CN103655975A - Traditional Chinese medicine composition - Google Patents
Traditional Chinese medicine composition Download PDFInfo
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- CN103655975A CN103655975A CN201310668130.8A CN201310668130A CN103655975A CN 103655975 A CN103655975 A CN 103655975A CN 201310668130 A CN201310668130 A CN 201310668130A CN 103655975 A CN103655975 A CN 103655975A
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- fructus chebulae
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Abstract
The invention relates to a traditional Chinese medicine composition. The traditional Chinese medicine composition is composed of 2-9 parts of rhizoma alismatis/myrobalam water decoction concentrated extract, 3-5 parts of rhizoma alismatis terpineol B acetate and 5-6 parts of myrobalam 70% ethanol extract. The composition has curative effect on cancer chemotherapy related diarrhoea.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition being comprised of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction, the preparation method of this Chinese medicine composition and this Chinese medicine composition are for the preparation of reducing cancer chemotherapy induced diarrhea and treatment of cancer being played to the purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
The Rhizoma Alismatis beginning is loaded in < < Sheng Nong's herbal classic > >, classify as top grade, " sweet in the mouth, temperature, tonify deficiency wins, except cold and heat pathogen, invigorating the spleen and replenishing QI power, longue meat, take normal hearing and eyesight for a long time to call it.Cure mainly diarrhea due to hypofunction of the spleen.Fructus Chebulae, calls tender Fructus Chebulae, chebule, scolds multitude, scolds pears, son with the wind.Sour in the mouth is puckery; Property is put down, and returns lung, large intestine channel; Having relieving diarrhea with astringents effect, is to control chronic diarrhea; Chronic dysentery; The common medicine of proctoptosis, the inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and proved that it has fabulous curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of Chinese medicine composition provided by the invention, consists of in the ratio of 2-9:3-5:5-6 by weight Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction is 9:5:6.
Wherein, Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas, can be by well known to a person skilled in the art the conventional method acquisition of purifying, the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned Chinese medicine composition, the preparation method of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Rhizoma Alismatis, Fructus Chebulae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned Chinese medicine composition, the preparation method of alisol B acetas is:
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain alisol B acetas.
In above-mentioned Chinese medicine composition, Fructus Chebulae's 70% ethanol extraction preparation method is:
Get Fructus Chebulae, dry, pulverize, sieve, with 7-10 times of 70% soak with ethanol 12h, cross leaching clear liquor, residue continues to soak 12h, twice filtrate is merged to centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, then drying under reduced pressure becomes dry extract, pulverizes, and crosses 100 mesh sieves, obtains.
The present invention also provides the preferred preparation method of this Chinese medicine composition, comprising:
(1) preparation of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Rhizoma Alismatis, Fructus Chebulae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of alisol B acetas
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain alisol B acetas.
(3) Fructus Chebulae's 70% ethanol extraction preparation
Get Fructus Chebulae, dry, pulverize, sieve, with 7-10 times of 70% soak with ethanol 12h, cross leaching clear liquor, residue continues to soak 12h, twice filtrate is merged to centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, then drying under reduced pressure becomes dry extract, pulverizes, and crosses 100 mesh sieves, obtains.
(4) choose in proportion Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction, evenly mix, obtain.
The preparation method of above-mentioned Chinese medicine composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
Chinese medicine composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, Chinese medicine composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 2-9:3-5:5-6, potentiation is obvious, especially especially obvious when the usage ratio of each component is 9:5:6, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this Chinese medicine composition Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and each component of Fructus Chebulae's 70% ethanol extraction all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of Chinese medicine composition of the present invention, with and at anti-Chemotherapy-induced Diarrhea beneficial effect.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Rhizoma Alismatis, Fructus Chebulae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of alisol B acetas
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain alisol B acetas.
(3) Fructus Chebulae's 70% ethanol extraction preparation
Get Fructus Chebulae, dry, pulverize, sieve, with 7-10 times of 70% soak with ethanol 12h, cross leaching clear liquor, residue continues to soak 12h, twice filtrate is merged to centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, then drying under reduced pressure becomes dry extract, pulverizes, and crosses 100 mesh sieves, obtains.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take respectively Rhizoma Alismatis Fructus Chebulae water boiling concentration extract 45mg, alisol B acetas 25mg and Fructus Chebulae 70% ethanol extraction 30mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of Chinese medicine composition tablet of the present invention
Take respectively Rhizoma Alismatis Fructus Chebulae water boiling concentration extract 45mg, alisol B acetas 25mg and Fructus Chebulae 70% ethanol extraction 30mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix with 5mg magnesium stearate, be pressed into tablet.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction is 9:5:6.Inventor's compositions that the multiple proportioning combination between 2-9:3-5:5-6 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the Chinese medicine composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of traditional Chinese medicine composition for treating Chemotherapy-induced Diarrhea effect
1, test material
(1) Rhizoma Alismatis, Fructus Chebulae
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Rhizoma Alismatis Fructus Chebulae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is alisol B acetas 10g.kg
-1(being equivalent to containing crude drug amount), D group Fructus Chebulae 70% ethanol extraction 10g.kg
-1(being equivalent to containing crude drug amount), E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, Chinese medicine composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a Chinese medicine composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-9:3-5:5-6 by weight Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction,
Wherein the preparation method of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Rhizoma Alismatis, Fructus Chebulae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of alisol B acetas is:
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain;
Wherein the preparation method of Fructus Chebulae's 70% ethanol extraction is:
Get Fructus Chebulae, dry, pulverize, sieve, 70% soak with ethanol, crosses leaching clear liquor, and residue continues to soak 12h, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
2. Chinese medicine composition as claimed in claim 1, wherein the usage ratio of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas and Fructus Chebulae's 70% ethanol extraction is 9:5:6.
3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Rhizoma Alismatis Fructus Chebulae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Rhizoma Alismatis, Fructus Chebulae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of alisol B acetas
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain;
(3) preparation of Fructus Chebulae's 70% ethanol extraction
Get Fructus Chebulae, dry, pulverize, sieve, 70% soak with ethanol, crosses leaching clear liquor, and residue continues to soak 12h, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains;
(4) choose in proportion Rhizoma Alismatis Fructus Chebulae water boiling concentration extract, alisol B acetas evenly mixes with Fructus Chebulae's 70% ethanol extraction, obtain.
4. the preparation method of Chinese medicine composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
5. the Chinese medicine composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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Application publication date: 20140326 |