CN103570448A - Large-scale innocent treatment technique of tobacco stems - Google Patents
Large-scale innocent treatment technique of tobacco stems Download PDFInfo
- Publication number
- CN103570448A CN103570448A CN201210282091.3A CN201210282091A CN103570448A CN 103570448 A CN103570448 A CN 103570448A CN 201210282091 A CN201210282091 A CN 201210282091A CN 103570448 A CN103570448 A CN 103570448A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- tobacco rod
- tobacco
- temperature
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention provides a large-scale innocent treatment technique of tobacco stems. The technique comprises the steps of collection of tobacco stems, crushing, high-temperature and high-pressure separation treatment and fermentation, wherein the specific process of the fermentation step comprises the steps: mixing tobacco stems, nutrition source substances and accessories at a mass ratio of (70-75):(10-20):(10-20), adjusting the moisture content to 50-60% and adding an AT fermentation medium accounting for 0.05-0.1% of the total amount of fermentation materials. The materials thoroughly decomposed by the technique is used as a functional fertilizer and a seedling substrate; the AT fermentation medium can overcome influence of nicotine in inapplicable tobacco leaves and realize quick temperature rise and decomposition; a high temperature above 60 DEG C is generated in a decomposition process and lasts for more than one week, so pathogenic bacteria, worm eggs, weed seeds and the like can be effectively killed; innocent treatment is performed on waste tobacco stems (leaves and stems) through a high-temperature and high-pressure separator, so viruses are thoroughly killed and the structure of the tobacco stems can be changed and thus the cellulose and lignin in the tobacco stems can be separated out; large molecular substances in organic matters are fast transformed into small molecular substances such as small molecular sugars and amino acids which can be directly absorbed and used by tobaccos; the small molecular substances is used for producing a functional organic fertilizer or used as a seedling substrate. After being returned to field, the fertilizer can achieve the effects of reducing the volume weight of soil, promoting the formation of a soil aggregate structure, improving the activity of soil microorganism and the potassium content in the soil, effectively preventing soil-borne diseases, improving the tobacco yield, improving the tobacco quality and the like.
Description
Technical field
The invention belongs to tobacco rod processing technology field, be specifically related to a kind of tobacco rod mass-producing, innoxious process for treating.
Background technology
Traditional agriculture is faced with the problems such as environmental pollution, ecological damage, resource exhaustion.Therefore, by developing a circular economy, explore a kind of high-quality, the novel agricultural mode of production efficient, capable of circulation, form the production model of " resource → agricultural-food → renewable resource → agricultural-food ", become the vital task of modern agricultural development.
Tobacco is one of leading industry of China's rural economy, and national tobacco planting area reaches more than 1,500 ten thousand mu.Tobacco growing needs a large amount of potassium, phosphoric, all needs every year to supplement potassium, phosphor resource in soil.And in the tobacco rods such as footing leaf, offal, fireworks, cigarette wooden fork and part top, contain the nutritive elements such as the potassium that is easy in a large number to absorb, phosphorus.So it is the important channel of realizing tobacco sustainable development that tobacco rod is processed.
Owing to containing nicotine etc. in tobacco leaf and be unfavorable for the composition of microorganism growth, so traditional HM decomposing agent can not well become thoroughly decomposed to tobacco leaf, in prior art, general treatment process is field emergency burial, easily causes viral superinfection.Some place is to build treating pond, waste resource.Owing to carrying a large amount of virus, germ and worm's ovum in tobacco rod.If harmless treatment is not thorough, these pathogenic bacterias will be taken back to soil, pollute.
Summary of the invention
The object of the invention is to solve the above-mentioned technical problem providing in prior art, a kind of tobacco rod mass-producing, innoxious process for treating are provided.
For achieving the above object, the technical solution used in the present invention is as follows:
Tobacco rod mass-producing of the present invention, innoxious process for treating, comprise the collection of tobacco rod, pulverize, High Temperature High Pressure separating treatment, fermentation step, the concrete technology of described fermentation step is: by tobacco rod, nutrition source material and auxiliary material are that the ratio of 70-75:10-20:10-20 is mixed in mass ratio, regulate moisture to 50-60%, add the AT fermentation base of 0.5-1 ‰ fermented product total amount, above-mentioned fermentation materials is sent in fermenter, maintain 60 ~ 75 ℃ of fermentations 7 ~ 10 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (45 1 55 ℃) ferments 2 times, 5-10 days fermentation ends
,finished product is dried, is packaged to be to fermentation materials.
Fermenter described in the present invention can adopt normal fermentation groove of the prior art, and preferably adopting the patent No. is 200620030881.2, and denomination of invention is the disclosed technical scheme of closed aerating fecal treatment apparatus, and this technology can prevent secondary pollution.
Bacterial classification formula and the mass percent thereof of described AT (obsolete tabacum) fermentation base are as follows: bacillus natto 5-50%, penicillium oxalicum (Penicillium oxalicun) HB09-4(Classification And Nomenclature: penicillium oxalicum, Latin name: Penicillium oxalicun, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution is called for short: CGMCC, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on July 17th, 2012, deposit number: 6321) 5-50%, bacillus megaterium 5-50%, heat common streptomycete (Streptomyces thermovulgaris) HD12-4 (Classification And Nomenclature: hot common streptomycete, Latin name: Streptomyces thermovulgaris, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution is called for short: CGMCC, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on July 17th, 2012, deposit number: 6322) 10-70%.
AT for the treatment of tobacco rod fermentation base of the present invention, screening formulation is as follows: bacillus natto 10-30%, penicillium oxalicum HB09-4 20-40%, bacillus megaterium 10-30%, hot common streptomycete HD12-4 20-40%.
In described High Temperature High Pressure separating treatment technique, separating tank pressure is 1.9-2.0MPa, and temperature is 211-213 ℃
continue 1-2 minute.
Described nutrition source is chicken manure or rapeseed meal or both mixtures.
Described auxiliary material is Semen Maydis powder or wheat bran or both mixtures.
The preparation technology of the fermentation of the AT for the treatment of tobacco rod base of the present invention, comprises the steps:
(1) triangular flask liquid culture: fill 200ml meat soup liquid nutrient medium in 500ml triangular flask, access respectively bacillus megaterium and bacillus natto in 2 ring slant tubes after sterilizing, 32 degree shaking tables are cultivated 15 hours, carry out tank body and expand numerous; In 500ml triangular flask, fill 200mlPDA liquid nutrient medium, access respectively bacillus natto and penicillium oxalicum HB09-4 in 2 ring slant tubes after sterilizing, 25-30 degree shaking table is cultivated 17-18 hour, carries out tank body and expands numerous; In 500ml triangular flask, fill 200ml Gause I liquid nutrient medium, access respectively the common streptomycete HD12-4 of heat in 2 ring slant tubes after sterilizing, 50-55 degree shaking table is cultivated 17-18 hour, carries out tank body and expands numerous;
(2) solid culture: will adsorb in the sterilized solid carrier wheat bran 100% of the tank body liquid inoculation of bacillus megaterium and bacillus natto, the ratio of liquid and solid is 1:2.4, then dries; Bacillus natto and penicillium oxalicum tank body nutrient solution are inoculated into respectively to solid medium dregs of beans: 13%, Semen Maydis powder: 10%, wheat bran: 60%, fine flour: in 12%, inoculum size is 7.5%, after fully stirring evenly, is placed in the pallet of lower curtate Reticular breathable, cultivates 3 days under the condition of 30 degree; The common streptomycete tank body of heat nutrient solution is decomposed and is inoculated into solid medium dregs of beans: 15%, Semen Maydis powder: 35%, wheat bran: 45%, sawdust: in 5%, inoculum size is 7.5%, after fully stirring evenly, be placed in sterilized aluminum bucket, it is to increase Air permenbility that there is aperture at the bucket end, cultivates 2 days under the condition of 50-55 degree;
(3) composite: above cultured solid fungicide to be dried or dry aseptic ventilation, according to bacillus natto 5-50, penicillium oxalicum 5-50, bacillus megaterium 5-50, heat common streptomycete 10-70 ratio mix, the bacterial content of solid fermentation substrate be 4-6 hundred million/gram.
The penicillium oxalicum HB09-4 the present invention relates to carries out preservation in July, 2012 17 China Committee for Culture Collection of Microorganisms common micro-organisms center, Classification And Nomenclature: penicillium oxalicum, Latin name: Penicillium oxalicun, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution is called for short: CGMCC, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on July 17th, 2012, deposit number: 6321.
Colonial morphology and microscopic features are as follows:
Bacterial classification is grown comparatively fast on malt extract medium, lower 4 days of 25 ℃ of dark conditions, and colony diameter 19-21mm, quality is velvet-like, grayish green, sprawls, and conidial fructification forms in a large number; The bacterium colony back side is light brown, without water colo(u)r.
Conidiophore is tall and big, and wall is smooth, and penicillus 2 is verticillate, arrange closely, and bottle stalk column, neck is short, 8.2-13.6*2.5-3.5 m; Conidium is oval, light green, and wall is smooth, 3.5-6.9*2.0-3.3 m.There are no condition, produce robe structure.
The common streptomycete HD12-4 of heat the present invention relates to carries out preservation in July, 2012 17 China Committee for Culture Collection of Microorganisms common micro-organisms center, Classification And Nomenclature: hot common streptomycete, Latin name: Streptomyces thermovulgaris, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution is called for short: CGMCC, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on July 17th, 2012, deposit number: 6322.
Form and physicochemical characteristics are as follows:
(1) cultural characteristic
| Cultural characteristic | Aerial hyphae | Substrate mycelium | Soluble pigment |
| Czapek's solution | Beige | Shallow drabon look | Nothing |
| Glucose asparagine substratum | White | Milky white | Nothing |
| Glycerine asparagine substratum | White | Milky white | Nothing |
| Inorganic salt starch culture-medium | Beige | Drabon look | Nothing |
| ISP-2 substratum | Seldom | Shallow khaki color | Nothing |
| Oatmeal substratum | Seldom | Milky white | Nothing |
| Gause I substratum | White | Milky white | Nothing |
| Mulberry Ta Shi substratum | Seldom | Khaki color | Nothing |
(2) microscopic features: fibrillae of spores is straight, flexible, hook-shaped, volution, flexible are straight.Spore is oval.
(3) physiological and biochemical property:
The bacillus natto the present invention relates to is bacillus natto HA08-1(, is purchased from Institute of Microorganism, Academia Sinica; The bacillus megaterium the present invention relates to is bacillus megaterium HA09-6, is purchased from Institute of Microorganism, Academia Sinica.
AT fermentation base of the present invention can overcome the impact of nicotine in inapplicable tobacco leaf, realization is rapidly heated and becomes thoroughly decomposed, in digest process, produce the above high temperature of 60 degree, and continue more than one week, can effectively kill pathogenic bacteria, worm's ovum, weed seed etc., through high temperature, high-pressure separator is to discarded cigarette stalk (leaf, stalk) carry out harmless treatment, thorough kill virus change its structure and make Mierocrystalline cellulose, xylogen obtains separation, make the macromolecular substance in organism be converted into rapidly the small-molecule substance that tobacco can directly absorb, as small molecular sugar, amino acid etc., produce functional fertilizer or use as seedling medium.This fertilizer is also behind field, can play to reduce the soil weight, promote to form soil aggregate, improve soil microbial activities, improve soil potassium content, effectively prevent that soil-borne disease from tobacco production occurring, improving, and promotes the effects such as cigarette quality.
In the front inapplicable tobacco leaf of autoclave sterilization processing, bacterial content sum is every gram 1,2,300,000,000, and autoclave sterilization is processed rear bacterial content sum and is down to every gram 0,00,043 hundred million, substantially reaches aseptic.
The material that becomes thoroughly decomposed after the present invention processes is as follows as the test-results of seedling medium.
Test group I: the material that becomes thoroughly decomposed in booth, the embodiment of the present invention 1 being obtained builds up seedbed, in March, 2011 sowing, test plant is cotton, the non-soil culture carrying out management, regularly waters and executes nutritive medium and promoter (liquid).After one month, investigate its growth indexes (two leaf stage) and transplant about 0 day in Table Isosorbide-5-Nitrae, transplant and within latter 10 days, investigate its index of correlation (in Table 2).
Test group II: the material that becomes thoroughly decomposed in booth, the embodiment of the present invention 2 being obtained builds up seedbed, and management process is with test group I.
Test group III: the material that becomes thoroughly decomposed in booth, the embodiment of the present invention 3 being obtained builds up seedbed, and management process is with test group I.
Control group: get soil in booth and be designed to control group as seedbed, management process is with test group I.
Table 1 the present invention material that becomes thoroughly decomposed is educated the cotton seedling index of cotton seedling two leaf stage
| Project | Plant height (cm) | True leaf number (sheet/strain) | Stem thick (cm) | Root fresh weight (g/ strain) | Fresh weight (g/ strain) |
| Test group I | 3.1 | 2.3 | 0.21 | 0.36 | 1.70 |
| Test group II | 3.2 | 2.2 | 0.23 | 0.37 | 1.68 |
| Test group III | 3.2 | 2.2 | 0.22 | 0.38 | 1.69 |
| Control group | 3.1 | 2.0 | 0.19 | 0.30 | 1.43 |
Table 2 the present invention material that becomes thoroughly decomposed is educated cotton seedling growing state comparison after cotton transplantation of seedlings
| Project | Plant height | Main root long (cm) | Length is greater than the lateral root number (bar/strain) of 0.5cm | Newborn white root (bar/strain) | Complete stool fresh weight | Transplanting survival rate (%) |
| Test group I | 3.2 | 8.2 | 30.8 | 20.6 | 1.98 | 96.3 |
| Test group II | 3.2 | 8.1 | 32.1 | 19.8 | 1.88 | 94.6 |
| Test group III | 3.3 | 8.3 | 33.2 | 22.4 | 1.96 | 95.3 |
| Control group | 3.1 | 6.5 | 10.8 | 6.9 | 1.32 | 80.6 |
As can be seen from the above table, the inventive method gained material that must become thoroughly decomposed, cotton seedling growing state is obviously better than soil matrix.
The material that becomes thoroughly decomposed after the present invention processes is as follows as the field test results of fertilizers.
Field test one, the impact of function fertilizer of the present invention on the preventive effect of tobacco bacterial wilt and cigarette strain growth, test-results is in Table 1.
Test site: margin administrative village, lake, Hao Gang township, Shangshui County, Zhoukou City, Henan Province, this ground stops safflower tobacco 5 years continuously, this peasant family is accustomed to applying as fertilizer with animal excrement and forage, stalk mixture every year before tobacco leaf is transplanted, within 2008, start to occur bacterial wilt, within 2010, bacterial wilt sickness rate is up to 15.8%.
Test method:
Test group I: test period is the 2-9 month in 2011, for 0.5 mu of examination area, tobacco leaf cultivation distance between rows and hills is 120 * 50cm, for examination fertilizer, is the material that becomes thoroughly decomposed of embodiment 1, fertilizing method is all determined according to local fertilising custom.
Test group II: test period is the 2-9 month in 2011, for 0.5 mu of examination area, tobacco leaf cultivation distance between rows and hills is 120 * 50cm, for examination fertilizer, is the material that becomes thoroughly decomposed of embodiment 2, fertilizing method is all determined according to local fertilising custom.
Test group III: test period is the 2-9 month in 2011, for 0.5 mu of examination area, tobacco leaf cultivation distance between rows and hills is 120 * 50cm, for examination fertilizer, is the material that becomes thoroughly decomposed of embodiment 3, fertilizing method is all determined according to local fertilising custom.
Control group: test period is the 2-9 month in 2011, for 0.5 mu of examination area, tobacco leaf cultivation distance between rows and hills is 120 * 50cm, the fertilizer of selecting for local fertilising custom for examination fertilizer, fertilizing method is all determined according to local fertilising custom.
Experimental group and control group in transplanting latter 30 days respectively, record diseased plant number when plucking for the first time tobacco leaf and plucking tobacco leaf second from the bottom time.
Table 1 impact of material on the preventive effect of tobacco bacterial wilt and cigarette strain growth of becoming thoroughly decomposed of the present invention
As can be seen from the above table, multi-Functional Fertilizers of the present invention has good prevention effect to soil-borne disease, can promote tobacco growing.
Field test two, the impact of function fertilizer of the present invention on fruit-vegetable quality, test-results is in Table 2.
Test site: Southern Pass village, Chao Ge town, Hebi City Qixian County, Henan Province
Test method:
Tomato group: test period is the 3-8 month in 2011, is the material that becomes thoroughly decomposed of embodiment 4 for examination fertilizer, and for 0.5 mu of examination area, fertilizing method is all determined according to local fertilising custom.
Control group: test period is the 3-8 month in 2011, the fertilizer of selecting for local fertilising custom for examination fertilizer, fertilizing method is all determined according to local fertilising custom.
Watermelon group: test period is the 3-8 month in 2011, is the material that becomes thoroughly decomposed of embodiment 5 for examination fertilizer, and for 0.5 mu of examination area, fertilizing method is all determined according to local fertilising custom.
Control group: test period is the 3-8 month in 2011, the fertilizer of selecting for local fertilising custom for examination fertilizer, fertilizing method is all determined according to local fertilising custom.
Grape group: test period is in December, 2010-2011 year October, is the material that becomes thoroughly decomposed of embodiment 6 for examination fertilizer, and for 0.5 mu of examination area, fertilizing method is all according to local fertilising custom and determine.
Control group: test period is in December, 2010-2011 year October, the fertilizer of selecting for local fertilising custom for examination fertilizer, fertilizing method is all according to local fertilising custom and determine.
Table 1 the affect result of material on fruit-vegetable quality of becoming thoroughly decomposed
Total sugar content rate of increase in statistics fruit, during Vc content rate of increase, adopts 10 groups of random sampling in fruit, then calculates the method for mean value.
As can be seen from the above table, the material that becomes thoroughly decomposed of the present invention can significantly improve the quality of products, and improves the quality of product.
Accompanying drawing explanation
Fig. 1 is the structural representation of the tripping device used in the present invention.
Embodiment
High Temperature High Pressure separating treatment in the present invention refers to tobacco rod High Temperature High Pressure tripping device carries out separating treatment, and described tobacco rod High Temperature High Pressure tripping device as shown in Figure 1, comprises high-duty boiler 1 and separating tank 4,6.16 meters of separating tank height, 1 meter of diameter.On described separating tank 4 tank body tops, be provided with the high pressure steam access mouth of pipe 3, the high pressure steam access mouth of pipe 3 is connected with high-duty boiler 1, at separating tank tank body top, be provided with charging bole 2, tank base is provided with discharge port 6, in tank base tank body, be provided with inverted cone pipe 5, cone pipe 5 tops and tank body are connected as a single entity, and bottom connects discharge port, and cone pipe is peripheral forms cavity structure with tank body.On tank body, be provided with pressure warning unit 7 and thermometer 8, can observe at any time tank internal pressure and temperature.
In order to facilitate the scene of high-duty boiler to install, the high pressure steam access mouth of pipe of described separating tank has three, and adjacent two high pressure steam access tube plane center of circle angles are 120 degree.
Its principle of work is as follows:
Tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, according to the difference of tobacco rod, adjust operating pressure and temperature.Take that to process discarded cigarette stalk be example, the material temperature in container is raised to 211 ℃ of left and right, vapor pressure reaches 1.9Mpa left and right,
continue 1-2 minuterear unlatching discharge port, under high temperature, high pressure, the xylogen in cigarette stalk, Mierocrystalline cellulose is separated.Because its action time is short, energy density is high and concentrated, and steam molecule can be penetrated between the macromole such as Mierocrystalline cellulose and xylogen, destroys plant tissue internal structure, thereby completes the separated of the tissues such as xylogen, Mierocrystalline cellulose and hemicellulose.
(1) collect, pulverize: the weave construction of tobacco rod is special, xylogen, content of cellulose are higher, directly become thoroughly decomposed more difficult; In addition discarded cigarette stalk, mostly with pathogenic bacteria and worm's ovum, first will be discarded tobacco rod and carry out closed collection, reduce the pollution of disease and pest to vega.Adopt pulverizer first tobacco rod to be ground into 2-3 centimetre of segment.
(2) High Temperature High Pressure separating treatment: tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, the material temperature in container is raised to 211-213 ℃, vapor pressure reaches 1.9-2.0Mpa,
continue 1-2 minutethe rear discharge port of opening rapidly completes high temperature the separated of mattress and cigarette stalk weave construction of going out.
(3) fermentation step, the ratio that through separated tobacco rod, nutrition source material and auxiliary material is in mass ratio 70-75:10-20:10-20 is mixed, regulate moisture to 50-60%, add the AT fermentation base of 0.5-1 ‰ fermented product total amount, above-mentioned fermentation materials is sent in fermenter, maintain 60 ~ 75 ℃ of fermentations 7 ~ 10 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (45 1 55 ℃) ferments, 5-10 days fermentation ends 2 times.
(4) material that becomes thoroughly decomposed of fermentation materials being dried.
Described nutrition source is chicken manure.
Described auxiliary material is Semen Maydis powder.
Bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: bacillus natto 20%, penicillium oxalicum HB09-4 30%, bacillus megaterium 20%, hot common streptomycete HD12-4 30%.
Its preparation technology is as follows:
(a) triangular flask liquid culture
In 500ml triangular flask, fill 200ml meat soup liquid nutrient medium, access respectively the bacillus natto of the bacillus megaterium in 2 ring slant tubes after sterilizing, 32 degree shaking tables are cultivated 15 hours, carry out tank body and expand numerous.
In 500ml triangular flask, fill 200mlPDA liquid nutrient medium, access respectively bacillus natto and penicillium oxalicum in 2 ring slant tubes after sterilizing, 25-30 degree shaking table is cultivated 17-18 hour, carries out tank body and expands numerous.
In 500ml triangular flask, fill 200ml Gause I liquid nutrient medium, access respectively the common streptomycete of heat in 2 ring slant tubes after sterilizing, 50-55 degree shaking table is cultivated 17-18 hour, carries out tank body and expands numerous.
(b) solid culture
To in the sterilized solid carrier wheat bran 100% of bacillus megaterium tank body liquid inoculation, adsorb, the ratio of liquid and solid is 1:2.4, then dries.
Bacillus natto and the decomposition of penicillium oxalicum tank body nutrient solution are inoculated into solid medium dregs of beans: 13%, Semen Maydis powder: 10%, wheat bran: 60%, fine flour: in 12%, inoculum size is 7.5%, after fully stirring evenly, is placed in the pallet of lower curtate Reticular breathable, cultivates 3 days under the condition of 30 degree.
The common streptomycete tank body of heat nutrient solution is decomposed and is inoculated into solid medium dregs of beans: 15%, Semen Maydis powder: 35%, wheat bran: 45%, sawdust: in 5%, inoculum size is 7.5%, after fully stirring evenly, be placed in sterilized aluminum bucket, it is to increase Air permenbility that there is aperture at the bucket end, cultivates 2 days under the condition of 50-55 degree;
(c) composite: above cultured solid fungicide to be dried or dry aseptic ventilation, mass ratio is according to bacillus natto 20%, penicillium oxalicum 30%, bacillus megaterium 20%, heat common streptomycete 30% ratio mix, the bacterial content of solid fermentation substrate be 4-6 hundred million/gram.
(1) collect, pulverize: the weave construction of tobacco rod is special, xylogen, content of cellulose are higher, directly become thoroughly decomposed more difficult; In addition discarded cigarette stalk, mostly with pathogenic bacteria and worm's ovum, first will be discarded tobacco rod and carry out closed collection, reduce the pollution of disease and pest to vega.Adopt pulverizer first tobacco rod to be ground into 2-3 centimetre of segment.
(2) High Temperature High Pressure separating treatment: tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, the material temperature in container is raised to 213 ℃, vapor pressure reaches 2.0Mpa,
continue 1 minutethe rear discharge port of opening rapidly completes high temperature the separated of mattress and cigarette stalk weave construction of going out.
(3) fermentation step, the ratio that is 70:20:10 in mass ratio by inapplicable tobacco leaf, nutrition source material and auxiliary material through separated is mixed, regulate moisture to 50%, add the AT fermentation base of 0.5 ‰ fermented product total amounts, above-mentioned fermentation materials is sent in fermenter, maintain 60 ℃ of fermentations 10 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (55 ℃) ferments, 5 days fermentation ends 2 times.
(4) fermentation materials is dried and obtained the material that becomes thoroughly decomposed.
Described nutrition source is rapeseed meal.
Described auxiliary material is wheat bran.
Bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: bacillus natto 20%, penicillium oxalicum HB09-4 20%, bacillus megaterium 20%, hot common streptomycete HD12-4 40%.
Its preparation technology is with embodiment 1.
(1) collect, pulverize: the weave construction of tobacco rod is special, xylogen, content of cellulose are higher, directly become thoroughly decomposed more difficult; In addition discarded cigarette stalk, mostly with pathogenic bacteria and worm's ovum, first will be discarded tobacco rod and carry out closed collection, reduce the pollution of disease and pest to vega.Adopt pulverizer first tobacco rod to be ground into 2-3 centimetre of segment.
(2) High Temperature High Pressure separating treatment: tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, the material temperature in container is raised to 211 ℃, vapor pressure reaches 1.9Mpa,
continue 2 minutesthe rear discharge port of opening rapidly completes high temperature the separated of mattress and cigarette stalk weave construction of going out.
(3) fermentation step, the ratio that through separated tobacco rod, nutrition source material and auxiliary material is in mass ratio 75:15:10 is mixed, regulate moisture to 55%, add the AT fermentation base of 0.8 ‰ fermented product total amounts, above-mentioned fermentation materials is sent in fermenter, maintain 75 ℃ of fermentations 7 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (45 ℃) ferments, 10 days fermentation ends 2 times.
(4) fermentation materials is dried, is packaged to be finished product.
Described nutrition source is the mixture of chicken manure and rapeseed meal, and both mass ratioes are 1:1.
Described auxiliary material is the mixture of Semen Maydis powder and wheat bran, and both mass ratioes are 2:1.
Bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: bacillus natto 20%, penicillium oxalicum HB09-4 20%, bacillus megaterium 10%, hot common streptomycete HD12-4 50%.
Its preparation technology is with embodiment 1.
(1) collect, pulverize: the weave construction of tobacco rod is special, xylogen, content of cellulose are higher, directly become thoroughly decomposed more difficult; In addition discarded cigarette stalk, mostly with pathogenic bacteria and worm's ovum, first will be discarded tobacco rod and carry out closed collection, reduce the pollution of disease and pest to vega.Adopt pulverizer first tobacco rod to be ground into 2-3 centimetre of segment.
(2) High Temperature High Pressure separating treatment: tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, the material temperature in container is raised to 212 ℃, vapor pressure reaches 1.9Mpa,
continue 1.5 minutesthe rear discharge port of opening rapidly completes high temperature the separated of mattress and cigarette stalk weave construction of going out.
(3) fermentation step, the ratio that through separated tobacco rod, nutrition source material and auxiliary material is in mass ratio 75:15:10 is mixed, regulate moisture to 60%, add the AT fermentation base of 1 ‰ fermented product total amounts, above-mentioned fermentation materials is sent in fermenter, maintain 70 ℃ of fermentations 8 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (43 ℃) ferments, 8 days fermentation ends 2 times.
(4) fermentation materials is dried and is obtained the material that becomes thoroughly decomposed.
Described nutrition source is the mixture of chicken manure and rapeseed meal, and both mass ratioes are 5:1.
Described auxiliary material is the mixture of Semen Maydis powder and wheat bran, and both mass ratioes are 1:5.
Bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: bacillus natto 10%, penicillium oxalicum HB09-4 10%, bacillus megaterium 10%, hot common streptomycete HD12-4 70%.
Its preparation technology is with embodiment 1.
(1) collect, pulverize: the weave construction of tobacco rod is special, xylogen, content of cellulose are higher, directly become thoroughly decomposed more difficult; Discarded cigarette stalk, mostly with pathogenic bacteria and worm's ovum, first will be discarded tobacco rod and carry out respectively closed collection, reduce the pollution of disease and pest to vega.Adopt pulverizer first tobacco rod to be ground into 2-3 centimetre of segment.
(2) High Temperature High Pressure separating treatment: tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, the material temperature in container is raised to 211-213 ℃, vapor pressure reaches 1.9-2.0Mpa,
continue 2 minutesthe rear discharge port of opening rapidly completes high temperature the separated of mattress and cigarette stalk weave construction of going out.
(3) fermentation step, the ratio that is 75:10:15 in mass ratio by the tobacco rod after separation, nutrition source material and auxiliary material is mixed, regulate moisture to 56%, add the AT fermentation base of 0.9 ‰ fermented product total amounts, above-mentioned fermentation materials is sent in fermenter, maintain 72 ℃ of fermentations 9 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (52 ℃) ferments, 7 days fermentation ends 2 times.
(4) fermentation materials is dried, is packaged to be finished product.
Described nutrition source is the mixture of chicken manure and rapeseed meal, and both mass ratioes are 2:1.
Described auxiliary material is the mixture of Semen Maydis powder and wheat bran, and both mass ratioes are 1:3.
Bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: bacillus natto 10%, penicillium oxalicum HB09-4 20%, bacillus megaterium 50%, hot common streptomycete HD12-4 20%.
Its preparation technology is with embodiment 1.
(1) collect, pulverize: the weave construction of tobacco rod is special, xylogen, content of cellulose are higher, directly become thoroughly decomposed more difficult; Discarded cigarette stalk, mostly with pathogenic bacteria and worm's ovum, first will be discarded tobacco rod and carry out closed collection, reduce the pollution of disease and pest to vega.Adopt pulverizer first tobacco rod to be ground into 2-3 centimetre of segment.
(2) High Temperature High Pressure separating treatment: tobacco rod, after collecting, pulverizing, is transported to separating tank charging bole with lift, arrives separating tank inner, the material temperature in container is raised to 211-213 ℃, vapor pressure reaches 1.9-2.0Mpa,
continue 2 minutesthe rear discharge port of opening rapidly completes high temperature the separated of mattress and cigarette stalk weave construction of going out.
(3) fermentation step, the ratio that through separated tobacco rod, nutrition source material and auxiliary material is in mass ratio 75:20:15 is mixed, regulate moisture to 58%, add the AT fermentation base of 0.6 ‰ fermented product total amounts, above-mentioned fermentation materials is sent in fermenter, maintain 68 ℃ of fermentations 10 days, stir turning every day and as temperature drops to 45 ℃, just stir below turning 1 time (do not decline and also will stir turning on time) later for middle low temperature (50 ℃) ferments, 8 days fermentation ends 2 times.
(4) fermentation materials is dried, is packaged to be finished product.
Described nutrition source is chicken manure.
Described auxiliary material is Semen Maydis powder.
Bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: mass ratio is according to bacillus natto 50%, penicillium oxalicum 15%, bacillus megaterium 15%, the ratio of hot common streptomycete 20% mixes, the bacterial content of solid fermentation substrate be 4-6 hundred million/gram.
Its preparation technology is with embodiment 1.
The effect that AT fermentation base becomes thoroughly decomposed to tobacco rod high temperature:
1. experiment purpose
By different treatment to become thoroughly decomposed in the rotten test of tobacco rod (cigarette stalk, inapplicable tobacco leaf) heap temperature, total N, P, K; C/N, Lignin degradation rate, dynamic change and the contrast on the impact of tobacco rod quality of compost products thereof of AT fermentation base (penicillium oxalicum, hot common streptomycete) on the adaptability of nicotine, seed germination index (GI); illustrate that processing has relative advantage to AT fermentation base to tobacco rod, processes foundation is provided for mass-producing.
2. experiment material
2.1 tobacco rods: tobacco leaf is directly pulverized, cigarette stalk is pulverized and separating treatment
The physical and chemical index of tobacco rod: organism: 78.24%, the about 45-50% of content of lignin, N:1.11% P:0.58% K:1.64% nicotine (Nicotine) popular name Nicotine, tobacco leaf content: be about 1-3%, cigarette stalk content is about 0.3-1.1%, because tobacco bred is different, has any different.
2.2 nutritional supplements: the wheat bran that mass ratio is 1:1 and the mixture of Semen Maydis powder
The AT fermentation base of 2.3 embodiment of the present invention 1
2.4 general decomposing microbial inoculums: adopt the disclosed decomposing microbial inoculum of Chinese patent CN101838613A.
3. experimental technique
3.1 experiments are processed
| Process | Combination |
| Process one | Tobacco rod (70%)+nutritional supplements (30%) |
| Process two | Tobacco rod (70%)+nutritional supplements (30%)+general decomposing microbial inoculum (1 ‰) |
| Process three | Tobacco rod (70%)+nutritional supplements (30%)+AT base (1 ‰) that ferments |
3.2 compost method
Fermented product moisture 50-60%, heap is long not to be limit, and piles wide 1.5m, piles high 0.8m
3.3 research object
Temperature, total N, P, K, C/N, Lignin degradation rate, rate of emergence, the adaptability of AT fermentation base (penicillium oxalicum, hot common streptomycete) to nicotine.
experimental result and analysis
Result shows, add AT fermentation base and shortened the time that tobacco rod compost reaches a high temperature, extended the pyrolytic decomposition time length, increase total nitrogen content, accelerate the degradation rate of material N, P, K and C/N ratio, improve seed germination index (GI), AT fermentation base (penicillium oxalicum, hot common streptomycete) has affinity, adaptability to cigarette stalk and the nicotine being not suitable with in tobacco leaf, can decompose tobacco rod.Accelerated tobacco rod compost maturity process.
4.1 AT fermentation bases are affecting tobacco rod leavening temperature
While processing a 24h, temperature is 35 ℃, and top temperature is 48 ℃; While processing two 24h, temperature is 45 ℃, enters the pyrolytic decomposition stage during 3d, and top temperature is 56 ℃, high-temperature duration 3d; While processing three 24h, temperature is 55 ℃, and entered the pyrolytic decomposition stage same day, and top temperature reaches 68 ℃, high-temperature duration 7-10d.Process three process two in advance 2d enter the pyrolytic decomposition stage, high-temperature duration extends respectively 4-6d, and processes three top temperatures apparently higher than processing two and process one, exceeds respectively 12 ℃, 20 ℃.
Research discovery, lignocellulose is mainly decomposed at hot stage, and the high temperature bacteria strain that AT ferments in base accelerates heap body and heats up, the decomposition of lignocellulose material in quickening compost substrate, thereby the cycle of shortening compost maturity.
4.2 AT fermentation bases are affecting tobacco rod quality
It is as follows that three kinds of processing are surveyed respectively its nutrient composition content after fully becoming thoroughly decomposed:
| Process | N（%） | P（%） | K（%） |
| Process one | 0.99 | 1.58 | 2.64 |
| Process two | 1.09 | 2.08 | 3.15 |
| Process three | 1.11 | 2.83 | 4.33 |
The processing of adding AT fermentation base microbial inoculum can significantly increase total N, P, the K nutrient content of tobacco rod composting production.Processing two increases by 10.1% than processing a total N, and total P increases by 31.6%, and total K increases by 19.3%; Processing three increases by 1.8% than processing two total N, and total P increases by 36.1%, and total K increases by 37.5%.
In tobacco rod, the nutritive element content such as P, K is higher, but how to form mixture with organic matter, can not be used effectively, and have the bacterial classification of special decomposition organophosphorus, organic potassium, thereby in promotion tobacco rod, P, K composition decomposes release in AT fermentation base.
4.3?C/N
Compost needs suitable C/N, and one removes in compost and requires suitable C/N for (25-30): 1, and too low as C/N, can not provide enough energy substances for microorganism, too high as C/N, in composting process, aerobic requirement improves and produces a large amount of foul smell.The C/N of tobacco rod is higher by approximately 36, by nutritional supplements (wheat bran, Semen Maydis powder), makes amendment, regulates the C/N of compost to 25 left and right.
C/N be compost maturity an important indicator, when C/N reaches (15-20): in the time of 1, think compost maturity.
Compost maturity is to 3d, processes one, two, three C/N ratio and is respectively 28.7,27.1,26.8, processes one, two, three C/N ratio and be respectively 25.8,23.3,22.1 during 7d, processes three C/N and be down to 18.6 during 11d, reaches the standard of becoming thoroughly decomposed.As can be seen here, the tobacco heap ferment effect of interpolation AT fermentation base is obviously better.
4.4 impacts of AT fermentation base on lignin degradation efficiency
In cigarette stalk, degree of lignification is higher, the about 45-50% of content of lignin, and after wood fibre separating treatment, content of lignin is still in 30% left and right.And the general about 15-20% of Spruce lignin content, so the degraded of lignocellulose is the principal element of restriction cigarette stalk compost maturity.
Compost is during to 15d, now processes one, two, three content of lignin respectively 28.2%, 24.4%, 12.7%.Process an xylogen substantially without degraded, process two content and slightly reduce, process three degradation effects remarkable.
The bacterial strain that has efficient degradation lignocellulose in AT fermentation base, can produce extracellular lignocellulose lytic enzyme, active far away higher than enzyme in the spore of bacterium, accelerates rate of decomposition and the efficiency of lignocellulose material in compost substrate.
4.5 AT fermentation bases are affecting seed germination index (GI)
The phytotoxicity of measuring compost by biological method is the effective ways of check compost maturity, and seed germination index is innoxious, the important indicator of degree of stability.
To compost 7d, each germination index of processing seed is respectively: 28.8%, 58.2%, 82.1%, wherein the fastest to process three germination index lift velocity, to compost to 15d, each germination index of processing seed is respectively: 66.8%, 72%, 86%, process three and met the requirement of becoming thoroughly decomposed completely.Add AT fermentation base and can effectively improve seed germination index.
The adaptability of 4.6 AT fermentation bases (penicillium oxalicum, hot common streptomycete) to nicotine
Ferment base and common decomposing agent of AT made to the bacterium liquid of same equal proportion, be inoculated into respectively with in tobacco rod (cigarette stalk accounts for 70%, and inapplicable tobacco leaf accounts for 30%) matrix, observe their growing state.Found that, after inoculation 24h, in the tobacco rod matrix of AT fermentation base, start to occur white hypha, during to 48h, grown a large amount of white hyphas, and now inoculate in the tobacco rod matrix of common decomposing agent, only has a small amount of mycelia, subsequently, the tobacco rod matrix of inoculation AT fermentation base is covered with the mycelia approximately sustainable several days on vein surface, simultaneously generating portion spore. and the mycelia that inoculates the tobacco rod matrix of common decomposing agent does not significantly change.Add as can be seen here AT fermentation base (mould, strepto-) cigarette stalk and the nicotine being not suitable with in tobacco leaf are had to stronger affinity, adaptability.
experiment conclusion:
1. do not add in the rotten process of fermenting of tobacco rod heap of microbial inoculum, heat-up rate is slow, can not enter the pyrolytic decomposition stage, and seed germination index is lower, and becoming thoroughly decomposed process is slower, and the degree of becoming thoroughly decomposed is lower.
2, add the tobacco rod of removing decomposing microbial inoculum and pile in rotten fermenting process, heat-up rate is slower, and the time that enters the pyrolytic decomposition stage is longer, and high-temperature duration is shorter, and most microbial inoculums are had to restraining effect.
3. it is very fast that the tobacco rod that adds AT fermenting agent is piled in rotten process heat-up rate, heap body enters rapidly the pyrolytic decomposition stage, high-temperature duration extends, shorten the compost fermentation time, can promote C/N to reduce, significantly improve seed germination index, and can significantly improve the total nitrogen, total phosphorus of composting production, the content of total potassium, improve the quality of composting production.
Claims (5)
1. a tobacco rod mass-producing, innoxious process for treating, it is characterized in that: comprise the collection of tobacco rod, pulverize, High Temperature High Pressure separating treatment, fermentation step, the concrete technology of described fermentation step is: by tobacco rod, nutrition source material and auxiliary material are that the ratio of 70-75:10-20:10-20 is mixed in mass ratio, regulate moisture to 50-60%, add the AT fermentation base of 0.5-1 ‰ fermented product total amount, above-mentioned fermentation materials is sent in fermenter, maintain 60 ~ 75 ℃ of fermentations 7 ~ 10 days, stir turning every day 2 times, after temperature declines, at 45-55 ℃, carry out middle low temperature fermentation, 5-10 days fermentation ends, fermentation materials is dried to obtain to the tobacco leaf material that becomes thoroughly decomposed.
2. tobacco rod mass-producing according to claim 1, innoxious process for treating; it is characterized in that: bacterial classification formula and the mass percent thereof of described AT fermentation base are as follows: bacillus natto 5-50%; penicillium oxalicum HB09-4 5-50%; bacillus megaterium 5-50%, hot common streptomycete HD12-4 10-70%.
3. tobacco rod mass-producing according to claim 1, innoxious process for treating, is characterized in that: in described High Temperature High Pressure separating treatment technique, separating tank pressure is 1.9-2.0MPa, and temperature is 211-213 ℃ of lasting 1-2 minute.
4. tobacco rod mass-producing according to claim 1, innoxious process for treating, is characterized in that: described nutrition source is chicken manure or rapeseed meal or both mixtures.
5. tobacco rod mass-producing according to claim 1, innoxious process for treating, is characterized in that: described auxiliary material is Semen Maydis powder or wheat bran or both mixtures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210282091.3A CN103570448B (en) | 2012-08-09 | 2012-08-09 | Large-scale harmless treatment technique of tobacco stems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210282091.3A CN103570448B (en) | 2012-08-09 | 2012-08-09 | Large-scale harmless treatment technique of tobacco stems |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103570448A true CN103570448A (en) | 2014-02-12 |
| CN103570448B CN103570448B (en) | 2015-03-18 |
Family
ID=50043247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210282091.3A Expired - Fee Related CN103570448B (en) | 2012-08-09 | 2012-08-09 | Large-scale harmless treatment technique of tobacco stems |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103570448B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262032A (en) * | 2014-10-22 | 2015-01-07 | 中山市巴斯德农业科技有限公司 | Method for producing functional organic fertilizer by using trilobe wedelia |
| CN104557314A (en) * | 2015-01-19 | 2015-04-29 | 曹明军 | Multipurpose plant foliar nutrient solution and preparation method thereof |
| CN105567610A (en) * | 2016-03-03 | 2016-05-11 | 北京林业大学 | High-temperature-resistant garden waste decomposing bacteria ST5 and application thereof |
| CN107021804A (en) * | 2017-06-19 | 2017-08-08 | 安康市烟草公司汉滨烟叶分公司 | The culture medium prescription and preparation method and application of a kind of utilization tobacco rod culturing edible fungus |
| CN108476903A (en) * | 2018-02-09 | 2018-09-04 | 中国烟草总公司广东省公司 | One method to grow tobacco with rice rice field-upland field rotation and the reparation of mineral nutrition original place |
| CN108719032A (en) * | 2018-06-11 | 2018-11-02 | 云南绿漫农林科技发展有限公司 | A kind of breeding method of succulent |
| CN113277900A (en) * | 2021-06-29 | 2021-08-20 | 湖北中烟工业有限责任公司 | Production process of tobacco straw bio-organic fertilizer |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050103708A1 (en) * | 2002-02-07 | 2005-05-19 | Fetterman Lewis M. | Method for the treatment of animal waste and products made therefrom |
| CN1624100A (en) * | 2004-10-29 | 2005-06-08 | 南京师范大学 | Microbial fermenting agent |
| CN101613222A (en) * | 2009-07-17 | 2009-12-30 | 郑州大学 | A kind of tobacco stalk organic fertilizer and manufacturing thereof, using method |
| CN101624303A (en) * | 2009-08-11 | 2010-01-13 | 长沙浩博生物科技有限公司 | K-rich organic fertilizer and production method thereof |
| CN101734962A (en) * | 2009-12-25 | 2010-06-16 | 云南澄江立杉生物工程有限公司 | Tobacco powder biological organic fertilizer formula and production method thereof |
| CN102174412A (en) * | 2010-12-15 | 2011-09-07 | 云南大学 | Phosphorus-solubilizing fungi and application thereof to preparation of biological fungus fertilizer |
-
2012
- 2012-08-09 CN CN201210282091.3A patent/CN103570448B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050103708A1 (en) * | 2002-02-07 | 2005-05-19 | Fetterman Lewis M. | Method for the treatment of animal waste and products made therefrom |
| CN1624100A (en) * | 2004-10-29 | 2005-06-08 | 南京师范大学 | Microbial fermenting agent |
| CN101613222A (en) * | 2009-07-17 | 2009-12-30 | 郑州大学 | A kind of tobacco stalk organic fertilizer and manufacturing thereof, using method |
| CN101624303A (en) * | 2009-08-11 | 2010-01-13 | 长沙浩博生物科技有限公司 | K-rich organic fertilizer and production method thereof |
| CN101734962A (en) * | 2009-12-25 | 2010-06-16 | 云南澄江立杉生物工程有限公司 | Tobacco powder biological organic fertilizer formula and production method thereof |
| CN102174412A (en) * | 2010-12-15 | 2011-09-07 | 云南大学 | Phosphorus-solubilizing fungi and application thereof to preparation of biological fungus fertilizer |
Non-Patent Citations (1)
| Title |
|---|
| 徐凤花: "《微生物制品技术及应用》", 31 December 2007, 化学工业出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262032A (en) * | 2014-10-22 | 2015-01-07 | 中山市巴斯德农业科技有限公司 | Method for producing functional organic fertilizer by using trilobe wedelia |
| CN104557314A (en) * | 2015-01-19 | 2015-04-29 | 曹明军 | Multipurpose plant foliar nutrient solution and preparation method thereof |
| CN105567610A (en) * | 2016-03-03 | 2016-05-11 | 北京林业大学 | High-temperature-resistant garden waste decomposing bacteria ST5 and application thereof |
| CN105567610B (en) * | 2016-03-03 | 2019-09-13 | 北京林业大学 | One plant of high temperature resistant garden waste decomposer ST5 and its application |
| CN107021804A (en) * | 2017-06-19 | 2017-08-08 | 安康市烟草公司汉滨烟叶分公司 | The culture medium prescription and preparation method and application of a kind of utilization tobacco rod culturing edible fungus |
| CN108476903A (en) * | 2018-02-09 | 2018-09-04 | 中国烟草总公司广东省公司 | One method to grow tobacco with rice rice field-upland field rotation and the reparation of mineral nutrition original place |
| CN108719032A (en) * | 2018-06-11 | 2018-11-02 | 云南绿漫农林科技发展有限公司 | A kind of breeding method of succulent |
| CN113277900A (en) * | 2021-06-29 | 2021-08-20 | 湖北中烟工业有限责任公司 | Production process of tobacco straw bio-organic fertilizer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103570448B (en) | 2015-03-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103570446B (en) | Process technology for producing functional fertilizer by utilizing tobacco waste | |
| CN108624539B (en) | Straw and cow dung ultrahigh-temperature decomposition agent and preparation and application thereof | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN103570448B (en) | Large-scale harmless treatment technique of tobacco stems | |
| CN101165008B (en) | Vegetable residual strain microorganism-earthworm multiple step inoculation conversion method | |
| CN103980019A (en) | Agricultural solid organic waste medium temperature biological fermentation process and application thereof | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN101941851A (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| CN101113409B (en) | Method for cultivating antler mythic fungus by using bacterium glass | |
| CN108277177B (en) | Streptomyces microflavus solid fermentation medium, preparation method and fermentation method thereof, fermentation product, biocontrol product and application | |
| CN103626532B (en) | Fungicide containing bacillus and application thereof | |
| CN102826875B (en) | Microbial organic fertilizer and purpose of microbial organic fertilizer | |
| CN106244475A (en) | A kind of low temperature resistant compost fermentation microbial inoculum with deodorization functions and application thereof | |
| CN108823134A (en) | A kind of corn stover decomposing agent and preparation method thereof | |
| CN109006180A (en) | A kind of mushroom culture medium and preparation method thereof | |
| CN115336503A (en) | Preparation method of substitute bacterial material for gastrodia elata cultivation | |
| CN103570447B (en) | Tobacco waste is utilized to produce the method for seedling medium | |
| CN101928161B (en) | Method for preparing direct seeding compression nutrition pot raw material | |
| CN103980020B (en) | The biological organic fertilizer that a kind of decomposing microbial inoculum and natural organic matter material produce | |
| CN102603372B (en) | Simple production technology of special AM (arbuscular mycorrhiza) fungal manure for tobacco | |
| CN1414093A (en) | Microbial fertilizer bacterial agent for direct decomposing straw as fertilizer and its production process and application method | |
| CN102219567B (en) | Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation | |
| CN108383653A (en) | A kind of method and bio-organic fertilizer preparing fertilizer using stalk | |
| CN103564644B (en) | A kind of inapplicable tobacco leaf innoxious process for treating | |
| CN105624062A (en) | Ageratina adenophora organic fertilizer solid microbial inoculant and preparation method thereof, and application of microbial inoculant in organic fertilizer production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150318 Termination date: 20170809 |