus Schisandrae Chinensis, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of Cortex Phellodendri ethyl acetate extract is:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain Cortex Phellodendri ethyl acetate extract.
In above-mentioned natural drug composition, Fructus Schisandrae Polysaccharide preparation method is:
Get Fructus Schisandrae Chinensis dry fruit, pulverize and sieve, with dehydrated alcohol Soxhlet backflow defat, dry, after adding 90 ℃ of lixiviates of water, filter extracting solution concentrating under reduced pressure, 95% alcohol chromatography, centrifugal collecting precipitation, precipitation is used respectively acetone, ether and absolute ethanol washing, and lyophilizing obtains Fructus Schisandrae Polysaccharide.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract
According to proportion compatibility, be that 1-5:1 gets Cortex Phellodendri, Fructus Schisandrae Chinensis, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain Cortex Phellodendri ethyl acetate extract.
(3) Fructus Schisandrae Polysaccharide preparation
Get Fructus Schisandrae Chinensis dry fruit, pulverize and sieve, with dehydrated alcohol Soxhlet backflow defat, dry, after adding 90 ℃ of lixiviates of water, filter extracting solution concentrating under reduced pressure, 95% alcohol chromatography, centrifugal collecting precipitation, precipitation is used respectively acetone, ether and absolute ethanol washing, and lyophilizing obtains Fructus Schisandrae Polysaccharide.
(4) choose in proportion Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 1-6:5-7:3-7, potentiation is obvious, especially especially obvious when the usage ratio of each component is 6:7:7, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and each component of Fructus Schisandrae Polysaccharide all can obtain by conventional means, Fructus Schisandrae Polysaccharide also can be buied by business, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of natural drug composition of the present invention, with and at anti-Chemotherapy-induced Diarrhea beneficial effect.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract
According to proportion compatibility, be that 1-5:1 gets Cortex Phellodendri, Fructus Schisandrae Chinensis, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain Cortex Phellodendri ethyl acetate extract.
(3) Fructus Schisandrae Polysaccharide preparation
Get Fructus Schisandrae Chinensis dry fruit, pulverize and sieve, with dehydrated alcohol Soxhlet backflow defat, dry, after adding 90 ℃ of lixiviates of water, filter extracting solution concentrating under reduced pressure, 95% alcohol chromatography, centrifugal collecting precipitation, precipitation is used respectively acetone, ether and absolute ethanol washing, and lyophilizing obtains Fructus Schisandrae Polysaccharide.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract 30mg, Cortex Phellodendri ethyl acetate extract 35mg and Fructus Schisandrae Polysaccharide 35mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract 30mg, Cortex Phellodendri ethyl acetate extract 35mg and Fructus Schisandrae Polysaccharide 35mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix with 5mg magnesium stearate, be pressed into tablet.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide is 6:7:7.Inventor's compositions that the multiple proportioning combination between 1-6:5-7:3-7 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Cortex Phellodendri, Fructus Schisandrae Chinensis
(2) mice, body weight 1.3-1.8kg, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Cortex Phellodendri ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), D group Fructus Schisandrae Polysaccharide 10g.kg
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 1-6:5-7:3-7 by weight Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide,
Wherein the preparation method of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract is:
According to proportion compatibility, be that 1-5:1 gets Cortex Phellodendri, Fructus Schisandrae Chinensis, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Cortex Phellodendri ethyl acetate extract is:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain;
Wherein the preparation method of Fructus Schisandrae Polysaccharide is:
Get Fructus Schisandrae Chinensis dry fruit, pulverize and sieve, with dehydrated alcohol Soxhlet backflow defat, dry, after adding 90 ℃ of lixiviates of water, filter extracting solution concentrating under reduced pressure, 95% alcohol chromatography, centrifugal collecting precipitation, precipitation is used respectively acetone, ether and absolute ethanol washing, and lyophilizing obtains Fructus Schisandrae Polysaccharide.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Schisandrae Polysaccharide is 6:7:7.
3. the preparation method of natural drug composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract
According to proportion compatibility, be that 1-5:1 gets Cortex Phellodendri, Fructus Schisandrae Chinensis, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain;
(3) preparation of Fructus Schisandrae Polysaccharide
Get Fructus Schisandrae Chinensis dry fruit, pulverize and sieve, with dehydrated alcohol Soxhlet backflow defat, dry, after adding 90 ℃ of lixiviates of water, filter extracting solution concentrating under reduced pressure, 95% alcohol chromatography, centrifugal collecting precipitation, precipitation is used respectively acetone, ether and absolute ethanol washing, and lyophilizing obtains Fructus Schisandrae Polysaccharide;
(4) choose in proportion Cortex Phellodendri Fructus Schisandrae Chinensis water boiling concentration extract, Cortex Phellodendri ethyl acetate extract evenly mixes with Fructus Schisandrae Polysaccharide, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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| CN201310673325.1A CN103690665A (en) | 2013-12-12 | 2013-12-12 | Natural medicine composition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940368A (en) * | 2015-06-05 | 2015-09-30 | 浙江大学 | Drug for preventing and treating honeybee microsporidiosis and use thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1089854A (en) * | 1993-01-15 | 1994-07-27 | 张和祥 | Wet dressing quick-acting antidiarrheal medicine |
| CN101199796A (en) * | 2007-12-14 | 2008-06-18 | 岳联国 | External antidiarrhoeal medicament, preparing method and function thereof |
| CN102048933A (en) * | 2010-12-20 | 2011-05-11 | 崔守凤 | Chinese medicinal composition for treating infantile diarrhea and preparation method thereof |
| CN102294012A (en) * | 2011-09-26 | 2011-12-28 | 荣成市崖头美全口腔诊所 | Chinese medicinal composition for treating diarrhoea |
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- 2013-12-12 CN CN201310673325.1A patent/CN103690665A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1089854A (en) * | 1993-01-15 | 1994-07-27 | 张和祥 | Wet dressing quick-acting antidiarrheal medicine |
| CN101199796A (en) * | 2007-12-14 | 2008-06-18 | 岳联国 | External antidiarrhoeal medicament, preparing method and function thereof |
| CN102048933A (en) * | 2010-12-20 | 2011-05-11 | 崔守凤 | Chinese medicinal composition for treating infantile diarrhea and preparation method thereof |
| CN102294012A (en) * | 2011-09-26 | 2011-12-28 | 荣成市崖头美全口腔诊所 | Chinese medicinal composition for treating diarrhoea |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940368A (en) * | 2015-06-05 | 2015-09-30 | 浙江大学 | Drug for preventing and treating honeybee microsporidiosis and use thereof |
| CN104940368B (en) * | 2015-06-05 | 2018-04-20 | 浙江大学 | A kind of medicine for preventing Nosema apis parasitosis and application thereof |
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Application publication date: 20140402 |