CN107691645A - A kind of Agricus blazei composite beverage and preparation method thereof - Google Patents
A kind of Agricus blazei composite beverage and preparation method thereof Download PDFInfo
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- CN107691645A CN107691645A CN201711024996.XA CN201711024996A CN107691645A CN 107691645 A CN107691645 A CN 107691645A CN 201711024996 A CN201711024996 A CN 201711024996A CN 107691645 A CN107691645 A CN 107691645A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2240/00—Use or particular additives or ingredients
- A23C2240/15—Use of plant extracts, including purified and isolated derivatives thereof, as ingredient in dairy products
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Abstract
The present invention relates to technical field of functional drink, discloses a kind of Agricus blazei composite beverage and preparation method thereof, is made up of the raw material of following parts by weight：2~4 parts of Agricus blazei extract solution, 0.5~1 part of Pueraria Flavonid, 0.5~1 part of Pseudostellaria Polysaccharide, 0.1~0.3 part of citric acid, 0.08~0.1 part of sodium citrate, 0.3~0.6 part of maltitol, 2~4 parts of honey, 5~8 parts of sucrose, 4~6 parts of skimmed milk power, 1~1.5 part of beta cyclodextrin, 0.1~0.2 part of CMC Na, 0.1~0.2 part of sodium alginate, 0.3~1.5 part of B B-complex, 0.5~2 part of mineral matter, 0.02~0.05 part of ethylmaltol, water are to 100 parts.Physiological metabolism rule of the present invention according to adolescent growth development, the nutrition needed for adolescent growth development is gently effectively supplemented, ensures that teenager's anthropometic perfects, strengthens adolescent immunization power, improve adolescents with learning ability, improve learning efficiency.
Description
Technical field
The present invention relates to technical field of functional drink, and in particular to a kind of Agricus blazei composite beverage and preparation method thereof.
Background technology
Teenager is in puberty, and it is psychological and a series of change physiologically can all occur, its speed of growth, study
Ability, sports achievement and efficiency all have substantial connection with nutrition condition, so nutrition supply must be sufficient.It is teen-age
Nutritional need is with adult's difference, the quantity delivered of its energy and nutrient while basic physiological demand is met,
Also need meet the needs of growing, including needed of both body and intelligence.Their physiological status often changes, activity
Amount is big, if can not reach requirement in terms of diet, will directly affect or hinder their normal growth and development.It is in addition, blue or green
Teenager is faced with the examination for entering school of anxiety, is placed on without excessive energy on diet nutritional, parent often only focuses on child's achievement
Or blindly nourished various medicaments to child, can also be to teen-age body not only it cannot be guaranteed that teen-age normal growth and development
Body causes irreversible damage.Therefore, how sufficient nutrition is scientificlly and effectively supplied for teenager, reasonably met blue or green few
Year grows demand, is urgent problem to be solved.
The content of the invention
In order to solve the above problems, the invention provides a kind of Agricus blazei composite beverage and preparation method thereof, according to blue or green few
The physiological metabolism rule that year grows, the nutrition needed for adolescent growth development is gently effectively supplemented, ensures teenager's body
Lattice are physically well developed, targetedly strengthen teenager's immunity of organisms, promote the development of teenager's Brain circlulation and peripheral circulation, change
Kind adolescents with learning ability, improves adolescents with learning efficiency.
The present invention can be achieved through the following technical solutions：
A kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：2~4 parts of Agricus blazei extract solution, the root of kudzu vine are total
0.5~1 part of flavones, 0.5~1 part of Pseudostellaria Polysaccharide, 0.1~0.3 part of citric acid, 0.08~0.1 part of sodium citrate, maltitol
0.3~0.6 part, 2~4 parts of honey, 5~8 parts of sucrose, 4~6 parts of skimmed milk power, 1~1.5 part of beta-schardinger dextrin, CMC-Na 0.1~
0.2 part, 0.1~0.2 part of sodium alginate, 0.3~1.5 part of B B-complex, 0.5~2 part of mineral matter, ethylmaltol 0.02~
0.05 part, water is to 100 parts.
Preferably, a kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：Agricus blazei extract solution 2.5~
3.5 parts, 0.6~0.9 part of Pueraria Flavonid, 0.6~0.9 part of Pseudostellaria Polysaccharide, 0.15~0.25 part of citric acid, sodium citrate
0.085~0.095 part, 0.4~0.5 part of maltitol, 2.5~3.5 parts of honey, 6~7 parts of sucrose, skimmed milk power 4.5~5.5
Part, 1.2~1.3 parts of beta-schardinger dextrin, 0.12~0.18 part of CMC-Na, 0.12~0.18 part of sodium alginate, B B-complex 0.5
~1.3 parts, 1~1.5 part of mineral matter, 0.03~0.04 part of ethylmaltol, water is to 100 parts.
Preferably, a kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：3 parts of Agricus blazei extract solution, Pueraria lobota
0.75 part of root total flavone, 0.75 part of Pseudostellaria Polysaccharide, 0.2 part of citric acid, 0.09 part of sodium citrate, 0.45 part of maltitol, honeybee
It is 3 parts of honey, 6.5 parts of sucrose, 5 parts of skimmed milk power, 1.25 parts of beta-schardinger dextrin, 0.15 part of CMC-Na, 0.15 part of sodium alginate, compound
0.9 part of vitamin, 1.25 parts of mineral matter, 0.035 part of ethylmaltol, water are to 100 parts.
Preferably, the mineral matter is made up of the raw material of following parts by weight：0.05~0.1 part of calcium lactate, zinc lactate
0.01~0.05 part, 0.005~0.01 part of ironic citrate, 0.1~0.5 part of magnesium sulfate, 0.005~0.01 part of manganese sulfate, phosphoric acid
0.1~0.5 part of potassium dihydrogen, 0.05~0.2 part of sodium dihydrogen phosphate, 0.1~0.2 part of sodium chloride, 0.1~0.5 part of potassium chloride；It is described
B B-complex is made up of the raw material of following parts by weight：0.1~0.5 part of vitamin A, 0.01~0.05 part of vitamin B1,
0.01~0.05 part of vitamin B2,0.008~0.015 part of vitamin B6,0.001~0.005 part of vitamin B12, nicotinic acid
0.001~0.005 part, 0.001~0.005 part of folic acid, 0.1~0.5 part of vitamin C, 0.001~0.005 part of vitamin D,
0.1~0.2 part of vitamin E.
Preferably, the mineral matter is made up of the raw material of following parts by weight：0.06~0.09 part of calcium lactate, zinc lactate
0.02~0.04 part, 0.0065~0.0085 part of ironic citrate, 0.2~0.4 part of magnesium sulfate, manganese sulfate 0.0055~0.0095
Part, 0.2~0.4 part of potassium dihydrogen phosphate, 0.1~0.15 part of sodium dihydrogen phosphate, 0.12~0.18 part of sodium chloride, potassium chloride 0.2~
0.4 part；The B B-complex is made up of the raw material of following parts by weight：0.2~0.4 part of vitamin A, vitamin B1 0.02
~0.04 part, 0.02~0.04 part of vitamin B2,0.01~0.013 part of vitamin B6, vitamin B12 0.002~0.004
Part, 0.002~0.004 part of nicotinic acid, 0.002~0.004 part of folic acid, 0.2~0.4 part of vitamin C, vitamin D 0.002~
0.004 part, 0.13~0.17 part of vitamin E.
Preferably, the mineral matter is made up of the raw material of following parts by weight：0.075 part of calcium lactate, 0.03 part of zinc lactate,
0.0075 part of ironic citrate, 0.3 part of magnesium sulfate, 0.0075 part of manganese sulfate, 0.3 part of potassium dihydrogen phosphate, sodium dihydrogen phosphate 0.125
Part, 0.15 part of sodium chloride, 0.3 part of potassium chloride；The B B-complex is made up of the raw material of following parts by weight：Vitamin A
0.3 part, 0.03 part of vitamin B1,0.03 part of vitamin B2,0.0115 part of vitamin B6,0.003 part of vitamin B12, cigarette
0.003 part of acid, 0.003 part of folic acid, 0.3 part of vitamin C, 0.003 part of vitamin D, 0.15 part of vitamin E.
Preferably, the preparation method of the Agricus blazei extract solution is：By mycelium inoculation in by potato 200g, glucose
The middle part for the slant medium that 20g, peptone 20g, agar 20g, water 1000mL are formed, 10d is cultivated at 25 DEG C, will be by corn
Powder 2%, yeast extract 0.3%, sucrose 1%, glucose 1%, potassium dihydrogen phosphate 1%, magnesium sulfate 1%, 0.01% group of vitamin B1
Into the liquid fermentation medium that pH is 5~6 is respectively charged into one-level, second-level shake flask, and the strain after culture activation of learning from else's experience is placed in one
In level shaking flask, at 25 DEG C, 7~8d is cultivated under conditions of 160r/min, takes 10% one-level shaking flask strain to be placed in second-level shake flask,
26 DEG C, 5d is cultivated under conditions of 150r/min, then zymotic fluid is put into bruisher together with mycelia and is broken into slurries, will
Slurries extract 2 times, each 30min in 60 DEG C of water-baths, filtering, merging filtrate, centrifuge 15min through 4000r/min, take supernatant
Liquid, as Agricus blazei extract solution.
Preferably, the preparation method of the Pueraria Flavonid is：Take the root of kudzu vine to be cleaned with clear water, cut into pieces, be placed in 80 DEG C
Dried in baking oven, crushed 80 mesh sieves, take root of kudzu vine powder, be 1 by solid-liquid ratio:10 add 70% ethanol, at 50 DEG C, surpass under 300W
Sound extracts 3 times, each 30min, merges extract solution, ethanol is recovered under reduced pressure, rotary evaporation is concentrated into original volume 1/10, takes concentrate
It is 1.5g/mL to add water regulation sample concentration, with 1mL/min flow velocity by HPD-750 macroporous resin columns, is washed to distilled water
After eluent is colourless, with 5BV 70% ethanol elution, ethanol eluate is collected, ethanol is recovered under reduced pressure, in 65 DEG C of vacuum drying chambers
Middle drying, obtains Pueraria Flavonid.
Preferably, the preparation method of the Pseudostellaria Polysaccharide is：Radix pseudostellariae medicinal material is taken, dry, pulverize in 60 DEG C of drying boxes
40 mesh sieves are crossed, weigh Heterophylly Falsesatarwort Root 50g, after 350mL distilled water immersions 24h, ultrasonic extraction 2 times, each 30min, are merged
Extract solution, filtering, take filtrate to be concentrated into the 1/5 of original volume, 4 times of volume absolute ethyl alcohols are added, after standing overnight, through 3000r/
Min centrifuges 30min, takes precipitation to be placed in heat drying in 65 DEG C of water-baths, obtains Pseudostellaria Polysaccharide crude product, takes Pseudostellaria Polysaccharide thick
After product add isometric water to dissolve, and by trichloroacetic acid:N-butanol=5:The mixed solution of 1 composition presses 1:1 ratio mixing, stands
4h, 10min is centrifuged through 4000r/min, take supernatant distilled water dialysis 24h, then added 3 times of volume absolute ethyl alcohols and stood
At night, 10min is centrifuged through 4000r/min, takes precipitation to be washed with absolute ethyl alcohol, volatilizes ethanol, crown prince is obtained in 65 DEG C of vacuum drying
Gracilis polysaccharide.
The present invention also provides a kind of preparation method of Agricus blazei composite beverage, comprises the following steps：
(5) it is 1 by solid-liquid ratio by skimmed milk power:5 add water, stir to being completely dissolved, and add Agricus blazei by weight and carry
Take liquid, Pueraria Flavonid, Pseudostellaria Polysaccharide to stir, then by weight add sucrose, maltitol, honey, citric acid and
Sodium citrate, it is stirred into mixed liquor；
(6) CMC-Na, sodium alginate, beta-schardinger dextrin is taken to add 20~40mL water and be heated to being completely dissolved, addition mineral matter,
After B B-complex and ethylmaltol stir, it is added in step (1) mixed liquor and is stirred into beverage；
(7) deployed beverage is placed in homogeneous 2 times under 15~20MPa, then deaerated in the case where vacuum is 0.05MPa
10min；
(8) by the beverage after degassing at 115 DEG C high-temperature short-time sterilization 5s, then vacuum filling, is cooled to room temperature, obtains
Agricus blazei composite beverage.
Each main composition mechanism of action of the present invention is as follows：
Agricus blazei contains abundant protein, carbohydrate and vitamin, and nutritive value is higher, to nospecific immunity and specifically
Property immune have significant humidification.
The root of kudzu vine can leniently improve Brain circlulation and peripheral circulation, improve the arrested state of retinal vessel tip unit, also
Immunization, improving studing ability can be strengthened, its kudzu root flavone contained can promote the propagation of adolescent bone cell, increase
Add bone density and bone mineral content, promote the formation of bone.
Radix pseudostellariae contains various active composition, can increase peripheral white blood cell amount, stimulate circulation, and strengthens immune make
With protection cell is complete, has certain resisting stress and antifatigue effect, can also improve low temperature tolerance ability.
The present invention possesses following beneficial effect：
1st, the present invention uses fermented and cultured-water-bath restricted-access media Agricus blazei extract solution, the Agricus blazei extract solution tool of gained
There is significant antitumor activity, add it in composite beverage, obtained composite beverage is contained abundant protein, sugar
The nutriment such as class and vitamin, can fully meet adolescent growth development needed for nutrition, improve adolescent immunization power, it is perfect
Adolescent immunization system, keeps fit.
2nd, for the present invention using ultrasonic wave added method extraction Pueraria Flavonid, the Pueraria Flavonid of gained, which has, promotes nervous system
The effect of activity, is added it in composite beverage, and the beverage of gained can gently be effectively facilitated teenager's Brain circlulation and outer
Zhou Xunhuan development, teen-age thought active degree is lifted, is improved learning efficiency.
3rd, the present invention especially selects Pseudostellaria Polysaccharide, and the B B-complex, mineral matter compounding with rational proportion make compound
Beverage, obtained composite beverage can teenager supplement growth needed for nutrition, promote teenager's blood circulation, improve teenager it is thin
Cytoactive, increase teenager's resisting stress and anti-fatigue ability.
4th, Agricus blazei extract solution and Pueraria Flavonid, Pseudostellaria Polysaccharide compounding are made composite beverage by the present invention, obtained
Composite beverage can targetedly improve adolescent bone and the development of cartilage cell according to teenager's bone growth-development law
State, promote adolescent growth, maintain teenager's health.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：2 parts of Agricus blazei extract solution, the root of kudzu vine are always yellow
0.5 part of ketone, 0.5 part of Pseudostellaria Polysaccharide, 0.1 part of citric acid, 0.08 part of sodium citrate, 0.3 part of maltitol, 2 parts of honey, sucrose
5 parts, 4 parts of skimmed milk power, 1 part of beta-schardinger dextrin, 0.1 part of CMC-Na, 0.1 part of sodium alginate, 0.3 part of B B-complex, mineral matter
0.5 part, 0.02 part of ethylmaltol, water is to 100 parts.
The mineral matter is made up of the raw material of following parts by weight：0.05 part of calcium lactate, 0.01 part of zinc lactate, ironic citrate
0.005 part, 0.1 part of magnesium sulfate, 0.005 part of manganese sulfate, 0.1 part of potassium dihydrogen phosphate, 0.05 part of sodium dihydrogen phosphate, sodium chloride 0.1
Part, 0.1 part of potassium chloride；The B B-complex is made up of the raw material of following parts by weight：0.1 part of vitamin A, vitamin B1
0.01 part, 0.01 part of vitamin B2,0.008 part of vitamin B6,0.001 part of vitamin B12,0.001 part of nicotinic acid, folic acid
0.001 part, 0.1 part of vitamin C, 0.001 part of vitamin D, 0.1 part of vitamin E.
The preparation method of the Agricus blazei extract solution is：By mycelium inoculation in by potato 200g, glucose 20g, albumen
The middle part for the slant medium that peptone 20g, agar 20g, water 1000mL are formed, 10d is cultivated at 25 DEG C, will be by corn flour 2%, ferment
Female cream 0.3%, sucrose 1%, glucose 1%, potassium dihydrogen phosphate 1%, magnesium sulfate 1%, vitamin B1 0.01% form, pH 5
~6 liquid fermentation medium is respectively charged into one-level, second-level shake flask, and the strain after culture activation of learning from else's experience is placed in one-level shaking flask
In, at 25 DEG C, 7~8d is cultivated under conditions of 160r/min, takes 10% one-level shaking flask strain to be placed in second-level shake flask, at 26 DEG C,
5d is cultivated under conditions of 150r/min, then zymotic fluid is put into bruisher together with mycelia and is broken into slurries, by slurries
Extract 2 times, each 30min, filter, merging filtrate in 60 DEG C of water-baths, centrifuge 15min through 4000r/min, take supernatant,
As Agricus blazei extract solution.
The preparation method of the Pueraria Flavonid is：Take the root of kudzu vine to be cleaned with clear water, cut into pieces, be placed in 80 DEG C of baking ovens and dry
It is dry, 80 mesh sieves are crushed, take root of kudzu vine powder, are 1 by solid-liquid ratio:10 add 70% ethanol, at 50 DEG C, ultrasonic extraction 3 under 300W
It is secondary, each 30min, merge extract solution, ethanol is recovered under reduced pressure, rotary evaporation is concentrated into original volume 1/10, takes concentrate to add water to adjust
Section sample concentration is 1.5g/mL, and with 1mL/min flow velocity by HPD-750 macroporous resin columns, eluent is washed to distilled water
After colourless, with 5BV 70% ethanol elution, ethanol eluate collected, ethanol is recovered under reduced pressure, done in 65 DEG C of vacuum drying chambers
It is dry, obtain Pueraria Flavonid.
The preparation method of the Pseudostellaria Polysaccharide is：Radix pseudostellariae medicinal material is taken, 40 mesh were dry, pulverize in 60 DEG C of drying boxes
Sieve, weighs Heterophylly Falsesatarwort Root 50g, after 350mL distilled water immersions 24h, ultrasonic extraction 2 times, each 30min, merges extract solution,
Filtering, take filtrate to be concentrated into the 1/5 of original volume, add 4 times of volume absolute ethyl alcohols, after standing overnight, centrifuged through 3000r/min
30min, take precipitation to be placed in heat drying in 65 DEG C of water-baths, obtain Pseudostellaria Polysaccharide crude product, take Pseudostellaria Polysaccharide crude product to add
After volume of water dissolving, and by trichloroacetic acid:N-butanol=5:The mixed solution of 1 composition presses 1:1 ratio mixing, stands 4h, warp
4000r/min centrifuges 10min, takes supernatant distilled water dialysis 24h, then adds 3 times of volume absolute ethyl alcohols and stands overnight, passes through
4000r/min centrifuges 10min, takes precipitation to be washed with absolute ethyl alcohol, volatilizes ethanol, and it is more to obtain radix pseudostellariae in 65 DEG C of vacuum drying
Sugar.
A kind of preparation method of above-mentioned Agricus blazei composite beverage, comprises the following steps：
(1) it is 1 by solid-liquid ratio by skimmed milk power:5 add water, stir to being completely dissolved, and add Agricus blazei by weight and carry
Take liquid, Pueraria Flavonid, Pseudostellaria Polysaccharide to stir, then by weight add sucrose, maltitol, honey, citric acid and
Sodium citrate, it is stirred into mixed liquor；
(2) CMC-Na, sodium alginate, beta-schardinger dextrin is taken to add 20~40mL water and be heated to being completely dissolved, addition mineral matter,
After B B-complex and ethylmaltol stir, it is added in step (1) mixed liquor and is stirred into beverage；
(3) deployed beverage is placed in homogeneous 2 times under 15~20MPa, then deaerated in the case where vacuum is 0.05MPa
10min；
(4) by the beverage after degassing at 115 DEG C high-temperature short-time sterilization 5s, then vacuum filling, is cooled to room temperature, obtains
Agricus blazei composite beverage.
Embodiment 2
A kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：2.5 parts of Agricus blazei extract solution, the root of kudzu vine are total
0.6 part of flavones, 0.6 part of Pseudostellaria Polysaccharide, 0.15 part of citric acid, 0.085 part of sodium citrate, 0.4 part of maltitol, honey 2.5
Part, 6 parts of sucrose, 4.5 parts of skimmed milk power, 1.2 parts of beta-schardinger dextrin, 0.12 part of CMC-Na, 0.12 part of sodium alginate, compound dimension life
0.5 part of element, 1 part of mineral matter, 0.03 part of ethylmaltol, water are to 100 parts.
The mineral matter is made up of the raw material of following parts by weight：0.06 part of calcium lactate, 0.02 part of zinc lactate, ironic citrate
0.0065 part, 0.2 part of magnesium sulfate, 0.0055 part of manganese sulfate, 0.2 part of potassium dihydrogen phosphate, 0.1 part of sodium dihydrogen phosphate, sodium chloride
0.12 part, 0.2 part of potassium chloride；The B B-complex is made up of the raw material of following parts by weight：0.2 part of vitamin A, dimension life
Plain 0.02 part of B1,0.02 part of vitamin B2,0.01 part of vitamin B6,0.002 part of vitamin B12,0.002 part of nicotinic acid, leaf
0.002 part of acid, 0.2 part of vitamin C, 0.002 part of vitamin D, 0.13 part of vitamin E.
Embodiment 3
A kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：3 parts of Agricus blazei extract solution, the root of kudzu vine are always yellow
0.75 part of ketone, 0.75 part of Pseudostellaria Polysaccharide, 0.2 part of citric acid, 0.09 part of sodium citrate, 0.45 part of maltitol, 3 parts of honey,
6.5 parts of sucrose, 5 parts of skimmed milk power, 1.25 parts of beta-schardinger dextrin, 0.15 part of CMC-Na, 0.15 part of sodium alginate, B B-complex
0.9 part, 1.25 parts of mineral matter, 0.035 part of ethylmaltol, water is to 100 parts.
The mineral matter is made up of the raw material of following parts by weight：0.075 part of calcium lactate, 0.03 part of zinc lactate, citric acid
0.0075 part of iron, 0.3 part of magnesium sulfate, 0.0075 part of manganese sulfate, 0.3 part of potassium dihydrogen phosphate, 0.125 part of sodium dihydrogen phosphate, chlorination
0.15 part of sodium, 0.3 part of potassium chloride；The B B-complex is made up of the raw material of following parts by weight：0.3 part of vitamin A, dimension
Raw plain 0.03 part of B1,0.03 part of vitamin B2,0.0115 part of vitamin B6,0.003 part of vitamin B12, nicotinic acid 0.003
Part, 0.003 part of folic acid, 0.3 part of vitamin C, 0.003 part of vitamin D, 0.15 part of vitamin E.
Embodiment 4
A kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：3.5 parts of Agricus blazei extract solution, the root of kudzu vine are total
0.9 part of flavones, 0.9 part of Pseudostellaria Polysaccharide, 0.25 part of citric acid, 0.095 part of sodium citrate, 0.5 part of maltitol, honey 3.5
Part, 7 parts of sucrose, 5.5 parts of skimmed milk power, 1.3 parts of beta-schardinger dextrin, 0.18 part of CMC-Na, 0.18 part of sodium alginate, compound dimension life
1.3 parts of element, 1.5 parts of mineral matter, 0.04 part of ethylmaltol, water are to 100 parts.
The mineral matter is made up of the raw material of following parts by weight：0.09 part of calcium lactate, 0.04 part of zinc lactate, ironic citrate
0.0085 part, 0.4 part of magnesium sulfate, 0.0095 part of manganese sulfate, 0.4 part of potassium dihydrogen phosphate, 0.15 part of sodium dihydrogen phosphate, sodium chloride
0.18 part, 0.4 part of potassium chloride；The B B-complex is made up of the raw material of following parts by weight：0.4 part of vitamin A, dimension life
Plain 0.04 part of B1,0.04 part of vitamin B2,0.013 part of vitamin B6,0.004 part of vitamin B12,0.004 part of nicotinic acid, leaf
0.004 part of acid, 0.4 part of vitamin C, 0.004 part of vitamin D, 0.17 part of vitamin E.
Embodiment 5
A kind of Agricus blazei composite beverage, is made up of the raw material of following parts by weight：4 parts of Agricus blazei extract solution, the root of kudzu vine are always yellow
1 part of ketone, 1 part of Pseudostellaria Polysaccharide, 0.3 part of citric acid, 0.1 part of sodium citrate, 0.6 part of maltitol, 4 parts of honey, 8 parts of sucrose,
6 parts of skimmed milk power, 1.5 parts of beta-schardinger dextrin, 0.2 part of CMC-Na, 0.2 part of sodium alginate, 1.5 parts of B B-complex, mineral matter 2
Part, 0.05 part of ethylmaltol, water are to 100 parts.
The mineral matter is made up of the raw material of following parts by weight：0.1 part of calcium lactate, 0.05 part of zinc lactate, ironic citrate
0.01 part, 0.5 part of magnesium sulfate, 0.01 part of manganese sulfate, 0.5 part of potassium dihydrogen phosphate, 0.2 part of sodium dihydrogen phosphate, 0.2 part of sodium chloride,
0.5 part of potassium chloride；The B B-complex is made up of the raw material of following parts by weight：0.5 part of vitamin A, vitamin B1
0.05 part, 0.05 part of vitamin B2,0.015 part of vitamin B6,0.005 part of vitamin B12,0.005 part of nicotinic acid, folic acid
0.005 part, 0.5 part of vitamin C, 0.005 part of vitamin D, 0.2 part of vitamin E.
Comparative example 1
Difference with embodiment 1 is, without addition Agricus blazei extract solution in composition of raw materials, is specially：Pueraria Flavonid
0.5 part, 0.5 part of Pseudostellaria Polysaccharide, 0.1 part of citric acid, 0.08 part of sodium citrate, 0.3 part of maltitol, 2 parts of honey, sucrose 5
Part, 4 parts of skimmed milk power, 1 part of beta-schardinger dextrin, 0.1 part of CMC-Na, 0.1 part of sodium alginate, 0.3 part of B B-complex, mineral matter
0.5 part, 0.02 part of ethylmaltol, water is to 100 parts.
Comparative example 2
Difference with embodiment 1 is, without addition Pueraria Flavonid in composition of raw materials, is specially：Agricus blazei extract solution 2
Part, 0.5 part of Pseudostellaria Polysaccharide, 0.1 part of citric acid, 0.08 part of sodium citrate, 0.3 part of maltitol, 2 parts of honey, 5 parts of sucrose,
4 parts of skimmed milk power, 1 part of beta-schardinger dextrin, 0.1 part of CMC-Na, 0.1 part of sodium alginate, 0.3 part of B B-complex, mineral matter 0.5
Part, 0.02 part of ethylmaltol, water are to 100 parts.
Comparative example 3
Difference with embodiment 1 is, without addition Pseudostellaria Polysaccharide in composition of raw materials, is specially：Agricus blazei extract solution 2
Part, 0.5 part of Pueraria Flavonid, 0.1 part of citric acid, 0.08 part of sodium citrate, 0.3 part of maltitol, 2 parts of honey, 5 parts of sucrose,
4 parts of skimmed milk power, 1 part of beta-schardinger dextrin, 0.1 part of CMC-Na, 0.1 part of sodium alginate, 0.3 part of B B-complex, mineral matter 0.5
Part, 0.02 part of ethylmaltol, water are to 100 parts.
Efficacy validation
Test example 1
The composite beverage for taking 1-5 of the embodiment of the present invention and comparative example 1-3 to prepare carries out nutrient composition content measure.Experiment knot
Fruit is as shown in table 1 below：
Nutrient composition content in beverage prepared by the present invention of table 1
| Group | Protein (g/100g) | Mineral matter (mg/100g) | Amino acid (mg/100g) |
| Embodiment 1 | 9.87 | 257.5 | 419.8 |
| Embodiment 2 | 10.59 | 263.6 | 421.3 |
| Embodiment 3 | 11.26 | 278.9 | 437.4 |
| Embodiment 4 | 10.64 | 259.4 | 418.3 |
| Embodiment 5 | 9.26 | 253.8 | 412.6 |
| Comparative example 1 | 6.54 | 197.6 | 363.5 |
| Comparative example 2 | 7.25 | 185.1 | 354.2 |
| Comparative example 3 | 7.83 | 177.8 | 371.4 |
As seen from the above table, by the contrast of embodiment 1 and comparative example 1, a Ji is added in composite beverage prepared by the present invention
Matsutake extract solution, the content of protein, mineral matter and amino acid in beverage can be respectively increased to 33.7%, 23.3% and
13.4%；By the contrast of embodiment 1 and comparative example 2, Pueraria Flavonid is added in composite beverage prepared by the present invention,
The content of protein, mineral matter and amino acid in beverage can be respectively increased 26.5%, 28.1% and 15.6%；Pass through embodiment
1 is understood with the contrast of comparative example 3, and Pseudostellaria Polysaccharide is added in composite beverage prepared by the present invention, can by protein in beverage,
The content of mineral matter and amino acid is respectively increased 20.7%, 30.9% and 11.5%.
In summary, Agricus blazei extract solution, Pueraria Flavonid and radix pseudostellariae are added in composite beverage prepared by the present invention
Polysaccharide, the nutrient composition contents such as protein in beverage, mineral matter and amino acid are remarkably improved, supplement adolescent growth hair in time
Educate required nutriment.
Test example 2
30 inferior health teenagers of selection are observed, age 13-15 year, the suitable Asia of simultaneous selection physical condition
As a control group, day-to-day test person eats composite beverage prepared by the embodiment of the present invention 1 and common to healthy adolescent 30 respectively
Sports drink 500ml.Experimental result such as table 2 below：
Table 2
As seen from the above table, the composite beverage of index of the present invention is eaten after 3 months, 76% person's of participating in the experiment sufficient sleeping, the youth
Acne, freckle are reduced, memory increase, and the disease frequency such as flu is reduced；After 6 months, 84% person's of participating in the experiment sufficient sleeping is blue or green
Spring acne, freckle are reduced, memory increase, and the disease frequency such as flu is reduced；After 9 months, 92% person's of participating in the experiment sufficient sleeping,
Whelk, freckle are almost disappeared, memory increase, and the disease frequency such as flu is reduced, and various subhealth symptoms almost disappear.
And the edible teenager as a control group of popular sports drink is used, S＆S changes without obvious.
In summary, the composite beverage that prepared by the present invention, teen-age whelk, freckle can be eliminated, promotes teenager god
Development through system, improving studing ability, while immunity of organisms can be strengthened, the generation of the diseases such as flu is reduced, to green grass or young crops
Juvenile healthy growth has certain help.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.
Claims (10)
1. a kind of Agricus blazei composite beverage, it is characterised in that be made up of the raw material of following parts by weight：Agricus blazei extract solution 2~4
Part, 0.5~1 part of Pueraria Flavonid, 0.5~1 part of Pseudostellaria Polysaccharide, 0.1~0.3 part of citric acid, sodium citrate 0.08~0.1
Part, 0.3~0.6 part of maltitol, 2~4 parts of honey, 5~8 parts of sucrose, 4~6 parts of skimmed milk power, 1~1.5 part of beta-schardinger dextrin,
CMC-Na0.1~0.2 part, 0.1~0.2 part of sodium alginate, 0.3~1.5 part of B B-complex, 0.5~2 part of mineral matter, ethyl
0.02~0.05 part of maltol, water are to 100 parts.
2. a kind of Agricus blazei composite beverage according to claim 1, it is characterised in that by the raw material group of following parts by weight
Into：2.5~3.5 parts of Agricus blazei extract solution, 0.6~0.9 part of Pueraria Flavonid, 0.6~0.9 part of Pseudostellaria Polysaccharide, citric acid
0.15~0.25 part, 0.085~0.095 part of sodium citrate, 0.4~0.5 part of maltitol, 2.5~3.5 parts of honey, sucrose 6~
7 parts, 4.5~5.5 parts of skimmed milk power, 1.2~1.3 parts of beta-schardinger dextrin, 0.12~0.18 part of CMC-Na, sodium alginate 0.12~
0.18 part, 0.5~1.3 part of B B-complex, 1~1.5 part of mineral matter, 0.03~0.04 part of ethylmaltol, water is to 100 parts.
3. a kind of Agricus blazei composite beverage according to claim 2, it is characterised in that by the raw material group of following parts by weight
Into：3 parts of Agricus blazei extract solution, 0.75 part of Pueraria Flavonid, 0.75 part of Pseudostellaria Polysaccharide, 0.2 part of citric acid, sodium citrate 0.09
Part, 0.45 part of maltitol, 3 parts of honey, 6.5 parts of sucrose, 5 parts of skimmed milk power, 1.25 parts of beta-schardinger dextrin, CMC-Na0.15 parts,
0.15 part of sodium alginate, 0.9 part of B B-complex, 1.25 parts of mineral matter, 0.035 part of ethylmaltol, water are to 100 parts.
A kind of 4. Agricus blazei composite beverage according to any one of claims 1 to 3, it is characterised in that the mineral matter by
The raw material composition of following parts by weight：0.05~0.1 part of calcium lactate, 0.01~0.05 part of zinc lactate, ironic citrate 0.005~
0.01 part, 0.1~0.5 part of magnesium sulfate, 0.005~0.01 part of manganese sulfate, 0.1~0.5 part of potassium dihydrogen phosphate, sodium dihydrogen phosphate
0.05~0.2 part, 0.1~0.2 part of sodium chloride, 0.1~0.5 part of potassium chloride；The B B-complex by following parts by weight original
Material forms：0.1~0.5 part of vitamin A, 0.01~0.05 part of vitamin B1,0.01~0.05 part of vitamin B2, dimension life
Plain 0.008~0.015 part of B6,0.001~0.005 part of vitamin B12,0.001~0.005 part of nicotinic acid, folic acid 0.001~
0.005 part, 0.1~0.5 part of vitamin C, 0.001~0.005 part of vitamin D, 0.1~0.2 part of vitamin E.
5. a kind of Agricus blazei composite beverage according to claim 4, it is characterised in that the mineral matter is by following parts by weight
Raw material composition：0.06~0.09 part of calcium lactate, 0.02~0.04 part of zinc lactate, 0.0065~0.0085 part of ironic citrate,
0.2~0.4 part of magnesium sulfate, 0.0055~0.0095 part of manganese sulfate, 0.2~0.4 part of potassium dihydrogen phosphate, sodium dihydrogen phosphate 0.1~
0.15 part, 0.12~0.18 part of sodium chloride, 0.2~0.4 part of potassium chloride；The B B-complex by following parts by weight raw material
Composition：0.2~0.4 part of vitamin A, 0.02~0.04 part of vitamin B1,0.02~0.04 part of vitamin B2, vitamin B6
0.01~0.013 part, 0.002~0.004 part of vitamin B12,0.002~0.004 part of nicotinic acid, 0.002~0.004 part of folic acid,
0.2~0.4 part of vitamin C, 0.002~0.004 part of vitamin D, 0.13~0.17 part of vitamin E.
6. a kind of Agricus blazei composite beverage according to claim 5, it is characterised in that the mineral matter is by following parts by weight
Raw material composition：0.075 part of calcium lactate, 0.03 part of zinc lactate, 0.0075 part of ironic citrate, 0.3 part of magnesium sulfate, manganese sulfate
0.0075 part, 0.3 part of potassium dihydrogen phosphate, 0.125 part of sodium dihydrogen phosphate, 0.15 part of sodium chloride, 0.3 part of potassium chloride；It is described compound
Vitamin is made up of the raw material of following parts by weight：0.3 part of vitamin A, 0.03 part of vitamin B1,0.03 part of vitamin B2,
0.0115 part of vitamin B6,0.003 part of vitamin B12,0.003 part of nicotinic acid, 0.003 part of folic acid, 0.3 part of vitamin C, dimension
Raw plain 0.003 part of D, 0.15 part of vitamin E.
7. a kind of Agricus blazei composite beverage according to any one of claims 1 to 3, it is characterised in that the Agricus blazei carries
The preparation method for taking liquid is：By mycelium inoculation in by potato 200g, glucose 20g, peptone 20g, agar 20g, water
The middle part of the slant medium of 1000mL compositions, 10d is cultivated at 25 DEG C, will be by corn flour 2%, yeast extract 0.3%, sucrose
1%th, glucose 1%, potassium dihydrogen phosphate 1%, magnesium sulfate 1%, vitamin B1 0.01% form, and pH is 5~6 liquid fermentation
Culture medium is respectively charged into one-level, second-level shake flask, and the strain after culture activation of learning from else's experience is placed in one-level shaking flask, at 25 DEG C, 160r/
7~8d is cultivated under conditions of min, takes 10% one-level shaking flask strain to be placed in second-level shake flask, at 26 DEG C, under conditions of 150r/min
5d is cultivated, then zymotic fluid is put into bruisher together with mycelia and is broken into slurries, slurries are soaked in 60 DEG C of water-baths
Carry 2 times, each 30min, filter, merging filtrate, centrifuge 15min through 4000r/min, take supernatant, as Agricus blazei extract solution.
8. a kind of Agricus blazei composite beverage according to any one of claims 1 to 3, it is characterised in that the root of kudzu vine is always yellow
The preparation method of ketone is：Take the root of kudzu vine to be cleaned with clear water, cut into pieces, be placed in 80 DEG C of baking ovens and dry, crushed 80 mesh sieves, take Pueraria lobota
Root powder, it is 1 by solid-liquid ratio:10 add 70% ethanol, at 50 DEG C, ultrasonic extraction 3 times under 300W, each 30min, merge extraction
Liquid, ethanol being recovered under reduced pressure, rotary evaporation is concentrated into original volume 1/10, takes concentrate to add water regulation sample concentration to be 1.5g/mL, with
1mL/min flow velocity by HPD-750 macroporous resin columns, with distilled water be washed to eluent it is colourless after, with 5BV 70% ethanol
Elution, ethanol eluate is collected, ethanol is recovered under reduced pressure, dried in 65 DEG C of vacuum drying chambers, obtain Pueraria Flavonid.
9. a kind of Agricus blazei composite beverage according to any one of claims 1 to 3, it is characterised in that the radix pseudostellariae is more
Sugar preparation method be：Radix pseudostellariae medicinal material is taken, 40 mesh sieves was dry, pulverize in 60 DEG C of drying boxes, and weighed Heterophylly Falsesatarwort Root 50g,
After 350mL distilled water immersions 24h, ultrasonic extraction 2 times, each 30min, merge extract solution, filtering, take filtrate to be concentrated into substance
Long-pending 1/5,4 times of volume absolute ethyl alcohols are added, after standing overnight, centrifuge 30min through 3000r/min, take precipitation to be placed in 65 DEG C of water
Heat drying in bath, Pseudostellaria Polysaccharide crude product is obtained, after taking Pseudostellaria Polysaccharide crude product to add isometric water to dissolve, and by three chloroethenes
Acid:N-butanol=5:The mixed solution of 1 composition presses 1:1 ratio mixing, stands 4h, centrifuges 10min through 4000r/min, takes
Clear liquid distilled water dialysis 24h, then adds 3 times of volume absolute ethyl alcohols and stands overnight, centrifuge 10min through 4000r/min, it is heavy to take
Shallow lake is washed with absolute ethyl alcohol, volatilizes ethanol, and Pseudostellaria Polysaccharide is obtained in 65 DEG C of vacuum drying.
A kind of 10. method of the Agricus blazei composite beverage as described in any one of claim 1~9, it is characterised in that including following step
Suddenly：
(1) it is 1 by solid-liquid ratio by skimmed milk power:5 add water, stir to being completely dissolved, add by weight Agricus blazei extract solution,
Pueraria Flavonid, Pseudostellaria Polysaccharide stir, then add sucrose, maltitol, honey, citric acid and citric acid by weight
Sodium, it is stirred into mixed liquor；
(2) take CMC-Na, sodium alginate, beta-schardinger dextrin to add 20~40mL water and be heated to being completely dissolved, add mineral matter, compound
After vitamin and ethylmaltol stir, it is added in step (1) mixed liquor and is stirred into beverage；
(3) deployed beverage is placed in homogeneous 2 times under 15~20MPa, then deaerate 10min in the case where vacuum is 0.05MPa；
(4) by the beverage after degassing at 115 DEG C high-temperature short-time sterilization 5s, then vacuum filling, is cooled to room temperature, obtains Ji Song
Fine and soft composite beverage.
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