CN103597995B - The cultivation method of edible melon plantlet in vitro - Google Patents
The cultivation method of edible melon plantlet in vitro Download PDFInfo
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- CN103597995B CN103597995B CN201310582710.5A CN201310582710A CN103597995B CN 103597995 B CN103597995 B CN 103597995B CN 201310582710 A CN201310582710 A CN 201310582710A CN 103597995 B CN103597995 B CN 103597995B
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Abstract
The invention provides a kind of cultivation method of edible melon plantlet in vitro, belong to agricultural plantation technology field.It solve existing for prior art hang the plantlet in vitro survival rate existed when melon plantlet in vitro is transplanted to field cultivation low, hold susceptible viral and the technical problem such as to infect again.It comprises plantlet in vitro definite value, water normal root water, water Water soluble fertilizer, individual plant draws climing and topdress, step of earthing up.The present invention completes and hangs melon plantlet in vitro by culturing pot to natural transition, and improves and hang the survival rate of melon plantlet in vitro at occurring in nature, reduces the virus infection rate again hanging the melon plantlet in vitro later stage, thus significantly improves the output of hanging melon.
Description
Technical field
The invention belongs to agricultural plantation technology field, relate to a kind of cultivation method of edible melon plantlet in vitro.
Background technology
Hang the perennial herb Climbing Plant that melon (Fructus Trichosanthis Kirilowii) is Angiospermae (Angiospermae), dicotyledonous guiding principle (Dicotyledones), Curcurbitaceae (Cucurbitaceae), Snakegourd Fruit belong to, east China and the ground such as the Changjiang river and the Huanghe valley all can be planted.Hanging melon and fruit real is white and light green or dark green alternate decorative pattern, in salmon pink after ripe.Edible melon dioecism, the breeding method of current main flow has seed to broadcast seedling breeding method and raw block root cuts off breeding method two kinds, seedling breeding is broadcast with seed, the low offspring of reproduction rate is separated seriously, uniformity is poor, fruitless staminiferous plant accounts for more than 70% of total seedling, and seedling cost is high, and virus disease incidence probability high (Seed-borne Virus); Cut off breeding with block root, owing to being subject to the quantitative limitation of block radical, reproductive efficiency is low, and time-consuming, directly affects the expansion of sowing area and the popularization of improved seeds.
People, after years of researches, develop the vegetative propagation technique hanging melon seedling, hang melon plantlet in vitro seedling-cultivating method [application number: 200610161470.1] as Chinese invention patent discloses one.People by experiment room medium cultivate the edible melon plantlet in vitro of detoxification, again plantlet in vitro is transplanted to field, this plantlet in vitro uniformity is good, and the growth of individual plant plantlet in vitro is vigorous, output is high, accept by vast plantation family, be the unique channel of hanging melon industry development at present.But hang melon plantlet in vitro after being transplanted to field, because plantlet in vitro is weak for seedling stage, and plantation family get used to the low level management that block root is transplanted, and therefore survival rate is lower, and the later stage is easily subject to virus and infects, thus the output that impact is overall.
Summary of the invention
The object of the invention is for the problems referred to above, a kind of cultivation method of edible melon plantlet in vitro is provided; Solve existing for prior art hang the plantlet in vitro survival rate existed when melon plantlet in vitro is transplanted to field cultivation low, hold susceptible viral and the technical problem such as to infect again.
For achieving the above object, present invention employs following technical proposal: a kind of cultivation method of edible melon plantlet in vitro, comprises the following steps:
Step 1, plantlet in vitro field planting: the melon plantlet in vitro that hangs in nutritive cube is transplanted in the planting pit in field, with fine earth heap to the above position of plantlet in vitro foundation stipes, described planting pit surrounding is provided with dressing furrow or fertilising cave;
Step 2, waters normal root water: be that 500-800 carbendazim doubly or Tobe body fluid water normal root water, until water is played in the face, cave of planting pit in planting pit by concentration in 2 days after step 1 completes;
Step 3, waters Water soluble fertilizer: in dressing furrow or fertilising cave, water first time Water soluble fertilizer after 3-5 days that complete in step 2, after this watered every 5-7 days and execute once, connect and water more than three times, and described Water soluble fertilizer is concentration is 800-1000 high-nitrogen compound fertilizer doubly;
Step 4, individual plant draws climing: after step 3 completes, and every strain plantlet in vitro only stays a healthy and strong side shoot as major branch, and all the other side shoots and indefinite bud are all dredged and gone, and climbs along frame with manually being drawn by tendril by plantlet in vitro, generates main climing;
Step 5, topdresses, earths up: plantlet in vitro height is after more than 60cm, and in dressing furrow or fertilising Xue Zhong buriing composite fertilizer, every strain plantlet in vitro fertilising 0.25-0.5kg, waters permeable in planting pit after fertilising, and at plantlet in vitro foundation place training fine earth 15-25cm, cultivation completes;
In step 1, described planting pit cave is 15-25cm deeply, and cave spacing is 1.4-1.7m or every mu of cave number is 75-85, and the fine earth that described planting pit inwall has at least 2cm thick, the distance in described planting pit and dressing furrow or cave of applying fertilizer is at more than 15cm.
In the cultivation method of above-mentioned edible melon plantlet in vitro, in step 2, watered toward center from plantlet in vitro periphery when watering normal root water, and after having watered normal root water, earthed up in the face, planting pit cave of playing water.
In the cultivation method of above-mentioned edible melon plantlet in vitro, in step 1, when plantlet in vitro is transplanted to after in planting pit, the plantlet in vitro thing that shelters from heat or light is sheltered from heat or light, shelter from heat or light more than 2 days time.
In the cultivation method of above-mentioned edible melon plantlet in vitro, in step 3, the concentration of at every turn watering Water soluble fertilizer increases progressively successively.
In the cultivation method of above-mentioned edible melon plantlet in vitro, in step 4, described in climb along frame be one or more in iron wire frame, knitting wool frame or thin bamboo strips used for weaving frame, and generate main climing after go out main climing on the indefinite bud sprouted.
Compared with prior art, the invention has the advantages that: complete and hang melon plantlet in vitro by culturing pot to natural transition, and improve and hang the survival rate of melon plantlet in vitro at occurring in nature, reduce the virus infection rate again hanging the melon plantlet in vitro later stage, thus significantly improve the output of hanging melon.
Embodiment
A cultivation method for edible melon plantlet in vitro, comprises the following steps:
Step 1, plantlet in vitro field planting: the melon plantlet in vitro that hangs in nutritive cube is transplanted in the planting pit in field, with fine earth heap to the above position of plantlet in vitro foundation stipes, described planting pit surrounding is provided with dressing furrow or fertilising cave; The distance in described planting pit and dressing furrow or cave of applying fertilizer is at more than 15cm; Described planting pit cave is 15-25cm deeply, and cave spacing is 1.4-1.7m or every mu of cave number is 75-85, and the fine earth that described planting pit inwall has at least 2cm thick, when plantlet in vitro is transplanted to after in planting pit, shelters from heat or light to the plantlet in vitro thing that shelters from heat or light, shelter from heat or light more than 2 days time.
Step 2, waters normal root water: be that 500-800 carbendazim doubly or Tobe body fluid water normal root water, until water is played in the face, cave of planting pit in planting pit by concentration in 2 days after step 1 completes; Watered toward center from plantlet in vitro periphery when watering normal root water, and after having watered normal root water, earthed up in the face, planting pit cave of playing water.
Step 3, waters Water soluble fertilizer: in dressing furrow or fertilising cave, water first time Water soluble fertilizer after 3-5 days that complete in step 2, after this watered every 5-7 days and execute once, connect and water more than three times, and described Water soluble fertilizer is concentration is 800-1000 high-nitrogen compound fertilizer doubly; The concentration of at every turn watering Water soluble fertilizer increases progressively successively.
Step 4, individual plant draws climing: after step 3 completes, and every strain plantlet in vitro only stays a healthy and strong side shoot as major branch, and all the other side shoots and indefinite bud are all dredged and gone, and climbs along frame with manually being drawn by tendril by plantlet in vitro, generates main climing; It is described that to climb along frame be one or more in iron wire frame, knitting wool frame or thin bamboo strips used for weaving frame, and generate main climing after go out main climing on the indefinite bud sprouted.
Step 5, topdresses, earths up; Plantlet in vitro height is after more than 60cm, and in dressing furrow or fertilising Xue Zhong buriing composite fertilizer, every strain plantlet in vitro fertilising 0.25-0.5kg, waters permeable in planting pit after fertilising, and at plantlet in vitro foundation place training fine earth 15-25cm, cultivation completes.
As the preferred scheme of one, in step 1, planting pit cave is 20cm deeply, and cave spacing is 1.6m or every mu of cave number is 80; In step 2, root water was irrigated on the same day; In step 4, whether every day is detected main climing rattan head at dusk and falls down, and should draw main climing rattan head in time to climbing along on frame as fallen down; In steps of 5, plantlet in vitro foundation place training fine earth 20cm.
The present invention completes and hangs melon plantlet in vitro by culturing pot to natural transition, and improve and hang the survival rate of melon plantlet in vitro at occurring in nature, survival rate can reach more than 90%, reduce the virus infection rate again hanging the melon plantlet in vitro later stage, virus again infection rate is reduced to less than 5%, thus significantly improves the output of hanging melon.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.
Claims (5)
1. a cultivation method for edible melon plantlet in vitro, is characterized in that, comprises the following steps:
Step 1, plantlet in vitro field planting: the melon plantlet in vitro that hangs in nutritive cube is transplanted in the planting pit in field, with fine earth heap to the above position of plantlet in vitro foundation stipes, described planting pit surrounding is provided with dressing furrow or fertilising cave;
Step 2, waters normal root water: be that 500-800 carbendazim doubly or Tobe body fluid water normal root water, until water is played in the face, cave of planting pit in planting pit by concentration in 2 days after step 1 completes;
Step 3, waters Water soluble fertilizer: in dressing furrow or fertilising cave, water first time Water soluble fertilizer after 3-5 days that complete in step 2, after this watered every 5-7 days and execute once, connect and water more than three times, and described Water soluble fertilizer is concentration is 800-1000 high-nitrogen compound fertilizer doubly;
Step 4, individual plant draws climing: after step 3 completes, and every strain plantlet in vitro only stays a healthy and strong side shoot as major branch, and all the other side shoots and indefinite bud are all dredged and gone, and climbs along frame with manually being drawn by tendril by plantlet in vitro, generates main climing;
Step 5, topdresses, earths up: plantlet in vitro height is after more than 60cm, and in dressing furrow or fertilising Xue Zhong buriing composite fertilizer, every strain plantlet in vitro fertilising 0.25-0.5kg, waters permeable in planting pit after fertilising, and at plantlet in vitro foundation place training fine earth 15-25cm, cultivation completes;
In step 1, described planting pit cave is 15-25cm deeply, and cave spacing is 1.4-1.7m or every mu of cave number is 75-85, and the fine earth that described planting pit inwall has at least 2cm thick, the distance in described planting pit and dressing furrow or cave of applying fertilizer is at more than 15cm.
2. the cultivation method of edible melon plantlet in vitro according to claim 1, is characterized in that, in step 2, is watered, and earth up to the face, planting pit cave of playing water after having watered normal root water when watering normal root water from plantlet in vitro periphery toward center.
3. the cultivation method of edible melon plantlet in vitro according to claim 1, is characterized in that, in step 1, when plantlet in vitro is transplanted to after in planting pit, shelters from heat or light to the plantlet in vitro thing that shelters from heat or light, shelters from heat or light more than 2 days time.
4. the cultivation method of edible melon plantlet in vitro according to claim 1, is characterized in that, in step 3, the concentration of at every turn watering Water soluble fertilizer increases progressively successively.
5. the cultivation method of edible melon plantlet in vitro according to claim 1, is characterized in that, in step 4, described in climb along frame be one or more in iron wire frame, knitting wool frame or thin bamboo strips used for weaving frame, and generate main climing after go out main climing on the indefinite bud sprouted.
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| CN106134696A (en) * | 2016-06-21 | 2016-11-23 | 益阳市大通湖曾大姐绿色农产品有限公司 | A kind of implantation methods hanging melon |
| CN106613189A (en) * | 2016-12-02 | 2017-05-10 | 罗育进 | Mixing planting method for snake gourd and fritillariacirrhosa |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
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| JP5157286B2 (en) * | 2007-06-29 | 2013-03-06 | 東京電力株式会社 | A method for breeding pot seedlings and planting green grasses |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
Non-Patent Citations (4)
| Title |
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| "吊瓜栽培技术";李宏;《现代农业科技》;20111231(第12期);第144、149页 * |
| "栝楼的栽培技术";康二勇;《畜牧与饲料科学》;20100131;第31卷(第1期);第80-82页 * |
| "长兴吊瓜及其栽培和产业发展";赵伯涛等;《中国野生植物资源》;20041231;第23卷(第6期);第2-3页 * |
| "食用栝楼的生育特性及栽培技术";彭桂福等;《中国西瓜甜瓜》;20030831(第4期);第44-46页 * |
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