CN107468696A - A kind of dietary supplements and its preparation method and application - Google Patents

A kind of dietary supplements and its preparation method and application Download PDF

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CN107468696A
CN107468696A CN201710588224.2A CN201710588224A CN107468696A CN 107468696 A CN107468696 A CN 107468696A CN 201710588224 A CN201710588224 A CN 201710588224A CN 107468696 A CN107468696 A CN 107468696A
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dietary supplements
extract
saturated
liquor
parts
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迈克尔·托马斯·麦克纳马拉
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Sunshine Medical Operation Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/58—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin
    • A61K31/585—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin containing lactone rings, e.g. oxandrolone, bufalin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A23L33/11—Plant sterols or derivatives thereof, e.g. phytosterols
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/05—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/4525—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with oxygen as a ring hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention provides a kind of dietary supplements, count in parts by weight, the dietary supplements includes：40 53 parts of liquor-saturated solanone extracts, 20 27 parts of withaferin A extracts, 10 20 parts of hydroxytyrosols and 10 20 parts of piperings.The dietary supplements of the present invention can further comprise upper dietetic product and/or pharmaceutically acceptable carrier and/or auxiliary material so that peroral dosage form is made.The dietary supplements of the present invention has remarkable anti-inflammatory and antioxidant effect, can be further used for preparing health products and medicine.

Description

A kind of dietary supplements and its preparation method and application
Technical field
The invention belongs to medicines and health protection field, in particular it relates to a kind of dietary supplements and preparation method thereof and Using.
Background technology
Although huge effort has been made in terms of the quality of health care is improved to extend human longevity, to provide It is more prone to the medical scheme obtained, but the incidence of disease of many serious diseases still persistently raises.These diseases include：Cardiac muscle stalk Plug (also referred to as heart disease), apoplexy, cancer, diabetes, hypertension, arthritis, obesity, chronic obstructive lung (COPD), its Its disease relevant with smoking or air pollution and nerve degenerative diseases such as Alzheimer's disease and Parkinson's disease.
The cost and activity of medical research now are unprecedented surging.Such research activities has confirmed many serious diseases (and causing the principal disease of human death) can be prevented.Such prevention is based on several factors, is related to heredity, drink Food, habits and customs and environmental factor.
Inflammation and its relation with disease
Today, the common physiologic feature of many most common and serious disease was relevant with the pathology for being referred to as inflammation.Slowly Property body inflammation be one be used for a series of body chemistries reaction and phenomenon broad terms, its metabolic state to body There is significant impact with process.
Inflammation is beneficial to human body.For example, excessively receive solar ultraviolet irradiation, can cause erythrosis and tan severely with Occur.In this case, inflammation excessively receive as body solar ultraviolet irradiation immune response, advantageously reduce by Acute and chronic damage caused by skin.
Influenza virus infection can cause body temperature to raise, and be referred to as having a fever.Fever is the inflammatory mechanism to protect the health of body, It is intended to reduce survival ability and the survival of intrusive viruses.Therefore, such inflammation is beneficial to the health of the mankind, and sometimes It is vital.
When for example hot pan in the surface of skin contact to the heat of the mankind, inflammation also occurs.In this case, Inflammation makes body produce a series of immunology and Circulatory response, to repair because contact high temperature damages to caused by skin Evil.Therefore, in several cases, inflammation is helpful to the health of body.
However, the generation and exacerbation of inflammation and specified disease caused by chronic body illness have very big relation.Coronary heart disease, Cancer, hypertension, diabetes, metabolic syndrome, hypercholesterolemia, arthritis, obesity and Alzheimer disease are demonstrate,proved It is bright relevant with the chronic abnormal rise of inflammation in vivo.Specific biomarker, particularly C reactive protein (CRP) can be entered Row is measured to prove these inflammation.
Inflammation is also likely to be that calorie is intensive, the direct result of the excessive food of natural deletions fiber consumption, which increase The risk of increased weight.Such increased weight can produce the accumulation of stomach fat and interior fat.Unnecessary stomach fat and The increase of the yield of the inflammatory molecule that is referred to as cell factor of the interior fat with that can enter the circulatory system has very big pass System.So, they can cause the inflammatory response of body, so as to increase the risk of relevant disease.
A kind of most obvious inflammatory molecule is interleukin-6 (IL-6) caused by stomach fat.In portal vein High-caliber IL-6 is measured, the vein of wherein portal vein from consumption stomach fat receives blood.In addition, high-caliber IL-6 with The CRP of high blood level is directly related.The oxidative stress of inflammation-induced can also increase the presence of other detrimental cytokines, these Detrimental cytokines include transforming growth factor-β (TGF-β) and tumor necrosis factor-alpha (TNF-α).
Disease caused by a kind of common and inflammation that is known is atherosclerosis.Inflammatory cytokine compound can The integrality of the crucial vascular endothelial cell of body, such as the arteria carotis of the coronary artery of heart and neck can be changed.Subsequent Inflammatory response promotes abnormal accumulation of the adipocyte in arterial endothelial cell.Such case is referred to as atherosclerosis, such as Fruit, which is developed as one pleases, may result in blood vessel diameter and significantly reduces.
Fat aggregation in vascular wall is referred to as patch.In some cases, it is complete may to lose its for unstable patch Whole property, especially in height inflammatory conditions or experience oxidative stress, cause plaque rupture.This phenomenon and the spot from damage Block is relevant with the blood leakage of final thrombus, and wherein thrombus is clot.The formation of arterial thrombus be cause most heart and The reason for a variety of apoplexy.
Chronic body inflammation is relevant with fasting blood glucose level rise, while relevant with ferroheme A1c increase.It is overweight and have The obese individuals of excessive internal organ and stomach fat are nor coincidence, they also can be to the influence of the circulation insulin as caused by pancreas Resistance is produced, ultimately results in the long-term rise of blood sugar level.This is referred to as diabetes B.
Also easily increase suffers from the risk of malignant tumour to patient with chronic body inflammation.Some most common human carcinomas Disease, including lung cancer, colorectal cancer, breast cancer, liver cancer, stomach cancer and prostate cancer are easily sent out compared with other healthy individuals Life is with the chronic increased patient of body level of inflammation.
Individual body phase of the verified patient with stages alzheimer's disease and Parkinson's disease of researcher with being not suffering from these diseases Than having higher levels of level of inflammation.
Oxidative stress and its relation with disease
In human body, oxidation is the important physiology course of production energy.However, during production energy, oxygen spreads out Raw free radical is also produced.Sometimes the generation of such free radical is uncontrolled and the histocyte of the mankind may be made Into a series of injuries.
Under its simplest pattern, when the amount deficiency that excessive free radical or anti-oxidizing compounds be present, or two kinds of feelings Condition simultaneously in the presence of, the oxidative stress of appearance is potentially to the process of histologic lesion.Caused by unopposed free radical activity Infringement be classified as oxidative stress.
Under normal physiological conditions, all atoms have paired outer-shell electron, and therefore have to adjacent tissue There is obvious reactivity.Free radical is defined as, containing single not paired electronics, substantially increasing its tissue reactive. Under biotic environment, due to these free radicals or compound may not make any distinction between with neighbouring molecule and cell effect, because The tissue reactive of this this free radical is probably breakneck.
Free radical may produce known " electronic theft " (electron stealing) phenomenon, cause tissue oxidizing And destination organization molecule may be made to lose activity.If the concentration of free radical is sufficiently large, these reactions are likely to result in extensively Cellular damage.
According to anti-oxidizing compounds relative bioavailability is neutralized, the degree of tissue damage may relax or reduce.This Class antioxidant directly and radical reaction, and neutralizes their reactivity, and linked groups' toxicity with low degree.
Oxidative stress may cause oxide-containing chemical material excessive, be referred to as active oxygen (ROS).The drastically rise of ROS concentration There may be obvious cellular damage.
Oxidative stress may be produced by many reasons, including diet is bad, toxin, radiation, medicine and air pollution.The world Contain a large amount of pollutants in the surrounding air in upper many areas.There are some even free radicals in itself in these air pollutants, Such as nitrogen dioxide.The acute and chronic result for contacting these air pollutants is that oxidative stress occurs for lung, once these are dirty Dye thing, which is drawn through and into the circulatory system, will trigger systemic oxidative stress.
The lung selective oxidation that air pollution triggers stress can then induce larger range of inflammatory reaction, then release in vivo Put more free radicals.If lack enough antioxidants, it may occur that serious tissue damage.
Especially, DNA in nucleus may be influenceed by being related to the free radical tissue damage of cell tissue nucleic acid.So, Therefore the cell effect for causing cancer to occur may be caused by being chronically exposed in pollutant.
Particle diameter is less than 10 μm of particle in airborne particulates, especially aerodynamics, because its oxidative stress is anti- The a large amount of generations and the generation of free radical answered, may be to the healthy particularly hazardous of the mankind.
These airborne particulates can also cause serious tissue damage, and locally and systemically property inflammation, ultimately forms cancer Cell simultaneously triggers other inflammation related diseases.
In some areas, air pollution has become chief city's residents ' health problem.Particularly, different sizes and group Into air pollution particle in terms of the ability that they endanger human health particularly their induced oxidations stress ability side Face plays an important role.Ultrafine particle and other tiny materials less than 0.1 μm can be inhaled into and by internal macrophage Taken in epithelial cell.This intake easily induces human body oxidative stress.
In addition, oxidative stress can also be significantly greatly increased for other air pollutants such as polycyclic aromatic hydrocarbon (PAH) and final tissue damages The danger of wound.
Anti-oxidizing compounds such as anthocyanidin and Flavonoid substances have the ability for removing these free radicals, it is meant that they Eliminate the danger that these free radicals enter human cell.In this case, these nutrient for plants reduce free radical pair Cell and the infringement prevented.
The neutralization of free radical has a variety of benefits to human body.The benefit includes reducing coronary heart disease, cranial vascular disease, cancer With other tumour syndromes, diabetes, obesity, nerve degenerative diseases, arthritis and the risk for accelerating tissue aging.
Another common factors for improving interior free yl concentration is muscular training, the especially aerobic exercise of high intensity. Increased Skeletal Muscle Contraction produces a large amount of excessive free radical compounds.
The generation of this free radicals a large amount of as caused by motion, hinders ability to act of the muscle in optimum level, extends Recovery period of overexercise, and the danger of muscle damage may be improved, it is referred to as pulled muscle or muscle tear.
The clinical research for performing high intensity interval tranining (HIIT) sportsman is shown, when sportsman take it is oral anti-oxidant During agent, its energy production and athletic performance are optimal.It has been shown that HIIT its there is the bone that suppression is referred to as human body " energy plants " The ability of bone Muscle Mitochondria, to eliminate the accumulation of the free radical related to moving.
The content of the invention
Technical problem solved by the invention is to lack in the prior art while have excellent anti-inflammatory and an antioxidation Dietary supplements.
On the one hand, the invention provides a kind of dietary supplements, count in parts by weight, the dietary supplements includes：40- 53 parts of liquor-saturated solanone extracts, 20-27 part withaferin As extract, 10-20 parts hydroxytyrosol and 10-20 part piperings.
Foregoing dietary supplements, is counted in parts by weight, and the dietary supplements includes：The liquor-saturated solanone extract of 43-51 parts, 21-25 part withaferin As extract, 12-18 parts hydroxytyrosol and 12-18 part piperings.
Foregoing dietary supplements, is counted in parts by weight, the dietary supplements include 47 parts of liquor-saturated solanone extracts, 23 parts Withaferin A extract, 15 parts of hydroxytyrosols and 15 parts of piperings.
Foregoing dietary supplements, the liquor-saturated solanone extract and withaferin A extract extract from withania somnifera (Withania somnifera) root, and/or withania somnifera (Withania somnifera) leaf.
Foregoing dietary supplements, the hydroxytyrosol extract from olive fruits, olive oil and/or water extract.
Foregoing dietary supplements, the pipering extract from black pepper.
Foregoing dietary supplements, the dietary supplements further comprise that dietetic product is upper and/or can pharmaceutically connect The carrier and/or auxiliary material received.
Foregoing dietary supplements, the dietary supplements are made into peroral dosage form；Preferably, the peroral dosage form is scattered Agent, tablet, capsule, granule, oral liquid or pill.
On the other hand, the invention provides the preparation method of foregoing dietary supplements, the preparation method to include：According to Predetermined ratio prepares liquor-saturated solanone extract and withaferin A extract, hydroxytyrosol and pipering；And by liquor-saturated solanone extract and Withaferin A extract, hydroxytyrosol and pipering uniformly mix.
Foregoing preparation method, the liquor-saturated solanone extract and withaferin A extract use supercritical CO2Abstraction technique And/or moisture film extraction filtering technique, and/or be impregnated and saturated with being prepared.
Foregoing preparation method, the hydroxytyrosol are prepared using moisture film extraction filtering technique.
Foregoing preparation method, the pipering use supercritical CO2Abstraction technique is prepared.
On the other hand, the invention provides foregoing dietary supplements to prepare health products or medicine with antioxidation Application in product.
On the other hand, the invention provides foregoing dietary supplements to prepare health products or medicine with antiinflammatory action In application.
On the other hand, the invention provides foregoing dietary supplements prepare prevention and/or treat it is following any one Or the application in the health products or medicine of a variety of diseases or symptom：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery Hardening, cancer, Arterial Hypertention, diabetes, Health cost caused by hypercholesterolemia and similar relevant disease, cigarette, Oxidative stress caused by pollution and tissue damage, age-related body aging, overweight and fat, bacterium infection and sleep Related obstacle, with anxiety and stress be related symptom and situation, nerve degenerative diseases, skin symptom and ultraviolet initiation Loss.
The beneficial effects of the invention are as follows：The present invention dietary supplements is anti-oxidant and antiphlogistic effects are notable, to it is a variety of with it is scorching The disease or symptom of disease or oxidative stress correlation have good prevention or therapeutic action.The dietary supplements of the present invention, which has, matches somebody with somebody Fang Xinying, cost be cheap, evident in efficacy, the advantages that having no toxic side effect, and is applicable to industrialized production.
Embodiment
It is described below being presently contemplated for the best mode embodiment of the present invention.The description is not to be considered as limiting, It is only used for the purpose of one or more preferred embodiments of the description present invention.Protection scope of the present invention should refer to right It is required that to determine.
Liquor-saturated solanone (Withanone) and withaferin A (Withaferin A)
Withania somnifera (Withania somnifera) is a kind of popular thousands of years of herbal medicine, for producing traditional remedy Measure and family's medicine, mainly planted in India and its neighbouring country.It is important that withania somnifera is that Ayurveda (Ayurveda) uses Medicinal herbs.It is the evergreen shrubs found in India arid area, grows into about 1 meter of height.There is leaf the tree whole year, and is grown in It is fertile, be sufficiently humidified so as to in well-drained soil.
Ayurveda is initiated by medical system of the India with history in 5000, is often translated into " life science ". Scientists start to verify the effect of Ayurveda herbal medicine in clinical practice recently.
Withania somnifera has time-honored application in Ayurveda medical science, beneficial to Health and Living custom, including：
(1) immunity,
(2) ＆ memories are learnt,
(3) anti-inflammatory,
(4) assisting sleep, and
(5) stress eliminate.
Withania somnifera is referred to as the renovation agent of Ayurveda medical science.Widely using always for many years, therefore people are It is extensively study to health promotion, Health restoration and the value for continuing life.
One of most ancient purposes of withania somnifera be as Ayurveda medicinal herbs resist stress influence.Withania somnifera is claimed Former to adapt to, can tackle user stress as caused by body or mental fatigue.Known withania somnifera has antiinflammatory property, its Have been used for treatment of arthritis and infectious diseases.
The reactive compound of withania somnifera just becomes more fully apparent recently.Recent research in cell and animal model is Channel syndrome understands the multifunctional number of mechanisms perhaps of withania somnifera：Anti-inflammatory, anticancer (Widodo N, et al.Selective killing of cancer cells by leaf extract of Ashwagandha：identification of a tumor-inhibitory factor and the first molecular insights to its effect.Clin.Cancer Res.2007Apr 1；13(7)：2298-306), anti-diabetic, resisting stress, anti-oxidant, neural Protection and immunological regulation.(Aalinkeel R, et al. (201O) Genomic Analysis Highlights the Role of the JAK-STAT Signaling in the Antiproliferative Effeets of Dietary Flavonoid-‘Ashwagandha’in Prostate Cancer Cells.Evid.Based Complement Alternat.Med.2010；7：177-187；Shah N, et al.Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells：combinational approach for enhanced differentiation.Cancer Sci.2009；100：1740-1747；Mayola E, et al.Withaferin A induces apoptosis in human melanoma cells through generation of reactive oxygen species and down-regulation of Bcl-2.Apoptosis 2009；16：1014-1027).
Withania somnifera has antioxidant properties.A use of especially important instruction of withania somnifera is neuroprotection, leads to Cross and remove the free radical in brain to prevent tissue damage.
Free radical is compound, molecule and the atom for losing an outer-shell electron, thus gives their unstable properties. In order to become more stable, free radical may obtain the electronics lost from the tissue of health and body fluid, therefore, this Property is harmful to human body.This phenomenon is referred to as " electronic theft ".So, it has been shown that this property of free radical may Can increase the risk of disease progression, including angiocardiopathy, cancer and other tumours, degeneration and diseases associated with inflammation, diabetes, Fat, nerve degenerative diseases.
Because the liquor-saturated solanone and withaferin A in withania somnifera are antioxidants, therefore the mechanism for promoting health activity is to increase Add the generation of internal natural anti-oxidation enzyme, including superoxide dismutase, catalase and glutathione peroxidase (Bhattacharya SK, Satyan KS, Ghosal S.Antioxidant activity of glycowithanolides from Withania somnifera.Indian J Exp.Biol.1997Mar；35(3)：236-9).By withania somnifera As prescription medicine to treat the brain diseases of the elderly, and improve cognitive disorder.
The various water-based and alcohol extracting thing of withania somnifera has been used for treating bacterium infection (Owais M (1), Sharad KS, Shehbaz A, Saleemuddin M.Antibacterial efficacy of Withania somnifera (ashwagandha)an indigenous medicinal plant against experimental murine salmonellosis.Phytomedicine.2005；Mar；12(3)：229-35).
Having proven to withania somnifera can effectively treatment of arthritis (Begum VH, Sadique J.Long term as antiinflammatory effect of herbal drug Withania somnifera on adjuvant induced arthritis in rats.Indian J Exp.Biol.1988Nov；26(11)：877-82).
Withania somnifera leaf contains a kind of compound for being referred to as steroidal lactone, particularly liquor-saturated eggplant lactone (withanolide), its In liquor-saturated solanone and withaferin A be bioactive compound (Kulkarni SK, Dhir A (2008) Withania somnifera： an Indian ginseng.Prog.Neuropsychopharmacol.Biol.Psychiatry.2008；32：1093- 1105)。
Except its oxidation resistance, liquor-saturated solanone and withaferin A compound has also been asserted the effect with anti-inflammatory.They It is especially effective to cancer cell, it can all be used as prevention sex-health replenishers to be used to treat and cancer protection.
Hydroxytyrosol (Hydroxytyrosol)
The cardiovascular health benefits of Mediterranean diet, first by de Lorgeril (Circulation.1999；99：779- Et al. 785) it was described and delivered as scientific discovery in 1999, it was demonstrated that in the patient having a heart attack in the past, ground Middle extra large diet is to repeating heart attack with the protective effect for being up to 4 years (4 years are the long term follow-up phases most long in the research).
Similar research shows that all other hazards are identical, such as obesity, smoking, inactive and other diet Bad, because certain reason stays in, the regional people of Mediterranean basin are more healthy than in the other local adults in the world, and the life-span is more It is long.Mediterranean diet includes abundant fresh daily water fruits and vegetables, full cereal, nut, berry, olive, red wine, moderate The fish and poultry of intake, the dairy products of low intake, red meat, manufactured meat and candy, and particularly routine uses olive oil, Particularly Extra Virgin.
Further scientific research clearly illustrates that the main beneficial compound in Mediterranean diet is present in olive, special It is not in olive oil.Show after carrying out substantial amounts of comparative studies to the olive oil of different cultivars and grade, with original olive oil and Non-primary olive oil is compared, and higher quality and more refined Extra Virgin are more helpful to health.
Extra Virgin is different from common olive oil and virgin oil due to its excellent polyphenol content.Therefore Draw a conclusion, most of health benefits come from olive polyphenol in Mediterranean diet.
So far, in terms of anti-oxidant and anti-inflammatory power, maximally effective polyphenol is hydroxyl junket in olive fruit and olive oil Alcohol.Hydroxytyrosol is that olive oil has the reason for high stability.The parent compound of hydroxytyrosol is oleuropein, and its is main Found in olive leaf.The reason for it is the toil pungent of olive, and hydroxytyrosol is relatively tasteless.
The widest population studies for assessing Extra Virgin effect are always PREDIMED researchs (Estruch, et al.N Engl J Med2013；368：1279-1290).This is the large-scale Prevention Research carried out in Spain, is related to 7447 patients for suffering from high blood pressure disease.Research conclusion shows, supplements the Mediterranean of one liter of Extra Virgin weekly Diet significantly reduces the incidence of disease of angiocardiopathy.
Because monounsaturated fatty acids (MUFA) of the olive oil containing unsoundness, it is initially considered that these healthy greases rise Positive health discovery is come from, it is relevant with Mediterranean diet.However, when olive oil with other types of plant oil such as to day When certain herbaceous plants with big flowers oil and the safflower oil unsaturated oleic acid aliphatic acid of list of similar content (also contain) compare, other vegetable oil do not have generation and The positive health benefits of Mediterranean diet identical.
These find to confirm (Mol Nutr Food Res.2007Oct by P é rez-Jim é nez et al.；51(10)： 1199-208).It has recorded the anti-inflammatory of olive oil, anti-oxidant and less thrombosis effect can not contain due only to its MUFA Amount.
Therefore, Extra Virgin and other types of olive oil are analyzed in detail, analysis shows, it is and normal Rule are compared with virgin oil, and Extra Virgin contains more phenolic compounds (also referred to as polyphenols), special It is not more hydroxytyrosols, improves the higher level of health benefits.
The hydroxytyrosol and other polyphenolic substances found in olive and Extra Virgin is in medical science and section Learn and widely studied and delivered in the periodical literature of the peer review.They clearly show to have human health great Interests, it is both the basis of prevention, and the adminicle of patient of the treatment with specified disease.
Pipering (Piperine)
Pipering (black pepper piper longum (piper longum) extract) is a kind of bioavilability reinforcing agent.Pipering One attracting feature is the inhibitory action that it is reacted enzyme drug biotransformation in liver.Pipering strong inhibition liver and intestines Road aryl hydrocarbon hydroxylase and UDP-glucoronosyl/transferase.
The Bioaugnentation effect of pipering is also attributed to the enhancing of intestinal absorption to a certain extent, and this is that pipering acts on The result of the ultra microstructure of microvillus on intestinal brush border.
Although current research indicate liquor-saturated solanone extract and withaferin A extract and hydroxytyrosol have anti-inflammatory and Antioxidant properties, pipering be bioavilability reinforcing agent, still, the present inventor it has unexpectedly been discovered that, when above-mentioned Anti-inflammatory and antioxidant properties are significantly increased when material is combined according to special ratios.
According to an aspect of the invention, there is provided a kind of dietary supplements, is counted in parts by weight, including：40-53 parts are liquor-saturated Solanone extract, 20-27 part withaferin As extract, 10-20 parts hydroxytyrosol and 10-20 part piperings；Preferably, the meals Food replenishers include：The liquor-saturated solanone extract of 43-51 parts, 21-25 part withaferin As extract, 12-18 parts hydroxytyrosol and 12-18 Part pipering；It is highly preferred that the dietary supplements includes：47 parts of liquor-saturated solanone extracts, 23 parts of withaferin A extracts, 15 parts Hydroxytyrosol and 15 parts of piperings.
The result of study of inventor shows, when liquor-saturated solanone extract, withaferin A extract, hydroxytyrosol and pipering are pressed When being combined according to aforementioned proportion, synergy can be obtained.Particularly, dietary supplements of the invention is based on these four components Combination ability, there is provided the pre- anti-cancer and auxiliary treatment and angiocardiopathy of height and the prevention of Other diseases, with drop Low or the chronic body inflammation of elimination and oxidative stress, and it is internal to reduce by the anti-oxidation characteristics of each of which and combination Number of free radical and reactive oxygen species material (ROS).These four anti-oxidant and anti-inflammatory components act on the mankind by be combineding with each other And animal body is further enhanced with preventing the ability of specified disease by pipering, pipering can be sought by increasing intake plant Support the intestinal absorption of plain compound, and by reduce intake nutrient for plants compound in liver and intestinal wall inside be metabolized and disappear Remove, to increase the bioavilability of the nutrient for plants compound of its intake.
On the other hand, when being combined as defined herein to these four compositions, pipering and hydroxytyrosol, liquor-saturated eggplant Ketone and withaferin A bioactivity vegetalization material synergy, the positive healthy beneficial effect of pipering is by more times of amplifications. These effects include：Anti-inflammatory, anti-oxidant, anti-rotation shifting, antibacterial, anti-mutation and antitumor properties, and antitumaous effect and anti-apoptotic Effect, these effects are the pipering other properties healthy and beneficial to humans and animals, and have obtained enhancing and further activation.
In addition to playing the function of bioperformance enhancing agent, by suppressing and being quenched free free radical and active oxygen thing Matter, and by reducing peroxidatic reaction of lipid and actively impact intracellular mercaptan state, antioxidant molecule and antioxidase, Pipering is also prevented from the related histologic lesion of oxidative stress.
Compared with the other replenishers for being not belonging to the scope of the invention, the anticancer of dietary supplements of the invention, anti-inflammatory and anti- Oxidation effectiveness is significantly improved, while effect is more longlasting.With only taking liquor-saturated solanone extract, withaferin A extract, hydroxyl junket Alcohol is compared with any one in pipering, two or three, or, compared with not using the replenishers that the present invention matches, this Anticancer, anti-inflammatory and the antioxidant effect of the dietary supplements of invention are significantly higher than the independent effect sum of four kinds of components.A kind of system The method that ground measured and compared the oxidation resistance of food and replenishers is measurement oxygen radical absorbability (ORAC).Hydroxyl junket The ORAC of alcohol is TE/ grams of 68,576 micromole.The ORAC of withania somnifera (Withania somnifera) extract is 1505 micro- Mole TE/100 grams.The ORAC of pipering is 34,053 micromole TE/100g.It is expected that each group of total cooperative effect than the present invention The summation height at least 100-200% divided.
The main component of withania somnifera (Withania somnifera) extract is compound in liquor-saturated eggplant lactone steroidal, Liquor-saturated solanone and withaferin A.Withania somnifera extract can be from withania somnifera (Withania somnifera) leaf and withania somnifera (Withania somnifera) root obtains, it is preferable that using supercritical CO2Abstraction technique and/or moisture film extraction filtering technique And/or it is impregnated and saturated with extracting liquor-saturated solanone extract and withaferin A extract.Both extractive techniques avoid having used ethanol, Methanol or other any chemical reagents.
Moisture film extraction filtering technique only uses water as solvent, and supercritical CO2Abstraction technique only makes under elevated pressure conditions With carbon dioxide that is harmless and being perfectly safe.Moisture film extracts filtering technique while avoiding using chemical solvent, obtains most Total liquor-saturated eggplant lactone bioactivity beneficial compound of high concentration.Water-based liquor-saturated solanone extract and withaferin A extract it is anti-oxidant Activity extracted than methanol, acetone, ethanol and chloroform solvent respective extract it is active high.Extract is preferred overcritical CO2Extraction process produces, to obtain optimal anti-inflammatory bioactivity.Therefore, the present invention by moisture film or will be impregnated and saturated with Liquor-saturated solanone and the withaferin A extract and liquor-saturated solanone and withaferin A supercritical CO that technology obtains2Extract combination, to obtain Liquor-saturated eggplant lactone anticancer, the anti-oxidant and anti-inflammatory bioactivity of highest level.
In a kind of preferred embodiment, extraction withania somnifera (Withania somnifera) obtains liquor-saturated solanone The extraction process of extract and withaferin A extract includes following workshop section：
1. the plant material collection stage
Before the extraction stage, first withania somnifera (Withania somnifera) plant of harvest and root timber material are carried out Screen, clean, sterilize and grind, to produce the biological active component of final products.
The leaf of plant and root are transported into extraction factory first and carry out cleaning and plant part sorting.In order that liquor-saturated eggplant lactone The yield of compound is maximum, it is necessary to makes whole plant material selection and processing process system.This will need following device：
(1) it is used for the conveyer belt and system for collecting plant material,
(2) disleave and vibratory sieve separation device, to screen a variety of plant positions, and
(3) washing and sorting line, for cleaning and screening vegetable matter.
2. the plant material primary treatment stage
Withania somnifera plant material must be mechanically and chemically handled to make the extraction of bioactive compound most Bigization.
Mainly extracted using distilled water to avoid using organic solvent.Use isolation technics, particularly film skill Art, to carry out flooding and purifying process.These technologies include ultrafiltration, filter, dialysis and electrodialysis successively.
Water-soluble active ingredient is extracted in hot water of the temperature for 100 DEG C or less than 100 DEG C, such as liquor-saturated solanone and liquor-saturated eggplant element A Phytochemistry compounds.By at 70 DEG C -100 DEG C, at preferably 75 DEG C -90 DEG C, most preferably 80 DEG C -90 DEG C heating and 1 DEG C - 30 DEG C, preferably 10 DEG C -30 DEG C, more preferably 20 DEG C -30 DEG C, at most preferably 20 DEG C -25 DEG C cooling carry out the step.
Liquor-saturated solanone and withaferin A Phytochemistry compound can also pass through supercritical CO2Abstraction technique is extracted.
The liquor-saturated solanone of liquor-saturated eggplant lactone and withaferin A bioactivity vegetalization material that can be extracted from withania somnifera can lead to Membrane filtration extraction is crossed, therefore due to its water-wet behavior, can use and use aqueous medium and CO2Two kinds of technologies, i.e., by That is heated at 70 DEG C -100 DEG C is impregnated and saturated with, and supercritical CO2Abstraction technique.Using analysis extraction Characterization of The Products come closely Monitoring optimization extraction.The standard of Such analysis includes：Uniformity, heat endurance, humidity, fragrance, freezing point, electric conductivity, pH and Gelling ability.
Carefully for the formulation of concern final products to produce various pulvis, tablet, capsule and liquid extract, this is very Important.
In a word, the liquor-saturated solanone of withania somnifera and withaferin A extraction equipment needed for above-mentioned technique are as follows：
(1) plant and root grinding device, including industrial grinding mills and plant and root cutter；
(2) supercritical fluid CO2Extraction equipment, extracted for hydrophobic plant chemical compound；
(3) solvent extraction system, this is directed to use with for water-soluble substances or the extraction of the water or ethanol of non-water soluble substance Device；
(4) be used for the system that slightly puies forward, collect and store, by stainless cylinder of steel and copper pipe of reservoir group into；And
(5) system in extraction process needed for bulk liquids transhipment, is made up of pump and pipeline.
3. separation and enriching stage
Crude extract (including the aqueous solution and alcoholic solution) must be carried out by using the isolation technics of filtering and Distallation systm Refining.Then, obtained fraction is further concentrated by evaporating, to obtain the bioactive substance of higher concentration.
Further improve the concentration of purified extract using membrane technology, the membrane technology is that nanofiltration, counter-infiltration, freezing are dense Contracting and vacuum evaporation.
The equipment in these stages includes：Membrane filtration system, for collecting the stainless cylinder of steel and reservoir system of liquid among liquid, For transporting the pump and pipe-line system of liquid, and concentration and drainage equipment：Vacuum, fluid bed and thin film evaporator.
4. refining stage
Then, the liquid fraction of the concentration obtained to separation and concentration process further carries out refining operation, to incite somebody to action Specific compound (polyphenol and flavonoids) separates with other materials (salt, sugar and protein).This effectively increases bioactivity The yield and purity of compound.Further, it is necessary to convert liquids to stable formulation such as powder, and therefore need to dry system System.
Equipment：
(1) chromatographic system, it is equipped with the industrial columns of ion-exchange chromatography；With
(2) drying system：Fluidized bed drying system, spray drying system, freeze-drier
In the dietary supplements of the present invention, the hydroxytyrosol extracts from olive fruits, olive water extract and/or olive Oil, it is preferred to use moisture film technology (i.e. moisture film extraction filtering technique or water film filtering technology).Using water as unique solvent, keep away Exempt to use chemical substance, the antioxidation activity of maximum can be realized by water film filtering technology.Membrane filtration technique including the use of ultrafiltration, Filter, dialysis and electrodialysis.The concentration of purified extract can be carried out in the steps below using membrane technology：Nanofiltration, counter-infiltration, freezing Concentration and vacuum evaporation.
Pipering is extracted from black pepper using one of several method.Before extraction process is started, it is necessary to use industry Grinder is first ground to black pepper.
Pipering can be extracted using dichloromethane.Using aqueous cosolvent, it can bring high yield and high selectivity. The conventional method of another kind be using potassium hydroxide solution solvent-free residue of the processing from black pepper ethanol extract so as to Remove the insoluble residue washed in resin and warm ethanol.Alkaloid is crystallized during this by cooling.This is directed to use with Water and ethanol extractor for water-soluble substances.
Due to of a relatively high expense, the high efficiency method being often eliminated is supercritical CO2Extraction, is used only under high pressure Harmless and overall safety carbon dioxide.This can bring the pipering of high yield.
In addition to the above-mentioned process conditions referred to, those skilled in the art can determine other according to needs of production Part.
The dietary supplements of the present invention can be prepared by following steps：According to aforementioned proportion prepare liquor-saturated solanone extract, Withaferin A extract, hydroxytyrosol and pipering, and by liquor-saturated solanone extract, withaferin A extract, hydroxytyrosol and pepper Alkali uniformly mixes.
The dietary supplements of the present invention can further comprise that dietetic product is upper and/or pharmaceutically acceptable carrier with/ Or auxiliary material, for example, filler, disintegrant, lubricant, suspending agent, adhesive, sweetener, flavouring, preservative etc..Filler Including：Starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose etc..Disintegrant includes：Carboxymethyl forms sediment Powder sodium, PVPP, low-substituted hydroxypropyl cellulose, Ac-Di-Sol etc..Lubricant includes：It is stearic Sour magnesium, lauryl sodium sulfate, talcum powder, silica etc..Suspending agent includes：Polyvinylpyrrolidone, microcrystalline cellulose, sugarcane Sugar, agar, hydroxypropyl methyl cellulose etc., adhesive includes, starch slurry, polyvinylpyrrolidone, hydroxypropyl methyl cellulose Deng.Sweetener includes：Saccharin sodium, aspartame, sucrose, honey element, enoxolone etc..Flavouring includes：Sweetener and various Essence, preservative include：Parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, chlorhexidine acetate, Eucalyptus oil etc..By adding corresponding carrier and/or auxiliary material, oral agents can be further made in dietary supplements of the invention Type, for example, powder, tablet, capsule, granule, oral liquid or pill etc..In actual production, those skilled in the art can The dosage of carrier and auxiliary material is determined according to being actually needed, to reach optimal using effect.
When preparing final dietary supplement product, it is necessary to which the production system of such as tablet and capsule, what is be related to is specific Equipment is to prepare capsule and the powder compressibility needed for tablet.In addition, when preparing final dietary supplement product, also relate to And packing stage, i.e., final products are packed with solid dosage forms or liquid dosage form, be primarily related to be used for encapsulating products Blister packaging equipment and bottling line for fluid product.
According to another aspect of the present invention, it is turned into the invention provides foregoing dietary supplements in preparation with antioxygen Application in health products or medicine.
According to another aspect of the present invention, there is antiinflammatory action in preparation the invention provides foregoing dietary supplements Health products or medicine in application.
According to another aspect of the present invention, the invention provides foregoing dietary supplements to prepare prevention and/or control Treat the application in the health products or medicine of any one or more following disease：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery sclerosis, cancer, Arterial Hypertention, diabetes, caused by hypercholesterolemia and similar relevant disease, cigarette Oxidative stress and tissue damage, age-related body aging, overweight and fat, bacterium sense caused by Health cost, pollution Dye, the obstacle related to sleep, with anxiety and stress be related symptom and situation, nerve degenerative diseases, skin symptom and The loss that ultraviolet triggers.
Specifically, dietary supplements of the invention has significant prevention and/or therapeutic effect to following disease or symptom：
1. angiocardiopathy, for example, apoplexy, myocardial infarction and artery sclerosis.The dietary supplements of the present invention passes through reduction Inflammation reduces the risk of angiocardiopathy with anti-oxidant feature is improved, and can reduce the ability that blood forms thrombus grumeleuse, from And reduce the ability of their blocked coronary arteries and arteria carotis inner chamber.
2. cancer, such as breast cancer, colon cancer, prostate cancer, cancer of pancreas, lung cancer, and some other tumours.This hair Bright dietary supplements can suppress growth of cancer cells or directly kill cancer cell, and effectively suppress cancer blood circulation.In addition, this The chemical signal of generation blood vessel of the dietary supplements of invention to being sent by cancer cell, which has, directly suppresses anti-angiogenesis work With so as to which the survival rate for the cancer cell for causing to contact with dietary supplements of the invention reduces.
The research of inventor also has confirmed another mechanism of pre- anti-cancer, and it is based on passing through CNR1 promoters site The change of DNA methylation carrys out transient state up-regulation CNR1 tumor suppressor genes.
3. diabetes.According to the research of inventor, the diabetic for periodically taking the dietary supplements of the present invention, display Its fasting blood glucose level and the horizontal reduction of glycated hemoglobin (HbA1c).These results show the dietary supplements of the present invention to sugar Urine patient has positive effect.
HbA1c is the subgroup for circulating red blood cell (red blood cell), and in diabetes and pre-diabetic, its content is higher than Normal range (NR) (4-5.6%), this is attributed to the corpuscular hemoglobin irreversible glycosylation in β chain N- ends.Scope is 5.7-6.4% HbA1c high value it is consistent with pre-diabetes condition, so as to add the risk for suffering from diabetes.6.5% or higher water It is flat consistent with the diagnosis to diabetes.The dietary supplements of the present invention can reduce diabetes and pre-diabetic's HbA1c is horizontal.
4. cholesterol, particularly serum cholesterol.The dietary supplements of the present invention is to reducing serum low-density LP (LDL-C) has remarkable effect.
In addition, the dietary supplements of the present invention can cause the aobvious of the serum-concentration of OxLDL ELISA (ox-LDL) Work reduction and referred to as the inflammation blood plasma biomarker of plasma C-reactive protein (CRP) significantly reduce.LDL-C pairs of oxidation The harm of cardiovascular health is bigger, because the LDL-C of oxidation produces artery, (particularly heart and arteria carotis is coronal dynamic Arteries and veins) fat lesion atherosclerotic plaque ability it is stronger.
In addition, the dietary supplements of the present invention can increase the serum levels of HDL (HDL cholesterol), this It is beneficial in terms of reducing the health risk for suffering from artery sclerosis.The research of inventor also shows that the dietary supplements of the present invention improves HDL cholesterol efflux abilities, this can improve the healthy curve of user by improving the physiological function of body, so as to clear The artery that reason has been influenceed by atherosclerosis.
5. hypertension.According to the research of inventor, dietary supplements of the invention significantly reduces systolic pressure and diastolic pressure.
Inventor also found that dietary supplements of the invention is significant to reduce blood pressure and improve the year with mild hypertension The endothelial function of light women.Due to, it is known that the function of arterial wall endothelial cell plays an important role in hyperpietic is treated, because This endothelial function is extremely important in the elevated patient of blood pressure.Inner skin cell function is better, and blood pressure is more normal.
6. extend the life-span.Zoopery shows, periodically takes in the longevity of the adult mice of the dietary supplements of the present invention daily The life-span of the control group adult mice of dietary supplements of the life than not taking in the present invention is extended.The combination enumerated herein Positive health effect expection the effect in similar extension life-span can be produced to people.
The research of inventor is it is also shown that the dietary supplements of the present invention can slow down cell ageing by some physiological phenomenons Process.The dietary supplements of the present invention can energetically resist epigenetics (such as DNA methylation) change, telomere is cut down. The dietary supplements of the present invention can also prevent mitochondria dysfunction and stem cell is exhausted and cell ageing.The meals of the present invention Food replenishers can prevent the oxidative damage to inhereditary material, so as to promote genome stable.
7. smoking.It is well known that one of harmful mechanism of harmfulness of smoking health is drawn with main and secondary smoking The body inflammation-related risen.This inflammation causes various disease-angiocardiopathies and cancer to be most common two kinds.
Daily ingestion of dietary supplements of the invention helps to protect human body from harm of smoking.Its mechanism of action is： 1) prevents oxidative stress caused by smoke from cigarette, 2) prevents the damage of lung's antioxidase and increased lipid caused by nicotine Peroxidation, 3) prevents the inflammation damnification directly contacted between smoke from cigarette and bronchial wall tissue, 4) prevents nicotine Angiogenesispromoting effect, suppress the growth of tumour and cancer.
8. air pollution.Similar to smoke from cigarette, air pollution can cause oxidative stress, reduce body antioxidase, and And it is proinflammatory disease.The dietary supplements of the present invention can protect the body from free radical and aoxidize to answer because of its anti-oxidation characteristics Swash.This protection is then also possible to prevent the sequelae of oxidative stress and air pollution, such as COPD and cancer.
9. skin protection.Skin health is harmful to exposed to ultraviolet radiation.Ultraviolet can damage the bottom branch of skin Frame albumen (being referred to as collagen and elastin laminin) suppresses skin corium fibroblast metabolism and produces collagen and elastic egg In vain, and generation also causes dermal tissue insult free radical.
The dietary supplements of the present invention helps to protect skin by eliminating skin free radical, so as to limit the group of correlation Knit damage.In addition, the dietary supplements of the present invention also enhances the generation of glutathione, this also contributes to protect the skin from certainly By the injury of base.
The dietary supplements of the present invention has direct inhibitory action to cancer cell, so as to contribute to the skin of protection exposure From the cutaneum carcinoma of UV inductions, such as the invasion and attack of melanoma.
10. arthritis.Arthritis be it is a kind of influence body joints inflammatory disease, including backbone, buttocks, knee, shoulder, Ancon, ankle, wrist, pin and pin.Soft tissue inflammation and cartilage inflammation of the dietary supplements of the present invention to arthritis and correlation With direct effect.
11. body weight.According to the research of inventor, dietary supplements of the invention can lose weight increase, so as to Prevention or treatment are fat.
12. skin-whitening.The dietary supplements of the present invention can reduce the activity of melanocyte and increase human body paddy Guang The generation of sweet peptide, so as to bright color.
13. mental health.The dietary supplements of the present invention is by promoting elimination intracerebral amyloid-beta to provide god Through protection, so as to the prevention and treatment for nerve degenerative diseases such as alzheimer disease (AD) etc..Present invention may also apply to Improve mental alertness and strengthen cognitive activities.
14. infection.Experiment in vitro proves that dietary supplements of the invention has the property of antibacterial, therefore the meals of the present invention Eating replenishers can be as the antiseptic applied to prevention enteron aisle and respiratory tract infection.
15. gene clock (Gene Clock).Bioactive substance can in some levels (transcription, RNA it is ripe and steady Determine, translate into protein and posttranslational modification) influence gene expression.Transcript profile occurs on individual cell level and and organism Distinctive genome is completely different.According to the research of inventor, have been reported that now clock Physiological effect gene (CLOCK, Circadian Locomotor Output Cycles Kaput) ratios that methylate of CLOCK CpGs 1 and 8 show, are single Unrighted acid (MUFA) (Milagro, F.I.et relevant with the intake of aldehydes matter with polyunsaturated fatty acid (PUFA) Al.Clock, PER2and BMAL1 DNA methylation：Association with obesity and metabolic Syndrome characteristics and monounsaturated fat intake.Chronobiol.Int.2012, 29,1180-1194.).The hydroxytyrosol component of the present invention has positive role for up-regulation Clock and BMAL1 gene activities. CLOCK genes are substantially responsible for biological clock rhythm and pace of moving things system, and the system instructs 24- circadian rhythm 24s on gene level.Should Gene is related to fat and metabolic syndrome.Researcher has been found that patient with visceratonia abdominal fat obesity and suffered from The patient of metabolic syndrome shows the symptom (timer interrupt) of circadian rhythm disorders, and the illness can produce larger body weight and increase It is subject to and then fat tendency, further development can causes diabetes and atherosclerosis (G ó mez-Santos, C.et Al.Circadian rhythm of clock genes in human adipose explants.Obesity 2009,17, 1481-1485).The dietary supplements of the present invention can regulate and control CLOCK gene activation approach, so as to help to prevent and reduction is made Factor in terms of fat and metabolic syndrome gene.
Causality (Yoshino J, Klein S.A is also likely to be present between the suppression of CLOCK genes and diabetes B Novel Link Between Circadian Clocks and Adipose Tissue Energy Metabolism.Diabetes Jul 2009.62(7)：2175-7).Therefore, the regulation and control to CLOCK genes contribute to prevention or Reduce the seriousness of diabetes B.
The research of inventor displays that the epigenetic variation of CLOCK genes is relevant with breast cancer incidence raising, and this is Because the reduction that methylates of the CLOCK promoter regions of the women to suffer from breast cancer.The dietary supplements of the present invention being capable of actively impact These are mutated and reduce their influences to CLOCK genes, so as to reduce the risk to suffer from breast cancer.
The research of inventor displays that, causes have height between the somatic mutation of colorectal cancer and CLOCK mutation Correlation.The dietary supplements of the present invention helps to stablize CLOCK genes to prevent CLOCK mutation, pre- so as to contribute to CLOCK is mutated in the anti-microsatellite instability target gene for causing colorectal cancer.
Effect of the dietary supplements of the present invention on CLOCK gene expressions can also influence circadian rhythm period, thus regulate and control Sleep pattern.CLOCK and CYCLE albumen (circulating protein) (dBMAL genes) can form dimer, the compound finally with Period (gene of per, Per 1) and timeless (tim genes) promoter combine so as to raise (stimulations) per genes with Tim genes.Therefore, dietary supplements of the invention is the adjusting control agent of CLOCK genes.The regulation activity, which can treat and prevent, sleeps Dormancy rhythm disorder, and can treat and prevent in the associated disorders caused by the travelling of multiple time zones, the i.e. time difference.
16. take exercise and move.Free radical (FR), also referred to as active oxygen and nitrogen material (RONS), one as eubolism Part constantly produces (Bentley, et al.Acute and Chronic Effects of Antioxidant in human body Supplementation on Exercise Performance.Lamprecht M, editor.Antioxidants in Sport Nutrition.Boca Raton(FL)：CRC Press/Taylor&Francis；2015)；(Finaud J, Lac G, Filaire E.Oxidative stress.Sports Medicine.2006；36：327-358).
The FR/RONS of high concentration is harmful to human body, can be caused to the cyto-architectural tight of lipid, protein and DNA Re-oxidation destroys (Halliwell B, Gutteridge J MC.Free Radicals in Biology and Medicine.Oxford：Oxford University Press；1999).
Admittedly, daily exercise and high intensity physical training, including high intensity interval tranining (HIIT), can cause it is a large amount of from By the generation of base.Balance between obtainable antioxidant and oxidant, which has confirmed, can influence contraction of muscle, muscle strength Produce and energy exports (Clarkson P.M, Thompson H.S.Antioxidants：What role do they play in physical activity and healthThe American Journal of Clinical Nutrition.2000；72：637s-646s.).
To the clinical research confirmation that the sportsman for carrying out high intensity interval tranining (HIIt) exercise is carried out when sportsman takes Ability during oral antioxidant produces and exercise behaves oneself best (Lafay S, Jan C, Nardon K.et al.Grape extract improves antioxidant status and physical performance in elite male athletes.Journal of Sports Science and Medicine.2009；8：468-480.)；(Shafat A, Butler P, Jensen R.L, Donnelly A.E.Effects of dietary supplementation with vitamins C and E on muscle function during and after eccentric contractions in humans.European Journal of Applied Physiology.2004；93：196-202.).
HIIT has shown that the ability for the skeletal muscle mitochondrial for suppressing to be referred to as human body " energy plants " to remove and to take exercise Accumulation caused by relevant free radical.
The dietary supplements of the present invention makes muscle performance and strength and energy export and maximize, by the oxygen of musculature The property changed damage is reduced and minimized it, also, maximizes energy production during intake before exercise.By avoiding mitochondria Damage and FR/RONS outputs reduce, and dietary supplements of the invention can also strengthen muscle recovery after exercise.
Embodiment
Following examples are only used for describing embodiment of the present invention, and the scope of the present invention should not be restricted by embodiment Limitation.
Embodiment 1
The composition of the dietary supplements of embodiment 1 is the liquor-saturated solanone extracts of 47mg, 23mg withaferin As extract, 15mg hydroxyls Base tyrosol and 15mg piperings.
Preparation method：
The liquor-saturated solanone extracts of 47mg, 23mg withaferin As extract, 15mg hydroxytyrosols and 15mg piperings are mixed equal It is even, produce the dietary supplements of embodiment 1.
Embodiment 2
The composition of the dietary supplements of embodiment 2 is the liquor-saturated solanone extracts of 40mg, 20mg withaferin As extract, 20mg hydroxyls Base tyrosol and 20mg piperings.
Preparation method：
The liquor-saturated solanone extracts of 40mg, 20mg withaferin As extract, 20mg hydroxytyrosols and 20mg piperings are mixed equal It is even, produce the dietary supplements of embodiment 2.
Embodiment 3
The composition of the dietary supplements of embodiment 3 is the liquor-saturated solanone extracts of 53mg, 27mg withaferin As extract, 10mg hydroxyls Base tyrosol and 10mg piperings.
Preparation method：
The liquor-saturated solanone extracts of 53mg, 27mg withaferin As extract, 10mg hydroxytyrosols and 10mg piperings are mixed equal It is even, produce the dietary supplements of embodiment 3.
Embodiment 4
The composition of the dietary supplements of embodiment 4 is the liquor-saturated solanone extracts of 43mg, 22mg withaferin As extract, 18mg hydroxyls Base tyrosol and 17mg piperings.
Preparation method：
The liquor-saturated solanone extracts of 43mg, 22mg withaferin As extract, 18mg hydroxytyrosols and 17mg piperings are mixed equal It is even, produce the dietary supplements of embodiment 4.
Embodiment 5
The composition of the dietary supplements of embodiment 5 is the liquor-saturated solanone extracts of 50mg, 25mg withaferin As extract, 12mg hydroxyls Base tyrosol and 13mg piperings.
Preparation method：
The liquor-saturated solanone extracts of 50mg, 25mg withaferin As extract, 12mg hydroxytyrosols and 13mg piperings are mixed equal It is even, produce the dietary supplements of embodiment 5.
Embodiment 6
The composition of the dietary supplements of embodiment 6 is the liquor-saturated solanone extracts of 50mg, 25mg withaferin As extract, 17mg hydroxyls Base tyrosol and 18mg piperings.
Preparation method：
The liquor-saturated solanone extracts of 50mg, 25mg withaferin As extract, 17mg hydroxytyrosols and 18mg piperings are mixed equal It is even, produce the dietary supplements of embodiment 6.
Embodiment 7：Oral liquid
The dietary supplement product of embodiment 7 is oral liquid, and the capacity of every oral liquid is 15ml, including：The liquor-saturated eggplants of 47mg Ketone extract, 23mg withaferin As extract, 15mg hydroxytyrosols, 15mg piperings, sucrose 20mg and water 15ml.
Preparation method：By the liquor-saturated solanone extracts of 47mg, 23mg withaferin As extract, 15mg hydroxytyrosols, 15mg peppers Alkali, sucrose 20mg are dissolved in 15ml water, well mixed to obtain mixture；Then the mixture is carried out at homogeneous in homogenizer Reason；Then carry out filling, produce the oral liquid of embodiment 7.
Embodiment 8：Oral liquid
The dietary supplement product of embodiment 8 is oral liquid, and the capacity of every oral liquid is 10ml, including：The liquor-saturated eggplants of 47mg Ketone extract, 23mg withaferin As extract, 20mg hydroxytyrosols, 10mg piperings, sucrose 20mg and water 10ml.
Preparation method：By the liquor-saturated solanone extracts of 47mg, 23mg withaferin As extract, 20mg hydroxytyrosols, 10mg peppers Alkali, sucrose 20mg are dissolved in 10ml water, well mixed to obtain mixture；Then the mixture is carried out at homogeneous in homogenizer Reason；Then carry out filling, produce the oral liquid of embodiment 8.
Embodiment 9：Oral liquid
The dietary supplement product of embodiment 9 is oral liquid, and the capacity of every oral liquid is 5ml, including：The liquor-saturated solanones of 50mg Extract, 25mg withaferin As extract, 15mg hydroxytyrosols, 10mg piperings, sucrose 20mg and water 3ml.
Preparation method：By the liquor-saturated solanone extracts of 50mg, 25mg withaferin As extract, 15mg hydroxytyrosols, 10mg peppers Alkali, sucrose 20mg are dissolved in 3ml water, well mixed to obtain mixture；Then the mixture is carried out at homogeneous in homogenizer Reason；Then carry out filling, produce the oral liquid of embodiment 9.
This document describes the preferred embodiments of the invention, including the optimal side known for inventor for being used to implement the present invention Formula.After description above is read, the change of these preferred embodiments will become aobvious and easy for those skilled in the art See.It is expected that this change, and the side that the present invention can be to specifically describe herein can be suitably used in those skilled in the art Formula is implemented.Therefore, many embodiments of the invention include the institute of theme described in the appended claims that applicable law allows There are modification and equivalent.In addition, unless otherwise indicated herein or more hereafter clear and definite contradiction, otherwise present invention encompasses it is all can Any combinations for the above-mentioned key element that can change.

Claims (15)

1. a kind of dietary supplements, it is characterised in that count in parts by weight, the dietary supplements includes：The liquor-saturated solanone of 40-53 parts Extract, 20-27 part withaferin As extract, 10-20 parts hydroxytyrosol and 10-20 part piperings.
2. dietary supplements according to claim 1, it is characterised in that count in parts by weight, the dietary supplements bag Include：The liquor-saturated solanone extract of 43-51 parts, 21-25 part withaferin As extract, 12-18 parts hydroxytyrosol and 12-18 part piperings.
3. dietary supplements according to claim 1 or 2, it is characterised in that count in parts by weight, the dietary supplements Including 47 parts of liquor-saturated solanone extracts, 23 parts of withaferin A extracts, 15 parts of hydroxytyrosols and 15 parts of piperings.
4. according to the dietary supplements described in claim any one of 1-3, it is characterised in that the liquor-saturated solanone extract and liquor-saturated eggplant Plain A extracts extract from withania somnifera (Withania somnifera) root, and/or withania somnifera (Withania Somnifera) leaf.
5. according to the dietary supplements described in claim any one of 1-4, it is characterised in that the hydroxytyrosol extracts from olive Fruit, olive oil and/or water extract.
6. according to the dietary supplements described in claim any one of 1-5, it is characterised in that the pipering extracts from black Hu Green pepper.
7. according to the dietary supplements described in claim any one of 1-6, it is characterised in that the dietary supplements further wraps Include that dietetic product is upper and/or pharmaceutically acceptable carrier and/or auxiliary material.
8. according to the dietary supplements described in claim any one of 1-7, it is characterised in that the dietary supplements is made into mouth Oral dosage form；Preferably, the peroral dosage form is powder, tablet, capsule, granule, oral liquid or pill.
9. the preparation method of the dietary supplements described in claim any one of 1-8, it is characterised in that the preparation method bag Include：Prepare liquor-saturated solanone extract and withaferin A extract, hydroxytyrosol and pipering according to predetermined ratio；And by liquor-saturated solanone Extract and withaferin A extract, hydroxytyrosol and pipering uniformly mix.
10. preparation method according to claim 9, it is characterised in that the liquor-saturated solanone extract and withaferin A extract Using supercritical CO2Abstraction technique and/or moisture film extraction filtering technique, and/or be impregnated and saturated with being prepared.
11. the preparation method according to claim 9 or 10, it is characterised in that the hydroxytyrosol was extracted using moisture film Filter technology is prepared.
12. according to the preparation method described in claim any one of 9-11, it is characterised in that the pipering uses supercritical CO2 Abstraction technique is prepared.
13. the dietary supplements described in claim any one of 1-8 is in health products or medicine with antioxidation are prepared Application.
14. the dietary supplements described in claim any one of 1-8 is in health products or medicine with antiinflammatory action are prepared Using.
15. dietary supplements described in claim any one of 1-8 prepare prevention and/or treat it is following any one or more Application in the health products or medicine of disease or symptom：Oxidative stress, chronic inflammation, angiocardiopathy (CVD), artery sclerosis, Cancer, Arterial Hypertention, diabetes, Health cost, pollution are drawn caused by hypercholesterolemia and similar relevant disease, cigarette It is the oxidative stress risen and tissue damage, age-related body aging, overweight and fat, bacterium infection, related to sleep Obstacle, to anxiety and stress be related symptom and situation, the damage that triggers of nerve degenerative diseases, skin symptom and ultraviolet Lose.
CN201710588224.2A 2017-07-18 2017-07-18 A kind of dietary supplements and its preparation method and application Pending CN107468696A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103690537A (en) * 2013-12-17 2014-04-02 中国人民解放军军事医学科学院放射与辐射医学研究所 Novel medicinal application of piperine

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103690537A (en) * 2013-12-17 2014-04-02 中国人民解放军军事医学科学院放射与辐射医学研究所 Novel medicinal application of piperine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIRTI VAISHNAVI等: "Differential Activities of the Two Closely Related Withanolides, Withaferin A and Withanone: Bioinformatics and Experimental Evidences", 《PLOS ONE》 *
张书新等: "羟基酪醇的研究进展", 《中国卫生产业》 *

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