CN103393847A - Traditional Chinese medicine composition, preparation method and application thereof - Google Patents
Traditional Chinese medicine composition, preparation method and application thereof Download PDFInfo
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- CN103393847A CN103393847A CN2013103052412A CN201310305241A CN103393847A CN 103393847 A CN103393847 A CN 103393847A CN 2013103052412 A CN2013103052412 A CN 2013103052412A CN 201310305241 A CN201310305241 A CN 201310305241A CN 103393847 A CN103393847 A CN 103393847A
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Abstract
The invention relates to a traditional Chinese medicine composition, a preparation method and an application thereof. The traditional Chinese medicine composition is composed of garden burnet and aquilaria sinensis water-decocted concentrated extractive, garden burnet total saponins, chloroform extractive of aquilaria sinensis and hexane extractive of aquilaria sinensis at the ratio of (2-3):(4-6):(2-8):(4-8). The composition in the invention has an excellent curative effect on cancer chemotherapy-induced diarrhea and has a synergism effect on cancer chemotherapy at the same time.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition that is comprised of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing, the preparation method of this Chinese medicine composition and this Chinese medicine composition are in the purposes for the preparation of in reducing the cancer chemotherapy induced diarrhea and treatment of cancer being played the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is alone or unite with the toxicity such as all can cause that the patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the antagonism cancer chemotherapy, has the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating the cancer chemotherapy induced diarrhea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Radix Sanguisorbae is the dry root of rosaceous plant Radix Sanguisorbae or the Radix Sanguisorbae that comes into leaves, the dysentery of stopping blooding, and " Japan hanako materia medica " record Radix Sanguisorbae is used for blood disorders, dysentery with red and white feces and watery diarrhea.Lignum Aquilariae Resinatum is the Isolated From Thymelaeaceae Species Lignum Aquilariae Resinatum
Aquilaria sinensis(Lour.) the dry stem of Spreng, the property of medicine: bitter, sour, puckery, flat, return lung, large intestine channel; The relieving diarrhea with astringents effect is arranged, and is to control chronic diarrhea; Chronic dysentery; The common medicine of proctoptosis, in recent years, Radix Sanguisorbae and Lignum Aquilariae Resinatum antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained finally optimal drug ratio compositions of the present invention, and proved that it has fabulous curative effect and can, simultaneously to chemotherapeutic sensitivity, complete thus the present invention the cancer chemotherapy induced diarrhea.
Summary of the invention
A kind of Chinese medicine composition provided by the invention, consist of in the ratio of 2-3:4-6:2-8:4-8 by weight Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 3:6:8:8.
Wherein, Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned Chinese medicine composition, the preparation method of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract is:
Be that 1-4:1 gets Radix Sanguisorbae, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtain.
In above-mentioned Chinese medicine composition, the preparation method of radix sanguisorbae total saponin is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
In above-mentioned Chinese medicine composition, Lignum Aquilariae Resinatum chloroform extract preparation method is:
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible, use equal-volume chloroform extraction 3 times, and decompression and solvent recovery, obtain the Lignum Aquilariae Resinatum chloroform extract.
In above-mentioned Chinese medicine composition, Lignum Aquilariae Resinatum hexane extraction thing preparation method is:
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter to obtain methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, and the suitable quantity of water suspendible is used hexane extraction 3 times, and decompression and solvent recovery, obtain Lignum Aquilariae Resinatum hexane extraction thing.
The present invention also provides the preferred preparation method of this Chinese medicine composition, comprising:
(1) preparation of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract
Be that 1-4:1 gets Radix Sanguisorbae, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtain.
(2) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
(3) Lignum Aquilariae Resinatum chloroform extract preparation
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible, use equal-volume chloroform extraction 3 times, and decompression and solvent recovery, obtain the Lignum Aquilariae Resinatum chloroform extract.
(4) Lignum Aquilariae Resinatum hexane extraction thing preparation
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter to obtain methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, and the suitable quantity of water suspendible is used hexane extraction 3 times, and decompression and solvent recovery, obtain Lignum Aquilariae Resinatum hexane extraction thing.
(5) choose in proportion Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing, evenly mix, obtain.
The preparation method of above-mentioned Chinese medicine composition also comprises: the product that step (5) makes is further made by the pharmacy means of routine capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
The beneficial effect of Chinese medicine composition of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, Chinese medicine composition of the present invention causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has obvious antagonism.
2, proved that this Chinese medicine composition can strengthen the inhibitory action of chemotherapeutics to tumor, adopted mouse tumor model, observed this Chinese medicine composition and the cyclophosphamide combined inhibitory action to mouse interior tumor.Research shows: this Chinese medicine composition can obviously increase the inhibitory action of cyclophosphamide for tumor.
3, the inventor makes up Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing originally, and by great many of experiments, determine consumption proportion between these components, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 2-3:4-6:2-8:4-8, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 3:6:8:8, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this Chinese medicine composition Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and each component of Lignum Aquilariae Resinatum hexane extraction thing all can obtain by conventional means, wherein component such as radix sanguisorbae total saponin also can be buied by business, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of Chinese medicine composition of the present invention below by embodiment and test example, with and the beneficial effect applied in diarrhea and antitumor.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract
Be that 1-4:1 gets Radix Sanguisorbae, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtain.
(2) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
(3) Lignum Aquilariae Resinatum chloroform extract preparation
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible, use equal-volume chloroform extraction 3 times, and decompression and solvent recovery, obtain the Lignum Aquilariae Resinatum chloroform extract.
(4) Lignum Aquilariae Resinatum hexane extraction thing preparation
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter to obtain methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, and the suitable quantity of water suspendible is used hexane extraction 3 times, and decompression and solvent recovery, obtain Lignum Aquilariae Resinatum hexane extraction thing.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take respectively Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract 12mg, radix sanguisorbae total saponin 24mg, Lignum Aquilariae Resinatum chloroform extract 32mg and the Lignum Aquilariae Resinatum hexane extraction thing 32mg of above-mentioned steps preparation, take simultaneously starch 395mg, magnesium stearate 5mg, evenly mix, be filled to capsule.
(2) preparation of Chinese medicine composition tablet of the present invention
Take respectively Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract 12mg, radix sanguisorbae total saponin 24mg, Lignum Aquilariae Resinatum chloroform extract 32mg and the Lignum Aquilariae Resinatum hexane extraction thing 32mg of above-mentioned steps preparation, take simultaneously starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate after granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 3:6:8:8.Inventor's compositions that the multiple proportioning combination between 2-3:4-6:2-8:4-8 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the Chinese medicine composition in this amount ranges can obtain good synergism, to the cancer chemotherapy induced diarrhea, can play good effect and the treatment of cancer is had potentiation.Below the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of traditional Chinese medicine composition for treating Chemotherapy-induced Diarrhea effect
1, test material
(1) Radix Sanguisorbae, Lignum Aquilariae Resinatum, be purchased from medical material market.
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is radix sanguisorbae total saponin 10g.kg
-1(being equivalent to contain the crude drug amount), D group Lignum Aquilariae Resinatum chloroform extract mixes 10g.kg with Lignum Aquilariae Resinatum hexane extraction thing in the 8:8 ratio
-1(being equivalent to contain the crude drug amount), the E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copy the Chemotherapy-induced Diarrhea model.B, C, D, E group, with corresponding extract gavage 8 days, is placed in metabolic cage with mice continuously, and at the bottom of cage, the white filter paper of paving, to observe the stool situation, and is observed mice crissum situation and Mus tail fecal pollution situation, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wet and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Chinese medicine composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of Chinese medicine composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
The employing 5-7 days that goes down to posterity, well-grown milky ascites, be diluted to tumor cell suspension with appropriate physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).The inoculation next day random packet, experimental group Chinese medicine composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus gavage 0.5 ml, give cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and by following formula, calculates tumor control rate (%):
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Chinese medicine composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 Chinese medicine composition of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 32.5 |
| Compositions is low+cyclophosphamide | 42.7 |
| Height+cyclophosphamide | 56.1 |
Claims (5)
1. one kind is reduced the cancer chemotherapy induced diarrhea and the treatment cancer is played the Chinese medicine composition of potentiation, it is characterized in that said composition consists of in the ratio of 2-3:4-6:2-8:4-8 by weight Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing
Wherein the preparation method of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract is:
Be that 1-4:1 gets Radix Sanguisorbae, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtain;
Wherein the preparation method of radix sanguisorbae total saponin is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product;
Wherein the preparation method of Lignum Aquilariae Resinatum chloroform extract is:
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible, use equal-volume chloroform extraction 3 times, and decompression and solvent recovery, obtain the Lignum Aquilariae Resinatum chloroform extract;
Wherein the preparation method of Lignum Aquilariae Resinatum hexane extraction thing is:
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter to obtain methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, and the suitable quantity of water suspendible is used hexane extraction 3 times, and decompression and solvent recovery, obtain Lignum Aquilariae Resinatum hexane extraction thing.
2. Chinese medicine composition as claimed in claim 1, is characterized in that wherein the usage ratio of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and Lignum Aquilariae Resinatum hexane extraction thing is 3:6:8:8.
3., as the preparation method of the Chinese medicine composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract
Be that 1-4:1 gets Radix Sanguisorbae, Lignum Aquilariae Resinatum according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtain;
(2) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product;
(3) preparation of Lignum Aquilariae Resinatum chloroform extract
Get the Lignum Aquilariae Resinatum coarse powder, 95% alcohol reflux 3 times, merge extractive liquid, and concentrating under reduced pressure, with the gained crude extract, the suitable quantity of water suspendible, use equal-volume chloroform extraction 3 times, and decompression and solvent recovery, obtain the Lignum Aquilariae Resinatum chloroform extract;
(4) preparation of Lignum Aquilariae Resinatum hexane extraction thing
The Lignum Aquilariae Resinatum coarse powder methanol room temperature of drying was extracted for 2 weeks, filter to obtain methanol extract liquid, reclaim under reduced pressure obtains methanolic extract, and the suitable quantity of water suspendible is used hexane extraction 3 times, and decompression and solvent recovery, obtain Lignum Aquilariae Resinatum hexane extraction thing;
(5) choose in proportion Radix Sanguisorbae Lignum Aquilariae Resinatum water boiling concentration extract, radix sanguisorbae total saponin, Lignum Aquilariae Resinatum chloroform extract and evenly mix with Lignum Aquilariae Resinatum hexane extraction thing, obtain.
4. the preparation method of Chinese medicine composition as claimed in claim 3, characterized by further comprising: the product that step (5) makes is further made by the pharmacy means of routine capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
5. as the purposes of the described Chinese medicine composition of claim 1-2 any one in preparing the medicine that reduces the reaction of cancer chemotherapy induced diarrhea and the treatment cancer is played potentiation.
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Application publication date: 20131120 |