CN103690706A - Natural pharmaceutical composition as well as preparation method and use thereof - Google Patents
Natural pharmaceutical composition as well as preparation method and use thereof Download PDFInfo
- Publication number
- CN103690706A CN103690706A CN201310663286.7A CN201310663286A CN103690706A CN 103690706 A CN103690706 A CN 103690706A CN 201310663286 A CN201310663286 A CN 201310663286A CN 103690706 A CN103690706 A CN 103690706A
- Authority
- CN
- China
- Prior art keywords
- ethyl acetate
- artemisiae argyi
- folium artemisiae
- extract
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a natural pharmaceutical composition as well as a preparation method and a use thereof. The natural pharmaceutical composition is composed of an alisma mugwort-moxa leaf decoction concentrate extract, orientalol B acetate and a moxa leaf ethyl acetate extract in a ratio of (5-9): (1-3): (4-8); the composition has good curative effect on cancer chemotherapy-associated diarrhea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being formed by Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract, the preparation method of this natural drug composition and this natural drug composition purposes in the medicine for the preparation of minimizing cancer chemotherapy induced diarrhea.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly that the rehabilitation of body is brought, and to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, can be used for of being easy to promote alleviate cancer chemotherapy induced diarrhea and natural Chinese medicine become clinical problem demanding prompt solution.
The Rhizoma Alismatis beginning is loaded in < < Sheng Nong's herbal classic > >, classify as top grade, " sweet in the mouth, temperature, tonify deficiency wins, except cold and heat pathogen, invigorating the spleen and replenishing QI power, longue meat, take normal hearing and eyesight for a long time to call it.Cure mainly diarrhea due to hypofunction of the spleen.Folium Artemisiae Argyi is one of tcm clinical practice common medicine, is the leaf of Compositae sagebruss Artemisiae argyi Led.etVant., in China most areas, has distribution.Folium Artemisiae Argyi nature and flavor are bitter, pungent, warm, return liver,spleen,kidney warp, have the effects such as pain relieving, antiabortive, dysentery relieving, dissipating depression of QI.The inventor is through research and the experiment of each a large amount of formula proportion, finally obtained optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can play the effect that suppresses tumor simultaneously cancer chemotherapy induced diarrhea, has completed the present invention thus.
summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 5-9:1-3:4-8 by weight Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract.
Above-mentioned natural drug composition, wherein the usage ratio of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract is 9:3:8.
Wherein, Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas, can be by well known to a person skilled in the art the conventional method acquisition of purifying, the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract is:
According to proportion compatibility, be that 1-5:1 gets Rhizoma Alismatis, Folium Artemisiae Argyi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of alisol B acetas is:
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain.
In above-mentioned natural drug composition, Folium Artemisiae Argyi ethyl acetate extract preparation method is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract
According to proportion compatibility, be that 1-5:1 gets Rhizoma Alismatis, Folium Artemisiae Argyi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of alisol B acetas
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain alisol B acetas.
(3) Folium Artemisiae Argyi ethyl acetate extract preparation
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
(4) choose in proportion Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor is originally by Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas, combine with Folium Artemisiae Argyi ethyl acetate extract, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 5-9:1-3:4-8, potentiation is obvious, especially especially obvious when the usage ratio of each component is 9:3:8, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and each component of Folium Artemisiae Argyi ethyl acetate extract all can obtain by conventional means, component also can be buied by business as Folium Artemisiae Argyi ethyl acetate extract, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Embodiment
The first step: the preparation of various extracts
(1) preparation of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract
According to proportion compatibility, be that 1-5:1 gets Rhizoma Alismatis, Folium Artemisiae Argyi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of alisol B acetas
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain alisol B acetas.
(3) Folium Artemisiae Argyi ethyl acetate extract preparation
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract 45mg, alisol B acetas 15mg, Folium Artemisiae Argyi ethyl acetate extract 40mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract 45mg, alisol B acetas 15mg, Folium Artemisiae Argyi ethyl acetate extract 40mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix with 5mg magnesium stearate, be pressed into tablet.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract is 9:3:8.Inventor's compositions that the multiple proportioning combination between 5-9:1-3:4-8 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Rhizoma Alismatis, Folium Artemisiae Argyi, is purchased from medical material market.
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is alisol B acetas 10g.kg
-1(being equivalent to containing crude drug amount), D group Folium Artemisiae Argyi ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 5-9:1-3:4-8 by weight Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract,
Wherein the preparation method of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract is:
According to proportion compatibility, be that 1-5:1 gets Rhizoma Alismatis, Folium Artemisiae Argyi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of alisol B acetas is:
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain;
Wherein the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas and Folium Artemisiae Argyi ethyl acetate extract is 9:3:8.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract
According to proportion compatibility, be that 1-5:1 gets Rhizoma Alismatis, Folium Artemisiae Argyi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of alisol B acetas
By Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain;
(3) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product;
(4) choose in proportion Rhizoma Alismatis Folium Artemisiae Argyi water boiling concentration extract, alisol B acetas evenly mixes with Folium Artemisiae Argyi ethyl acetate extract, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310663286.7A CN103690706A (en) | 2013-12-10 | 2013-12-10 | Natural pharmaceutical composition as well as preparation method and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310663286.7A CN103690706A (en) | 2013-12-10 | 2013-12-10 | Natural pharmaceutical composition as well as preparation method and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103690706A true CN103690706A (en) | 2014-04-02 |
Family
ID=50352476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310663286.7A Pending CN103690706A (en) | 2013-12-10 | 2013-12-10 | Natural pharmaceutical composition as well as preparation method and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103690706A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101745092A (en) * | 2010-02-09 | 2010-06-23 | 黄美蓉 | Traditional Chinese medicine for treating infantile diarrhea |
-
2013
- 2013-12-10 CN CN201310663286.7A patent/CN103690706A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101745092A (en) * | 2010-02-09 | 2010-06-23 | 黄美蓉 | Traditional Chinese medicine for treating infantile diarrhea |
Non-Patent Citations (1)
| Title |
|---|
| 姚成云: "化疗相关性腹泻的防治", 《肿瘤基础与临床》, vol. 22, no. 4, 31 August 2009 (2009-08-31), pages 364 - 366 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103181959A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103385830A (en) | Traditional Chinese medicine composition and its preparation method and use | |
| CN103690634A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103223066A (en) | Chinese medicinal extract | |
| CN103239529A (en) | Traditional chinese medicine composition | |
| CN103340970A (en) | Traditional chinese medicine composition | |
| CN103223082A (en) | Extract product of traditional Chinese medicine | |
| CN103655731A (en) | Natural pharmaceutical composition | |
| CN103690706A (en) | Natural pharmaceutical composition as well as preparation method and use thereof | |
| CN103656433A (en) | Natural pharmaceutical composition as well as preparation method and application thereof | |
| CN103656074A (en) | Traditional Chinese medicine composition | |
| CN103655806A (en) | Natural medicine composition as well as preparation method and application thereof | |
| CN103301225A (en) | Natural pharmaceutical composition | |
| CN103263456B (en) | Traditional chinese medicine composition | |
| CN103394014A (en) | Natural medicine composition | |
| CN103655859A (en) | Natural pharmaceutical composition | |
| CN103223135A (en) | Traditional chinese medicine composition | |
| CN103223136A (en) | Traditional Chinese medicine extract and its preparation method | |
| CN103655974A (en) | Natural pharmaceutical composition, and preparation method and applications thereof | |
| CN103655976A (en) | Natural composition and preparation method thereof | |
| CN103655977A (en) | Natural pharmaceutical composition | |
| CN103690584A (en) | Natural medicine composition as well as preparation method and application thereof | |
| CN103655809A (en) | Natural composition and preparation method thereof | |
| CN103655837A (en) | Traditional chinese medicine composition | |
| CN103656426A (en) | Natural pharmaceutical composition and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140402 |