CN103356769A - Natural drug extract, preparation method and application thereof - Google Patents
Natural drug extract, preparation method and application thereof Download PDFInfo
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- CN103356769A CN103356769A CN2013102909368A CN201310290936A CN103356769A CN 103356769 A CN103356769 A CN 103356769A CN 2013102909368 A CN2013102909368 A CN 2013102909368A CN 201310290936 A CN201310290936 A CN 201310290936A CN 103356769 A CN103356769 A CN 103356769A
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Abstract
The invention relates to a natural drug extract, a preparation method and application thereof. The natural drug extract is prepared from a water-decocted and concentrated extract of smoked plum and syzygium aromaticum, an ethanol extract of smoked plum, an ethanol extract of syzygium aromaticum and eugenol according to the ratio of (2-5): (3-5): (4-7): (5-8). The composition has a good curative effect on related diarrhea of cancer chemotherapy and a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug extract, relate more specifically to a kind of natural drug extract that is comprised of Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol, the preparation method of this natural drug extract and this natural drug extract are for the preparation of reducing the cancer chemotherapy induced diarrhea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is no matter alone or unite with the toxicity such as all can cause that the patient suffers from diarrhoea, and diarrhoea is again Chemotherapy-induced Diarrhea behind the chemotherapy of tumors.In recent years emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but there is no at present the Chinese patent medicine that the antagonism cancer chemotherapy has alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating the cancer chemotherapy induced diarrhea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Fructus Mume is that the dry almost ripe fruit by rose department plant plum processes, and can astringe the lung astringing intestine to stop diarrhea.Be used for chronic diarrhea; Dysentery; The diseases such as vomiting.Flos Caryophylli is alabastrum and the fruit of Myrtaceae syzygium species plant Flos Caryophylli Eugenia caryophyllata Thunb.The energy warming the stomach, the kidney warming.Be used for the treatment of singultus, vomiting and diarrhoea, cure mainly dysentery.In recent years, Fructus Mume and Flos Caryophylli antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained at last optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can simultaneously to chemotherapeutic sensitivity, finish thus the present invention the cancer chemotherapy induced diarrhea.
Summary of the invention
A kind of natural drug extract provided by the invention is made of in the ratio of 2-5:3-5:4-7:5-8 by weight Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol.
Above-mentioned natural drug extract, wherein the usage ratio of Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol is 5:5:7:8.
Wherein, Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, the preparation method of Fructus Mume Flos Caryophylli decocting concentrated extract is in the above-mentioned natural drug extract:
Be to get Fructus Mume, Flos Caryophylli at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product.
The preparation method of Fructus Mume ethanol extraction is in the above-mentioned natural drug extract:
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, and filtrate merges, centrifugal 15 minutes of 3000rpm, and separation of supernatant, water bath method is concentrated, and drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
Flos Caryophylli ethanol extraction preparation method is in the above-mentioned natural drug extract:
Get Flos Caryophylli, drying is pulverized, and sieves, and soak with ethanol is filtered, and residue continues to soak, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, ethyl acetate extraction, drying under reduced pressure becomes dry extract, pulverizes, and sieves, and get final product.
The eugenol preparation method is in the above-mentioned natural drug extract:
Flos Caryophylli is pulverized, with the little lower backflow 30min that boils of 95% ethanol, filter, filtrate is fixed with 5% sodium hydroxide, filters, and steams except ethanol, uses the 25mL extracted with diethyl ether 3 times at every turn; The water layer hcl acidifying is transferred pH value of solution=5, uses extracted with diethyl ether 3 times, and combining extraction liquid is used anhydrous sodium sulfate drying, steams except ether, gets the pale yellow oily liquid body, is eugenol.
The present invention also provides the preferred preparation method of this natural drug extract, comprising:
(1) preparation of Fructus Mume Flos Caryophylli decocting concentrated extract
Be to get Fructus Mume, Flos Caryophylli at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product.
(2) preparation of Fructus Mume ethanol extraction
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, and filtrate merges, centrifugal 15 minutes of 3000rpm, and separation of supernatant, water bath method is concentrated, and drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
(3) Flos Caryophylli ethanol extraction preparation
Get Flos Caryophylli, drying is pulverized, and sieves, and soak with ethanol is filtered, and residue continues to soak, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, ethyl acetate extraction, drying under reduced pressure becomes dry extract, pulverizes, and sieves, and get final product.
(4) eugenol preparation
Flos Caryophylli is pulverized, with the little lower backflow 30min that boils of 95% ethanol, filter, filtrate is fixed with 5% sodium hydroxide, filters, and steams except ethanol, uses the 25mL extracted with diethyl ether 3 times at every turn; The water layer hcl acidifying is transferred pH value of solution=5, uses extracted with diethyl ether 3 times, and combining extraction liquid is used anhydrous sodium sulfate drying, steams except ether, gets the pale yellow oily liquid body, is eugenol.
(5) choose in proportion Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol, evenly mix, and get final product.
The preparation method of above-mentioned natural drug extract also comprises: the product that step (5) makes is further made capsule or the tablet that contains natural drug extract 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of natural drug extract of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, natural drug extract of the present invention causes studies show that of diarrhea of mouse model at irinotecan: the diarrhoea reaction for the irinotecan induced mice has obvious antagonism.
2, proved that this natural drug extract can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug extract and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug extract can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol originally, and determine consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our repeatedly experimentation, ratio between each component of natural drug extract of the present invention has produced unexpected synergism when 2-5:3-5:4-7:5-8, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 5:5:7:8, this is that the inventor pays a large amount of creative works by keeping punching and obtains on the paper basis of delivering before, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug extract Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and each component of eugenol all can obtain by conventional means, some of them component for example eugenol also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug extract of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Mume Flos Caryophylli decocting concentrated extract
Be to get Fructus Mume, Flos Caryophylli at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product.
(2) preparation of Fructus Mume ethanol extraction
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, and filtrate merges, centrifugal 15 minutes of 3000rpm, and separation of supernatant, water bath method is concentrated, and drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
(3) Flos Caryophylli ethanol extraction preparation
Get Flos Caryophylli, drying is pulverized, and sieves, and soak with ethanol is filtered, and residue continues to soak, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, ethyl acetate extraction, drying under reduced pressure becomes dry extract, pulverizes, and sieves, and get final product.
(4) eugenol preparation
Flos Caryophylli is pulverized, with the little lower backflow 30min that boils of 95% ethanol, filter, filtrate is fixed (pH=9～10) with 5% sodium hydroxide, filters, and steams except ethanol, uses the 25mL extracted with diethyl ether 3 times at every turn; The water layer hcl acidifying is transferred pH value of solution=5, uses extracted with diethyl ether 3 times, and combining extraction liquid is used anhydrous sodium sulfate drying, steams except ether, gets the pale yellow oily liquid body, is eugenol.
Second step: the preparation of preparation
(1) preparation of natural drug extract capsule preparation of the present invention
Take by weighing respectively Fructus Mume Flos Caryophylli decocting concentrated extract 20mg, Fructus Mume ethanol extraction 20mg, Flos Caryophylli ethanol extraction 28mg and the eugenol 32mg of above-mentioned steps preparation, take by weighing simultaneously starch 395mg, magnesium stearate 5mg, evenly mix, be filled to capsule.
(2) preparation of natural drug extract tablet of the present invention
Take by weighing respectively Fructus Mume Flos Caryophylli decocting concentrated extract 20mg, Fructus Mume ethanol extraction 20mg, Flos Caryophylli ethanol extraction 28mg and the eugenol 32mg of above-mentioned steps preparation, take by weighing simultaneously starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol is 5:5:7:8.Inventor's compositions that the multiple proportioning combination between 2-5:3-5:4-7:5-8 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the natural drug extract in this amount ranges can obtain good synergism, can play good effect and the treatment of cancer is had potentiation the cancer chemotherapy induced diarrhea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug extract for treating Chemotherapy-induced Diarrhea effect
1, test material
(1) Fructus Mume, Flos Caryophylli is purchased from medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Fructus Mume Flos Caryophylli decocting concentrated extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Fructus Mume ethanol extraction 10g.kg
-1(being equivalent to contain the crude drug amount), D group Flos Caryophylli ethanol extraction mixes 10g.kg with eugenol in the 7:8 ratio
-1(being equivalent to contain the crude drug amount), the E group is natural drug extract 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies the Chemotherapy-induced Diarrhea model at every day 1 time.B, C, D, E group with corresponding extract gavage 8 days, places metabolic cage with mice continuously, spreads white filter paper at the bottom of the cage observing the stool situation, and observes mice crissum situation and Mus tail fecal pollution situation, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wet and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, natural drug extract group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug extract antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).The inoculation next day random packet, experimental group natural drug extract of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus gavage 0.5 ml gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug extract of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with effect of high dosage.(such as table 2)
Table 2 natural drug extract of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 32.5 |
| Compositions is low+cyclophosphamide | 41.2 |
| Height+cyclophosphamide | 55.6 |
Claims (5)
1. one kind is reduced the cancer chemotherapy induced diarrhea and the treatment cancer is played the natural drug extract of potentiation, it is characterized in that said composition is made of in the ratio of 2-5:3-5:4-7:5-8 by weight Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol
Wherein the preparation method of Fructus Mume Flos Caryophylli decocting concentrated extract is:
Be to get Fructus Mume, Flos Caryophylli at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product;
Wherein the preparation method of Fructus Mume ethanol extraction is:
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, and filtrate merges, centrifugal 15 minutes of 3000rpm, and separation of supernatant, water bath method is concentrated, and drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product;
Wherein the preparation method of Flos Caryophylli ethanol extraction is:
Get Flos Caryophylli, drying is pulverized, and sieves, and soak with ethanol is filtered, and residue continues to soak, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, ethyl acetate extraction, drying under reduced pressure becomes dry extract, pulverizes, and sieves, and get final product;
Wherein the preparation method of eugenol is:
Flos Caryophylli is pulverized, with the little lower backflow 30min that boils of 95% ethanol, filter, filtrate is fixed with 5% sodium hydroxide, filters, and steams except ethanol, uses the 25mL extracted with diethyl ether 3 times at every turn; The water layer hcl acidifying is transferred pH value of solution=5, uses extracted with diethyl ether 3 times, and combining extraction liquid is used anhydrous sodium sulfate drying, steams except ether, gets the pale yellow oily liquid body, is eugenol.
2. natural drug extract as claimed in claim 1 is characterized in that wherein the usage ratio of Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction and eugenol is 5:5:7:8.
3. such as the preparation method of the natural drug extract of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Fructus Mume Flos Caryophylli decocting concentrated extract
Be to get Fructus Mume, Flos Caryophylli at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product;
(2) preparation of Fructus Mume ethanol extraction
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, and filtrate merges, centrifugal 15 minutes of 3000rpm, and separation of supernatant, water bath method is concentrated, and drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product;
(3) preparation of Flos Caryophylli ethanol extraction
Get Flos Caryophylli, drying is pulverized, and sieves, and soak with ethanol is filtered, and residue continues to soak, twice filtrate is merged, and centrifugal 15 minutes of 3000rpm, separation of supernatant, ethanol is removed in distillation, ethyl acetate extraction, drying under reduced pressure becomes dry extract, pulverizes, and sieves, and get final product;
(4) preparation of eugenol
Flos Caryophylli is pulverized, with the little lower backflow 30min that boils of 95% ethanol, filter, filtrate is fixed with 5% sodium hydroxide, filters, and steams except ethanol, uses the 25mL extracted with diethyl ether 3 times at every turn; The water layer hcl acidifying is transferred pH value of solution=5, uses extracted with diethyl ether 3 times, and combining extraction liquid is used anhydrous sodium sulfate drying, steams except ether, gets the pale yellow oily liquid body, is eugenol;
(5) choosing in proportion Fructus Mume Flos Caryophylli decocting concentrated extract, Fructus Mume ethanol extraction, Flos Caryophylli ethanol extraction evenly mixes with eugenol, and get final product.
4. the preparation method of natural drug extract as claimed in claim 3 characterized by further comprising: the product that step (5) makes is further made capsule or the tablet that contains natural drug extract 100mg or 200mg specification by the pharmacy means of routine.
5. such as the purposes of each described natural drug extract of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy induced diarrhea and the treatment cancer is played potentiation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655720A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition and preparation method thereof |
| CN103655933A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition as well as preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091315A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Ginger and dark plum fruit composition and preparation method thereof, and application of ginger and dark plum fruit composition in preparation of attenuation and synergy medicaments for radiotherapy and chemotherapy of cancers |
| CN102145158A (en) * | 2011-03-31 | 2011-08-10 | 青岛大学 | Ginger and clove composition, preparation method of ginger and clove composition and use of ginger and clove composition for preparing toxicity-reducing efficacy-improving medicine in cancer radiotherapy and chemotherapy |
| CN102319412A (en) * | 2011-09-27 | 2012-01-18 | 解连新 | Traditional Chinese medicine used for treating diarrhea |
-
2013
- 2013-07-11 CN CN2013102909368A patent/CN103356769A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091315A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Ginger and dark plum fruit composition and preparation method thereof, and application of ginger and dark plum fruit composition in preparation of attenuation and synergy medicaments for radiotherapy and chemotherapy of cancers |
| CN102145158A (en) * | 2011-03-31 | 2011-08-10 | 青岛大学 | Ginger and clove composition, preparation method of ginger and clove composition and use of ginger and clove composition for preparing toxicity-reducing efficacy-improving medicine in cancer radiotherapy and chemotherapy |
| CN102319412A (en) * | 2011-09-27 | 2012-01-18 | 解连新 | Traditional Chinese medicine used for treating diarrhea |
Non-Patent Citations (2)
| Title |
|---|
| 王晓玲: "中西医结合治疗婴幼儿迁延性腹泻48例", 《中国社区医师（医学专业）》, vol. 12, no. 32, 4 January 2011 (2011-01-04), pages 109 - 110 * |
| 顾承美 等: "乌梅为主治疗癌肿晚期久泻久咳", 《新中医》, no. 3, 31 December 1994 (1994-12-31), pages 42 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655720A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition and preparation method thereof |
| CN103655933A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition as well as preparation method and application thereof |
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Application publication date: 20131023 |