KR101934810B1 - Preparation for improving blood circulation comprising oriental medicinal extracts - Google Patents

Abstract

The present invention relates to a preparation for improving blood circulation containing herbal medicinal extracts and, more specifically, to a preparation for improving blood circulation which uses various herbal medicine ingredients to efficiently extract each ingredient and mix the ingredients in an appropriate mixing ratio in consideration of useful functionalities and side effects of each ingredient, and uses a composition optimized in consideration of the taste of a product to be produced in the form of pills, tablets, capsules, injections, pouches, drinks or beverages. According to the present invention, the preparation for improving blood circulation can be useful in the prevention and treatment of diseases including hyperlipidemia, atherosclerosis, cerebral thrombosis and angina pectoris, has functionalities and activities that enhance the blood stream, ameliorate the angiosclerosis, improve the vascular endothelial function, alleviate stroke, dementia, palpitations, convulsions and pain, and facilitate the production and circulation of clear blood to the peripheral blood vessels, and thus can be appropriately used to patients who require such functionalities and activities.

Description

A preparation for improving blood circulation comprising oriental medicinal extracts containing herbal medicine extracts,

The present invention relates to an agent for improving blood circulation comprising herbal medicine extracts. More particularly, the present invention relates to an agent for improving blood circulation comprising various herbal medicine ingredients, Tablets, capsules, injections, pouches, drinks, or beverages to improve blood circulation. The present invention also relates to a composition for improving blood circulation in the form of pills, pills, drinks or drinks.

Thrombosis-related diseases, which account for about 39% of the world's deaths, are increasing in Korea due to westernization of diet, excessive stress, and aging population.

The global antithrombotic and anticoagulant market is expected to reach $ 24.4 billion by 2015, and sales should gradually increase until 2022. In particular, the incidence, prevalence, and number of diagnoses in developing countries such as China and India are expected to contribute to sales growth. The introduction of new oral anticoagulants, which are superior in terms of safety and efficacy, and pharmaceuticals and medicines with drug delivery methods that facilitate easy prescription and compliance are expected to replace existing treatments.

Streptokinase, urokinase, and tissue plasminogen activator (t-PA) are known to be currently used as thrombolytic enzymes, and mainly plasminogen in vivo is called plasmin Lt; RTI ID = 0.0 > a < / RTI > Such plasminogen activators are classified as allergies, fever, local hemorrhages, short half-lives and expensive drugs, and thus they are limited to thrombosis treatment agents. Streptokinase and urokinase are exogenous enzymes that convert plasminogen to plasmin. They contain side effects such as fever, allergy, local hemorrhage and hypotension, and are categorized as very expensive medicines. And it has not been used to prevent thrombus formation in the body.

Therefore, studies on natural plant-derived substances that are safe and have excellent blood circulation improvement activity are being actively conducted, and it is desired to develop a formulation that is useful for improving blood circulation in traditionally proven natural substances rather than synthetic substances that cause side effects May be more preferable.

Korean Registered Patent No. 10-1158739 (June 15, 2012) Korean Patent No. 10-1259499 (Apr. 24, 2014) Korean Patent No. 10-1558524 (Oct. 1, 2015)

The inventors of the present invention have conducted intensive studies to develop a functional material on natural materials based on a long experience of herbal medicine. As a result, the inventors of the present invention have found that by using various herbal medicinal herb components, useful functionalities and side effects And the composition prepared by using the composition optimized by taking into consideration the taste of the product is suitable for the treatment of various cardiovascular diseases including improvement of blood circulation as well as the side effects Thus, the present inventors have found that the present invention can be effectively used for a patient who needs it.

Accordingly, it is an object of the present invention to provide a herbal composition which efficiently extracts and mixes various herbal medicinal herb components and fries or improves them to optimize the content of the active ingredient, the taste of the product, Which is formulated into tablets, pills, tablets, capsules, injections, pouches, drinks, drinks, and the like.

The object of the present invention as described above is to provide a method for producing a medicinal herb including five kinds of animal medicinal materials including water quality, anthrax, horsetail, stigma and pore, one kind of mineral herb medicine including alfalfa, The present invention can be achieved by an agent for improving blood circulation characterized in that it is manufactured from 18 kinds of plant herbal medicines, including Hwanggi, Noni, Sansa, Angelica gigas, Veterinary medicine, Chaga mushroom, Ginseng, Rhubarb,

The above object can be attained by, in a further aspect, a method of preparing a medicament by mixing and extracting whole of each herbal medicine after an individual pretreatment at an appropriate blending ratio, wherein the raw material content of each ingredient is 900 to 1,800 parts by weight of water quality, 900 to 1,800 parts by weight 900 to 1,800 parts by weight of wheat germ, 900 to 1,800 parts by weight of germanium, 900 to 1,800 parts by weight of sprout barley, 900 to 1,800 parts by weight of raw wheat germ, 600 to 1,200 parts by weight of gingerbread, 600 to 1,200 parts by weight of balsam, 600 to 1,200 parts by weight of noni, 600 to 1,200 parts by weight of antler, 600 to 1,200 parts by weight of antler, 600 to 1,200 parts by weight of Angelicae japonica, 600 to 1,200 parts by weight of crawfish, 450 to 900 parts by weight of crab, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of rhubarb, 300 to 600 parts by weight of cornstalks, 300 to 600 parts by weight of cornstalks, 300 to 600 parts by weight of cornstalks, And 200 to 400 parts by weight of cerebrospinal fluid. The.

The agent for improving blood circulation according to the present invention can be useful for prevention and treatment of diseases including hyperlipidemia, atherosclerosis, cerebral hemorrhage and angina pectoris, and can be used for improving blood flow, improving vasculature, improving vascular endothelial function, Dementia, palpitations, convulsions, and pain relief, so it can be used appropriately for patients who require it.

BRIEF DESCRIPTION OF THE DRAWINGS FIG.
2 is a graph showing the result of analysis of degradation of mouse blood clot of blood circulation improving agent.
FIG. 3 is a result of TBA plate assay of herbal preparation. FIG.
4 is a graph showing the antihyperlipidemic effect of oriental herb preparations in hyperlipemia-induced rats.
FIG. 5 is a graph showing the results of the antithrombotic effect assay of oriental herb medicine.

The present invention relates to a process for the production of a medicament for the production of a medicament for improving the quality of a medicinal substance, The present invention relates to a method for producing a herbal medicine which comprises extracting and mixing various herbal medicines of various kinds including herbal medicine, And which is formulated into pills, tablets, capsules, injections, pouches, drinks, beverages and the like.

In a further aspect of the present invention, the herbal medicines are prepared by mixing and extracting the whole of the herbal medicines at a proper mixing ratio after individual pre-treatment, and the raw material content of each ingredient is 900 to 1,800 parts by weight of water quality, 900 to 1,800 parts by weight 900 to 1,800 parts by weight of wheat germ, 900 to 1,800 parts by weight of germanium, 900 to 1,800 parts by weight of sprouts barley, 900 to 1,800 parts by weight of raw wheat germ, 600 to 1,200 parts by weight of guinea fescue, 600 to 1,200 parts by weight of balsamer, 600-1,200 parts by weight of noni, 600-1,200 parts by weight of fenugreek, 600-1,200 parts by weight of antler, 600-1,200 parts by weight of Angelica giganteus, 600-1,200 parts by weight of strawberry, 450-900 parts by weight of crab, 300 to 600 parts by weight of rhizome, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of rhubarb, 300 to 600 parts by weight of rhizome, 300 to 600 parts by weight of corn, 300 to 600 parts by weight of corn, 200 to 400 parts by weight of corn, Wherein the composition contains 200 to 400 parts by weight of cerebrospinal fluid.

Each individual component will be described below.

The water leek is a dried leech (Hirudo nipponica Whitman) and a horse leech (Whitmania pigra Whitman), which is caught between summer and fall, soaking the soil in the water and then killing it in lime or alcohol It dries. Water quality acts on liver and cysts, promotes blood circulation, eliminates eosinophilia, smoothes menstruation, makes urine come out and aborts, and hirudin contained in leeches is anticoagulant.

The dried bloom is a dry lacquer shell, which removes the most powerful of the botanical herbs. Reduce ejaculation, stopping menstruation, acid healing. It is used to make the small intestine well and to eliminate roundworms.

White algae is a medicinal product made of crystals of minerals (alumen) processed with mineral. It can heal chronic gastrointestinal diseases and stomach and duodenal ulcers. When you take it, it gets healed, acute hepatitis becomes swollen and yellow, It is effective. Your wife's uterine dewatering and shrinking effect is obtained, and the effect of germicidal action and convergence is obtained. In addition, the bactericidal action of psoriasis, psoriasis, and lymphatic effusions are effective. It is a medicine that releases my detox and firmness.

The law means rice washed (rice soot) and neutralizes the other ingredients added. In the present invention, rice is poured into water and washed for 30 minutes, washed, and washed three times.

Sprout barley refers to a young plant that grows about 10 to 15 cm in length with buds planted in barley. It is known as a good source of nutrients because of high content of inorganic substances such as calcium, magnesium and potassium as well as a high content of vitamin B1 and vitamin C, and also has antioxidant, anti-inflammatory and anti-cancer saponin saponarin, lutonarin, and isovitexin, and thus their industrial applicability as health functional foods and medicines has increased. This ingredient has the function of alleviating or neutralizing the toxicity caused by the herbal medicines such as Gunpowder.

The seed barley may be grown in the open field or may be obtained by dipping the barley in water for one day, then removing the moisture and preventing water loss, germinating the barley for 10 to 14 days have.

Rehmannia glutinosa is a rhizome of Rehmannia glutinosa Libosch which is a Scrophulariaceae plant and its main components are Catalpol, Verbascose mannitol, Glucose, Mannonotriose Maninotriose, and vitamin A (Vitamin A). It is cold, flavorless, poisonous, free of all heat, breaks blood, moans, cheeks and blood, and is used to control blood (blood).

It is a word that leads to licorice which alleviates the weakness and slightly changed by burning in fire. It is warm when the licorice is baked. It is used to show the masturbation, to help the heart, to show the symptom of pulsating the heart with the unstable pulse due to lack of planting. It is used to stabilize the mind when the heart rhythm is short, Depression, hysteria). It also acts on the lungs to remove the follicles, stop coughing, relieve spasm of the stomach, cramps in the limb muscles, relieve pain, and neutralize the drug when used in combination with other medications.

Bongseonjae is also known as acute, and Seongseonhwa is a seongseongseong liquid action is the contraction of the uterus, contraceptive action (ovulation inhibition, oxygen contraction) is. The application writes seeds in mouth treatments, menstrual irregularities in motion therapy. In addition, the stem is a spine, and it is used for pain, inflammation, rheumatism, rheumatism, bruise, menopause. In the private sector, it is known to treat the symptoms such as habitual joint pain, dysmenorrhea, lymphadenitis, and poisonous bites due to the fact that the seonhwa has the efficacy of blood clotting, analgesia, and swelling.

Astragali Radix is a perennial herb with roots of dicotyledonous roots and roots, and it is derived from the dried roots. Hwanggi is widely distributed in Europe, such as Korea, China, Asia, Russia and Bulgaria. In Korea, it is widely used in about 300 kinds of prescriptions such as Hwanggijangbangtang, Hwangmyeong Geootang, It is an important medicinal product. The main ingredients are isoflavonoid and triterpene saponin, and also contains canavanine and phenol glycoside, and it has diuretic effect, blood pressure lowering effect, tonic action to show the group, cellular and humoral immunity enhancement, anti-inflammatory action, liver and spleen And the reduction of alkaline RNase activity of the cells.

Noni is the Chinese name for 檄 樹 or sea bream, and its scientific name is Morinda Citrifolia. It is a Mongolian madder and Noni plant. Southeast Asia Vietnam, East Malaysia and the South Pacific, and Hawaii is the main fruit of the country. It is made with dried powder and it sells and it does not smell at this time. It plays a role in catching chronic inflammation in our body. In other words, if inflammation or tissue damage occurs in our body, the serum CRP level is slightly elevated. However, when the CRP level is less than 0.5 mg / dl, it is normal and there is no chronic inflammation, vascular atrophy, .

Sansa is a fruit of hawthorn tree containing diverse organic acids, flavonoids and vitamin C, and has the effect of strengthening blood vessels and cardiac function. It is prescribed for postpartum abdominal pain, hangover, dryness, indigestion and chronic diarrhea.

Formica truncicola is a species of ants that belongs to the family Antler, whose body is light reddish yellow and has light yellow hairs throughout the body. Fire ants live in rotten trees, soil, and stones in Korea, Japan, China, Taiwan, and Russia, and do not live under live trees. Fireworms are widely distributed inland in Korea and fewer in the south. Currently, there are about 60 species of ants in 1, 26, and 60 species including ants and fire ants in Korea.

The anemia is known as a fire ant, and its efficacy is known to be effective for joint pain, cardiovascular disease, respiratory system bronchitis, urinary system, digestive system, nervous system, pulmonary tuberculosis, It is an important medicinal drug that has been used mainly when the wound is not healed.

Angelicae Gigantis Radix is a dried perennial roots of Angelica gigantis Radix, a perennial paste that is very sweet and tasty. Angelica contains mainly coumarin-based decursin, decursinol, decursinol angelate and the like. Decker Shin and Decker Cynol are known to have sedative, analgesic action, antitumor action, antitumor action, brain cell protective action, and especially anti-platelet aggregation, which is effective for vascular treatment. In one room, Angelicae is used mainly for the recovery of anemia, women's diseases and postpartum of pregnant women. Besides, it is known that it is effective for severe cough and swelling. In addition, it not only strengthens the uterus but also regulates the physiology to remove waste products such as hemorrhoids, improves the poor circulation, improves the color deficiency, helps to recover the prenatal and postnatal blood, In addition, the circulation of blood smooth, blood purification and exercise of the intestines to stimulate the symptoms of cold hands and feet can be said to improve.

Crawfish is a dried up worm of Eupolyphaga sinensis Walker, which is caught in the subterranean waters and is caught in summer, dipped in boiling water, or brine and dried in the sun. It is known to work on heart, liver, and parenchyma, and it is effective in eliminating hemorrhoids and enemies, making gyeongmaek well and connecting broken bones.

Lumbricus is a dried earthworm (Pheretima communisima Goto et Hatai), an earthworm (Mrgascilecidae) that catches earthworms in the fertile wetlands from spring to autumn, cleanses the ground, flushes the soil, It is dried. Jobs lower the fever, calm the seizures, urinate well, relieve parasites and remove poisons. It is known that earthworms contain persistent hypotensive effects such as lumbritin, lumbrifitin, lumbrifebrine, hypoxanthin, and bronchial smooth muscle relaxant, toxins. have. It is also used for jaundice, jaundice, dysuria, joint pain, hemiplegia, bronchial asthma, hypertension, and lower back pain.

In the autumn, the roots are washed in water and dried in the sun. Vaccine acts on liver, parenteral, and menopausal, and it shows blood, it makes the pain and sweat nice, it bleeds, it makes liver and makes urine come out well. In pharmacological experiments, peoniflorin exhibits sedation, analgesic action, antipyretic action, antipyretic action, anti-inflammatory action, anti-ulcer hypotensive effect, coronary vasodilatory action, It is known to exhibit epidermal, anti-inflammatory, and hemostatic effects.

Inonotus Obliquus is a mushroom that grows on birch trees in the northern high latitudes of Siberia. It is said that the residents who eat it like tea and coffee have few gastrointestinal cancer patients and diabetic patients. The active ingredient of chaga mushroom extract is polysaccharide , Flavonoids, triterpenoids, inositol, cholic acid, and alkaloids, and exhibit high antitumor activity, antitumor activity, hypoglycemic activity and SOD activity in the gastrointestinal tract.

Ginseng (ginseng, Panax ginseng) is a plant of the ginseng and ginseng, and its roots are dried. Ginseng looks at the joy, does not dry the body, it works to cause blood to bloom. The property is a little warm, tasty, a bit spicy, not poisonous. It is a medicinal herb that is known to be effective in the central nervous system, immune system, cardiovascular disease, endocrine system, anti-aging and the like. In particular, in the case of cardiovascular diseases, it is known that ginseng has actions on cardiac action, myocardial ischemia, blood vessel and blood pressure, and blood and hematopoietic system.

Rheum palmatum Linne is the root and rootstock of Polygonaceae and has been removed. The effect of excessive heat accumulated in the body to lower the skin, constipation, constipation, hypertension, treatment of hyperemia and pain. It is also used for early symptoms of dysentery. It is also effective for fever, nosebleeds and acute jaundice due to heat. It is widely used for symptoms such as fever, pain, severe redness, and hot burns. In addition, when the flour is applied to the bronchial flora which is generated in winter, it is excellent in anti-inflammatory action and the wound is easily healed.

Persicae semen (Scientific name: Prunus persica (L.) Batsch) is used as a medicinal herb by drying mature seeds of peaches. It has the effect of activating bovine leukocyte It can be applied to the symptoms caused by most blood vessels (瘀血 inhibition).

Cinnamomum cassia is a dry matter of camphor tree and cassava. Cassia is composed of cinnamon, guinea broccoli, broiler chickens,柳 桂: Cassia tree branch), among which the outer part of the Cassia bark is scraped off and only the inner part is used. It is known that there is efficacy of rejuvenating blood (补阳 活血), reign of fever, and the effect of healing ego.

Pyridoxine refers to the whole root of the extract, and the active ingredient of Pyridoxine is essential oil containing atractylonen, carotene, inulin, vitamin, borneol and the like. It is a medicinal herb known for its strong stomach, and it is recorded in the Fragments of the Orphanage that it becomes longevity and all kinds of illnesses will be longevity if it eats out roasted roots, The roots of rosemary warms the stomach, strengthens the stomach, improves the appetite, stabilizes the fetus, stops diarrhea and makes urine come out well. The efficacy of liquor is promoted secretion of digestive enzymes, continuous diuretic effect and soothing action, and it is excellent for chronic digestion deficiency, enteritis, diarrhea and appetite promotion because it has dryness and digestive action. Especially, it is effective for people who do not have appetite after the illness and the whole body is weak and sweat a lot, and diuretic action is also used for swelling of the kidney and edema of the pregnant woman.

Scolopendra subspinipes mutilans is a dry form of Wangzine, an animal belonging to the king's family. Its pharmacological effects include anticonvulsive action, cardiovascular action, antibacterial action, anticoagulant action, anti-cancer action have.

The juvenile cerebrospinal fluid (Dryobalanops aromatica Gaertner) is cut by cutting the stem or branch of the water vapor distillation of the solution obtained by cooling the lumps collected in the shade dried, heart, menopause, pneumoperitoneal action. The brains clear the mind, lower the fever, calm the swelling and stop the pain.

Hereinafter, the preparation of the herbal preparation according to the present invention will be described. Fig. 1 is a process flow chart of the herbal preparation of the present invention.

As shown in Fig. 1, there are five kinds of animal medicines including water quality, anthracnose, rodent, locus, and pore, one kind of mineral herb medicine including alfalfa, Eighteen plant herbal medicines, including Hwanggi, Noni, Sansa, Angelica gigas, Veterinary medicine, Chaga mushroom, Ginseng, Rhubarb, And may be prepared by extraction.

And more preferably five kinds of animal herbal medicines including water quality, anthrax, horsetail, stigma, and pore, one kind of mineral herbal medicine including alpine, and one kind of mineral herb medicine including dried alfalfa, alfalfa, 18 kinds of phytomedicines, including Sansa, Angelica gigas, Veterinary medicines, Chaga mushrooms, Ginseng, Rhubarb, Rhodes,

a) a step of mixing the herbal medicines individually collected and pretreated, followed by natural drying;

b) mixing each of the dried herbal medicines at a predetermined ratio, and separately pulverizing the granules to a particle size of 80 to 200 mesh;

c) firing the pulverized herbal medicines at 70 to 80 ° C for 2 to 10 minutes with stirring or at a temperature of 100 to 105 ° C for 2 to 10 minutes; And

d) cooling the herbal medicines treated by frying or boiling;

e) adding 10 to 25 times of purified water to the cooled herbal medicine on the basis of weight, and extracting the extract at a temperature of 0.5 to 0.6 atm and a temperature of 70 to 80 ° C for 60 to 72 hours to obtain an extract;

f) filtering the extract of the above step with a filter paper to remove foreign matter;

g) filtering the foreign matter and adding purified water of 10 to 25 times the weight of the solid to the remaining solid, extracting the extract at a temperature of 0.8 to 0.9 atm and a temperature of 80 to 90 ° C for 60 to 72 hours to obtain an extract;

h) filtering the extract of the above step with a filter paper to remove foreign matter;

i) filtering the foreign substances and concentrating the obtained filtrates to a solid of 40 or more at a temperature of 50 to 65 DEG C for 4 to 8 hours under a reduced pressure of -0.08 to -0.09 MPA;

j) drying the concentrate obtained in the above step under reduced pressure at a temperature of 55 to 65 DEG C for 24 to 48 hours; And

k) pulverizing the dried material obtained in the above step to 120 to 200 mesh, and then extracting the dried material to formulate it.

In this process, purified water or water may be used as an extraction solvent, and at least one selected from a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.

More specifically, the herbal medicine is preferably subjected to hot air drying at a temperature of 55 to 65 ° C for the purpose of pre-treatment.

Then, each of the dried herbal medicines is mixed at a predetermined ratio, and then ground to a particle size of 80 to 200 mesh. Finely milled into about 150 meshes.

Then, the ground herbal medicines are roasted at 70 to 80 ° C for 2 to 10 minutes under agitation or treated at a temperature of 100 to 105 ° C for 2 to 10 minutes and then cooled.

In the above process, roasted or roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roasted roast roast Or roasted at a temperature of 100 to 105 ° C for 2 to 10 minutes. Here, fry means roasting and roasting means heating.

Then, 10 to 25 times as much purified water as weight is added to the dried herbal medicine, and the extract is extracted at a temperature of 0.5 to 0.6 atm and a temperature of 70 to 80 ° C for 60 to 72 hours.

As a result of the experiment, it was found that the herbal medicinal herb extract was more efficient than the high temperature extraction at 60 ℃ ~ 70 ℃ for low temperature or medium temperature extraction. In addition, the extracts extracted from 60 ℃ ~ 70 ℃ showed the highest contents of various oriental medicinal ingredients. When the extracts were extracted above 80 ℃, the content of active ingredients decreased.

The extraction time for extraction of oriental medicinal herb was the highest when the extract was extracted for 72 hours, and the extract solid content and the active ingredient content tended to be lower when the extraction time exceeded 72 hours.

On the other hand, the amount of the extraction solvent is good when it is extracted by 10 ~ 25 times of the experiment results for standardization of the amount of the solvent, and when the energy efficiency and economical efficiency are considered, extraction by adding 10 times the solvent may be most effective. The extraction pressure is preferably carried out under the condition of 0.5 to 0.6 atm.

The extract of the above step is filtered with a filter membrane or a paper filter to filter out foreign matter and filter it under conditions free of debris.

Then, 10 to 25 times as much purified water as weight is added to the remaining solids, and the extract is extracted at a temperature of 0.8 to 0.9 atm and a temperature of 70 to 80 ° C for 60 to 72 hours to obtain an additional extract. Similarly, To extract the active ingredients as much as possible.

Then, the filtrates obtained by filtering the foreign matters are combined and concentrated at a temperature of 50 to 65 ° C under a reduced pressure of -0.08 to -0.09 MPa for 4 to 8 hours to a brix of 40 or more. As a result of various test conditions, the yield was the best when concentrated under reduced pressure of -0.08 to -0.09 MPa using a vacuum condenser.

The concentrate obtained in the above step is preferably dried under vacuum at a temperature of 55 to 65 DEG C for 24 to 48 hours at a low temperature.

Next, as the step of pulverizing the dried material obtained in the above step to 120 to 200 mesh, it is pulverized to a size of preferably about 150 mesh using finishing mill or the like.

After the drying step or the pulverizing step, a sterilization step may be further included in accordance with the product conditions.

As an alternative method, it is also possible to treat the animal medicinal material and the phytogenic medicinal material separately. First, five kinds of animal medicinal herb including pre-treated water quality, antimicrobial, insecticide, ,

a1) naturally drying the mixed herbal medicine;

b1) individually pulverizing the dried animal herbaceous material to a particle size of 80 to 200 mesh;

c1) crushing the crushed animal herbaceous material with stirring at 70 to 80 ° C for 2 to 10 minutes or at a temperature of 100 to 105 ° C for 2 to 10 minutes; And

d1) frying or boiling, and cooling the animal herb medicine treated.

In addition, 18 kinds of phytosanitary products including crudes, crustaceans, sprouts, bamboo shoots, bamboo shoots, bamboo shoots, bongsikjae, hwanggi, noni, sansa, angelica, white peony, chaga mushroom, ginseng, rhubarb, Can be extracted individually or in combination,

a2) adding 10 to 25 times of purified water to the phytogenic herb medicine without drying or drying and extracting the extract at a temperature of 0.5 to 0.6 atm and 70 to 80 ° C for 60 to 72 hours to obtain an extract;

b2) filtering the extract of the above step with a filter paper to remove foreign matter;

c2) filtering the foreign matter, adding purified water of 10 to 25 times the weight of the solid to the remaining solid, extracting the extract at a temperature of 0.8 to 0.9 atm and a temperature of 80 to 90 DEG C for 60 to 72 hours to obtain an extract;

d2) filtering the extract of the above step with a filter paper to remove foreign matter;

e2) filtering the foreign substances and concentrating the obtained filtrates to a solid of 40 or more at a temperature of 50 to 65 DEG C for 4 to 8 hours under a reduced pressure of -0.08 to -0.09 MPA;

f2) drying the concentrate obtained in the above step under reduced pressure at a temperature of 55 to 65 DEG C for 24 to 48 hours; And

g2) The step of pulverizing the dried material obtained in the above step to 120 to 200 mesh may be preferable.

The present invention has been made with a view to develop a preparation having various side effects and having low side effects and improving blood circulation function. The present invention has been made to provide various herbal medicinal herb ingredients and their combination ratio, efficient extraction conditions of each ingredient, And the composition ratio of the preparation according to the present invention is set according to a long study result in consideration of the effective dose and side effects of each component and when the ratio is out of the range, There is a fear that the desired synergistic effect is not exhibited and the effect is lowered.

The blood circulation improving agent of the present invention can be effectively used as a medicine or a health functional food for preventing and improving diseases including hyperlipidemia, arteriosclerosis, cerebral hemorrhage and angina.

The anti-platelet agent of the present invention exhibits a thrombolytic activity, an antihyperlipidemic effect, a hemolytic activity, a coronary blood flow improvement, an improvement in an animal model of local cerebral ischemia, and a function of inhibiting platelet aggregation without toxicity and side effects, , The state, and the individuals who are suspected of suffering from an abnormality.

The pharmaceutical and food formulations according to the present invention can be formulated in the form of oral preparations such as pills, pouches, or drinks, or injections according to a conventional method in the art.

Particularly, in the case of manufacturing with a pellet, the respective materials are mixed in a mixer such as a double cone mixer for 20 minutes or more, and then combined using a coater. The mixture is cooled using a conventional ventilator, dried at about 30 캜, Which can be prepared by coating and packaging.

For coating, it may be preferable to dissolve hydroxypropylmethylcellulose in a 95% alcohol solution followed by spray coating. It is preferable to dry and coat the product at a temperature of 30 ± 2 ° C for at least 1 hour to a water content of 5% or less.

The dosage of the medicament and the food preparation of the present invention is appropriately set in accordance with the form of the preparation, the method of administration, the purpose of use, and the age, weight and symptom of the individual to which the composition is applied. For example, 25 mg to 5,000 mg / kg per day. Of course, since the dosage varies depending on various conditions, the amount may be less than the above amount, or may be more than the above range.

The pharmaceutical preparation according to the present invention can be formulated in combination with a known pharmaceutical carrier. Generally, the active ingredient is mixed with a pharmaceutically acceptable liquid or solid carrier and, if necessary, a solvent, a dispersant, an emulsifier, a buffer, a stabilizer, an excipient, a binder, a disintegrant, , Granules, powders, powders, capsules, and the like, liquid solutions such as liquid solutions, suspensions, and emulsions. It may also be a dried product which can be made into a liquid phase by the addition of a suitable carrier before use.

The pharmaceutical carrier can be selected according to the above dosage form and formulation, and in the case of an oral preparation, for example, starch, lactose, white sugar, mannitol, carboxymethyl cellulose, corn starch, inorganic salt and the like are used. In the preparation of an oral preparation, a binder, a disintegrant, a surfactant, a lubricant, a flowability promoter, a mating agent, a colorant, a perfume, and the like may be further blended.

The food preparation according to the present invention may be prepared by adding a conventional adjuvant or additives or a sweetener such as honey, malt, glycerin, red ginseng, cordyceps, grapefruit extract, licorice, pulp leaf powder, One or more components selected from the group consisting of glucose, sucrose, glucose, glucose, glucose, glucose, glucose, glucose, glucose, glucose, glucose, Can be increased. They are suitably used at about 50-80% based on the total weight of the formulation of the present invention.

<Examples>

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example 1: Preparation of blood circulation improving agent containing herbal medicine extract

Each of the animal herbal medicines purchased in the market (1,200 g of water quality, 1,200 g of alfalfa, 1,200 g of alfalfa, 1,200 g of ginseng, 1,200 g of sprouts, 1,200 g of raw persimmon, 900 g of ginseng, 900 g of balsam, 900 g of ginseng, The herbal medicines of 900g, 900g, 900g, 700g, 700g, 700g, 700g, 500g, 500g, 500g, 500g, 500g, 300g and 300g were mixed and dried naturally Next, the mixture was ground to a particle size of 150 mesh, and the ground herb medicine was fried at 80 DEG C for 5 minutes with stirring, then allowed to stand at room temperature and then dried. 500 g of rice was poured into 500 g of rice, washed for 30 minutes, washed three times, and washed to collect 1200 g.

After mixing all of the dried herbal medicines, 200 liters of purified water was added to the mixture and the mixture was extracted at a temperature of 0.5 atm and 80 ° C for 72 hours. The extract was filtered with paper filter paper, and the filtrate was collected. 200 L of purified water was added to the remaining solids, and the solution was then extracted at a temperature of 0.9 atm and 80 ° C for 72 hours. The extracts were filtered through paper filter paper and the filtrates were combined and concentrated under reduced pressure of -0.08 MPA at 80 ° C for 4 hours. The brix of the solid was 50. Subsequently, the concentrate was dried under vacuum at a temperature of 55 DEG C for 24 hours at a low temperature, and a preparation for improving blood circulation containing herbal medicine extract was prepared.

Example 2: Production of pills

7% of the blood circulation improving agent of Example 1 (hereinafter referred to as weight), 23% of malt powder, 3% of millitared caterpillar powder, 7% of citric acid, 10% of sorbitol, 35% of xylitol and 15% of oligosaccharide After 25 minutes of mixing, they were combined using a coater, and the mixture was air-conditioned. After drying at about 30 캜, hydroxypropylmethylcellulose was dissolved in 95% alcohol, spray-coated, and dried 30 ㅁ After drying for 1 hour at 2 ° C with moisture content of 5% or less, packed (PS) to prepare a pellet having an amount of 3.5 g.

Example 3: Tablet formulation

Preparation for improving blood circulation (Example 1) 25 mg

Lactose 60 mg

Starch 15 mg

Magnesium stearate 15 mg

Crystalline cellulose 15 mg

Tablets may be prepared by conventional methods and one preferred embodiment is in the form of tablets or soft capsules with conventional enteric coatings (e. G., Hydroxypropylmethylcellulose phthalate), sugar coating or coatings.

Example 4: Preparation of beverage

20 g of citric acid, 17 g of aspartame and 30 g of vitamin C were added to 100 g of the blood circulation improving agent obtained in Example 1, and the remaining was added with purified water to make 10 L of drinking water and pasteurized. As a result, the beverage of the present invention obtained favorable responses to conventional beverage products in terms of mouth feel, taste, and overall evaluation.

Example 5: Preparation of injection

20 mg of the hemostatic composition of Example 1

180 mg mannitol

Sterile sterilized water for injection 2800mg

(3 ml) per 1 ampoule according to the usual injection method.

Example 6: Manufacture of a pouch

Pouches Pouches were prepared by an automatic filling machine so that 10 g of vitamin C, 10 g of aspartame and 50 g of purified water were contained in 40 g of the blood circulation improving preparation obtained in Example 1.

Example 7 Acute Toxicity Test

The toxicity test of the blood circulation improving agent according to Example 1 was performed on the experimental animals and the toxicity thereof was observed. The experimental animals were male and female SD male rats weighing 105 ± 4 g and 95 ± 3 g, respectively, at 4 and 5 weeks of age, and tested using distilled water as the negative control and the preparation of the present invention. First, the rats were incubated in a laboratory incubation box with a light and dark cycle of temperature 22 ± 2 ° C., relative humidity 53 ± 2% and fluorescent light (09:00 lit-18: 00 off) and illumination conditions of 150-300 Lux, The animals were divided into groups in which the average body weight was matched. Animals were orally administered at a dose of 20 ml / kg once a day for 14 days, and the change in general condition, poisoning symptom , Mobility, appearance, autonomic nervous system, weight change, and presence of dead animals.

According to the experimental results, there was no significant difference in the body weight during the experimental period, but there was no significant difference. The highest concentration of 20 ml per kg, which is the maximum amount of sample to be administered to the test animal, was selected, LD ₅₀ was more than 20 ml / kg BW because of the inability to calculate the lethal dose. As a result, it was proved that the formulation was not toxic.

Example 8: Degradation analysis of blood clot

0.5 ml of each of the tubes was subjected to 45 minutes of reaction at 37 ° C to form clots. After the clots were formed, the serum layer was completely removed and the clot mass was measured. The herbal preparation of Example 1 was reacted to confirm the blood clot decomposing effect.

The results are shown in Fig. Fig. 2 is a result of analysis of degradation of a mouse blood clot of a preparation for improving blood circulation according to the present invention. Fig. The control group contained only phosphate buffer and the anti-haemostatic agent contained 100 μg.

In the degradation analysis of the mouse blood clot, 100 μg of the herbal medicine extract preparation (Example 1) degraded the fibrin clot by about 82%. Mouse blood clot The results of in vitro experiments showed that thrombolytic activity, which can directly decompose the clots formed in the blood of the herbal medicine herbal medicine, can be confirmed.

Example 9: Lipid degradation effect

(1) TBA plate assay

10 μl of the herbal extract preparation of Example 1 was immersed in a TBA (tributyrin agar) plate containing 0.1 ml of tributyrin and 0.1 g of agar, followed by immersion, followed by reaction at 37 ° C. for 12 hours, Respectively.

The result of TBA (tributyrin agar) plate assay of the herbal extract preparation is shown in FIG. 3 is a result of TBA plate assay of the herbal extract preparation. The buffer contained only phosphate buffer, and the herbal extract formulation contained 10 μg. From the above results, 10 μg of the herbal extract preparation was able to decompose lipids and identify a clean zone around the rings. This experiment confirmed that the herbal extract formulation directly decomposes the lipid components.

(2) Lipid degradation activity assay using lipidase synthesis substrate

Enzyme activity was measured by a colorimetric method (Stuer et al., 1986) using 4-nitrophenyl palmitate and 4-nitrophenyl butryrate. 1 mg of the herbal extract preparation of Example 1 was dissolved in 1 ml of 50 mM phosphate buffer solution (pH 6.0), and 10 μl of a substrate solution (100 mM p-nitrophenyl butyrate (pNPB) dissolved in 2-propanol) Lt; 0 &gt; C for 30 minutes. The supernatant was recovered by centrifugation at 2000 x g for 5 minutes at 4 ° C and the activity was measured. Lipolytic enzyme activity was confirmed by measuring the amount of p-nitrophenol produced at 410 nm. The definition of one unit of enzyme is the amount of enzyme that produces 1 μM p-nitrophenol per minute. The results are shown in Table 1 below.

temperament Lipolytic activity (mU / ml) p- nitrophenyl butyrate 5470 p- nitropyryl palmitate 2780

As can be seen from the results shown in Table 1, the herbal extract preparation exhibited lipolytic activity of 5470 mU / ml in the p- nitrophenyl butyrate substrate and 2780 mU / ml in the p- nitropynyl palmitate substrate. This experiment confirmed that the herbal extract preparation had the same activity as lipase, which is an enzyme that directly decomposes lipid components, and confirmed high activity in each substrate.

Example 10: Anti-hyperlipemia test

Blood was collected from the rat via the tail vein. The blood was left at room temperature for 30 minutes, centrifuged at 3000 rpm for 20 minutes, and the serum was extracted and stored at -30 ° C until analysis. We analyzed the effects of total cholesterol, TG, HDL-C, LDL-C and VLDL-C in hypercholesterolemic patients. Friedewald Were analyzed by the concentration calculation method.

The results of analysis of the effect of anti-hyperlipemia by feeding diets supplemented with the herbal extract preparation (Example 1) to hyperlipemia-induced rats are shown in Fig. 4 is a graph showing the antihyperlipidemic effect of the herbal extract preparation in hyperlipidemia-induced rats. C is the plasma of normal rats as a control, NC is the plasma of hyperlipemia-induced rats as a negative control, and the sample is plasma of hyperlipemia-induced rats fed with herbal extract preparation (1 mg) as an experimental group.

As shown in FIG. 4, the total cholesterol and HDL-cholesterol levels of the control group (N, general diet) fed only with the general diet were 112.0 mg / dL and 67.2 mg / , LDL-cholesterol (low-density lipoprotein cholesterol) was 16.2 mg / dL, VLDL-cholesterol (low-density lipoprotein cholesterol) was 23.3 mg / dL and TG (triglyceride) was 114.2 mg / dL.

The total cholesterol, LDL, and VLDL levels in the negative control group (NC, hyperlipidemia-inducing group) fed with high-cholesterol diet were 141.1 mg / dL, 49.6 mg / dL and 26.5 mg / dL, , And TG (triglyceride) levels were measured as 138.3 mg / dL. The total cholesterol, HDL, LDL, VLDL, and TG of normal group fed diets were increased by 26.0% and 26.2%, respectively. LDL increased by 285.1% and VLDL and TG increased by 13.7% and 21.1%, respectively.

The total cholesterol level was 108.5 mg / dL, the HDL level was 70.3 mg / dL, the LDL level was 18.3 mg / dL and the VLDL level was 16.1 mg / dL in the sample group in which the hyperlipemia- mg / dL, and the TG value was measured as 95.3 mg / dL.

 Total cholesterol, HDL, LDL, VLDL and TG levels were restored to normal levels, and LDL levels were significantly lower in the control group and in the control group fed the normal diet and in the control group fed the high cholesterol diet. The HDL level was significantly elevated from 48.2 mg / dL to 18.3 mg / dL, and the recovery effect of the herbal medicine diet was confirmed.

In addition, AI (atherogenic index, total cholesterol-HDL cholesterol) / HDL-cholesterol), HDL / LDL ratio and TC / HDL ratio were calculated and compared and the results are shown in Table 3.

Item C NC Herbal medicine AI 0.67 1.84 0.54 Total cholesterol / HDL ratio 1.67 2.84 1.54 HDL / LDL ratio 4.14 1.07 3.84

As a result, diets supplemented with herbal extracts were fed to rats that induced hyperlipidemia for 14 days. As a result, it was confirmed that the results of all five items had blood circulation improving effect (hyperlipemia prevention effect) cholesterol / HDL ratio and HDL / LDL ratio.

Example 11 Analysis of Hemolytic Activity Using Rodent Blood

Hemolytic activity assay is an anti-thrombocyte activity assay using a rodent blood clot derived from the blood of an experimental animal through an anti-thrombotic activity assay. The blood clot is formed after natural blood coagulation, It is an experiment to analyze the amount of thrombus and the reaction solution to evaluate the thrombus removal efficacy of the sample.

The blood clot remained after 1 hour of reaction with the herbal extract preparation and the blood clot. The absorbance of the remaining blood clot and the absorbance of the reaction solution were measured to analyze the thrombecting efficacy of the herbal extract preparation.

Blood clots were formed by spontaneous coagulation of 300 μL blood clots of mouse blood in vitro. The degradation of the blood clots was evaluated by incubating the herbal extract preparation sample, positive control t-PA (20 U), and saline-treated clots for 1 hour at 37 ° C. After incubation, the antithrombotic activity was evaluated by color development and the absorbance of the supernatant (blood clot + saline (con), herbal extract preparation or t-PA) was read as absorbance at 410 nm. FIG. 5 is a graph showing the result of the antithrombotic effect assay of the herbal extract preparation. FIG.

The absorbance of 0.455, 0.730, 1.320, 1.350 and 1.388 were observed at 410 nm in the order of 10 ㎍, 20 ㎍, 50 ㎍, 100 ㎍ and 200 ㎍. The absorbance of the negative control group treated with saline instead of the sample was 0.403, and the positive control group t-PA was 0.462 at 10 U concentration, 0.880 at 20 U and 1.380 at 30 U.

The absorbance of 10 μg of herbal extract preparation was slightly lower than that of 20 U t-PA, but higher than that of 10 U t-PA at 50 μg to 200 μg of herbal extract solution.

The antithrombotic activity assay showed excellent thrombolytic ability after the thrombus formation of the experimental animal blood, and showed higher thrombolytic ability than the 10 U t-PA in the extract of 20 μg or more. Therefore, the herbal medicine extract of herbal medicine acts on the blood of the experimental animal and it can be expected to control the coagulation and improve the blood circulation through prevention of excessive thrombus formation.

Example 12: Test for effect on coronary blood flow

Cholesterol diet was administered to the rat at 6 weeks of age for 2 weeks, and the control group was orally administered once daily forcibly with saline and the herbal medicine extract preparation of Example 1 according to the present invention. Two weeks later, the rats were anesthetized with pentobarbital, and hearts were perfused with heart perfusion with Krebs-Henseleit physiological solution at 37 ° C. When the extracted heart was stabilized after 30 minutes, the change of the coronary blood flow was measured with an electric blood flow meter. The results are shown in the following Table 4 as mean ± S.E.M. Statistically significant (P <0.05) compared with control group.

Concentration (mg / kg) Coronary flow (ml / min) Control group 18.8 ± 1.90 Example 1 28.2 ± 1.05

As can be seen from the results of Table 4, the control group was 18.8 ml / min for coronary blood flow whereas the herbal medicine preparation of the present invention was 28.2 ml / min for the concentration-dependent increase.

Example 13: Influence test on the change of degree of neurological deficit

We investigated the effect of local cerebral ischemia on the severity of neurological deficit associated with severe cerebral artery occlusion.

Male rats (250-300 g) were orally administrated once daily as a control group to the saline-treated group and the herbal medicine preparation group of Example 1 according to the present invention. Thereafter, the cervix was dissected while maintaining the temperature at 37 ° C under pentobarbital anesthesia to separate the right common carotid artery, internal carotid artery, and external carotid artery. The common carotid artery and external carotid artery were ligated, and the probe was inserted into the internal carotid artery from the internal carotid artery at the junction of the internal and external carotid arteries and ligated just above the insertion site to close the base of the middle cerebral artery.

The surgical site was sutured and placed in a single cage until the anesthesia was awakened. After 30 minutes, we observed neurological deficit by movement of tail. After closing the middle cerebral artery for 120 minutes, the thread connected to the end of the probe was pulled out about 1 cm and recirculated.

The following table 5 shows the degree of neurological deficit in the forelimb and the degree of paralysis was divided into 0, 1, 2, 3, and 4 grades by observing the symptom of semi-imperfect paralysis.

Severe cerebral artery occlusion Hypoxia Recirculation Control group 2.82 ± 0.21 2.75 ± 0.23 2.70 ± 0.21 Example 1 1.87 + - 0.45 2.07 ± 0.51 1.76 + 0.33

In the control group, the grades were 2.82 ± 0.21, 2.75 ± 0.23, and 2.70 ± 0.21 immediately after surgery, hypoxia and recirculation, respectively, and 1.87 ± 0.45, 2.07 ± 0.45, and 1.76 ± 0.33, respectively.

The following table 6 shows the results of the neurological deficit of the hind legs. Compared to the control group, the administration group according to the invention was significantly improved.

Severe cerebral artery occlusion Hypoxia Recirculation Control group 0.50 + - 0.43 0.244 + 0.26 0.61 + 0.76 Example 1 0.10 0.21 0.01 ± 0.21 0.01 ± 0.30

Example 14 Platelet Aggregation Inhibition Test

Platelet rich plasma (PRP) and platelet poor plasma (PPP) were separated from the blood obtained by anesthetizing male rats (200-300 g) using a centrifuge and the anti - thrombotic effect was measured using Aggregometer. In order to examine platelet aggregation inhibition ability of the herbal extract preparation according to the present invention, thrombin (thrombin-100 unit / ml) was treated as a control group after adding saline solution group and ug / ml of the preparation of Example 1 according to the present invention. The results are shown in Table 7 below.

Concentration (ug / ml) Platelet aggregation inhibition rate (%) Control group 0.01 ± 0.23 Example 1 84.3 ± 0.26

As can be seen from the results of Table 7 above, the efficacy upon treatment with thrombin inhibited 84.3% and showed excellent antithrombotic effect.

The herbal remedy for blood circulation improvement according to the present invention can be used for improving blood circulation, improving hyperlipidemia, testing the therapeutic effect on coronary artery disease, testing the therapeutic effect on the improvement of heart disease, testing the therapeutic effect on brain diseases, It was confirmed that the pharmacological effect was excellent as confirmed by the therapeutic test on diabetes related diseases.

As a result of the above results, the blood circulation improvement effect and the antihyperlipidemic effect were confirmed, so that it can be applied not only to medicines or health-orientated foods, but also to the elderly people in their 60s or older who have entered the age of aging, And to use it as a product to maintain a healthy life such as prevention and suppression of degenerative physical function, and to prevent the prevention of cardiovascular diseases, Can also be used in the healthcare industry.

Claims (5)

It has been reported that the water quality, antimicrobial, insecticide, stomatologic, gut, alfalfa, alfalfa, rice sole, sprout barley, Containing the hot-water extract of the cerebellum,
The raw material content of each component is 900 to 1,800 parts by weight of water quality, 900 to 1,800 parts by weight of crab meat, 900 to 1,800 parts by weight of wheat flour, 900 to 1,800 parts by weight of rice flour, 900 to 1,800 parts by weight of sprout barley, 600-1,200 parts by weight of red ginseng, 600-1,200 parts by weight of green ginseng, 600-1,200 parts by weight of red ginseng, 600-1,200 parts by weight of red ginseng, 600-1,200 parts by weight of noni, 600-1,200 parts by weight of red ginseng, 600-1,200 parts by weight of red ginseng, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of rhubarb, 300 to 600 parts by weight of ginseng, 300 to 600 parts by weight of rhubarb, 300 to 600 parts by weight, 300 to 600 parts by weight, 200 to 400 parts by weight of pores, and 200 to 400 parts by weight of cerebrospinal fluid,
An anti-hyperlipemic activity, an hemolytic activity, an improvement in coronary blood flow, an improvement in an animal model of local cerebral ischemia, and a function of inhibiting platelet aggregation. The method according to claim 1,
Ginseng, rhubarb, ginseng, rosemary, rosemary, and cerebrospinal fluid of the above-mentioned water quality, anemia, Herbal medicines containing
a) a step of mixing the herbal medicines individually collected and pretreated, followed by natural drying;
b) mixing each of the dried herbal medicines at a predetermined ratio, and separately pulverizing the granules to a particle size of 80 to 200 mesh;
c) firing the pulverized herbal medicines at a temperature of 70 to 80 ° C for 2 to 10 minutes under agitation or for 2 to 10 minutes at a temperature of 100 to 105 ° C;
d) cooling the herbal medicines treated by frying or boiling;
e) adding 10 to 25 times of purified water to the cooled herbal medicine on the basis of weight, and extracting the extract at a temperature of 0.5 to 0.6 atm and a temperature of 70 to 80 ° C for 60 to 72 hours to obtain an extract;
f) filtering the extract of the above step with a filter paper to remove foreign matter;
g) filtering the foreign matter and adding purified water of 10 to 25 times the weight of the solid to the remaining solid, extracting the extract at a temperature of 0.8 to 0.9 atm and a temperature of 80 to 90 ° C for 60 to 72 hours to obtain an extract;
h) filtering the extract of the above step with a filter paper to remove foreign matter;
i) filtering the foreign substances and concentrating the obtained filtrates to a solid of 40 or more at a temperature of 50 to 65 DEG C for 4 to 8 hours under a reduced pressure of -0.08 to -0.09 MPA;
j) drying the concentrate obtained in the above step under reduced pressure at a temperature of 55 to 65 DEG C for 24 to 48 hours; And
k) pulverizing the dried material obtained in the above step to 120 to 200 mesh,
Wherein the rice soot is obtained by pouring the same amount of water into rice and then boiling for 30 minutes, followed by washing and repeating the washing three times. The method according to claim 1,
Wherein the agent is in the form of an oral preparation, a pill, a tablet, a capsule, an injection, a pouch, a drink, or a drink. delete delete

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