CN103223036A - Traditional Chinese medicinal composition - Google Patents
Traditional Chinese medicinal composition Download PDFInfo
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- CN103223036A CN103223036A CN2013101719898A CN201310171989A CN103223036A CN 103223036 A CN103223036 A CN 103223036A CN 2013101719898 A CN2013101719898 A CN 2013101719898A CN 201310171989 A CN201310171989 A CN 201310171989A CN 103223036 A CN103223036 A CN 103223036A
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- pericarpium granati
- radix sanguisorbae
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Abstract
The invention relates to a natural medicinal composition, its preparation method and its use in the preparation of medicines for the toxicity reduction and efficacy enhancement in the cancer chemotherapy. The natural medicinal composition is composed of a concentrate of an extract obtained through decocting Radix Sanguisorbae and Pomegranate Rind in water, Radix Sanguisorbae total saponins, an ethanol extract of Pomegranate Rind, and Pomegranate Rind tannins according to a ratio of 2-8:1-2:3-7:5-8. The composition has a very good curative effect on cancer chemotherapy related diarrhea, and simultaneously has a synergistic effect on the cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin, the preparation method of this natural drug composition and this natural drug composition are being used for preparing the purposes that reduces cancer chemotherapy dependency diarrhoea and treatment of cancer is played the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.The chemotherapy prescription with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan etc. in recent years, chemotherapy dependency diarrheal incidence rate improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, therefore also bring certain difficulty, to the important component part of chemotherapy dependency diarrheal effective treatment having become tumor patient wholistic therapy to CC.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and its etiology and pathogenesis mainly is the deficient of body negative and positive of qi and blood due to the spleen injury.Chinese medicine attracts people's attention for the research of having loose bowels, and present still unmatchful anticancer disease chemotherapy has the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Radix Sanguisorbae is the dry root of the rosaceous plant Radix Sanguisorbae or the Radix Sanguisorbae that comes into leaves, and the dysentery of stopping blooding is used for blood disorders, dysentery with red and white feces and watery diarrhea.Pericarpium Granati is the dry peel of Punicaceae plant Punica granatum L., and the energy relieving diarrhea with astringents cures mainly chronic diarrhea, chronic dysentery.In recent years, Radix Sanguisorbae and Pericarpium Granati antitumaous effect are confirmed, the inventor is through the research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, and proved that it has fabulous curative effect and can finish the present invention thus simultaneously to chemotherapeutic sensitivity cancer chemotherapy dependency diarrhoea.
Summary of the invention
A kind of natural drug composition provided by the invention, by Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin by weight in 2-8: the ratio of 1-2: 3-7: 5-8 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin is 8: 2: 7: 8.
Wherein, Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction can obtain by well known to a person skilled in the art that method is purified, and the natural drug of the present invention that these compositions that conventional method obtains or the commercial one-tenth of buying are grouped into all can obtain identical curative effect.
Preferably, Radix Sanguisorbae Pericarpium Granati water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
According to proportion compatibility is 1-4: 1 gets Radix Sanguisorbae, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure of vacuum drying oven, promptly.
The preparation method of radix sanguisorbae total saponin is in the above-mentioned natural drug composition:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaim ethanol extraction, this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are promptly.
Pericarpium Granati ethanol extraction preparation method is in the above-mentioned natural drug composition:
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
Pericarpium Granati tannin preparation method is in the above-mentioned natural drug composition:
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, and rotary evaporation removes and desolvates, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization promptly.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract
According to proportion compatibility is 1-4: 1 gets Radix Sanguisorbae, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure of vacuum drying oven, promptly.
(2) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaim ethanol extraction, this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are promptly.
(3) Pericarpium Granati ethanol extraction preparation
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
(4) Pericarpium Granati tannin preparation
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, and rotary evaporation removes and desolvates, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization promptly.
(5) choose Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin in proportion, uniform mixing, promptly.
The preparation method of above-mentioned natural drug composition, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen the inhibitory action of chemotherapeutics to tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined inhibitory action mouse interior tumor.Studies show that: this natural drug composition can obviously increase the inhibitory action of cyclophosphamide for tumor.
3, the inventor is originally with Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin make up, and by the consumption proportion between definite these components of a large amount of experiments, make said composition performance synergism, through experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-8: 1-2: 3-7: produced unexpected synergism during 5-8, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 8: 2: 7: 8 o'clock especially obvious, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and each component of Pericarpium Granati tannin all can obtain by conventional means, wherein component such as radix sanguisorbae total saponin also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract
According to proportion compatibility is 1-4: 1 gets Radix Sanguisorbae, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure of vacuum drying oven, promptly.
(2) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaim ethanol extraction, this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are promptly.
(3) Pericarpium Granati ethanol extraction preparation
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
(4) Pericarpium Granati tannin preparation
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, and rotary evaporation removes and desolvates, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization promptly.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Radix Sanguisorbae Pericarpium Granati water boiling concentration extract 32mg, radix sanguisorbae total saponin 8mg, Pericarpium Granati ethanol extraction 28mg and the Pericarpium Granati tannin 32mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, uniform mixing is filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Radix Sanguisorbae Pericarpium Granati water boiling concentration extract 32mg, radix sanguisorbae total saponin 8mg, Pericarpium Granati ethanol extraction 28mg and the Pericarpium Granati tannin 32mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, uniform mixing, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin is 8: 2: 7: 8.The inventor to above-mentioned each composition usage ratio at 2-8: 1-2: 3-7: the compositions that the multiple proportioning combination between 5-8 obtains has been carried out and last identical experiment, find that all the natural drug composition in this scope can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Radix Sanguisorbae, Pericarpium Granati is purchased in medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Radix Sanguisorbae Pericarpium Granati water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is radix sanguisorbae total saponin 10g.kg
-1(being equivalent to contain the crude drug amount), the D group is that Pericarpium Granati ethanol extraction and Pericarpium Granati tannin are pressed 7: 8 mixed 10g.kg
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, duplicates chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Radix Sanguisorbae Pericarpium Granati water boiling concentration extract group chemotherapy dependency diarrheal degree is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin by weight in 2-8: the ratio of 1-2: 3-7: 5-8 is formed
Wherein Radix Sanguisorbae Pericarpium Granati water boiling concentration preparation method of extract is:
According to proportion compatibility is 1-4: 1 gets Radix Sanguisorbae, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure of vacuum drying oven, promptly;
Wherein the preparation method of radix sanguisorbae total saponin is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaim ethanol extraction, this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are promptly;
Wherein the preparation method of Pericarpium Granati ethanol extraction is:
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction;
Wherein the preparation method of Pericarpium Granati tannin is:
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, and rotary evaporation removes and desolvates, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization promptly.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin is 8: 2: 7: 8.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Radix Sanguisorbae Pericarpium Granati water boiling concentration extract
According to proportion compatibility is 1-4: 1 gets Radix Sanguisorbae, Pericarpium Granati, 10 times of amount distilled water immersion 1h, heating decocts 1h, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure of vacuum drying oven, promptly;
(2) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaim ethanol extraction, this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are promptly;
(3) preparation of Pericarpium Granati ethanol extraction
Dry Pericarpium Granati is pulverized, and sieves, and 60% ethanol lixiviate 30min filters the back concentrating under reduced pressure, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction;
(4) preparation of Pericarpium Granati tannin
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone reflux, extract, again once merges twice extracting solution, and rotary evaporation removes and desolvates, and the thick liquid that obtains is placed pre-freeze 48h in the refrigerator, and lyophilization promptly;
(5) choose Radix Sanguisorbae Pericarpium Granati water boiling concentration extract, radix sanguisorbae total saponin, Pericarpium Granati ethanol extraction and Pericarpium Granati tannin uniform mixing in proportion, promptly.
4. the preparation method of natural drug composition as claimed in claim 3, it is characterized in that also comprising: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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| CN103655807A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition |
| CN103655804A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional chinese medicine composition |
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Application publication date: 20130731 |