CN103316190A - Natural pharmaceutical composition - Google Patents
Natural pharmaceutical composition Download PDFInfo
- Publication number
- CN103316190A CN103316190A CN201310274187XA CN201310274187A CN103316190A CN 103316190 A CN103316190 A CN 103316190A CN 201310274187X A CN201310274187X A CN 201310274187XA CN 201310274187 A CN201310274187 A CN 201310274187A CN 103316190 A CN103316190 A CN 103316190A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- rhizoma dioscoreae
- fructus chebulae
- preparation
- supernatant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a natural pharmaceutical composition. The natural pharmaceutical composition comprises a myrobalan and Chinese yam water-decocted concentration extract, a myrobalan 70% ethanol extract, a Chinese yam ethanol extract and Chinese yam polysaccharide in a proportion of (2-5):(2-8):(5-6):(4-6). The composition has extremely good curative effect on cancer chemotherapy associated diarrhea and synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition that is comprised of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing the cancer chemotherapy induced diarrhea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is the cell toxicant antitumor drug, and no matter alone or unite with the toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea is again Chemotherapy-induced Diarrhea behind the chemotherapy of tumors.In recent years emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but there is no at present the Chinese patent medicine that the antagonism cancer chemotherapy has alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating the cancer chemotherapy induced diarrhea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
The Fructus Chebulae calls tender Fructus Chebulae, chebule, scolds multitude, scolds pears, son with the wind.Sour in the mouth is puckery; Property is put down, and returns lung, large intestine channel; The relieving diarrhea with astringents effect is arranged, and is to control chronic diarrhea; Chronic dysentery; The common medicine of proctoptosis.Rhizoma Dioscoreae is perennial voluble herb, Chinese medicine, vegetable dual-purpose, and motherland's medical science thinks, the multiple efficacies such as Rhizoma Dioscoreae has spleen invigorating, tonifying the lung, reinforces the kidney, benefit essence have therapeutical effect to diarrhea due to hypofunction of the spleen.In recent years, Fructus Chebulae and Rhizoma Dioscoreae antitumaous effect are confirmed, especially Fructus Chebulae's 70% ethanol extraction and Chinese yam polysaccharide, the inventor is through research and the experiment of each a large amount of formula proportion, obtained at last optimal drug ratio compositions of the present invention, be the compositions of novelty, and proved that it has fabulous curative effect and can play simultaneously the effect that suppresses tumor the cancer chemotherapy induced diarrhea, has finished the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention is made of in the ratio of 2-5:2-8:5-6:4-6 by weight Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide.
Above-mentioned natural drug composition, wherein the usage ratio of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 5:8:6:6.
Wherein, Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, the preparation method of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract is in the above-mentioned natural drug composition:
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Chebulae, Rhizoma Dioscoreae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get the brown color powder, is Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract.
The preparation method of Fructus Chebulae's 70% ethanol extraction is in the above-mentioned natural drug composition:
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, and get final product.
Rhizoma Dioscoreae ethanol extraction preparation method is in the above-mentioned natural drug composition:
Get dried Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h collect extracting solution, and are centrifugal, collect supernatant, with supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product.
The Chinese yam polysaccharide preparation method is in the above-mentioned natural drug composition:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Chebulae, Rhizoma Dioscoreae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get the brown color powder, is Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract.
(2) preparation of Fructus Chebulae's 70% ethanol extraction
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, and get final product.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get dried Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h collect extracting solution, and are centrifugal, collect supernatant, with supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
(5) choose in proportion Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, evenly mix, and get final product.
The preparation method of above-mentioned natural drug composition, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
The beneficial effect of natural drug composition of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes studies show that of diarrhea of mouse model at irinotecan: the diarrhoea reaction for the irinotecan induced mice has obvious antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide originally, and determine consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our repeatedly experimentation, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-5:2-8:5-6:4-6, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 5:8:6:6, this is that the inventor pays a large amount of creative works by keeping punching and obtains on the paper basis of delivering before, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and each component of Chinese yam polysaccharide all can obtain by conventional means, some of them component for example Fructus Chebulae's 70% ethanol extraction and Chinese yam polysaccharide also can be buied by commerce, and such compositions itself has and obtains convenient, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Fructus Chebulae, Rhizoma Dioscoreae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get the brown color powder, is Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract.
(2) preparation of Fructus Chebulae's 70% ethanol extraction
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, and get final product.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get dried Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h collect extracting solution, and are centrifugal, collect supernatant, with supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing respectively Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract 20mg, Fructus Chebulae 70% ethanol extraction 32mg, Rhizoma Dioscoreae ethanol extraction 24mg and the Chinese yam polysaccharide 24mg of above-mentioned steps preparation, take by weighing simultaneously starch 395mg, magnesium stearate 5mg, evenly mix, filled capsules is made the capsule of each pure heavy 500mg.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing respectively Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract 20mg, Fructus Chebulae 70% ethanol extraction 32mg, Rhizoma Dioscoreae ethanol extraction 24mg and the Chinese yam polysaccharide 24mg of above-mentioned steps preparation, take by weighing simultaneously starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, tabletting is made the tablet of every pure heavy 500mg.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 5:8:6:6.Inventor's compositions that the multiple proportioning combination between 2-5:2-8:5-6:4-6 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and the treatment of cancer is had potentiation the cancer chemotherapy induced diarrhea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Fructus Chebulae, Rhizoma Dioscoreae is purchased from medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Fructus Chebulae 70% ethanol extraction 10g.kg
-1(being equivalent to contain the crude drug amount), the D group is mixed 10g.kg with Chinese yam polysaccharide in the 6:6 ratio for the Rhizoma Dioscoreae ethanol extraction
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies the Chemotherapy-induced Diarrhea model at every day 1 time.B, C, D, E group with corresponding extract gavage 8 days, places metabolic cage with mice continuously, spreads white filter paper at the bottom of the cage observing the stool situation, and observes mice crissum situation and Mus tail fecal pollution situation, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wet and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
(1) to mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 6 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).The inoculation next day random packet, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus gavage 0.5 ml gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group inhibitory action.(such as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 31.5 |
| Compositions is low+cyclophosphamide | 38.7 |
| Height+cyclophosphamide | 46.1 |
Claims (5)
1. one kind is reduced the cancer chemotherapy induced diarrhea and the treatment cancer is played the natural drug composition of potentiation, it is characterized in that said composition is made of in the ratio of 2-5:2-8:5-6:4-6 by weight Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide
Wherein the preparation method of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract is:
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Rhizoma Dioscoreae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get the brown color powder, is Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract;
Wherein the preparation method of Fructus Chebulae's 70% ethanol extraction is:
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, and get final product;
Wherein the preparation method of Rhizoma Dioscoreae ethanol extraction is:
Get dried Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h collect extracting solution, and are centrifugal, collect supernatant, with supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product;
Wherein the preparation method of Chinese yam polysaccharide is:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 5:8:6:6.
3. such as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Rhizoma Dioscoreae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get the brown color powder, is Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract;
(2) preparation of Fructus Chebulae's 70% ethanol extraction
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, and drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, and get final product;
(3) preparation of Rhizoma Dioscoreae ethanol extraction
Get dried Rhizoma Dioscoreae powder, with 95% ethanol at 70 ℃ of reflux, extract, 3 times, each 4 h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3 h collect extracting solution, and are centrifugal, collect supernatant, with supernatant concentration, add the ethanol of 4 times of volumes, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, use respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product;
(4) preparation of Chinese yam polysaccharide
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeatedly, until without white intermediate layer precipitation. collect supernatant concentration, use respectively tap water and distill water dialysis, add 4 times of volume ethanol, place a whole night for 4 ℃, centrifugal, collecting precipitation, use respectively ethanol, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide;
(5) choosing in proportion Fructus Chebulae Rhizoma Dioscoreae water boiling concentration extract, Fructus Chebulae's 70% ethanol extraction, Rhizoma Dioscoreae ethanol extraction evenly mixes with Chinese yam polysaccharide, and get final product.
4. the preparation method of natural drug composition as claimed in claim 3, characterized by further comprising: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. such as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy induced diarrhea and the treatment cancer is played potentiation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310274187XA CN103316190A (en) | 2013-07-02 | 2013-07-02 | Natural pharmaceutical composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310274187XA CN103316190A (en) | 2013-07-02 | 2013-07-02 | Natural pharmaceutical composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103316190A true CN103316190A (en) | 2013-09-25 |
Family
ID=49185423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310274187XA Pending CN103316190A (en) | 2013-07-02 | 2013-07-02 | Natural pharmaceutical composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103316190A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655647A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition |
| CN108498578A (en) * | 2018-07-09 | 2018-09-07 | 黄振成 | It is a kind of to treat uremic drug and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102836314A (en) * | 2011-06-21 | 2012-12-26 | 吴同科 | Traditional Chinese medicine antidiarrheal powder for treating ulcerative colitis |
-
2013
- 2013-07-02 CN CN201310274187XA patent/CN103316190A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102836314A (en) * | 2011-06-21 | 2012-12-26 | 吴同科 | Traditional Chinese medicine antidiarrheal powder for treating ulcerative colitis |
Non-Patent Citations (3)
| Title |
|---|
| VAIBHAV AHER ET AL.: "Immunomodulatory Activity of Alcohol Extract of Terminalia chebula Retz Combretaceae", 《TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH》 * |
| 尚晓娅等: "山药多糖的制备及其体外抗氧化活性", 《化学研究》 * |
| 摘自《郑侨医案选》: "单验方选载", 《贵阳中医学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655647A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition |
| CN108498578A (en) * | 2018-07-09 | 2018-09-07 | 黄振成 | It is a kind of to treat uremic drug and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103182016A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103316112A (en) | Natural pharmaceutical composition | |
| CN103301377A (en) | Natural pharmaceutical composition | |
| CN103182006A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103316190A (en) | Natural pharmaceutical composition | |
| CN103202886B (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103316115A (en) | Traditional Chinese medicinal composition and preparation method thereof | |
| CN103223082A (en) | Extract product of traditional Chinese medicine | |
| CN103251771A (en) | Traditional chinese medicine composition | |
| CN103385922A (en) | Natural pharmaceutical composition and preparation method | |
| CN103356769A (en) | Natural drug extract, preparation method and application thereof | |
| CN103223085A (en) | Chinese medicinal extract | |
| CN103223040A (en) | Traditional chinese medicine composition | |
| CN103316191A (en) | Natural medicine extract | |
| CN103301293A (en) | Natural medicine composition | |
| CN103356768A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103349681A (en) | Natural medicine composition | |
| CN103301225A (en) | Natural pharmaceutical composition | |
| CN103316189A (en) | Natural pharmaceutical composition | |
| CN103251660A (en) | Traditional chinese medicine composition | |
| CN103393790A (en) | Natural medicine extract as well as preparation method and applications thereof | |
| CN103263456B (en) | Traditional chinese medicine composition | |
| CN103356814A (en) | Chinese medicinal composition, its preparation method and application | |
| CN103330807A (en) | Natural drug composite, and preparation method and purpose thereof | |
| CN103263463A (en) | Traditional chinese medicine composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130925 |