CN104970256A - Preparation method of cavia porcllus feed - Google Patents

Preparation method of cavia porcllus feed Download PDF

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Publication number
CN104970256A
CN104970256A CN201510427031.XA CN201510427031A CN104970256A CN 104970256 A CN104970256 A CN 104970256A CN 201510427031 A CN201510427031 A CN 201510427031A CN 104970256 A CN104970256 A CN 104970256A
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mixed solution
feed
traditional chinese
preparation
mixed
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周宗富
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Beihai City Tieshangang District Fuqun Breeding Co Ltd
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Beihai City Tieshangang District Fuqun Breeding Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The present invention discloses a preparation method of a cavia porcllus feed, which comprises the following steps: setaria viridis and bagasse are mixed, cooked and filtered to obtain a first mixed solution; bifidobacterium is inoculated into the first mixed solution to obtain a fermentation broth; artemisia argyi leaves, lotus leaves and orange peels are mixed and fried to obtain a traditional Chinese medicine mixture, the traditional Chinese medicine mixture is cooked, filtered, centrifugal separated and filtered again, and concentrated, refrigerated, and filtered to obtain a traditional Chinese medicine filtrate; the fermentation broth and the traditional Chinese medicine filtrate are mixed to obtain a second mixed solution, and the second mixed solution is boiled and cooled down; the cooled second mixed solution is concentrated and refrigerated, the obtained concentrated solution is mixed with starch to conduct granulating, and the granules are oven-dried, sterilized to obtain the cavia porcllus feed. The cavia porcllus feed prepared by the method is rich in nutrients and enables the cavia porcllus to feel a sense of satiety after being fed, bifidobacterium fermentation promotes the gastrointestinal absorption of the cavia porcllus and enables the cavia porcllus to grow rapidly, and the added traditional Chinese medicine mixture reduces the abscess probability of cavia porcllus caused by streptococcal infection, thus improving the immunity of cavia porcllus.

Description

The preparation method of black globefish feed
Technical field
The present invention relates to feed producing method, more particularly, the present invention relates to a kind of preparation method of black globefish feed.
Background technology
Black globefish, another name guinea pig, wild mountain globefish, netherlands rat, cavy, originate in South America, is a kind of globefish that build is less.Black globefish is had a sweet temper, and likes gregarious, timid, be afraid of frightened, be afraid of interference, oxyacoia, whole body black wool, ear and four limbs short and small, be not good at jumping, generally do not sting people, also do not arrest.
Ancient Chinese just has the custom of edible black globefish leopard cat meat, and it can be used as high-grade edible wild herbs meat and fish dishes to be selected into Chinese famous dish spectrum.Black globefish delicious meat is delicate, absorption easy to digest, to be among the peoplely considered as " keep fit treasure ", and fur can processing and utilization, and current research also shows, black globefish meat can promote that human normal secretes metabolism, and develop immunitypty regulates human body function.
At present, wild black globefish is progressively exhausted, and the black globefish quantity of artificial feeding cannot meet the consumption demand of people far away, therefore needs badly and finds novel black globefish feed, while guaranteeing black globefish healthy growth, accelerate the speed of growth of black globefish.
Summary of the invention
An object of the present invention is to solve at least the problems referred to above and/or defect, and the advantage will illustrated at least is below provided.
A further object of the invention is to provide a kind of preparation method of black globefish feed, in the method with fresh green bristlegrass and bagasse for primary raw material, through bifidobacterium fermentation, be added with containing tarragon, lotus leaf and orange peel Traditional Chinese drug mixture, rich in nutrition content, black globefish is made to have satiety, bifidobacterium fermentation improves the gastrointestinal absorption ability of black globefish, accelerate the speed of growth of black globefish, the interpolation of Traditional Chinese drug mixture reduces black globefish because of streptococcal infection and causes the probability of abscess, enhance the antibacterial ability of black globefish, improve the immunity of black globefish.
In order to realize, according to these objects of the present invention and other advantage, providing a kind of preparation method of black globefish feed, comprising:
Step one: green bristlegrass is mixed by quality proportioning 3: 1 ~ 4: 1 with bagasse, after pulverizing, in gained mixture, adding distil water decocts 1 ~ 2 time, has decocted rear filtration at every turn, and cooling after gained filtrate being merged, obtains the first mixed solution;
Step 2: in described first mixed solution after inoculating bifidobacterium, at 26 ~ 28 DEG C of bottom fermentation 15 ~ 20h, filter, obtain zymotic fluid, wherein, described Bifidobacterium is inoculated in described first mixed solution with the inoculum concentration that mass fraction is 1%;
Step 3: Traditional Chinese drug mixture adding distil water is decocted 2 ~ 3 times, has decocted rear filtration at every turn, centrifugation after gained mixture filtrate being merged, filters, and by the clear cream of gained coarse filtration liquid simmer down to, gets supernatant liquid filtering, drug filtrate in collection after refrigeration 10 ~ 15h;
The preparation method of described Traditional Chinese drug mixture is: tarragon, lotus leaf, orange peel are pulverized after mixing by quality proportioning 2: 2: 1, at 60 ~ 65 DEG C, after frying 5 ~ 10min, cooling is obtained;
Step 4: by described zymotic fluid and described middle drug filtrate by volume proportioning 5: 1 ~ 8: 1 mix to obtain the second mixed solution, regulate described second mixed solution pH to be 5.4 ~ 5.8, cool after described second mixed solution is boiled 5 ~ 10min;
Step 5: concentrated by cooled second mixed solution, obtain concentrate after refrigeration 12 ~ 18h, concentrate is mixed rear granulation with starch according to quality proportioning 8: 1 ~ 10: 1, sterilizing after drying, packing, preserves under room temperature.
Preferably, described in step 2, Bifidobacterium is for be inoculated in culture medium by bifidobacterium species, cultivate 2 ~ 4h at 28 ~ 32 DEG C after, described medium pH is regulated to be 4.2 ~ 4.4,1 ~ 2h is cultivated at 36 ~ 38 DEG C, regulate described medium pH to be 3.2 ~ 3.5 afterwards, obtain cultivate 1 ~ 1.5h at 32 ~ 34 DEG C after;
Wherein, described culture medium comprises the raw material of following weight fraction: 3 ~ 5 parts of coconut powders, 5 ~ 8 parts of glutinous rice flours, 1 ~ 2 part of lactose, 0.2 ~ 0.8 part of ferric citrate, 0.5 ~ 0.8 part of manganese sulfate, 0.1 ~ 0.2 part of sodium chloride, 25 ~ 35 parts of yeast extracts, 0.1 ~ 0.2 part of honey and 800 ~ 1000 parts of distilled water.
Preferably, decoct 2 times after adding distil water after pulverizing in step one, the consumption of distilled water is followed successively by 8 times amount and 6 times amount of described mixture quality, in Traditional Chinese drug mixture described in step 3, adding distil water decocts 3 times, and the consumption of distilled water is followed successively by 10 times amount of described Traditional Chinese drug mixture quality, 8 times amount and 8 times amount.
Preferably, adding distil water after decoct 2 time after pulverizing in step one, first time decocts 50min at 65 DEG C, and second time decocts 30min at 60 DEG C; In Traditional Chinese drug mixture described in step 3, adding distil water decocts 3 times, and first time decocts 60min at 85 DEG C, and second time decocts 40min at 80 DEG C, and third time decocts 30min at 78 DEG C.
Preferably, in step 3, centrifugation is operating as and controls centrifuge speed 4500 ~ 5500 revs/min, gets the supernatant after centrifugation after centrifugal 5 ~ 10min.
Preferably, filter in step 2 with step 3 in filter after centrifugation and all adopt micro porous filtration, operating pressure is 0.015MPa, and flow velocity is 2 ~ 5ml/min.
Preferably, described in step 3, the density of clear cream is 1.05g/mL.
Preferably, pH is regulated to adopt acetic acid or sodium bicarbonate in step 4.
The present invention at least comprises following beneficial effect:
1, select fresh green bristlegrass to be feed key component, wide material sources, with low cost, after adding bagasse, nutritional labeling is abundanter, makes black globefish have satiety and improves feed taste, more liking by black globefish.
2, inoculating bifidobacterium can help black globefish to suppress enteropathogenic bacteria growing, the infection of opposing cause of disease mattress, the vitamin that synthetically grown needs, improve black globefish gastrointestinal absorption ability, the beneficiating ingredient produced during the fermentation makes the nutritional labeling of feed abundanter, accelerate the speed of growth of black globefish, improve the immunity of black globefish simultaneously.
3, add containing tarragon, the Traditional Chinese drug mixture of lotus leaf and orange peel, wherein, volatile oil in tarragon, flavones, polysaccharide, trace element etc., have antibacterial, antiviral, relieving asthma, strengthen the effects such as immunologic function, alkaline components containing a lot of antimitotic effect in lotus leaf, as nuciferine, Nuciferine etc., these alkaline components have stronger bacteriostasis, black globefish antibacterial ability can be strengthened, reducing black globefish because of streptococcal infection causes the probability of abscess, in addition the carrotene be rich in orange peel and lotus leaf and vitamin C, black globefish immunity can be strengthened, make the growth that black globefish is more healthy.
4, after tarragon, lotus leaf, orange peel being pulverized at 60 ~ 65 DEG C frying 5 ~ 10min, this kind of concocting method can make the active ingredient in medicinal material more easily decoct out, improves medicinal material utilization rate.
5, the medium culture Bifidobacterium using coconut powder, glutinous rice flour etc. as key component, be conducive to Bifidobacterium survival, cultivate Bifidobacterium 2 ~ 4h at 28 ~ 32 DEG C after, 1 ~ 2h is cultivated at 36 ~ 38 DEG C, in this kind of method, temperature rises sharply, Bifidobacterium can be made better to adapt to comparatively sour environment, wherein pH is buffered acid environment 4.2 ~ 4.4, ensure that the Bifidobacterium cultivated can adapt to the environment that pH is 3.2 ~ 3.5, there is higher survival rate after entering stomach, shorten incubation time simultaneously.
6, adopt that micro porous filtration, transition speed higher than conventional filtering method precision is fast, absorption is few, to come off without medium and easy to operate.
7, green bristlegrass and bagasse are pulverized rear adding distil water and are decocted 2 times, Traditional Chinese drug mixture adding distil water decocts 3 times, wherein Traditional Chinese drug mixture decocts more times than green bristlegrass and bagasse mixture, can better make the active ingredient stripping in Traditional Chinese drug mixture, reduces Chinese medicine use amount.
8, after adopting this kind of black globefish of black globefish feed feeding, the more delicious delicacy of black globefish meat, the higher and Anticancer Element Selenium zinc of protein content and Se content increase, and make the health-care effect of black globefish meat better.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to description word to make those skilled in the art.
Embodiment 1
Step one: green bristlegrass is mixed by quality proportioning 3: 1 with bagasse, after pulverizing, in gained mixture, adding distil water decocts 1 time, 50min is decocted at 65 DEG C, the consumption of distilled water is 8 times amount of described mixture quality, filtration after decoction completes, cooling after gained filtrate being merged, obtains the first mixed solution.
Step 2: bifidobacterium species be inoculated in culture medium, after cultivating 2h, regulates described medium pH to be cultivate 1h at 4.2,36 DEG C, regulates described medium pH to be 3.2 afterwards, obtain Bifidobacterium after cultivating 1h at 32 DEG C at 28 DEG C; Wherein, described culture medium comprises the raw material of following weight fraction: 3 parts of coconut powders, 5 parts of glutinous rice flours, 1 part of lactose, 0.2 part of ferric citrate, 0.5 part of manganese sulfate, 0.1 part of sodium chloride, 25 parts of yeast extracts, 0.1 part of honey and 800 parts of distilled water.
After the inoculum concentration being 1% with mass fraction in described first mixed solution inoculates described Bifidobacterium, after 26 DEG C of bottom fermentation 15h, adopt micro porous filtration, operating pressure is 0.015MPa, and flow velocity is 2ml/min, obtains zymotic fluid.
Step 3: tarragon, lotus leaf, orange peel are pulverized after mixing by quality proportioning 2: 2: 1, the obtained Traditional Chinese drug mixture of cooling after frying 5min at 60 DEG C.
Traditional Chinese drug mixture adding distil water is decocted 2 times, first time decocts 60min at 85 DEG C, second time decocts 40min at 80 DEG C, the consumption of distilled water is followed successively by 10 times amount and 8 times amount of described Traditional Chinese drug mixture quality, filtration after each decoction completes, centrifugation after gained mixture filtrate being merged, centrifugation is operating as and controls centrifuge speed 4500 revs/min, gets the supernatant after centrifugation after centrifugal 5min.
Adopt micro porous filtration supernatant, operating pressure is 0.015MPa, and flow velocity is 2ml/min, and by the clear cream of gained coarse filtration liquid simmer down to, the density of described clear cream is 1.05g/mL, gets supernatant liquid filtering, drug filtrate in collection after refrigeration 10h.
Step 4: by described zymotic fluid and described middle drug filtrate by volume proportioning 5: 1 mix to obtain the second mixed solution, regulate described second mixed solution pH to be 5.4 with acetic acid or sodium bicarbonate, cool after described second mixed solution is boiled 5min.
Step 5: concentrated by cooled second mixed solution, obtain concentrate after refrigeration 12h, concentrate is mixed rear granulation with starch according to quality proportioning 8: 1, sterilizing after drying, packing, preserves under room temperature.
Embodiment 2
Step one: green bristlegrass is mixed by quality proportioning 4: 1 with bagasse, after pulverizing, in gained mixture, adding distil water decocts 2 times, first time decocts 50min at 65 DEG C, second time decocts 30min at 60 DEG C, the consumption of distilled water is followed successively by 8 times amount and 6 times amount of described mixture quality, filtration after each decoction completes, cooling after gained filtrate being merged, obtains the first mixed solution.
Step 2: bifidobacterium species be inoculated in culture medium, after cultivating 4h, regulates described medium pH to be cultivate 2h at 4.4,38 DEG C, regulates described medium pH to be 3.5 afterwards, obtain Bifidobacterium after cultivating 1.5h at 34 DEG C at 32 DEG C; Wherein, described culture medium comprises the raw material of following weight fraction: 5 parts of coconut powders, 8 parts of glutinous rice flours, 2 parts of lactose, 0.8 part of ferric citrate, 0.8 part of manganese sulfate, 0.2 part of sodium chloride, 35 parts of yeast extracts, 0.2 part of honey and 1000 parts of distilled water.
After the inoculum concentration being 1% with mass fraction in described first mixed solution inoculates described Bifidobacterium, after 28 DEG C of bottom fermentation 20h, adopt micro porous filtration, operating pressure is 0.015MPa, and flow velocity is 5ml/min, obtains zymotic fluid.
Step 3: tarragon, lotus leaf, orange peel are pulverized after mixing by quality proportioning 2: 2: 1, the obtained Traditional Chinese drug mixture of cooling after frying 10min at 65 DEG C.
Traditional Chinese drug mixture adding distil water is decocted 3 times, first time decocts 60min at 85 DEG C, second time decocts 40min at 80 DEG C, third time decocts 30min at 78 DEG C, the consumption of distilled water is followed successively by 10 times amount of described Traditional Chinese drug mixture quality, 8 times amount and 8 times amount, has decocted rear filtration, centrifugation after gained mixture filtrate being merged at every turn, centrifugation is operating as and controls centrifuge speed 5500 revs/min, gets the supernatant after centrifugation after centrifugal 10min.
Adopt micro porous filtration supernatant, operating pressure is 0.015MPa, and flow velocity is 5ml/min, and by the clear cream of gained coarse filtration liquid simmer down to, the density of described clear cream is 1.05g/mL, gets supernatant liquid filtering, drug filtrate in collection after refrigeration 15h.
Step 4: by described zymotic fluid and described middle drug filtrate by volume proportioning 8: 1 mix to obtain the second mixed solution, regulate described second mixed solution pH to be 5.8 with acetic acid or sodium bicarbonate, cool after described second mixed solution is boiled 10min.
Step 5: concentrated by cooled second mixed solution, obtain concentrate after refrigeration 18h, concentrate is mixed rear granulation with starch according to quality proportioning 10: 1, sterilizing after drying, packing, preserves under room temperature.
Embodiment 3
Step one: green bristlegrass is mixed by quality proportioning 3: 1 with bagasse, after pulverizing, in gained mixture, adding distil water decocts 2 times, first time decocts 50min at 65 DEG C, second time decocts 30min at 60 DEG C, the consumption of distilled water is followed successively by 8 times amount and 6 times amount of described mixture quality, filtration after each decoction completes, cooling after gained filtrate being merged, obtains the first mixed solution.
Step 2: bifidobacterium species be inoculated in culture medium, after cultivating 3h, regulates described medium pH to be cultivate 1.5h at 4.3,37 DEG C, regulates described medium pH to be 3.3 afterwards, obtain Bifidobacterium after cultivating 1.5h at 33 DEG C at 30 DEG C; Wherein, described culture medium comprises the raw material of following weight fraction: 4 parts of coconut powders, 6 parts of glutinous rice flours, 1 part of lactose, 0.5 part of ferric citrate, 0.7 part of manganese sulfate, 0.1 part of sodium chloride, 30 parts of yeast extracts, 0.1 part of honey and 900 parts of distilled water.
After the inoculum concentration being 1% with mass fraction in described first mixed solution inoculates described Bifidobacterium, after 27 DEG C of bottom fermentation 18h, adopt micro porous filtration, operating pressure is 0.015MPa, and flow velocity is 4ml/min, obtains zymotic fluid.
Step 3: tarragon, lotus leaf, orange peel are pulverized after mixing by quality proportioning 2: 2: 1, the obtained Traditional Chinese drug mixture of cooling after frying 8min at 62 DEG C.
Traditional Chinese drug mixture adding distil water is decocted 2 times, first time decocts 60min at 85 DEG C, second time decocts 40min at 80 DEG C, the consumption of distilled water is followed successively by 10 times amount and 8 times amount of described Traditional Chinese drug mixture quality, filtration after each decoction completes, centrifugation after gained mixture filtrate being merged, centrifugation is operating as and controls centrifuge speed 5000 revs/min, gets the supernatant after centrifugation after centrifugal 8min.
Adopt micro porous filtration supernatant, operating pressure is 0.015MPa, and flow velocity is 4ml/min, and by the clear cream of gained coarse filtration liquid simmer down to, the density of described clear cream is 1.05g/mL, gets supernatant liquid filtering, drug filtrate in collection after refrigeration 12h.
Step 4: by described zymotic fluid and described middle drug filtrate by volume proportioning 6: 1 mix to obtain the second mixed solution, regulate described second mixed solution pH to be 5.6 with acetic acid or sodium bicarbonate, cool after described second mixed solution is boiled 8min.
Step 5: concentrated by cooled second mixed solution, obtain concentrate after refrigeration 15h, concentrate is mixed rear granulation with starch according to quality proportioning 9: 1, sterilizing after drying, packing, preserves under room temperature.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.

Claims (8)

1. a preparation method for black globefish feed, is characterized in that, comprising:
Step one: green bristlegrass is mixed by quality proportioning 3: 1 ~ 4: 1 with bagasse, after pulverizing, in gained mixture, adding distil water decocts 1 ~ 2 time, has decocted rear filtration at every turn, and cooling after gained filtrate being merged, obtains the first mixed solution;
Step 2: in described first mixed solution after inoculating bifidobacterium, at 26 ~ 28 DEG C of bottom fermentation 15 ~ 20h, filter, obtain zymotic fluid, wherein, described Bifidobacterium is inoculated in described first mixed solution with the inoculum concentration that mass fraction is 1%;
Step 3: Traditional Chinese drug mixture adding distil water is decocted 2 ~ 3 times, has decocted rear filtration at every turn, centrifugation after gained mixture filtrate being merged, filters, and by the clear cream of gained coarse filtration liquid simmer down to, gets supernatant liquid filtering, drug filtrate in collection after refrigeration 10 ~ 15h;
The preparation method of described Traditional Chinese drug mixture is: tarragon, lotus leaf, orange peel are pulverized after mixing by quality proportioning 2: 2: 1, at 60 ~ 65 DEG C, after frying 5 ~ 10min, cooling is obtained;
Step 4: by described zymotic fluid and described middle drug filtrate by volume proportioning 5: 1 ~ 8: 1 mix to obtain the second mixed solution, regulate described second mixed solution pH to be 5.4 ~ 5.8, cool after described second mixed solution is boiled 5 ~ 10min;
Step 5: concentrated by cooled second mixed solution, obtain concentrate after refrigeration 12 ~ 18h, concentrate is mixed rear granulation with starch according to quality proportioning 8: 1 ~ 10: 1, sterilizing after drying, packing, preserves under room temperature.
2. the preparation method of black globefish feed as claimed in claim 1, it is characterized in that, described in step 2, Bifidobacterium is for be inoculated in culture medium by bifidobacterium species, cultivate 2 ~ 4h at 28 ~ 32 DEG C after, described medium pH is regulated to be 4.2 ~ 4.4, cultivate 1 ~ 2h at 36 ~ 38 DEG C, regulate described medium pH to be 3.2 ~ 3.5 afterwards, obtain cultivate 1 ~ 1.5h at 32 ~ 34 DEG C after;
Wherein, described culture medium comprises the raw material of following weight fraction: 3 ~ 5 parts of coconut powders, 5 ~ 8 parts of glutinous rice flours, 1 ~ 2 part of lactose, 0.2 ~ 0.8 part of ferric citrate, 0.5 ~ 0.8 part of manganese sulfate, 0.1 ~ 0.2 part of sodium chloride, 25 ~ 35 parts of yeast extracts, 0.1 ~ 0.2 part of honey and 800 ~ 1000 parts of distilled water.
3. the preparation method of black globefish feed as claimed in claim 2, it is characterized in that, decoct 2 times after adding distil water after pulverizing in step one, the consumption of distilled water is followed successively by 8 times amount and 6 times amount of described mixture quality, in Traditional Chinese drug mixture described in step 3, adding distil water decocts 3 times, and the consumption of distilled water is followed successively by 10 times amount of described Traditional Chinese drug mixture quality, 8 times amount and 8 times amount.
4. the preparation method of black globefish feed as claimed in claim 3, is characterized in that, adding distil water after decocts 2 time after pulverizing in step one, and first time decocts 50min at 65 DEG C, and second time decocts 30min at 60 DEG C; In Traditional Chinese drug mixture described in step 3, adding distil water decocts 3 times, and first time decocts 60min at 85 DEG C, and second time decocts 40min at 80 DEG C, and third time decocts 30min at 78 DEG C.
5. the preparation method of black globefish feed as claimed in claim 4, is characterized in that, in step 3, centrifugation is operating as and controls centrifuge speed 4500 ~ 5500 revs/min, gets the supernatant after centrifugation after centrifugal 5 ~ 10min.
6. the preparation method of black globefish feed as claimed in claim 5, is characterized in that, filter in step 2 with centrifugation in step 3 after filter and all adopt micro porous filtration, operating pressure is 0.015MPa, and flow velocity is 2 ~ 5ml/min.
7. the preparation method of black globefish feed as claimed in claim 6, it is characterized in that, described in step 3, the density of clear cream is 1.05g/mL.
8. the preparation method of black globefish feed as claimed in claim 7, is characterized in that, regulates pH to adopt acetic acid or sodium bicarbonate in step 4.
CN201510427031.XA 2015-07-20 2015-07-20 Preparation method of cavia porcllus feed Pending CN104970256A (en)

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CN106306396A (en) * 2016-08-23 2017-01-11 合肥申仁养殖有限公司 Feed for improving fur quality of black guinea pigs
CN108812531A (en) * 2018-05-29 2018-11-16 凌云县郁丰农业专业合作社联合社 A kind of disease-resistant cultural method of globefish leopard cat

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