CN103656432A - Traditional chinese medicine composition and preparation method thereof - Google Patents
Traditional chinese medicine composition and preparation method thereof Download PDFInfo
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- CN103656432A CN103656432A CN201310673297.3A CN201310673297A CN103656432A CN 103656432 A CN103656432 A CN 103656432A CN 201310673297 A CN201310673297 A CN 201310673297A CN 103656432 A CN103656432 A CN 103656432A
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- alpiniae katsumadai
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Abstract
The invention relates to a traditional Chinese medicine composition and a preparation method thereof. The traditional Chinese medicine composition is composed of 2-6 parts of Amomum globosum/dark plum water decoction concentrated extract, 5-9 parts of Amomum globosum ethyl acetate extract and 3-5 parts of dark plum ethanol extract. The composition has curative effect on cancer chemotherapy related diarrhoea.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition being comprised of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction, the preparation method of this Chinese medicine composition and this Chinese medicine composition reduce the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea natural Chinese medicine becomes clinical problem demanding prompt solution.
Semen Alpiniae Katsumadai is the seed of zingiberaceous plant Semen Alpiniae Katsumadai Alpinia katsumadai Hayata.The < < Compendium of Materia Medica > > of Ming Dynasty's Li Shizhen (1518-1593 A.D.) supplements its effect for can " summer-heat that cures the wound is told lower leakage dysentery; dysphagia and regurgitation; feeling of fullness acid regurgitation " etc., the coldness and pain in the epigastrium of the cloudy stagnant taste of main cold-damp; Feeling of fullness is done swollen, vomiting; The disease such as have loose bowels.Fructus Mume is that the dry almost ripe fruit by rose department plant plum processes, can astringe the lung, and astringing intestine to stop diarrhea.For chronic diarrhea; Dysentery; The diseases such as vomiting.The inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and has proved that it has good curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of Chinese medicine composition provided by the invention, consists of in the ratio of 2-6:5-9:3-5 by weight Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction is 6:9:5.
Wherein, Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned Chinese medicine composition, the preparation method of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Fructus Mume, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned Chinese medicine composition, the preparation method of Semen Alpiniae Katsumadai ethyl acetate extract is:
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain.
In above-mentioned Chinese medicine composition, Fructus Mume ethanol extraction preparation method is:
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux of 8 times of amounts 12 hours, filter, medicinal residues add 75% alcohol reflux 6 hours of 6 times of amounts, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverize, sieve, obtain.
The present invention also provides the preferred preparation method of this Chinese medicine composition, comprising:
(1) preparation of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Fructus Mume, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain.
(3) Fructus Mume ethanol extraction preparation
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux of 8 times of amounts 12 hours, filter, medicinal residues add 75% alcohol reflux 6 hours of 6 times of amounts, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverize, sieve, obtain.
(4) choose in proportion Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction, evenly mix, obtain.
The preparation method of above-mentioned Chinese medicine composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
Chinese medicine composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, Chinese medicine composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 2-6:5-9:3-5, potentiation is obvious, especially especially obvious when the usage ratio of each component is 6:9:5, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this Chinese medicine composition Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and each component of Fructus Mume ethanol extraction all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of Chinese medicine composition of the present invention, with and at anti-Chemotherapy-induced Diarrhea beneficial effect.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Fructus Mume, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain.
(3) Fructus Mume ethanol extraction preparation
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux of 8 times of amounts 12 hours, filter, medicinal residues add 75% alcohol reflux 6 hours of 6 times of amounts, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverize, sieve, obtain.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take respectively Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract 30mg, Semen Alpiniae Katsumadai ethyl acetate extract 45mg and Fructus Mume ethanol extraction 25mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix filled capsules.
(2) preparation of Chinese medicine composition tablet of the present invention
Take respectively Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract 30mg, Semen Alpiniae Katsumadai ethyl acetate extract 45mg and Fructus Mume ethanol extraction 25mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix tabletting with 5mg magnesium stearate.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction is 6:9:5.Inventor's compositions that the multiple proportioning combination between 2-6:5-9:3-5 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the Chinese medicine composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of traditional Chinese medicine composition for treating Chemotherapy-induced Diarrhea effect
1, test material
(1) Semen Alpiniae Katsumadai, Fructus Mume
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Semen Alpiniae Katsumadai ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), D group Fructus Mume ethanol extraction 10g.kg
-1(being equivalent to containing crude drug amount), E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, Chinese medicine composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a Chinese medicine composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-6:5-9:3-5 by weight Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction,
Wherein the preparation method of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Fructus Mume, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Semen Alpiniae Katsumadai ethyl acetate extract is:
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain;
Wherein the preparation method of Fructus Mume ethanol extraction is:
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux, filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and obtains.
2. Chinese medicine composition as claimed in claim 1, wherein the usage ratio of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and Fructus Mume ethanol extraction is 6:9:5.
3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Fructus Mume, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain;
(3) preparation of Fructus Mume ethanol extraction
Dry Fructus Mume is pulverized, sieved, 75% alcohol reflux, filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and obtains;
(4) choose in proportion Semen Alpiniae Katsumadai Fructus Mume water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract evenly mixes with Fructus Mume ethanol extraction, obtain.
4. the preparation method of Chinese medicine composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
5. the Chinese medicine composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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| CN103223133A (en) * | 2013-03-27 | 2013-07-31 | 张宗升 | Traditional chinese medicine composition |
| CN103223049A (en) * | 2013-04-08 | 2013-07-31 | 张宗升 | Chinese medicinal extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103223133A (en) * | 2013-03-27 | 2013-07-31 | 张宗升 | Traditional chinese medicine composition |
| CN103223049A (en) * | 2013-04-08 | 2013-07-31 | 张宗升 | Chinese medicinal extract |
Non-Patent Citations (3)
| Title |
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| 周兰: "中药辨治配合敷脐剂治疗恶性肿瘤放化疗后腹泻疗效观察", 《辽宁中医杂志》 * |
| 王文成等: "中医药治疗化疗相关性腹泻的研究述略", 《实用中医内科杂志》 * |
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Application publication date: 20140326 |