CN106900743A - The compound formulation of corn growth under a kind of promotion drought stress - Google Patents
The compound formulation of corn growth under a kind of promotion drought stress Download PDFInfo
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- CN106900743A CN106900743A CN201710105016.2A CN201710105016A CN106900743A CN 106900743 A CN106900743 A CN 106900743A CN 201710105016 A CN201710105016 A CN 201710105016A CN 106900743 A CN106900743 A CN 106900743A
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- corn
- drought stress
- ala
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of compound formulation of corn growth under promotion drought stress, the concentration of its composition for including and each composition is：5 10~100mg/L of amino-laevulic acid；General 14.9~the 149mg/L that receives of NO donor nitre；117~2340mg/L of glycine betaine.The compound formulation can strengthen the drought-resistant ability of corn, processing corn seed with certain density compound formulation can improve Course of Corn Seed Germination rate under drought stress, improves seedling growth, alleviate the injury that drought stress is caused to corn growth, and then improve the yield of corn under drought stress.
Description
Technical field
Specifically it is exactly corn life under a kind of promotion drought stress the present invention relates to the degeneration-resistant preparation technique field of crops
Compound formulation long.
Background technology
At present, during Course of Corn Seed Germination, drought stress can have a strong impact on the sprouting of seed, have to later growth
Strong influence.With the change of global climate environment, China's various regions Dry land rate increase influences sprouting and raw for corn
It is long, cause the different degrees of underproduction.Corn is the important cereal crops of China, but weather conditions during northern China corn seeding
General poor, drought and water shortage phenomenon is serious, and thus the germination percentage of corn seed is just relatively low, and its seedling growth is also weaker, from
And finally influence corn yield.
Research shows, 5-ALA（5-ALA）The synthesis of chlorophyll can be adjusted, chlorophyll and photosystem is improved
II stability, improves photosynthetic efficiency, promotes photosynthesis, promotes the basic physiologies such as plant tissue differentiation activity, therefore it is simultaneously
It is not merely a kind of biological metabolism intermediate product, the regulation process that involved in plant grows is gone back, with similar plants hormone
Physiologically active, can use as plant growth regulator in agricultural production.
NO is the Molecular regulator of plant growth and development, and biological and abiotic adverse circumstance can be reacted, and
It is also a kind of important semiochemicals in the Defense response reaction of plant.Influences and plant hormone of the NO to plant growing cycle
There is close relationship.NO is in seed sprouting, de-etiolation, the growth of root, the stretching, extension of leaf, the formation of flower and fruit maturation and declines
Played an important role in old.Plant is often subject to the stress of biotic stress and abiotic stress under field conditions (factors).Plant has one
The complete defense mechanism of set, NO plays an important role in biotic stress as signaling molecule.And as the sodium nitroprussiate of NO donors
(SNP) NO of abundance can be provided for plant, and then regulates and controls growing for plant.
Glycine betaine（GB）Solubility it is very high, without electrostatic charge in the range of certain physiology PH, the glycine betaine of high concentration（GB）
There is no damage effect to many enzymes and other biological macromolecular, or even have protective effect.Studying more than 150 kinds of metabolin
In, glycine betaine（GB）It is best Osmolyte regulator.Under normal condition, glycine betaine in plant（GB）Content it is not high, work as plant
When thing is subject to environment stress, synthesizing betaine in cell（GB）Pass build enzyme betaine-aldehyde dehydrogenase activity rising, in vivo it is sweet
Dish alkali（GB）Content increases sharply, and the glycine betaine in plant leaf blade（GB）Concentration is closely related with water osmotic potential, table
Bright glycine betaine（GB）Serve good osmotic adjustment effect.In addition, glycine betaine（GB）Adverse environmental factor air holes is moved,
Respiration and related gene expression have certain regulating and controlling effect.So being imposed to corn seed under drought stress and seedling outer
Source glycine betaine（GB）, can also certain influence be produced to its physiological status.
The content of the invention
Research shows, certain density 5-ALA（5-ALA）, nitre Pu Na（SNP）And glycine betaine（GB）Can be with
Strengthen the drought-resistant ability of corn, use certain density 5-ALA（5-ALA）, nitre Pu Na（SNP）, glycine betaine（GB）Place
Reason corn seed is improving Course of Corn Seed Germination rate under drought stress, improve seedling growth, to alleviate drought stress to jade
The injury that meter Sheng Chang is caused, and then improve the yield of corn under drought stress.
The present invention is directed to deficiency of the prior art, it is therefore an objective to provides and a kind of promotes the compound of corn growth under drought stress
Preparation.
The present invention solve the technical scheme that is used of above-mentioned technical problem for：
The compound formulation of corn growth under a kind of promotion drought stress, it is characterised in that：Its composition for including and each composition it is dense
Spend and be：
5-ALA（5-ALA）10~100mg/L；Nitre Pu Na（SNP）14.9~149mg/L；Glycine betaine（GB）117~
2340mg/L。
Preferably, the concentration of the compound formulation, its composition for including and each composition is：
5-ALA（5-ALA）25~75mg/L；Nitre Pu Na（SNP）14.9~74.5mg/L；Glycine betaine（GB）117~
585mg/L。
Preferably, the concentration of the compound formulation, its composition for including and each composition is：
5-ALA（5-ALA）50mg/L；Nitre Pu Na（SNP）29.8mg/L；Glycine betaine（GB）292.5mg/L.
The device have the advantages that being：
PEG-6000 in experiment of the invention using 14% carries out drought stress, has probed into the 5- glycyls third of various concentrations
Acid（5-ALA）, nitre Pu Na（SNP）And glycine betaine（GB）Influence to Course of Corn Seed Germination under drought stress and seedling growth, examination
Checking is bright, and these three allogenic materials grow with adjustment effect to corn under drought stress；Compared to drought stress bar
The treatment of external source mixed liquor is not added with part, with the external source 5-ALA of various concentrations（5-ALA）, nitre Pu Na（SNP）With it is sweet
Dish alkali（GB）After mixed liquor treatment corn seedling, each group Chlorophyll-a Content has rising, and seed germination rate is also raised, peroxidating
Thing enzyme（POD）Activity increase；It is 50mg/L 5-ALAs with concentration（5-ALA）, concentration be 29.8mg/L nitre Pu Na
（SNP）It is 292.5mg/L glycine betaines with concentration（GB）Mixed liquor Course of Corn Seed Germination under drought stress and seedling growth are made
With most pronounced effects, seed germination rate is up to 90%, POD activity and reaches 5120 Ug-1FW·min-1, Chlorophyll-a Content reaches
6.983 mg/g·FW。
Specific embodiment
In order to be more clearly understood to technical characteristic of the invention, purpose, effect and embodiment, this hair is now illustrated
Bright specific embodiment.
The concentration of the compound formulation of corn growth under a kind of promotion drought stress, its composition for including and each composition is：
5-ALA（5-ALA）10~100mg/L, nitre Pu Na（SNP）14.9~149mg/L, glycine betaine（GB）117~
2340mg/L。
Embodiment 1：
The concentration of the compound formulation, its composition for including and each composition is：
5-ALA（5-ALA）50mg/L, nitre Pu Na（SNP）29.8mg/L, glycine betaine（GB）292.5mg/L.
It is on the method that Course of Corn Seed Germination and growth of seedling influence using the compound formulation under test drought stress：
（1）100 stalwartnesses, corn seeds full, of the same size are chosen, is sterilized with the NaClO that mass concentration is 10g/L
20min, then with distilled water flushing three times, then blotted with blotting paper；
（2）Three layers of gauze and three metafiltration paper, the filter paper that this 100 corn seeds are placed in culture dish on culture dish middle berth
On, to the water 25ml that 25 DEG C are added in culture dish, it is placed on 25 DEG C of constant temperature culture 24h in incubator；
（3）After 14%PEG-6000 solution 15ml soaking corn seeds 12h is added in culture dish, 10ml is added by this implementation
The 5-ALA of example proportioning mixing（5-ALA）, nitre Pu Na（SNP）And glycine betaine（GB）Intermixture；
（4）Seed sprouting number is counted after 15 days, germination percentage is calculated；
（5）After 30 days, the consistent corn seedling of growing way that will be treated through 14%PEG-6000 is moved into flowerpot, per one plant of basin, warp
One week seedling-slowing stage is crossed, then drought stress is carried out with 14%PEG-6000, with the 5-ALA mixed by said ratio（5-
ALA）, nitre Pu Na（SNP）And glycine betaine（GB）Intermixture carry out three foliage-sprays, the interval time for spraying every time is 6h,
Daily to the moisture that loses is supplemented in flowerpot, a Hoagland's nutrient solution was poured every three days；
（6）The corn seedling leaf of each basin is taken out under dark conditions, is shredded, mixed, weigh fresh sample 0.1g, sample is placed in mortar
It is interior, a small amount of magnesium carbonate and quartz sand are added, the acetone grinding homogenate of 5ml is added, then add the acetone 10ml of volumetric concentration 85% to continue to grind
Tissue white is milled to, be transferred to homogenate in the volumetric flask of 25ml by the funnel through being covered with filter paper, and with third that volumetric concentration is 80%
Ketone cleans mortar and filter paper by several times, is ginseng with the acetone that volumetric concentration is 80% finally with the acetone constant volume that volumetric concentration is 80%
Than liquid, light absorption value is determined at 663nm and 645nm wavelength to determine the content of chlorophyll a；
（7）Peroxidase is measured using guaiacol method（POD）Activity：Leaf in the middle of the plant of 0.1g is weighed to be put into mortar,
It is phosphate buffer that 0.05 mol/L, pH is 5.5 as extract solution using concentration, first adds 1mL extract solutions, grinds 1min, then
9 mL extract solutions are added, homogenate is all transferred in centrifuge tube, in the bar that centrifuge speed is 4000 r/min, temperature is 4 DEG C
10 min are centrifuged under part, the supernatant for the obtaining i.e. crude extract of enzyme determines peroxidase（POD）When active, by 2mL phosphoric acid
Buffer solution, 1mL peroxide enzyme liquid, 1mL concentration are the guaiacol and H that 1mL volumetric concentrations are 2% of 0.05mol/L2O2Mixing
Liquid shakes up and pours into cuvette rapidly immediately in adding test tube, in using U2800 type UV, visible light spectrophotometrics under 470nm wavelength
Meter colorimetric, records absorbance at once.
Embodiment 2：
The composition that includes of compound formulation and the concentration of each composition that the present embodiment is selected be：5-ALA（5-ALA）
10mg/L, nitre Pu Na（SNP）14.9mg/L, glycine betaine（GB）117mg/L.
Implement using the compound formulation is same on the method that Course of Corn Seed Germination and growth of seedling influence under test drought stress
Example 1.
Embodiment 3：
The composition that includes of compound formulation and the concentration of each composition that the present embodiment is selected be：5-ALA（5-ALA）
25mg/L, nitre Pu Na（SNP）74.5mg/L, glycine betaine（GB）585mg/L.
Implement using the compound formulation is same on the method that Course of Corn Seed Germination and growth of seedling influence under test drought stress
Example 1.
Embodiment 4：
The composition that includes of compound formulation and the concentration of each composition that the present embodiment is selected be：5-ALA（5-ALA）
75mg/L, nitre Pu Na（SNP）149mg/L, glycine betaine（GB）1170mg/L.
Implement using the compound formulation is same on the method that Course of Corn Seed Germination and growth of seedling influence under test drought stress
Example 1.
Embodiment 5：
The composition that includes of compound formulation and the concentration of each composition that the present embodiment is selected be：5-ALA（5-ALA）
100mg/L, nitre Pu Na（SNP）298mg/L, glycine betaine（GB）2340mg/L.
Implement using the compound formulation is same on the method that Course of Corn Seed Germination and growth of seedling influence under test drought stress
Example 1.
Comparative example 1：
Do not add any reagent to process corn seed and corn seedling, Course of Corn Seed Germination and seedling are given birth under test drought stress
The method of influence long is with embodiment 1.
Comparative example 2：
Any reagent is not added to process corn seed and corn seedling, to Course of Corn Seed Germination and children under test moisture sufficiency
The method of seedling growth effect is with embodiment 1.
Measure result of the test such as following table：
PEG-6000 in experiment of the invention using 14% carries out drought stress, has probed into the 5- glycyls third of various concentrations
Acid（5-ALA）, nitre Pu Na（SNP）And glycine betaine（GB）Influence to Course of Corn Seed Germination under drought stress and seedling growth, examination
Checking is bright, and these three allogenic materials grow with adjustment effect to corn under drought stress.
Compared to the treatment of external source mixed liquor is not added with drought stress conditions, with the external source 5-ALA of various concentrations
（5-ALA）, nitre Pu Na（SNP）And glycine betaine（GB）After mixed liquor treatment corn seedling, each group Chlorophyll-a Content has rising,
Seed germination rate is also raised, peroxidase（POD）Activity increase.
Above-mentioned experiment shows：It is 50mg/L 5-ALAs with concentration（5-ALA）, concentration be 29.8mg/L nitre it is general
Receive（SNP）It is 292.5mg/L glycine betaines with concentration（GB）Mixed liquor to Course of Corn Seed Germination under drought stress and seedling growth
Action effect is most notable, and seed germination rate is up to 90%, POD activity and reaches 5120 Ug-1FW·min-1, Chlorophyll-a Content reaches
6.983 mg/g·FW。
Claims (3)
1. it is a kind of promote drought stress under corn growth compound formulation, it is characterised in that：Its composition for including and each composition
Concentration is：
10~100mg/L of 5-ALA；
General 14.9~the 149mg/L that receives of NO donor nitre；
117~2340mg/L of glycine betaine.
2. under a kind of promotion drought stress according to claims 1 corn growth compound formulation, it is characterised in that：Its
Including composition and the concentration of each composition be：
25~75mg/L of 5-ALA；
General 14.9~the 74.5mg/L that receives of NO donor nitre；
117~585mg/L of glycine betaine.
3. under a kind of promotion drought stress according to claims 1 or 2 corn growth compound formulation, its feature exists
In：The concentration of its composition for including and each composition is：
5-ALA 50mg/L；
The general 29.8mg/L that receives of NO donor nitre；
Glycine betaine 292.5mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710105016.2A CN106900743A (en) | 2017-02-25 | 2017-02-25 | The compound formulation of corn growth under a kind of promotion drought stress |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710105016.2A CN106900743A (en) | 2017-02-25 | 2017-02-25 | The compound formulation of corn growth under a kind of promotion drought stress |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110157628A (en) * | 2018-03-15 | 2019-08-23 | 湖南省植物保护研究所 | It is a kind of improve Genes For Plant Tolerance drought stress ability and promote plant growth photosynthetic bacteria PSB-1 microbial inoculum and its application |
| CN115968883A (en) * | 2022-12-07 | 2023-04-18 | 郑州郑氏化工产品有限公司 | Composition containing 5-aminolevulinic acid and nicotinamide ester and application thereof |
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| CN115968883A (en) * | 2022-12-07 | 2023-04-18 | 郑州郑氏化工产品有限公司 | Composition containing 5-aminolevulinic acid and nicotinamide ester and application thereof |
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Application publication date: 20170630 |