CN103251660A - Traditional chinese medicine composition - Google Patents
Traditional chinese medicine composition Download PDFInfo
- Publication number
- CN103251660A CN103251660A CN2013101325218A CN201310132521A CN103251660A CN 103251660 A CN103251660 A CN 103251660A CN 2013101325218 A CN2013101325218 A CN 2013101325218A CN 201310132521 A CN201310132521 A CN 201310132521A CN 103251660 A CN103251660 A CN 103251660A
- Authority
- CN
- China
- Prior art keywords
- pericarpium granati
- galla chinensis
- extract
- preparation
- acetone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to a traditional Chinese medicine composition. The composition comprises the extract obtained by decocting Chinese gall and pomegranate rind with water and concentrating the product, acetone extract of Chinese gall, ethanol extract of pomegranate rind and tannins in pomegranate rind in a ratio of (2-4):(5-8):(3-4):(5-9). The composition has an excellent curative effect on cancer chemotherapy-induced diarrhea and simultaneously has a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition that is comprised of Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing the cancer chemotherapy induced diarrhea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is alone or unite with the toxicity such as all can cause that the patient suffers from diarrhoea, and diarrhoea is again Chemotherapy-induced Diarrhea behind the chemotherapy of tumors.In recent years emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and its etiology and pathogenesis mainly is the deficient of body negative and positive of qi and blood due to the spleen injury.Chinese medicine attracts people's attention for the research of having loose bowels, and there is no at present the Chinese patent medicine that the antagonism cancer chemotherapy has alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating the cancer chemotherapy induced diarrhea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Galla Chinensis can be astringed the lung, and astringing intestine to stop diarrhea cures mainly the chronic dysentery chronic diarrhea.Pericarpium Granati is the dry peel of Punicaceae plant Punica granatum L., and the energy relieving diarrhea with astringents cures mainly chronic diarrhea, chronic dysentery.In recent years, Galla Chinensis and Pericarpium Granati antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained at last optimal drug ratio compositions of the present invention, and proved that it has fabulous curative effect and can simultaneously to chemotherapeutic sensitivity, finish thus the present invention the cancer chemotherapy induced diarrhea.
Summary of the invention
A kind of natural drug composition provided by the invention, by Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L by weight in 2-4: the ratio of 5-8: 3-4: 5-9 forms.
Above-mentioned natural drug composition, wherein the usage ratio of Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L is 4: 8: 4: 9.
Wherein, Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction can be by well known to a person skilled in the art the method acquisition of purifying, and the natural drug of the present invention that these compositions that conventional method obtains or the commercial one-tenth of buying are grouped into all can obtain identical curative effect.
Preferably, the preparation method of Galla Chinensis Pericarpium Granati water boiling concentration extract is in the above-mentioned natural drug composition:
Be 1-4 according to proportion compatibility: 1 gets Galla Chinensis, Pericarpium Granati, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, and get final product.
The preparation method of Galla Chinensis acetone extract is in the above-mentioned natural drug composition:
Galla Chinensis is pulverized, and adds acetone, and the water-bath lixiviate is filtered, and filtrate drips 1% CaCl
2To no longer producing precipitation, the centrifugal 15min of 10000r/min gets precipitation, drips 0.01mol/L HCl to fully dissolving of precipitation, the bag filter of packing into, and dialysis, the rotary evaporation postlyophilization, and get final product.
Pericarpium Granati ethanol extraction preparation method is in the above-mentioned natural drug composition:
Dry Pericarpium Granati is pulverized, sieves, and 60% alcohol steep 30min, concentrating under reduced pressure after filtering, the extractum lyophilization, and get final product.
The Tannin in Punica granatum L preparation method is in the above-mentioned natural drug composition:
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone again reflux, extract, once merges twice extracting solution, rotary evaporation desolventizing, the thick liquid that obtains, pre-freeze 48h in the placement refrigerator, lyophilization and get final product.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Galla Chinensis Pericarpium Granati water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Galla Chinensis, Pericarpium Granati, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, and get final product.
(2) preparation of Galla Chinensis acetone extract
Galla Chinensis is pulverized, and adds acetone, and the water-bath lixiviate is filtered, and filtrate drips 1% CaCl
2To no longer producing precipitation, the centrifugal 15min of 10000r/min gets precipitation, drips 0.01mol/L HCl to fully dissolving of precipitation, the bag filter of packing into, and dialysis, the rotary evaporation postlyophilization, and get final product.
(3) Pericarpium Granati ethanol extraction preparation
Dry Pericarpium Granati is pulverized, sieves, and 60% alcohol steep 30min, concentrating under reduced pressure after filtering, the extractum lyophilization, and get final product.
(4) Tannin in Punica granatum L preparation
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone again reflux, extract, once merges twice extracting solution, rotary evaporation desolventizing, the thick liquid that obtains, pre-freeze 48h in the placement refrigerator, lyophilization and get final product.
(5) choose in proportion Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L, evenly mix, and get final product.
The preparation method of above-mentioned natural drug composition also comprises: the product that step (5) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of natural drug composition of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes studies show that of diarrhea of mouse model at irinotecan: the diarrhoea reaction for the irinotecan induced mice has obvious antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor is originally with Galla Chinensis Pericarpium Granati water boiling concentration extract, the Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L make up, and determine consumption proportion between these components by great many of experiments, make said composition performance synergism, through experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-4: 5-8: 3-4: produce synergism during 5-9, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 4: 8: 4: 9 o'clock especially obvious, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't obtain this proportioning and optimum amount ratio by disclosed content.
4, this natural drug composition Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and each component of Tannin in Punica granatum L all can obtain by conventional means, Tannin in Punica granatum L also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Galla Chinensis Pericarpium Granati water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Galla Chinensis, Pericarpium Granati, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, and get final product.
(2) preparation of Galla Chinensis acetone extract
Galla Chinensis is pulverized, and adds acetone, and the water-bath lixiviate is filtered, and filtrate drips 1% CaCl
2To no longer producing precipitation, the centrifugal 15min of 10000r/min gets precipitation, drips 0.01mol/L HCl to fully dissolving of precipitation, the bag filter of packing into, and dialysis, the rotary evaporation postlyophilization, and get final product.
(3) Pericarpium Granati ethanol extraction preparation
Dry Pericarpium Granati is pulverized, sieve, 60% alcohol steep 30min, concentrating under reduced pressure after filtering, after the extractum lyophilization light yellow to the yellowish-brown powder, be the Pericarpium Granati ethanol extraction.
(4) Tannin in Punica granatum L preparation
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone again reflux, extract, once merges twice extracting solution, rotary evaporation desolventizing, the thick liquid that obtains, pre-freeze 48h in the placement refrigerator, lyophilization and get final product.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing respectively Galla Chinensis Pericarpium Granati water boiling concentration extract 16mg, Galla Chinensis acetone extract 32mg, Pericarpium Granati ethanol extraction 16mg and the Tannin in Punica granatum L 24mg of above-mentioned steps preparation, take by weighing simultaneously starch 395mg, magnesium stearate 5mg, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing respectively Galla Chinensis Pericarpium Granati water boiling concentration extract 16mg, Galla Chinensis acetone extract 32mg, Pericarpium Granati ethanol extraction 16mg and the Tannin in Punica granatum L 36mg of above-mentioned steps preparation, take by weighing simultaneously starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L is 4: 8: 4: 9.The inventor to above-mentioned each composition usage ratio at 2-4: 5-8: 3-4: the compositions that the multiple proportioning combination in the time of between the 5-9 obtains has been carried out and upper identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and the treatment of cancer is had potentiation the cancer chemotherapy induced diarrhea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Galla Chinensis, Pericarpium Granati is purchased from medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Galla Chinensis Pericarpium Granati water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Galla Chinensis acetone extract 10g.kg
-1(being equivalent to contain the crude drug amount), D group Pericarpium Granati ethanol extraction mixes 10g.kg with Tannin in Punica granatum L in 4: 9 ratios
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies the Chemotherapy-induced Diarrhea model at every day 1 time.B, C, D, E group with corresponding extract gavage 8 days, places metabolic cage with mice continuously, spreads white filter paper at the bottom of the cage observing the stool situation, and observes mice crissum situation and Mus tail fecal pollution situation, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wet and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, the degree of Galla Chinensis Pericarpium Granati water boiling concentration extract group Chemotherapy-induced Diarrhea is lower than model group, and natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).The inoculation next day random packet, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus gavage 0.5ml gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives the 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(such as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. one kind is reduced the cancer chemotherapy induced diarrhea and the treatment cancer is played the natural drug composition of potentiation, it is characterized in that said composition by Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L by weight in 2-4: the ratio of 5-8: 3-4: 5-9 forms
Wherein the preparation method of Galla Chinensis Pericarpium Granati water boiling concentration extract is:
Be 1-4 according to proportion compatibility: 1 gets Galla Chinensis, Pericarpium Granati, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, and get final product;
Wherein the preparation method of Galla Chinensis acetone extract is:
Galla Chinensis is pulverized, and adds acetone, and the water-bath lixiviate is filtered, and filtrate drips 1% CaCl
2To no longer producing precipitation, the centrifugal 15min of 10000r/min gets precipitation, drips 0.01mol/L HCl to fully dissolving of precipitation, the bag filter of packing into, and dialysis, the rotary evaporation postlyophilization, and get final product;
Wherein the preparation method of Pericarpium Granati ethanol extraction is:
Dry Pericarpium Granati is pulverized, sieves, and 60% alcohol steep 30min, concentrating under reduced pressure after filtering, the extractum lyophilization, and get final product;
Wherein the preparation method of Tannin in Punica granatum L is:
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone again reflux, extract, once merges twice extracting solution, rotary evaporation desolventizing, the thick liquid that obtains, pre-freeze 48h in the placement refrigerator, lyophilization and get final product.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction and Tannin in Punica granatum L is 4: 8: 4: 9.
3. such as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Galla Chinensis Pericarpium Granati water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Galla Chinensis, Pericarpium Granati, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, and get final product;
(2) preparation of Galla Chinensis acetone extract
Galla Chinensis is pulverized, and adds acetone, and the water-bath lixiviate is filtered, and filtrate drips 1% CaCl
2To no longer producing precipitation, the centrifugal 15min of 10000r/min gets precipitation, drips 0.01mol/L HCl to fully dissolving of precipitation, the bag filter of packing into, and dialysis, the rotary evaporation postlyophilization, and get final product;
(3) preparation of Pericarpium Granati ethanol extraction
Dry Pericarpium Granati is pulverized, sieves, and 60% alcohol steep 30min, concentrating under reduced pressure after filtering, the extractum lyophilization, and get final product;
(4) preparation of Tannin in Punica granatum L
Dry Pericarpium Granati is pulverized, and sieves, and 50% acetone reflux, extract, 2h filters, and filtering residue 50% acetone again reflux, extract, once merges twice extracting solution, rotary evaporation desolventizing, the thick liquid that obtains, pre-freeze 48h in the placement refrigerator, lyophilization and get final product;
(5) choosing in proportion Galla Chinensis Pericarpium Granati water boiling concentration extract, Galla Chinensis acetone extract, Pericarpium Granati ethanol extraction evenly mixes with Tannin in Punica granatum L, and get final product.
4. the preparation method of natural drug composition as claimed in claim 3 characterized by further comprising: the product that step (5) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
5. such as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy induced diarrhea and the treatment cancer is played potentiation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101325218A CN103251660A (en) | 2013-04-08 | 2013-04-08 | Traditional chinese medicine composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101325218A CN103251660A (en) | 2013-04-08 | 2013-04-08 | Traditional chinese medicine composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103251660A true CN103251660A (en) | 2013-08-21 |
Family
ID=48956093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101325218A Pending CN103251660A (en) | 2013-04-08 | 2013-04-08 | Traditional chinese medicine composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103251660A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655647A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101020014A (en) * | 2006-12-15 | 2007-08-22 | 陆成玉 | Specific functional food for treating diarrhea |
| CN101972276A (en) * | 2010-10-20 | 2011-02-16 | 华南理工大学 | Transdermal drug delivery system for treating infantile diarrhea and preparation method thereof |
-
2013
- 2013-04-08 CN CN2013101325218A patent/CN103251660A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101020014A (en) * | 2006-12-15 | 2007-08-22 | 陆成玉 | Specific functional food for treating diarrhea |
| CN101972276A (en) * | 2010-10-20 | 2011-02-16 | 华南理工大学 | Transdermal drug delivery system for treating infantile diarrhea and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 帅益武等: "五倍子中鞣质的提取、分离纯化研究", 《食品科技》, 31 December 2007 (2007-12-31), pages 125 - 128 * |
| 李昊: "久泻辩治心得", 《江苏中医》, vol. 20, no. 8, 31 August 1999 (1999-08-31) * |
| 汝绍刚等: "提取方法和溶剂对石榴皮中抑菌成分提取的影响", 《化学与生物工程》, vol. 28, no. 12, 31 December 2001 (2001-12-31) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655647A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103316112A (en) | Natural pharmaceutical composition | |
| CN103301377A (en) | Natural pharmaceutical composition | |
| CN103223085A (en) | Chinese medicinal extract | |
| CN103223036A (en) | Traditional Chinese medicinal composition | |
| CN103316115A (en) | Traditional Chinese medicinal composition and preparation method thereof | |
| CN103251771A (en) | Traditional chinese medicine composition | |
| CN103316190A (en) | Natural pharmaceutical composition | |
| CN103223004A (en) | Chinese medicinal composition | |
| CN103340929A (en) | Natural drug extract | |
| CN103263475A (en) | Traditional chinese medicine composition | |
| CN103349713A (en) | Natural pharmaceutical extractive, as well as preparation method and use thereof | |
| CN103340970A (en) | Traditional chinese medicine composition | |
| CN103239529A (en) | Traditional chinese medicine composition | |
| CN103230496A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN103251660A (en) | Traditional chinese medicine composition | |
| CN103356814A (en) | Chinese medicinal composition, its preparation method and application | |
| CN103263499A (en) | Traditional chinese medicine extract | |
| CN103340934A (en) | Natural medicament composition | |
| CN103230559A (en) | Traditional Chinese medicine composition and preparation method thereof | |
| CN103330730A (en) | Natural medicine composition and preparation method thereof | |
| CN103393811A (en) | Traditional Chinese medicine composition and preparation method thereof | |
| CN103330752A (en) | Natural medicine composition, preparation method and purpose of natural medicine composition | |
| CN103316189A (en) | Natural pharmaceutical composition | |
| CN103356729A (en) | Chinese medicinal composition | |
| CN103330745A (en) | Natural pharmaceutical composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130821 |