CN103251771A - Traditional chinese medicine composition - Google Patents
Traditional chinese medicine composition Download PDFInfo
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- CN103251771A CN103251771A CN2013101325222A CN201310132522A CN103251771A CN 103251771 A CN103251771 A CN 103251771A CN 2013101325222 A CN2013101325222 A CN 2013101325222A CN 201310132522 A CN201310132522 A CN 201310132522A CN 103251771 A CN103251771 A CN 103251771A
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Abstract
The invention relates to a traditional Chinese medicine composition. The composition comprises the extract obtained by decocting garden burnet root and Chinese yam with water and concentrating the product, total saponins from garden burnet root, ethanol extract of Chinese yam and Chinese yam polysaccharides in a ratio of (3-6):(2-3):(3-8):(4-8). The composition has an excellent curative effect on cancer chemotherapy-induced diarrhea and simultaneously has a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.The chemotherapy prescription with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but at present still unmatchful anticancer disease chemotherapy have the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Radix Sanguisorbae is the dry root of rosaceous plant Radix Sanguisorbae or the Radix Sanguisorbae that comes into leaves, and the dysentery of stopping blooding is used for blood disorders, dysentery with red and white feces and watery diarrhea.Multiple efficacies such as Rhizoma Dioscoreae has spleen invigorating, tonifying the lung, reinforces the kidney, beneficial essence have therapeutical effect to diarrhea due to hypofunction of the spleen.In recent years, Radix Sanguisorbae and Rhizoma Dioscoreae antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, and proved that it has fabulous curative effect and can finish the present invention thus simultaneously to chemotherapeutic sensitivity cancer chemotherapy dependency diarrhoea.
Summary of the invention
A kind of natural drug composition provided by the invention, by Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide by weight in 3-6: the ratio of 2-3: 3-8: 4-8 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 6: 3: 8: 8.
Wherein, Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction can obtain by well known to a person skilled in the art that conventional method is purified, and the natural drug of the present invention that these compositions that conventional method obtains or the commercial one-tenth of buying are grouped into all can obtain identical curative effect.
Preferably, Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
Be 1-5 according to proportion compatibility: 1 gets Radix Sanguisorbae, Rhizoma Dioscoreae, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, namely.
The preparation method of radix sanguisorbae total saponin is in the above-mentioned natural drug composition:
Radix Sanguisorbae is pulverized, and 70% soak with ethanol is spent the night, reflux 3 times, and merge extractive liquid, reclaims ethanol, and the gained solid is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are namely.
Rhizoma Dioscoreae ethanol extraction preparation method is in the above-mentioned natural drug composition:
Get dried Rhizoma Dioscoreae powder, with 95% alcohol reflux 3 times, each 4h, remove filtrate, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h, collect extracting solution, centrifugal, supernatant concentration, add 4 times of volume of ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone, ether washing precipitation respectively, lyophilization, namely.
The Chinese yam polysaccharide preparation method is in the above-mentioned natural drug composition:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, with tap water and distill water dialysis, add 4 times of volume ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone and ether washing precipitation respectively, lyophilization, namely.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract
Be 1-5 according to proportion compatibility: 1 gets Radix Sanguisorbae, Rhizoma Dioscoreae, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, namely.
(2) preparation of radix sanguisorbae total saponin
Radix Sanguisorbae is pulverized, and 70% soak with ethanol is spent the night, reflux 3 times, and merge extractive liquid, reclaims ethanol, and the gained solid is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are namely.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get dried Rhizoma Dioscoreae powder, with 95% alcohol reflux 3 times, each 4h, remove filtrate, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h, collect extracting solution, centrifugal, supernatant concentration, add 4 times of volume of ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone, ether washing precipitation respectively, lyophilization, namely.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, with tap water and distill water dialysis, add 4 times of volume ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone and ether washing precipitation respectively, lyophilization, namely.
(5) choose Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide in proportion, evenly mix, namely.
The preparation method of above-mentioned natural drug composition also comprises: the product that step (5) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition performance synergism, obtain unexpected effect.Through experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 3-6: 2-3: 3-8: produced unexpected synergism during 4-8, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 6: 3: 8: 8 o'clock especially obvious, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this proportioning and optimum amount ratio by disclosed content.
4, this natural drug composition Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and each component of Chinese yam polysaccharide all can obtain by conventional means, Chinese yam polysaccharide also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract
Be 1-5 according to proportion compatibility: 1 gets Radix Sanguisorbae, Rhizoma Dioscoreae, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, namely.
(2) preparation of radix sanguisorbae total saponin
Radix Sanguisorbae is pulverized, and 70% soak with ethanol is spent the night, reflux 3 times, and merge extractive liquid, reclaims ethanol, and the gained solid is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are namely.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get dried Rhizoma Dioscoreae powder, with 95% alcohol reflux 3 times, each 4h, remove filtrate, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h, collect extracting solution, centrifugal, supernatant concentration, add 4 times of volume of ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone, ether washing precipitation respectively, lyophilization, namely.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, with tap water and distill water dialysis, add 4 times of volume ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone and ether washing precipitation respectively, lyophilization, namely.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract 24mg, radix sanguisorbae total saponin 12mg, Rhizoma Dioscoreae ethanol extraction 32mg and the Chinese yam polysaccharide 32mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract 24mg, radix sanguisorbae total saponin 12mg, Rhizoma Dioscoreae ethanol extraction 32mg and the Chinese yam polysaccharide 32mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 6: 3: 8: 8.The inventor to above-mentioned each composition usage ratio at 3-6: 2-3: 3-8: the compositions that the multiple proportioning combination in the time of between the 4-8 obtains has been carried out and last identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and to cancer chemotherapy potentiation to cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Radix Sanguisorbae, Rhizoma Dioscoreae is purchased in medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is radix sanguisorbae total saponin 10g.kg
-1(being equivalent to contain the crude drug amount), D group Rhizoma Dioscoreae ethanol extraction mixes 10g.kg with Chinese yam polysaccharide in 1: 1 ratio
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, the degree of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract group chemotherapy dependency diarrhoea is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
(1) to mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 6 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide by weight in 3-6: the ratio of 2-3: 3-8: 4-8 is formed
Wherein Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration preparation method of extract is:
Be 1-5 according to proportion compatibility: 1 gets Radix Sanguisorbae, Rhizoma Dioscoreae, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, namely;
Wherein the preparation method of radix sanguisorbae total saponin is:
Radix Sanguisorbae is pulverized, and 70% soak with ethanol is spent the night, reflux 3 times, and merge extractive liquid, reclaims ethanol, and the gained solid is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are namely;
Wherein the preparation method of Rhizoma Dioscoreae ethanol extraction is:
Get dried Rhizoma Dioscoreae powder, with 95% alcohol reflux 3 times, each 4h, remove filtrate, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h, collect extracting solution, centrifugal, supernatant concentration, add 4 times of volume of ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone, ether washing precipitation respectively, lyophilization, namely;
Wherein the preparation method of Chinese yam polysaccharide is:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, with tap water and distill water dialysis, add 4 times of volume ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone and ether washing precipitation respectively, lyophilization, namely.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 6: 3: 8: 8.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract
Be 1-5 according to proportion compatibility: 1 gets Radix Sanguisorbae, Rhizoma Dioscoreae, distilled water immersion 1h, and heating decocts, and silk filters, medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure, namely;
(2) preparation of radix sanguisorbae total saponin
Radix Sanguisorbae is pulverized, and 70% soak with ethanol is spent the night, reflux 3 times, and merge extractive liquid, reclaims ethanol, and the gained solid is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying are namely;
(3) preparation of Rhizoma Dioscoreae ethanol extraction
Get dried Rhizoma Dioscoreae powder, with 95% alcohol reflux 3 times, each 4h, remove filtrate, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h, collect extracting solution, centrifugal, supernatant concentration, add 4 times of volume of ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone, ether washing precipitation respectively, lyophilization, namely;
(4) preparation of Chinese yam polysaccharide
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add 1/5 volume Sevag reagent, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, with tap water and distill water dialysis, add 4 times of volume ethanol, 4 ℃ are spent the night, centrifugal, collecting precipitation, use ethanol, acetone and ether washing precipitation respectively, lyophilization, namely;
(5) get Radix Sanguisorbae Rhizoma Dioscoreae water boiling concentration extract, radix sanguisorbae total saponin, Rhizoma Dioscoreae ethanol extraction in proportion and evenly mix with Chinese yam polysaccharide, namely.
4. the preparation method of natural drug composition as claimed in claim 3 is characterized in that also comprising: the product that step (5) makes is further made capsule or the tablet that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104510810A (en) * | 2013-10-08 | 2015-04-15 | 成都百草和济科技有限公司 | New application of radix sanguisorbae or extract thereof |
| CN105079175A (en) * | 2014-05-08 | 2015-11-25 | 成都百草和济科技有限公司 | Application of garden burnet or extract thereof in preparing veterinary drug for treating animal diarrhea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102258745A (en) * | 2011-06-16 | 2011-11-30 | 吴新治 | Antiviral antidiarrheal agent for treating autumn viral diarrhea and spleen-deficiency diarrhea in infants |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104510810A (en) * | 2013-10-08 | 2015-04-15 | 成都百草和济科技有限公司 | New application of radix sanguisorbae or extract thereof |
| CN104510810B (en) * | 2013-10-08 | 2017-11-03 | 成都百草和济科技有限公司 | The new application of garden burnet or its extract |
| CN105079175A (en) * | 2014-05-08 | 2015-11-25 | 成都百草和济科技有限公司 | Application of garden burnet or extract thereof in preparing veterinary drug for treating animal diarrhea |
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Application publication date: 20130821 |