CN107518399A

CN107518399A - A kind of dietary supplements and its preparation method and application

Info

Publication number: CN107518399A
Application number: CN201710556147.2A
Authority: CN
Inventors: 迈克尔·托马斯·麦克纳马拉
Original assignee: Sunshine Medical Operation Co Ltd
Current assignee: Sunshine Medical Operation Co Ltd
Priority date: 2017-07-10
Filing date: 2017-07-10
Publication date: 2017-12-29

Abstract

The invention provides a kind of dietary supplements, including 65 85 parts of Moringa extracts, 5 12 parts of hydroxytyrosols, 5 12 parts of oleuropeins and 5 11 parts of piperings.The dietary supplements of the present invention can further comprise upper dietetic product and/or pharmaceutically acceptable carrier and/or auxiliary material so that peroral dosage form is made.The preparation method of the dietary supplements of the present invention is to prepare Moringa extract, hydroxytyrosol, oleuropein and pipering according to predetermined ratio；And Moringa extract, hydroxytyrosol, oleuropein and pipering are mixed.The dietary supplements of the present invention has remarkable anti-oxidant and antiphlogistic effects, available for preparing health products and medicine.

Description

A kind of dietary supplements and its preparation method and application

Technical field

The invention belongs to medicines and health protection field, in particular it relates to a kind of dietary supplements and preparation method thereof and Using.

Background technology

Human health is current important research theme.Although having paid a large amount of funds and effort to extend human longevity, It is that the incidences of disease of many serious diseases still persistently raises.These diseases include some most fatal diseases of facing mankind today, bag Include heart disease, cancer, diabetes, hypertension, arthritis, obesity, COPD (COPD) and nervus retrogression disease Disease.

Above-mentioned many diseases can be prevented.Prevention can be predicted according to some factors, wherein several factors with Education, heredity, diet, life style are relevant with environmental factor.

Inflammation and its relation with disease

Inflammation is a natural process；It is the normal components of immune response and is the standard reaction for damage.From For in this layer of meaning, chronic body inflammation is a broader term, relate to have significantly body metabolism state and process A series of body chemistries reaction influenceed and phenomenon.

Inflammation is to human health.Skin burn restores or avoids common cold from being related to inflammation.Therefore, inflammation is very It is beneficial to health in the case of more.

However, cause the inflammation of chronic systemic disease and the presence height correlation of specified disease.Angiocardiopathy, cancer Disease, arterial hypertension, diabetes, metabolic syndrome, hypercholesterolemia, arthritis, obesity and Alzheimer's disease are all Display is relevant with the horizontal abnormality rise of chronic body inflammation.Specific biomarker, such as C reactive protein can be measured (CRP), to prove these inflammation.

In overweight and excessively fat individual excessive stomach fat and interior fat also with the inflammatory molecule of referred to as cell factor Excess generation is relevant, and wherein cell factor in systemic circulation and increases risk.

A most significant inflammatory molecule is interleukin-6 (IL-6) caused by stomach fat.In portal vein High-caliber IL-6 is measured, the vein of wherein portal vein from consumption stomach fat receives blood.In addition, high-caliber IL-6 with The CRP of high blood level is directly related.Oxidative stress also can also increase the presence of other detrimental cytokines, including conversion life The long factor-β (TGF-β) and tumor necrosis factor-alpha (TNF-α).

A kind of most common inflammation mediated disease is artery sclerosis.It is important that inflammatory cytokine compound can change body The integrality of the endothelial cell of blood vessel (heart coronary artery and arteria carotis).The inflammatory reaction then occurred promotes adipocyte Abnormal accumulation in macrophage, macrophage can be accumulated in ductus arteriosus wall, then influence arterial lumens.This symptom is referred to as Artery sclerosis, if unconstrained, the vascular wall internal diameter that can cause to be related to is substantially reduced, and the final end organ tissue that reduces fills Note.

Fat Accumulation is referred to as patch in vascular wall.It is unstable during height inflammatory states or when undergoing oxidative stress Fixed patch in some cases can lost integrity, cause plate to rupture.This phenomenon and blood from the seepage of impaired plate with And finally thrombus (blood clot) formation is relevant.The main reason for Arterial thrombosis is most heart and many apoplexy.

Chronic body inflammation is also relevant with horizontal elevated fasting blood-glucose and related elevated glycated hemoglobin.Internal organ Excessive overweight and excessively fat individual can also produce circulating insulin and be obstructed with stomach fat, and insulin is by pancreas generation and eventually Cause the long-term rise of blood sugar level.This is referred to as type ii diabetes.

The risk that the patient for having chronic body inflammation suffers from malignant tumour can also increase.The most common cancer of some mankind, bag Lung cancer, colorectal cancer, breast cancer, liver cancer, stomach cancer and prostate cancer are included, also can be in the individual for having the chronic body inflammation of high level Middle generation, it is higher than the incidence of disease of healthy individuals.

Researcher has confirmed that the level of inflammation for the patient for suffering from Alzheimer disease and Parkinson's disease is not higher than ill Individual.

Oxidative stress and its relation with disease

In human body, oxidation is the important physiology course of production energy.However, during production energy, also can Produce free radical derived from oxygen.Sometimes the generation of such free radical is uncontrolled and after may being caused to the histocyte of the mankind Continuous injury.

Under its simplest pattern, when the amount deficiency that excessive free radical or anti-oxidizing compounds be present, or two kinds of feelings Condition simultaneously in the presence of, it may appear that oxidative stress, it is potentially to the process of histologic lesion.By unopposed free radical activity Caused infringement is classified as oxidative stress.

Under normal physiological conditions, the atom of all stabilizations has paired outer-shell electron, and to adjacent tissue not With obvious reactivity.Free radical is defined as, containing single not paired electronics, substantially increasing its tissue reactive. Under biotic environment, due to these free radicals or compound may not make any distinction between with neighbouring molecule and cell effect, Therefore this tissue reactive of free radical is probably breakneck.

Free radical, which may produce referred to as " electronic theft " (electron stealing) phenomenon, the phenomenon, causes tissue Aoxidize and destination organization molecule may be made to lose activity.If the concentration of free radical is sufficiently large, these reactions are likely to result in Extensive cellular damage.

According to anti-oxidizing compounds relative availability is neutralized, the degree of tissue damage may relax or reduce.It is such anti- Oxidant directly and radical reaction, and neutralizes their reactivity, and linked groups' toxicity with low degree.

Oxidative stress is also possible to cause oxide-containing chemical material excessive, is referred to as active oxygen (ROS).The drastically liter of ROS concentration It is high that there may be obvious cellular damage.

Oxidative stress may be produced by many reasons, including diet is bad, toxin, radiation, medicine and air pollution.The world Upper multiple regional surrounding airs contain substantial amounts of pollutant.Some air pollutants, such as nitrogen dioxide, itself may be exactly Free radical.The acute and chronic result exposed to this extensive air pollutants is that the oxidative stress in intrapulmonary occurs, as long as Pollutant is inhaled into or into the circulatory system, soon causes extensive system oxidation stress.

The selective oxidation as caused by air pollution may stress again cause more extensive inflammatory reaction, and it may be Cause more free radicals again in vivo.When lacking the antioxidant of sufficient amount, described histologic lesion is serious.

Especially, DNA in nucleus may be influenceed by being related to the free radical tissue damage of cell tissue nucleic acid.Therefore, This long-term be exposed to may cause the cell effect for causing cancer to occur in pollutant.

Particulate Air pollutant, especially gas-kinetic diameter are less than 10 μm of particle, due to its response to oxidative stress A large amount of generations and free radical generation, may be to the healthy particularly hazardous of the mankind.Without examining, this kind of Particulate Air pollutant Significant bodily tissue injury may be also resulted in, locally and systemically inflammation, cancer cell ultimately generate, and other induction inflammation Relevant disease.

In some geographic areas, air pollution has become the subject matter of city people's health.Especially, different sizes and The air pollution composition granule of composition especially has notable work in the ability of injury human health in the ability for cause oxidative stress With.Ultramicro powder and other processes and tiny particular matter less than 0.1 μm may be inhaled into and in vivo by macrophage Absorbed with epithelial cell.It is this to absorb the tendency with very strong induction human body oxidative stress.

In addition, other air pollutants, such as polycyclic aromatic hydrocarbon hydrocarbon (PAH), equally substantially increase oxidation Stress be with the danger of final tissue damage.

Anti-oxidizing compounds such as Tea Polyphenols and Flavonoid substances have the ability for removing these free radicals, it is meant that they Eliminate the danger that these free radicals enter human cell.In this case, these nutrient for plants, Tea Polyphenols and flavonoids Material reduces infringement of the free radical to cell and tissue.

The neutralization of free radical has a variety of benefits to human body.The benefit includes reducing coronary heart disease, cranial vascular disease, cancer With other tumour syndromes, diabetes, obesity, nerve degenerative diseases, arthritis, pollution and cigarette related tissue damage and add The risk of speed tissue aging.

Another common factors for improving interior free yl concentration is muscular training, the especially aerobic exercise of high intensity. Increased Skeletal Muscle Contraction produces a large amount of excessive free radical compounds.The generation of this free radicals a large amount of as caused by motion, Ability to act of the muscle in optimum level is hindered, extends the recovery period of overexercise, and muscle damage may be improved The danger of wound, it is referred to as pulled muscle or muscle tear.

The clinical research for performing high intensity interval tranining (HIIT) sportsman is shown, when sportsman take it is oral anti-oxidant During agent, its energy production and athletic performance are optimal.It has been shown that HIIT its there is the bone that suppression is referred to as human body " energy plants " The ability of bone Muscle Mitochondria, to eliminate the accumulation of the free radical related to moving.

The content of the invention

Technical problem solved by the invention is to lack in the prior art while have anti-oxidant with antiinflammatory action and in people The high dietary supplements of internal bioavailability.In order to solve the technical problem, the invention provides a kind of dietary supplements And its preparation method and application.

On the one hand, the invention provides a kind of dietary supplements, count in parts by weight, the dietary supplements includes：65- 85 parts of Moringa extracts, 5-12 parts hydroxytyrosol, 5-12 parts oleuropein and 5-11 part piperings.

Foregoing dietary supplements, is counted in parts by weight, and the dietary supplements includes：70-80 part Moringas extract, 7- 10 parts of hydroxytyrosols, 7-10 parts oleuropein and 6-10 part piperings.

Foregoing dietary supplements, is counted in parts by weight, and the dietary supplements includes：75 parts of Moringa extracts, 8 parts of hydroxyls Base tyrosol, 8 parts of oleuropeins and 9 parts of piperings.

Foregoing dietary supplements, the Moringa extract extract from leaf of Moringa, Moringa seed and/or Moringa flower.

Foregoing dietary supplements, the hydroxytyrosol extract from olive fruits, olive water extract and/or olive oil.

Foregoing dietary supplements, the oleuropein extract from olive fruits, olive water extract and/or olive oil.

Foregoing dietary supplements, the dietary supplements further comprise that dietetic product is upper and/or can pharmaceutically connect The carrier and/or auxiliary material received.

Foregoing dietary supplements, the dietary supplements are made into peroral dosage form；Preferably, the peroral dosage form is scattered Agent, tablet, capsule, granule, oral liquid or pill.

On the other hand, the invention provides the preparation method of foregoing dietary supplements, the preparation method to include：According to Predetermined ratio prepares Moringa extract, hydroxytyrosol, oleuropein and pipering；And by Moringa extract, hydroxytyrosol, olive Olive hardship glycosides and pipering mixing.

Foregoing preparation method, the Moringa extract use supercritical CO₂Abstraction technique and/or moisture film extraction filtering skill Art is prepared.

Foregoing preparation method, the hydroxytyrosol are prepared using moisture film extraction filtering technique.

Foregoing preparation method, the oleuropein are prepared using moisture film extraction filtering technique.

Foregoing preparation method, the pipering use supercritical CO₂Abstraction technique is prepared.

On the other hand, the invention provides foregoing dietary supplements to prepare health products or medicine with antioxidation Application in product.

On the other hand, the invention provides foregoing dietary supplements to prepare health products or medicine with antiinflammatory action In application.

On the other hand, the invention provides foregoing dietary supplements prepare prevention and/or treat it is following any one Or the application in the health products or medicine of a variety of diseases or symptom：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery Hardening, cancer, Arterial Hypertention, diabetes, Health cost caused by hypercholesterolemia and similar relevant disease, cigarette, Oxidative stress caused by pollution and tissue damage, age-related body aging, overweight and fat, bacterium infection, nerve move back The loss and motion loss that row disease, skin symptom, ultraviolet trigger.

The beneficial effects of the invention are as follows：The present invention dietary supplements is anti-oxidant and antiphlogistic effects are notable, to it is a variety of with it is scorching The disease or symptom of disease or oxidative stress correlation have good prevention or therapeutic action.The dietary supplements of the present invention, which has, matches somebody with somebody Fang Xinying, cost be cheap, evident in efficacy, the advantages that having no toxic side effect, and is applicable to industrialized production.

Embodiment

In order to be fully understood by the purpose of the present invention, feature and effect, by following embodiments, the present invention is made detailed Describe in detail bright.For the process of the present invention in addition to the description below, remaining uses the conventional method or device of this area.

Moringa (Moringa oleifera)

Moringa is a kind of edible trees, is grown on dry torrid areas in the world, and apply more and more In nutriment.It contains abundant protein and various nutrient elements.In leaf protein quality it is high due to, it It has been widely used for treatment malnutrition and Other diseases.

Moringa is rich in antioxidant phenols, flavonoids and carotenoid compounds.Oxidation resistant plant chemical substance is improving It is more and more useful in terms of human health.Their purposes comes from antioxidant and has been demonstrated that free radical in human body can be reduced The fact that concentration.

The seed of Moringa is also valuable protein source, and it, which is rich in, methionine and cysteine.These amino acid It is highly beneficial to health.In human body, cysteine can promote the generation of natural glutathione, and glutathione is effective day Right antioxidant.Because Moringa has abundant anti-oxidizing compounds and its special protein amino acid content, and these Composition promotes the generation of natural glutathione antioxidant agent, and therefore, Moringa has the property to human health.

Free radical is compound, molecule and the atom for losing an outer-shell electron, thus gives their unstable properties. In order to become more stable, free radical may obtain the electronics lost from the tissue of health and body fluid, therefore, this Property is harmful to human body.This phenomenon is referred to as " electronic theft " (electron stealing).So, it has been shown that This property of free radical may increase the risk of disease progression, including angiocardiopathy, cancer and other tumours, degeneration With diseases associated with inflammation, diabetes, obesity, nerve degenerative diseases.

Except its oxidation resistance, Moringa has been asserted the effect with anti-inflammatory.Alhakmani et al. is studied in vitro The effect of Moringa anti-inflammatory (Asian Pacific Journal of Tropical Biomedicine 2013；3(8)：623- 627).These antiphlogistic effects compare with commercially available Primary Reference anti-inflammatory agent C14H10Cl2NNaO2.They measure ovalbumin Thermal induction protein denaturation, and point out, the antiphlogistic effects of Moringa flower extract are similar to the antiphlogistic effects of C14H10Cl2NNaO2.

Identical research indicates the antioxidant content and feature of Moringa.Moringa flower contains 19.31mg/g nutgall Acid, equivalent to total phenol in dried extract.

Leaf of Moringa has also been proved to the serum levels that can reduce obese patient's LDL-C. (Journal of Ethnopharmacology.2000Jan in the research that Ghasi et al. is delivered；69(1)：21-5), tie Fruit shows that the total serum cholesterol in patients level for the high fat diet that feeding extracts from leaf of Moringa reduces 14.35%.This drop The influence of low serum cholesterol has statistical significance.It follows that Moringa leaf has clear and definite hypocholesterolemia Activity, it is beneficial to people on the line.

Hydroxytyrosol (Hydroxytyrosol)

The cardiovascular health benefits of Mediterranean diet, first by de Lorgeril (Circulation.1999；99：779- Et al. 785) it was described and delivered as scientific discovery in 1999, it was demonstrated that in the patient having a heart attack in the past, ground Middle extra large diet is to repeating heart attack with the protective effect for being up to 4 years (4 years are the long term follow-up phases most long in the research).

Similar research shows that all other hazards are identical, such as obesity, smoking, inactive and other diet Bad, because certain reason stays in, the regional people of Mediterranean basin are more healthy than in the other local adults in the world, and the life-span is more It is long.Mediterranean diet includes abundant fresh daily water fruits and vegetables, full cereal, nut, berry, olive, red wine, moderate The fish and poultry of intake, the dairy products of low intake, red meat, manufactured meat and candy, and particularly routine uses olive oil, Particularly Extra Virgin.

Further scientific research clearly illustrates that the main beneficial compound in Mediterranean diet is present in olive, special It is not in olive oil.Show after carrying out substantial amounts of comparative studies to the olive oil of different cultivars and grade, with original olive oil and Non-primary olive oil is compared, and higher quality and more refined Extra Virgin are more helpful to health.

Extra Virgin is different from common olive oil and virgin oil due to its excellent polyphenol content.Therefore Draw a conclusion, most of health benefits come from olive polyphenol in Mediterranean diet.

So far, in terms of anti-oxidant and anti-inflammatory power, maximally effective polyphenol is hydroxyl junket in olive fruit and olive oil Alcohol.Hydroxytyrosol is that olive oil has the reason for high stability.The parent compound of hydroxytyrosol is oleuropein, and its is main Found in olive leaf.The reason for it is the toil pungent of olive, and hydroxytyrosol is relatively tasteless.

The widest population studies for assessing Extra Virgin effect are always PREDIMED researchs (Estruch, et al.N Engl J Med2013；368：1279-1290).This is the large-scale Prevention Research carried out in Spain, is related to 7447 patients for suffering from high blood pressure disease.Research conclusion shows, supplements the Mediterranean of one liter of Extra Virgin weekly Diet significantly reduces the incidence of disease of angiocardiopathy.

Because monounsaturated fatty acids (MUFA) of the olive oil containing unsoundness, it is initially considered that these healthy greases rise Positive health discovery is come from, it is relevant with Mediterranean diet.However, when olive oil with other types of plant oil such as to day When certain herbaceous plants with big flowers oil and the safflower oil unsaturated oleic acid aliphatic acid of list of similar content (also contain) compare, other vegetable oil do not have generation and The positive health benefits of Mediterranean diet identical.

These find to confirm (Mol Nutr Food Res.2007Oct by P é rez-Jim é nez et al.；51(10)： 1199-208).It has recorded the anti-inflammatory of olive oil, anti-oxidant and less thrombosis effect can not contain due only to its MUFA Amount.

Therefore, Extra Virgin and other types of olive oil are analyzed in detail, analysis shows, it is and normal Rule are compared with virgin oil, and Extra Virgin contains more phenolic compounds (also referred to as polyphenols), special It is not more hydroxytyrosols, improves the higher level of health benefits.

The hydroxytyrosol and other polyphenolic substances found in olive and Extra Virgin is in medical science and section Learn and widely studied and delivered in the periodical literature of the peer review.They clearly show to have human health great Interests, it is both the basis of prevention, and the adminicle of patient of the treatment with specified disease.

Oleuropein (Oleuropein (OL))

Oleuropein (OL) is main bitter taste secoiridoid glycoside family phenolic compounds.Its derivative includes 2 kinds of masters The olive source alcohols wanted：1.3,4- dihydroxyphenyl ethanols, also referred to as oleuropein (HT), and 2. tyrosols, also referred to as Tyrosol (TY).

OL is present in the olive leaf without processing with the olive fruits before maturation, passes through chemistry after fruit maturation Method and enzyme process, which decompose OL, can produce HT and TY.

OL is hydrophilic compounds, therefore, the concentration in virgin oil (VOO) and Extra Virgin (EVOO) It is very low.Although the concentration of the OL and other phenols in EVVO are low, they are highly advantageous to health.

Oleuropein has a variety of health benefits having confirmed and medical application.Such as：

It is anti-oxidant.OL contains positively charged oxygen on its phenol carbocyclic ring, and OL chemical constitution makes it have removing free radical Ability and can be as protecting human body from the barrier of tissue damage and aging.

Antiinflammatory action.OL contributes to a variety of effects of anti-inflammatory.OL suppresses the biosynthesis of pro-inflammatory cytokine, especially It can reduce tumor necrosis factor α (TNF-α), interleukins-β (IL- β) and nitric oxide (NO).

Anti thrombotic action.The danger of arterial thrombus is reduced, so as to reduce the danger of heart disease and stroke.

Anti- athero- artery sclerosis effect.

Anticancer and blood vessel formation against function.Lyons cardiac studies have reported the angiocardiopathy that Mediterranean diet is brought first The beneficial effect reduced with cancer mortality.It has been reported that risk of cancer have dropped 61%.The main contributing factors of Mediterranean diet It is effective polyphenol in Extra Virgin.These phenolic compounds illustrate anticancer effect and anti-angiogenesis effect, borrow This, they reduce the ability that cancer cell points to human physiology approach again, and this ability of cancer cell can cause neovascularization, Referred to as angiogenesis.These effects are reconciled in phenol epigenetic regulation and/or the expression of the miRNA by changing.In addition, including olive Phenolic compounds including bitter glycosides can bring direct antitumor and pro-apoptosis bioactivity [Casaburi, et in a variety of cancerous cell lines al.Potential of olive oil phenols as chemopreventive and therapeutic agents against cancer：A review of in vitro studies.Mol.Nutr.Food Res.2013,57,71-83]. OL by endothelium suppression come prevent the effect of angiogenesis be likely to be by reduce VEGF (VEGF) and MMP-2, MMP-9 and MMP-13's expresses.

Anti-aging effects.Effects of the ROS to body tissue and cell can accelerate aging course.ROS is to anti-oxidant in body Unbalance generation aging [Halliwell B between substance protection effect：Oxidative stress and cancer：have we moved forwardBiochem.J 2007,401：1-11.]；[DurackováZ：Some current insights Into oxidative stress.Physiol.Res.2010,59：459-469].

Neuroprotection.By limiting mitochondrial DNA damage and oxidative stress, having proven to OL is helping to prevent A Er Important function has been played in the neuroprotection of Zi Haimo diseases and Parkinson's disease.OL suppresses the product of amyloplaste-β and Tao albumen.

By absorbing and contributing to delay senility process and then effective antioxidation of neutralization ROS materials, oleuropein.

The biological usability of height.It is anti-oxidant, which not reach sufficient amount in target tissue and organ, to be high as having been found The OL of degree bioavailable makes human body be benefited [Cicerale S, Lucas LJ, Keast RS like that：Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive Oil.Curr.Opin.Biotechnol.2012,23：129-135.]；[Cicerale S, Lucas L, Keast R： Biological activities of phenolic compounds present in virgin olive oil.Int.J Mol.Sci.2010,11：458-479.].

The main mechanism of OL effects includes：1) free radical scavenger and free radical chain fracture；2) resisting oxidation free radical；With 3) Metal-chelator.

OL catechol structure makes them remove peroxy radical and breaks peroxidating chain reaction to produce stabilization Resonant structure [Tripoli E, Giammanco M, Tabacchi G, DiMajo D, Giammanco S, La Guardia M： The phenolic compounds of olive oil：Structure, biological activity Andbeneficial effects on human health.Nutr Res Rev 2005,18：98-112.]；[Bulotta S, Oliverio M, Russo D, Procopio A：Biological Activity ofOleuropein and its Derivatives.In Natural Products.Edited by Ramawat KG, M é rillon JM.Berlin： Heidelberg Springer-Verlag；2013：3605-3638.].

Angiocardiopathy (CVD).OL antiphlogistic effects are known.CVD and chronic states body inflammation height correlation, Wherein body inflammation is caused by several factors, such as heredity, obesity, diabetes, smoking, exogenous pollution, diet, do not move and Other factorses.

OL can help to promote circulation by vasorelaxation action, reduce the influence of CVD results.It is anti-oxidant.Antiplatelet Aggregation.Thrombus in important blood vessels (such as the coronary artery of heart and arteria carotis and cerebral artery) causes poisonous and is typically to cause The cardiovascular event of life, such as miocardial infarction (heart disease) and apoplexy.OL resistant to aggregation effect helps to prevent such event.

Ldl cholesterol oxidation.LDL-C (LDL-C) is typically considered " bad " courage Sterol, because its internal arterial wall (endothelium) causes Atherosclerosis (blood platelet generation).However, work as LDL- After peroxidating, their effect is significantly enhanced C, and wherein LDL-C oxidation is adjusted in human body by copper sulphate abduction mechanism Section.Oleuropein slows down the circulatory diseases process by its scavenging action to HOCl, and HOCl is anti-in LDL-C lipid peroxidations Effect [Carr AC, Tijerina T, the Frei B of initiator is played during answering：Vitamin C protects against and reverses specifichypochlorous acid-and chloramine-dependent modifications Of low-density lipoprotein.Biochem.J 2000,346：491-499.].Thus, removings of the OL to HOCl is made With to lipoid peroxidization resistant and LDL-C oxidation can be reduced, the atherosclerosis disease of arterial endothelium is caused to deteriorate drop It is low, so as to reduce the risk of angiocardiopathy and cardiovascular event (such as miocardial infarction and apoplexy).

Pipering (Piperine)

Pipering (black pepper piper longum (piper longum) extract) is a kind of bioavilability reinforcing agent.Pipering One attracting feature is the inhibitory action that it is reacted enzyme drug biotransformation in liver.Pipering strong inhibition liver and intestines Road aryl hydrocarbon hydroxylase and UDP-glucoronosyl/transferase.

The Bioaugnentation effect of pipering is also attributed to the enhancing of intestinal absorption to a certain extent, and this is that pipering acts on The result of the ultra microstructure of microvillus on intestinal brush border.

Although current research indicates Moringa extract and hydroxytyrosol has anti-inflammatory and antioxidant properties, pipering is Bioavilability reinforcing agent, the present inventor by research it has unexpectedly been discovered that, when above-mentioned substance is according to special ratios Anti-inflammatory and antioxidant properties are significantly increased when being combined.

According to an aspect of the invention, there is provided a kind of dietary supplements, is counted in parts by weight, including：65-85 parts are peppery Wooden extract, 5-12 parts hydroxytyrosol, 5-12 parts oleuropein and 5-11 part piperings；Preferably, the dietary supplements bag Include：70-80 part Moringas extract, 7-10 parts hydroxytyrosol, 7-10 parts oleuropein and 6-10 part piperings；It is highly preferred that institute Stating dietary supplements includes：75 parts of Moringa extracts, 8 parts of hydroxytyrosols, 8 parts of oleuropeins and 9 parts of piperings.

The present inventor by research it has unexpectedly been discovered that, when Moringa extract, hydroxytyrosol, oleuropein and When pipering is combined according to the ratio of the present invention, is cooperated between three, there is synergy.Particularly, it is of the invention Combination of the dietary supplements based on foregoing four kinds of components can provide the prevention from suffering from the diseases of height to reduce or eliminate chronic body Inflammation and oxidative stress, and the antioxidant effect combined by them can reduce number of free radical and reactive oxygen species material (ROS)。

These four anti-oxidant and anti-inflammatory components act on the mankind and animal body to prevent specified disease by be combineding with each other, This ability is further enhanced by pipering, and the enteron aisle that pipering can take in nutrient for plants compound by increasing is inhaled Receive, and by reduce its in liver and intestinal wall inside be metabolized eliminate, and then increase its intake nutrient for plants compound Bioavilability.

On the other hand, when these four compositions are combined according to aforementioned proportion, by with Moringa extract, hydroxyl junket Alcohol, oleuropein bioactivity plant nutrient synergy, the positive health effect of pipering have also obtained greatly carrying High and amplification.These effects include：Anti-inflammatory, anti-oxidant, anti-rotation shifting, antibacterial, anti-mutation and antitumor and anti-apoptotic, these Effect is the pipering other properties healthy and beneficial to humans and animals, and in the presence of oleuropein and Folium Artemisiae Argyi extract recklessly These effects of green pepper alkali have obtained enhancing and further activation.

In addition to playing the function of bioperformance enhancing agent, by suppressing and being quenched free free radical and active oxygen thing Matter, and by reducing peroxidatic reaction of lipid and actively impact intracellular mercaptan state, antioxidant molecule and antioxidase, Pipering is also prevented from oxidative stress associated tissue damage.

Moringa extract, hydroxytyrosol, oleuropein and pipering are combined according to the ratio of the present invention, four collaborations Effect, compared with not using the replenishers that the present invention matches, the anti-oxidant and antiphlogistic effects of dietary supplements of the invention are not only It is significantly improved, and effect is lasting.With only taking appointing in Moringa extract, hydroxytyrosol, oleuropein and pipering Meaning is a kind of, compares for two or three, or, compared with not using the replenishers that the present invention matches, dietary supplements of the invention Antioxidant effect be significantly higher than the independent effect sum of three kinds of components.

It is a kind of systematically measure and compare food and the method for the oxidation resistance of replenishers be measurement oxygen radical absorb Ability (ORAC).The ORAC of hydroxytyrosol is 68,576 TE/ grams of micromoles, and the ORAC of oleuropein is 12,000 micromole TE/ Gram, the ORAC of Moringa extract is TE/ grams of 1576 micromole, and the ORAC of pipering is TE/ grams of 341 micromole, it is very small and because It is insignificant that this, which compares,.Therefore, the ORAC of dietary supplements of the invention is occupied an leading position by hydroxytyrosol, so And, it is contemplated that the bio-enhancer effect of pipering, total synergy of dietary supplements of the invention are more independent than each component ORAC sums will greatly at least 50%.

In the dietary supplements of the present invention, the main component of the Moringa extract be oxidant Polyphenols, flavonoids and Carotenoid compounds etc., it can extract from leaf of Moringa, Moringa seed and/or Moringa flower, it is preferred to use supercritical CO₂Extraction Technology and/or moisture film is taken to extract filtering technique and extracted.Both extractive techniques avoid having used ethanol, methanol or other What chemical reagent.

Moisture film extraction filtering technique only uses water as solvent, and supercritical CO₂Abstraction technique is (for example, using high pressure The supercritical CO of (100-150 bars) and low temperature (40 degrees Celsius)₂Abstraction technique) under elevated pressure conditions only using being perfectly safe Carbon dioxide.Moisture film extracts filtering technique while avoiding using chemical solvent, Moringa extract has been reached total phenol generalization Compound (polyphenol) and flavonoids (other bioactivity beneficial compounds) have highest concentration.

The antioxidation activity for the Moringa extract being obtained by extraction by water than other solvents for example methanol, acetone, ethanol and The activity that chloroform extracts is high.In order to obtain optimal anti-inflammatory bioactivity, super face is preferred in the extract in Moringa flower Boundary CO₂Extraction process produces.Therefore, the present invention extracts the extract and supercritical CO of filtering technique using moisture film₂Abstraction technique The composition that extract is formed realizes that the phenols of highest level is anti-oxidant and anti-inflammatory bioactivity.

In a kind of preferred embodiment, the extraction of Moringa includes following workshop section：

1. the plant material collection stage

Before the extraction stage, first the Moringa plant of harvest and seed material are screened, are cleaned, sterilized and ground, To produce the biological active component of final products.

First by the leaf of plant and seed transport carries out cleaning to extraction factory and plant part sorts.In order that antioxygen It is maximum to change phenols, flavonoids, the yield of carotenoid Phytochemistry compound, it is necessary to make whole plant material select and Handling process systematization.This will need following device：

(1) it is used for the conveyer belt and system for collecting plant material

(2) disleave and vibratory sieve separation device, to screen a variety of plant positions

(3) washing and sorting line, for cleaning and screening vegetable matter

2. the plant material primary treatment stage

Moringa plant material must be mechanically and chemically handled to maximize the extraction of bioactive compound.

Mainly extracted using distilled water to avoid using organic solvent.Use isolation technics, particularly film skill Art, to carry out flooding and purifying process.These technologies include ultrafiltration, filter, dialysis and electrodialysis successively.

Water soluble active component is extracted in hot water of the temperature less than 80 DEG C, such as anti-oxidant phenolic aldehyde, flavonoids and class are recklessly Radish extract plant chemical compound.Using supercritical CO₂Abstraction technique extracts other hydrophobic substances, such as saturation and insatiable hunger The aliphatic acid of sum.

There is variable hydrophobicity and partial hydrophilicity from the bioactivity vegetalization material of Moringa extraction, therefore can be with Using supercritical CO₂Abstraction technique and water film filtering technology extract these materials.Using analysis extraction Characterization of The Products come closely Monitoring optimization extraction.The standard of Such analysis includes：Uniformity, heat endurance, humidity, fragrance, freezing point, electric conductivity, pH and Gelling ability.

Carefully for the formulation of concern final products to produce various pulvis, tablet, capsule and liquid extract, this is very Important.

In a word, the Moringa extraction equipment needed for above-mentioned technique is as follows：

(1) plant and seed grinding device, including industrial grinding mills and plant and seed shredding machine.

(2) supercritical fluid CO₂Extraction equipment, extracted for hydrophobic plant chemical compound.

(3) solvent extraction system, this is directed to use with for water-soluble substances or the extraction of the water or ethanol of non-water soluble substance Device.

(4) the thick system for carrying, collecting and storing for Moringa, by stainless cylinder of steel and copper pipe of reservoir group into.

(5) system in extraction process needed for bulk liquids transhipment, is made up of pump and pipeline.

3. separation and enriching stage

Crude extract (including water and alcoholic solution) must be refined by using the isolation technics of filtering and Distallation systm. Then, obtained fraction is further concentrated by evaporating, to obtain the bioactive substance of higher concentration.

Further improve the concentration of purified extract using membrane technology, the membrane technology is that nanofiltration, counter-infiltration, freezing are dense Contracting, it is evaporated in vacuo.

The equipment in these stages includes：Membrane filtration system, for collecting the stainless cylinder of steel and reservoir system of liquid among liquid, For transporting the pump and pipe-line system of liquid, concentration and drainage equipment：Vacuum, fluid bed and thin film evaporator.

4. refining stage

Then, the liquid fraction of the concentration obtained to separation and concentration process further carries out refining operation, to incite somebody to action Specific compound (polyphenol and flavonoids) separates with other materials (salt, sugar and protein).This effectively increases bioactivity The yield and purity of compound.Further, it is necessary to convert liquids to stable formulation such as powder, and therefore need to dry system System.

Equipment：

(1) chromatographic system, it is equipped with the industrial columns of ion-exchange chromatography

(2) drying system：Fluidized bed drying system, spray drying system, freeze-drier

In the dietary supplements of the present invention, the hydroxytyrosol extracts from olive fruits, olive water extract and/or olive Oil, it is preferred to use moisture film technology (i.e. moisture film extraction filtering technique or water film filtering technology).Using water as unique solvent, keep away Exempt to use chemical substance, the antioxidation activity of maximum can be realized by water film filtering technology.Membrane filtration technique including the use of ultrafiltration, Filter, dialysis and electrodialysis.The concentration of purified extract can be carried out in the steps below using membrane technology：Nanofiltration, counter-infiltration, freezing Concentration and vacuum evaporation.

The present invention dietary supplements in, the oleuropein extract from olive fruits, olive leaf, olive plant chimney stalk, Olive water extract and/or olive oil, it is preferred to use moisture film technology (i.e. moisture film extraction filtering technique or water film filtering technology).Use Water avoids using chemical substance, the antioxidation activity of maximum can be realized by water film filtering technology as unique solvent.Film mistake Filter technology is including the use of ultrafiltration, filter, dialysis and electrodialysis.The concentration of purified extract can be entered in the steps below using membrane technology OK：Nanofiltration, counter-infiltration, freeze concentration and vacuum evaporation.

There are many methods to be used for extracting pipering from black pepper.It should be noted that start extraction process it Before, it is necessary to first black pepper is ground using industrial grinding mills.

Pipering can be extracted using dichloromethane.Using aqueous cosolvent, it can bring high yield and high selectivity. The conventional method of another kind be using potassium hydroxide solution solvent-free residue of the processing from black pepper ethanol extract so as to Remove the insoluble residue washed in resin and warm ethanol.Alkaloid is crystallized during this by cooling.This is directed to use with Water and ethanol extractor for water-soluble substances.

Discounting for cost issues, supercritical CO is preferably used under elevated pressure conditions₂Abstraction technique come extract recklessly Green pepper alkali, because this method is only using harmless and overall safety carbon dioxide and to bring high-purity, high yield under high pressure The pipering of amount.

In addition to the above-mentioned content referred to, the other specific process parameters and condition that are related in the above method are this area skills Art personnel can determine that the appropriate adjustment of process conditions will not be to extract in meals of the invention according to needs of production Effect played in replenishers produces materially affect.

The dietary supplements of the present invention can be according to aforementioned proportion by Moringa extract, hydroxytyrosol, the oleuropein of extraction Formed with pipering using conventional method is well mixed.

The dietary supplements of the present invention can further comprise that dietetic product is upper and/or pharmaceutically acceptable carrier with/ Or auxiliary material, for example, filler, disintegrant, lubricant, suspending agent, adhesive, sweetener, flavouring, preservative etc..Filler Including：Starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose etc..Disintegrant includes：Carboxymethyl forms sediment Powder sodium, PVPP, low-substituted hydroxypropyl cellulose, Ac-Di-Sol etc..Lubricant includes：It is stearic Sour magnesium, lauryl sodium sulfate, talcum powder, silica etc..Suspending agent includes：Polyvinylpyrrolidone, microcrystalline cellulose, sugarcane Sugar, agar, hydroxypropyl methyl cellulose etc., adhesive includes, starch slurry, polyvinylpyrrolidone, hydroxypropyl methyl cellulose Deng.Sweetener includes：Saccharin sodium, aspartame, sucrose, honey element, enoxolone etc..Flavouring includes：Sweetener and various Essence, preservative include：Parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, chlorhexidine acetate, Eucalyptus oil etc..By adding corresponding carrier and/or auxiliary material, oral agents can be further made in dietary supplements of the invention Type, for example, powder, tablet, capsule, granule, oral liquid or pill etc..In actual production, those skilled in the art can The dosage of carrier and auxiliary material is determined according to being actually needed, to reach optimal using effect.

When preparing final dietary supplement product, it is necessary to which the production system of such as tablet and capsule, what is be related to is specific Equipment is to prepare capsule and the powder compressibility needed for tablet.In addition, when preparing final dietary supplement product, also relate to And packing stage, i.e., final products are packed with solid dosage forms or liquid dosage form, be primarily related to be used for encapsulating products Blister packaging equipment and bottling line for fluid product.

According to another aspect of the present invention, it is turned into the invention provides foregoing dietary supplements in preparation with antioxygen Application in health products or medicine.

According to another aspect of the present invention, there is antiinflammatory action in preparation the invention provides foregoing dietary supplements Health products or medicine in application.

According to another aspect of the present invention, the invention provides foregoing dietary supplements to prepare prevention and/or control Treat the application in the health products or medicine of any one or more following disease：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery sclerosis, cancer, Arterial Hypertention, diabetes, caused by hypercholesterolemia and similar relevant disease, cigarette Oxidative stress and tissue damage, age-related body aging, overweight and fat, bacterium sense caused by Health cost, pollution The loss and motion loss that dye, nerve degenerative diseases, skin symptom, ultraviolet trigger.

Specifically, dietary supplements of the invention has significant prevention and/or therapeutic effect to following disease or symptom：

1. angiocardiopathy, for example, apoplexy, myocardial infarction and artery sclerosis.The dietary supplements of the present invention passes through reduction Inflammation reduces the risk of angiocardiopathy with anti-oxidant feature is improved, and can reduce the ability that blood forms thrombus grumeleuse, So as to reduce the ability of their blocked coronary arteries and arteria carotis inner chamber.

2. cancer, such as breast cancer, colon cancer, prostate cancer, cancer of pancreas, lung cancer, and some other tumours.This hair Bright dietary supplements can suppress growth of cancer cells or directly kill cancer cell, and effectively suppress cancer blood circulation.In addition, this The chemical signal of generation blood vessel of the dietary supplements of invention to being sent by cancer cell, which has, directly suppresses anti-angiogenesis work With so as to which the survival rate for the cancer cell for causing to contact with dietary supplements of the invention reduces.

The research of inventor also has confirmed another mechanism of pre- anti-cancer, and it is based on passing through CNRl promoters site The change of DNA methylation carrys out transient state up-regulation CNRl tumor suppressor genes.

3. diabetes.The research of inventor shows, periodically takes the diabetic of the dietary supplements of the present invention, display Its fasting blood glucose level and the horizontal reduction of HbAlc (HbAlc).These results show the dietary supplements of the present invention to sugar Urine patient has positive effect.

Wherein, HbAlc is the subgroup for circulating red blood cell (red blood cell), and in diabetes and pre-diabetic, it contains Amount is higher than normal range (NR) (4-5.6%), and this is attributed to the corpuscular hemoglobin irreversible glycosylation in β chain N- ends.Scope is 5.7-6.4% HbAlc high value is consistent with pre-diabetes condition, so as to add the risk for suffering from diabetes.6.5% or Higher level is consistent with the diagnosis to diabetes.The dietary supplements of the present invention can reduce diabetes and prediabetes is suffered from The HbAlc of person is horizontal.

4. cholesterol, particularly serum cholesterol.The dietary supplements of the present invention is to reducing serum low-density LP (LDL-C) has remarkable effect.

In addition, the dietary supplements of the present invention can cause the aobvious of the serum-concentration of OxLDL ELISA (ox-LDL) Work reduction and referred to as the inflammation blood plasma biomarker of plasma C-reactive protein (CRP) significantly reduce.LDL-C pairs of oxidation The harm of cardiovascular health is bigger, because the LDL-C of oxidation produces artery, (particularly heart and arteria carotis is coronal dynamic Arteries and veins) fat lesion atherosclerotic plaque ability it is stronger.

In addition, the dietary supplements of the present invention can increase the serum levels of HDL (HDL cholesterol), this It is beneficial in terms of reducing the health risk for suffering from artery sclerosis.The research of inventor also shows that the dietary supplements of the present invention improves HDL cholesterol efflux abilities, this can improve the healthy curve of user by improving the physiological function of body, so as to clear The artery that reason has been influenceed by atherosclerosis.

5. hypertension.Research in laboratory scope shows that dietary supplements of the invention significantly reduces systolic pressure And diastolic pressure.

Inventor also found that dietary supplements of the invention is significant to reduce blood pressure and improve with slight high by research The endothelial function of the young woman of blood pressure.Due to, it is known that the lifting in hyperpietic is treated of the function of arterial wall endothelial cell Act on, therefore endothelial function is extremely important in the elevated patient of blood pressure.Inner skin cell function is better, and blood pressure is more normal.

6. extend the life-span.The zoopery that inventor is carried out shows, periodically takes in the dietary supplements of the present invention daily The life-span of the control group adult mice of dietary supplements of the life-span of adult mice than not taking in the present invention is extended.Herein In the positive health effect expection enumerated the effect in similar extension life-span can be produced to people.

The research of inventor is it is also shown that the dietary supplements of the present invention can slow down cell ageing by some physiological phenomenons Process.The dietary supplements of the present invention can energetically resist epigenetics (such as DNA methylation) change, telomere is cut down. The dietary supplements of the present invention can also prevent mitochondria dysfunction and stem cell is exhausted and cell ageing.The meals of the present invention Food replenishers can prevent the oxidative damage to inhereditary material, so as to promote genome stable.

7. smoking.It is well known that one of harmful mechanism of harmfulness of smoking health is drawn with main and secondary smoking The body inflammation-related risen.This inflammation causes various disease-angiocardiopathies and cancer to be most common two kinds.

Daily ingestion of dietary supplements of the invention helps to protect human body from harm of smoking.Its mechanism of action is： 1) prevents oxidative stress caused by smoke from cigarette, 2) prevents the damage of lung's antioxidase and increased lipid caused by nicotine Peroxidation, 3) prevents the inflammation damnification directly contacted between smoke from cigarette and bronchial wall tissue, 4) prevents nicotine Angiogenesispromoting effect, suppress the growth of tumour and cancer.

8. air pollution.Similar to smoke from cigarette, air pollution can cause oxidative stress, reduce body antioxidase, and And it is proinflammatory disease.The dietary supplements of the present invention can protect the body from free radical and aoxidize to answer because of its anti-oxidation characteristics Swash.This protection is then also possible to prevent the sequelae of oxidative stress and air pollution, such as COPD and cancer.

9. skin protection.Skin health is harmful to exposed to ultraviolet radiation.Ultraviolet can damage the bottom branch of skin Frame albumen (being referred to as collagen and elastin laminin) suppresses skin corium fibroblast metabolism and produces collagen and elastic egg In vain, and generation also causes dermal tissue insult free radical.

The dietary supplements of the present invention helps to protect skin by eliminating skin free radical, so as to limit the group of correlation Knit damage.In addition, the dietary supplements of the present invention also enhances the generation of glutathione, this also contributes to protect the skin from certainly By the injury of base.

The dietary supplements of the present invention has direct inhibitory action to cancer cell, so as to contribute to the skin of protection exposure From the cutaneum carcinoma of UV inductions, such as the invasion and attack of melanoma.

10. arthritis.Arthritis be it is a kind of influence body joints inflammatory disease, including backbone, buttocks, knee, shoulder, Ancon, ankle, wrist, pin and pin.Soft tissue inflammation and cartilage inflammation of the dietary supplements of the present invention to arthritis and correlation With direct effect.

11. body weight.The present inventor is by studying discovery, the increasing that dietary supplements of the invention can lose weight Add, so as to effectively prevent and treat obesity.

12. skin whitening.The dietary supplements of the present invention can directly reduce melanocyte activity and increase gluathione The generation in vivo of peptide, so as to be advantageous to skin whitening.

13. mental health.The dietary supplements of the present invention is by promoting elimination intracerebral amyloid-beta to provide god Through protection, so as to the prevention and treatment for nerve degenerative diseases such as alzheimer disease (AD) etc..

14. infection.Experiment in vitro proves that dietary supplements of the invention has the property of antibacterial, therefore the meals of the present invention Eating replenishers can be as the antiseptic applied to prevention enteron aisle and respiratory tract infection.

15. gene clock (Gene Clock).Bioactive substance can in some levels (transcription, RNA it is ripe and steady Determine, translate into protein and posttranslational modification) influence gene expression.Transcript profile occurs on individual cell level and and organism Distinctive genome is completely different.According to the research of inventor, have been reported that now clock Physiological effect gene (CLOCK, Circadian Locomotor Output Cycles Kaput) ratios that methylate of CLOCK CpGs 1 and 8 show, are single Unrighted acid (MUFA) (Milagro, F.I.et relevant with the intake of aldehydes matter with polyunsaturated fatty acid (PUFA) Al.Clock, PER2 and BMAL1 DNA methylation：Association with obesity and metabolic syndrome characteristics and monounsaturated fat Intake.Chronobiol.Int.2012,29,1180-1194.).Moringa is rich in foregoing phenolic material.The phenols group of the present invention Divide has positive role for up-regulation Clock and BMAL1 gene activities.CLOCK genes are substantially responsible for biological clock section Rule system, the system instruct 24- circadian rhythm 24s on gene level.The gene is related to fat and metabolic syndrome.Study people Member is it has been found that the patient with visceratonia abdominal fat obesity and the patient with metabolic syndrome show circadian rhythm disorders Symptom (timer interrupt), the illness can produce larger increased weight and then fat tendency, further develop meeting Cause diabetes and atherosclerosis (G ó mez-Santos, C.et al.Circadian rhythm of clock genes In human adipose explants.Obesity 2009,17,1481-1485).The dietary supplements of the present invention can be adjusted CLOCK gene activation approach is controlled, so as to help to prevent and reduce to cause the factor in terms of the gene of obesity and metabolic syndrome.

Causality (Yoshino J, Klein S.A is also likely to be present between the suppression of CLOCK genes and diabetes B Novel Link Between Circadian Clocks and Adipose Tissue Energy Metabolism.Diabetes Jul 2009.62(7)：2175-7).Therefore, the regulation and control to CLOCK genes contribute to prevention or Reduce the seriousness of diabetes B.

The research of inventor displays that the epigenetic variation of CLOCK genes is relevant with breast cancer incidence raising, and this is Because the reduction that methylates of the CLOCK promoter regions of the women to suffer from breast cancer.The dietary supplements of the present invention being capable of actively impact These are mutated and reduce their influences to CLOCK genes, so as to reduce the risk to suffer from breast cancer.

The research of inventor displays that, causes have height between the somatic mutation of colorectal cancer and CLOCK mutation Correlation.The dietary supplements of the present invention helps to stablize CLOCK genes to prevent CLOCK mutation, pre- so as to contribute to CLOCK is mutated in the anti-microsatellite instability target gene for causing colorectal cancer.

Effect of the dietary supplements of the present invention on CLOCK gene expressions can also influence circadian rhythm period, thus regulate and control Sleep pattern.CLOCK and CYCLE albumen (circulating protein) (dBMAL genes) can form dimer, the compound finally with Period (gene of per, Per 1) and timeless (tim genes) promoter combine so as to raise (stimulations) per genes with Tim genes.Therefore, dietary supplements of the invention is the adjusting control agent of CLOCK genes.The regulation activity, which can treat and prevent, sleeps Dormancy rhythm disorder, and can treat and prevent in the associated disorders caused by the travelling of multiple time zones, the i.e. time difference.

16. take exercise and move.Free radical (FR), also referred to as active oxygen and nitrogen material (RONS), one as eubolism Part constantly produces (Bentley, et al.Acute and Chronic Effects of Antioxidant in human body Supplementation on Exercise Performance.Lamprecht M, editor.Antioxidants in Sport Nutrition.Boca Raton(FL)：CRC Press/Taylor&Francis；2015)；(Finaud J, Lac G, Filaire E.Oxidative stress.Sports Medicine.2006；36：327-358).

The FR/RONS of high concentration is harmful to human body, can be caused to the cyto-architectural tight of lipid, protein and DNA Re-oxidation destroys (Halliwell B, Gutteridge J MC.Free Radicals in Biology and Medicine.Oxford：Oxford University Press；1999).

Admittedly, daily exercise and high intensity physical training, including high intensity interval tranining (HIIT), can cause it is a large amount of from By the generation of base.Balance between obtainable antioxidant and oxidant, which has confirmed, can influence contraction of muscle, muscle strength Produce and energy exports (Clarkson P.M, Thompson H.S.Antioxidants：What role do they play in physical activity and healthThe American Journal of Clinical Nutrition.2000；72：637s-646s.).

To the clinical research confirmation that the sportsman for carrying out high intensity interval tranining (HIIT) exercise is carried out when sportsman takes Ability during oral antioxidant produces and exercise behaves oneself best (Lafay S, Jan C, Nardon K.et al.Grape extract improves antioxidant status and physical performance in elite male athletes.Journal of Sports Science and Medicine.2009；8：468-480.)；(Shafat A, Butler P, Jensen R.L, Donnelly A.E.Effects of dietary supplementation with vitamins C and E on muscle function during and after eccentric contractions in humans.European Journal of Applied Physiology.2004；93：196-202.).

HIIT has shown that the ability for the skeletal muscle mitochondrial for suppressing to be referred to as human body " energy plants " to remove and to take exercise Accumulation caused by relevant free radical.

The dietary supplements of the present invention makes muscle performance and strength and energy export and maximize, by the oxygen of musculature The property changed damage is reduced and minimized it, also, maximizes energy production during intake before exercise.By avoiding mitochondria Damage and FR/RONS outputs reduce, and dietary supplements of the invention can also strengthen muscle recovery after exercise.

Embodiment

The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to described reality Apply among a scope.The experimental method of unreceipted actual conditions in the following example, conventionally and condition, or according to business Product specification selects.

Embodiment 1

The composition of the dietary supplements of embodiment 1 be 75mg Moringas extract, 8mg hydroxytyrosols, 8mg oleuropeins and 9mg piperings.

Preparation method：

75mg Moringas extract, 8mg hydroxytyrosols, 8mg oleuropeins and 9mg piperings are well mixed, produce implementation The dietary supplements of example 1.

Embodiment 2

The composition of the dietary supplements of embodiment 2 be 65mg Moringas extract, 12mg hydroxytyrosols, 12mg oleuropeins and 11mg piperings.

Preparation method：

65mg Moringas extract, 12mg hydroxytyrosols, 12mg oleuropeins and 11mg piperings are well mixed, produce reality Apply the dietary supplements of example 2.

Embodiment 3

The composition of the dietary supplements of embodiment 3 be 85mg Moringas extract, 5mg hydroxytyrosols, 5mg oleuropeins and 5mg piperings.

Preparation method：

85mg Moringas extract, 5mg hydroxytyrosols, 5mg oleuropeins and 5mg piperings are well mixed, produce implementation The dietary supplements of example 3.

Embodiment 4

The composition of the dietary supplements of embodiment 4 be 70mg Moringas extract, 10mg hydroxytyrosols, 10mg oleuropeins and 10mg piperings.

Preparation method：

70mg Moringas extract, 10mg hydroxytyrosols, 10mg oleuropeins and 10mg piperings are well mixed, produce reality Apply the dietary supplements of example 4.

Embodiment 5

The composition of the dietary supplements of embodiment 5 be 80mg Moringas extract, 7mg hydroxytyrosols, 7mg oleuropeins and 6mg piperings.

Preparation method：

80mg Moringas extract, 7mg hydroxytyrosols, 7mg oleuropeins and 6mg piperings are well mixed, produce implementation The dietary supplements of example 5.

Embodiment 6

The composition of the dietary supplements of embodiment 6 be 82mg Moringas extract, 6mg hydroxytyrosols, 6mg oleuropeins and 6mg piperings.

Preparation method：

82mg Moringas extract, 6mg hydroxytyrosols, 6mg oleuropeins and 6mg piperings are well mixed, produce implementation The dietary supplements of example 6.

Embodiment 7：Oral liquid

The dietary supplement product of embodiment 7 is oral liquid, and the capacity of every oral liquid is 15ml, including：85mg Moringas Extract, 5mg hydroxytyrosols, 5mg oleuropeins, 5mg piperings, sucrose 20mg and water 15ml.

Preparation method：By 85mg Moringas extract, 5mg hydroxytyrosols, 5mg oleuropeins, 5mg piperings, sucrose 20mg It is dissolved in 15ml water, is well mixed；Homogenization is then carried out in homogenizer, various nutritional ingredients is homogenized, makes product Stability further ensured；Then carry out filling, produce the oral liquid of embodiment 7.

Embodiment 8：Oral liquid

The dietary supplement product of embodiment 8 is oral liquid, and the capacity of every oral liquid is 10ml, including：70mg Moringas Extract, 10mg hydroxytyrosols, 10mg oleuropeins, 10mg piperings, sucrose 20mg and water 10ml.

Preparation method：By 70mg Moringas extract, 10mg hydroxytyrosols, 10mg oleuropeins, 10mg piperings, sucrose 20mg is dissolved in 10ml water, is well mixed；Homogenization is then carried out in homogenizer, various nutritional ingredients is homogenized, makes The stability of product is further ensured；Then carry out filling, produce the oral liquid of embodiment 8.

Embodiment 9：Oral liquid

The dietary supplement product of embodiment 9 is oral liquid, and the capacity of every oral liquid is 3ml, including：75mg Moringas carry Take thing, 8mg hydroxytyrosols, 8mg oleuropeins, 9mg piperings, sucrose 20mg and water 3ml.

Preparation method：By 75mg Moringas extract, 8mg hydroxytyrosols, 8mg oleuropeins, 9mg piperings, sucrose 20mg It is dissolved in 3ml water, is well mixed；Homogenization is then carried out in homogenizer, various nutritional ingredients is homogenized, makes product Stability is further ensured；Then carry out filling, produce the oral liquid of embodiment 9.

Above-described embodiment is the preferable embodiment of the present invention, but the embodiment of present aspect is not by above-described embodiment Limitation, other any Spirit Essences without departing from the present invention with made under principle replacement, modification, combine, change, simplification Deng should be equivalent substitute mode, be included within protection scope of the present invention.Protection scope of the present invention should be with right What claim was limited is defined.

Claims

1. a kind of dietary supplements, it is characterised in that count in parts by weight, the dietary supplements includes：65-85 part Moringas carry Take thing, 5-12 parts hydroxytyrosol, 5-12 parts oleuropein and 5-11 part piperings.

2. dietary supplements according to claim 1, it is characterised in that count in parts by weight, the dietary supplements bag Include：70-80 part Moringas extract, 7-10 parts hydroxytyrosol, 7-10 parts oleuropein and 6-10 part piperings.

3. dietary supplements according to claim 1 or 2, it is characterised in that count in parts by weight, the dietary supplements Including：75 parts of Moringa extracts, 8 parts of hydroxytyrosols, 8 parts of oleuropeins and 9 parts of piperings.

4. according to the dietary supplements described in claim any one of 1-3, it is characterised in that the Moringa extract extracts from peppery Wooden leaf, Moringa seed and/or Moringa flower.

5. according to the dietary supplements described in claim any one of 1-4, it is characterised in that the hydroxytyrosol extracts from olive Fruit, olive water extract and/or olive oil.

6. according to the dietary supplements described in claim any one of 1-5, it is characterised in that the oleuropein extracts from olive Fruit, olive water extract and/or olive oil.

7. according to the dietary supplements described in claim any one of 1-6, it is characterised in that the dietary supplements further wraps Include that dietetic product is upper and/or pharmaceutically acceptable carrier and/or auxiliary material.

8. according to the dietary supplements described in claim any one of 1-7, it is characterised in that the dietary supplements is made into mouth Oral dosage form；Preferably, the peroral dosage form is powder, tablet, capsule, granule, oral liquid or pill.

9. the preparation method of the dietary supplements described in claim any one of 1-8, it is characterised in that the preparation method bag Include：Prepare Moringa extract, hydroxytyrosol, oleuropein and pipering according to predetermined ratio；And by Moringa extract, hydroxyl Tyrosol, oleuropein and pipering mixing.

10. preparation method according to claim 9, it is characterised in that the Moringa extract uses supercritical CO₂Extraction Technology and/or moisture film extraction filtering technique are prepared.

11. the preparation method according to claim 9 or 10, it is characterised in that the hydroxytyrosol was extracted using moisture film Filter technology is prepared.

12. according to the preparation method described in claim any one of 9-11, it is characterised in that the oleuropein is extracted using moisture film Filtering technique is taken to be prepared.

13. according to the preparation method described in claim any one of 9-12, it is characterised in that the pipering uses supercritical CO₂ Abstraction technique is prepared.

14. the dietary supplements described in claim any one of 1-8 is in health products or medicine with antioxidation are prepared Application.

15. the dietary supplements described in claim any one of 1-8 is in health products or medicine with antiinflammatory action are prepared Using.

16. dietary supplements described in claim any one of 1-8 prepare prevention and/or treat it is following any one or more Application in the health products or medicine of disease or symptom：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery sclerosis, Cancer, Arterial Hypertention, diabetes, Health cost, pollution are drawn caused by hypercholesterolemia and similar relevant disease, cigarette The oxidative stress and tissue damage, age-related body aging, overweight and fat, bacterium infection, nervus retrogression disease risen The loss and motion loss that disease, skin symptom, ultraviolet trigger.