CN109329305A - Tillandsia copper ion drought resistance agent and the preparation method and application thereof - Google Patents
Tillandsia copper ion drought resistance agent and the preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention provides a kind of Tillandsia copper ion drought resistance agent, the raw material components including following concentration: concentration is the Cu of 80~280mg/L2+Solution.Tillandsia copper ion drought resistance agent of the invention has good drought resisting effect to Tillandsia, and Tillandsia wilting coefficient reaches 19.1%, declines 52% compared with the control group；Relative conductivity reaches 19.8%, declines 67.4% compared with the control group, and water content reaches 67.5%, increases 66.7% compared with the control group.Meanwhile the preparation method of the drought resistance agent is simple and effective, is not required to add any industrial chemicals, is applied to that Tillandsia is without causing harm, and will not influence the physicochemical property of soil.
Description
Technical field
The present invention relates to drought resistance agent with and the preparation method and application thereof more particularly to a kind of Tillandsia copper ion drought resistance agent
And the preparation method and application thereof.
Background technique
Tillandsia (scientific name Tillandsia, English name Air plant) is pineapple family (Bromeliaceae) Tillandsia
(Tillandsia) herbaceos perennial, including nearly 550 kinds and 90 mutation, it is various in style, come in every shape.China is
Just start to introduce this plant in recent years.Because (in air, when cultivation is not required to mud to the exclusive feature of Tillandsia plant for growth
Soil, and have the characteristics that absorb the harmful substances such as formaldehyde, heavy metal), just liked and paid close attention to by many people once introducing.It is empty
Gas pineapple is under the jurisdiction of pineapple family (Bromeliaceae), is monocotyledon, perennial herb.Pineapple family has 3 subfamilies, 50
Belong to, more than 2600 kinds.3 subfamilies are respectively Honoka pineapple subfamily (Pitcairnioideae), iron orchid subfamily
(Tillandsioideae) and pineapple subfamily (Bromelioideae).Most Tillandsias are iron orchid subfamily Tillandsias
(Tillandsia) plant.Tillandsia is a maximum category in pineapple family, has a initial species more than 500, is distributed in Latin America sea
Pull out the broad regions of 1000~3000m.In primitive environment, they grows nonparasitically upon another plant or hangs on trunk or stone, even cable
On line.Currently, drought resistance agent used in China is starch grafted acrylate class drought resistance agent, belong to one kind of water-retaining agent, technique is multiple
Miscellaneous, higher cost is not suitable for large area and uses, and largely using the physicochemical property that can influence soil in various degree, and not
The drought resistance agent for being suitable as home flower uses.
Summary of the invention
Goal of the invention: the first object of the present invention is to provide a kind of Tillandsia survival rate and growth quality of effectively improving
Drought resistance agent；The second object of the present invention is to provide the preparation method of the Tillandsia copper ion drought resistance agent；Third mesh of the invention
The application for being to provide the Tillandsia copper ion drought resistance agent.
Technical solution: the present invention provides a kind of Tillandsia copper ion drought resistance agent, the raw material components including following concentration: dense
Degree is the Cu of 80~280mg/L2+Solution.
Preferably, Cu2+Solution is copper sulphate and/or copper chloride；Cu2+Solution concentration is 100~250mg/L.
Further, the Tillandsia copper ion drought resistance agent further includes the raw material components of following concentration: potassium chloride concentration
300~500mg/L, 150~200mg/L of concentration of proline, 15~20mg/L of 6-BA concentration, 10~15mg/L of beet alkali concentration,
5~12mg/L of gibberellin concentration, 10~20g/L of sucrose concentration, nitrogen concentration be 80~120mg/L.
Preferably, the nitrogen is the pure urea of chemistry.
Preferably, wherein 6-BA is 6- benzyl aminoadenine, and alias: 6-benzyl aminopurine, the basic element of cell division, English are logical
With name: 6-Benzylaminopurine, molecular formula: C12H11N5。
The preparation method of the Tillandsia copper ion drought resistance agent, includes the following steps:
(1) aqueous solution of both the ethanol solution of proline, the hydrochloric acid solution of 6-BA and gibberellin and glycine betaine is stirred
It mixes and is mixed and made into auxiliary material solution；
(2) aqueous solution of potassium chloride, urea, sucrose three are added in auxiliary material solution, just miscible fluid is made in stirring；
(3) by Cu2+Solution is added in just miscible fluid, is stirred and is adjusted pH value, Tillandsia copper ion drought resisting is made
Agent.
Wherein, it is 40~60 DEG C that middle whipping temp is stirred described in step (1), and mixing time is 2~3h.
Preferably, the ethanol solution of the proline is to be dissolved in proline in 95% ethanol solution, and whipping temp is
20~30 DEG C, mixing time is made for 0.5~1h.
Further, the hydrochloric acid solution of 6-BA is that 6-BA is dissolved in concentration to be in the hydrochloric acid solution of 1mol/L, temperature when dissolution
Degree is 40~60 DEG C, and mixing time is made for 1~2h.
Preferably, the aqueous solution of both gibberellin and glycine betaine is gibberellin, glycine betaine difference is soluble in water, dissolution temperature
Degree is 30~35 DEG C, and mixing time is that 1~2h is made.
Preferably, in step (2) described stirring, whipping temp is 45~65 DEG C, and mixing time is 2~4h.
Wherein, potassium chloride, urea, the aqueous solution Ei of sucrose three are soluble in water by potassium chloride, urea, sucrose difference, by three kinds
Aqueous solution is stirred, and whipping temp is 50~60 DEG C, mixing time is 2~3h.
Further, step (3) is described stirs and adjusts in pH value, and whipping temp is 30~45 DEG C, mixing time 3
~5h, and adjusting pH value is 5.7~6.5.
Wherein, step (3) Cu2+Solution is that the stirring soluble in water of the pure copper sulphate of chemistry is made.
The application of the Tillandsia copper ion drought resistance agent, wherein application Tillandsia copper ion drought resistance agent is to impregnate
30~40min of Tillandsia plant.
Preferably, Tillandsia copper ion is applied in application of the Tillandsia copper ion drought resistance agent in Tillandsia drought resisting
Temperature is controlled when drought resistance agent at 25~30 DEG C.
Further, application of the Tillandsia copper ion drought resistance agent in Tillandsia drought resisting, application Tillandsia copper from
Humidity when sub- drought resistance agent is 75~90%；Control shading rate is 50~60% when applying Tillandsia copper ion drought resistance agent.
The utility model has the advantages that Tillandsia copper ion drought resistance agent provided by the invention, Tillandsia is imitated with good drought resisting
Fruit, Tillandsia wilting coefficient reach 19.1%, decline 52% compared with the control group；Relative conductivity reaches 19.8%, and right
According to group compared to decline 67.4%, water content reaches 67.5%, increases 66.7% compared with the control group.Meanwhile the system of the drought resistance agent
Preparation Method is simple and effective, is not required to add any industrial chemicals, and it is without causing harm to be applied to Tillandsia, and will not shadow
Ring the physicochemical property of soil.
Specific embodiment
It is described further below for technical solution of the present invention.
The invention will be further described below, and from Shanghai, Chinese medicines group chemical reagent has raw material of the invention with reagent
Limit company buys.
Embodiment 1
The preparation and application of Tillandsia copper ion drought resistance agent
(1) proline is dissolved in 95% ethanol solution, whipping temp is 20 DEG C, mixing time 0.5h；By 6-BA
It is dissolved in the hydrochloric acid solution that concentration is 1mol/L, temperature is 40 DEG C when dissolution, mixing time 1h；By gibberellin, glycine betaine point
Not soluble in water, solution temperature is 30 DEG C, and mixing time is 1h；By the ethanol solution of proline, 6-BA hydrochloric acid solution,
The aqueous solution of gibberellin and the aqueous solution of glycine betaine are mixed and made into auxiliary material solution, and whipping temp is 40 DEG C when mixing, stirring
Time is 2h.
(2) by potassium chloride, urea, sucrose difference it is soluble in water, three kinds of aqueous solutions are stirred, whipping temp be 50 DEG C,
Mixing time is 2h, this mixed aqueous solution is added in auxiliary material solution, and just miscible fluid, whipping temp 45 is made in stirring
DEG C, mixing time 2h.
(3) copper sulphate is soluble in water, and by this Cu2+Aqueous solution is added in just miscible fluid, is sufficiently stirred and air is made
Pineapple copper ion drought resistance agent, whipping temp are 30 DEG C, mixing time 3h, and adjusting pH value is 5.7, and each ingredient is dense in solution
Degree is respectively Cu2+Concentration 80mg/L, potassium chloride concentration 300mg/L, concentration of proline 150mg/L, 6-BA concentration 15mg/L, beet
Alkali concentration 10mg/L, gibberellin concentration 5mg/L, sucrose concentration 10g/L, nitrogen concentration be 80mg/L.
Tillandsia plant 35min is impregnated using Tillandsia copper ion drought resistance agent, plant is taken out later, is placed in environment
Temperature is 28 DEG C, during which carries out conventional cultivating and managing to Tillandsia.The result shows that drought resisting effect is good.
Embodiment 2
The preparation and application of Tillandsia copper ion drought resistance agent
(1) proline is dissolved in 95% ethanol solution, whipping temp is 30 DEG C, mixing time 1h；6-BA is molten
In the hydrochloric acid solution that concentration is 1mol/L, temperature is 60 DEG C when dissolution, mixing time 2h；Gibberellin, glycine betaine are distinguished
Soluble in water, solution temperature is 35 DEG C, and mixing time is 2h；By the ethanol solution of proline, the hydrochloric acid solution, red of 6-BA
The aqueous solution of mycin and the aqueous solution of glycine betaine are mixed and made into auxiliary material solution, and whipping temp is 60 DEG C when mixing, when stirring
Between be 3h.
(2) by potassium chloride, urea, sucrose difference it is soluble in water, three kinds of aqueous solutions are stirred, whipping temp be 60 DEG C,
Mixing time is 3h, this mixed aqueous solution is added in auxiliary material solution, and just miscible fluid, whipping temp 65 is made in stirring
DEG C, mixing time 4h.
(3) copper sulphate or copper chloride is soluble in water, and by this Cu2+Aqueous solution is added in just miscible fluid, is sufficiently stirred
Be made Tillandsia copper ion drought resistance agent, whipping temp is 45 DEG C, mixing time 5h, and adjusting pH value is 6.5, in solution respectively at
The concentration divided is respectively Cu2+Concentration 280mg/L, potassium chloride concentration 500mg/L, concentration of proline 200mg/L, 6-BA concentration
20mg/L, beet alkali concentration 15mg/L, gibberellin concentration 12mg/L, sucrose concentration 20g/L, nitrogen concentration be 120mg/L.
Tillandsia plant 35min is impregnated using Tillandsia copper ion drought resistance agent, plant is taken out later, is placed in environment
Temperature is 28 DEG C, during which carries out conventional cultivating and managing to Tillandsia.The result shows that drought resisting effect is good.
Embodiment 3
The preparation and application of Tillandsia copper ion drought resistance agent
(1) proline is dissolved in 95% ethanol solution, whipping temp is 25 DEG C, mixing time 0.75h；By 6-BA
It is dissolved in the hydrochloric acid solution that concentration is 1mol/L, temperature is 50 DEG C when dissolution, mixing time 1.5h；By gibberellin, glycine betaine
Soluble in water respectively, solution temperature is 33 DEG C, and mixing time is 1.5h；By the ethanol solution of proline, the hydrochloric acid of 6-BA
The aqueous solution of solution, the aqueous solution of gibberellin and glycine betaine is mixed and made into auxiliary material solution, and whipping temp is 50 DEG C when mixing,
Mixing time is 2.5h.
(2) by potassium chloride, urea, sucrose difference it is soluble in water, three kinds of aqueous solutions are stirred, whipping temp be 55 DEG C,
Mixing time is 2.5h, this mixed aqueous solution is added in auxiliary material solution, and stirring is made just miscible fluid, whipping temp and is
55 DEG C, mixing time 3h.
(3) copper sulphate or copper chloride is soluble in water, and by this Cu2+Solution is added in just miscible fluid, and system is sufficiently stirred
At Tillandsia copper ion drought resistance agent, whipping temp is 35 DEG C, mixing time 4h, and adjusting pH value is 6.0, in solution respectively at
The concentration divided is respectively Cu2+Concentration 150mg/L, potassium chloride concentration 400mg/L, concentration of proline 175mg/L, 6-BA concentration
18mg/L, beet alkali concentration 13mg/L, gibberellin concentration 10mg/L, sucrose concentration 15g/L, nitrogen concentration be 100mg/L.
Tillandsia plant 35min is impregnated using Tillandsia copper ion drought resistance agent, plant is taken out later, is placed in environment
Temperature is 28 DEG C, during which carries out conventional cultivating and managing to Tillandsia.The result shows that drought resisting effect is good.
Embodiment 4
The concentration of Tillandsia copper ion drought resistance agent primary raw material component selects
(1) experimental group model construction
Each Tillandsia kind selects the almost the same plant of size, the short leaf of dead leaf around plant is wiped out, in greenhouse
Middle recovery carries out drought stress after half a month.Set 18 DEG C of temperature control, blower, sunshade net in the greenhouse, summer shading rate is 50~60%,
Equipment, the air humiditys 75~90% such as cascade, micro- spray are divulged information daily.Drought stress: Tillandsia plant is placed in outdoor outdoor
Place carries out drought stress processing, herein without shade, full exposure；The method of drought stress processing is the 15d that do not water continuously, in arid
There is no rainwater during stress.Daily morning 8:00-8:30, noon 14:00-14:30 and 17:00-17 in afternoon during drought stress:
30 measurement outdoor temperatures and intensity of illumination, outdoor day samming during counting drought stress are as follows: 26.7 DEG C, daytime per day light
According to intensity are as follows: 65264lux.
Tillandsia plant is placed in greenhouse (greenhouse experiment is same as above) water spray and moistened completely to plant after drought stress and is
It is quasi-.Tillandsia copper ion drought resistance agent is prepared with raw material components according to the step of embodiment 3 within second day, wherein Cu2+Solution is to change
Learn pure CuSO4·5H2The solution that O is made into, Cu2+Concentration is set as 0,50,80,100,150,200,250,280,300,350mg/L,
Wherein 0mg/L is control (as water), totally 10 processing.Each processing sets 3 repetitions, and each each kind of repetition is growth one
20 plants of the Tillandsia plant of cause.
Each processing Cu of respective concentration2+Solution impregnates Tillandsia plant 0.5h, later takes out plant, is placed in greenhouse
It is interior.Routine Management is carried out to Tillandsia during test.Tillandsia is from Tillandsia copper ion drought resistance agent treated third
It is primary that it starts water spray daily, and water spray standard is subject to plant is completely wet, water spraying time 8:00-9:00 or afternoon 4 in the morning:
00-6:00；It is primary that the Tillandsia special fertilizer that N P and K proportion is 20:10:20 is sprayed weekly, and spraying concentration is dilution 1000-
1500 times.
(2) measurement item
The upgrowth situation that each processing Tillandsia is observed in treatment process, records its wilting plant number, calculates wilting coefficient.
Wilting coefficient=processing of death plant number * 100/ plant number.
Processing measures the conductivity of each processing Tillandsia after 1 month.Specific determination step is as follows: respectively handling each repetition
5 plants are randomly selected, the acquisition leaf of falling 2-3 (counts 2-3 leaf from lobus cardiacus) outward, measures turbine blades in electrochemical matter leaching rate with conductivity gauge method.Phase
Rear conductivity is boiled to conductivity (%)=original conductivity * 100/.
Processing measures the leaf r elative water content of each processing Tillandsia after 1 month.It is measured and is counted using weighting method after dried
Calculate leaf r elative water content.Leaf r elative water content=(Wf-Wd) * 100/ (Wt-Wd), Wf is fresh weight in formula, and Wd is leaf
Piece dry weight, Wt are that blade is saturated fresh weight.
(3) interpretation of result
Influence of the 3.1 Tillandsia copper ion drought resistance agents processing to Tillandsia wilting coefficient after drought stress
Test plant material is after drought stress with different Cu2+The Tillandsia copper ion drought resistance agent of solution concentration carries out
Processing, its wilting coefficient is different within 1 month, is shown in Table 1.
The wilting coefficient of 1 Tillandsia of table (wilting coefficient unit is %)
| Cu2+Solution concentration (mg/L) | Dimension road orange red is received | Elfin | Si Chuikete | Bake is vertical |
| 0 | 42.8 | 48.9 | 40.2 | 44.1 |
| 50 | 40.3 | 46.6 | 39.5 | 42.8 |
| 80 | 32.4 | 34.6 | 29.8 | 33.8 |
| 100 | 23.8 | 25.1 | 21.5 | 22.7 |
| 150 | 25.7 | 25.8 | 24.0 | 20.8 |
| 200 | 20.2 | 22.5 | 19.1 | 21.6 |
| 250 | 20.6 | 23.6 | 20.8 | 21.2 |
| 280 | 30.4 | 32.7 | 31.6 | 33.2 |
| 300 | 41.0 | 46.2 | 39.0 | 42.5 |
| 350 | 39.2 | 45.2 | 39.5 | 41.4 |
This research is reflected by wilting coefficient applies Tillandsia copper ion drought resistance agent to Tillandsia under drought stress
Plant survives situation, and wilting coefficient is lower, and plant survival rate is lower；Conversely, Tillandsia survival rate is higher.As shown in Table 1,
For trying in Tillandsia kind, Cu2+When concentration is 50mg/L, 300mg/L and 350mg/L, wilting coefficient difference compared with the control
It is not significant, illustrate Cu2+It is unobvious to the wilting coefficient for reducing Tillandsia when concentration is too low and excessively high, that is, it is unfavorable for improving empty
The drought resistance of gas pineapple；Work as Cu2+When concentration is 80mg/L, 100mg/L, 150mg/L, 200mg/L, 250mg/L and 280mg/L,
The wilting coefficient of Tillandsia, which is substantially less than, to be compareed, and illustrates to apply 80~280mg/L Cu outside2+When solution, the arid side of body can effectively reduce
Tillandsia wilting coefficient after compeling especially applies 100-250mg/LCu when outer2+When solution, sky after drought stress can be significantly reduced
Gas pineapple wilting coefficient, significantly improves its survival rate, to improve its drought resistance.Therefore Cu2+ excessive concentration too low is unfavorable for
The reduction of Tillandsia wilting coefficient and plant recovery growth after drought stress.
Influence of the 3.2 Tillandsia copper ion drought resistance agents processing to Tillandsia relative conductivity after drought stress
The relative conductivity of 2 Tillandsia plant of table (relative conductivity unit is %)
With the relative conductivity of electrical conductivity method measurement plant leaf blade, relative conductivity can reflect the strong of the drought resistance of plant
It is weak.When drought stress occurs, blade cell film is easy to be damaged, and membrane permeability increases, and cell inclusion extravasation leads to outer sepage
Conductivity increases, and injury of plasmalemmae degree increases, and drought resistance weakens.Relative conductivity is higher, and the drought resistance of plant is lower；It is relatively electric
Conductance is lower, illustrates that the drought resistance of plant is higher.By measuring different Cu2+The relative conductivity of each kind, determines copper under concentration
Influence of the ion concentration to the drought resistance for improving Tillandsia.
The relative conductivity of Tillandsia plant is shown in Table 2 after the processing of Tillandsia copper ion drought resistance agent.As shown in Table 2, it supplies
It tries in Tillandsia kind, Cu in Tillandsia copper ion drought resistance agent2+When concentration is 50mg/L, 300mg/L and 350mg/L, phase
To conductivity, difference is not significant compared with the control, higher, illustrates Cu2+Concentration it is too low and it is excessively high be unfavorable for improve Tillandsia
Drought resistance；Work as Cu2+When concentration is 80mg/L, 100mg/L, 150mg/L, 200mg/L, 250mg/L and 280mg/L, air phoenix
The relative conductivity of pears, which is substantially less than, to be compareed, and illustrates to apply 80~280mg/L Cu outside2+When solution, Tillandsia can be effectively improved
Drought resistance especially applies 100~250mg/LCu when outer2+When solution, it is remarkably improved the drought resistance of Tillandsia.
Influence of the 3.3 Tillandsia copper ion drought resistance agents processing to Tillandsia leaf r elative water content after drought stress
3 Tillandsia leaf r elative water content of table (leaf r elative water content unit is %)
| Cu2+Solution concentration (mg/L) | Dimension road orange red is received | Elfin | Si Chuikete | Bake is vertical |
| 0 | 39.8 | 40.7 | 40.5 | 38.6 |
| 50 | 43.1 | 45.6 | 44.3 | 45.2 |
| 80 | 57.6 | 58.2 | 58.0 | 59.3 |
| 100 | 65.4 | 65.2 | 66.0 | 63.1 |
| 150 | 65.7 | 63.8 | 64.2 | 62.5 |
| 200 | 63.6 | 64.4 | 66.1 | 60.3 |
| 250 | 65.8 | 64.7 | 67.5 | 62.2 |
| 280 | 56.4 | 57.8 | 57.4 | 56.1 |
| 300 | 45.5 | 44.2 | 41.0 | 42.4 |
| 350 | 45.2 | 46.0 | 43.8 | 43.7 |
Moisture is the basis for maintaining the effect of plant normal physiological, and leaf r elative water content can reflect plant internal water
Wane degree, and Under Drought Stress in Plant leaf r elative water content is higher, and water retention capability is stronger, and plant drought resistance is stronger.
Different Cu2+The leaf r elative water content of Tillandsia is shown in Table 3 after the Tillandsia copper ion drought resistance agent processing of concentration.
As shown in Table 3, for trying in Tillandsia kind, Cu2+When concentration is 50mg/L, 300mg/L and 350mg/L, compared with the control, leaf
Piece relative water content difference is not significant, lower, illustrates Cu2+Excessive concentration or it is too low when, to improve Tillandsia blade phase
It is unobvious to water content effect, it is unfavorable for improving the drought resistance of Tillandsia；Work as Cu2+Concentration be 80mg/L, 100mg/L,
When 150mg/L, 200mg/L, 250mg/L and 280mg/L, the leaf r elative water content of Tillandsia is significantly higher than control and other
Processing illustrates to apply 80~280mg/L Cu outside2+When solution, the drought resistance of Tillandsia can be effectively improved, especially, is applied when outside
100~250mg/LCu2+When solution, it is remarkably improved the drought resistance of Tillandsia.
Three comprehensive wilting coefficient, conductivity and relative water content indexs, the arid side of body are concluded that by this research
After compeling, certain density Cu is used2+The drought resistance of Tillandsia, Cu can be improved in solution2+It is too high or too low to be unfavorable for improving
Tillandsia drought resistance applies 80~280mg/L Cu outside2+When solution, the drought resistance of Tillandsia can be effectively improved, especially, when
100~250mg/LCu is applied outside2+When solution, it is remarkably improved the drought resistance of Tillandsia.
Comparative example 1
Influence of the proline to Tillandsia copper ion drought resistance agent effect
It is added without proline, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3
Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 38.8%, relative conductivity is according to the method for embodiment 4
58.5%, relative water content 42.6%.Difference is not significant compared with the control for each testing index, illustrates to be added without proline
The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.
Comparative example 2
Influence of the potassium chloride to Tillandsia copper ion drought resistance agent effect
It is added without potassium chloride, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3
Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 39.2%, relative conductivity is according to the method for embodiment 4
60.0%, relative water content 40.5%.Difference is not significant compared with the control for each testing index, illustrates to be added without potassium chloride
The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.
Comparative example 3
Influence of the 6-BA to Tillandsia copper ion drought resistance agent effect
It is added without 6-BA, remaining prepares Tillandsia copper ion drought resistance agent according to the method and raw material components of embodiment 3,
According to the method for embodiment 4, measure that the vertical wilting coefficient in Tillandsia kind Bake is 40.0%, relative conductivity is
59.8%, relative water content 41.7%.Difference is not significant compared with the control for each testing index, illustrates to be added without 6-BA not
The vertical drought resistance in Tillandsia kind Bake can significantly be improved.
Comparative example 4
Influence of the glycine betaine to Tillandsia copper ion drought resistance agent effect
It is added without glycine betaine, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3
Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 43.2%, relative conductivity is according to the method for embodiment 4
61.5%, relative water content 40.8%.Difference is not significant compared with the control for each testing index, illustrates to be added without glycine betaine
The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.
Comparative example 5
Influence of the gibberellin to Tillandsia copper ion drought resistance agent effect
It is added without gibberellin, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3
Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 41.3%, relative conductivity is according to the method for embodiment 4
58.6%, relative water content 42.5%.Difference is not significant compared with the control for each testing index, illustrates to be added without gibberellin
The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.
Comparative example 6
Influence of the nitrogen to Tillandsia copper ion drought resistance agent effect
It is added without nitrogen, remaining prepares Tillandsia copper ion drought resistance agent according to the method and raw material components of embodiment 3,
According to the method for embodiment 4, measure that the vertical wilting coefficient in Tillandsia kind Bake is 43.6%, relative conductivity is
62.4%, relative water content 39.5%.Difference is not significant compared with the control for each testing index, illustrates to be added without nitrogen not
The vertical drought resistance in Tillandsia kind Bake can significantly be improved.
Claims (9)
1. a kind of Tillandsia copper ion drought resistance agent, which is characterized in that the raw material components including following concentration: concentration be 80~
The Cu of 280mg/L2+Solution.
2. Tillandsia copper ion drought resistance agent according to claim 1, it is characterised in that: Cu2+Solution concentration be 100~
250mg/L；Cu2+Solution is copper sulphate.
3. Tillandsia copper ion drought resistance agent according to claim 1 or 2, which is characterized in that further include following concentration
Raw material components: 300~500mg/L of potassium chloride concentration, 150~200mg/L of concentration of proline, 15~20mg/L of 6-BA concentration, sweet tea
10~15mg/L of dish alkali concentration, 5~12mg/L of gibberellin concentration, 10~20g/L of sucrose concentration, nitrogen concentration be 80~
120mg/L。
4. the preparation method of Tillandsia copper ion drought resistance agent as claimed in claim 3, which comprises the steps of:
(1) aqueous solution of both the ethanol solution of proline, the hydrochloric acid solution of 6-BA and gibberellin and glycine betaine is stirred mixed
Auxiliary material solution is made in conjunction；
(2) aqueous solution of potassium chloride, urea, sucrose three are added in auxiliary material solution, just miscible fluid is made in stirring；
(3) by Cu2+Solution is added in just miscible fluid, is stirred and is adjusted pH value, Tillandsia copper ion drought resistance agent is made.
5. the preparation method of Tillandsia copper ion drought resistance agent according to claim 4, it is characterised in that: step (1) institute
Stating and being stirred middle whipping temp is 40~60 DEG C, and mixing time is 2~3h.
6. the preparation method of Tillandsia copper ion drought resistance agent according to claim 4, it is characterised in that: step (2) institute
It states in stirring, whipping temp is 45~65 DEG C, and mixing time is 2~4h.
7. the preparation method of Tillandsia copper ion drought resistance agent according to claim 4, it is characterised in that: step (3) institute
It states stirring and adjusts in pH value, whipping temp is 30~45 DEG C, and mixing time is 3~5h, and adjusting pH value is 5.7~6.5.
8. the application of Tillandsia copper ion drought resistance agent described in claim 1, it is characterised in that: application Tillandsia copper ion
Drought resistance agent is to impregnate 30~40min of Tillandsia plant.
9. application of the Tillandsia copper ion drought resistance agent in Tillandsia drought resisting according to claim 8, it is characterised in that:
Temperature is controlled when applying Tillandsia copper ion drought resistance agent at 25~30 DEG C.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138560A (en) * | 2011-01-24 | 2011-08-03 | 广东省农业科学院土壤肥料研究所 | Organic nutritional drought-resistance agent preparation and preparation method thereof |
| CN102701861A (en) * | 2012-06-21 | 2012-10-03 | 魏玉芳 | Plant nutrient liquid fertilizer |
| CN103535380A (en) * | 2013-10-28 | 2014-01-29 | 成都新朝阳作物科学有限公司 | Crop drought resistant agent as well as application thereof |
| CN105104088A (en) * | 2015-09-22 | 2015-12-02 | 江苏农林职业技术学院 | Management method for improving cold tolerance of tillandsia through copper |
| CN107540452A (en) * | 2017-09-30 | 2018-01-05 | 句容市空凤来仪商贸有限公司 | A kind of nutrient solution with bactericide suitable for Tillandsia |
-
2018
- 2018-09-29 CN CN201811144897.XA patent/CN109329305A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138560A (en) * | 2011-01-24 | 2011-08-03 | 广东省农业科学院土壤肥料研究所 | Organic nutritional drought-resistance agent preparation and preparation method thereof |
| CN102701861A (en) * | 2012-06-21 | 2012-10-03 | 魏玉芳 | Plant nutrient liquid fertilizer |
| CN103535380A (en) * | 2013-10-28 | 2014-01-29 | 成都新朝阳作物科学有限公司 | Crop drought resistant agent as well as application thereof |
| CN105104088A (en) * | 2015-09-22 | 2015-12-02 | 江苏农林职业技术学院 | Management method for improving cold tolerance of tillandsia through copper |
| CN107540452A (en) * | 2017-09-30 | 2018-01-05 | 句容市空凤来仪商贸有限公司 | A kind of nutrient solution with bactericide suitable for Tillandsia |
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Application publication date: 20190215 |