CN107280008A - A kind of dietary supplements and its preparation method and application - Google Patents

A kind of dietary supplements and its preparation method and application Download PDF

Info

Publication number
CN107280008A
CN107280008A CN201710535181.1A CN201710535181A CN107280008A CN 107280008 A CN107280008 A CN 107280008A CN 201710535181 A CN201710535181 A CN 201710535181A CN 107280008 A CN107280008 A CN 107280008A
Authority
CN
China
Prior art keywords
dietary supplements
folium artemisiae
artemisiae argyi
oleuropein
extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710535181.1A
Other languages
Chinese (zh)
Inventor
迈克尔·托马斯·麦克纳马拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sunshine Medical Operation Co Ltd
Original Assignee
Sunshine Medical Operation Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sunshine Medical Operation Co Ltd filed Critical Sunshine Medical Operation Co Ltd
Priority to CN201710535181.1A priority Critical patent/CN107280008A/en
Publication of CN107280008A publication Critical patent/CN107280008A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/4525—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with oxygen as a ring hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/282—Artemisia, e.g. wormwood or sagebrush
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention provides a kind of dietary supplements, including 60 80 parts of Folium Artemisiae Argyi extracts, 10 20 parts of oleuropeins and 10 20 parts of piperings.The dietary supplements of the present invention can further comprise upper dietetic product and/or pharmaceutically acceptable carrier and/or auxiliary material peroral dosage form is made.The preparation method of the dietary supplements of the present invention is to prepare Folium Artemisiae Argyi extract, oleuropein and pipering according to predetermined ratio；And mix Folium Artemisiae Argyi extract, oleuropein and pipering.The dietary supplements of the present invention has remarkable anti-oxidant and antiphlogistic effects, available for preparing health products and medicine.

Description

A kind of dietary supplements and its preparation method and application
Technical field
The invention belongs to medicines and health protection field, in particular it relates to a kind of dietary supplements and preparation method thereof and Using.
Background technology
Today, include ischemic heart disease, apoplexy, cancer, glycosuria to the most common reason of dead related disease Disease, hypertension, arthritis, obesity, chronic obstructive pulmonary disease (COPD) and other cigarettes and air pollution relevant disease, nerve are moved back Row disease such as Alzheimer's disease and Parkinson's disease.
Have confirmed, above-mentioned many diseases can be prevented.Such prevention can be based on several factors, wherein a lot It is relevant with heredity, diet, life style and environmental problem.
Inflammation.Today, the common factor and a series of pathology of referred to as inflammation of many most common and serious diseases Process is relevant.Chronic body inflammation, which is one, is used for the broad terms of a series of reaction of body chemistries and phenomenon, and it is to body Metabolic state and process have significant impact.
Inflammation is beneficial to human body.Body can produce a series of to the inflammation that sunburn, influenza virus or skin burn are caused The reaction of immunology and the circulatory system, to repair skin injury or influenza related tissue damage.Therefore under many circumstances, it is scorching Disease is beneficial to body.
However, inflammation and the generation and exacerbation of specified disease that chronic body illness is produced have very big relation.It is cardiovascular Disease, cancer, arterial hypertension, diabetes, metabolic syndrome, hypercholesterolemia, arthritis, obesity and Alzheimer Disease all has shown that relevant with the abnormal rise of chronic internal inflammation.Can be to specific biomarker, particularly C- reaction eggs In vain (CRP), measure to prove these inflammation.
Inflammation is also likely to be the direct result of intensive to calorie, natural deletions fiber excessive food consumption, this increase The risk of increased weight.Such increased weight can produce the accumulation of stomach fat and interior fat.Excessive stomach fat Increase with interior fat and the yield for the inflammatory molecule for being referred to as cell factor that can enter the circulatory system has very big Relation.So, they can cause the inflammatory response of body, so as to increase the risk of relevant disease.
A kind of most obvious inflammatory molecule that stomach fat is produced is interleukin-6 (IL-6).From consumption belly fat The vein of fat receives in the portal vein of blood and has measured high-caliber IL-6.In addition, high-caliber IL-6 and high blood water Flat CRP is directly related.
Disease caused by a kind of common and inflammation that is known is atherosclerosis.Circulate inflammatory cytokine chemical combination Thing may change the integrality of the crucial vascular endothelial cell of body, the coronary artery of such as heart and the arteria carotis of neck.With Inflammatory response afterwards promotes the abnormal accumulation of the fat cell of arterial endothelial cell.Such case is referred to as Atherosclerosis Change, if developing as one pleases can cause blood vessel diameter to significantly reduce to cause perfused tissue not enough.
Fat aggregation in vascular wall is referred to as patch.In some cases, especially in height inflammatory conditions or experience During oxidative stress, unstable patch may lose its integrality, cause plaque rupture.This phenomenon and the spot from damage Block is relevant and ultimately causes thrombosis, and wherein thrombus is clot.Such Arterial thrombosis is to cause most heart The reason for a variety of apoplexy.
Chronic body inflammation is relevant with elevated fasting blood glucose level, while also relevant with the increase of glycated hemoglobin.It is super Weight and obese individuals not coincidence, because excessive internal organ and stomach fat also can be to the works of the circulation insulin produced by pancreas With resistance is produced, the long-term rise of blood sugar level is ultimately resulted in.This is referred to as diabetes B.
Also easily increase suffers from the risk of malignant tumour to patient with chronic body inflammation.With other healthy individual body phases Than, some most common human cancers, including lung cancer, colorectal cancer, breast cancer, liver cancer, stomach cancer and prostate cancer, easily send out Life is with the increased chronic body inflammatory patients of level.
Researcher is it has been proved that individual body phase of the patient with Alzheimer's disease and Parkinson's disease with being not suffering from these diseases Than with higher levels of level of inflammation.
Oxidative stress.In human body, the physiology course referred to as aoxidized is vital for production energy.So And, during production energy, it can also produce free radical derived from oxygen.Sometimes the generation of such free radical is uncontrolled simultaneously The histocyte of the mankind may be damaged.
Under normal physiological conditions, atom has paired outer-shell electron, and therefore unobvious to adjacent tissue Reactivity.Free radical is defined as substantially increasing its tissue reactive containing not paired electronics.In biotic environment Under, due to these free radical atoms or compound may not make any distinction between with neighbouring molecule and cell effect, therefore from It is probably breakneck by this tissue reactive of base.
Free radical may produce referred to as " electronic theft " (electron stealing) phenomenon, and the phenomenon causes tissue Aoxidize and destination organization molecule may be made to lose activity.If the concentration of free radical is sufficiently large, these reactions are likely to result in Extensive cellular damage.
Oxidative stress simplest form is likely to be harmful tissue-damaging process, and the process is having excessive free free Base or lack anti-oxidizing compounds or this in two situation occur in the presence of simultaneously.Damaged as caused by free free radical activity is out of control Wound is referred to as oxidative stress.
The scope of this tissue damage can be conditioned or reduce, and be utilized depending on anti-oxidizing compounds Relative biological is neutralized Degree.These antioxidants are directly with free radical reaction and neutralizing their reactivity, linked groups' poison with low degree Property.
Oxidative stress may also result in the oxide-containing chemical material excess for being referred to as active oxygen (ROS).The drastically liter of ROS concentration It is high that there may be obvious cellular damage.
Oxidative stress may be produced by many reasons, including diet is bad, toxin, radiation, medicine and air pollution.The world The surrounding air in upper many areas contains a large amount of pollutants.It even itself may be exactly free to have some in these air pollutants Free radical, such as nitrogen dioxide.The result of these air pollutants of acute and Long Term Contact is that oxidative stress occurs for lung, once Pollutant is drawn through and into the circulatory system, and the oxidative stress that lung occurs can further result in systemic oxidative stress.
Lung's oxidative stress caused by air pollution can further trigger wider inflammatory reaction, then can in body It can discharge more free radicals.In the case where lacking enough polyphenoils, this tissue damage would is that significantly.
Especially, endonuclear DNA can be influenceed by being related to the free radical tissue damage of cell tissue nucleic acid.Therefore, for a long time Contact stain thing can trigger cell effect therewith, and some of which cell effect can trigger cancer.
Particulate air pollutant, especially gas-kinetic diameter are less than 10 μm of particle, are special for human health Dangerous, because they can cause significant oxidative stress and free radical to produce.
If developed as one pleases, this particulate air pollutant can also cause the damage of significant bodily tissue, local and complete The inflammation of body, and ultimately form cancer cell and trigger other inflammation related diseases.
In some areas, air pollution has become chief city human health problems.Particularly, air pollution particle Different sizes and composition they endanger human healths ability in especially their induced oxidations stress ability in play Remarkable effect.Ultrafine particle and other fine particulate materials less than 0.1 μm may be inhaled into and thin by the macrophage of body Born of the same parents and epithelial cell absorb.This absorption is likely to induce human body oxidative stress.
In addition, other air pollution compounds, such as polycyclic aromatic hydrocarbon (PAH), also materially increasing oxidation should Swash the risk with final tissue damage.
Anti-oxidant compounds, such as polyphenol and flavonoids, with the ability for removing these free radicals, it is meant that their energy Enough neutralize the harm that these free radicals are caused to human cell.In this case, these nutrient for plants, polyphenol and flavonoids Reduce the harm of cell that free radical causes and tissue damage.
The neutralization of free radical can bring a variety of benefits to human body.These benefits include reducing following disease or symptom Risk：Coronary heart disease, cardiovascular and cerebrovascular disease, cancer and other neoplastic syndromes, diabetes, obesity, nerve degenerative diseases, pass Section is scorching, pollution is related to smoking tissue damage and the tissue aging of acceleration.
Another elevated common reason of interior free yl concentration is muscular training, particularly the exercising with oxygen of high intensity. Increased Skeletal Muscle Contraction produces a large amount of excessive free radical compounds.
The generation of this free radicals a large amount of caused by motion, hinders ability to act of the muscle in optimum level, extends The recovery period of overexercise, and may improve the danger of muscle damage, it is referred to as pulled muscle or muscle tear.
The clinical research for performing high intensity interval tranining (HIIT) sportsman is shown, when sportsman takes oral anti-oxidant During agent, its energy production and athletic performance are optimal.It has been shown that HIIT its there is suppression to be referred to as the bones of human body " energy plants " The ability of bone Muscle Mitochondria, to eliminate the accumulation of the free radical related to moving.
The content of the invention
Technical problem solved by the invention is lacked in the prior art while having anti-oxidant and antiinflammatory action and in people The high dietary supplements of internal bioavailability.In order to solve the technical problem, the invention provides a kind of dietary supplements And its preparation method and application.
On the one hand, the invention provides a kind of dietary supplements, count by weight, the dietary supplements includes：60- 80 parts of Folium Artemisiae Argyi extracts, 10-20 parts of oleuropeins and 10-20 parts of piperings.
Foregoing dietary supplements, is counted by weight, and the dietary supplements includes：65-75 parts of Folium Artemisiae Argyi extracts, 12-18 parts of oleuropeins and 12-18 parts of piperings.
Foregoing dietary supplements, is counted by weight, and the dietary supplements includes：70 parts of Folium Artemisiae Argyi extracts, 15 parts Oleuropein and 15 parts of piperings.
Foregoing dietary supplements, the Folium Artemisiae Argyi extract extracts from the whole aerial part of folium artemisiae argyi plant.
Foregoing dietary supplements, the oleuropein extracts from olive fruits, olive water extract and/or olive oil.
Foregoing dietary supplements, the dietary supplements further comprises that dietetic product is upper and/or can pharmaceutically connect The carrier and/or auxiliary material received.
Foregoing dietary supplements, the dietary supplements is made into peroral dosage form；Preferably, the peroral dosage form is scattered Agent, tablet, capsule, granule, oral liquid or pill.
On the other hand, the invention provides the preparation method of foregoing dietary supplements, the preparation method includes：According to Predetermined ratio prepares Folium Artemisiae Argyi extract, oleuropein and pipering；And mix Folium Artemisiae Argyi extract, oleuropein and pipering Close.
Foregoing preparation method, the Folium Artemisiae Argyi extract uses supercritical CO2Abstraction technique and/or moisture film extraction filtering skill Art is prepared.
Foregoing preparation method, the oleuropein is prepared using moisture film extraction filtering technique.
Foregoing preparation method, the pipering uses supercritical CO2Abstraction technique is prepared.
On the other hand, health products or medicine with antioxidation are being prepared the invention provides foregoing dietary supplements Application in product.
On the other hand, health products or medicine with antiinflammatory action are being prepared the invention provides foregoing dietary supplements In application.
On the other hand, the invention provides foregoing dietary supplements prepare prevention and/or treat it is following any one Or the application in the health products or medicine of a variety of diseases or symptom：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery Hardening, cancer, Arterial Hypertention, diabetes, Health cost caused by hypercholesterolemia and similar relevant disease, cigarette, Oxidative stress and tissue damage, age-related body aging, overweight and fat, bacterium infection, nerve are moved back caused by pollution Loss and motion loss that row disease, skin symptom, ultraviolet trigger.
The beneficial effects of the invention are as follows：The present invention dietary supplements is anti-oxidant and antiphlogistic effects significantly, to it is a variety of with it is scorching Disease or the related disease of oxidative stress or symptom have good prevention or therapeutic action.The dietary supplements of the present invention, which has, matches somebody with somebody Fang Xinying, it is with low cost, evident in efficacy, the advantages of have no toxic side effect, and be applicable to industrialized production.
Embodiment
In order to be fully understood by the purpose of the present invention, feature and effect, by following embodiments, detailed is made to the present invention Describe in detail bright.The process of the present invention is in addition to the description below, and remaining is using the conventional method or device of this area.
Folium artemisiae argyi (Artemisia argyi)
Folium artemisiae argyi is, in the widely distributed herbaceos perennial of China, and to have been used to treatment infection, inflammatory disease, malaria Disease, diarrhoea, hepatitis, cancer [Adams M, Efferth T , ＆Bauer R.Artemisia argyi.Planta Medica, 2006；72：862-864.；Cai P.Lishizhen Medicine and Materia Medica Research, 2001；1： 1137-1139；Khan, et al.Evidence-Based Complementary and Alternative Medicine, 2012] and in the traditional Chinese medicine of dyshaemia.
Describe the plant chemical ingredient of this plant and numerous researchs of pharmacological property have shown that it contains many lifes Thing reactive compound.These bioactive compounds include cumarin, glycoside, flavonoids, polyacetylene, monoterpene, sterol, three Terpene, Sesquiterpene and essential oil.
The biological effect that some of these bioactive compounds have been confirmed includes：Anti-diabetic [Adams JD, Garcia C , ＆Garg G, Chinese Medicine, 2012；3：116-123], anti-oxidant [Ferreira JF, et Al.Molecules, 2010；15(5)：3135-3170], anti-proliferate, anti-inflammatory [Cai P.Lishizhen Medicine and Materia Medica Research, 2001；1：1137-1139], anticancer, antiallergy [Ji HY, et al, Molecules, 2010；15(9)：6466-6475], and antiulcer [Yoon KD, Chin YW, Yang, MH , ＆Kim J.Food Chemistry, 2011；129：679-683].
Have confirmed, flavone compound has anticancer and anti-proliferate characteristic.Especially, flavones known to two kinds has shown Referred to as SW 620 human cancer cell line's growth can be suppressed by showing.SW 620 is derived from human colon cancer cell.
Anticancer effect known to folium artemisiae argyi can suppress the growth of human colon cancer cell in two ways.They directly suppress knot Colon-cancer cell is bred, also, they also suppress angiogenesis, thus prevent the cancer life generally induced by colon cancer maintain and New vessels is formed.
Oleuropein (Oleuropein (OL))
Oleuropein (OL) is main bitter taste secoiridoid glycoside family phenolic compounds.Its derivative includes 2 kinds of masters The olive source alcohols wanted：1.3,4- dihydroxyphenyl ethanols, also referred to as oleuropein (HT), and 2. tyrosols, also referred to as Tyrosol (TY).
OL is present in the olive leaf without processing with the olive fruits before maturation, passes through chemistry after fruit maturation Method and enzyme process, which decompose OL, can produce HT and TY.
OL is hydrophilic compounds, therefore, the concentration in virgin oil (VOO) and Extra Virgin (EVOO) It is very low.
Oleuropein has a variety of health benefits having confirmed and medical application.For example：
It is anti-oxidant.OL contains positively charged oxygen on its phenol carbocyclic ring, and OL chemical constitution makes it have removing free radical Ability and can be as protecting barrier of the human body from tissue damage and aging.
Anti-aging.Effects of the ROS to tissue and cell can strengthen aging course.ROS and polyphenoils in human body Protective effect between unbalance can cause aging [Halliwell B：Oxidative stress and cancer：have We moved forward Biochem.J 2007,401：1-11.]；[Duracková Z：Some current insights Into oxidative stress.Physiol.Res.2010,59：459-469].Oleuropein is absorbed and neutralized by it The antioxidation delaying sanility process of ROS materials.
High biological is available.The antioxidant that target tissue and organ can not be reached with sufficient amount will not be brought to the mankind as The benefit that compound as OL can reach, it has already been proven that OL is high biological available [Cicerale S, Lucas LJ, Keast RS：Antimicrobial, antioxidant and anti-inflammatoryphenolic Activities in extra virgin olive oil.Curr.Opin.Biotechnol.2012,23：129-135.]； [Cicerale S, Lucas L, Keast R：Biological activities of phenolic Compoundspresent in virgin olive oil.Int.J Mol.Sci.2010,11：458-479.].
The main mechanism of OL effects includes：1) free radical scavenger and free radical chain fracture；2) resisting oxidation free radical；With 3) Metal-chelator.
OL catechol structure makes them remove peroxy radical and break peroxidating chain reaction to produce stabilization Resonant structure [Tripoli E, Giammanco M, Tabacchi G, DiMajo D, Giammanco S, La Guardia M： The phenolic compounds of olive oil：Structure, biological activity Andbeneficial effects on human health.Nutr Res Rev 2005,18：98-112.]；[Bulotta S, Oliverio M, Russo D, Procopio A：Biological Activity ofOleuropein and its Derivatives.In Natural Products.Edited by Ramawat KG, M é rillon JM.Berlin： Heidelberg Springer-Verlag；2013：3605-3638.].
Angiocardiopathy (CVD).OL antiphlogistic effects are known.CVD and chronic states body inflammation height correlation, Wherein body inflammation is caused by several factors, such as heredity, obesity, diabetes, smoking, exogenous pollution, diet, do not move and Other factorses.
OL can help to promote circulation by vasorelaxation action, reduce the influence of CVD results.It is anti-oxidant.Antiplatelet Aggregation.Thrombus in important blood vessels (such as the coronary artery and arteria carotis and cerebral artery of heart) causes poisonous and is typically to cause The cardiovascular event of life, such as miocardial infarction (heart disease) and apoplexy.OL resistant to aggregation effect helps to prevent such event..
Ldl cholesterol oxidation.LDL-C (LDL-C) is typically considered " bad " courage Sterol, because its internal arterial wall (endothelium) causes Atherosclerosis (blood platelet generation).However, working as LDL- C is after peroxidating, and their effect is significantly enhanced, and wherein LDL-C oxidation is adjusted in human body by copper sulphate abduction mechanism Section.Oleuropein slows down the circulatory diseases process by its scavenging action to HOCl, and HOCl is anti-in LDL-C lipid peroxidations Effect [Carr AC, Tijerina T, the Frei B of initiator is played during answering：Vitamin C protects against and reverses specifichypochlorous acid-and chloramine-dependent modifications Of low-density lipoprotein.Biochem.J 2000,346：491-499.].Thus, removings of the OL to HOCl is made With to lipoid peroxidization resistant and LDL-C oxidation can be reduced, the atherosclerosis disease of arterial endothelium is caused to deteriorate drop It is low, so as to reduce the risk of angiocardiopathy and cardiovascular event (such as miocardial infarction and apoplexy).
Pipering (Piperine)
Pipering (black pepper piper longum (piper longum) extract) is a kind of bioavilability reinforcing agent.Bioaugnentation Agent has the physiologic effect for improving the concentration that the nutraceutical applied is organized in target end organ.For example, once containing required chemical combination The material of thing is ingested, and bio-enhancer can improve the amount or target organ concentration of compound.The concentration of this raising has to health Profit, because bioactivity of the compound of intake in target end organ organizes is extended or strengthened.
A kind of main favorable property of pipering is that it can suppress the enzyme drug biotransformation reaction in liver.Pepper Alkali being capable of strong inhibition liver sausage aryl hydrocarbon-hydroxylase and UDP-glucoronosyl/transferase.The result of reduction liver and intestines degraded is Chinese mugwort The more multi cycle of leaf extract and OL, so as to bring more required positive health effects.
The Bioaugnentation effect of pipering is also attributed to the enhancing of intestinal absorption to a certain extent, and this is that pipering is acted on The result of the ultra microstructure of microvillus on intestinal brush border.Enhanced absorption improves concentration of the nutriment compound in serum, So that its bioavilability for receiving blood circulation and perfused tissue to tissue is bigger.
Although current research indicates folium artemisiae argyi and oleuropein has anti-inflammatory and antioxidant properties, pipering is biological profit Expenditure reinforcing agent, the present inventor by research it has unexpectedly been discovered that, when above-mentioned substance is according to special ratios carry out group Anti-inflammatory and antioxidant properties are significantly increased during conjunction.
Specifically, according to an aspect of the present invention, the invention provides a kind of dietary supplements, count by weight, The dietary supplements includes：60-80 parts of Folium Artemisiae Argyi extracts, 10-20 parts of oleuropeins and 10-20 parts of piperings；Preferably, originally The dietary supplements of invention includes：65-75 parts of Folium Artemisiae Argyi extracts, 12-18 parts of oleuropeins and 12-18 parts of piperings；More preferably Ground, dietary supplements of the invention includes：70 parts of Folium Artemisiae Argyi extracts, 15 parts of oleuropeins and 15 parts of piperings.
The present inventor by research it has unexpectedly been discovered that, when Folium Artemisiae Argyi extract, oleuropein and pipering are pressed When being combined according to ratio of the invention, cooperated between three, with synergy.
On the one hand, their own advantage is passed through into the prevention from suffering from the diseases of offer height on the basis of subassembly at these three And the anti-oxidation characteristics after combination, reduce or eliminate chronic body inflammation and oxidative stress, the internal free radical of reduction and activity The concentration of oxygen (ROS)；These three anti-oxidant and anti-inflammatory components act on the mankind and animal body specific to prevent by be combineding with each other Disease, this ability is further enhanced by pipering, and pipering can take in the intestines of nutrient for plants compound by increasing Road absorbs, and by reducing its internal metabolism elimination in liver and intestinal wall, and then increase the nutrient for plants of its intake The bioavilability of compound.
Particularly, in terms of pre- anti-cancer, such as colon cancer and many other alimentary tract cancers and other body parts The cancer of presence, dietary supplements of the invention is highly effective.Oleuropein is anticancer phenolic compound, it was verified that olive Bitter glycosides suppresses several mechanism of growth of cancer cells.Similar to folium artemisiae argyi, oleuropein is bred to cancer also has inhibition.The chemical combination Thing spreads to cancer cell also has direct inhibition.In the dietary supplements of the present invention, Folium Artemisiae Argyi extract and olive are bitter Glycosides combines the permanent anticancer effect obtained and further strengthened by pipering.Specifically, dietary supplements of the invention is not any Side effect, the combination of two kinds of naturally occurring phytochemicals can suppress the painstaking effort of growth of cancer cells and the induction of pre- anti-cancer Pipe is formed, so as to flow to the blood flow of cancer cell, these effects further can be strengthened by the Bioaugnentation effect of pipering, so that The high-efficient pure natural nutrient for plants replenishers of the pre- anti-cancer of the present invention are obtained.
On the other hand, when Folium Artemisiae Argyi extract, oleuropein and pipering are combined according to aforementioned proportion, by with olive Olive hardship glycosides and Folium Artemisiae Argyi extract bioactivity plant nutrient synergy, the positive health effect of pipering have also obtained pole Big raising and amplification.These effects include：Anti-inflammatory, anti-oxidant, anti-rotation shifting, antibacterial, anti-mutation and antitumor and anti-wither Die, these effects are the pipering other properties healthy and beneficial to humans and animals, and in oleuropein and Folium Artemisiae Argyi extract These effects of the lower pipering of effect have obtained enhancing and further activation.Except play bioperformance enhancing agent function it Outside, by suppressing and being quenched free free radical and reactive oxygen species, and by reducing peroxidatic reaction of lipid and positive shadow Intracellular mercaptan state, antioxidant molecule and antioxidase are rung, pipering is also prevented from oxidative stress associated tissue damage.
Folium Artemisiae Argyi extract, oleuropein and pipering are not combined according to the ratio of the present invention, three's synergy, and not The replenishers matched using the present invention are compared, and the anti-oxidant and antiphlogistic effects of dietary supplements of the invention are not only significantly carried Height, and effect is lasting.Compared with only taking any one or two kinds in Folium Artemisiae Argyi extract, oleuropein and pipering, or Person, compared with not using the replenishers that the present invention is matched, the antioxidant effect of dietary supplements of the invention is significantly higher than three kinds The independent effect sum of component.It is a kind of systematically measure and compare food and the method for the oxidation resistance of replenishers be measurement oxygen from By base absorbability (ORAC).The ORAC of oleuropein is 12,000 TE/ grams of micromole, and the ORAC of Folium Artemisiae Argyi extract is 57-112 TE/ grams of micromole, the ORAC of pipering is very small and is comparatively speaking insignificant, therefore the ORAC of oleuropein compares folium artemisiae argyi Extract is big 100 times.And for the dietary supplements of the present invention, its effect effect sum more independent than each component is big at least 50-100%.
The main component of Folium Artemisiae Argyi extract is anti-oxidant polyphenol, flavonoids and carotenoid compounds, and cumarin, Glucosides, polyacetylene, monoterpene, sterol, triterpene, sesquiterpene, eudesmane, polyunsaturated fatty acid, vitamin C, essential amino acid And essential oil.
Folium Artemisiae Argyi extract can extract from the whole aerial part of folium artemisiae argyi plant, including leaf and stalk.Preferably, use Supercritical CO2Abstraction technique and/or moisture film extract filtering technique to obtain Folium Artemisiae Argyi extract.Both extractive techniques avoid using Ethanol, methanol or other any chemical reagent.Moisture film extraction filtering technique only uses water as solvent, and supercritical CO2Extraction Technology is taken (for example, using high pressure (100-150 bars) and the supercritical CO of low temperature (40 degrees Celsius)2Abstraction technique) it is in high-pressure section Lower of part uses the carbon dioxide being perfectly safe.Moisture film extracts filtering technique while avoiding using chemical solvent, makes folium artemisiae argyi Extract has reached that total phenolic compound (polyphenol) and flavonoids (other bioactivity beneficial compounds) have highest concentration. The antioxidation activity for the Folium Artemisiae Argyi extract being obtained by extraction by water is than other solvents such as methanol, acetone, ethanol and chloroform extraction The activity gone out is high.Supercritical CO is preferred in order to obtain the extract in optimal anti-inflammatory bioactivity, folium artemisiae argyi flower2Extract Technique productions.Therefore, the present invention extracts the extract and supercritical CO of filtering technique using moisture film2The extract institute of abstraction technique The composition of composition is come the phenols of realizing highest level is anti-oxidant and anti-inflammatory bioactivity.
In a kind of preferred embodiment, folium artemisiae argyi extraction process includes following workshop section：
1. the plant material collection stage
Before the extraction stage, first the folium artemisiae argyi plant material of harvest is screened, is cleaned, sterilized and ground, to give birth to Produce the biological active component of final products.
The stalk of the leaf of plant and aerial part is transported into extraction factory first and carries out cleaning and plant part sorting. In order that the yield of anti-oxidant phenolic resin, flavonoids, carotenoid Phytochemistry compound is maximum, it is necessary to make whole Plant material selection and processing process system.This will need following device：
(1) it is used for the conveyer belt and system for collecting plant material
(2) disleave and vibratory sieve separation device, to screen a variety of plant positions
(3) washing and sorting line, for cleaning and screening vegetable matter
2. the plant material primary treatment stage
Folium artemisiae argyi plant material must mechanically and chemically be handled to maximize the extraction of bioactive compound.
Mainly extracted to avoid using organic solvent using distilled water.Use isolation technics, particularly film skill Art, to carry out flooding and purifying process.These technologies include ultrafiltration, filter, dialysis and electrodialysis successively.
Water soluble active component is extracted in hot water of the temperature less than 80 DEG C, such as anti-oxidant phenolic aldehyde, flavonoids and class are recklessly Radish extract plant chemical compound.Using supercritical CO2Abstraction technique extracts other hydrophobic substances, such as saturation and insatiable hunger The aliphatic acid of sum.
The bioactivity vegetalization material extracted from folium artemisiae argyi has variable hydrophobicity and a partial hydrophilicity, therefore can be with Using supercritical CO2Abstraction technique and water film filtering technology extract these materials.Characterization of The Products is extracted come closely using analysis Monitoring optimization is extracted.The standard of Such analysis includes：Uniformity, heat endurance, humidity, fragrance, freezing point, electric conductivity, pH and Gelling ability.
Carefully the formulation of concern final products is to produce various pulvis, tablet, capsule and liquid extract, and this is very Important.
In a word, the folium artemisiae argyi extraction equipment needed for above-mentioned technique is as follows：
(1) plant grinding device, including industrial grinding mills and plant and seed shredding machine.
(2) supercritical fluid CO2Extraction equipment, is extracted for hydrophobic plant chemical compound.
(3) solvent extraction system.This is directed to use with extracting for water-soluble substances or the water or ethanol of non-water soluble substance Device.
(4) the thick system for carrying, collecting and storing for folium artemisiae argyi, by stainless cylinder of steel and copper pipe of reservoir group into.
(5) system in extraction process needed for bulk liquids transhipment, is made up of pump and pipeline.
3. separation and enriching stage
Crude extract (including water and alcoholic solution) must be refined by using the isolation technics of filtering and Distallation systm. Then, obtained fraction is further concentrated by evaporating, to obtain the bioactive substance of higher concentration.
Further improve the concentration of purified extract using membrane technology, the membrane technology is nanofiltration, counter-infiltration, it is dense to freeze Contracting, vacuum evaporation.
The equipment in these stages includes：Membrane filtration system, stainless cylinder of steel and reservoir system for collecting liquid in the middle of liquid, Pump and pipe-line system for transporting liquid, concentration and drainage equipment：Vacuum, fluid bed and thin film evaporator.
4. refining stage
Then, the liquid fraction of the concentration obtained to separation and concentration process further carries out refining operation, to incite somebody to action Specific compound (polyphenol and flavonoids) is separated with other materials (salt, sugar and protein).This effectively increases bioactivity The yield and purity of compound.Further, it is necessary to convert liquids to stable formulation such as powder, and therefore need to dry system System.
Equipment：
(1) chromatographic system, it is equipped with the industrial columns of ion-exchange chromatography
(2) drying system：Fluidized bed drying system, spray drying system, freeze-drier
The present invention dietary supplements in, the oleuropein extract from olive fruits, olive leaf, olive plant chimney stalk, Olive water extract and/or olive oil, it is preferred to use moisture film technology (i.e. moisture film extraction filtering technique or water film filtering technology).Use Water is used as unique solvent, it is to avoid use chemical substance, and the antioxidation activity of maximum can be realized by water film filtering technology.Film mistake Filter technology is including the use of ultrafiltration, filter, dialysis and electrodialysis.The concentration of purified extract can in the steps below be entered using membrane technology OK：Nanofiltration, counter-infiltration, freeze concentration and vacuum evaporation.
There are many methods can be for extracting pipering from black pepper.It should be noted that start extraction process it Before, it is necessary to first black pepper is ground using industrial grinding mills.
Pipering can use dichloromethane to extract.Using aqueous cosolvent, it can bring high yield and high selectivity. Another conventional method be using potassium hydroxide solution handle the solvent-free residue from black pepper ethanol extract so as to Remove the insoluble residue washed in resin and warm ethanol.Alkaloid is crystallized during this by cooling down.This is directed to use with Water and ethanol extractor for water-soluble substances.
Discounting for cost issues, supercritical CO is preferably used under elevated pressure conditions2Abstraction technique come extract recklessly Green pepper alkali, because this method is only using harmless and overall safety carbon dioxide and to bring high-purity, high yield under high pressure The pipering of amount.
In addition to the above-mentioned content referred to, the other specific process parameters and condition being related in the above method are this area skills Art personnel can determine that the appropriate adjustment of process conditions will not be to extract in meals of the invention according to needs of production Effect played in replenishers produces materially affect.
The dietary supplements of the present invention can adopt the Folium Artemisiae Argyi extract, oleuropein and pipering of extraction according to aforementioned proportion Formed with conventional method is well mixed.
The dietary supplements of the present invention can further comprise that dietetic product is upper and/or pharmaceutically acceptable carrier with/ Or auxiliary material, for example, filler, disintegrant, lubricant, suspending agent, adhesive, sweetener, flavouring, preservative etc..Filler Including：Starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose etc..Disintegrant includes：Carboxymethyl forms sediment Powder sodium, PVPP, low-substituted hydroxypropyl cellulose, Ac-Di-Sol etc..Lubricant includes：It is stearic Sour magnesium, lauryl sodium sulfate, talcum powder, silica etc..Suspending agent includes：Polyvinylpyrrolidone, microcrystalline cellulose, sugarcane Sugar, agar, hydroxypropyl methyl cellulose etc., adhesive includes, starch slurry, polyvinylpyrrolidone, hydroxypropyl methyl cellulose Deng.Sweetener includes：Saccharin sodium, aspartame, sucrose, honey element, enoxolone etc..Flavouring includes：Sweetener and various Essence, preservative includes：Parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, chlorhexidine acetate, Eucalyptus oil etc..By adding corresponding carrier and/or auxiliary material, oral agents can be further made in dietary supplements of the invention Type, for example, powder, tablet, capsule, granule, oral liquid or pill etc..In actual production, those skilled in the art can The consumption of carrier and auxiliary material is determined according to actual needs, to reach optimal using effect.
When preparing final dietary supplement product, it is necessary to which the production system of such as tablet and capsule, what is be related to is specific Equipment is to prepare the powder compressibility needed for capsule and tablet.In addition, when preparing final dietary supplement product, also relating to And packing stage, i.e., final products are packed with solid dosage forms or liquid dosage form, be primarily related to be used for encapsulating products Blister packaging equipment and bottling line for fluid product.
Take in the restriction of application
According to another aspect of the present invention, it is turned into the invention provides foregoing dietary supplements in preparation with antioxygen Application in health products or medicine.
According to another aspect of the present invention, prepared the invention provides foregoing dietary supplements with antiinflammatory action Health products or medicine in application.
According to another aspect of the present invention, preparing prevention the invention provides foregoing dietary supplements and/or controlling Treat the application in the health products or medicine of any one or more following disease：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery sclerosis, cancer, Arterial Hypertention, diabetes, caused by hypercholesterolemia and similar relevant disease, cigarette Oxidative stress and tissue damage, age-related body aging, overweight and fat, bacterium sense caused by Health cost, pollution Loss and motion loss that dye, nerve degenerative diseases, skin symptom, ultraviolet trigger.
Specifically, dietary supplements of the invention has significant prevention and/or therapeutic effect to following disease or symptom：
1. angiocardiopathy, for example, apoplexy, myocardial infarction and artery sclerosis.The dietary supplements of the present invention is by reducing Inflammation reduces the risk of angiocardiopathy with anti-oxidant feature is improved, and can reduce the ability of blood formation thrombus grumeleuse, So as to reduce the ability of their blocked coronary arteries and arteria carotis inner chamber.
2. cancer, such as breast cancer, colon cancer, prostate cancer, cancer of pancreas, lung cancer, and some other tumours.This hair Bright dietary supplements can suppress growth of cancer cells or directly kill cancer cell, and effectively suppress cancer blood circulation.In addition, this The chemical signal of generation blood vessel of the dietary supplements of invention to being sent by cancer cell, which has, directly suppresses anti-angiogenesis work With so that causing the survival rate of the cancer cell contacted with dietary supplements of the invention reduces.
The research of inventor also has confirmed another mechanism of pre- anti-cancer, and it is based on passing through CNR1 promoters site The change of DNA methylation carrys out transient state up-regulation CNR1 tumor suppressor genes.
3. diabetes.The research of inventor shows, periodically takes the diabetic of the dietary supplements of the present invention, display Its fasting blood glucose level and the reduction of glycated hemoglobin (HbA1c) level.These results show the dietary supplements of the present invention to sugar Urine patient has positive effect.
Wherein, HbA1c is the subgroup for circulating red blood cell (red blood cell), and in diabetes and pre-diabetic, it contains Amount is higher than normal range (NR) (4-5.6%), and this is attributed to the irreversible glycosylation in corpuscular hemoglobin β chain N- ends.Scope is 5.7-6.4% HbA1c high value is consistent with pre-diabetes condition, so as to add the risk for suffering from diabetes.6.5% or Higher level is consistent with the diagnosis to diabetes.The dietary supplements of the present invention can reduce diabetes and prediabetes is suffered from The HbA1c levels of person.
4. cholesterol, particularly serum cholesterol.The dietary supplements of the present invention is to reduction serum low-density LP (LDL-C) has remarkable effect.
In addition, the dietary supplements of the present invention can cause the aobvious of the serum-concentration of OxLDL ELISA (ox-LDL) Writing reduction and the referred to as inflammation blood plasma biomarker of plasma C-reactive protein (CRP) significantly reduces.LDL-C pairs of oxidation The harm of cardiovascular health is bigger, because the LDL-C of oxidation produces artery, (particularly heart and arteria carotis is coronal dynamic Arteries and veins) fat lesion atherosclerotic plaque ability it is stronger.
In addition, the present invention dietary supplements can increase the serum levels of HDL (HDL cholesterol), this It is beneficial in terms of reducing the health risk for suffering from artery sclerosis.The research of inventor also shows that the dietary supplements of the present invention is improved HDL cholesterol efflux abilities, this can improve the healthy curve of user by improving the physiological function of body, so that clearly Manage the artery influenceed by atherosclerosis.
5. hypertension.Research in laboratory scope shows that dietary supplements of the invention significantly reduces systolic pressure And diastolic pressure.
Inventor also found that dietary supplements of the invention is significant to reduce blood pressure and improve with slight high by research The endothelial function of the young woman of blood pressure.Due to, it is known that the lifting in treatment hyperpietic of the function of arterial wall endothelial cell Act on, therefore endothelial function is extremely important in the elevated patient of blood pressure.Inner skin cell function is better, and blood pressure is more normal.
6. extend the life-span.The zoopery that inventor is carried out shows, the dietary supplements of the present invention is periodically taken in daily The life-span of the control group adult mice of dietary supplements of the life-span of adult mice than not taking in the present invention is extended.Herein In the positive health effect expection enumerated people can be produced similar prolong long-life effect.
The research of inventor is it is also shown that the dietary supplements of the present invention can slow down cell ageing by some physiological phenomenons Process.The present invention dietary supplements can energetically resist epigenetics (such as DNA methylation) change, telomere cut down, Protein homeostasis is lost and nutrition senses imbalance.The present invention dietary supplements can also prevent mitochondria dysfunction and Stem cell is exhausted and cell ageing.The dietary supplements of the present invention can prevent the oxidative damage to inhereditary material, so as to promote Enter genome stable.
7. smoking.It is well known that one of harmful mechanism of harmfulness of smoking health is drawn with main and secondary smoking The body inflammation-related risen.This inflammation causes various disease-angiocardiopathies and cancer to be most common two kinds.
Daily ingestion of dietary supplements of the invention helps to protect human body from harm of smoking.Its mechanism of action is： 1) prevents oxidative stress caused by smoke from cigarette, 2) prevents caused by nicotine the damage of lung's antioxidase and increased lipid Peroxidation, 3) prevents the inflammation damnification that is directly contacted between smoke from cigarette and bronchial wall tissue, 4) prevents nicotine Angiogenesispromoting effect, suppress the growth of tumour and cancer.
8. air pollution.Similar to smoke from cigarette, air pollution can cause oxidative stress, reduce body antioxidase, and And be proinflammatory disease.The dietary supplements of the present invention can protect the body from free radical and aoxidize to answer because of its anti-oxidation characteristics Swash.This protection is then also possible to prevent the sequelae of oxidative stress and air pollution, such as COPD and cancer.
9. skin protection.It is harmful to skin health exposed to ultraviolet (UV) radiation.Ultraviolet can be with the bottom of injured skin Layer scaffolding protein (being referred to as collagen and elastin laminin) suppresses skin corium fibroblast metabolism and produces collagen and bullet Property albumen, and produce also cause dermal tissue insult free radical.
The dietary supplements of the present invention helps to protect skin by eliminating skin free radical, so as to limit the group of correlation Knit damage.In addition, the dietary supplements of the present invention also enhances the generation of glutathione, this also contributes to protect the skin from certainly By the injury of base.
The dietary supplements of the present invention has direct inhibitory action to cancer cell, so as to contribute to the skin of protection exposure The cutaneum carcinoma induced from UV, the invasion and attack of such as melanoma.
10. arthritis.Arthritis be it is a kind of influence body joints inflammatory disease, including backbone, buttocks, knee, shoulder, Ancon, ankle, wrist, pin and pin.Soft tissue inflammation and cartilage inflammation of the dietary supplements of the present invention to arthritis and correlation With direct effect.
11. body weight.The present inventor is by studying discovery, the increasing that dietary supplements of the invention can lose weight Plus, so as to effectively prevent and treat obesity.
12. skin whitening.The dietary supplements of the present invention can directly reduce melanocyte activity and increase gluathione The generation in vivo of peptide, so as to be conducive to skin whitening.
13. mental health.The dietary supplements of the present invention is by promoting elimination intracerebral amyloid-beta to provide god Through protection, so that the prevention and treatment for nerve degenerative diseases such as alzheimer disease (AD) etc..
14. infection.Experiment in vitro proves that dietary supplements of the invention has the property of antibacterial, therefore the meals of the present invention Eating replenishers can be as the antiseptic applied to prevention enteron aisle and respiratory tract infection.
15. gene clock (Gene Clock).According to the research of inventor, clock Physiological effect base is had been reported that now Because (clock circadian regulator gene) CLOCK CpGs 1 and 8 ratios methylated are shown and single unsaturation Aliphatic acid (MUFA) [Milagro, F.I.et al.Clock, PER2and relevant with the intake of polyunsaturated fatty acid (PUFA) BMAL1 DNA methylation：Association with obesity and metabolic syndrome Characteristics and monounsaturated fat intake.Chronobiol.Int.2012,29,1180- 1194.].Once such material containing MUFA is Extra Virgin, it is also enriched in aldehydes matter, such as oleuropein, So oleuropein component of the invention has good effect for up-regulation Clock genes and BMAL1 gene activities.CLOCK bases Because (clock gene) is responsible for the biological clock rhythm system of body, 24 circadian rhythm 24s of the systematic direction gene expression in itself. The gene is relevant with fat and metabolic syndrome.Researcher has been found that patient and trouble with visceratonia abdominal fat obesity The patient for having metabolic syndrome shows the symptom (timer interrupt) of circadian rhythm disorders, and the illness can produce larger body weight Increase and then fat tendency, further development can cause diabetes and atherosclerosis [G ó mez-Santos, C.et Al.Circadian rhythm of clock genes in human adipose explants.Obesity 2009,17, 1481-1485].The dietary supplements of the present invention can regulate and control CLOCK gene activation approach, so as to help to prevent and reduction is made Factor in terms of the fat gene with metabolic syndrome.
Causality [Yoshino J, Klein S is also likely to be present between the suppression of CLOCK genes and diabetes B (Jul 2013).″A Novel Link Between Circadian Clocks and Adipose Tissue Energy Metabolism″.Diabetes.62(7)：2175-7].Therefore, the regulation and control to CLOCK genes help to prevent or reduce by 2 types The seriousness of diabetes.
The research of inventor displays that the epigenetic variation of CLOCK genes is relevant with breast cancer incidence raising, and this is Because the reduction that methylates of the CLOCK promoter regions of the women suffered from breast cancer.The dietary supplements of the present invention being capable of actively impact These are mutated and reduce their influences to CLOCK genes, so as to reduce the risk suffered from breast cancer.
The research of inventor is displayed that, causes have height between the somatic mutation of colorectal cancer and CLOCK mutation Correlation.The dietary supplements of the present invention helps to stablize CLOCK genes to prevent CLOCK to be mutated, so as to contribute to pre- CLOCK is mutated in the anti-microsatellite instability target gene for causing colorectal cancer.
Effect of the dietary supplements of the present invention on CLOCK gene expressions can also influence circadian rhythm period, thus regulation and control Sleep pattern.CLOCK and CYCLE albumen (circulating protein) (dBMAL genes) can form dimer, the compound finally with Period (per, Per1 gene) and timeless (tim genes) promoter combine to raise (stimulation) per genes and tim Gene.Therefore, dietary supplements of the invention is the adjusting control agent of CLOCK genes.The regulation activity can treat and prevent sleep Rhythm disorder, and the associated disorders caused by being travelled in multiple time zones, the i.e. time difference can be treated and prevented.
Embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to described reality Apply among a scope.The experimental method of unreceipted actual conditions in the following example, conventionally and condition, or according to business Product specification is selected.
The Folium Artemisiae Argyi extract used in following embodiments is divided into raw material using overcritical with the whole overground part of folium artemisiae argyi plant CO2Abstraction technique is obtained.Oleuropein is obtained by raw material of olive fruits using moisture film technology.Pipering is using black pepper as raw material Using supercritical CO2Abstraction technique is obtained.Wherein, supercritical CO2Condition in abstraction technique and moisture film technology is normal condition.
Embodiment 1
The composition of the dietary supplements of embodiment 1 is Folium Artemisiae Argyi extract 70mg, oleuropein 15mg and pipering 15mg.
Preparation method：
Folium Artemisiae Argyi extract 70mg, oleuropein 15mg and pipering 15mg are well mixed, the meals for producing embodiment 1 are mended Fill agent.
Embodiment 2
The composition of the dietary supplements of embodiment 2 is Folium Artemisiae Argyi extract 60mg, oleuropein 20mg and pipering 20mg.
Preparation method：
Folium Artemisiae Argyi extract 60mg, oleuropein 20mg and pipering 20mg are well mixed, the meals for producing embodiment 2 are mended Fill agent.
Embodiment 3
The composition of the dietary supplements of embodiment 3 is Folium Artemisiae Argyi extract 80mg, oleuropein 10mg and pipering 10mg.
Preparation method：
Folium Artemisiae Argyi extract 80mg, oleuropein 10mg and pipering 10mg are well mixed, the meals for producing embodiment 3 are mended Fill agent.
Embodiment 4
The composition of the dietary supplements of embodiment 4 is Folium Artemisiae Argyi extract 65mg, oleuropein 18mg and pipering 17mg.
Preparation method：
Folium Artemisiae Argyi extract 65mg, oleuropein 18mg and pipering 17mg are well mixed, the meals for producing embodiment 4 are mended Fill agent.
Embodiment 5
The composition of the dietary supplements of embodiment 5 is Folium Artemisiae Argyi extract 75mg, oleuropein 12mg and pipering 13mg.
Preparation method：
Folium Artemisiae Argyi extract 75mg, oleuropein 12mg and pipering 13mg are well mixed, the meals for producing embodiment 5 are mended Fill agent.
Embodiment 6
The composition of the dietary supplements of embodiment 6 is Folium Artemisiae Argyi extract 65mg, oleuropein 17mg and pipering 18mg.
Preparation method：
Folium Artemisiae Argyi extract 65mg, oleuropein 17mg and pipering 18mg are well mixed, the meals for producing embodiment 6 are mended Fill agent.
Embodiment 7：Oral liquid
The dietary supplement product of embodiment 7 is oral liquid, and the capacity of every oral liquid is 15ml, including：Folium artemisiae argyi is extracted Thing 70mg, oleuropein 15mg and pipering 15mg, sucrose 20mg, and water 15ml.
Preparation method：By Folium Artemisiae Argyi extract 70mg, oleuropein 15mg and pipering 15mg, sucrose 20mg is dissolved in 15ml water In, it is well mixed；Homogenization is then carried out in homogenizer, various nutritional ingredients is homogenized, obtains the stability of product To further guarantee；Then carry out filling, produce the oral liquid of embodiment 7.
Embodiment 8：Granule
The dietary supplement product of embodiment 8 is granule, including：Folium Artemisiae Argyi extract 70mg, oleuropein 15mg, 15mg Pipering, sucrose 10mg and starch slurry 20mg.
Preparation method：After all materials are mixed 4 minutes, the water of material gross weight 3% is uniformly poured into, is stirred 1 minute； Then, granulated using 1 millimeter of screen cloth rotary squeezing of diameter；Then, 105 DEG C of dryings 4 minutes；Finally, 20 DEG C cool down 1 point, and cross 2 Mm sieve, is produced.
Above-described embodiment is the present invention preferably embodiment, but present aspect embodiment not by above-described embodiment Limitation, other any Spirit Essences without departing from the present invention and the replacement made under principle, modification, combine, change, simplification Deng should be equivalent substitute mode, be included within protection scope of the present invention.Protection scope of the present invention should be with right What claim was limited is defined.

Claims (14)

1. a kind of dietary supplements, it is characterised in that count by weight, the dietary supplements includes：60-80 portions of folium artemisiae argyis are carried Take thing, 10-20 part oleuropein and 10-20 parts of piperings.
2. dietary supplements according to claim 1, it is characterised in that count by weight, the dietary supplements bag Include：65-75 parts of Folium Artemisiae Argyi extracts, 12-18 parts of oleuropeins and 12-18 parts of piperings.
3. dietary supplements according to claim 1 or 2, it is characterised in that count by weight, the dietary supplements Including：70 parts of Folium Artemisiae Argyi extracts, 15 parts of oleuropeins and 15 parts of piperings.
4. the dietary supplements according to claim any one of 1-3, it is characterised in that the Folium Artemisiae Argyi extract extracts from Chinese mugwort The whole aerial part of leaf plant.
5. the dietary supplements according to claim any one of 1-4, it is characterised in that the oleuropein extracts from olive Fruit, olive water extract and/or olive oil.
6. the dietary supplements according to claim any one of 1-5, it is characterised in that the dietary supplements is further wrapped Include in dietetic product and/or pharmaceutically acceptable carrier and/or auxiliary material.
7. the dietary supplements according to claim any one of 1-6, it is characterised in that the dietary supplements is made into mouth Oral dosage form；Preferably, the peroral dosage form is powder, tablet, capsule, granule, oral liquid or pill.
8. the preparation method of the dietary supplements described in claim any one of 1-7, it is characterised in that the preparation method bag Include：Prepare Folium Artemisiae Argyi extract, oleuropein and pipering according to predetermined ratio；And by Folium Artemisiae Argyi extract, oleuropein and Hu Green pepper alkali is mixed.
9. preparation method according to claim 8, it is characterised in that the Folium Artemisiae Argyi extract uses supercritical CO2Extract skill Art and/or moisture film extraction filtering technique are prepared.
10. preparation method according to claim 8 or claim 9, it is characterised in that the oleuropein extracts filtering using moisture film Technology is prepared.
11. the preparation method according to claim any one of 8-10, it is characterised in that the pipering uses supercritical CO2 Abstraction technique is prepared.
12. the dietary supplements described in claim any one of 1-7 is in the health products or medicine with antioxidation are prepared Application.
13. the dietary supplements described in claim any one of 1-7 is in the health products or medicine with antiinflammatory action are prepared Using.
14. dietary supplements described in claim any one of 1-7 prepare prevention and/or treat it is following any one or more Application in the health products or medicine of disease or symptom：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery sclerosis, Cancer, Arterial Hypertention, diabetes, Health cost, pollution caused by hypercholesterolemia and similar relevant disease, cigarette are drawn The oxidative stress and tissue damage, age-related body aging, overweight and fat, bacterium infection, nervus retrogression disease risen Loss and motion loss that disease, skin symptom, ultraviolet trigger.
CN201710535181.1A 2017-07-03 2017-07-03 A kind of dietary supplements and its preparation method and application Pending CN107280008A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710535181.1A CN107280008A (en) 2017-07-03 2017-07-03 A kind of dietary supplements and its preparation method and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710535181.1A CN107280008A (en) 2017-07-03 2017-07-03 A kind of dietary supplements and its preparation method and application

Publications (1)

Publication Number Publication Date
CN107280008A true CN107280008A (en) 2017-10-24

Family

ID=60099218

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710535181.1A Pending CN107280008A (en) 2017-07-03 2017-07-03 A kind of dietary supplements and its preparation method and application

Country Status (1)

Country Link
CN (1) CN107280008A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103655731A (en) * 2013-12-11 2014-03-26 张宗升 Natural pharmaceutical composition
US20140227198A1 (en) * 2013-02-13 2014-08-14 GRADS Holdings LLLP Shelf-stable olive extract-containing compositions and methods of use thereof
CN104623553A (en) * 2015-02-12 2015-05-20 王彦 Traditional Chinese medicine composition for treating annexitis
CN104800597A (en) * 2015-05-23 2015-07-29 周末 Traditional Chinese medicine composition for treating sphagitis
CN106174205A (en) * 2016-07-26 2016-12-07 厦门益力康生物科技有限公司 A kind of fruit ferment and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140227198A1 (en) * 2013-02-13 2014-08-14 GRADS Holdings LLLP Shelf-stable olive extract-containing compositions and methods of use thereof
CN103655731A (en) * 2013-12-11 2014-03-26 张宗升 Natural pharmaceutical composition
CN104623553A (en) * 2015-02-12 2015-05-20 王彦 Traditional Chinese medicine composition for treating annexitis
CN104800597A (en) * 2015-05-23 2015-07-29 周末 Traditional Chinese medicine composition for treating sphagitis
CN106174205A (en) * 2016-07-26 2016-12-07 厦门益力康生物科技有限公司 A kind of fruit ferment and preparation method thereof

Similar Documents

Publication Publication Date Title
CN101674821B (en) Composition for treating diabetes and metabolic disorders with quercetin, myrcetin and chlorogenic acid
CA2520207C (en) Composition comprising bamboo extract and the compounds isolated therefrom showing treating and preventing activity for inflammatory and blood circulation disease
CN102872306B (en) Compound selenium Chinese medicinal composition for treating cardiovascular and cerebrovascular diseases and preparation method thereof
JP2009126853A (en) Therapeutic agent
JP4686173B2 (en) Processed acerola containing polyphenol and / or vitamin C
CN101766274B (en) Antioxidant functional food composition containing bamboo-leaves flavones
KR20120003693A (en) Anti-obesity composition comprising red grape extracts, green tea extracts, soybean extracts, and l-carnitine
KR100976241B1 (en) Extract of sedum sarmentosum for alcohol oxidation and relieves hangover
WO2005087246A1 (en) Antitumor agent
WO2006090206A1 (en) Improved extracts of psidium guajava l., methods for its obtaining and use for the treatment of gastrointestinal disorders
CN107456505A (en) A kind of dietary supplements and its preparation method and application
KR101821925B1 (en) A composition comprising the complex extract of Poncirus trifoliata and Cinnamommum cassia for treating or preventing allergy desmatitis
JP2018506524A (en) Composition for prevention, amelioration or treatment of burnout syndrome
KR20120096764A (en) Functional food composition for the oral purpose with anti-inflammatory activity and it's method of manufacturing
JP2006016330A (en) Fat-burning accelerating agent
CN107280008A (en) A kind of dietary supplements and its preparation method and application
Hrelia et al. Quercetin and its metabolites in heart health
KR102185919B1 (en) Composition contaning herbal mixture extract for improving, alleviating or treating side-effect of anticancer drug
KR101738206B1 (en) A composition comprising the complex extract of Poncirus trifoliata and Cinnamommum cassia for treating or preventing allergy desmatitis
KR101934810B1 (en) Preparation for improving blood circulation comprising oriental medicinal extracts
CN107456504A (en) A kind of dietary supplements and its preparation method and application
CN107441109A (en) A kind of dietary supplements and its preparation method and application
KR101119647B1 (en) Torilis japonica extracts compositions for treating or preventing inflammatory diseases
KR101131719B1 (en) Pulsatilla koreana extracts compositions for treating or preventing inflammatory diseases
CN107518399A (en) A kind of dietary supplements and its preparation method and application

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20171024

WD01 Invention patent application deemed withdrawn after publication