KR100910504B1 - The cultivation method of the high hardness and sugar content strawberry having the high content of vitamin and calcium - Google Patents

Abstract

A method for growing strawberries is provided to secure the contents of vitamin and calcium and to improve the hardness and sweetness by using a fermented seaweed liquid and a microorganism culture liquid. A method for growing strawberries comprises the following steps of: foliar-applying a first fermented seaweed liquid every 15-20 days from the blooming of flower cluster; irrigating a first microorganism culture liquid every 7-10 days after transplantation; foliar-applying a second microorganism culture liquid every 15-20 days from the blooming of flower cluster; and irrigating an ionized calcium agent once or twice 15 days before harvest. The first fermented seaweed liquid contains 15wt% of seaweed powder with Bacillus licheniformis ATCC 102 culture fluid. The first microorganism culture liquid is obtained by inoculating 5wt% of Bacillus subtilis ATCC 4529 in a culture medium containing 2wt% of blood meal, 10wt% of fish meal, 1wt% of glucose, 5wt% of molasses, 0.5wt% of K2HPO4, 1wt% of CSR and water and cultivating it. The second microorganism culture liquid is obtained by inoculating 5wt% of Bacillus atrophaeus ATCC 7972 in a culture medium containing 0.03wt% of beef extract, 0.05wt% of peptone, 10wt% of soybean scum, 1wt% of glucose, 5wt% of molasses, 0.5wt% of K2HPO4, 1wt% of CSR and water and cultivating it.

Description

The cultivation method of the high hardness and sugar content strawberry having the high content of vitamin and calcium}

The present invention relates to a method of cultivating strawberries with high content of vitamins and calcium, and excellent hardness and sugar, and more specifically, Bacillus licheniformis ATCC 102 ( Bacillus licheniformis ATCC 102) culture medium in a 15% by weight aqueous solution of seaweed powder. Addition of fermented algae fermentation broth 1 to the foliar spray at intervals of 15 to 20 days from the flowering of each flower, fermented algae fermentation 2 fermented by adding a Pseudomonas sp. ATCC 19121 culture solution to 15 wt% aqueous seaweed powder Bacillus subtillus ATCC 4529 cultured in NB medium (foliar spray 10-15 days before harvest), 2% by weight of blood meal, 10% by weight fish meal, 1% by weight glucose, molasses 5 Incubated 5% by weight in a medium consisting of 5% by weight, 0.5% by weight of K ₂ HPO _4, 1% by weight of CSR (Corn Steep Liquor) and the remaining amount of water, and cultured microorganism culture 1 1 after the establishment. Water at intervals and cultured with Bacillus atrophaeus ATCC 7972 cultured in NB broth, 0.03% by weight Beef extract, 0.05% by weight Peptone, soybean scum 10 5% by weight inoculated and cultured in a medium consisting of 1% by weight of glucose, 1% by weight of glucose, 5% by weight of molasses, 0.5% by weight of K ₂ HPO ₄ , 1% by weight of Corn Steep Liquor (CSR) and the remaining amount of water The leaves are sprayed at intervals of 15 to 20 days from the flowering of the flower, and the fruit is greatly increased in weight by cultivating strawberries by irrigating the calcium ionized shell one or two times 15 days before harvest. It was easy to obtain strawberries with high calcium content, high sugar content and improved hardness, and by edible food, there was no need to consume foods that are vitamin C and calcium source, and high sugar content and increased weight By obtaining the fruit, the preference is increased, the hardness is improved, the shelf life is increased, and the marketability is excellent, so that the grower can obtain the effect of improving the added value. It is about a cultivation method.

Strawberry is a fruit of perennial herbaceous plants of the dicotyledonous rose family Rosaceae. The petiole is long and has three relatively large leaves, each rounded and serrated. Several peduncles come out in spring, ranging from a few to dozens of white flowers, with five petals and yellow pistils and stamens. Edible strawberry is different from other fruits that become fruit due to the development of the ovary, the flower jaw is developed. Seeds are not in the fruit and are on the surface of the fruit with sesame seeds.

Fruits are ball-shaped, egg-shaped or oval, usually red, but rarely white varieties. The cultivar was cultivated horticulturally and crossed with several wild species in Europe and the United States. It is from the 17th century that the current strawberry began to be grown.

Wild berries found in Korea include Fragaria nipponica and F. yezoensis of the same genus, while flexible species include Duchesnea charysantha, Potentilla centigrana and Rubus buergeri. ), R. corchorefolius, R. crateagifolius, R. longisepalus, R. phoenicolasius, R. parvifolius, R. idaeus, Beggar Strawberry (R. sorbifolius), bokbunja strawberry (R. coreanus), Hamgyeong strawberry (R. arcticus), berry (R. hirsutus), blackberry (R. croceacantha), berry (R. hongnoensis), strawberry (R. hongnoensis) oldhamii), R. ribesioideus, R. japonicus, and R. palmatus.

Cultivated species include cultivation types such as tactile, semi-tactile, open field, and restraint, and may be cultivated specifically for processing. Tactile cultivation is harvested from December, and special seedlings are grown in high-altitude areas or covered with cold-sand to raise flower buds.

It is usually grown in a plastic house, and it is kept warm by installing a vinyl tunnel inside. They also pick flowers and cover them with bags to pick up large fruits. In the case of semi-promoting cultivation, they are exposed to the cold in winter and dormant.

In restraint cultivation, buds are dug up in late winter, which are not yet open, and are put in the refrigerator, which are planted in early autumn and harvested from autumn to winter. Reproductive varieties may be selected for processing, but the fruit is particularly small, red, and has a good aroma.

The same tree can be harvested every year, but the fruit gradually decreases, and the same field is used continuously. Usually, only the mother tree is left, and the creepage coming out of it is cut and plugged into the seedling to make a new seedling and plant it in the fall. Processing is often left untouched for years.

Strawberry fruit pigment contains a lot of anthocyanin-based ferragin-3-glucoside (18-55mg / live and 100g), as well as gianizine-3-glucoside and beralconidine-3-garacto. It also contains some Saito. The content of sugar is a refractive sugar meter, and the fluctuation range of 5 to 15 degrees is extremely large. In some cases, the temperature may be 17 degrees. Reducing sugars such as glucose and fructose (fructose, glucose) account for about 80%, and there are only a few non-reducing sugars such as sucrose. Acid is citric acid (600-1200mg / 100g juice) and malic acid. Vitamin C is the best (about 80mg / 100g) of common fruit.

Main Ingredients of Strawberry Fruit Moisture (g) Protein (g) Lipid (g) Carbs (g) Mineral (mg) Vitamin C (mg) Sugar fiber Na Ca P Fe 89.1 0.8 0.4 7.1 1.9 2 14 17 0.5 80

Although the fragrance component seems to consist of a carbonyl compound and an acid, the detail is not confirmed. However, the characteristics of the varieties are that the fragrance is felt through the skin and chew strong. It is reported that pectin contains 0.37 to 0.52% (soluble 0.19 to 0.26%, insoluble 0.15 to 0.27%), and constitutes a cell wall and is related to the preservation of strawberry fruit and a suitable ingredient for making jam.

Many vitamin C in strawberries is active in the adrenal cortex that regulates various hormones, so it is effective in improving stamina.

Strawberries have the highest vitamin C content (80mg per 100g), 1.5 times more than tangerines and 10 times more than apples. Six or seven strawberries will help you get all the vitamin C you need. It is often eaten with sugar on strawberries, but it's good to just eat it because it loses the B vitamins it contains to metabolize the body.

On the other hand, when milk or cream is added, citric acid, which is abundant in strawberries, helps the milk absorb calcium and vitamin C is the best in nutritional absorption.

However, in recent years, well-being foods containing a large amount of various components useful for the human body have become popular, but a method of cultivating strawberries to improve the content of other ingredients in addition to the basic ingredients has not been developed.

In particular, vitamin C acts as a reducing agent, helps collagen synthesis, acts as an antioxidant, absorbs iron in the small intestine, is involved in carnitine biosynthesis and immune function, and vitamin C deficiency leads to scurvy and collagen abnormalities. , Bone pain, fractures, diarrhea.

Since vitamin C and calcium are contained in fruits in small amounts, they do not have to eat foods that are vitamin C and calcium sources by simply increasing the content of fruits, and there is a growing method of cultivating strawberries that can improve added value. There is a great demand.

On the other hand, the strawberry has a disadvantage that it is cumbersome to eat small fruit size, if the strawberry pigment is not enough may be confused with immature fruit, there is a problem that the sugar content is not satisfactory in some cases.

In addition, strawberries have a characteristic of falling off with an increase in temperature, especially when the hardness is weak enough to be fingerprinted during harvesting, and when strawberries fall off, there is a lack of commerciality due to lack of marketability, which greatly affects farm income.

Therefore, the object of the present invention was not only to obtain a fruit with a greatly increased weight, but also to easily obtain a strawberry with high vitamin and calcium content, high sugar content and improved hardness. It is not necessary to eat foods that have high sugar content and weight gain. By increasing the preference and hardness, the shelf life is increased, and the shelf life is improved. It is to provide a method of cultivating strawberries with high content and excellent hardness and sugar.

In order to achieve the above objects as well as other objects that can be easily achieved, in the present invention, Bacillus slicheniformis ATCC 102 ( Bacillus slicheniformis ATCC 102) culture solution was added to a 15% by weight aqueous solution of seaweed powder, respectively. Foliar sprinkling at intervals of 15 to 20 days from the flowering of the flower, and fermented algae fermentation broth 2 fermented by adding Pseudomonas sp. ATCC 19121 culture solution to 15 wt% aqueous seaweed powder 10 to 15 days before harvesting Bacillus subtillus ATCC 4529 cultured in NB broth, 2% by weight of blood meal, 10% by weight fish meal, 1% by weight glucose, 5% by weight molasses, K ₂ HPO ₄ 0.5 Inoculate 5% by weight in a medium consisting of 1% by weight, 1% by weight of Corn Steep Liquor (CSR) and the remaining amount of water. , Bacillus atrophaeus ATCC 7972 cultured in NB medium (Nutrient broth), the broth extract (Beef extract) 0.03% by weight, Peptone 0.05% by weight, soybean scum 10% by weight, glucose 5% by weight inoculated in a medium consisting of 1% by weight, 5% by molasses, 0.5% by weight of K ₂ HPO ₄ , 1% by weight of CSR (Corn Steep Liquor) and the remaining amount of water was incubated with each microbial culture 2 Foliar spray at intervals of 15 to 20 days from cultivation of strawberries with a method of irrigation of the ionized calcium agent using a shell once or twice before harvesting 15 days, as well as a largely increased fruit weight as well as vitamin and calcium content This high, high sugar and improved hardness could easily be obtained, and by eating it, it is not necessary to eat foods that are vitamin C and calcium source, and obtain a fruit with high sugar and weight Increased affinity and increased hardness improves the shelf life can be obtained an effect that is superior marketability to be able to enhance the value growers.

By applying the method of the present invention, it is possible to obtain strawberries with a high content of vitamins and calcium, improved sugar and increased weight, and by edible it, there is no need to consume foods that are vitamins and calcium sources, and high sugar and weight By obtaining this increased fruit, the preference increased, the hardness improved, the shelf life increased, and the merchandise was excellent. Thus, the grower was able to improve the added value.

The method for cultivating strawberries with high content of vitamins and calcium, and excellent hardness and sugar, according to the present invention, adds Bacillus licheniformis ATCC 102 ( Bacillus licheniformis ATCC 102) culture solution to a 15% by weight aqueous solution of seaweed fermented seaweed fermentation broth 1 Leaf algae were sprayed at intervals of 15 to 20 days from the flowering of each flower, and the fermented algae fermentation broth 2 fermented by adding Pseudomonas sp. ATCC 19121 culture solution to a 15% by weight aqueous solution of seaweed powder 10 to 15 days before harvesting Bacillus subtillus ATCC 4529 cultured in NB broth, 2% by weight of blood meal, 10% by weight fish meal, 1% by weight glucose, 5% by weight molasses, K ₂ HPO ₄ Inoculate 5% by weight in culture medium consisting of 0.5% by weight, 1% by weight of CSR (Corn Steep Liquor) and the remaining amount of water, and culture the microbial culture solution 1 cultured at regular intervals of 7 to 10 days from the start. Bacillus atrophaeus ATCC 7972 cultured in trient broth) 0.03% by weight of Beef extract, 0.05% by weight of Peptone, 10% by weight of soybean scum, 1% by weight of glucose, 5 to 20% inoculated in medium containing 5% by weight of molasses, 0.5% by weight of K ₂ HPO ₄ , 1% by weight of CSR (Corn Steep Liquor) and the remaining amount of water, and cultured microorganism culture medium 2 from 15 to 20 It is characterized by foliar application at intervals of one day and irrigation of the ionized calcium agent using a shell once or twice before 15 days of harvest.

The seaweed fermentation broth 1 is Bacillus licheniformis ATCC 102 ( Bacillus licheniformis ATCC 102) was inoculated with 2% by weight of the culture solution was prepared by fermentation for 36 hours under the conditions of temperature 30 ℃, stirring speed 100rpm, aeration amount 0.8vvm, pH 7.0.

Bacillus licheniformis ATCC 102 ( Bacillus from above The licheniformis ATCC 102 culture was sterilized by dissolving 3.0 g of Beef extract and 5.0 g of Peptone in 1 L of water, adjusting to pH 7.0, and aseptically inoculating the spawn with platinum. It was incubated with aeration (aeration rate 0.8vvm) at 100rpm.

The seaweed fermentation broth 1 serves as an enlargement promoter for increasing the weight of the strawberry to increase the weight of the strawberry by increasing the number of cells at the time of flowering, fertilization and fruiting.

The seaweed fermentation broth 2 was inoculated with 2 wt% of Pseudomonas sp . ATCC 19121 culture medium in a 15 wt% aqueous solution of seaweed powder, 48 hours under the conditions of temperature 26 ℃, stirring speed 120rpm, aeration rate 1.0vvm, pH 7.0 Prepared by fermentation.

Pseudomonas sp. ATCC 19121 ( Pseudomonas sp . ATCC 19121) The culture broth was dissolved in 1 l of water, 3.0 g of Beef extract, Peptone 5.0 g, 3.0 g of K ₂ HPO ₄ and 2.0 g of MgSO ₄ , adjusted to pH 7.0 and sterilized with spawn seed. Aseptically inoculated and incubated with aeration (aeration amount 1.0vvm) at 120 rpm for 48 hours at 26 ° C.

The seaweed fermentation broth 2 is used for coloring and increasing the vitamin content. In general, when the physiological activity of the crop is vigorous, carbon assimilation is promoted, and as a result, the biosynthesis of sugar is promoted, the coloring proceeds and the vitamin content increases. In the present invention, the color and vitamin content were increased by fermenting the seaweed powder into a microorganism having the ability to produce phenylalanine (Phenylalanine), a biosynthetic precursor of strawberry pigment.

The microbial culture solution 1 was dissolved in 1,000 ml of NB medium (Nutrient broth, Peptone) and Beef Extract (3.0 g) in 1,000 ml of distilled water, adjusted to pH 7.0-7.4 and then sterilized for 15 minutes at 121 °. temperature 30 ℃, stirring speed 100rpm, aeration amount 1.0vvm, 24 hours of incubation a Bacillus subtilis ATCC 4529 (Bacillus subtillus ATCC 4529) blood meal 2% by weight of the medium under the conditions of pH 7.0, 10% by weight of fish meal, glucose to 1% by weight , 5% by weight molasses, K ₂ HPO ₄ 0.5% by weight, CSR (Corn Steep Liquor) 1% by weight and the remaining amount of water inoculated 5% by weight and temperature 30 ℃, stirring speed 100rpm, aeration rate 1.0vvm, pH It was incubated for 24 hours under the conditions of 7.0.

The microbial culture solution 1 is used to improve the sugar content of strawberries to improve sugar content by providing a precursor for biosynthesis of various sugars by fermenting a substrate composed of blood powder, fish meal, and various carbon sources containing animal protein as a microorganism.

The microbial culture 2 is Bacillus atropaeus ATCC 7972 ( Bacillus) incubated for 24 hours under conditions of temperature 30 ℃, stirring speed 100rpm, aeration rate 1.0vvm, pH 7.0 in NB medium (Nutrient broth) atrophaeus ATCC 7972) culture broth extract Beef extract 0.03% by weight, Peptone 0.05% by weight, soybean scum 10% by weight, glucose by 1% by weight, molasses 5% by weight, K ₂ HPO ₄ 0.5% by weight, CSR ( Corn Steep Liquor) 5% by weight inoculated in a medium consisting of 1% by weight and the remaining water and incubated for 24 hours under the conditions of temperature 30 ℃, stirring speed 100rpm, aeration rate 1.0vvm, pH 7.0.

Soybeans contain various substances such as phospholipids, LPE, and 5-ALA, which enhance the function of chlorophyll, which is involved in plant carbon assimilation. Since the hypertrophy is promoted and the biosynthesis of sugars and the pigment is also promoted simultaneously, it exhibits functions such as improving sugar content, promoting coloration, and increasing vitamin content, so that in the present invention, soybean scum (residue left after milking soybean oil) has lipolytic ability. By fermentation and use as a microorganism, it was possible to obtain effects such as improving sugar content, promoting coloring, and increasing vitamin content.

On the other hand, the ionized calcium agent using the shell is fired in a cylindrical firing furnace in which the shell is installed inclined downward and rotated by a driving device and maintained in a high temperature state, the rotation speed of the kiln is 30 ~ 35rpm, shell input 300kg / hr, firing temperature 1,200 ℃ It is prepared so that the calcium content is 99% or more by 45 to 90 minutes in the kiln.

Strawberries have a characteristic of falling off with an increase in temperature, especially when the hardness is weak enough to be fingerprinted during harvesting, and when strawberries fall off, there is a lack of marketability due to lack of marketability, which greatly affects farm incomes. To increase the hardness while improving the calcium content.

The algae fermentation broth 1 prepared as described above was leaf sprinkled at intervals of 15 to 20 days from each flower bloom, and the algae fermentation broth 2 was sprayed 10 to 15 days before harvesting, and the microbial culture solution 1 was fixed 7 to 10 days apart. After irrigation, the microbial culture 2 is sprayed at intervals of 15 to 20 days from each flower bloom, and the ionized calcium agent using a shell is irrigated once or twice 15 days before harvesting.

Particularly, seaweed fermentation broth 1, seaweed fermentation broth 2 and microbial culture 2 are preferably diluted 1,000-fold with water, and microbial culture 1 is diluted with 300 L of water and watered in an area of 660 m 2, and ionized calcium is 700 g of water. It is effective to dilute to 900 L and to water an area of 1,900 m 2.

At this time, the amount of foliar spraying is effective as 300 ~ 1,000ℓ as the final dilution per 300 pyeong, and when the spraying amount is less than 300ℓ, the effect of spreading is not satisfactory, and when it exceeds 1,000ℓ, the excess synergy is weak and economical. Can not do it.

If the dilution ratio is low when used with dilution with water as described above, the growth of microorganisms in the leaves of the strawberry is vigorous and may be damaged. If the dilution ratio is high, the above formulations do not adhere to the leaves and are not satisfactory. The effect is not good.

The following Examples and Experimental Examples illustrate the present invention in more detail, but do not limit the scope of the invention.

Example  1: Preparation of Seaweed Fermentation broth 1

Inoculate 2 wt% of Bacillus licheniformis ATCC 102 culture with 15 wt% aqueous solution of seaweed powder and ferment for 36 hours under conditions of temperature 30 ℃, stirring speed 100rpm, aeration rate 0.8vvm, pH 7.0 Fermentation broth 1 was prepared.

Bacillus licheniformis ATCC 102 ( Bacillus from above The licheniformis ATCC 102 culture was sterilized by dissolving 3.0 g of Beef extract and 5.0 g of Peptone in 1 L of water, adjusting to pH 7.0, and aseptically inoculating the spawn with platinum. It was incubated with aeration (aeration rate 0.8vvm) at 100rpm.

Example  2: Preparation of Seaweed Fermentation Solution 2

Pseudomonas Pseudomonas sp. ATCC 19121 sp . ATCC 19121) 2% by weight of the culture solution was inoculated and fermented under conditions of a temperature of 26 ° C., agitation speed of 120 rpm, aeration rate of 1.0vvm, and pH 7.0 for 48 hours to prepare a seaweed fermentation broth 2.

Pseudomonas sp. ATCC 19121 ( Pseudomonas sp . ATCC 19121) The culture broth was dissolved in 1 l of water, 3.0 g of Beef extract, Peptone 5.0 g, 3.0 g of K ₂ HPO ₄ and 2.0 g of MgSO ₄ , adjusted to pH 7.0 and sterilized with spawn seed. Aseptically inoculated and incubated with aeration (aeration amount 1.0vvm) at 120 rpm for 48 hours at 26 ° C.

Example  3: Preparation of Microbial Culture Solution 1

NB medium (Nutrient broth, Peptone 5.0g and Beef Extract 3.0g dissolved in 1,000ml of distilled water, adjusted to pH 7.0-7.4 and then sterilized at 121 ° for 15 minutes) temperature 30 ℃, stirring Bacillus subtilis ATCC 4529 ( Bacillus) incubated for 24 hours under conditions of 100 rpm, aeration rate 1.0vvm, pH 7.0 subtillus ATCC 4529) culture medium consisting of culture media blood meal 2%, fish meal, 10 wt%, glucose 1%, molasses, 5% by weight, K ₂ HPO ₄ 0.5% by weight, and CSR (Corn Steep Liquor) 1% by weight and the remaining amount of water in the Inoculated at 5% by weight, and incubated for 24 hours under the conditions of temperature 30 ℃, stirring speed 100rpm, aeration rate 1.0vvm, pH 7.0 to prepare a microbial culture 1.

Example  4: Preparation of Microbial Culture Solution 2

Beef extract from Bacillus atrophaeus ATCC 7972 cultures incubated for 24 hours under conditions of temperature 30 ℃, stirring speed 100rpm, aeration rate 1.0vvm, pH 7.0 in NB medium (Nutrient broth) 0.03% by weight, 0.05% by weight of peptone, 10% by weight of soybean scum, 1% by weight of glucose, 5% by weight of molasses, 0.5% by weight of K ₂ HPO ₄ , 1% by weight of Corn Steep Liquor (CSR) and residual water 5% by weight of the inoculated medium was incubated for 24 hours under the conditions of temperature 30 ℃, stirring speed 100rpm, aeration rate 1.0vvm, pH 7.0 to prepare a microbial culture 2.

Example  5: Manufacture of ionized calcium agent

The method described in Patent Application No. 2007-117634, that is, the shell is installed in an inclined downward direction, rotated by a driving device and fired in a cylindrical kiln, maintaining a high temperature state, the rotation speed of the kiln is 30rpm, shell input 300kg / hr, firing temperature An ionized calcium agent having a calcium content of 99% or more was prepared by maintaining it at 1,200 ° C. or higher in a kiln for 65 minutes.

Experimental Example  One

Nonsan No. 3 (Sulhyang) is sprayed with the seaweed fermentation broth 1, the seaweed fermentation broth 2, the microbial culture 1, the microbial culture 2 and the ionized calcium agent prepared in Examples 1 to 5.

That is, the algae fermentation broth 1 was sprayed at intervals of 15 to 20 days from each flower bloom, the algae fermentation 2 was sprayed at 10 to 15 days before harvesting, and the microorganism culture solution 1 was irrigated at intervals of 7 to 10 days after planting. , Microbial culture 2 is sprayed with leaves every 15 to 20 days from the flower bloom and irrigation 1 to 2 times 15 days before harvesting the ionizing calcium agent using a shell.

Particularly, seaweed fermentation broth 1, seaweed fermentation broth 2 and microbial culture 2 are used by diluting 1,000 times with water, and microbial culture 1 is diluted with 300L of water and watered in an area of 660㎡, and ionized calcium agent diluted 700g with 900L of water. Water over an area of 1,900 square meters.

At this time, the amount of foliar spraying was set to 500 l of the final dilution per 300 pyeong.

Strawberries grown in the same manner as described above were harvested and the average weight of 10 was measured. The sugar, calcium content, vitamin C content and hardness were measured by the test methods described in Table 2, and the results are shown in Table 2.

Item No treatment Snow unit Test Methods Sugar 10.5 12.1 Brix ° Refractometer (Use ATAGO (Japan) PAL-2 calcium 13 161 mg / 100g Food Code (2007) Micronutrient Test Vitamin c 82 97 mg / 100g Food Code (2007) Micronutrient Test Hardness 1038.2 1454.1 g / ㎠ Measured with a Fruit Hardness Tester (cat No 9300, Japan) weight 25.1 30.2 g -

As can be seen from Table 2, when the method of the present invention is applied, there are some differences depending on the variety, but it can be seen that the sugar content, calcium content, vitamin C content, hardness, weight are greatly improved.

Claims (3)

Bacillus licheniformis ATCC 102 ( Bacillus licheniformis ATCC 102) culture solution was added to 15% by weight of the seaweed powder, and the algae fermentation broth 1 fermented by foliar spraying at intervals of 15 to 20 days from each flower bloom, and 15% by weight of seaweed powder Bacillus subtillus was cultured in NB medium (Nutrient broth) by foliar spraying of seaweed fermentation broth 2 fermented by adding Pseudomonas sp.ATCC 19121 culture solution to the aqueous solution 10-15 days before harvest ATCC 4529) the culture medium in a medium consisting of 2% by weight blood meal, 10% by weight fish meal, 1% by weight glucose, 5% molasses, 0.5% K ₂ HPO ₄ , 1% by weight of Corn Steep Liquor (CSR) and the balance of water The microbial culture solution 1 inoculated and cultured at 5% by weight was irrigated at intervals of 7 to 10 days after the establishment, and the Bacillus atrophaeus ATCC 7972 cultured in NB medium (Nutrient broth) was used. Beef extract 0.03% by weight, Peptone 0.05%, Soybeans 10%, Glucose 1%, Molasses 5%, K ₂ HPO ₄ 0.5%, CSR (Corn Steep Liquor) 1% Inoculate 5% by weight in a medium consisting of% and the remaining water, and culture the microbial broth 2 incubated at intervals of 15 to 20 days from each flower bloom, and harvest the ionized calcium agent using a shell once or twice a day before harvesting. A method of cultivating strawberries having high vitamin and calcium content and excellent hardness and sugar, which is watering. The method according to claim 1, Bacillus licheniformis ATCC 102 ( Bacillus licheniformis ATCC 102) culture solution Beef extract (Beef extract) and peptone (Peptone) 5.0g is dissolved in 1L of water and sterilized by adjusting to pH 7.0 and spawn A method of cultivating strawberries with high content of vitamins and calcium, and excellent hardness and sugar, characterized by culturing while aseptically inoculated with platinum to aeration (aeration amount of 0.8vvm) at 100 rpm for 30 hours at 30 ° C. The method of claim 1, wherein the Pseudomonas sp. ATCC 19121 culture medium is beef extract (Beef extract) 3.0g, Peptone (5.0g), K ₂ HPO ₄ 3.0g and MgSO ₄ 2.0g in 1L of water Dissolved and adjusted to pH 7.0, sterilized and spawned aseptically with platinum, and cultured with aeration (aeration rate 1.0vvm) at 120rpm for 48 hours at 26 ° C. How to grow strawberries with excellent sugar.