CN103535528A - Preparation method of Kurobuta feed additives - Google Patents
Preparation method of Kurobuta feed additives Download PDFInfo
- Publication number
- CN103535528A CN103535528A CN201310510770.6A CN201310510770A CN103535528A CN 103535528 A CN103535528 A CN 103535528A CN 201310510770 A CN201310510770 A CN 201310510770A CN 103535528 A CN103535528 A CN 103535528A
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- CN
- China
- Prior art keywords
- bacillus subtilis
- preparation
- black
- subtilis black
- mutation
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- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000003674 animal food additive Substances 0.000 title abstract 5
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 20
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 20
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 241001627955 Tetraodon lineatus Species 0.000 claims description 22
- 230000035772 mutation Effects 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 10
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 abstract description 10
- 230000036039 immunity Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 235000013372 meat Nutrition 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000036564 melanin content Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to a preparation method of feed additives and in particular relates to a preparation method for Kurobuta feed additives. The preparation method comprises the following steps of cultivating a bacillus subtilis black variant culture solution, heating and concentrating the bacillus subtilis black variant culture solution to 50Bx, uniformly mixing the bacillus subtilis black variant culture solution with bran, wherein the masses of the bacillus subtilis black variant culture solution and the bran are equal; then drying the mixture until the water content is less than 10 percent, smashing the mixture, and screening to obtain a finished product through a sieve of 20 meshes. The feed additives prepared by the method contain melanin produced in a bacillus subtilis black variant growing and breeding process; if Kurobuta feeds on the feed additives, the disease resistance and the immunity can be improved; the content of melanin in the Kurobuta can be increased, so that the medicinal value and the edible value of the Kurobuta can be improved.
Description
Technical field
The present invention relates to the preparation method of feed addictive, particularly a kind of preparation method of black globefish feed addictive.
Background technology
Black globefish is mammality plant-eating animal, and its bodily form is little, medicine, eat dual-purpose.Black globefish delicious meat is delicate, absorption easy to digest, among the people being considered as " keep fit treasure ".Black globefish is a kind of plant-eating animal of small-sized grain-saving type, and its meat flavour is delicious, belongs to the meat of low fat, low cholesterol, has dense game characteristic.Black globefish meat contains rich in protein and containing various trace elements such as 17 seed amino acids and calcium, phosphorus, iron, and contains and enrich Anticancer Element Selenium zinc and selenium, also contains in addition a large amount of melanin in black globefish meat, can play effect anti-oxidant, that remove free radical.Therefore black globefish meat is a kind of desirable natural nourishing health products, has the effects such as nourishing, beauty treatment, establishing-Yang, to malnutrition, anaemia, insomnia, appetite are low, tired etc., all has a good regulating action.Often edible, required various nutriments in balanced body, promote normal secretion metabolism in body effectively, strengthen immunity.At present, the feeding quality of propagating black globefish artificially is uneven, causes cultivating functional nutritional labeling in black globefish body---and melanin declines than wild black globefish content, affects its be used as medicine effect and tonic effect.
Summary of the invention
The object of this invention is to provide a kind of preparation method safe, that increase the black globefish feed addictive of melanin content in black globefish body.
The object of the present invention is achieved like this:
(1) preparation of bacillus subtilis black mutation nutrient solution:
In clear water, add 2% glucose, 1% soluble starch, 1% corn protein powder, 0.05% yeast extract, 0.04% potassium dihydrogen phosphate, 0.02% magnesium sulfate, 0.01% calcium carbonate, 0.001% manganese sulfate, PH is adjusted to 7.0-7.2, at 121 ℃, sterilizing is 20 minutes, be cooled to 30 ℃, the mutation of inoculation bacillus subtilis black, under 29～31 ℃, the condition of rotary shaker 120rpm, cultivate 3 days, obtain bacillus subtilis black mutation nutrient solution.
(2) making of black globefish feed addictive:
Bacillus subtilis black mutation nutrient solution heating is concentrated into 50Bx, with etc. the wheat bran of quality mix, be then dried to moisturely below 10%, pulverized 20 mesh sieve and got product.
Feed addictive of the present invention, contain the melanin producing in bacillus subtilis black heterogonous growth reproductive process, after black globefish is edible, can not only improve premunition, strengthen immunity, can also increase the melanin content in its body, improve medical value and the edibility of black globefish.While feeding black globefish, every black globefish of adult 3-5 gram of feed addictive of the present invention of feeding every day, sneaks in black globefish daily ration and feeds.
(4), specific embodiments
The bacterial strain bacillus subtilis black mutation CICC20238 that the present invention adopts purchases in Chinese industrial microorganism fungus kind preservation administrative center.
(1) preparation of bacillus subtilis black mutation nutrient solution:
Take the glucose of 20g, the corn protein powder of the soluble starch of 10g, 10g, the potassium dihydrogen phosphate of the yeast extract of 0.5g, 0.4g, the calcium carbonate of the magnesium sulfate of 0.2g, 0.1g, the manganese sulfate of 0.01g, be settled to 1000ml, PH is adjusted to 7.0-7.2, at 121 ℃, sterilizing is 20 minutes, be cooled to 30 ℃, the mutation of inoculation bacillus subtilis black is cultivated 3 days under 30 ℃, the condition of rotary shaker 120rpm, obtains bacillus subtilis black mutation nutrient solution.
(2) making of black globefish feed addictive:
Bacillus subtilis black mutation nutrient solution heating is concentrated into 50Bx, with etc. the wheat bran of quality mix, be then dried to moisturely below 10%, pulverized 20 mesh sieve and got product.
Claims (2)
1. the preparation method of a black globefish feed addictive, it is characterized in that: first make bacillus subtilis black mutation nutrient solution, then the heating of bacillus subtilis black mutation nutrient solution is concentrated into 50Bx, with etc. the wheat bran of quality mix, then be dried to moisturely below 10%, pulverized 20 mesh sieve and get product.
2. the preparation method of a kind of black globefish feed addictive according to claim 1, it is characterized in that: the preparation method of described bacillus subtilis black mutation nutrient solution is: in clear water, add 2% glucose, 1% soluble starch, 1% corn protein powder, 0.05% yeast extract, 0.04% potassium dihydrogen phosphate, 0.02% magnesium sulfate, 0.01% calcium carbonate, 0.001% manganese sulfate, PH is adjusted to 7.0-7.2, at 121 ℃, sterilizing is 20 minutes, be cooled to 30 ℃, the mutation of inoculation bacillus subtilis black, at 29～31 ℃, under the condition of rotary shaker 120rpm, cultivate 3 days, obtain bacillus subtilis black mutation nutrient solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310510770.6A CN103535528B (en) | 2013-10-27 | 2013-10-27 | Preparation method of Kurobuta feed additives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310510770.6A CN103535528B (en) | 2013-10-27 | 2013-10-27 | Preparation method of Kurobuta feed additives |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103535528A true CN103535528A (en) | 2014-01-29 |
| CN103535528B CN103535528B (en) | 2015-01-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310510770.6A Active CN103535528B (en) | 2013-10-27 | 2013-10-27 | Preparation method of Kurobuta feed additives |
Country Status (1)
| Country | Link |
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| CN (1) | CN103535528B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104970256A (en) * | 2015-07-20 | 2015-10-14 | 北海市铁山港区富群养殖有限公司 | Preparation method of cavia porcllus feed |
| CN104996718A (en) * | 2015-07-20 | 2015-10-28 | 北海市铁山港区富群养殖有限公司 | Cavia porcllus pneumonia preventing and curing feed preparation method |
| CN105052822A (en) * | 2015-07-29 | 2015-11-18 | 北海市铁山港区富群养殖有限公司 | Breeding method of black guinea pig calf |
| CN108925501A (en) * | 2018-05-29 | 2018-12-04 | 凌云县郁丰农业专业合作社联合社 | A kind of cultural method of globefish leopard cat |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100910504B1 (en) * | 2008-10-08 | 2009-07-31 | 김대환 | The cultivation method of the high hardness and sugar content strawberry having the high content of vitamin and calcium |
| CN102726342A (en) * | 2012-07-19 | 2012-10-17 | 福建省农业科学院畜牧兽医研究所 | Ecological guinea pig breeding technique |
-
2013
- 2013-10-27 CN CN201310510770.6A patent/CN103535528B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100910504B1 (en) * | 2008-10-08 | 2009-07-31 | 김대환 | The cultivation method of the high hardness and sugar content strawberry having the high content of vitamin and calcium |
| CN102726342A (en) * | 2012-07-19 | 2012-10-17 | 福建省农业科学院畜牧兽医研究所 | Ecological guinea pig breeding technique |
Non-Patent Citations (3)
| Title |
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| 刘波，等: "枯草芽孢杆菌黑色变种ATCC 9372的特性及其应用", 《中国消毒学杂志》, vol. 26, no. 2, 31 December 2009 (2009-12-31) * |
| 李春凤，等: "枯草芽孢杆菌在饲料生产及环境防治中的应用", 《中国饲料》, no. 1, 5 January 2013 (2013-01-05) * |
| 薛志成: "中华黑豚的人工养殖技术", 《农村经济与科技》, no. 9, 31 December 2003 (2003-12-31), pages 37 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104970256A (en) * | 2015-07-20 | 2015-10-14 | 北海市铁山港区富群养殖有限公司 | Preparation method of cavia porcllus feed |
| CN104996718A (en) * | 2015-07-20 | 2015-10-28 | 北海市铁山港区富群养殖有限公司 | Cavia porcllus pneumonia preventing and curing feed preparation method |
| CN105052822A (en) * | 2015-07-29 | 2015-11-18 | 北海市铁山港区富群养殖有限公司 | Breeding method of black guinea pig calf |
| CN108925501A (en) * | 2018-05-29 | 2018-12-04 | 凌云县郁丰农业专业合作社联合社 | A kind of cultural method of globefish leopard cat |
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| Publication number | Publication date |
|---|---|
| CN103535528B (en) | 2015-01-14 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Guizhan Inventor before: Li Zhenlin Inventor before: Lv Weimin Inventor before: Li Na Inventor before: Yan Ying Inventor before: Ju Peihong Inventor before: Tan Yi |
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Effective date of registration: 20170718 Address after: 274418 Shandong city of Heze province Caoxian Gu Ying Ji Zhen Nan Tang Zhuang Xing Zheng Cun Lu Lou Cun 199 Patentee after: Li Guizhan Address before: 150010, 43, end street, Daoli District, Heilongjiang, Harbin Patentee before: Heilongjiang-Prov. Academy of Light Industry Sciences |
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Effective date of registration: 20180322 Address after: 536000 Beihai city jinhaian Road No. 45 the Guangxi Zhuang Autonomous Region North Bay technology innovation center 4 Building No. 0905 Patentee after: Beihai Hesi Technology Co., Ltd. Address before: 274418 Shandong city of Heze province Caoxian Gu Ying Ji Zhen Nan Tang Zhuang Xing Zheng Cun Lu Lou Cun 199 Patentee before: Li Guizhan |
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Effective date of registration: 20181203 Address after: 401120 No. 117 Songshi North Road, Yubei District, Chongqing Patentee after: Chongqing Hongbo Environmental Protection Engineering Co., Ltd. Address before: 536000 0905 tower 4, science and technology innovation center, Beibu Bay, 45 Golden Coast Road, Beihai, the Guangxi Zhuang Autonomous Region Patentee before: Beihai Hesi Technology Co., Ltd. |
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Effective date of registration: 20201015 Address after: 235000 Lantau Peak Industrial Park, Anhui, Huaibei Patentee after: ANHUI JISUSHIDAI ANIMAL NUTRITION Co.,Ltd. Address before: 401120 No. 117 Songshi North Road, Yubei District, Chongqing Patentee before: CHONGQING HONGBO ENVIRONMENTAL PROTECTION ENGINEERING Co.,Ltd. |