CN103223136A - Traditional Chinese medicine extract and its preparation method - Google Patents
Traditional Chinese medicine extract and its preparation method Download PDFInfo
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- CN103223136A CN103223136A CN201310132493XA CN201310132493A CN103223136A CN 103223136 A CN103223136 A CN 103223136A CN 201310132493X A CN201310132493X A CN 201310132493XA CN 201310132493 A CN201310132493 A CN 201310132493A CN 103223136 A CN103223136 A CN 103223136A
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Abstract
The invention relates to a traditional Chinese medicine extract and its preparation method. The natural medicinal extract consists of an Alpinia katsumadai-semen raphani water decocted concentrated extract, Alpinia katsumadai ethyl acetate extract and sulforaphane in a ratio of 2-3:5-9:5-8. The extract has a very good curative effect on cancer chemotherapy related diarrhea and also can achieve a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin, the preparation method of this natural drug composition and this natural drug composition are being used for preparing the purposes that reduces cancer chemotherapy dependency diarrhoea and treatment of cancer is played the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.The chemotherapy prescription with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan etc. in recent years, chemotherapy dependency diarrheal incidence rate improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, therefore also bring certain difficulty, to the important component part of chemotherapy dependency diarrheal effective treatment having become tumor patient wholistic therapy to CC.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but still unmatchful anticancer disease chemotherapy has the Chinese patent medicine of alleviating diarrhoea and potentiation at present.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Semen Alpiniae Katsumadai is the seed of zingiberaceous plant Semen Alpiniae Katsumadai, and Compendium of Material Medica is put down in writing its effect for can " summer-heat that cures the wound is told down leakage dysentery, dysphagia and regurgitation, feeling of fullness acid regurgitation " etc., disease such as is used for vomitting, have loose bowels.Semen Raphani is the dry mature seed of crucifer Radix Raphani, is used for the treatment of retention of food and drink, abdominal distention, stagnant dysentery.In recent years, Semen Alpiniae Katsumadai and Semen Raphani antitumaous effect are confirmed, the inventor is through the research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, and proved that it has fabulous curative effect and can finish the present invention thus simultaneously to chemotherapeutic sensitivity cancer chemotherapy dependency diarrhoea.
Summary of the invention
A kind of natural drug composition provided by the invention, by Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin by weight in 2-3: the ratio of 5-9: 5-8 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin is 3: 9: 8.
Wherein, Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract, can be by well known to a person skilled in the art the method acquisition of purifying, the natural drug of the present invention that these compositions that conventional method obtains or the commercial one-tenth of buying are grouped into all can obtain identical curative effect.
Preferably, Semen Alpiniae Katsumadai Semen Raphani water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
According to proportion compatibility is 1-4: 1 gets Semen Alpiniae Katsumadai, Semen Raphani, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, promptly.
The preparation method of Semen Alpiniae Katsumadai ethyl acetate extract is in the above-mentioned natural drug composition:
Semen Alpiniae Katsumadai is pulverized, and sieves, and with 70% industrial alcohol heating and refluxing extraction 3 times, each 2h merges solvent, reclaim under reduced pressure, dry extract, extractum is put to room temperature after being diluted to suspension with hot water, ethyl acetate extraction reclaims solvent, drying, pulverizing is sieved, promptly.
The raphanin preparation method is in the above-mentioned natural drug composition:
Semen Raphani grinds and adds excessive defat with n-hexane, phosphate buffer enzymolysis with pH7.0, add dichloromethane extraction respectively 3 times, merge solvent, organic solvent is removed in decompression, residue volume fraction 10% dissolve with ethanol solution, n-hexane extraction 3 times is to remove degrease, again ethanol is used dichloromethane extraction 3 times mutually, merge solvent, rotary evaporation is removed dichloromethane promptly.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract
According to proportion compatibility is 1-4: 1 gets Semen Alpiniae Katsumadai, Semen Raphani, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, promptly.
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Semen Alpiniae Katsumadai is pulverized, and sieves, and with 70% industrial alcohol heating and refluxing extraction 3 times, each 2h merges solvent, reclaim under reduced pressure, dry extract, extractum is put to room temperature after being diluted to suspension with hot water, ethyl acetate extraction reclaims solvent, drying, pulverizing is sieved, promptly.
(3) raphanin preparation
Semen Raphani grinds and adds excessive defat with n-hexane, phosphate buffer enzymolysis with pH7.0, add dichloromethane extraction respectively 3 times, merge solvent, organic solvent is removed in decompression, residue volume fraction 10% dissolve with ethanol solution, n-hexane extraction 3 times is to remove degrease, again ethanol is used dichloromethane extraction 3 times mutually, merge solvent, rotary evaporation is removed dichloromethane promptly.
(4) get Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin in proportion, uniform mixing, promptly.
The preparation method of above-mentioned natural drug composition also comprises: the product that step (4) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen the inhibitory action of chemotherapeutics to tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined inhibitory action mouse interior tumor.Studies show that: this natural drug composition can obviously increase the inhibitory action of cyclophosphamide for tumor.
3, the inventor is originally with Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin make up, and by the consumption proportion between definite these components of a large amount of experiments, make said composition performance synergism, through experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-3: 5-9: produce synergism during 5-8, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio of each component be 3: 9: 8 o'clock especially obvious, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and each component of raphanin all can obtain by conventional means, wherein component such as raphanin also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract
According to proportion compatibility is 1-4: 1 gets Semen Alpiniae Katsumadai, Semen Raphani, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, promptly.
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Semen Alpiniae Katsumadai is pulverized, and sieves, and with 70% industrial alcohol heating and refluxing extraction 3 times, each 2h merges solvent, reclaim under reduced pressure, dry extract, extractum is put to room temperature after being diluted to suspension with hot water, ethyl acetate extraction reclaims solvent, drying, pulverizing is sieved, promptly.
(3) raphanin preparation
Semen Raphani grinds and adds excessive defat with n-hexane, phosphate buffer enzymolysis with pH7.0, add dichloromethane extraction respectively 3 times, merge solvent, organic solvent is removed in decompression, residue volume fraction 10% dissolve with ethanol solution, n-hexane extraction 3 times is to remove degrease, again ethanol is used dichloromethane extraction 3 times mutually, merge solvent, rotary evaporation is removed dichloromethane promptly.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract 15mg, Semen Alpiniae Katsumadai ethyl acetate extract 45mg and the raphanin 40mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, uniform mixing is filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract 15mg, Semen Alpiniae Katsumadai ethyl acetate extract 40mg and the raphanin 40mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, uniform mixing, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin is 3: 9: 8.The inventor to above-mentioned each composition usage ratio at 2-3: 5-9: the compositions that the multiple proportioning combination in the time of between the 5-8 obtains has been carried out and last identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Semen Alpiniae Katsumadai, Semen Raphani
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Semen Alpiniae Katsumadai ethyl acetate extract 10g.kg
-1(being equivalent to contain the crude drug amount), D group raphanin 10g.kg
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, duplicates chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract group chemotherapy dependency diarrheal degree is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin by weight in 2-3: the ratio of 5-9: 5-8 is formed
Wherein Semen Alpiniae Katsumadai Semen Raphani water boiling concentration preparation method of extract is:
According to proportion compatibility is 1-4: 1 gets Semen Alpiniae Katsumadai, Semen Raphani, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, promptly;
Wherein the preparation method of Semen Alpiniae Katsumadai ethyl acetate extract is:
Semen Alpiniae Katsumadai is pulverized, and sieves, and with 70% industrial alcohol heating and refluxing extraction 3 times, each 2h merges solvent, reclaim under reduced pressure, dry extract, extractum is put to room temperature after being diluted to suspension with hot water, ethyl acetate extraction reclaims solvent, drying, pulverizing is sieved, promptly;
Wherein the preparation method of raphanin is:
Semen Raphani grinds and adds excessive defat with n-hexane, phosphate buffer enzymolysis with pH7.0, add dichloromethane extraction respectively 3 times, merge solvent, organic solvent is removed in decompression, residue volume fraction 10% dissolve with ethanol solution, n-hexane extraction 3 times is to remove degrease, again ethanol is used dichloromethane extraction 3 times mutually, merge solvent, rotary evaporation is removed dichloromethane promptly.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin is 3: 9: 8.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract
According to proportion compatibility is 1-4: 1 gets Semen Alpiniae Katsumadai, Semen Raphani, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, promptly;
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Semen Alpiniae Katsumadai is pulverized, and sieves, and with 70% industrial alcohol heating and refluxing extraction 3 times, each 2h merges solvent, reclaim under reduced pressure, dry extract, extractum is put to room temperature after being diluted to suspension with hot water, ethyl acetate extraction reclaims solvent, drying, pulverizing is sieved, promptly;
(3) preparation of raphanin
Semen Raphani grinds and adds excessive defat with n-hexane, phosphate buffer enzymolysis with pH7.0, add dichloromethane extraction respectively 3 times, merge solvent, organic solvent is removed in decompression, residue volume fraction 10% dissolve with ethanol solution, n-hexane extraction 3 times is to remove degrease, again ethanol is used dichloromethane extraction 3 times mutually, merge solvent, rotary evaporation is removed dichloromethane promptly;
(4) choose Semen Alpiniae Katsumadai Semen Raphani water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and raphanin uniform mixing in proportion, promptly.
4. the preparation method of natural drug composition as claimed in claim 3 is characterized in that also comprising: the product that step (4) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655807A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition |
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2013
- 2013-04-08 CN CN201310132493XA patent/CN103223136A/en active Pending
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| 唐俊 等: "草豆蔻种子化学成分及其NF-κB的激活抑制作用与抗肿瘤活性", 《中国中药杂志》 * |
| 赵婧 等: "莱菔子的现代研究及临床应用", 《长春中医药大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655807A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition |
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Application publication date: 20130731 |