CN104940368B - A kind of medicine for preventing Nosema apis parasitosis and application thereof - Google Patents
A kind of medicine for preventing Nosema apis parasitosis and application thereof Download PDFInfo
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- CN104940368B CN104940368B CN201510302635.1A CN201510302635A CN104940368B CN 104940368 B CN104940368 B CN 104940368B CN 201510302635 A CN201510302635 A CN 201510302635A CN 104940368 B CN104940368 B CN 104940368B
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- honeybee
- nosema apis
- cortex phellodendri
- parasitosis
- microsporidian
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention provides a kind of natural plant extracts for preventing Nosema apis parasitosis, by by dry phellodendron bark, add water to submerge after standing at room temperature, it is fried, it is obtained by filtration Cortex Phellodendri water extract, then Cortex Phellodendri water extract syrup mixture Installed are configured in feeder by volume for 0.5% 3% with 50% 60% sucrose syrups, allow infection microsporidian the free feeding of honeybee, act on honeybee and microsporidian, thus reach prevention Nosema apis parasitosis effect.The present invention is good to the prevention effect of Nosema apis parasitosis under certain concentration range, to honeybee small toxicity;Medicine is derived from a wealth of sources, is easy to get, producing simply, and cost is low;The medicament residue problem to bee product and environment is not present in extract of the present invention, to people and animals and environment " no pollution ".It is the high effective way for preventing Nosema apis parasitosis of a kind of health, environmental protection, ecology.
Description
Technical field
The invention belongs to the protective agents of honeybee diseases in bee rearing management domain, are related to a kind of prevention Nosema apis
The medicine of parasitosis and its application.
Background technology
Nosema apis parasitosis, is that a kind of adult bee is normal as caused by Nosema apis worm also known as " nosema disease "
The infectious disease of the digestive tract seen.Nosema apis worm mainly by infecting adult bees intestinal epithelial cell, destroys honeybee enteron aisle
Normal stool function, so as to reduce the constitution of honeybee, shorten the service life of honeybee.When bee colony is diseased slight, symptom unobvious, often
It is often ignored, and it is once serious, or even the collapse of whole bee colony can be caused.The microsporidian of honeybee mainly has(West)Honeybee is micro-
Sporozoite(Nosema apis)With Eastern bee microsporidian(Nosema ceranae).Numerous studies find east in recent years
The many areas gradually substitution in the range of world's bee-keeping of Nosema apis worm(West)Nosema apis worm, it is complete to become harm
One of important disease of ball apiculture.We also indicate that in the investigation to Zhejiang Province Apis mellifera surely, by Eastern bee microsporidian
Caused Nosema apis parasitosis is widely current, and some areas are in a bad way.
Fumidil is the currently the only key agents that can effectively prevent Nosema apis parasitosis.But it is used as a kind of anti-
Raw element, long-time service of the fumidil in bee colony can cause the medicament residue problem of bee product.Existing numerous studies show cigarette
Aspergillin can produce health in the disease such as mankind's encephalitis microsporidiosis and cancer is treated certain side effect.Cause
This fumidil only allows registration to use in the minority bee-keeping countries such as the U.S., Canada at present, and European Union member countries, which then forbid using, is somebody's turn to do
Medicine.And almost past medical help in other bee-keeping countries and regions such as China, Nosema apis parasitosis.With the improvement of living standards,
Pursuit of the people to healthy, safe and pollution-free bee product is also higher and higher.Therefore develop micro- to can effectively prevent honeybee
Sporidiosis, it is comparatively safe to people and animals and environment, will not remaining natural plant extracts seem very necessary.
Cortex Phellodendri water extract is using Cortex Phellodendri bark as raw material, is first decocted after cold water soak by boiling slow boiling slightly boiling by intense fire
What system formed.Mainly contain a variety of alkaloids such as jamaicin, phellodendrine, phellodendrine.
The content of the invention
The object of the present invention is to provide a kind of natural plant extracts for preventing Nosema apis parasitosis.Pass through following steps
Realize:The phellodendron bark for taking a certain amount of drying at room temperature to cross, adds the water that mass volume ratio is 10-15 times, phellodendron bark is totally submerged,
After standing 2 h at room temperature, rear slow boiling slightly boiling is first boiled by intense fire, fried 40-60 min, take drug containing Aqueous extracts, add 10-15 times
Water, repeat fried 40-60 min, take drug containing Aqueous extracts, merge filtrate twice, both Cortex Phellodendri water extract, low temperature seal preserve.
It is a further object to provide the natural plant extracts to prepare prevention Nosema apis parasitosis
Application in middle medicine, the natural plant extracts are Cortex Phellodendri water extract.Realized by following steps:By Cortex Phellodendri water extract
It is that 50%-60% sucrose syrups are the Cortex Phellodendri water extract that 0.5%-3% is configured to various concentrations by volume with mass percent concentration
Syrup mixture , Installed in feeder, allow infection microsporidian the free feeding of honeybee, act on honeybee and microsporidian, from
And reach the effect of prevention Nosema apis parasitosis.
The method of the present invention has the characteristics that compared with existing medical treatment method:(1)In certain concentration range
Under, it is good to the prevention effect of Nosema apis parasitosis, to honeybee small toxicity;(2)Medicine is derived from a wealth of sources, is easy to get, producing simply,
Cost is low;(3)There is no the medicament residue problem to bee product and environment, to people and animals and environment " no pollution ".It is a kind of
Health, environmental protection, the high effective way for preventing Nosema apis parasitosis of ecology.
Embodiment
The present invention is further described in conjunction with the embodiments.
Embodiment 1
Phellodendron barks of 10 g after drying at room temperature is weighed, the cold water of 100 ml is added, Cortex Phellodendri medicinal material is totally submerged, room temperature
After 2 h of lower standing, after first boiling slow boiling slightly boiling 40-60 min again by intense fire, filtrate is filtered to take.Added again in the residue toward after filtering
The cold water of 100 ml, repeats above ultrasonic step, takes filtrate, merges filtrate twice, and low temperature seal preserves.Take a certain amount of Cortex Phellodendri
The sucrose syrup that water extract is 60% with mass percent concentration is configured to the Cortex Phellodendri water extract syrup mixture that volume ratio is 3%,
Allow infection microsporidian the free feeding of honeybee.
As a result:7th d and the 13rd d after infection, feeds the microsporidian spore in the infected honeybee body of medicated syrup
Number is feed the not infected honeybee of medicated syrup 42.5% and 30.1% respectively, and the 15th d, dispenser group are felt after infection
It is 60% to contaminate bee survival, and the infected bee survival without medicine feed group is 77%.
Embodiment 2
Phellodendron barks of 10 g after drying at room temperature is weighed, the cold water of 100 ml is added, Cortex Phellodendri medicinal material is totally submerged, room temperature
After 2 h of lower standing, after first boiling slow boiling slightly boiling 40-60 min again by intense fire, filtrate is filtered to take.Added again in the residue toward after filtering
The cold water of 100 ml, repeats above ultrasonic step, takes filtrate, merges filtrate twice, and low temperature seal preserves.Take a certain amount of Cortex Phellodendri
The sucrose syrup that water extract is 60% with mass percent concentration is configured to the Cortex Phellodendri water extract syrup mixture that volume ratio is 2%,
Allow infection microsporidian the free feeding of honeybee.
As a result:7th d and the 13rd d after infection, feeds the microsporidian spore in the infected honeybee body of medicated syrup
Number is feed the not infected honeybee of medicated syrup 40.3% and 23% respectively.15th d after infection, dispenser group it is infected
Bee survival is 69%, and the infected bee survival without medicine feed group is 77%.
Embodiment 3
Phellodendron barks of 10 g after drying at room temperature is weighed, the cold water of 100 ml is added, Cortex Phellodendri medicinal material is totally submerged, room temperature
After 2 h of lower standing, after first boiling slow boiling slightly boiling 40-60 min again by intense fire, filtrate is filtered to take.Added again in the residue toward after filtering
The cold water of 100 ml, repeats above ultrasonic step, takes filtrate, merges filtrate twice, and low temperature seal preserves.Take a certain amount of Cortex Phellodendri
The sucrose syrup that water extract is 60% with mass percent concentration is configured to the Cortex Phellodendri water extract syrup mixture that volume ratio is 1%,
Allow infection microsporidian the free feeding of honeybee.
As a result:7th d and the 13rd d after infection, feeds the microsporidian spore in the infected honeybee body of medicated syrup
Number is feed the not infected honeybee of medicated syrup 40.4% and 48.2% respectively.15th d after infection, dispenser group are felt
It is 89% to contaminate bee survival, and the infected bee survival without medicine feed group is 77%.
Embodiment 4
Phellodendron barks of 10 g after drying at room temperature is weighed, the cold water of 100 ml is added, Cortex Phellodendri medicinal material is totally submerged, room temperature
After 2 h of lower standing, after first boiling slow boiling slightly boiling 40-60 min again by intense fire, filtrate is filtered to take.Added again in the residue toward after filtering
The cold water of 100 ml, repeats above ultrasonic step, takes filtrate, merges filtrate twice, and low temperature seal preserves.Take a certain amount of Cortex Phellodendri
The sucrose syrup that water extract is 50% with mass percent concentration is configured to the Cortex Phellodendri water extract syrup mixture that volume ratio is 3%,
Allow infection microsporidian the free feeding of honeybee.
As a result:7th d and the 13rd d after infection, feeds the microsporidian spore in the infected honeybee body of medicated syrup
Number is feed the not infected honeybee of medicated syrup 40.0% and 35% respectively, after infection the 15th d, dispenser group it is infected
Bee survival is 65%, and the infected bee survival without medicine feed group is 77%.
Embodiment 5
Phellodendron barks of 10 g after drying at room temperature is weighed, the cold water of 100 ml is added, Cortex Phellodendri medicinal material is totally submerged, room temperature
After 2 h of lower standing, after first boiling slow boiling slightly boiling 40-60 min again by intense fire, filtrate is filtered to take.Added again in the residue toward after filtering
The cold water of 100 ml, repeats above ultrasonic step, takes filtrate, merges filtrate twice, and low temperature seal preserves.Take a certain amount of Cortex Phellodendri
Water extract is configured to the Cortex Phellodendri water extract syrup that volume ratio is 0.5% with the sucrose syrup that mass percent concentration is 60% and mixes
Thing, allow infection microsporidian the free feeding of honeybee.
As a result:7th d and the 13rd d after infection, feeds the microsporidian spore in the infected honeybee body of medicated syrup
Number is feed the not infected honeybee of medicated syrup 50% and 55% respectively.15th d after infection, the infected honey of dispenser group
Bee survival rate is 80%, and the infected bee survival without medicine feed group is 77%.
Claims (1)
1. a kind of composition for preventing Nosema apis parasitosis is preparing the application in preventing Nosema apis parasitosis medicine, it is special
Sign is that the composition is made of natural plant extracts and sucrose syrup, and the natural plant extracts are Cortex Phellodendri water
Extract, the composition are realized by following steps:It is 50%-60% sucrose syrups by Cortex Phellodendri water extract and mass percent concentration
Cortex Phellodendri water extract syrup composition is configured to for 0.5%-3% by volume, loaded in feeder, allowing the honey of infection microsporidian
The free feeding of bee, acts on honeybee and microsporidian, so as to reach the effect of prevention Nosema apis parasitosis.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102697934A (en) * | 2012-06-25 | 2012-10-03 | 陈慧婷 | Golden cypress traditional Chinese medicine preparation for treating diarrhea and preparation method for preparation |
CN103690665A (en) * | 2013-12-12 | 2014-04-02 | 张宗升 | Natural medicine composition |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102697934A (en) * | 2012-06-25 | 2012-10-03 | 陈慧婷 | Golden cypress traditional Chinese medicine preparation for treating diarrhea and preparation method for preparation |
CN103690665A (en) * | 2013-12-12 | 2014-04-02 | 张宗升 | Natural medicine composition |
Non-Patent Citations (1)
Title |
---|
黄柏及其炮制品水提物体内、外抑菌作用研究;林海等;《中国药房》;20121231;第23卷(第31期);第2900-2902页,尤其是第2900-2902页 * |
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