CN103655730A - Natural pharmaceutical composition - Google Patents
Natural pharmaceutical composition Download PDFInfo
- Publication number
- CN103655730A CN103655730A CN201310664278.4A CN201310664278A CN103655730A CN 103655730 A CN103655730 A CN 103655730A CN 201310664278 A CN201310664278 A CN 201310664278A CN 103655730 A CN103655730 A CN 103655730A
- Authority
- CN
- China
- Prior art keywords
- fructus piperis
- piperis longi
- radix scutellariae
- preparation
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a natural pharmaceutical composition. The natural pharmaceutical composition is prepared from a scutellaria baicalensis and fructus piperis longi decocted and concentrated extractive, total flavonoid of scutellaria baicalensis, a fructus piperis longi and alcohol extractive and piperine according to a ratio of (2-4): (6-8): (1-4): (3-9). The natural pharmaceutical composition has a curative effect on cancer chemotherapy related diarrhea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being comprised of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine, the preparation method of this natural drug composition and this natural drug composition are in the purposes for the preparation of reducing in cancer chemotherapy induced diarrhea medicine.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Radix Scutellariae another name Radix Scutellariae, tsuchikane tea root, for labiate Radix Scutellariae (Scutellaria baicalensis Georgi), be used as medicine with root, cures mainly dysentery.Fructus Piperis Longi is the green fruit of Piperaceae plant Piper longumI., warming spleen and stomach for dispelling cold, therapeutic method to keep the adverse QI flowing downwards pain relieving.Cure mainly: for coldness and pain in the epigastrium, the disease such as vomit, have loose bowels, the inventor is through research and the experiment of each a large amount of formula proportion, finally obtained optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can play the effect that suppresses tumor simultaneously cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 2-4:6-8:1-4:3-9 by weight Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine.
Above-mentioned natural drug composition, wherein the usage ratio of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine is 4:8:4:9.
Wherein, Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract is:
According to proportion compatibility, be 1-6: 1 gets Radix Scutellariae, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of Radix Scutellariae total flavones is:
Radix Scutellariae coarse powder, 55% alcohol reflux 2 times, filters, and merging filtrate reclaims ethanol, concentrated, dry, obtains.
In above-mentioned natural drug composition, Fructus Piperis Longi ethanol extraction preparation method is:
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
In above-mentioned natural drug composition, process for preparing piperine is:
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract
According to proportion compatibility, be 1-6: 1 gets Radix Scutellariae, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Radix Scutellariae total flavones
Radix Scutellariae coarse powder, 55% alcohol reflux 2 times, filters, and merging filtrate reclaims ethanol, concentrated, dry, obtains.
(3) Fructus Piperis Longi ethanol extraction preparation
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
(4) piperine preparation
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
(5) choose in proportion Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-4:6-8:1-4:3-9, potentiation is obvious, especially especially obvious when the usage ratio of each component is 4:8:4:9, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and each component of piperine all can obtain by conventional means, component also can be buied by business as piperine, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of natural drug composition of the present invention, with and the beneficial effect applied in diarrhea and antitumor.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract
According to proportion compatibility, be 1-6: 1 gets Radix Scutellariae, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Radix Scutellariae total flavones
Radix Scutellariae coarse powder, 55% alcohol reflux 2 times, filters, and merging filtrate reclaims ethanol, concentrated, dry, obtains.
(3) Fructus Piperis Longi ethanol extraction preparation
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
(4) piperine preparation
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Radix Scutellariae Fructus Piperis Longi water boiling concentration extract 16mg, Radix Scutellariae total flavones 32mg, Fructus Piperis Longi ethanol extraction 16mg and piperine 36mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Radix Scutellariae Fructus Piperis Longi water boiling concentration extract 16mg, Radix Scutellariae total flavones 32mg, Fructus Piperis Longi ethanol extraction 16mg and piperine 36mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix with 5mg magnesium stearate, be pressed into tablet.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine is 4:8:4:9.Inventor's compositions that the multiple proportioning combination between 2-4:6-8:1-4:3-9 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Radix Scutellariae, Fructus Piperis Longi, is purchased from medical material market.
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Radix Scutellariae Fructus Piperis Longi water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Radix Scutellariae total flavones 10g.kg
-1(being equivalent to containing crude drug amount), D group Fructus Piperis Longi ethanol extraction mixes 10g.kg with piperine in 4:9 ratio
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-4:6-8:1-4:3-9 by weight Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine,
Wherein the preparation method of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract is:
According to proportion compatibility, be 1-6: 1 gets Radix Scutellariae, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Radix Scutellariae total flavones is:
Radix Scutellariae coarse powder, 55% alcohol reflux 2 times, filters, and merging filtrate reclaims ethanol, concentrated, dry, obtains;
Wherein the preparation method of Fructus Piperis Longi ethanol extraction is:
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains;
Wherein the preparation method of piperine is:
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and piperine is 4:8:4:9.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Radix Scutellariae Fructus Piperis Longi water boiling concentration extract
According to proportion compatibility, be 1-6: 1 gets Radix Scutellariae, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Radix Scutellariae total flavones
Radix Scutellariae coarse powder, 55% alcohol reflux 2 times, filters, and merging filtrate reclaims ethanol, concentrated, dry, obtains;
(3) preparation of Fructus Piperis Longi ethanol extraction
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains;
(4) preparation of piperine
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains;
(5) choose in proportion Radix Scutellariae Fructus Piperis Longi water boiling concentration extract, Radix Scutellariae total flavones, Fructus Piperis Longi ethanol extraction and evenly mix with piperine, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprise: the product that step (5) is made further makes by conventional pharmacy means the capsule that contains natural drug composition 100mg or 200mg specification, maybe by the tablet of making by wet granulation technology containing natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310664278.4A CN103655730A (en) | 2013-12-11 | 2013-12-11 | Natural pharmaceutical composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310664278.4A CN103655730A (en) | 2013-12-11 | 2013-12-11 | Natural pharmaceutical composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103655730A true CN103655730A (en) | 2014-03-26 |
Family
ID=50295289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310664278.4A Pending CN103655730A (en) | 2013-12-11 | 2013-12-11 | Natural pharmaceutical composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103655730A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003206238A (en) * | 2001-11-06 | 2003-07-22 | Rohto Pharmaceut Co Ltd | Medicinal composition |
| CN103191201A (en) * | 2013-03-27 | 2013-07-10 | 张宗升 | Traditional chinese medicine composition and preparation method thereof |
| CN103263469A (en) * | 2013-04-16 | 2013-08-28 | 阚兆云 | Traditional Chinese medicine composition and preparation method thereof |
| CN103263503A (en) * | 2013-04-16 | 2013-08-28 | 阚兆云 | Traditional chinese medicine extract |
-
2013
- 2013-12-11 CN CN201310664278.4A patent/CN103655730A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003206238A (en) * | 2001-11-06 | 2003-07-22 | Rohto Pharmaceut Co Ltd | Medicinal composition |
| CN103191201A (en) * | 2013-03-27 | 2013-07-10 | 张宗升 | Traditional chinese medicine composition and preparation method thereof |
| CN103263469A (en) * | 2013-04-16 | 2013-08-28 | 阚兆云 | Traditional Chinese medicine composition and preparation method thereof |
| CN103263503A (en) * | 2013-04-16 | 2013-08-28 | 阚兆云 | Traditional chinese medicine extract |
Non-Patent Citations (1)
| Title |
|---|
| 田代华: "《实用中医对药方》", 31 March 2000, article "2064黄芩汤项下", pages: 885 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103202898B (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103385830A (en) | Traditional Chinese medicine composition and its preparation method and use | |
| CN103202886B (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103690634A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103239529A (en) | Traditional chinese medicine composition | |
| CN103223044A (en) | Traditional Chinese medicinal composition and its preparation method | |
| CN103251771A (en) | Traditional chinese medicine composition | |
| CN103223066A (en) | Chinese medicinal extract | |
| CN103230456A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN103655731A (en) | Natural pharmaceutical composition | |
| CN103655806A (en) | Natural medicine composition as well as preparation method and application thereof | |
| CN103655730A (en) | Natural pharmaceutical composition | |
| CN103656433A (en) | Natural pharmaceutical composition as well as preparation method and application thereof | |
| CN103656074A (en) | Traditional Chinese medicine composition | |
| CN103690665A (en) | Natural medicine composition | |
| CN103349697A (en) | Natural medicine extractive and preparation method and uses thereof | |
| CN103394014A (en) | Natural medicine composition | |
| CN103239534A (en) | Traditional chinese medicine composition | |
| CN103655808A (en) | Natural medicine composition and preparation method thereof | |
| CN103690604A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103655835A (en) | Natural pharmaceutical composition, and preparation method and application thereof | |
| CN103655977A (en) | Natural pharmaceutical composition | |
| CN103655774A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103655706A (en) | Natural pharmaceutical composition, and preparation method and application thereof | |
| CN103690603A (en) | Traditional Chinese medicinal composition, as well as preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140326 |