CN103585074B - Rabdosia rubescens water extract and application, Rabdosia rubescens extract, skin care item and preparation method thereof - Google Patents
Rabdosia rubescens water extract and application, Rabdosia rubescens extract, skin care item and preparation method thereof Download PDFInfo
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- CN103585074B CN103585074B CN201310634432.3A CN201310634432A CN103585074B CN 103585074 B CN103585074 B CN 103585074B CN 201310634432 A CN201310634432 A CN 201310634432A CN 103585074 B CN103585074 B CN 103585074B
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Abstract
The present invention relates to skin care field, particularly Rabdosia rubescens extract and application, skin care item and preparation method thereof.The preparation method of this Rabdosia rubescens extract is: get Rabdosia rubescens, through alcohol extraction, filtration, distillation, obtains Rabdosia rubescens ethanol extract; Get the filtering residue after filtration, through water extraction, filtration, distillation, obtain Rabdosia rubescens water extract; Get Rabdosia rubescens ethanol extract and the mixing of Rabdosia rubescens water extract, to obtain final product.Skin care item provided by the invention do not add any chemical addition agent, and safety is edible, whitening pro-skin, skin smoothing, have the functions such as anti-miliaria, antipruritic, rash of dispelling, antibacterial, anophelifuge concurrently.
Description
Technical field
The present invention relates to skin care field, particularly Rabdosia rubescens water extract and application, Rabdosia rubescens extract, skin care item and preparation method thereof.
Background technology
The skin of infant is the same with other histoorgan, and structure is not yet grown completely, only has 1/10th of adult skin thick, epidermis is cell monolayer, and in corium, collagen fiber are few, thus lacks flexibility, be easy to permeated by foreign object and rub impaired, and the skin of infant controls soda acid ability.Therefore, the skin of infant is very tender, weak, vulnerable, more easily occurs skin allergy, as dermatosis such as erythema, pimple, vesicle, miliarias.In order to the skin protecting infant tender, in case skin allergy, the head of a family needs the skin-protection product using infant special to baby.
The raw material that the infant cosmetic water of major part sale in the market, skin smoothing frost, floral water adopt is surfactant, pigment, the essence of synthetic, the even also chemical solvent such as ethanol excitatory, butyl ether in formula, having very large harm to infant, is very unsafe for infant skin care.Such as: life-time service surfactant can produce stimulation to skin, make cutaneous ageing, produce damage to a certain degree, severe one can cause Epidermal necrosis, slough to be formed and fracture, surfactant affects the function of human enzymes after entering human body, upset the physiological function of human normal.For the infant that skin is tender, the surfactant in skin care item is more easily percutaneous to be penetrated in body, affects the normal growth promoter of infant.It is too drastic that synthetic food color can cause children's behavior, affects children ' s intelligence development, homergy etc. in interfering bodies.Vanillin is a kind of essence of synthetic, and its interpolation is the demand liking fragrance in order to cater to infant, but research shows, Excess free enthalpy vanillin can add the burden of double kidney, damages the growth of internal organs.Therefore, the skin care item that infant uses should not add the composition such as surfactant, pigment, essence, ethanol, butyl ether of synthetic.
At present, the skin care item listing that existing infant is special.Child's cosmetic water that LiFeiKe Pharmacy Co., Ltd., FuJian Province produces forms through scientific method is refining for primary raw material with Mentholum, thymol, eucalyptus oil, Borneolum Syntheticum, catechu, artificial Calculus Bovis, Fel Serpentis, and this product external is clearly comfortable, safe and non-stimulating.Floral water, emollient cream etc. in The Frog Prince series infant cosmetics, adopt All Pure Nature fruit, plant extract elite and Chinese herbal medicine extract to be that raw material is composite to form, especially Herba portulacae extract is adopted to be main antiinflammatory, irritation, antianaphylaxis components, in Japan to register as medicine.But the kind of the special skin care item of infant is less, therefore, the special skin care item of a kind of novel, safe and effective infant are provided to have important practical significance.
Summary of the invention
In view of this, the invention provides Rabdosia rubescens water extract and application, Rabdosia rubescens extract, skin care item and preparation method thereof.These skin care item do not add any chemical addition agent, and safety is edible, whitening pro-skin, skin smoothing, have the functions such as anti-miliaria, antipruritic, rash of dispelling, antibacterial, anophelifuge concurrently.
In order to realize foregoing invention object, the invention provides following technical scheme:
The invention provides a kind of Rabdosia rubescens water extract, its preparation method is: get Rabdosia rubescens, through alcohol extraction, obtains filtering residue; Get filtering residue, through water extraction, to obtain final product.
Rabdosia rubescens has another name called Rabdosia rubescens, is that Labiatae scented tea belongs to, perennial herb or undershrub, undershrub, is that Labiatae Rabdosia plant is cracked rice fork mutation.Medicinal part is Herb, nature and flavor are bitter, sweet, be slightly cold, there is heat-clearing and toxic substances removing, anti-inflammatory analgetic, stomach invigorating invigorated blood circulation, the effect such as antibacterial, antitumor, cure mainly laryngopharynx swelling and pain, tonsillitis, headache due to common cold, tracheitis, chronic hepatitis, articular rheumatic pain, snake bite and insect sting, have mitigation to the esophageal carcinoma, breast carcinoma, rectal cancer etc.Research shows, containing compound effective ingredient such as rubescensine A, B prime, ursolic acid, oleanolic acid, chlorophylls in Rabdosia rubescens extract.These compound effective ingredient show stronger antibacterial action to Staphylococcus albus, group B streptococcus, bacterium flexneri, and the minimal inhibitory concentration (MIC) of rubescens total diterpenoids to staphylococcus aureus, Staphylococcus albus is 1:12800; The MIC of rubescensine A to B-mode, α-hemolytic streptococcus and streptococcus pneumoniae is 1:25600; Rubescensine B is l:51200 to the staphylococcic MIC of white.The research that the effects such as Rabdosia rubescens is antibacterial, antibacterial, anticancer are used for Drug therapy have accumulated a large amount of experiences and achieves much surprising achievement.In the present invention, research finds in Rabdosia rubescens water extract containing a large amount of rosmarinic acid, rosmarinic acid has very strong non-oxidizability, contribute to the cell damage preventing free radical from causing, its anti-oxidant vigor is better than vitamin E, the effective slow down aging of energy, has the functions such as obvious human body cell reparative regeneration, horny layer softening, skin Caring whitening.Rabdosia rubescens water extract is applied in skin care item by the present invention, makes skin care item provided by the invention have the functions such as obvious human body cell reparative regeneration, horny layer softening, skin Caring whitening.
As preferably, Rabdosia rubescens adopts state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens.
As preferably, the mass percentage concentration of the ethanol water that alcohol extraction is used is 60% ~ 95%.
Preferably, the mass percentage concentration of ethanol water is 80%.
As preferably, the temperature of water extraction is at 50 ~ 90 DEG C.
Present invention also offers this Rabdosia rubescens water extract and prepare the application in antioxidant.
Present invention also offers a kind of skin care item, comprise acceptable auxiliary agent on Rabdosia rubescens extract and cosmeceutical; Rabdosia rubescens extract comprises Rabdosia rubescens ethanol extract and Rabdosia rubescens water extract provided by the invention.
Rabdosia rubescens water extract is applied in skin care item by the present invention, these skin care item can be made to have the functions such as obvious human body cell reparative regeneration, horny layer softening, skin Caring whitening, expand the curative effect range of application of product.
Rabdosia rubescens ethanol extract is also applied in skin care item by the present invention, makes these skin care item have effect of anti-inflammation, promoting blood circulation and stopping pain, anti-cancer and cancer-preventing.
The preparation method of this Rabdosia rubescens extract is: get Rabdosia rubescens, through alcohol extraction, obtains Rabdosia rubescens ethanol extract and filtering residue; Get filtering residue, through water extraction, obtain Rabdosia rubescens water extract; Get Rabdosia rubescens ethanol extract and the mixing of Rabdosia rubescens water extract, to obtain final product; As preferably, Rabdosia rubescens adopts state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens; As preferably, the mass percentage concentration of the ethanol water that alcohol extraction is used is 60% ~ 95%; Preferably, the mass percentage concentration of ethanol water is 80%; As preferably, the temperature of water extraction is at 50 ~ 90 DEG C.
In embodiments more provided by the invention, skin care item are cosmetic water.
As preferably, on cosmeceutical, acceptable auxiliary agent is selected from bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier or water.
As preferably, the mass ratio of Rabdosia rubescens extract, bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier and water is (1 ~ 5): (5 ~ 30): (4 ~ 40): (0.5 ~ 2): (0.2 ~ 3): (10 ~ 30): (6 ~ 25): (20 ~ 80).
In embodiments more provided by the invention, the preparation method of bean sprout extract is: get legume seeds, through soaking, germinateing, squeeze the juice, cell wall breaking, filtration, obtain bean sprout ultrafiltration solution and bean sprout filtering residue; Get bean sprout filtering residue, through alcohol extraction, remove ethanol, obtain bean sprout ethanol extract; Get bean sprout ethanol extract and the mixing of bean sprout ultrafiltration solution, to obtain final product.
In the process of germinateing, multiple beneficial can be there is in the change of human body, as partially protein can resolve into the free amino acid be easily absorbed by the body in legume seeds; The mineral such as more phosphorus, zinc can be discharged; The content of vitamin substances also can increase greatly.In the present invention, legume seeds is soaked in water, germinate, squeeze the juice, cell wall breaking is refined, filtering residue adopts the molten active matter of ethanol extraction oil, obtained bean sprout ethanol extract and bean sprout ultrafiltration solution, the two merges, effective active composition in bean sprout is fully extracted, be applied in skin-care products for infant, this skin-care products for infant is made to contain abundant skin care ingredient, such as, cellulose, vitamin and mineral have beauty treatment toxin expelling, disappear fat relieving constipation, antioxidative effect, vitamin C, vitamin E, the nutritions such as chlorophyll have skin care, effect of prophylaxis of tumours, Concha Ostreae alkali and different Concha Ostreae alkali, have remarkable cleaning, moistening effect, removes sebum function remarkable, effectively can remove the unclean thing in skin, more can thoroughly remove deep skin refuse, make skin shine cleaning, transparent brilliance, natural polysaccharides (Polysaccharide) forms moisture film transparent, springy in skin surface, and skin is moistened.
As preferably, legume seeds is Semen phaseoli radiati, Semen sojae atricolor, Semen Glycines or Semen Phaseoli.
Preferably, legume seeds is Semen phaseoli radiati.
Semen phaseoli radiati is the mature seed of leguminous herbaceous plant's Semen phaseoli radiati (PhaseolusradiatusL.), has the cultivation history of more than 2,000 year in China.In the process of germinateing, multiple beneficial can be there is in the change of human body, as partially protein can resolve into the free amino acid be easily absorbed by the body, seven times of the former content of Semen phaseoli radiati can be reached in Semen phaseoli radiati; The mineral such as more phosphorus, zinc can be discharged; The content of vitamin substances also can increase greatly, wherein vitamin B
2add 2-4 doubly, carotene adds 2-3 doubly, and vitamin C adds 6%, folic acid and vitamin B
6also increase to some extent.The cellulose wherein contained, vitamin and mineral have beauty treatment toxin expelling, the fat relieving constipation that disappears, antioxidative effect, and the nutritions such as the vitamin C contained, vitamin E, chlorophyll have effect of skin care, prophylaxis of tumours; The Concha Ostreae alkali contained and different Concha Ostreae alkali, have remarkable cleaning, moistening effect, removes sebum function remarkable, effectively can remove the unclean thing in skin, more can thoroughly remove deep skin refuse, make skin shine cleaning, transparent brilliance; Natural polysaccharides (Polysaccharide) wherein forms moisture film transparent, springy in skin surface, and skin is moistened.In the present invention, soaked by Semen phaseoli radiati, germinate, squeeze the juice, cell wall breaking refines in water, filtering residue adopts the molten active matter of ethanol extraction oil, obtained Semen Phaseoli radiati Germinatus ethanol extract and Semen Phaseoli radiati Germinatus ultrafiltration solution, the two merges, and is fully extracted by the effective ingredient in Semen Phaseoli radiati Germinatus, in skin-care products for infant.
As preferably, the time that legume seeds soaks is 0.5 ~ 2h.
Preferably, the time that legume seeds soaks is 1h.
As preferably, the time that legume seeds germinates after soaking is 4 ~ 5 days.
In embodiments more provided by the invention, the preparation method of Fructus Cucumidis sativi extract is: get Fructus Cucumidis sativi and be placed in water and soak, through squeezing the juice, cell wall breaking, filtration, obtain Fructus Cucumidis sativi ultrafiltration solution and Fructus Cucumidis sativi filtering residue; Get Fructus Cucumidis sativi filtering residue, through alcohol extraction, remove ethanol, obtain nonadienol extract; Get nonadienol extract and the mixing of Fructus Cucumidis sativi ultrafiltration solution, to obtain final product.
Fructus Cucumidis sativi rich in vitamin E and cucumber chitinase.Wherein, vitamin E has the effect of free radical resisting oxidation; Cucumber chitinase is a kind of enzyme having very strong biological activity, effectively can promote the metabolism of body, skin expansion blood capillary, blood circulation promoting, strengthens the redox of skin, has skin moistening, unfolds wrinkle, effect of careful pore.In the present invention, Fructus Cucumidis sativi is placed in water soaks, squeezes the juice, cell wall breaking is refined, obtain Fructus Cucumidis sativi ultrafiltration solution, filtering residue adopts the molten active matter of ethanol extraction Fructus Cucumidis sativi oil.This method ensure that the extraction for effective active composition multiple in Fructus Cucumidis sativi, make containing abundant skin care ingredient in skin care item provided by the invention, as vitamin E and cucumber chitinase.
As preferably, the Fructus Cucumidis sativi adopted in the present invention is Gherkin.Cucumber chitinase content more horn of plenty wherein.
In embodiments more provided by the invention, the time that Fructus Cucumidis sativi is soaked is 0.5 ~ 2h.
As preferably, the time that Fructus Cucumidis sativi is soaked is 1h.
Oligochitosan (ChitosanOligosaccharides) refers to that 2-10 glucosamine is with β-1; the oligosaccharide that 4-glycosidic bond is formed by connecting, it is normally obtained through biological enzymolysis by the product chitosan (Chitosan) that chitin (Chitin) is deacetylated.Oligochitosan derives from bio-extract, its special molecular structure determines oligochitosan and has excellent moisturizing moisturization, skin surface antibacterial can be suppressed simultaneously, activate epidermal cell, strengthen skin elasticity, compared with traditional moisturizing humidizer, moistening effect is better, thus is applied to beauty treatment, cosmetic, skin protection, anti-ageing field of fine chemical of waiting for a long time.1881, the most famous pharmacy man of ancient Chinese---" Compendium of Materia Medica " of the holy Li Shizhen (1518-1593 A.D.) of medicine was recorded: break Carapax Eriocheir sinensis into pieces, make dumpling and eat, effective to comedo, swollen thing.The states such as Japan and Korea S. utilize the specific function of oligochitosan to develop multiple daily chemical products, as " Essence ", " Cream " etc.The degree of polymerization that the present invention adopts marine organisms to obtain through enzyme solution lower than 20 oligosaccharide, there is very large biological activity, for its damping moisturizing can be played in the outer skin care item of infants, improve the effect of immunity, and electromagnetic radiation and solar radiation can be prevented.
In embodiments more provided by the invention, oligochitosan avenges Carapax Eriocheir sinensis by deep-sea to obtain through enzymatic degradation.
As preferably, the degree of polymerization of oligochitosan is lower than 20.
Collagen protein (Collagen) is a kind of white, opaque, unbranched celloglobulin, is important functional protein.Collagen protein has good biological characteristics and reduced immunogenicity, and it can be used as the holder of tissue, exercises normal function and have important function to wound reparation to cell, tissue and even organ.In cosmetics industry, collagen and hydrolytic collagen have good moisture retention owing to being rich in the hydrophilic radical such as hydroxyl, amino." micromolecular collagen peptide " that collagen protein resolves into can reach 500 dalton (atomic mass unit), can 100% to be absorbed by the body utilization.Still can keep the moisture of its spontaneous weight 45% when relative humidity 70%, add collagen concentration in cosmetics when reaching 0.01%, the required all moisture of skin can be supplied; Collagen protein, because of good with the affinity of surrounding tissue, has the effect of repair tissue.
In embodiments more provided by the invention, collagen protein is formed through the directed shearing technique of enzyme is refining by the cod skin at deep-sea.
Moisture content in glycerol reliable absorbtion air reaches skin moisturizing, has moisturizing, humectation, also has the special efficacies such as high activity, antioxidation, short alcoholization.Therefore, in embodiments more provided by the invention, moisture-regulating agent is glycerol.
Preferably, glycerol is edible glycerol.
Sorbitol has good moistening, emulsifying, peptizaiton and preferably foam characteristics.In the present invention, sorbitol can play maintenance moisture content, prevent xerodermatic effect, without excitatory, and does not have greasy and adhesion sensation, has also played emulsifying, suspension, stable effect in the present invention.Therefore, in embodiments more provided by the invention, wetting emulsifier is sorbitol.
Preferably, sorbitol is edible sorbitol.
As preferably, in skin care item and in the preparation process of bean sprout extract and Fructus Cucumidis sativi extract, water used is preferably the water containing zinc ion.
Be transported in the tap water of user terminal and generally all contain chlorine residue, chlorine is a kind of strong oxidizer, is very easily oxidized the keratoprotein of skin, once skin is for a long time by oxychloride, easily causes various dermatosis.Meanwhile, the chlorine residue in tap water creates carcinogenic chloroform through high temperature action.When boiling 100 DEG C, toxicity is the highest.Distilled water is very clean, but the beneficial element in water is all gone.Therefore, in embodiments more provided by the invention, the water containing zinc ion is by the rich zinc active-oxygen-water of the machine-processed mineralising obtained of mineralising rich zinc active-oxygen-water water.Mineralising rich zinc active-oxygen-water water machine adopts functionalization natural energy Ceramic Balls technology to obtain, and functionalization natural energy Ceramic Balls technology can also calcium, magnesium ion in activated water.The rich zinc active-oxygen-water of mineralising is through 8 kinds of filter element filtering gained to tap water, the effect of 8 kinds of filter elements is respectively turbidity removal, tender taste, ultrafiltration, activation, weak base, Fu Xin, mineralising, antibacterial, be characterized in that there is subset water-bound, be rich in trace element and be especially rich in zinc ion, not containing heavy metal.Calcium ions and magnesium ions in tap water is further activated and non-scaling through the filtration of several filter element, is a kind of heavy-oxygen-enriched water with energy-activated mineralising.The present invention adopts this water to clean legume seeds and Fructus Cucumidis sativi, on the basis of removing harmful substance, can retain and promote the healthy trace elements with household in water to greatest extent, allowing water become a kind of nutritional solution.
Present invention also offers the preparation method of these skin care item, comprise the steps:
Get Rabdosia rubescens, through alcohol extraction, obtain Rabdosia rubescens ethanol extract and filtering residue; Get filtering residue, through water extraction, obtain Rabdosia rubescens water extract; Get Rabdosia rubescens ethanol extract and the mixing of Rabdosia rubescens water extract, obtain Rabdosia rubescens extract;
Obtain acceptable auxiliary agent on cosmeceutical;
Get acceptable auxiliary agent mixing on Rabdosia rubescens extract and cosmeceutical, to obtain final product.
As preferably, Rabdosia rubescens adopts state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens.
As preferably, the mass percentage concentration of the ethanol water that alcohol extraction is used is 60% ~ 95%.
Preferably, the mass percentage concentration of ethanol water is 80%.
As preferably, the temperature of water extraction is at 50 ~ 90 DEG C.
In skin care item preparation method, these skin care item comprise acceptable auxiliary agent on Rabdosia rubescens extract provided by the invention and cosmeceutical.
In embodiments more provided by the invention, skin care item are cosmetic water.
In skin care item preparation method, as preferably, on cosmeceutical, acceptable auxiliary agent is selected from bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier or water.
Skin care item provided by the invention are cosmetic water, and formula comprises Rabdosia rubescens extract, bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier and water.
As preferably, the mass ratio of Rabdosia rubescens extract, bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier and water is (1 ~ 5): (5 ~ 30): (4 ~ 40): (0.5 ~ 2): (0.2 ~ 3): (10 ~ 30): (6 ~ 25): (20 ~ 80).
In some embodiments that skin care item preparation method provides, the preparation method of bean sprout extract is: get legume seeds, through soaking, germinateing, squeeze the juice, cell wall breaking, filtration, obtain bean sprout ultrafiltration solution and bean sprout filtering residue; Get bean sprout filtering residue, through alcohol extraction, remove ethanol, obtain bean sprout ethanol extract; Get bean sprout ethanol extract and the mixing of bean sprout ultrafiltration solution, to obtain final product.
As preferably, legume seeds is Semen phaseoli radiati, Semen sojae atricolor, Semen Glycines or Semen Phaseoli.
Preferably, legume seeds is Semen phaseoli radiati.
As preferably, the time that legume seeds soaks is 0.5 ~ 2h.
Preferably, the time that legume seeds soaks is 1h.
As preferably, the time that legume seeds germinates after soaking is 4 ~ 5 days.
In some embodiments that skin care item preparation method provides, the preparation method of Fructus Cucumidis sativi extract is: get Fructus Cucumidis sativi and be placed in water and soak, through squeezing the juice, cell wall breaking, filtration, obtain Fructus Cucumidis sativi ultrafiltration solution and Fructus Cucumidis sativi filtering residue; Get Fructus Cucumidis sativi filtering residue, through alcohol extraction, remove ethanol, obtain nonadienol extract; Get nonadienol extract and the mixing of Fructus Cucumidis sativi ultrafiltration solution, to obtain final product.
As preferably, the Fructus Cucumidis sativi adopted in the present invention is Gherkin.
In embodiments more provided by the invention, the time that Fructus Cucumidis sativi is soaked is 0.5 ~ 2h.
As preferably, the time that Fructus Cucumidis sativi is soaked is 1h.
In embodiments more provided by the invention, oligochitosan avenges Carapax Eriocheir sinensis by deep-sea to obtain through enzymatic degradation.
As preferably, the degree of polymerization of oligochitosan is lower than 20.
In embodiments more provided by the invention, moisture-regulating agent is glycerol.
Preferably, glycerol is edible glycerol.
In embodiments more provided by the invention, wetting emulsifier is sorbitol.
Preferably, sorbitol is edible sorbitol.
As preferably, in skin care item and in the preparation process of bean sprout extract and Fructus Cucumidis sativi extract, water used is preferably the water containing zinc ion.
In embodiments more provided by the invention, the water containing zinc ion is by the rich zinc active-oxygen-water of the machine-processed mineralising obtained of mineralising rich zinc active-oxygen-water water.
The invention provides Rabdosia rubescens water extract and application, Rabdosia rubescens extract, skin care item and preparation method thereof.The preparation method of this Rabdosia rubescens water extract is: get Rabdosia rubescens, through alcohol extraction, obtains filtering residue; Get filtering residue, through water extraction, to obtain final product.By the detection experiment of effective ingredient, Rabdosia rubescens water extract provided by the invention contains abundant rosmarinic acid component; By whitening effect test display, cosmetic water provided by the invention has significant whitening effect, its energy check melanin tumor cell growth, and can reduce the melanin content in cell; By fungistatic effect test display, cosmetic water provided by the invention has obvious inhibitory action to escherichia coli and staphylococcus aureus; The chemical composition such as surfactant, pigment, essence, ethanol, butyl ether of skin care item provided by the invention not containing synthetic is a kind of pure natural, green, edible product.As can be seen here, skin care item provided by the invention do not add any chemical addition agent, and safety is edible, have whitening, the function such as antibacterial.
Detailed description of the invention
The invention discloses Rabdosia rubescens water extract and application, Rabdosia rubescens extract, skin care item and preparation method thereof, those skilled in the art can use for reference present disclosure, and suitable improving technique parameter realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope methods and applications as herein described are changed or suitably change with combination, realize and apply the technology of the present invention.
In Rabdosia rubescens water extract provided by the invention and application, Rabdosia rubescens extract, skin care item and preparation method thereof, raw materials used or auxiliary agent all can be buied by market.Wherein, oligochitosan is purchased from Bo Zhihui power bio tech ltd, Qingdao of National Marine Medicine Engineering Technology Research Center, and it adopts deep-sea snow Carapax Eriocheir sinensis to be obtained by enzymatic degradation, and its degree of polymerization is lower than 20.
Below in conjunction with embodiment, set forth the present invention further:
The preparation of embodiment 1 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 60% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 90 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
The preparation of embodiment 2 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 65% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 80 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
The preparation of embodiment 3 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 70% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 70 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
The preparation of embodiment 4 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 75% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 65 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
The preparation of embodiment 5 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 80% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 60 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
The preparation of embodiment 6 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 85% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 55 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
The preparation of embodiment 7 Rabdosia rubescens water extract
The preparation method of Rabdosia rubescens water extract: adopt state guarantee Chinese herbal medicine-Jiyuan Rabdosia rubescens, through 95% ethanol water leaching, then through squeezing, distillation, obtains Rabdosia rubescens ethanol extract.Residue high energy rich zinc mineralized water heating leaching, leaching temperature, at 50 DEG C, squeezes, distills, obtains Rabdosia rubescens water extract.
Embodiment 8 effective substance is tested
According to high performance liquid chromatography (Chinese Pharmacopoeia version in 2010 annex VI D), the rosmarinic acid in the obtained Rabdosia rubescens water extract of embodiment 1 to 7 is carried out to the mensuration of content.
Chromatographic condition and system suitability: take octadecylsilane chemically bonded silica as filler; With acetonitrile-0.2% phosphoric acid solution (22:78) for mobile phase; Determined wavelength is 329nm.Number of theoretical plate calculates should be not less than 4000 by rosmarinic acid peak.
The preparation of reference substance solution: get rosmarinic acid reference substance appropriate, accurately weighed, add 50% methanol and make the solution of every 1mL containing 30 μ g, to obtain final product.
The preparation of need testing solution: precision measures the obtained Rabdosia rubescens water extract 0.5g of embodiment 1 to 7, puts in 50mL volumetric flask, adds 50% methanol dilution to scale, shake up, leave standstill, filter, get subsequent filtrate, to obtain final product.
Assay method: accurate absorption reference substance solution and each 10 μ L of need testing solution respectively, injection liquid chromatography, measures, to obtain final product.The every 1g of water extract contains Rabdosia rubescens with rosmarinic acid (C
18h
16o
8) meter.
After testing, in the Rabdosia rubescens water extract that embodiment 1 to 7 is obtained, the content of rosmarinic acid is as shown in table 1.
The measurement result of table 1 rosmarinic acid contents
Table 1 result shows, in the Rabdosia rubescens water extract that embodiment 1 to 7 is obtained, the content of rosmarinic acid is respectively 2.6mg/g, 2.8mg/g, 2.9mg/g, 3.2mg/g, 3.1mg/g, 2.8mg/g, 2.7mg/g, shows the rich content of rosmarinic acid in the Rabdosia rubescens water extract that embodiment 1 to 7 is obtained.
The preparation of embodiment 9 cosmetic water
The prescription of cosmetic water is as shown in table 2.
The prescription of table 2 cosmetic water
Adopt fresh Semen phaseoli radiati, clean up with water, then soak 0.5 hour.Be put into after draining in large-scale sprouting machine and germinate 5 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 0.5 hour.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 287kg high energy, open and stir, add bean sprout extract 100kg, Fructus Cucumidis sativi extract 200kg, glycerol 200kg, sorbitol 100kg successively, obtain the first mixture; The rich zinc mineralized water of 20kg high energy, 50kg sorbitol, Rabdosia rubescens extract 30kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 8kg and collagen protein 5kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
The preparation of embodiment 10 cosmetic water
The prescription of cosmetic water is as shown in table 3.
The prescription of table 3 cosmetic water
Adopt fresh Semen phaseoli radiati, clean up with water, then soak 1 hour.Be put into after draining in large-scale sprouting machine and germinate 4 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 1 hour.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 287kg high energy, open and stir, add bean sprout extract 100kg, Fructus Cucumidis sativi extract 200kg, glycerol 150kg, sorbitol 150kg successively, obtain the first mixture; The rich zinc mineralized water of 25kg high energy, 50kg sorbitol, Rabdosia rubescens extract 25kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 8kg and collagen protein 5kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
The preparation of embodiment 11 cosmetic water
The prescription of cosmetic water is as shown in table 4.
The prescription of table 4 cosmetic water
Adopt fresh Semen phaseoli radiati, clean up with water, then soak 1.5 hours.Be put into after draining in large-scale sprouting machine and germinate 5 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 1.5 hours.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 287kg high energy, open and stir, add bean sprout extract 150kg, Fructus Cucumidis sativi extract 150kg, glycerol 150kg, sorbitol 150kg successively, obtain the first mixture; The rich zinc mineralized water of 25kg high energy, 50kg sorbitol, Rabdosia rubescens extract 25kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 8kg and collagen protein 5kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
The preparation of embodiment 12 cosmetic water
The prescription of cosmetic water is as shown in table 5.
The prescription of table 5 cosmetic water
Adopt fresh Semen phaseoli radiati, clean up with water, then soak 2 hours.Be put into after draining in large-scale sprouting machine and germinate 4 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 2 hours.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 287kg high energy, open and stir, add bean sprout extract 150kg, Fructus Cucumidis sativi extract 150kg, glycerol 150kg, sorbitol 150kg successively, obtain the first mixture; The rich zinc mineralized water of 27kg high energy, 50kg sorbitol, Rabdosia rubescens extract 20kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 8kg and collagen protein 8kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
The preparation of embodiment 13 cosmetic water
The prescription of cosmetic water is as shown in table 6.
The prescription of table 6 cosmetic water
Adopt fresh Semen Phaseoli, clean up with water, then soak 0.5 hour.Be put into after draining in large-scale sprouting machine and germinate 5 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 0.5 hour.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 540kg high energy, open and stir, add bean sprout extract 100kg, Fructus Cucumidis sativi extract 400kg, glycerol 300kg, sorbitol 30kg successively, obtain the first mixture; The rich zinc mineralized water of 60kg high energy, 30kg sorbitol, Rabdosia rubescens extract 10kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 5kg and collagen protein 5kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
The preparation of embodiment 14 cosmetic water
The prescription of cosmetic water is as shown in table 7.
The prescription of table 7 cosmetic water
Adopt fresh Semen sojae atricolor, clean up with water, then soak 1 hour.Be put into after draining in large-scale sprouting machine and germinate 4 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 1 hour.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 720kg high energy, open and stir, add bean sprout extract 300kg, Fructus Cucumidis sativi extract 40kg, glycerol 100kg, sorbitol 150kg successively, obtain the first mixture; The rich zinc mineralized water of 80kg high energy, 50kg sorbitol, Rabdosia rubescens extract 30kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 8kg and collagen protein 30kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
The preparation of embodiment 15 cosmetic water
The prescription of cosmetic water is as shown in table 8.
The prescription of table 8 cosmetic water
Adopt fresh Semen Glycines, clean up with water, then soak 2 hours.Be put into after draining in large-scale sprouting machine and germinate 5 days, bean sprout is smashed on plant disintegrating machine, then drains on squeezer, dewatering.Squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain bean sprout ultrafiltration solution by mocromembrane; Filtering residue and squeezing bean sprout residue merge, and extract, steam except ethanol, obtain bean sprout ethanol extract by soaked in absolute ethyl alcohol.Get bean sprout ultrafiltration solution and the merging of bean sprout ethanol extract, obtained bean sprout extract, stand-by.
Adopt fresh Gherkin, clean up with water, then soak 2 hours.Be put into after draining on colloid mill and grind, squeezing the juice of obtaining is carried out cell wall breaking and is refined, and is filtered, obtain Fructus Cucumidis sativi ultrafiltration solution by mocromembrane; Get Fructus Cucumidis sativi filtering residue soaked in absolute ethyl alcohol to extract, steam except ethanol, obtain nonadienol extract.Get nonadienol extract and the merging of Fructus Cucumidis sativi ultrafiltration solution, stand-by.
In 1500 liters of stainless steel cauldrons of sealing, add the rich zinc mineralized water of 180kg high energy, open and stir, add bean sprout extract 50kg, Fructus Cucumidis sativi extract 200kg, glycerol 200kg, sorbitol 200kg successively, obtain the first mixture; The rich zinc mineralized water of 20kg high energy, 50kg sorbitol, Rabdosia rubescens extract 50kg is added in the rustless steel emulsify at a high speed cutter of 500 liters, mixing, then add oligochitosan powder 20kg and collagen protein 2kg, emulsifying is even, the dispersed time is 1.5 hours, obtains the second mixture; The second obtained mixture is joined in the stainless steel cauldron that the first mixture is housed, continues to be stirred to evenly, then connect high pressure homogenizer homogenizing, to obtain final product.
Embodiment 16 whitening effect is tested
For evaluating the whitening effect of cosmetic water provided by the invention, test adopts B-16 melanoma cell strain to be subject cell, measures the white-skinned face function of the cosmetic water that the embodiment of the present invention 9 to 15 provides.
B-16 melanoma cell strain grows to subconfluence, goes down to posterity through 0.25% trypsinization, uses I640 culture fluid, is placed in CO
2incubator 37 DEG C, 5%CO
2, saturated humidity environment is cultivated.Same passage cell is taken from experiment, and initial inoculation cell density is 10
5individual/about mL, after cell inoculation 2h, is equally divided into two groups.One group is matched group, does not add any reagent; Another group is test group, adds cosmetic water (tested material) 1mL that the embodiment of the present invention 9 provides, changes culture fluid and tested material, harvesting after continuous culture 5d in 3d.With Trypan Blue counting cells sum, then melanin content mensuration is carried out to cell.Result of the test is as shown in table 9.
Melanoma cell quantity after table 95d
Table 9 result shows, and the cosmetic water that the embodiment of the present invention 9 provides can obviously suppress B-16 melanoma cell growth, and melanin content in melanoma cell is obviously reduced.Result shows that the cosmetic water that the embodiment of the present invention 9 provides has significant whitening effect, its energy check melanin tumor cell growth, and can reduce the melanin content in cell.
The cosmetic water that getting the embodiment of the present invention 10 to 15 provides carries out the mensuration of white-skinned face function, the result of result and embodiment 9 is close, show that the cosmetic water that the embodiment of the present invention 10 to 15 provides has significant whitening effect, its energy check melanin tumor cell growth, and the melanin content in cell can be reduced.
As can be seen here, cosmetic water provided by the invention has significant whitening effect, its energy check melanin tumor cell growth, and can reduce the melanin content in cell.
Embodiment 17 fungistatic effect is tested
Test is divided into matched group and test group, and matched group is left intact, and test group adopts cosmetic water provided by the invention to process, and investigates its fungistatic effect.
Concrete operations are: be inoculated in 5mL fluid medium respectively by escherichia coli and staphylococcus aureus, 37 DEG C, 150r/min cultivates 24h, then transfer 1 time respectively, cultivate 24h, obtain the bacterium liquid of activation; The bacterium liquid of activation is diluted to l0 doubly with aquesterilisa, for subsequent use.The cosmetic water 1mL(100 μ g/mL that Example 9 provides), add in the culture dish of test group, pour solid medium into.Directly solid medium is poured in the culture dish of matched group.After culture medium solidifying, get 100 μ L and be diluted to the bacterium liquid even spread that l0 doubly activates, proceed to 37 DEG C of incubators and cultivate 24h, carry out colony counting.Result is as shown in table 10.
Table 10 fungistatic effect testing result
Table 10 result shows, colony counts on matched group flat board is too much, all cannot count, and escherichia coli and S. aureus colonies number are respectively 109cfu, 12cfu on flat board after the cosmetic water process that the embodiment of the present invention 9 provides, result shows that the cosmetic water that the embodiment of the present invention 9 provides has obvious inhibitory action (P<0.01) to escherichia coli and staphylococcus aureus.
The cosmetic water that getting the embodiment of the present invention 10 to 15 provides carries out fungistatic effect test, and the result of result and embodiment 9 is close, shows that the cosmetic water that the embodiment of the present invention 10 to 15 provides has obvious inhibitory action to escherichia coli and staphylococcus aureus.
As can be seen here, cosmetic water provided by the invention has obvious inhibitory action to escherichia coli and staphylococcus aureus.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. skin care item, is characterized in that, are made up of auxiliary agent acceptable on Rabdosia rubescens extract and cosmeceutical; Described Rabdosia rubescens extract comprises Rabdosia rubescens ethanol extract and Rabdosia rubescens water extract;
Described Rabdosia rubescens water extract is prepared in accordance with the following methods:
Get Rabdosia rubescens, through alcohol extraction, obtain filtering residue; Get described filtering residue, through water extraction, to obtain final product; Described skin care item are cosmetic water; On described cosmeceutical, acceptable auxiliary agent is bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier and water;
The mass ratio of described Rabdosia rubescens extract, described bean sprout extract, described Fructus Cucumidis sativi extract, described oligochitosan, described collagen protein, described moisture-regulating agent, described wetting emulsifier and described water is (1 ~ 5): (5 ~ 30): (4 ~ 40): (0.5 ~ 2): (0.2 ~ 3): (10 ~ 30): (6 ~ 25): (20 ~ 80).
2. skin care item according to claim 1, is characterized in that, the preparation method of described bean sprout extract is: get legume seeds, through soaking, germinateing, squeeze the juice, cell wall breaking, filtration, obtain bean sprout ultrafiltration solution and bean sprout filtering residue; Get described bean sprout filtering residue, through alcohol extraction, remove ethanol, obtain bean sprout ethanol extract; Get described bean sprout ethanol extract and the mixing of described bean sprout ultrafiltration solution, to obtain final product.
3. skin care item according to claim 1, is characterized in that, the preparation method of described Fructus Cucumidis sativi extract is: get Fructus Cucumidis sativi and be placed in water and soak, through squeezing the juice, cell wall breaking, filtration, obtain Fructus Cucumidis sativi ultrafiltration solution and Fructus Cucumidis sativi filtering residue; Get described Fructus Cucumidis sativi filtering residue, through alcohol extraction, remove ethanol, obtain nonadienol extract; Get described nonadienol extract and the mixing of described Fructus Cucumidis sativi ultrafiltration solution, to obtain final product.
4. the preparation method of skin care item according to any one of claims 1 to 3, is characterized in that, comprise the steps:
Get Rabdosia rubescens, through alcohol extraction, obtain Rabdosia rubescens ethanol extract and filtering residue; Get described filtering residue, through water extraction, obtain Rabdosia rubescens water extract; Get described Rabdosia rubescens ethanol extract and the mixing of described Rabdosia rubescens water extract, obtain Rabdosia rubescens extract;
Obtain acceptable auxiliary agent on cosmeceutical;
Get acceptable auxiliary agent mixing on described Rabdosia rubescens extract and described cosmeceutical, to obtain final product;
Described skin care item are cosmetic water; On described cosmeceutical, acceptable auxiliary agent is bean sprout extract, Fructus Cucumidis sativi extract, oligochitosan, collagen protein, moisture-regulating agent, wetting emulsifier and water;
The mass ratio of described Rabdosia rubescens extract, described bean sprout extract, described Fructus Cucumidis sativi extract, described oligochitosan, described collagen protein, described moisture-regulating agent, described wetting emulsifier and described water is (1 ~ 5): (5 ~ 30): (4 ~ 40): (0.5 ~ 2): (0.2 ~ 3): (10 ~ 30): (6 ~ 25): (20 ~ 80).
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| CN104473851B (en) * | 2014-12-26 | 2017-04-12 | 武汉健民大鹏药业有限公司 | Lip cosmetics and preparation method thereof |
| CN105534870A (en) * | 2016-02-01 | 2016-05-04 | 佛山市聚成生化技术研发有限公司 | Fish collagen moisturizer and preparation method of fish collagen moisturizer |
| CN106074687A (en) * | 2016-07-31 | 2016-11-09 | 徐漫 | A kind of Rabdosia rubescens is fresh squeezes vacuum drying powder decoction pieces and preparation technology thereof |
| CN107080784A (en) * | 2017-03-31 | 2017-08-22 | 肖志远 | Chinese medicine acne eliminating cream |
| CN108309868A (en) * | 2018-05-02 | 2018-07-24 | 广州雷诺生物科技有限公司 | A kind of air cushion BB frosts containing fullerene |
| CN108926525B (en) * | 2018-09-14 | 2021-08-31 | 南方医科大学 | External transdermal absorption preparation for arthritis |
| CN111939112A (en) * | 2020-09-15 | 2020-11-17 | 河南省济源市济世药业有限公司 | Rabdosia rubescens whitening antioxidant composition, preparation method thereof and rabdosia rubescens whitening antioxidant mask |
| CN112603863B (en) * | 2020-12-21 | 2022-12-13 | 河南济世药业有限公司 | Rabdosia rubescens low-boiling-point volatile matter, extraction method and application thereof, and mosquito repelling product |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| Title |
|---|
| 迷迭香酸的研究概况;陈立亚;《中国药事》;20071231;第21卷(第11期);923-926、929 * |
| 迷迭香酸的研究进展;吴建章等;《天然产物研究与开发》;20051231;第17卷(第3期);383-388 * |
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