CN103191246A - Traditional chinese medicine extract and preparation method thereof - Google Patents
Traditional chinese medicine extract and preparation method thereof Download PDFInfo
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- CN103191246A CN103191246A CN 201310120675 CN201310120675A CN103191246A CN 103191246 A CN103191246 A CN 103191246A CN 201310120675 CN201310120675 CN 201310120675 CN 201310120675 A CN201310120675 A CN 201310120675A CN 103191246 A CN103191246 A CN 103191246A
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- radix scutellariae
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Abstract
The invention relates to a natural pharmaceutical composition, a preparation method thereof and application thereof in preparation of toxicity reducing and efficacy enhancing drugs for cancer chemotherapy. The natural pharmaceutical composition consists of a decoction concentrated extract of phellodendron bark and scutellaria root, an ethyl acetate extract of phellodendron bark, scutellaria root total flavonoids and baicalin which are in the ratio of (2-4):(2-8):(3-6):(5-7). The composition has an excellent treatment effect on cancer-chemotherapy-related diarrhea and can simultaneously play a synergic role in cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin, the preparation method of this natural drug composition and this natural drug composition are for the preparation of reducing cancer chemotherapy dependency diarrhoea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic no matter singly with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan etc. in recent years, the incidence rate of chemotherapy dependency diarrhoea improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of chemotherapy dependency diarrhoea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but at present still unmatchful anticancer disease chemotherapy have the Chinese patent medicine of alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Cortex Phellodendri is the dry bark of rutaceae wampee or Cortex Phellodendri, the energy heat and toxic materials clearing away medicine; Heat clearing and dampness removing medicine.Cure mainly: hygropyretic dysentery, have loose bowels.Radix Scutellariae is the root of labiate Huang, cure mainly dysentery, in recent years, Cortex Phellodendri and Radix Scutellariae antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained optimal drug ratio compositions of the present invention at last, and proved that it has fabulous curative effect and can play potentiation to treatment of cancer simultaneously cancer chemotherapy dependency diarrhoea, has finished the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, by Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin by weight in 2-4: the ratio of 2-8: 3-6: 5-7 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin is 4: 8: 6: 7.
Wherein, Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones can be by well known to a person skilled in the art the conventional method acquisition of purifying, and adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Cortex Phellodendri Radix Scutellariae water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Cortex Phellodendri, Radix Scutellariae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
The preparation method of Cortex Phellodendri ethyl acetate extract is in the above-mentioned natural drug composition:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract.
The Radix Scutellariae total flavones preparation method is in the above-mentioned natural drug composition:
Take by weighing the Radix Scutellariae coarse powder, place round-bottomed flask, with 55% ethanol 85 ℃ of following reflux, extract, repeatedly, filter, merging filtrate reclaims ethanol under Rotary Evaporators, concentrated, dry, obtains Radix Scutellariae total flavones.
The baicalin preparation method is in the above-mentioned natural drug composition:
The Radix Scutellariae oven dry, crushing screening takes by weighing in right amount, extracts at twice with 10 times, 8 times amounts with 80% ethanol, and each 1h filters.Merging filtrate reclaims ethanol, adds an amount of distilled water, adds concentrated hydrochloric acid and transfers pH3-4, leaves standstill 1h after-filtration decontamination, and filtrate is in 40 ℃ of accent pH1.5-2, and insulation 30min leaves standstill, sucking filtration, and precipitation is washed to pH6.5-7.0, is drying to obtain baicalin.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Cortex Phellodendri Radix Scutellariae water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Cortex Phellodendri, Radix Scutellariae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely.
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract.
(3) Radix Scutellariae total flavones preparation
Take by weighing the Radix Scutellariae coarse powder, place round-bottomed flask, with 55% ethanol 85 ℃ of following reflux, extract, repeatedly, filter, merging filtrate reclaims ethanol under Rotary Evaporators, concentrated, dry, obtains Radix Scutellariae total flavones.
(4) baicalin preparation
The Radix Scutellariae oven dry, crushing screening takes by weighing in right amount, extracts at twice with 10 times, 8 times amounts with 80% ethanol, and each 1h filters.Merging filtrate reclaims ethanol, adds an amount of distilled water, adds concentrated hydrochloric acid and transfers pH3-4, leaves standstill 1h after-filtration decontamination, and filtrate is in 40 ℃ of accent pH1.5-2, and insulation 30min leaves standstill, sucking filtration, and precipitation is washed to pH6.5-7.0, is drying to obtain baicalin.
(5) choose Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin in proportion, evenly mix, namely.
The preparation method of above-mentioned natural drug composition, also comprise: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this natural drug composition can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor is originally with Cortex Phellodendri Radix Scutellariae water boiling concentration extract, the Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin make up, and by a large amount of consumption proportions of determining these components of testing, make said composition bring into play good synergism, pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 2-4: 2-8: 3-6: produce synergism during 5-7, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio in each component is 4: 8: 6: 7 o'clock especially obvious, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin all can obtain by conventional means, wherein component such as Cortex Phellodendri ethyl acetate extract also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Cortex Phellodendri Radix Scutellariae water boiling concentration extract
Be 1: 1 or 1-4 according to proportion compatibility: 1 other ratios are got Cortex Phellodendri, Radix Scutellariae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, be.
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract.
(3) Radix Scutellariae total flavones preparation
Take by weighing the Radix Scutellariae coarse powder, place round-bottomed flask, with 55% ethanol 85 ℃ of following reflux, extract, repeatedly, filter, merging filtrate reclaims ethanol under Rotary Evaporators, concentrated, dry, obtains Radix Scutellariae total flavones.
(4) baicalin preparation
The Radix Scutellariae oven dry, crushing screening takes by weighing in right amount, extracts at twice with 10 times, 8 times amounts with 80% ethanol, and each 1h filters.Merging filtrate reclaims ethanol, adds an amount of distilled water, adds concentrated hydrochloric acid and transfers pH3-4, leaves standstill 1h after-filtration decontamination, and filtrate is in 40 ℃ of accent pH1.5-2, and insulation 30min leaves standstill, sucking filtration, and precipitation is washed to pH6.5-7.0, is drying to obtain baicalin.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Cortex Phellodendri Radix Scutellariae water boiling concentration extract 16mg, Cortex Phellodendri ethyl acetate extract 32mg, Radix Scutellariae total flavones 24mg and the baicalin 28mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Cortex Phellodendri Radix Scutellariae water boiling concentration extract 16mg, Cortex Phellodendri ethyl acetate extract 32mg, Radix Scutellariae total flavones 24mg and the baicalin 28mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin is 4: 8: 6: 7.The inventor to above-mentioned each composition usage ratio at 2-4: 2-8: 3-6: the compositions that the multiple proportioning combination in the time of between the 5-7 obtains has been carried out and last identical experiment, the natural drug composition of discovery in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Cortex Phellodendri, Radix Scutellariae is purchased in medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Cortex Phellodendri Radix Scutellariae water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Cortex Phellodendri ethyl acetate extract 10g.kg
-1(being equivalent to contain the crude drug amount), D group Radix Scutellariae total flavones mixes 10g.kg with baicalin in 6: 7 ratios
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, the degree of Cortex Phellodendri Radix Scutellariae water boiling concentration extract group chemotherapy dependency diarrhoea is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin by weight in 2-4: the ratio of 2-8: 3-6: 5-7 is formed
Wherein Cortex Phellodendri Radix Scutellariae water boiling concentration preparation method of extract is:
Be 1-4 according to proportion compatibility: 1 gets Cortex Phellodendri, Radix Scutellariae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely;
Wherein the preparation method of Cortex Phellodendri ethyl acetate extract is:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract;
Wherein the preparation method of Radix Scutellariae total flavones is:
Take by weighing the Radix Scutellariae coarse powder, place round-bottomed flask, with 55% ethanol 85 ℃ of following reflux, extract, repeatedly, filter, merging filtrate reclaims ethanol under Rotary Evaporators, concentrated, dry, obtains Radix Scutellariae total flavones;
Wherein the preparation method of baicalin is:
The Radix Scutellariae oven dry, crushing screening takes by weighing an amount of, extract at twice with 10 times, 8 times amounts with 80% ethanol, each 1h filters, merging filtrate reclaims ethanol, adds an amount of distilled water, add concentrated hydrochloric acid and transfer pH3-4, leave standstill 1h after-filtration decontamination, filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min leaves standstill sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain baicalin.
2. natural drug composition as claimed in claim 1 is characterized in that wherein the usage ratio of Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones and baicalin is 4: 8: 6: 7.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Cortex Phellodendri Radix Scutellariae water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Cortex Phellodendri, Radix Scutellariae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, namely;
(2) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, water, ether and ethyl acetate extraction successively after the aqueous dispersion, the distilling under reduced pressure of ethyl acetate part is drying to obtain the Cortex Phellodendri ethyl acetate extract;
(3) preparation of Radix Scutellariae total flavones
Take by weighing the Radix Scutellariae coarse powder, place round-bottomed flask, with 55% ethanol 85 ℃ of following reflux, extract, repeatedly, filter, merging filtrate reclaims ethanol under Rotary Evaporators, concentrated, dry, obtains Radix Scutellariae total flavones ketone;
(4) preparation of baicalin
The Radix Scutellariae oven dry, crushing screening takes by weighing an amount of, extract at twice with 10 times, 8 times amounts with 80% ethanol, each 1h filters, merging filtrate reclaims ethanol, adds an amount of distilled water, add concentrated hydrochloric acid and transfer pH3-4, leave standstill 1h after-filtration decontamination, filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min leaves standstill sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain baicalin;
(5) choose Cortex Phellodendri Radix Scutellariae water boiling concentration extract, Cortex Phellodendri ethyl acetate extract, Radix Scutellariae total flavones in proportion and evenly mix with baicalin, namely.
4. the preparation method of natural drug composition as claimed in claim 3, it is characterized in that also comprising: the product that step (5) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340937A (en) * | 2013-07-13 | 2013-10-09 | 石强 | Natural medicinal composition |
| CN103349697A (en) * | 2013-07-19 | 2013-10-16 | 石强 | Natural medicine extractive and preparation method and uses thereof |
| CN103349698A (en) * | 2013-07-20 | 2013-10-16 | 石强 | Natural pharmaceutical extractive, and preparation method thereof |
| CN103655706A (en) * | 2013-12-10 | 2014-03-26 | 张宗升 | Natural pharmaceutical composition, and preparation method and application thereof |
| CN103690604A (en) * | 2013-12-12 | 2014-04-02 | 张宗升 | Natural pharmaceutical composition and preparation method thereof |
| CN103690634A (en) * | 2013-12-10 | 2014-04-02 | 张宗升 | Natural pharmaceutical composition and preparation method thereof |
-
2013
- 2013-03-29 CN CN 201310120675 patent/CN103191246A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340937A (en) * | 2013-07-13 | 2013-10-09 | 石强 | Natural medicinal composition |
| CN103349697A (en) * | 2013-07-19 | 2013-10-16 | 石强 | Natural medicine extractive and preparation method and uses thereof |
| CN103349698A (en) * | 2013-07-20 | 2013-10-16 | 石强 | Natural pharmaceutical extractive, and preparation method thereof |
| CN103655706A (en) * | 2013-12-10 | 2014-03-26 | 张宗升 | Natural pharmaceutical composition, and preparation method and application thereof |
| CN103690634A (en) * | 2013-12-10 | 2014-04-02 | 张宗升 | Natural pharmaceutical composition and preparation method thereof |
| CN103690604A (en) * | 2013-12-12 | 2014-04-02 | 张宗升 | Natural pharmaceutical composition and preparation method thereof |
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Application publication date: 20130710 |