CN103656074A - Traditional Chinese medicine composition - Google Patents
Traditional Chinese medicine composition Download PDFInfo
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- CN103656074A CN103656074A CN201310673298.8A CN201310673298A CN103656074A CN 103656074 A CN103656074 A CN 103656074A CN 201310673298 A CN201310673298 A CN 201310673298A CN 103656074 A CN103656074 A CN 103656074A
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Abstract
The invention relates to a traditional Chinese medicine composition and a preparation method thereof, and application of the traditional Chinese medicine composition in preparation of attenuated synergistic medicines in cancer chemotherapy. The traditional Chinese medicine composition is composed of 2-4 parts of dark plum/Chinese yam water decoction concentrated extract, 2-5 parts of dark plum ethanol extract, 5-9 parts of Chinese yam ethanol extract and 1-4 parts of Chinese yam polysaccharide. The composition has curative effect on cancer chemotherapy related diarrhoea.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition being comprised of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, the preparation method of this Chinese medicine composition and this Chinese medicine composition reduce the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Fructus Mume is that the dry almost ripe fruit by rose department plant plum processes, can astringe the lung, and astringing intestine to stop diarrhea.For chronic diarrhea; Dysentery; The diseases such as vomiting.Rhizoma Dioscoreae is perennial voluble herb, Chinese medicine, vegetable dual-purpose, and motherland's medical science thinks, the multiple efficacies such as Rhizoma Dioscoreae has spleen invigorating, tonifying the lung, reinforces the kidney, benefit essence, have therapeutical effect to diarrhea due to hypofunction of the spleen.The inventor is through research and the experiment of each a large amount of formula proportion, finally obtained optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can play the effect that suppresses tumor simultaneously cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of Chinese medicine composition provided by the invention, consists of in the ratio of 2-4:2-5:5-9:1-4 by weight Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 4:9:3:9.
Wherein, Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned Chinese medicine composition, the preparation method of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Fructus Mume, Rhizoma Dioscoreae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned Chinese medicine composition, the preparation method of Fructus Mume ethanol extraction is:
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux of 8 times of amounts 12 hours, filter, medicinal residues add 75% alcohol reflux 6 hours of 6 times of amounts, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverize, sieve, obtain.
In above-mentioned Chinese medicine composition, Rhizoma Dioscoreae ethanol extraction preparation method is:
Get dry Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h, collect extracting solution, centrifugal, collect supernatant, by supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, obtains.
In above-mentioned Chinese medicine composition, Chinese yam polysaccharide preparation method is:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added to water redissolution and be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization, obtains white Chinese yam polysaccharide.
The present invention also provides the preferred preparation method of this Chinese medicine composition, comprising:
(1) preparation of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Fructus Mume, Rhizoma Dioscoreae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Fructus Mume ethanol extraction
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux of 8 times of amounts 12 hours, filter, medicinal residues add 75% alcohol reflux 6 hours of 6 times of amounts, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverize, sieve, obtain.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get dry Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h, collect extracting solution, centrifugal, collect supernatant, by supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, obtains.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added to water redissolution and be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization, obtains white Chinese yam polysaccharide.
(5) choose in proportion Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, evenly mix, obtain.
The preparation method of above-mentioned Chinese medicine composition, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
Chinese medicine composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, Chinese medicine composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 2-4:2-5:5-9:1-4, potentiation is obvious, especially especially obvious when the usage ratio of each component is 4:9:3:9, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this Chinese medicine composition Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and each component of Chinese yam polysaccharide all can obtain by conventional means, Chinese yam polysaccharide also can be buied by business, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of Chinese medicine composition of the present invention, with and at anti-Chemotherapy-induced Diarrhea beneficial effect.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Fructus Mume, Rhizoma Dioscoreae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Fructus Mume ethanol extraction
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux of 8 times of amounts 12 hours, filter, medicinal residues add 75% alcohol reflux 6 hours of 6 times of amounts, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverize, sieve, obtain.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get dry Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h, collect extracting solution, centrifugal, collect supernatant, by supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, obtains.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added to water redissolution and be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization, obtains white Chinese yam polysaccharide.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take respectively Fructus Mume Rhizoma Dioscoreae water boiling concentration extract 16mg, Fructus Mume ethanol extraction 36mg, Rhizoma Dioscoreae ethanol extraction 12mg and Chinese yam polysaccharide 36mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix filled capsules.
(2) preparation of Chinese medicine composition tablet of the present invention
Take respectively Fructus Mume Rhizoma Dioscoreae water boiling concentration extract 16mg, Fructus Mume ethanol extraction 36mg, Rhizoma Dioscoreae ethanol extraction 12mg and Chinese yam polysaccharide 36mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix tabletting with 5mg magnesium stearate.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 4:9:3:9.Inventor's compositions that the multiple proportioning combination between 3-4:6-9:2-3:4-9 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the Chinese medicine composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of traditional Chinese medicine composition for treating Chemotherapy-induced Diarrhea effect
1, test material
(1) Fructus Mume, Rhizoma Dioscoreae, is purchased from medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Fructus Mume Rhizoma Dioscoreae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Fructus Mume ethanol extraction 10g.kg
-1(being equivalent to containing crude drug amount), D group Rhizoma Dioscoreae ethanol extraction mixes 10g.kg with Chinese yam polysaccharide in 1:3 ratio
-1(being equivalent to containing crude drug amount), E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, Chinese medicine composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount
Claims (5)
1. a Chinese medicine composition that reduces cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-4:2-5:5-9:1-4 by weight Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide
Wherein the preparation method of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Fructus Mume, Rhizoma Dioscoreae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Fructus Mume ethanol extraction is:
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux, filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and obtains;
Wherein the preparation method of Rhizoma Dioscoreae ethanol extraction is:
Get dry Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h, collect extracting solution, centrifugal, collect supernatant, by supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, obtains;
Wherein the preparation method of Chinese yam polysaccharide is:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added to water redissolution and be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation, collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, uses respectively ethanol, acetone and ether washing precipitation, lyophilization, obtains white Chinese yam polysaccharide.
2. Chinese medicine composition as claimed in claim 1, wherein the usage ratio of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 4:9:3:9.
3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Fructus Mume Rhizoma Dioscoreae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Fructus Mume, Rhizoma Dioscoreae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Fructus Mume ethanol extraction
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux, filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and obtains;
(3) preparation of Rhizoma Dioscoreae ethanol extraction
Get dry Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h, collect extracting solution, centrifugal, collect supernatant, by supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, obtains;
(4) preparation of Chinese yam polysaccharide
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added to water redissolution and be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization, obtains white Chinese yam polysaccharide;
(5) choose in proportion Fructus Mume Rhizoma Dioscoreae water boiling concentration extract, Fructus Mume ethanol extraction, Rhizoma Dioscoreae ethanol extraction and evenly mix with Chinese yam polysaccharide, obtain.
4. the preparation method of Chinese medicine composition as claimed in claim 3, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
5. the Chinese medicine composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108014138A (en) * | 2016-10-31 | 2018-05-11 | 佳木斯大学 | A kind of medicine that can adjust intestinal bacilli illness enhancing immunization |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106952A (en) * | 2010-04-16 | 2011-06-29 | 胡海舰 | Infused drink for treating chronic diarrhea |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106952A (en) * | 2010-04-16 | 2011-06-29 | 胡海舰 | Infused drink for treating chronic diarrhea |
Non-Patent Citations (1)
| Title |
|---|
| 王文成等: "中医药治疗化疗相关性腹泻的研究述略", 《实用中医内科杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108014138A (en) * | 2016-10-31 | 2018-05-11 | 佳木斯大学 | A kind of medicine that can adjust intestinal bacilli illness enhancing immunization |
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Application publication date: 20140326 |