CN103330746A - Traditional Chinese medicine composition, and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition, and preparation method and application thereof Download PDFInfo
- Publication number
- CN103330746A CN103330746A CN2013103041649A CN201310304164A CN103330746A CN 103330746 A CN103330746 A CN 103330746A CN 2013103041649 A CN2013103041649 A CN 2013103041649A CN 201310304164 A CN201310304164 A CN 201310304164A CN 103330746 A CN103330746 A CN 103330746A
- Authority
- CN
- China
- Prior art keywords
- lignum aquilariae
- aquilariae resinatum
- extract
- preparation
- cortex cinnamomi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a traditional Chinese medicine composition, and a preparation method and application thereof. The traditional Chinese medicine composition comprises an extract of cinnamon and aquilaria sinensis through water decoction and concentration, cinnamic aldehyde, aquilaria sinensis chloroform extract and aquilaria sinensis hexane extract at the ratio of (2-6): (1-6):(3-5):(2-8). The composition has an excellent curative effect for cancer chemotherapy related diarrhea and also has a synergistic effect for cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition of being made up of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing, the preparation method of this Chinese medicine composition and this Chinese medicine composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.The chemotherapy prescription with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but at present still unmatchful anticancer disease chemotherapy have the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Cortex Cinnamomi is dried bark and the thick branch skin of Lauraceae aiphyllium plant Cortex Cinnamomi Cinnamomum cassia Presl, cures mainly deficiency and coldness and vomits and diarrhoea.Lignum Aquilariae Resinatum is thymelaeceae plant Lignum Aquilariae Resinatum
Aquilaria sinensis(Lour.) the dry stem of Spreng, the property of medicine: bitter, sour, puckery, flat, return lung, large intestine channel; The relieving diarrhea with astringents effect is arranged, and is to control chronic diarrhea; Chronic dysentery; The medicine commonly used of proctoptosis, in recent years, Cortex Cinnamomi and Lignum Aquilariae Resinatum antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, be the compositions of novelty, and proved that it has fabulous curative effect and can finish the present invention thus simultaneously to chemotherapeutic sensitivity cancer chemotherapy dependency diarrhoea.
Summary of the invention
A kind of Chinese medicine composition provided by the invention is made of in the ratio of 2-6:1-6:3-5:2-8 by weight Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 6:6:5:8.
Wherein, Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration preparation method of extract is in the above-mentioned Chinese medicine composition:
Be that 1:1 gets Cortex Cinnamomi, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely.
The preparation method of cinnamic aldehyde is in the above-mentioned Chinese medicine composition:
Cortex Cinnamomi is pulverized, take by weighing the Cortex Cinnamomi powder with 95% ethanol in the apparatus,Soxhlet's heating extraction, filtration, behind the decompression recycling ethanol, carry out steam distillation, in distillate, add NaCl, ether divides 3 extractions, merge ether extraction liquid, add an amount of anhydrous calcium chloride desiccant after dry 30 minutes, remove desiccant, reclaim ether, use distilling under reduced pressure then instead, collect 150-151 ℃ fraction, namely get cinnamic aldehyde.
Lignum Aquilariae Resinatum chloroform extract preparation method is in the above-mentioned Chinese medicine composition:
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract.
Lignum Aquilariae Resinatum hexane extraction thing preparation method is in the above-mentioned Chinese medicine composition:
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
The present invention also provides the preferred manufacturing procedure of this Chinese medicine composition, comprising:
(1) preparation of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract
Be that 1:1 gets Cortex Cinnamomi, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely.
(2) preparation of cinnamic aldehyde
Cortex Cinnamomi is pulverized, take by weighing the Cortex Cinnamomi powder with 95% ethanol in the apparatus,Soxhlet's heating extraction, filtration, behind the decompression recycling ethanol, carry out steam distillation, in distillate, add NaCl, ether divides 3 extractions, merge ether extraction liquid, add an amount of anhydrous calcium chloride desiccant after dry 30 minutes, remove desiccant, reclaim ether, use distilling under reduced pressure then instead, collect 150-151 ℃ fraction, namely get cinnamic aldehyde.
(3) Lignum Aquilariae Resinatum chloroform extract preparation
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract.
(4) Lignum Aquilariae Resinatum hexane extraction thing preparation
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
(5) choose Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing in proportion, evenly mix, namely.
The preparation method of above-mentioned Chinese medicine composition also comprises: the product that step (5) makes is further made capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of Chinese medicine composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, Chinese medicine composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this Chinese medicine composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this Chinese medicine composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this Chinese medicine composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 2-6:1-6:3-5:2-8, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 6:6:5:8, this is that the inventor pays a large amount of creative works by keeping punching and obtains on the paper basis of delivering before, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this Chinese medicine composition Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and each component of Lignum Aquilariae Resinatum hexane extraction thing all can obtain by conventional means, some of them component for example cinnamic aldehyde also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of Chinese medicine composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract
Be that 1:1 gets Cortex Cinnamomi, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely.
(2) preparation of cinnamic aldehyde
Cortex Cinnamomi is pulverized, take by weighing the Cortex Cinnamomi powder with 95% ethanol in the apparatus,Soxhlet's heating extraction, filtration, behind the decompression recycling ethanol, carry out steam distillation, in distillate, add NaCl, ether divides 3 extractions, merge ether extraction liquid, add an amount of anhydrous calcium chloride desiccant after dry 30 minutes, remove desiccant, reclaim ether, use distilling under reduced pressure then instead, collect 150-151 ℃ fraction, namely get cinnamic aldehyde.
(3) Lignum Aquilariae Resinatum chloroform extract preparation
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, and decompression and solvent recovery namely gets the Lignum Aquilariae Resinatum chloroform extract.
(4) Lignum Aquilariae Resinatum hexane extraction thing preparation
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible, with hexane extraction 3 times, decompression and solvent recovery namely gets Lignum Aquilariae Resinatum hexane extraction thing.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take by weighing Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract 24mg, cinnamic aldehyde 24mg, Lignum Aquilariae Resinatum chloroform extract 20mg and the Lignum Aquilariae Resinatum hexane extraction thing 32mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of Chinese medicine composition tablet of the present invention
Take by weighing Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract 24mg, cinnamic aldehyde 24mg, Lignum Aquilariae Resinatum chloroform extract 20mg and the Lignum Aquilariae Resinatum hexane extraction thing 32mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 6:6:5:8.Inventor's compositions that the multiple proportioning combination between 2-6:1-6:3-5:2-8 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the Chinese medicine composition in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of traditional Chinese medicine composition for treating chemotherapy dependency diarrhoea effect
1, test material
(1) Cortex Cinnamomi, Lignum Aquilariae Resinatum is purchased in medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is cinnamic aldehyde 10g.kg
-1(being equivalent to contain the crude drug amount), D group Lignum Aquilariae Resinatum chloroform extract mixes 10g.kg with Lignum Aquilariae Resinatum hexane extraction thing in the 5:8 ratio
-1(being equivalent to contain the crude drug amount), the E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Chinese medicine composition group diarrhoea incidence rate of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of Chinese medicine composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group Chinese medicine composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5 ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Chinese medicine composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 Chinese medicine composition of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 32.5 |
| Compositions is low+cyclophosphamide | 38.4 |
| Height+cyclophosphamide | 48.9 |
Claims (5)
1. Chinese medicine composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition is made of in the ratio of 2-6:1-6:3-5:2-8 by weight Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing
Wherein Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration preparation method of extract is:
Be that 1:1 gets Cortex Cinnamomi, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely;
Wherein the preparation method of cinnamic aldehyde is:
Cortex Cinnamomi is pulverized, take by weighing the Cortex Cinnamomi powder with 95% ethanol in the apparatus,Soxhlet's heating extraction, filtration, behind the decompression recycling ethanol, carry out steam distillation, in distillate, add NaCl, ether divides 3 extractions, merge ether extraction liquid, add an amount of anhydrous calcium chloride desiccant after dry 30 minutes, remove desiccant, reclaim ether, use distilling under reduced pressure then instead, collect 150-151 ℃ fraction, namely get cinnamic aldehyde;
Wherein the preparation method of Lignum Aquilariae Resinatum chloroform extract is:
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, decompression and solvent recovery, namely;
Wherein the preparation method of Lignum Aquilariae Resinatum hexane extraction thing is:
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible is with hexane extraction 3 times, decompression and solvent recovery, namely.
2. Chinese medicine composition as claimed in claim 1 is characterized in that wherein the usage ratio of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 6:6:5:8.
3. as the preparation method of the Chinese medicine composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract
Be that 1:1 gets Cortex Cinnamomi, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely;
(2) preparation of cinnamic aldehyde
Cortex Cinnamomi is pulverized, take by weighing the Cortex Cinnamomi powder with 95% ethanol in the apparatus,Soxhlet's heating extraction, filtration, behind the decompression recycling ethanol, carry out steam distillation, in distillate, add NaCl, ether divides 3 extractions, merge ether extraction liquid, add an amount of anhydrous calcium chloride desiccant after dry 30 minutes, remove desiccant, reclaim ether, use distilling under reduced pressure then instead, collect 150-151 ℃ fraction, namely get cinnamic aldehyde;
(3) preparation of Lignum Aquilariae Resinatum chloroform extract
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible is used equal-volume chloroform extraction 3 times, decompression and solvent recovery, namely;
(4) preparation of Lignum Aquilariae Resinatum hexane extraction thing
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, the suitable quantity of water suspendible is with hexane extraction 3 times, decompression and solvent recovery, namely;
(5) choose Cortex Cinnamomi Lignum Aquilariae Resinatum water boiling concentration extract, cinnamic aldehyde, Lignum Aquilariae Resinatum chloroform extract in proportion and evenly mix with Lignum Aquilariae Resinatum hexane extraction thing, namely.
4. the preparation method of Chinese medicine composition as claimed in claim 3 is characterized in that also comprising: the product that step (5) makes is further made capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification by the pharmacy means of routine.
5. as the purposes of each described Chinese medicine composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103041649A CN103330746A (en) | 2013-07-19 | 2013-07-19 | Traditional Chinese medicine composition, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103041649A CN103330746A (en) | 2013-07-19 | 2013-07-19 | Traditional Chinese medicine composition, and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103330746A true CN103330746A (en) | 2013-10-02 |
Family
ID=49238996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103041649A Pending CN103330746A (en) | 2013-07-19 | 2013-07-19 | Traditional Chinese medicine composition, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103330746A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655933A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition as well as preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103182006A (en) * | 2013-03-27 | 2013-07-03 | 张宗升 | Traditional chinese medicine composition and preparation method thereof |
| CN103191194A (en) * | 2013-03-29 | 2013-07-10 | 阚兆云 | Traditional chinese medicine extract and preparation method thereof |
-
2013
- 2013-07-19 CN CN2013103041649A patent/CN103330746A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103182006A (en) * | 2013-03-27 | 2013-07-03 | 张宗升 | Traditional chinese medicine composition and preparation method thereof |
| CN103191194A (en) * | 2013-03-29 | 2013-07-10 | 阚兆云 | Traditional chinese medicine extract and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655933A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Traditional Chinese medicine composition as well as preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103191194A (en) | Traditional chinese medicine extract and preparation method thereof | |
| CN103191193A (en) | Traditional chinese medicine extract and preparation method thereof | |
| CN103340926A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103356831A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103385922A (en) | Natural pharmaceutical composition and preparation method | |
| CN103340929A (en) | Natural drug extract | |
| CN103356769A (en) | Natural drug extract, preparation method and application thereof | |
| CN103341109A (en) | Natural drug extract and preparation method thereof | |
| CN103340970A (en) | Traditional chinese medicine composition | |
| CN103223085A (en) | Chinese medicinal extract | |
| CN103316115A (en) | Traditional Chinese medicinal composition and preparation method thereof | |
| CN103251771A (en) | Traditional chinese medicine composition | |
| CN103223004A (en) | Chinese medicinal composition | |
| CN103330746A (en) | Traditional Chinese medicine composition, and preparation method and application thereof | |
| CN103340968A (en) | Natural medicine composition and preparation method thereof | |
| CN103330745A (en) | Natural pharmaceutical composition | |
| CN103316189A (en) | Natural pharmaceutical composition | |
| CN103330753A (en) | Traditional Chinese medicine composition and preparation method thereof | |
| CN103393758A (en) | Natural pharmaceutical composition | |
| CN103301232A (en) | Natural pharmaceutical composition as well as preparation method and application thereof | |
| CN103316191A (en) | Natural medicine extract | |
| CN103393847A (en) | Traditional Chinese medicine composition, preparation method and application thereof | |
| CN103316153A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103330730A (en) | Natural medicine composition and preparation method thereof | |
| CN103356814A (en) | Chinese medicinal composition, its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131002 |