CN103656433A - Natural pharmaceutical composition as well as preparation method and application thereof - Google Patents
Natural pharmaceutical composition as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103656433A CN103656433A CN201310679124.2A CN201310679124A CN103656433A CN 103656433 A CN103656433 A CN 103656433A CN 201310679124 A CN201310679124 A CN 201310679124A CN 103656433 A CN103656433 A CN 103656433A
- Authority
- CN
- China
- Prior art keywords
- radix sanguisorbae
- semen alpiniae
- alpiniae katsumadai
- preparation
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a natural pharmaceutical composition as well as a preparation method and application thereof. The natural pharmaceutical composition comprises Alpinia katsumadai and sanguisorba officinalis decoction concentrated extract, Alpinia katsumadai ethyl acetate extract and sanguisorba total saponins in the proportion of (2-7):(3-6):(3-7). The composition has curative effect on cancer chemotherapy-induced diarrhea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being comprised of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin, the preparation method of this natural drug composition and this natural drug composition reduce the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Semen Alpiniae Katsumadai is the seed of zingiberaceous plant Semen Alpiniae Katsumadai Alpinia katsumadai Hayata.The < < Compendium of Materia Medica > > of Ming Dynasty's Li Shizhen (1518-1593 A.D.) supplements its effect for can " summer-heat that cures the wound is told lower leakage dysentery; dysphagia and regurgitation; feeling of fullness acid regurgitation " etc., the coldness and pain in the epigastrium of the cloudy stagnant taste of main cold-damp; Feeling of fullness is done swollen, vomiting; The disease such as have loose bowels.Radix Sanguisorbae is the dry root of rosaceous plant Radix Sanguisorbae Sanguisorba officinalis L. or Radix Sanguisorbae Sanguisorba officinalis L. var. longifolia (Bert.) the Y ü et Li that comes into leaves, the dysentery of stopping blooding, < < Japan hanako materia medica > > records Radix Sanguisorbae for blood disorders, dysentery with red and white feces watery diarrhea.The inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and has proved that it has good curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 2-7:3-6:3-7 by weight Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin.
Above-mentioned natural drug composition, wherein the usage ratio of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin is 7:6:7.
Wherein, Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract, can be by well known to a person skilled in the art the conventional method acquisition of purifying, the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of Semen Alpiniae Katsumadai ethyl acetate extract is:
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain.
In above-mentioned natural drug composition, radix sanguisorbae total saponin preparation method is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain.
(3) radix sanguisorbae total saponin preparation
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
(4) choose in proportion Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-7:3-6:3-7, potentiation is obvious, especially especially obvious when the usage ratio of each component is 7:6:7, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and each component of radix sanguisorbae total saponin all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of natural drug composition of the present invention, with and at the beneficial effect of anti-Chemotherapy-induced Diarrhea.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain.
(3) radix sanguisorbae total saponin preparation
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract 35mg, Semen Alpiniae Katsumadai ethyl acetate extract 30mg and radix sanguisorbae total saponin 35mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix filled capsules.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract 35mg, Semen Alpiniae Katsumadai ethyl acetate extract 30mg and radix sanguisorbae total saponin 35mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix tabletting with 5mg magnesium stearate.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin is 7:6:7.Inventor's compositions that the multiple proportioning combination between 2-7:3-6:3-7 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Semen Alpiniae Katsumadai, Radix Sanguisorbae
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Semen Alpiniae Katsumadai ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), D group is radix sanguisorbae total saponin 10g.kg
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-7:3-6:3-7 by weight Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin,
Wherein the preparation method of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract is:
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Semen Alpiniae Katsumadai ethyl acetate extract is:
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain;
Wherein the preparation method of radix sanguisorbae total saponin is:
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract and radix sanguisorbae total saponin is 7:6:7.
3. the preparation method of natural drug composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract
According to proportion compatibility, be 1-4: 1 gets Semen Alpiniae Katsumadai, Radix Sanguisorbae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Semen Alpiniae Katsumadai ethyl acetate extract
Dry Semen Alpiniae Katsumadai is pulverized, crossed 20-40 mesh sieve, by 70% industrial alcohol heating and refluxing extraction 3 times, each 2 h, decompression and solvent recovery obtains dry extract, extractum is diluted to after suspension with hot water, puts to room temperature, and ethyl acetate extraction, reclaims solvent, dry, pulverize, sieve, obtain;
(3) preparation of radix sanguisorbae total saponin
Dry Radix Sanguisorbae pulverizing medicinal materials, 70% soak with ethanol is spent the night, reflux 3 times, merge extractive liquid, reclaims to obtain ethanol extraction, and this ethanol extraction is dissolved in water, equal-volume n-butanol extraction 3 times, decompression and solvent recovery, vacuum drying and get final product;
(4) choose in proportion Semen Alpiniae Katsumadai Radix Sanguisorbae water boiling concentration extract, Semen Alpiniae Katsumadai ethyl acetate extract evenly mixes with radix sanguisorbae total saponin, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310679124.2A CN103656433A (en) | 2013-12-14 | 2013-12-14 | Natural pharmaceutical composition as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310679124.2A CN103656433A (en) | 2013-12-14 | 2013-12-14 | Natural pharmaceutical composition as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103656433A true CN103656433A (en) | 2014-03-26 |
Family
ID=50295991
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310679124.2A Pending CN103656433A (en) | 2013-12-14 | 2013-12-14 | Natural pharmaceutical composition as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103656433A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106723043A (en) * | 2017-01-04 | 2017-05-31 | 郭徽 | A kind of food compositions of three high drop |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103230466A (en) * | 2013-04-08 | 2013-08-07 | 张宗升 | Traditional Chinese medicine extract and preparation method thereof |
| CN103263482A (en) * | 2013-03-29 | 2013-08-28 | 阚兆云 | Extract from traditional Chinese medicine and its preparation method |
| CN103349681A (en) * | 2013-07-20 | 2013-10-16 | 石强 | Natural medicine composition |
| CN103356754A (en) * | 2013-07-19 | 2013-10-23 | 石强 | Chinese medicinal composition |
| CN103385830A (en) * | 2013-07-19 | 2013-11-13 | 石强 | Traditional Chinese medicine composition and its preparation method and use |
| CN103385922A (en) * | 2013-07-20 | 2013-11-13 | 石强 | Natural pharmaceutical composition and preparation method |
-
2013
- 2013-12-14 CN CN201310679124.2A patent/CN103656433A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103263482A (en) * | 2013-03-29 | 2013-08-28 | 阚兆云 | Extract from traditional Chinese medicine and its preparation method |
| CN103230466A (en) * | 2013-04-08 | 2013-08-07 | 张宗升 | Traditional Chinese medicine extract and preparation method thereof |
| CN103356754A (en) * | 2013-07-19 | 2013-10-23 | 石强 | Chinese medicinal composition |
| CN103385830A (en) * | 2013-07-19 | 2013-11-13 | 石强 | Traditional Chinese medicine composition and its preparation method and use |
| CN103349681A (en) * | 2013-07-20 | 2013-10-16 | 石强 | Natural medicine composition |
| CN103385922A (en) * | 2013-07-20 | 2013-11-13 | 石强 | Natural pharmaceutical composition and preparation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106723043A (en) * | 2017-01-04 | 2017-05-31 | 郭徽 | A kind of food compositions of three high drop |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103251706A (en) | Traditional chinese medicine composition | |
| CN103202886B (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103656433A (en) | Natural pharmaceutical composition as well as preparation method and application thereof | |
| CN103239528A (en) | Traditional chinese medicine composition | |
| CN103239529A (en) | Traditional chinese medicine composition | |
| CN103251771A (en) | Traditional chinese medicine composition | |
| CN103655806A (en) | Natural medicine composition as well as preparation method and application thereof | |
| CN103655731A (en) | Natural pharmaceutical composition | |
| CN103656074A (en) | Traditional Chinese medicine composition | |
| CN103394014A (en) | Natural medicine composition | |
| CN103393812A (en) | Traditional Chinese medicine composition and preparation method thereof | |
| CN103223136A (en) | Traditional Chinese medicine extract and its preparation method | |
| CN103655804A (en) | Traditional chinese medicine composition | |
| CN103223049A (en) | Chinese medicinal extract | |
| CN103239534A (en) | Traditional chinese medicine composition | |
| CN103655809A (en) | Natural composition and preparation method thereof | |
| CN103230559A (en) | Traditional Chinese medicine composition and preparation method thereof | |
| CN103656432A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103656427A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103656426A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103655808A (en) | Natural medicine composition and preparation method thereof | |
| CN103655807A (en) | Traditional Chinese medicine composition | |
| CN103690585A (en) | Natural pharmaceutical composition as well as preparation method and application thereof | |
| CN103655859A (en) | Natural pharmaceutical composition | |
| CN103655799A (en) | Traditional chinese medicine composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140326 |