CN103182016A - Traditional chinese medicine composition and preparation method thereof - Google Patents
Traditional chinese medicine composition and preparation method thereof Download PDFInfo
- Publication number
- CN103182016A CN103182016A CN 201310118664 CN201310118664A CN103182016A CN 103182016 A CN103182016 A CN 103182016A CN 201310118664 CN201310118664 CN 201310118664 CN 201310118664 A CN201310118664 A CN 201310118664A CN 103182016 A CN103182016 A CN 103182016A
- Authority
- CN
- China
- Prior art keywords
- fructus piperis
- ethanol
- piperis longi
- soak
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a natural medicine composition, a preparation method thereof, and an application of the composition in preparation of a toxicity reducing and efficacy enhancing medicine for cancer chemotherapy. The natural medicine composition is composed of a Chinese yam and fructus piperis longi extract after water decoction and concentration, Chinese yam polysaccharide, a fructus piperis longi ethanol extract and piperine in a ratio of (2-6): (3-6): (3-4): (5-9). The composition has an excellent curative effect on diarrhea related with the cancer chemotherapy, and can enhance the effect on the cancer chemotherapy simultaneously.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic no matter singly with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but at present still unmatchful anticancer disease chemotherapy have the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Rhizoma Dioscoreae is perennial voluble herb, Chinese medicine, vegetable dual-purpose, and motherland's medical science thinks, multiple efficacies such as Rhizoma Dioscoreae has spleen invigorating, tonifying the lung, reinforces the kidney, beneficial essence has therapeutical effect to diarrhea due to hypofunction of the spleen.Fructus Piperis Longi is the green fruit of Piperaceae plant Piper longumI., disease such as cure mainly coldness and pain in the epigastrium, vomit, have loose bowels.In recent years, Rhizoma Dioscoreae and Fructus Piperis Longi antitumaous effect are confirmed, especially Chinese yam polysaccharide and piperine, the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, be the compositions of novelty, and proved that it has fabulous curative effect and can play potentiation to cancer chemotherapy simultaneously cancer chemotherapy dependency diarrhoea, has finished the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, by Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine by weight in 2-3: the ratio of 6-9: 5-8: 1-5 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine is 3: 9: 8: 5.
Wherein, Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
Be 1: 1 or 1-6 according to proportion compatibility: 1 other ratios are got Rhizoma Dioscoreae, Fructus Piperis Longi, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract.
The preparation method of Chinese yam polysaccharide is in the above-mentioned natural drug composition:
Get dried Rhizoma Dioscoreae powder, with 95% ethanol 70 ℃ of reflux, extract, 3 times, each 4h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add 4 times of volume of ethanol, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, ethanol extraction, extract add the water redissolution and are made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, use tap water and distill water dialysis respectively, add 4 times of volume ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used ethanol respectively, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
Fructus Piperis Longi ethanol extraction preparation method is in the above-mentioned natural drug composition:
Get Fructus Piperis Longi, drying and crushing is sieved, and with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, namely.
Process for preparing piperine is in the above-mentioned natural drug composition:
The Fructus Piperis Longi granule is ground into 20 orders, with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged, and is evaporated to thick, concentrated solution is put cold place and was left standstill 1-2 days, separates out piperine, filter crude product, the crude product of drying is dissolved in the ether, and 10%KOH solution washing three times is collected ether layer, boil off ether and get the faint yellow solid powder, the gained powder is dissolved in the ethanol, and the activated carbon decolorizing recrystallization namely gets the piperine of the prismatic crystallization of monocline.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract
Be 1: 1 or 1-6 according to proportion compatibility: 1 other ratios are got Rhizoma Dioscoreae, Fructus Piperis Longi, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract.
(2) preparation of Chinese yam polysaccharide
Get dried Rhizoma Dioscoreae powder, with 95% ethanol 70 ℃ of reflux, extract, 3 times, each 4h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add 4 times of volume of ethanol, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, ethanol extraction, extract add the water redissolution and are made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, use tap water and distill water dialysis respectively, add 4 times of volume ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used ethanol respectively, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
(3) Fructus Piperis Longi ethanol extraction preparation
Get Fructus Piperis Longi, drying and crushing is sieved, and with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, namely.
(4) piperine preparation
The Fructus Piperis Longi granule is ground into 20 orders, with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged, and is evaporated to thick, concentrated solution is put cold place and was left standstill 1-2 days, separates out piperine, filter crude product, the crude product of drying is dissolved in the ether, and 10%KOH solution washing three times is collected ether layer, boil off ether and get the faint yellow solid powder, the gained powder is dissolved in the ethanol, and the activated carbon decolorizing recrystallization namely gets the piperine of the prismatic crystallization of monocline.
(5) choose Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine in proportion, evenly mix, namely.
The preparation method of above-mentioned natural drug composition, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-3: 6-9: 5-8: produced unexpected synergism during 1-5, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 3: 9: 8: 5 o'clock especially obvious, this is that the inventor pays a large amount of creative works by keeping punching and obtains on the paper basis of delivering before, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and each component of piperine all can obtain by conventional means, some of them component for example Chinese yam polysaccharide and piperine also can be buied by commerce, and such compositions itself has and obtains convenient, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract
Be 1: 1 or 1-6 according to proportion compatibility: 1 other ratios are got Rhizoma Dioscoreae, Fructus Piperis Longi, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
(2) preparation of Chinese yam polysaccharide
Get dried Rhizoma Dioscoreae powder, with 95% ethanol 70 ℃ of reflux, extract, 3 times, each 4h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add 4 times of volume of ethanol, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, ethanol extraction, extract add the water redissolution and are made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, use tap water and distill water dialysis respectively, add 4 times of volume ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used ethanol respectively, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide.
(3) Fructus Piperis Longi ethanol extraction preparation
Get Fructus Piperis Longi, drying and crushing is sieved, and with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, water bath method, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, namely.
(4) piperine preparation
The Fructus Piperis Longi granule is ground into 20 orders, with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged, and is evaporated to thick, concentrated solution is put cold place and was left standstill 1-2 days, separates out piperine, filter crude product, the crude product of drying is dissolved in the ether, and 10%KOH solution washing three times is collected ether layer, boil off ether and get the faint yellow solid powder, the gained powder is dissolved in the ethanol, and the activated carbon decolorizing recrystallization namely gets the piperine of the prismatic crystallization of monocline.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract 12mg, Chinese yam polysaccharide 36mg, Fructus Piperis Longi ethanol extraction 32mg and the piperine 20mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract 12mg, Chinese yam polysaccharide 36mg, Fructus Piperis Longi ethanol extraction 32mg and the piperine 20mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine is 3: 9: 8: 5.The inventor to above-mentioned each composition usage ratio at 2-3: 6-9: 5-8: the compositions that the multiple proportioning combination in the time of between the 1-5 obtains has been carried out and last identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Rhizoma Dioscoreae, Fructus Piperis Longi is purchased in medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Chinese yam polysaccharide 10g.kg
-1(being equivalent to contain the crude drug amount), D group Fructus Piperis Longi ethanol extraction mixes 10g.kg with piperine in 8: 5 ratios
1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, the degree of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract group chemotherapy dependency diarrhoea is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine by weight in 2-6: 3-6: 3-4: the 5-9 ratio is formed
Wherein Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration preparation method of extract is:
Be 1: 1 or 1-6 according to proportion compatibility: 1 other ratios are got Rhizoma Dioscoreae, Fructus Piperis Longi, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract;
Wherein the preparation method of Chinese yam polysaccharide is:
Get dried Rhizoma Dioscoreae powder, with 95% ethanol 70 ℃ of reflux, extract, 3 times, each 4h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add 4 times of volume of ethanol, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, ethanol extraction, extract add the water redissolution and are made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, use tap water and distill water dialysis respectively, add 4 times of volume ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used ethanol respectively, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide;
Wherein the preparation method of Fructus Piperis Longi ethanol extraction is:
Get Fructus Piperis Longi, drying, crushing screening with 7-10 times of soak with ethanol 12h, is crossed the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged the centrifugal 15min of 3000rpm, separation of supernatant, water bath method, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, that is:
Wherein the preparation method of piperine is:
The Fructus Piperis Longi granule is ground into 20 orders, with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged, and is evaporated to thick, concentrated solution is put cold place and was left standstill 1-2 days, separates out piperine, filter crude product, the crude product of drying is dissolved in the ether, and 10%KOH solution washing three times is collected ether layer, boil off ether and get the faint yellow solid powder, the gained powder is dissolved in the ethanol, and the activated carbon decolorizing recrystallization namely gets the piperine of the prismatic crystallization of monocline.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction and piperine is 6: 6: 4: 9.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract
Be 1: 1 or 1-6 according to proportion compatibility: 1 other ratios are got Rhizoma Dioscoreae, Fructus Piperis Longi, and 10 times of amount distilled water soak 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, silk filters, and preserves filtrate, and medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, adopt 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract;
(2) preparation of Chinese yam polysaccharide
Get dried Rhizoma Dioscoreae powder, with 95% ethanol 70 ℃ of reflux, extract, 3 times, each 4h, remove supernatant, collect filtering residue, filtering residue extracts 4 times at 45 ℃ with distilled water, each 3h collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add 4 times of volume of ethanol, 4 ℃ of placements are spent the night, and are centrifugal, collecting precipitation, ethanol extraction, extract add the water redissolution and are made into 5% solution, add 1/5 volume Sevag reagent, concuss, centrifugal, get supernatant, add 1/5 volume Sevag reagent, so repeat repeatedly, until no white intermediate layer precipitation, collect supernatant concentration, use tap water and distill water dialysis respectively, add 4 times of volume ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used ethanol respectively, acetone and ether washing precipitation, lyophilization obtains white Chinese yam polysaccharide;
(3) preparation of Fructus Piperis Longi ethanol extraction
Get Fructus Piperis Longi, drying is pulverized, and sieves, with 7-10 times of soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged the centrifugal 15min of 3000rpm, separation of supernatant, water bath method, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, namely;
(4) preparation of piperine
The Fructus Piperis Longi granule is ground into 20 orders, with 7-10 times of soak with ethanol 12h, crosses the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged, and is evaporated to thick, concentrated solution is put cold place and was left standstill 1-2 days, separates out piperine, filter crude product, the crude product of drying is dissolved in the ether, and 10%KOH solution washing three times is collected ether layer, boil off ether and get the faint yellow solid powder, the gained powder is dissolved in the ethanol, and the activated carbon decolorizing recrystallization namely gets the piperine of the prismatic crystallization of monocline;
(5) choose Rhizoma Dioscoreae Fructus Piperis Longi water boiling concentration extract, Chinese yam polysaccharide, Fructus Piperis Longi ethanol extraction in proportion and evenly mix with piperine, namely.
4. the preparation method of natural drug composition as claimed in claim 3, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310118664 CN103182016A (en) | 2013-03-27 | 2013-03-27 | Traditional chinese medicine composition and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201310118664 CN103182016A (en) | 2013-03-27 | 2013-03-27 | Traditional chinese medicine composition and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103182016A true CN103182016A (en) | 2013-07-03 |
Family
ID=48673631
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201310118664 Pending CN103182016A (en) | 2013-03-27 | 2013-03-27 | Traditional chinese medicine composition and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103182016A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316088A (en) * | 2013-07-04 | 2013-09-25 | 石强 | Natural pharmaceutical composition |
| CN103340971A (en) * | 2013-07-19 | 2013-10-09 | 石强 | Natural drug extract |
| CN103385830A (en) * | 2013-07-19 | 2013-11-13 | 石强 | Traditional Chinese medicine composition and its preparation method and use |
| CN103393765A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Natural pharmaceutical composition and preparation method thereof |
| CN103393561A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Traditional chinese medicine composition |
| CN103655731A (en) * | 2013-12-11 | 2014-03-26 | 张宗升 | Natural pharmaceutical composition |
| CN103655806A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition as well as preparation method and application thereof |
| CN103689319A (en) * | 2013-12-31 | 2014-04-02 | 陈慧婷 | Pepper yam dispersible tablet and preparation method thereof |
-
2013
- 2013-03-27 CN CN 201310118664 patent/CN103182016A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316088A (en) * | 2013-07-04 | 2013-09-25 | 石强 | Natural pharmaceutical composition |
| CN103340971A (en) * | 2013-07-19 | 2013-10-09 | 石强 | Natural drug extract |
| CN103385830A (en) * | 2013-07-19 | 2013-11-13 | 石强 | Traditional Chinese medicine composition and its preparation method and use |
| CN103393765A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Natural pharmaceutical composition and preparation method thereof |
| CN103393561A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Traditional chinese medicine composition |
| CN103655731A (en) * | 2013-12-11 | 2014-03-26 | 张宗升 | Natural pharmaceutical composition |
| CN103655806A (en) * | 2013-12-14 | 2014-03-26 | 张宗升 | Natural medicine composition as well as preparation method and application thereof |
| CN103689319A (en) * | 2013-12-31 | 2014-04-02 | 陈慧婷 | Pepper yam dispersible tablet and preparation method thereof |
| CN103689319B (en) * | 2013-12-31 | 2015-07-01 | 陈慧婷 | Pepper yam dispersible tablet and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103182016A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103191201A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103272016A (en) | Traditional chinese medicine composition | |
| CN103385830A (en) | Traditional Chinese medicine composition and its preparation method and use | |
| CN103202898A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103182006A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103202886B (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103223082A (en) | Extract product of traditional Chinese medicine | |
| CN103251771A (en) | Traditional chinese medicine composition | |
| CN103316190A (en) | Natural pharmaceutical composition | |
| CN103223084A (en) | Traditional chinese medicine composition | |
| CN103230456A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN103223085A (en) | Chinese medicinal extract | |
| CN103349680A (en) | Natural pharmaceutical extractive | |
| CN103263492A (en) | Traditional chinese medicine extract | |
| CN103230465A (en) | Traditional chinese medicine extract | |
| CN103230496A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN103223040A (en) | Traditional chinese medicine composition | |
| CN103272097A (en) | Traditional chinese medicine extract | |
| CN103263503A (en) | Traditional chinese medicine extract | |
| CN103316191A (en) | Natural medicine extract | |
| CN103316186A (en) | Traditional chinese medicine composition | |
| CN103316189A (en) | Natural pharmaceutical composition | |
| CN103223050A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN103263472A (en) | Traditional chinese medicine extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130703 |