CN103340970A - Traditional chinese medicine composition - Google Patents
Traditional chinese medicine composition Download PDFInfo
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- CN103340970A CN103340970A CN2013103038805A CN201310303880A CN103340970A CN 103340970 A CN103340970 A CN 103340970A CN 2013103038805 A CN2013103038805 A CN 2013103038805A CN 201310303880 A CN201310303880 A CN 201310303880A CN 103340970 A CN103340970 A CN 103340970A
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- cortex phellodendri
- fructus crataegi
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- ethyl acetate
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Abstract
The invention relates to a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises a golden cypress and hawthorn decoction concentration extractive, a golden cypress and ethyl acetate extractive and hawthorn general flavone in a proportion of (5-8): (2-5): (3-7). The traditional Chinese medicine composition has a very good curative effect on cancer chemotherapy related diarrhea and meanwhile has a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition of being made up of Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones, the preparation method of this Chinese medicine composition and this Chinese medicine composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic no matter singly with or unite with all causing toxicities such as patient, diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but at present still unmatchful anticancer disease chemotherapy have the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Cortex Phellodendri is the dry bark of rutaceae wampee or Cortex Phellodendri, the energy heat and toxic materials clearing away medicine; Heat clearing and dampness removing medicine.Cure mainly: hygropyretic dysentery, have loose bowels.Fructus Crataegi another name Fructus Pyri Pashiae fruit, Rhododendron simsii Planch. fruit, it is long-pending to help digestion; Change the stasis of blood that stagnates.Cure mainly eating accumulation, dysentery.In recent years, Cortex Phellodendri and Fructus Crataegi antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, it is the compositions of novelty, and proved that it has fabulous curative effect and can finish the present invention thus simultaneously to chemotherapeutic sensitivity cancer chemotherapy dependency diarrhoea.
Summary of the invention
A kind of Chinese medicine composition provided by the invention is made of in the ratio of 5-8:2-5:3-7 by weight Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones is 8:5:7.
Wherein, Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Cortex Phellodendri Fructus Crataegi water boiling concentration preparation method of extract is in the above-mentioned Chinese medicine composition:
Be to get Cortex Phellodendri, Fructus Crataegi at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely.
The preparation method of Cortex Phellodendri ethyl acetate extract is in the above-mentioned Chinese medicine composition:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract.
The Fructus Crataegi total flavones preparation method is in the above-mentioned Chinese medicine composition:
Get haw pulp, with 60% alcohol reflux 2 times, h l.5 at every turn, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
The present invention also provides the preferred manufacturing procedure of this Chinese medicine composition, comprising:
(1) preparation of Cortex Phellodendri Fructus Crataegi water boiling concentration extract
Be to get Cortex Phellodendri, Fructus Crataegi at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely.
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract.
(3) Fructus Crataegi total flavones preparation
Get haw pulp, with 60% alcohol reflux 2 times, h l.5 at every turn, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
(4) choose Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones in proportion, evenly mix, namely.
The preparation method of above-mentioned Chinese medicine composition also comprises: the product that step (4) makes is further made the apsule and tablet that contains Chinese medicine composition 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of Chinese medicine composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, Chinese medicine composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this Chinese medicine composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this Chinese medicine composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this Chinese medicine composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor makes up Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 5-8:2-5:3-7, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 8:5:7, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this Chinese medicine composition Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and each component of Fructus Crataegi total flavones all can obtain by conventional means, some of them component for example Fructus Crataegi total flavones also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of Chinese medicine composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Cortex Phellodendri Fructus Crataegi water boiling concentration extract
Be to get Cortex Phellodendri, Fructus Crataegi at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely.
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract.
(3) Fructus Crataegi total flavones preparation
Get haw pulp, with 60% alcohol reflux 2 times, h l.5 at every turn, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take by weighing Cortex Phellodendri Fructus Crataegi water boiling concentration extract 40mg, Cortex Phellodendri ethyl acetate extract 25mg and the Fructus Crataegi total flavones 35mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of Chinese medicine composition tablet of the present invention
Take by weighing Cortex Phellodendri Fructus Crataegi water boiling concentration extract 40mg, Cortex Phellodendri ethyl acetate extract 25mg and the Fructus Crataegi total flavones 35mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones is 8:5:7.Inventor's compositions that the multiple proportioning combination between 5-8:2-5:3-7 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the Chinese medicine composition in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of traditional Chinese medicine composition for treating chemotherapy dependency diarrhoea effect
1, test material
(1) Cortex Phellodendri, Fructus Crataegi is purchased in medical material market.
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Cortex Phellodendri Fructus Crataegi water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Cortex Phellodendri ethyl acetate extract 10g.kg
-1(being equivalent to contain the crude drug amount), D group Fructus Crataegi total flavones 10g.kg
-1(being equivalent to contain the crude drug amount), the E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Chinese medicine composition group diarrhoea incidence rate of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of Chinese medicine composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group Chinese medicine composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5 ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Chinese medicine composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 Chinese medicine composition of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 32.5 |
| Compositions is low+cyclophosphamide | 38.5 |
| Height+cyclophosphamide | 47.1 |
Claims (5)
1. Chinese medicine composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition is made of in the ratio of 5-8:2-5:3-7 by weight Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones
Wherein Cortex Phellodendri Fructus Crataegi water boiling concentration preparation method of extract is:
Be to get Cortex Phellodendri, Fructus Crataegi at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely;
Wherein the preparation method of Cortex Phellodendri ethyl acetate extract is:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain;
Wherein the preparation method of Fructus Crataegi total flavones is:
Get haw pulp, with 60% alcohol reflux 2 times, h l.5 at every turn, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely.
2. Chinese medicine composition as claimed in claim 1 is characterized in that wherein the usage ratio of Cortex Phellodendri Fructus Crataegi water boiling concentration extract, Cortex Phellodendri ethyl acetate extract and Fructus Crataegi total flavones is 8:5:7.
3. as the preparation method of the Chinese medicine composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Cortex Phellodendri Fructus Crataegi water boiling concentration extract
Be to get Cortex Phellodendri, Fructus Crataegi at 1: 1 according to proportion compatibility, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating concentrates, ultrafiltration membrance filter, drying under reduced pressure, namely;
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain;
(3) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, h l.5 at every turn, merge extractive liquid, reclaims ethanol, gets extractum, and extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, namely;
(4) choose Cortex Phellodendri Fructus Crataegi water boiling concentration extract in proportion, the Cortex Phellodendri ethyl acetate extract evenly mixes with Fructus Crataegi total flavones, namely.
4. the preparation method of Chinese medicine composition as claimed in claim 3 is characterized in that also comprising: the product that step (4) makes is further made capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification by the pharmacy means of routine.
5. as the purposes of each described Chinese medicine composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655790A (en) * | 2013-12-10 | 2014-03-26 | 张宗升 | Natural pharmaceutical composition, and preparation method thereof |
| CN103690634A (en) * | 2013-12-10 | 2014-04-02 | 张宗升 | Natural pharmaceutical composition and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103202898A (en) * | 2013-03-29 | 2013-07-17 | 阚兆云 | Traditional chinese medicine composition and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103202898A (en) * | 2013-03-29 | 2013-07-17 | 阚兆云 | Traditional chinese medicine composition and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王跃华等: "川黄柏离体培养及药用成分的抑菌试验研究", 《中国中药杂志》, vol. 29, no. 10, 31 October 2004 (2004-10-31), pages 1002 - 1003 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655790A (en) * | 2013-12-10 | 2014-03-26 | 张宗升 | Natural pharmaceutical composition, and preparation method thereof |
| CN103690634A (en) * | 2013-12-10 | 2014-04-02 | 张宗升 | Natural pharmaceutical composition and preparation method thereof |
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Application publication date: 20131009 |