CN103535380B - Crop drought-ressitant agent and application thereof - Google Patents
Crop drought-ressitant agent and application thereof Download PDFInfo
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Abstract
The present invention relates to crop drought-ressitant agent and application thereof, belong to field of crop production.The technical problem to be solved in the present invention is, the crop drought-ressitant agent providing a kind of safety, drought resisting effective and application thereof.Crop drought-ressitant agent of the present invention, its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7 ~ 13 weight portion, betain 1 ~ 3 weight portion, fulvic acid 3 ~ 7 weight portion, organic 1 ~ 3 weight portion, proline quality is 6 ~ 70% of active component gross mass.Meanwhile, the invention also discloses the preparation method and application of crop drought-ressitant agent.The each component of crop drought-ressitant agent of the present invention, all to human body and environmentally friendly, without complicated chemical composition, also can not cause the residual of hormone, can not bring safety problem; There is the advantages such as drought resisting is effective.
Description
Technical field
The present invention relates to crop drought-ressitant agent and application thereof, belong to field of crop production.
Background technology
China is the serious country of a drought and water shortage.Freshwater resources total amount is 28000 billion cubic meters, account for 6% of global water resources, be only second to Brazil, Russia and Canadian, occupy the 4th, the world, but only have 2200 cubic metres per capita, be only 1/4 of world average level, 1/5 of the U.S., ranking 121 in the world, is one of 13 countries that water resource is the poorest per capita in the whole world.Along with the continuous increase of China's population, the fast development of industry and the deterioration of ecotope, China is faced with more serious water shortage problem, and arid seriously governs the raising of China's crop yield.Therefore, in the face of the present situation that growing population pressure and water resource are day by day deficient, study on drought resistance is agriculture important research content always.
Research shows; under drought condition; in plant corpus, osmotic adjustment such as soluble sugar, inorganic ions, free amino acid, proline etc., some Protective enzyme such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) etc., endogenous hormones such as the materials such as abscisic acid (ABA) has increase in various degree; directly or indirectly plant is played regulatory role, strengthen the drought resistance of crop.Can therefore, utilize these materials to improve the drought resistance of crop, moisturizing and coercing lower crop yield be the focus that people pay close attention to always.
Current drought resistance agent technology also has application aborning, more commonly adopts the high molecular polymer with strong absorptive as water-loss reducer, for crop seed soaking or seed dressing, but can only work at crops seedling stage; Also have and adopt antitranspirant, by foliage-spray, make reagent act on pore, to reduce moisture loss, but drought resistance is unstable, also can impact leaf photosynthesis.In recent years, although market has occurred can be used for process in seedling stage, also can be used for the drought resistance agent type that Adult plant sprays, this kind of drought resistance agent mostly is that number of chemical composition is composite to be formed, and complicated component, need to be inquired into, and application cost is higher to the safety of crop; Meanwhile, also have the drought resistance agent adopting plant growth regulator to be composited as gibberellin etc., but somatotropin is residual, result in again new problem.Therefore, finding the drought resistance agent that a kind of drought resisting is truly effective, and can not bring safety problem, is the problem that people thirst for solving always.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of safe and the crop drought-ressitant agent that drought resisting is effective and application thereof.
Crop drought-ressitant agent of the present invention, its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7 ~ 13 weight portion, betain 1 ~ 3 weight portion, fulvic acid 3 ~ 7 weight portion, organic 1 ~ 3 weight portion, proline quality is 6 ~ 70% of active component gross mass.Wherein, compound amino acid has polytype, and raw material mainly divides plant type and animal-type, and plant type compound amino acid, extracts from plant for raw material with natural high-protein plant, is separated several amino acids exquisiteness and forms; Animal-type compound amino acid is with various high protein animal body for raw material, and after fermentable, acid and alkali hydrolysis process, spraying dry processes.Meanwhile, compound amino acid of the present invention also can adopt art methods prepare and obtain: after utilizing peat, brown coal etc. to be separated, then produce after fermentation of becoming thoroughly decomposed, rich amino acids, containing compound amino acids such as cystine, tyrosine, serines.In the present invention, compound amino acid preferably adopts commercially available composite aminophenol powder.Wherein, if not containing proline in compound amino acid, or its proline content is lower than 6% of drought resistance agent active component gross mass, then also need in active component to add proline, makes the quality of proline be 6 ~ 70% of active component gross mass; If reached 6 ~ 70% of active component gross mass containing proline content in compound amino acid, consider from cost-saving angle, then preferably no longer added proline.
Wherein, organic matter refers to the organic water soluble material containing vital functions, as humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid etc.
Further, in order to improve the activity of crop drought-ressitant agent, in addition to the active ingredient (s, also containing auxiliary material in described crop drought-ressitant agent, described auxiliary material can adopt conventional auxiliary material, but considering cost and drought resistance agent activity, described auxiliary material is preferably surfactant and water; Described surfactant can adopt conventional kind, the kinds of surfactants that preferred plant extracts, further preferably Tea Saponin, in Tea Saponin saponin content preferably >=30wt%; Described water is preferably distilled water.
Further, described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid ester betain; The organic at least one be preferably in humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid.
Further, preferably, the active component of crop drought-ressitant agent of the present invention is made up of the component of following weight portion: compound amino acid 10 weight portion, proline-4 weight portion, betain 2 weight portion, fulvic acid 5 weight portion, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan, and described betain is alkyl betaine.
Further, the invention also discloses the preparation method of crop drought-ressitant agent, comprise the steps:
A, according to proportioning weigh compound amino acid, proline, betain, fulvic acid, organic matter and distilled water;
B, first distilled water is inserted in reactor, open to stir and add other components successively, and stir, to obtain final product.
Further, the invention also discloses the application of crop drought-ressitant agent in crop drought resistance, wherein, described crop is preferably vegetables, and namely crop drought-ressitant agent of the present invention can be used for common vegetable crop, is more preferably tomato, celery, asparagus lettuce or cucumber etc.
Further, in crop drought-ressitant agent, active component concentration is too low can not play its drought resisting effect, and excessive concentration then can have an impact to crop, preferably, in use, be diluted with water to betain mass fraction is 0.0006% ~ 0.006% to crop drought-ressitant agent of the present invention; As more preferably scheme, being diluted with water to betain mass fraction is 0.0013% ~ 0.0025%.
Further, in order to improve the drought resisting effect of crop drought-ressitant agent, described application is preferably sprayed on crop plant blade face; Be more preferably when plant seedling stage, diluted 800-1500 and be doubly sprayed on plant blade face, carry out secondary dispenser after being preferable over 10-15 days, the preferred continuous administration of a season crop 2 ~ 3 times, amount of application is 0.04-0.11L/ mu.
Beneficial effect of the present invention:
1, each component of crop drought-ressitant agent of the present invention is all to human body and environmentally friendly, without complicated chemical composition, also can not cause the residual of hormone, can not bring safety problem.
2, crop drought-ressitant agent of the present invention can well improve crop drought resistance ability, is better than commercially available prod.
3, use after each component mixing of the present invention, the function of each component obtains complementation and coordination, the biologic activity of seedling and plant and drought resistance can be made to be organically combined, thus enhance the adaptive capacity of crop to water stress environment, avoid the shortcoming of these materials effect instability when using separately.
4, crop drought-ressitant agent of the present invention obviously can also improve the nutrition condition of crop simultaneously; promote growing and the raising of immunity of crop; strengthen anti-oxidation protection content of material and defence enzyme activity in plant body, improve the ability of crop water suction, moisturizing and removing interior free yl.
Accompanying drawing explanation
Fig. 1 tomato disease index figure;
Fig. 2 tomato state of an illness recovers figure;
Mda content figure in Fig. 3 tomato.
Embodiment
Crop drought-ressitant agent of the present invention, its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7 ~ 13 weight portion, betain 1 ~ 3 weight portion, fulvic acid 3 ~ 7 weight portion, organic 1 ~ 3 weight portion, proline quality is 6 ~ 70% of active component gross mass.Wherein, compound amino acid has polytype, and raw material mainly divides plant type and animal-type, and plant type compound amino acid, extracts from plant for raw material with natural high-protein plant, is separated several amino acids exquisiteness and forms; Animal-type compound amino acid is with various high protein animal body for raw material, and after fermentable, acid and alkali hydrolysis process, spraying dry processes.Meanwhile, compound amino acid of the present invention also can adopt art methods prepare and obtain: after utilizing peat, brown coal etc. to be separated, then produce after fermentation of becoming thoroughly decomposed, rich amino acids, containing compound amino acids such as cystine, tyrosine, serines.In the present invention, compound amino acid preferably adopts commercially available composite aminophenol powder.Wherein, if not containing proline in compound amino acid, or its proline content is lower than 6% of drought resistance agent active component gross mass, then also need in active component to add proline, makes the quality of proline be 6 ~ 70% of active component gross mass; If reached 6 ~ 70% of active component gross mass containing proline content in compound amino acid, consider from cost-saving angle, then preferably no longer added proline.
Wherein, organic matter refers to the organic water soluble material containing vital functions, as humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid etc.
Further, in order to improve the activity of crop drought-ressitant agent, in addition to the active ingredient (s, also containing auxiliary material in described crop drought-ressitant agent, described auxiliary material can adopt conventional auxiliary material, but considering cost and drought resistance agent activity, described auxiliary material is preferably surfactant and water; Described surfactant can adopt conventional kind, the kinds of surfactants that preferred plant extracts, further preferably Tea Saponin, in Tea Saponin saponin content preferably >=30wt%; Described water is preferably distilled water.
Further, described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid ester betain; The organic at least one be preferably in humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid.
Further, preferably, the active component of crop drought-ressitant agent of the present invention is made up of the component of following weight portion: compound amino acid 10 weight portion, proline-4 weight portion, betain 2 weight portion, fulvic acid 5 weight portion, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan, and described betain is alkyl betaine.
Further, the invention also discloses the preparation method of crop drought-ressitant agent, comprise the steps:
A, according to proportioning weigh compound amino acid, proline, betain, fulvic acid, organic matter and distilled water;
B, first distilled water is inserted in reactor, open to stir and add other components successively, and stir, to obtain final product.
Further, the invention also discloses the application of crop drought-ressitant agent in crop drought resistance, wherein, described crop is preferably vegetables, and namely crop drought-ressitant agent of the present invention can be used for common vegetable crop, is more preferably tomato, celery, asparagus lettuce or cucumber etc.
Further, in crop drought-ressitant agent, active component concentration is too low can not play its drought resisting effect, and excessive concentration then can have an impact to crop, preferably, in use, be diluted with water to betain mass fraction is 0.0006% ~ 0.006% to crop drought-ressitant agent of the present invention; As more preferably scheme, being diluted with water to betain mass fraction is 0.0013% ~ 0.0025%.
Further, in order to improve the drought resisting effect of crop drought-ressitant agent, described application is preferably sprayed on crop plant blade face; Be more preferably when plant seedling stage, diluted 800-1500 and be doubly sprayed on plant blade face, carry out secondary dispenser after being preferable over 10-15 days, the preferred continuous administration of a season crop 2 ~ 3 times, amount of application is 0.04-0.11L/ mu.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
The preparation of embodiment 1 crop drought-ressitant agent of the present invention
A, take each component according to proportioning each in table 1;
B, first distilled water is inserted in reactor, open to stir and add other components successively, and stir, to obtain final product.
Table 1 is group drought resistance agent component and proportioning thereof respectively
Table 2 compound amino acid supply producer and article of manufacture lot number
Drought resistance experiment-the tomato of embodiment 2 drought resistance agent of the present invention
1, experiment material:
Experiment crop: tomato seedling, 13 strain × 13 group;
Cultivation matrix: the peat composed of rotten mosses, vermiculite
Experiment reagent: KH-01, KH-02
Contrast agents: Mei Jiafu (manufacturer: Italian tile glug sieve joint-stock company, agent: Jiangmen city Jie Shi agricultural science and technology Co., Ltd, main component is: polypeptide >=380 grams per liter, amino acid >=100 grams per liter, organic nitrogen >=60 grams per liter, (Chelating state) iron+zinc+manganese >=20 grams per liter), the date of manufacture: 20110110)
2, experiment material:
Green house.Each hillslope processes should be placed on the mid portion in greenhouse.The condition uniformity such as the soil texture, pH value, the content of organic matter, fertilizing management situation, planting density of all hillslope processes, and it is actual to meet local agricultural production.
3, experimental technique and design
3.1 agent treated
Reagent proportioning is in table 1, and agent treated situation is in table 3.
The process of table 3 experiment reagent
| Numbering | Experiment process | Extension rate | Amount of application | Spraying time |
| 1-1 | KH-01 | 1000 | 100ml | Before arid |
| 1-2 | KH-01 | 1000 | 100ml | After arid |
| 2-1 | KH-01 | 500 | 100ml | Before arid |
| 2-2 | KH-01 | 500 | 100ml | After arid |
| 3-1 | KH-02 | 1000 | 100ml | Before arid |
| 3-2 | KH-02 | 1000 | 100ml | After arid |
| 4-1 | KH-02 | 500 | 100ml | Before arid |
| 4-2 | KH-02 | 500 | 100ml | After arid |
| 5-1 | Mei Jiafu | 1000 | 100ml | Before arid |
| 5-2 | Mei Jiafu | 1000 | 100ml | After arid |
| 6-1 | CK1 | Clear water | 100ml | Before arid |
| 6-2 | CK2 | Clear water | 100ml | After arid |
| 7 | CK3 | Clear water | 100ml | Without Osmotic treatment |
3.2 experimental design
8 process (5 contrasts) are established in this test, often process 1 time and repeat, amount to 13 communities.Each community randomized arrangement, each community 13 basin tomato, tomato hillslope processes design table is in table 4.
Table 4 tomato hillslope processes design table
4 investigation, record, measuring method
4.1 meteorological and soil informations
The record intensity of illumination of experimental session and temperature (daily mean temperature, the highest and minimum temperature, with DEG C represent).Data should from nearest meteorological station or preferably at sample plot record.
The data such as record soil types, soil fertility, soil moisture content, weeds coverage condition.
4.2, investigation method
Arid 2(Extreme drought respectively in experimentation), (recovering after Extreme drought) two periods of watering carry out 2 investigation.
When the evaluation of tomato seedling Identification of Drought is not sought unity of standard, this experimental basis tomato plant Extreme drought and water recover after Symptoms formulated respectively seedling stage damage caused by a drought and Extreme drought recover grade scale.
Seedling stage damage caused by a drought grade scale:
0 grade---seedling is normal, without any symptom;
1 grade---young leaves is dim, and siphonal lobe is obviously wilted;
3 grades---young leaves is obviously wilted, and siphonal lobe is seriously wilted, and stem is upright;
5 grades---blade is seriously wilted, stem lodging or half lodging;
7 grades---plant is seriously wilted dehydration, and stem lodges.
Wherein 5 grades, 7 grades is Extreme drought symptom.
Extreme drought recovers grade scale:
0 grade---seedling recovers normal completely, without any arid injury symptom;
1 grade---seedling recovers normal substantially, and bottom 1-2 sheet leaf shows obvious wilting;
3 grades---cane is upright, and growing point has vigor, and blade is seriously wilted and is difficult to recover normal, and plant, is subject to grievous injury;
5 grades---plant is seriously wilted death, irrecoverable.
Auxiliary characteristics can be surveyed: leaf area change, rhizome ratio.
4.3 measuring method
Respectively in above-mentioned 2 investigation, gather blade, measure blade malonaldehyde accumulation content.
Arid and plant membrane injure close relation.Arid can cause the damage of cell membrane system, can cause plant cell death time serious.Superoxide dismutase (SOD) is the main protective enzyme of Lipid peroxidation metabolism system of defense, and it catalytic activity oxygen generation disproportionated reaction can produce nontoxic molecular oxygen and hydrogen peroxide, thus avoids plant to sustain an injury.Catalase (CAT) and peroxidase (POD) are all the protective enzymes in Lipid peroxidation metabolism system of defense, can remove the H in physiological system
2o
2.One of primary product that malonaldehyde (MDA) is Lipid peroxidation metabolism, MDA content under drought stress raises with the increase of Middle altitude mountain, Solute accumulation can be there is in plant in inducing cell, the flow of water is reduced by osmotic adjustment, thus cell turgor is maintained when ensureing to organize the flow of water to decline, that is, mda content is lower, and its drought resistance is better.The osmotic adjustment of plant realizes mainly through the accumulation of compatibility solute.Thus crop drought resistance sexual state can be inferred by measuring mda content.
5 Analysis of conclusions
5.1 experimental result
Arid 2(Extreme drought in experimentation) time investigate original disease index, disease index investigation raw data table 5 shown in, original disease index figure is as shown in Figure 1.
Table 5 tomato disease index
| Community | 0 grade | 1 grade | 3 grades | 5 grades | 7 grades | Disease index/% |
| 6-2 | 1 | 8 | 4 | 40.77 | ||
| 6-1 | 2 | 8 | 2 | 1 | 33.08 | |
| 5-2 | 2 | 10 | 1 | 30.00 | ||
| 5-1 | 2 | 11 | 26.92 | |||
| 4-2 | 2 | 11 | 26.92 | |||
| 3-2 | 4 | 8 | 1 | 25.38 | ||
| 2-2 | 5 | 7 | 1 | 23.85 | ||
| 4-1 | 4 | 9 | 23.85 | |||
| 3-1 | 6 | 7 | 20.77 | |||
| 2-1 | 7 | 6 | 19.23 |
| 1-2 | 7 | 6 | 19.23 | |||
| 1-1 | 8 | 5 | 17.69 | |||
| 7 | 13 | 0 |
Disease index (R) is calculated as follows:
R=（N
0*0+N
1*0.1+N
3*0.3+N
5*0.5+N
7*0.7）/n*100%
Wherein, N
0, N
1, N
3be respectively the tomato seedling number that grade is 0 grade, 1 grade, 3 grades etc., n is total seedling number.
Arid 2(Extreme drought in experimentation) after water, and observe after 2d recovery situation and measure mda content, after 2d, disease index is in table 6, the state of an illness recover figure as shown in Figure 2; Mda content is in table 7, and mda content figure as shown in Figure 3.
Table 6 tomato state of an illness index of recovery
| Community | 0 grade | 1 grade | 3 grades | 5 grades | Disease index/% |
| 6-2 | 3 | 7 | 37.61 | ||
| 6-1 | 1 | 5 | 3 | 31.62 | |
| 5-2 | 4 | 5 | 2 | 28.21 | |
| 5-1 | 10 | 25.64 | |||
| 4-2 | 10 | 25.64 | |||
| 3-2 | 1 | 7 | 1 | 24.79 | |
| 2-2 | 2 | 8 | 22.22 | ||
| 4-1 | 3 | 7 | 20.51 | ||
| 3-1 | 5 | 5 | 17.09 | ||
| 2-1 | 6 | 4 | 15.38 | ||
| 1-2 | 6 | 4 | 15.38 | ||
| 1-1 | 7 | 3 | 13.68 | ||
| 7 | 13 | 0 |
Mda content in table 7 tomato
| Community | Mda content | Appearance character |
| 6-2 | 0.06390 | Wilting seedling |
| 6-1 | 0.06130 | Wilting seedling |
| 5-2 | 0.05124 | Wilting seedling |
| 5-1 | 0.04929 | Wilting seedling |
| 4-2 | 0.04600 | Wilting seedling |
| 3-2 | 0.03857 | Wilting seedling |
| 2-2 | 0.03804 | Wilting seedling |
| 4-1 | 0.03568 | Wilting seedling |
| 3-1 | 0.03530 | Wilting seedling |
| 2-1 | 0.03455 | Wilting seedling |
| 1-2 | 0.03235 | Wilting seedling |
| 1-1 | 0.03086 | Wilting seedling |
| 7 | 0.02699 | Plant is normal |
According to Fig. 1 and table 5,6-2(CK2, water after drought) and 6-1(CK1, water before drought) to compare, 6-1 disease index is lower, waters than better effects if of watering after drought before therefore can finding out drought, and meanwhile, each experimental group also demonstrates this conclusion; Meanwhile, rank the first be 1-1(KH-01-1000 ×, drought before spray medicine).
Analyze according to Fig. 2 and table 6,1-1 disease index is minimum, and be also that effect is best, namely its recovery situation is best.
Analyze according to Fig. 3 and table 7, that mda content is the highest is 6-2(CK1, waters after drought), coercing that the tomato seedling of that is watering after drought is subject to is maximum, and this result and the first two result are coincide.
To sum up, compared with contrast agents, 1-1 all shows obvious otherness, and successful is excellent in competing product, and namely KH-01-1000 × effect is better.
5.2 Analysis of conclusion
The tomato seedling disease index this time watered before experiment drought and mda content are all lower than watering after drought, this all shows except uniformity accurately in various medicament, simultaneously, water before KH-01 reagent drought with water after drought all in same process performance consistent and effect is best, and drought resistance agent effect of the present invention is better than contrast agents, demonstrates reagent drought resisting of the present invention further and there is good drought resisting effect.
Drought resistance experiment-the asparagus lettuce of embodiment 3 drought resistance agent of the present invention
1 experiment material:
Experiment crop: asparagus lettuce, 9 strain × 10 group;
Experiment reagent: KH-07, KH-08
Contrast agents: western Mg(A-MicsurMg, Probelte company of Spain produces, batch number: FECHA06/06/2011LOTE11060134)
2 test sites
Green house, indoor temperature maintains 12-28 DEG C.Each experimental plot should be placed on the mid portion in greenhouse.The condition uniformity such as the soil texture, pH value, the content of organic matter, fertilizing management situation, planting density of all experimental plots, and it is actual to meet local agricultural production.
3 test methods and design
3.1 reagent names and concentration
Reagent proportioning is in table 1, and the concentration of reagent is in table 8.
Table 8 reagent and concentration process
| Numbering | Reagent | Extension rate | Amount of application | Plot area |
| 1 | KH-07 | 800 | 80ml | Each community 9 basin |
| 2 | KH-07 | 1500 | 80ml | Each community 9 basin |
| 3 | KH-08 | 800 | 80ml | Each community 9 basin |
| 4 | KH-08 | 1500 | 80ml | Each community 9 basin |
| 5 | Western Mg | 800 | 80ml | Each community 9 basin |
| 6 | Western Mg | 1500 | 80ml | Each community 9 basin |
| 7 | CK | Normally water | 80ml | Each community 9 basin |
| 8 | CK | Arid | 80ml | Each community 9 basin |
3.2 hillslope processes designs
8 process (2 contrasts) are established in this test, often process 1 time and repeat, amount to 10 communities.Each community randomized arrangement,
Each community 9 basin asparagus lettuce, experimental plot design table is in table 9.
Community, table 9 asparagus lettuce seedbed distribution table
4 test methods
4.1 asparagus lettuce process and spray medicine
Asparagus lettuce size uniformity is chosen in test, and carries out dispenser without bad upgrowth situation.
4.2 investigation method
Arid 2(Extreme drought respectively in experimentation), (recovering after Extreme drought) two periods of watering carry out 2 investigation.
When asparagus lettuce drought resistance of seedling characterization and evaluation is not sought unity of standard, this test basis asparagus lettuce plant Extreme drought and water recover after Symptoms formulated respectively seedling stage damage caused by a drought and Extreme drought recover grade scale, grade scale is with embodiment 1.
4.3 measuring method
Respectively in above-mentioned 2 investigation, gather blade, measure blade malonaldehyde accumulation content.
The processing method of Extreme drought is adopted to carry out Evaluation of drought resistance to it, to determine that tomato seedling uses the change of drought resistance after preparation.
5 test results and analysis
5.1 result of the test
Data survey is in table 10:
The different reagent of table 10 is on the impact of asparagus lettuce Drought resistance rank
Shown by table 10 result, with contrast CK(arid) compared with, different reagent different disposal has remarkable result to asparagus lettuce drought-resistant ability.Wherein, KH-07-1500, KH-08-800, KH-08-1500 are the strongest to asparagus lettuce drought-resistant ability, and drought symptom is partially light.
After 7d, test mda content in different disposal group asparagus lettuce, often organize Stochastic choice three strain asparagus lettuce, mda content the results are shown in Table 11.
Mda content in table 11 different disposal group asparagus lettuce
5.3 Analysis of conclusion
Table 10 result shows, with contrast CK(arid) compared with, different reagent different disposal has remarkable result to asparagus lettuce drought-resistant ability.Wherein, KH-07-1500, KH-08-800, KH-08-1500 are the strongest to asparagus lettuce drought-resistant ability, and drought symptom is partially light.
Table 11 result shows, with contrast CK(arid) compared with, in different its asparagus lettuce of agent treated, mda content all has reduction, wherein, KH-07, KH-08 process reduces significantly, especially KH-07-1500 ×, KH-08-800 ×, KH-08-1500 ×, with contrast ratio, all there is significant difference, Be very effective.
6, experiment conclusion
Comprehensive different reagent and concentration are on the impact of asparagus lettuce Drought resistance rank, and different reagent is on the impact of mda content in asparagus lettuce, drought resistance agent formula KH-08 is under 800 and 1500 times of effects, to the drought resisting effect of asparagus lettuce compared with the control, effect is the most obvious, that is, drought resistance agent of the present invention has good drought resistance, is better than commercially available prod.
Drought resistance experiment-the celery of embodiment 4 drought resistance agent of the present invention
1 test material
Trial crops: celery 90 strain, 9 strain × 10 group.
1, experiment material:
Experiment crop: celery
Experiment reagent: KH-07, KH-08
Contrast agents: claim western Mg(A-MicsurMg, Probelte company of Spain produces, batch number: FECHA06/06/2011LOTE11060134)
2 test sites
Green house, indoor temperature maintains 12-28 DEG C.Each experimental plot should be placed on the mid portion in greenhouse.The condition uniformity such as the soil texture, pH value, the content of organic matter, fertilizing management situation, planting density of all experimental plots, and it is actual to meet local agricultural production.
3 experimental scheme
3.1 reagent names and concentration
Reagent proportioning is in table 1, and the concentration of reagent is in table 12.
Table 12 reagent and concentration process
| Numbering | Reagent | Extension rate | Amount of application | Plot area |
| 1 | KH-07 | 800 | 80ml | Each community 9 basin |
| 2 | KH-07 | 1500 | 80ml | Each community 9 basin |
| 3 | KH-08 | 800 | 80ml | Each community 9 basin |
| 4 | KH-08 | 1500 | 80ml | Each community 9 basin |
| 5 | Western Mg | 800 | 80ml | Each community 9 basin |
| 6 | Western Mg | 1500 | 80ml | Each community 9 basin |
| 7 | CK | Normally water | 80ml | Each community 9 basin |
| 8 | CK | Arid | 80ml | Each community 9 basin |
3.2 Quarter Design
8 process (2 contrasts) are established in this test, often process 1 time and repeat, amount to 10 communities.Each community randomized arrangement, each community 9 basin celery, hillslope processes distribution table is in table 13.
Community, table 13 seedbed distribution table
4 test methods
4.1 celery process and spray medicine
Celery size uniformity is chosen in test, and carries out dispenser without bad upgrowth situation.
4.2 investigation method
Arid 2(Extreme drought respectively in experimentation), (recovering after Extreme drought) two periods of watering carry out 2 investigation.
When the evaluation of celery Identification of Drought in seedling stage is not sought unity of standard, this test basis celery plant Extreme drought and water recover after Symptoms formulated respectively seedling stage damage caused by a drought and Extreme drought recover grade scale, grade scale is with embodiment 1.
4.3 measuring method
If investigation method can not be successful, detect the change of inclusion.Respectively in above-mentioned 2 investigation, gather blade, measure blade malonaldehyde accumulation content.
The processing method of Extreme drought is adopted to carry out Evaluation of drought resistance to it, to determine that celery to use the change of drought resistance after preparation seedling stage.
5 test results and analysis
5.1 result of the test
Respectively at the laggard line number of dispenser 5d according to investigations, data survey is in table 14 in this test:
The different reagent of table 14 is on the impact of celery Drought resistance rank
Table 14 result shows, with contrast CK(arid) compared with, different reagent different disposal has remarkable result to celery drought-resistant ability.
After 5d, test mda content in different disposal group celery, often organize Stochastic choice three strain celery, mda content the results are shown in Table 15.
Table 15 different disposal is on the impact of celery mda content
Table 15 result shows, with contrast CK(arid) compared with, in different its celery of reagent concentration process, mda content all has remarkable reduction, and wherein, KH-08-800 × and KH-08-1500 × process significantly reduce, and have pole significant difference, Be very effective.
5.2 Analysis of conclusion
Table 14 result shows, with contrast CK(arid) compared with, different reagent different disposal has remarkable result to celery drought-resistant ability.Wherein, KH-08 is the strongest to celery drought-resistant ability, and drought symptom is partially light.
Table 15 result shows, with contrast CK(arid) compared with, in its celery of different agent treated, mda content all has reduction, and wherein, KH-08 process reduces significantly, with contrast ratio, all has pole significant difference, Be very effective.
6, conclusion (of pressure testing)
Comprehensive different reagent and concentration are on the impact of celery Drought resistance rank, and different reagent is on the impact of mda content in celery, and to the drought resisting effect of celery compared with the control, effect is the most obvious for drought resistance agent formula KH-08; Meanwhile, the drought resisting effect of drought resistance agent of the present invention all shows excellence, and drought resistance is better than commercially available prod.
Drought resistance experiment-the cucumber of embodiment 5 drought resistance agent of the present invention
1 test material
Trial crops: cucumber 90 strain, 9 strain × 10 group.
1, experiment material:
Experiment crop: cucumber
Experiment reagent: KH-03, KH-04
Contrast agents: western Mg(A-MicsurMg, Probelte company of Spain produces, batch number: FECHA06/06/2011LOTE11060134)
2 test sites
Green house, indoor temperature maintains 12-28 DEG C.Each experimental plot should be placed on the mid portion in greenhouse.The condition uniformity such as the soil texture, pH value, the content of organic matter, fertilizing management situation, planting density of all experimental plots, and it is actual to meet local agricultural production.
3 experimental scheme
3.1 reagent names and concentration
Reagent proportioning is in table 1, and the concentration of reagent is in table 16.
Table 16 reagent and concentration process
| Numbering | Reagent | Extension rate | Amount of application | Plot area |
| 1 | KH-03 | 800 | 90ml | Each community 9 basin |
| 2 | KH-03 | 1500 | 90ml | Each community 9 basin |
| 3 | KH-04 | 800 | 90ml | Each community 9 basin |
| 4 | KH-04 | 1500 | 90ml | Each community 9 basin |
| 5 | Western Mg | 800 | 90ml | Each community 9 basin |
| 6 | Western Mg | 1500 | 90ml | Each community 9 basin |
| 7 | CK | Normally water | 90ml | Each community 9 basin |
| 8 | CK | Arid | 90ml | Each community 9 basin |
3.2 Quarter Design
8 process (2 contrasts) are established in this test, often process 1 time and repeat, amount to 10 communities.Each community randomized arrangement, each community 9 basin cucumber, hillslope processes distribution table is in table 17.
Community, table 17 seedbed distribution table
4 test methods
4.1 cucumber process and spray medicine
Cucumber size uniformity is chosen in test, and carries out dispenser without bad upgrowth situation.
4.2 investigation method
Arid 2(Extreme drought respectively in experimentation), (recovering after Extreme drought) two periods of watering carry out 2 investigation.
When the evaluation of cucumber at seedling stage Identification of Drought is not sought unity of standard, this test basis cucumber plant Extreme drought and water recover after Symptoms formulated respectively seedling stage damage caused by a drought and Extreme drought recover grade scale, grade scale is with embodiment 1.
4.3 measuring method
Respectively in above-mentioned 2 investigation, gather blade, measure blade malonaldehyde accumulation content.
The processing method of Extreme drought is adopted to carry out Evaluation of drought resistance to it, to determine that celery to use the change of drought resistance after preparation seedling stage.
5 test results and analysis
5.1 result of the test
Respectively at the laggard line number of dispenser 5d according to investigations, data survey is in table 18 in this test:
The different reagent of table 18 is on the impact of cucumber Drought resistance rank
Table 18 result shows, with contrast CK(arid) compared with, different reagent different disposal has remarkable result to cucumber drought-resistant ability.
After 5d, test mda content in different disposal group cucumber, often organize Stochastic choice three strain cucumber, mda content the results are shown in Table 19.
Table 19 different disposal is on the impact of cucumber mda content
Table 19 result shows, with contrast CK(arid) compared with, in different its cucumber of reagent concentration process, mda content all has remarkable reduction, and wherein, KH-04-800 × and KH-04-1500 × process significantly reduce, and have pole significant difference, Be very effective.
5.2 Analysis of conclusion
Table 18 result shows, with contrast CK(arid) compared with, different reagent different disposal has remarkable result to cucumber drought-resistant ability.Wherein, KH-04 is the strongest to cucumber drought-resistant ability, and drought symptom is partially light.
Table 19 result shows, with contrast CK(arid) compared with, in its cucumber of different agent treated, mda content all has reduction, and wherein, KH-04 process reduces significantly, with contrast ratio, all has pole significant difference, Be very effective.
6, conclusion (of pressure testing)
Comprehensive different reagent and concentration are on the impact of cucumber Drought resistance rank, and different reagent is on the impact of mda content in cucumber, and to the drought resisting effect of cucumber compared with the control, effect is the most obvious for drought resistance agent formula KH-04; Meanwhile, the drought resisting effect of drought resistance agent of the present invention all shows excellence, and drought resistance is better than commercially available prod.
Drought resistance experiment-other Vegetables of embodiment 6 drought resistance agent of the present invention
Adopt KH-05, KH-06, and adopt the method for embodiment 5 also to carry out drought resistance experiment to the Vegetables such as romaine lettuce, capsicum, experimental result all shows, the drought resistance of crop drought-ressitant agent of the present invention is excellent, is better than commercially available prod.
Claims (15)
1. crop drought-ressitant agent, is characterized in that: its active component is compound amino acid, betain, fulvic acid, organic matter and proline; Wherein, compound amino acid 7 ~ 13 weight portion, betain 1 ~ 3 weight portion, fulvic acid 3 ~ 7 weight portion, organic 1 ~ 3 weight portion, proline quality is 6 ~ 70% of active component gross mass; Wherein, described organic matter is at least one in humus, chitin derivativ, alginic acid, plant polyose, mushroom polysaccharide, vegetable alkaloid.
2. crop drought-ressitant agent according to claim 1, is characterized in that: in addition to the active ingredient (s, and also containing auxiliary material in described crop drought-ressitant agent, described auxiliary material is surfactant and water.
3. crop drought-ressitant agent according to claim 2, is characterized in that: described surfactant is Tea Saponin, saponin >=30wt% in described Tea Saponin.
4. crop drought-ressitant agent according to claim 1 and 2, is characterized in that: described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid ester betain.
5. crop drought-ressitant agent according to claim 3, is characterized in that: described betain is at least one in alkyl betaine, alkyl amido betaine, azochlorosulfonate propyl lycine, alkyl azochlorosulfonate propyl lycine, phosphoric acid ester betain.
6. crop drought-ressitant agent according to claim 4, is characterized in that: its active component is made up of the component of following weight portion: compound amino acid 10 weight portion, proline-4 weight portion, betain 2 weight portion, fulvic acid 5 weight portion, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan; Described betain is alkyl betaine.
7. crop drought-ressitant agent according to claim 5, is characterized in that: its active component is made up of the component of following weight portion: compound amino acid 10 weight portion, proline-4 weight portion, betain 2 weight portion, fulvic acid 5 weight portion, organic 3 weight portions; Wherein, described organic matter is chitin derivativ and lentinan; Described betain is alkyl betaine.
8. the application of any one of claim 1 ~ 7 crop drought-ressitant agent in crop drought resistance.
9. the application of crop drought-ressitant agent according to claim 8 in crop drought resistance, is characterized in that: described crop is vegetables.
10. the application of crop drought-ressitant agent according to claim 9 in crop drought resistance, is characterized in that: described crop tomato, celery, asparagus lettuce or cucumber.
The application of the crop drought-ressitant agent described in 11. according to Claim 8 ~ 10 any one, is characterized in that: during application, and being diluted with water to betain mass fraction is 0.0006% ~ 0.006%.
The application of 12. crop drought-ressitant agents according to claim 11, is characterized in that: during application, and being diluted with water to betain mass fraction is 0.0013% ~ 0.0025%.
The application of the crop drought-ressitant agent described in 13. according to Claim 8 ~ 10 any one, is characterized in that: described in be applied as and be sprayed on crop plant blade face.
The application of 14. crop drought-ressitant agents according to claim 11, is characterized in that: described in be applied as and be sprayed on crop plant blade face.
The application of 15. crop drought-ressitant agents according to claim 12, is characterized in that: described in be applied as and be sprayed on crop plant blade face.
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| CN106900743A (en) * | 2017-02-25 | 2017-06-30 | 韩贺男 | The compound formulation of corn growth under a kind of promotion drought stress |
| CN107156191A (en) * | 2017-05-14 | 2017-09-15 | 吴刚 | A kind of preparation method of crops drought resistance agent |
| CN108456055A (en) * | 2018-02-09 | 2018-08-28 | 河南科技学院 | A kind of arabidopsis drought resistance agent, preparation method and application method |
| CN108903044B (en) * | 2018-09-10 | 2020-12-22 | 云南省烟草农业科学研究院 | Flue-cured tobacco management and baking method for relieving gray tobacco based on betaine application |
| CN109329305A (en) * | 2018-09-29 | 2019-02-15 | 江苏农林职业技术学院 | Tillandsia copper ion drought resistance agent and the preparation method and application thereof |
| CN109329301A (en) * | 2018-09-29 | 2019-02-15 | 江苏农林职业技术学院 | Tillandsia drought resistance agent and its preparation method and application |
| CN109757247B (en) * | 2019-03-19 | 2021-07-06 | 中国农业科学院烟草研究所 | Application of porphyra polysaccharide in improving drought resistance of plants |
| CN111990412B (en) * | 2020-07-21 | 2021-09-03 | 广东拉多美化肥有限公司 | Humic acid composite plant high-temperature-resistant protective agent and preparation method thereof |
| CN114916557A (en) * | 2022-05-07 | 2022-08-19 | 渭南东旺农华生物科技有限公司 | Agricultural composition containing lecithin and gamma-aminobutyric acid |
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