CN103690634A - Natural pharmaceutical composition and preparation method thereof - Google Patents
Natural pharmaceutical composition and preparation method thereof Download PDFInfo
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- CN103690634A CN103690634A CN201310663556.4A CN201310663556A CN103690634A CN 103690634 A CN103690634 A CN 103690634A CN 201310663556 A CN201310663556 A CN 201310663556A CN 103690634 A CN103690634 A CN 103690634A
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Abstract
The invention relates to a natural pharmaceutical composition and a preparation method thereof. The natural pharmaceutical composition is composed of a hawthorn-scutellaria baicalensis decoction concentrate extract, hawthorn total flavonoids, scutellaria baicalensis total flavonoids and baicalin in the ratio of (2-7): (3-5): (1-6): (3-7); the composition has good curative rate on cancer chemotherapy-associated diarrhea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being comprised of Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin, the preparation method of this natural drug composition and this natural drug composition are in the purposes for the preparation of reducing in cancer chemotherapy induced diarrhea medicine.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Fructus Crataegi another name Fructus Pyri Pashiae fruit, Rhododendron simsii Planch. fruit, can help digestion long-pending; Change the stagnant stasis of blood.Cure mainly eating accumulation, dysentery.Radix Scutellariae another name Radix Scutellariae, tsuchikane tea root, for labiate Radix Scutellariae (Scutellaria baicalensis Georgi), with root, be used as medicine, cure mainly dysentery, the inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and proved that it has fabulous curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 2-7:3-5:1-6:3-7 by weight Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin.
Above-mentioned natural drug composition, wherein the usage ratio of Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin is 7:5:6:7.
Wherein, Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Fructus Crataegi Radix Scutellariae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Fructus Crataegi, Radix Scutellariae, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of Fructus Crataegi total flavones is:
Get haw pulp, with 60% alcohol reflux 2 times, merge extractive liquid,, reclaim ethanol, obtain extractum, extractum adds appropriate distilled water, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, with ammonia, adjust pH to 7～8, add in macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water, wash away impurity, take ethanol as mobile phase desorption, reclaim ethanol, obtain.
In above-mentioned natural drug composition, Radix Scutellariae total flavones preparation method is:
Get Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 ℃ repeatedly, is filtered, and merging filtrate, reclaims ethanol, concentrated, dry, obtains.
In above-mentioned natural drug composition, baicalin preparation method is:
Radix Scutellariae pulverizes and sieves, and with 80% ethanol, extracts at twice, filters, and merging filtrate, reclaims ethanol, add appropriate distilled water, add concentrated hydrochloric acid and adjust pH3-4, elimination impurity after standing 1h, filtrate is adjusted pH1.5-2, insulation 30min in 40 ℃, standing, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Fructus Crataegi Radix Scutellariae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Fructus Crataegi, Radix Scutellariae, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, merge extractive liquid,, reclaim ethanol, obtain extractum, extractum adds appropriate distilled water, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, with ammonia, adjust pH to 7～8, add in macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water, wash away impurity, take ethanol as mobile phase desorption, reclaim ethanol, obtain.
(3) Radix Scutellariae total flavones preparation
Get Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 ℃ repeatedly, is filtered, and merging filtrate, reclaims ethanol, concentrated, dry, obtains.
(4) baicalin preparation
Radix Scutellariae pulverizes and sieves, and with 80% ethanol, extracts at twice, filters, and merging filtrate, reclaims ethanol, add appropriate distilled water, add concentrated hydrochloric acid and adjust pH3-4, elimination impurity after standing 1h, filtrate is adjusted pH1.5-2, insulation 30min in 40 ℃, standing, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
(5) choose in proportion Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
The beneficial effect of natural drug composition of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-7:3-5:1-6:3-7, potentiation is obvious, especially especially obvious when the usage ratio of each component is 7:5:6:7, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and each component of baicalin all can obtain by conventional means, component also can be buied by business as Fructus Crataegi total flavones, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of natural drug composition of the present invention, with and at the beneficial effect of anti-Chemotherapy-induced Diarrhea.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Crataegi Radix Scutellariae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Fructus Crataegi, Radix Scutellariae, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, merge extractive liquid,, reclaim ethanol, obtain extractum, extractum adds appropriate distilled water, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, with ammonia, adjust pH to 7～8, add in macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water, wash away impurity, take ethanol as mobile phase desorption, reclaim ethanol, obtain.
(3) Radix Scutellariae total flavones preparation
Get Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 ℃ repeatedly, is filtered, and merging filtrate, reclaims ethanol, concentrated, dry, obtains.
(4) baicalin preparation
Radix Scutellariae pulverizes and sieves, and with 80% ethanol, extracts at twice, filters, and merging filtrate, reclaims ethanol, add appropriate distilled water, add concentrated hydrochloric acid and adjust pH3-4, elimination impurity after standing 1h, filtrate is adjusted pH1.5-2, insulation 30min in 40 ℃, standing, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Fructus Crataegi Radix Scutellariae water boiling concentration extract 28mg, Fructus Crataegi total flavones 20mg, Radix Scutellariae total flavones 24mg and baicalin 28mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Fructus Crataegi Radix Scutellariae water boiling concentration extract 28mg, Fructus Crataegi total flavones 20mg, Radix Scutellariae total flavones 24mg and baicalin 28mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix with 5mg magnesium stearate, be pressed into tablet.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin is 7:5:6:7.Inventor's compositions that the multiple proportioning combination between 2-7:3-5:1-6:3-7 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Fructus Crataegi, Radix Scutellariae, is purchased from medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Fructus Crataegi Radix Scutellariae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Fructus Crataegi total flavones 10g.kg
-1(being equivalent to containing crude drug amount), D group Radix Scutellariae total flavones mixes 10g.kg with baicalin in 6:7 ratio
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-7:3-5:1-6:3-7 by weight Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin,
Wherein the preparation method of Fructus Crataegi Radix Scutellariae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Fructus Crataegi, Radix Scutellariae, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Fructus Crataegi total flavones is:
Get haw pulp, with 60% alcohol reflux 2 times, merge extractive liquid,, reclaim ethanol, obtain extractum, extractum adds appropriate distilled water, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, with ammonia, adjust pH to 7～8, add in macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water, wash away impurity, take ethanol as mobile phase desorption, reclaim ethanol, obtain;
Wherein the preparation method of Radix Scutellariae total flavones is:
Get Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 ℃ repeatedly, is filtered, and merging filtrate, reclaims ethanol, concentrated, dry, obtains;
Wherein the preparation method of baicalin is:
Radix Scutellariae pulverizes and sieves, and with 80% ethanol, extracts at twice, filters, and merging filtrate, reclaims ethanol, add appropriate distilled water, add concentrated hydrochloric acid and adjust pH3-4, elimination impurity after standing 1h, filtrate is adjusted pH1.5-2, insulation 30min in 40 ℃, standing, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and baicalin is 7:5:6:7.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Fructus Crataegi Radix Scutellariae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Fructus Crataegi, Radix Scutellariae, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, merge extractive liquid,, reclaim ethanol, obtain extractum, extractum adds appropriate distilled water, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, with ammonia, adjust pH to 7～8, add in macroporous adsorptive resins, regulate flow velocity 5 mL/min, with distilled water, wash away impurity, take ethanol as mobile phase desorption, reclaim ethanol, obtain;
(3) preparation of Radix Scutellariae total flavones
Get Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 ℃ repeatedly, is filtered, and merging filtrate, reclaims ethanol, concentrated, dry, obtains;
(4) preparation of baicalin
Radix Scutellariae pulverizes and sieves, and with 80% ethanol, extracts at twice, filters, and merging filtrate, reclaims ethanol, add appropriate distilled water, add concentrated hydrochloric acid and adjust pH3-4, elimination impurity after standing 1h, filtrate is adjusted pH1.5-2, insulation 30min in 40 ℃, standing, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain;
(5) choose in proportion Fructus Crataegi Radix Scutellariae water boiling concentration extract, Fructus Crataegi total flavones, Radix Scutellariae total flavones and evenly mix with baicalin, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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| CN104173330A (en) * | 2014-08-29 | 2014-12-03 | 上海市第六人民医院 | Medicine for preventing and treating cachexia and application of medicine |
| CN104996863A (en) * | 2015-06-17 | 2015-10-28 | 蚌埠市天星树脂有限责任公司 | Resin used for extracting original drugs of diarrhea relieving medicine and preparation method of resin |
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| CN104996863A (en) * | 2015-06-17 | 2015-10-28 | 蚌埠市天星树脂有限责任公司 | Resin used for extracting original drugs of diarrhea relieving medicine and preparation method of resin |
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Application publication date: 20140402 |