CN103655837A - Traditional chinese medicine composition - Google Patents
Traditional chinese medicine composition Download PDFInfo
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- CN103655837A CN103655837A CN201310668109.8A CN201310668109A CN103655837A CN 103655837 A CN103655837 A CN 103655837A CN 201310668109 A CN201310668109 A CN 201310668109A CN 103655837 A CN103655837 A CN 103655837A
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- artemisiae argyi
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Abstract
The invention relates to a traditional Chinese medicine composition and a preparation method thereof, and application of the traditional Chinese medicine composition in preparation of attenuated synergistic medicines in cancer chemotherapy. The traditional Chinese medicine composition is composed of 2-9 parts of folium artemisiae argyi/ dried orange peel water decoction concentrated extract, 1-4 parts of folium artemisiae argyi ethyl acetate extract and 5-7 parts of nobiletin. The composition has curative effect on cancer chemotherapy related diarrhoea.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate more specifically to a kind of Chinese medicine composition being comprised of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin, the preparation method of this Chinese medicine composition and this Chinese medicine composition are in the purposes for the preparation of reducing in cancer chemotherapy induced diarrhea medicine.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Folium Artemisiae Argyi is one of tcm clinical practice common medicine, is the leaf of Compositae sagebruss Artemisiae argyi Led.etVant., and nature and flavor are bitter, pungent, warm, return liver,spleen,kidney warp, has the effects such as pain relieving, antiabortive, dysentery relieving, dissipating depression of QI.Pericarpium Citri Reticulatae, the mature peel for rutaceae orange (Citrus reticulata Blanco) and variety thereof, has another name called Pericarpium Citri tangerinae, for fullness in the epigastrium and chest, lack of appetite and vomiting, cough ant phlegm.The inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and has proved that it has fabulous curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of Chinese medicine composition provided by the invention, consists of in the ratio of 2-9:1-4:5-7 by weight Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin.
Above-mentioned Chinese medicine composition, wherein the usage ratio of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin is 9:4:7.
Wherein, Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned Chinese medicine composition, the preparation method of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract is:
According to proportion compatibility, be that 1-4:1 gets Folium Artemisiae Argyi, Pericarpium Citri Reticulatae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned Chinese medicine composition, the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains extractum, and extractum is with after normal hexane extraction, and ethyl acetate is extracted, and reclaims solvent and get final product.
In above-mentioned Chinese medicine composition, Nobiletin preparation method is:
Pericarpium Citri Reticulatae is pulverized, sieves, and petroleum ether reflux, extract, 2 times, merge extractive liquid,, reclaims after petroleum ether, with the mixed solvent heating for dissolving of 2 kinds of solvent compositions of 0.25 times of amount, place, to be crystallized separating out completely, after filtration, at 80 ℃, dry, be extracted with ethyl acetate, concentrated, polyamide column chromatography, with the mixed solvent eluting of 2 kinds of solvent compositions, in conjunction with thin layer chromatography, collect containing Nobiletin eluent and volatilize solvent, recrystallizing methanol, obtains.
The present invention also provides the preferred preparation method of this Chinese medicine composition, comprising:
(1) preparation of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract
According to proportion compatibility, be that 1-4:1 gets Folium Artemisiae Argyi, Pericarpium Citri Reticulatae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains extractum, and extractum is with after normal hexane extraction, and ethyl acetate is extracted, and reclaims solvent and get final product.
(3) Nobiletin preparation
Pericarpium Citri Reticulatae is pulverized, sieves, and petroleum ether reflux, extract, 2 times, merge extractive liquid,, reclaims after petroleum ether, with the mixed solvent heating for dissolving of 2 kinds of solvent compositions of 0.25 times of amount, place, to be crystallized separating out completely, after filtration, at 80 ℃, dry, be extracted with ethyl acetate, concentrated, polyamide column chromatography, with the mixed solvent eluting of 2 kinds of solvent compositions, in conjunction with thin layer chromatography, collect containing Nobiletin eluent and volatilize solvent, recrystallizing methanol, obtains.
(4) choose in proportion Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin, evenly mix, obtain.
The preparation method of above-mentioned Chinese medicine composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
Chinese medicine composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, Chinese medicine composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of Chinese medicine composition of the present invention has produced unexpected synergism when 2-9:1-4:5-7, potentiation is obvious, especially especially obvious when the usage ratio of each component is 9:4:7, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this Chinese medicine composition Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and each component of Nobiletin all can obtain by conventional means, Nobiletin also can be buied by business, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of Chinese medicine composition of the present invention, with and at the beneficial effect of anti-Chemotherapy-induced Diarrhea.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract
According to proportion compatibility, be that 1-4:1 gets Folium Artemisiae Argyi, Pericarpium Citri Reticulatae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains extractum, and extractum is with after normal hexane extraction, and ethyl acetate is extracted, and reclaims solvent and get final product.
(3) Nobiletin preparation
Pericarpium Citri Reticulatae is pulverized, sieves, and petroleum ether reflux, extract, 2 times, merge extractive liquid,, reclaims after petroleum ether, with the mixed solvent heating for dissolving of 2 kinds of solvent compositions of 0.25 times of amount, place, to be crystallized separating out completely, after filtration, at 80 ℃, dry, be extracted with ethyl acetate, concentrated, polyamide column chromatography, with the mixed solvent eluting of 2 kinds of solvent compositions, in conjunction with thin layer chromatography, collect containing Nobiletin eluent and volatilize solvent, recrystallizing methanol, obtains.
Second step: the preparation of preparation
(1) preparation of Chinese medicinal composition capsules preparation of the present invention
Take respectively Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract 45mg, Folium Artemisiae Argyi ethyl acetate extract 20mg and Nobiletin 35mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix filled capsules.
(2) preparation of Chinese medicine composition tablet of the present invention
Take respectively Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract 45mg, Folium Artemisiae Argyi ethyl acetate extract 20mg and Nobiletin 35mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix tabletting with 5mg magnesium stearate.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin is 9:4:7.Inventor's compositions that the multiple proportioning combination between 2-9:1-4:5-7 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the Chinese medicine composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example: the pharmacodynamic study of traditional Chinese medicine composition for treating Chemotherapy-induced Diarrhea effect
1, test material
(1) Folium Artemisiae Argyi, Pericarpium Citri Reticulatae, is purchased from medical material market.
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Folium Artemisiae Argyi ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), D group Nobiletin 10g.kg
-1(being equivalent to containing crude drug amount), E group is Chinese medicine composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, Chinese medicine composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a Chinese medicine composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-9:1-4:5-7 by weight Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin,
Wherein the preparation method of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract is:
According to proportion compatibility, be that 1-4:1 gets Folium Artemisiae Argyi, Pericarpium Citri Reticulatae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains extractum, and extractum is with after normal hexane extraction, and ethyl acetate is extracted, and reclaims solvent and get final product;
Wherein the preparation method of Nobiletin is:
Pericarpium Citri Reticulatae is pulverized, sieves, and petroleum ether reflux, extract, 2 times, merge extractive liquid,, reclaims after petroleum ether, with the mixed solvent heating for dissolving of 2 kinds of solvent compositions of 0.25 times of amount, place, to be crystallized separating out completely, after filtration, at 80 ℃, dry, be extracted with ethyl acetate, concentrated, polyamide column chromatography, with the mixed solvent eluting of 2 kinds of solvent compositions, in conjunction with thin layer chromatography, collect containing Nobiletin eluent and volatilize solvent, recrystallizing methanol, obtains.
2. Chinese medicine composition as claimed in claim 1, wherein the usage ratio of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract and Nobiletin is 9:4:7.
3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, is characterized in that the method comprises:
(1) preparation of Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract
According to proportion compatibility, be that 1-4:1 gets Folium Artemisiae Argyi, Pericarpium Citri Reticulatae, distilled water immersion, heating decocts, and filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains extractum, and extractum is with after normal hexane extraction, and ethyl acetate is extracted, and reclaims solvent and get final product;
(3) preparation of Nobiletin
Pericarpium Citri Reticulatae is pulverized, sieves, and petroleum ether reflux, extract, 2 times, merge extractive liquid,, reclaims after petroleum ether, with the mixed solvent heating for dissolving of 2 kinds of solvent compositions of 0.25 times of amount, place, to be crystallized separating out completely, after filtration, at 80 ℃, dry, be extracted with ethyl acetate, concentrated, polyamide column chromatography, with the mixed solvent eluting of 2 kinds of solvent compositions, in conjunction with thin layer chromatography, collect containing Nobiletin eluent and volatilize solvent, recrystallizing methanol, obtains;
(4) choose in proportion Folium Artemisiae Argyi Pericarpium Citri Reticulatae water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract evenly mixes with Nobiletin, obtain.
4. the preparation method of Chinese medicine composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains Chinese medicine composition 100mg or 200mg specification.
5. the Chinese medicine composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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| CN201310668109.8A CN103655837A (en) | 2013-12-11 | 2013-12-11 | Traditional chinese medicine composition |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008086282A (en) * | 2006-10-04 | 2008-04-17 | Nanto Shuhan Kk | Mugwort extract drink and method for improving preservability of the same |
| CN103181959A (en) * | 2013-03-27 | 2013-07-03 | 张宗升 | Traditional chinese medicine composition and preparation method thereof |
| CN103394014A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Natural medicine composition |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008086282A (en) * | 2006-10-04 | 2008-04-17 | Nanto Shuhan Kk | Mugwort extract drink and method for improving preservability of the same |
| CN103181959A (en) * | 2013-03-27 | 2013-07-03 | 张宗升 | Traditional chinese medicine composition and preparation method thereof |
| CN103394014A (en) * | 2013-07-20 | 2013-11-20 | 石强 | Natural medicine composition |
Non-Patent Citations (1)
| Title |
|---|
| 李亚: "《古典单方应用》", 31 May 2011, article "古典单方应用" * |
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Application publication date: 20140326 |