CN103356768A - Natural pharmaceutical composition and preparation method thereof - Google Patents
Natural pharmaceutical composition and preparation method thereof Download PDFInfo
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- CN103356768A CN103356768A CN2013102825201A CN201310282520A CN103356768A CN 103356768 A CN103356768 A CN 103356768A CN 2013102825201 A CN2013102825201 A CN 2013102825201A CN 201310282520 A CN201310282520 A CN 201310282520A CN 103356768 A CN103356768 A CN 103356768A
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Abstract
The invention relates to a natural pharmaceutical composition and a preparation method thereof. The natural pharmaceutical composition is prepared from a water-decocted and concentrated extract of myrobalan and smoked plum, a 50% ethanol extract of myrobalan and an ethanol extract of smoked plum according to the ratio of (2-7): (7-8): (3-5). The composition has a good curative effect on related diarrhea of cancer chemotherapy, and simultaneously can play a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition that is comprised of Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing the cancer chemotherapy induced diarrhea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic, no matter alone or unite with the toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea is again Chemotherapy-induced Diarrhea behind the chemotherapy of tumors.In recent years emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but there is no at present the Chinese patent medicine that the antagonism cancer chemotherapy has alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating the cancer chemotherapy induced diarrhea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
The Fructus Chebulae calls tender Fructus Chebulae, chebule, scolds multitude, scolds pears, son with the wind.Sour in the mouth is puckery; Property is put down, and returns lung, large intestine channel; The relieving diarrhea with astringents effect is arranged, and is to control chronic diarrhea; Chronic dysentery; The common medicine of proctoptosis.Fructus Mume is that the dry almost ripe fruit by rose department plant plum processes, and can astringe the lung astringing intestine to stop diarrhea.Be used for chronic diarrhea; Dysentery; The diseases such as vomiting.In recent years, Fructus Chebulae and Fructus Mume antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained at last optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can play simultaneously the effect that suppresses tumor the cancer chemotherapy induced diarrhea, has finished the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention is made of in the ratio of 2-7:7-8:3-5 by weight Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction.
Above-mentioned natural drug composition, wherein the usage ratio of Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction is 7:8:5.
Wherein, Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, the preparation method of Fructus Chebulae's Fructus Mume water boiling concentration extract is in the above-mentioned natural drug composition:
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Fructus Mume, distilled water immersion, and heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product.
The preparation method of Fructus Chebulae's 50% ethanol extraction is in the above-mentioned natural drug composition:
The Fructus Chebulae of drying is pulverized, cross the 20-40 mesh sieve, with 50% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery gets dry extract, extractum is put to room temperature after being diluted to suspension with hot water, and ethyl acetate extraction reclaims solvent, drying is pulverized, and sieves, and get final product.
Fructus Mume ethanol extraction preparation method is in the above-mentioned natural drug composition:
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Fructus Chebulae's Fructus Mume water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Fructus Mume, distilled water immersion, and heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product.
(2) preparation of Fructus Chebulae's 50% ethanol extraction
The Fructus Chebulae of drying is pulverized, cross the 20-40 mesh sieve, with 50% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery gets dry extract, extractum is put to room temperature after being diluted to suspension with hot water, and ethyl acetate extraction reclaims solvent, drying is pulverized, and sieves, and get final product.
(3) Fructus Mume ethanol extraction preparation
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
(4) choose in proportion Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction, evenly mix, and get final product.
The preparation method of above-mentioned natural drug composition also comprises: the product that step (4) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of natural drug composition of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes studies show that of diarrhea of mouse model at irinotecan: the diarrhoea reaction for the irinotecan induced mice has obvious antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction originally, and determine consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our repeatedly experimentation, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-7:7-8:3-5, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 7:8:5, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Fructus Chebulae Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and each component of Fructus Mume ethanol extraction all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Chebulae's Fructus Mume water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Fructus Mume, distilled water immersion, and heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product.
(2) preparation of Fructus Chebulae's 50% ethanol extraction
The Fructus Chebulae of drying is pulverized, cross the 20-40 mesh sieve, with 50% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery gets dry extract, extractum is put to room temperature after being diluted to suspension with hot water, and ethyl acetate extraction reclaims solvent, drying is pulverized, and sieves, and get final product.
(3) Fructus Mume ethanol extraction preparation
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing respectively Fructus Chebulae's Fructus Mume water boiling concentration extract 35mg, Fructus Chebulae 50% ethanol extraction 40mg and the Fructus Mume ethanol extraction 25mg of above-mentioned steps preparation, take by weighing simultaneously starch 395mg, magnesium stearate 5mg, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing respectively Fructus Chebulae's Fructus Mume water boiling concentration extract 35mg, Fructus Chebulae 50% ethanol extraction 40mg and the Fructus Mume ethanol extraction 25mg of above-mentioned steps preparation, take by weighing simultaneously starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into every tablet of tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction is 7:8:5.Inventor's compositions that the multiple proportioning combination between 2-7:7-8:3-5 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and the treatment of cancer is had potentiation the cancer chemotherapy induced diarrhea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Fructus Chebulae, Fructus Mume
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Fructus Chebulae's Fructus Mume water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Fructus Chebulae 50% ethanol extraction 10g.kg
-1(being equivalent to contain the crude drug amount), D group Fructus Mume ethanol extraction 10g.kg
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies the Chemotherapy-induced Diarrhea model at every day 1 time.B, C, D, E group with corresponding extract gavage 8 days, places metabolic cage with mice continuously, spreads white filter paper at the bottom of the cage observing the stool situation, and observes mice crissum situation and Mus tail fecal pollution situation, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wet and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).The inoculation next day random packet, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus gavage 0.5 ml gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(such as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 32.1 |
| Compositions is low+cyclophosphamide | 42.8 |
| Height+cyclophosphamide | 51.7 |
Claims (5)
1. one kind is reduced the cancer chemotherapy induced diarrhea and the treatment cancer is played the natural drug composition of potentiation, it is characterized in that said composition is made of in the ratio of 2-7:7-8:3-5 by weight Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction
Wherein the preparation method of Fructus Chebulae's Fructus Mume water boiling concentration extract is:
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Fructus Mume, distilled water immersion, and heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product;
Wherein the preparation method of Fructus Chebulae's 50% ethanol extraction is:
The Fructus Chebulae of drying is pulverized, cross the 20-40 mesh sieve, with 50% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery gets dry extract, extractum is put to room temperature after being diluted to suspension with hot water, and ethyl acetate extraction reclaims solvent, drying is pulverized, and sieves, and get final product;
Wherein the preparation method of Fructus Mume ethanol extraction is:
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction and Fructus Mume ethanol extraction is 7:8:5.
3. such as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Fructus Chebulae's Fructus Mume water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Fructus Chebulae, Fructus Mume, distilled water immersion, and heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, and get final product;
(2) preparation of Fructus Chebulae's 50% ethanol extraction
The Fructus Chebulae of drying is pulverized, cross the 20-40 mesh sieve, with 50% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery gets dry extract, extractum is put to room temperature after being diluted to suspension with hot water, and ethyl acetate extraction reclaims solvent, drying is pulverized, and sieves, and get final product;
(3) preparation of Fructus Mume ethanol extraction
The Fructus Mume of drying is pulverized, sieved, 75% alcohol reflux filters, and medicinal residues add 75% alcohol reflux, filter, 2 filtrates are merged, centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method is concentrated, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, and get final product;
(4) choose in proportion Fructus Chebulae's Fructus Mume water boiling concentration extract, Fructus Chebulae's 50% ethanol extraction evenly mixes with the Fructus Mume ethanol extraction, and get final product.
4. the preparation method of natural drug composition as claimed in claim 3 also comprises: the product that step (4) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
5. such as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy induced diarrhea and the treatment cancer is played potentiation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048269A (en) * | 2017-12-30 | 2018-05-18 | 佛山科学技术学院 | A kind of myrobalan's beer and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091315A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Ginger and dark plum fruit composition and preparation method thereof, and application of ginger and dark plum fruit composition in preparation of attenuation and synergy medicaments for radiotherapy and chemotherapy of cancers |
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2013
- 2013-07-08 CN CN2013102825201A patent/CN103356768A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091315A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Ginger and dark plum fruit composition and preparation method thereof, and application of ginger and dark plum fruit composition in preparation of attenuation and synergy medicaments for radiotherapy and chemotherapy of cancers |
Non-Patent Citations (2)
| Title |
|---|
| VAIBHAV AHER ET AL.: "Immunomodulatory Activity of Alcohol Extract of Terminalia chebula Retz Combretaceae", 《TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH》 * |
| 王金华 等: "诃子的药理作用研究进展", 《中国民族医药杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048269A (en) * | 2017-12-30 | 2018-05-18 | 佛山科学技术学院 | A kind of myrobalan's beer and preparation method thereof |
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Application publication date: 20131023 |