CN109329301A - Tillandsia drought resistance agent and its preparation method and application - Google Patents

Tillandsia drought resistance agent and its preparation method and application Download PDF

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Publication number
CN109329301A
CN109329301A CN201811145122.4A CN201811145122A CN109329301A CN 109329301 A CN109329301 A CN 109329301A CN 201811145122 A CN201811145122 A CN 201811145122A CN 109329301 A CN109329301 A CN 109329301A
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tillandsia
drought resistance
resistance agent
solution
preparation
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丁久玲
郑凯
丁文博
王敏
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Jiangsu Polytechnic College of Agriculture and Forestry
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Jiangsu Polytechnic College of Agriculture and Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06Aluminium; Calcium; Magnesium; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action

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Abstract

The present invention provides a kind of Tillandsia drought resistance agent and its preparation method and application, and Tillandsia drought resistance agent of the invention includes the raw material components of following concentration: the SiO of mass percent concentration 0.2~0.4%3 2‑The MgSO of solution and mass percent concentration 12~25%4Solution.Tillandsia drought resistance agent provided by the invention has good drought resisting effect to Tillandsia, and the wilting coefficient of Tillandsia is reduced by 74.0% relative to blank control maximum;Relative conductivity reduces by 69.3%, and water content increases 75.8%;Meanwhile the preparation method of the drought resistance agent is simple and effective, is not required to add any industrial chemicals, it is without causing harm to be applied to Tillandsia.

Description

Tillandsia drought resistance agent and its preparation method and application
Technical field
The present invention relates to drought resistance agent with and the preparation method and application thereof more particularly to a kind of Tillandsia drought resistance agent and its system Preparation Method and application.
Background technique
Tillandsia (scientific name Tillandsia, English name Air plant) is pineapple family (Bromeliaceae) Tillandsia (Tillandsia) herbaceos perennial, including nearly 550 kinds and 90 mutation, it is various in style, come in every shape.China is Just start to introduce this plant in recent years.Because (in air, when cultivation is not required to mud to the exclusive feature of Tillandsia plant for growth Soil, and have the characteristics that absorb the harmful substances such as formaldehyde, heavy metal), just liked and paid close attention to by many people once introducing.
Currently, Tillandsia is studied in China is still in the elementary step, cultivation technique owes maturation.Ornamental plant is come It says, water management is crucial.Modern society's most people operating pressure is big, busy, has no time to attend to the plant of indoor plantation, old friend Wish that the plant of indoor plantation has certain ornamental value again under conditions of negligence of management, that is, wish that the plant of plantation is resistance to Arid.
Summary of the invention
Goal of the invention: the first object of the present invention is to provide a kind of Tillandsia survival rate and growth quality of effectively improving Drought resistance agent;The second object of the present invention is to provide the preparation method of the Tillandsia drought resistance agent;The third object of the present invention is to mention For the application of the Tillandsia drought resistance agent.
Technical solution: the present invention provides a kind of Tillandsia drought resistance agent, the raw material components including following concentration: quality percentage The SiO of specific concentration 0.2~0.4%3 2-The MgSO of solution and mass percent concentration 12~25%4Solution.
Preferably, SiO3 2-Solution is the pure Na of chemistry2SiO3The aqueous solution or hydrochloric acid solution being formulated.
Above-mentioned Tillandsia drought resistance agent further includes the raw material components of following concentration: the 6-BA solution of 10~15mg/L, 100 The potassium dihydrogen phosphate of~180mg/L, the fructose soln of 6~10g/L, the fulvic acid of 50~100mg/L, 10~18mg/L The gibberellin of malic acid and 8~15mg/L.
Wherein, 6-BA is 6- benzyl aminoadenine, alias: 6-benzyl aminopurine, the basic element of cell division, English common name: 6- Benzylaminopurine, molecular formula: C12H11N5
The preparation method of above-mentioned Tillandsia drought resistance agent, includes the following steps:
(1) it is mixed the aqueous solution of three kinds of malic acid, fulvic acid and gibberellin substances to obtain mixed solution A, by 6- The hydrochloric acid solution of BA is added mixed solution A and forms just miscible fluid;
(2) by SiO3 2-、MgSO4, four kinds of substances of potassium dihydrogen phosphate and fructose aqueous solution be mixed to obtain mixed solution Mixed solution B is added in above-mentioned primary solution by B, stirs and adjust pH value, obtains the Tillandsia drought resistance agent.
Preferably, in step (1), the whipping temp is 30~35 DEG C, and mixing time is 1~2h.
Further, in step (1), just miscible fluid is that stirring forms just miscible fluid for the formation.
Preferably, above-mentioned whipping temp is 35~45 DEG C, and mixing time is 2~3h.
Preferably, the hydrochloric acid solution of the 6-BA is that 6-BA is dissolved in concentration to be in the hydrochloric acid solution of 1mol/L, when dissolution Temperature is 40~60 DEG C, and mixing time is 1~2h.
Further, the malic acid, three kinds of substances of fulvic acid and gibberellin aqueous solution be by malic acid, fulvic acid, Three kinds of substance difference of gibberellin are soluble in water, and solution temperature is 30~35 DEG C, and mixing time is made for 1~2h.
Further, in step (2), the mixing temperature is 40~50 DEG C, and mixing time is 1~2h.
Further, in step (2), the whipping temp is 40~60 DEG C, and mixing time is 2~4h;The adjusting acid Basicity is that adjusting pH value is 5.8~6.8.
Preferably, the SiO3 2-、MgSO4, four kinds of substances of potassium dihydrogen phosphate and fructose aqueous solution be by SiO3 2-、 MgSO4, potassium dihydrogen phosphate, fructose four kinds of substances difference it is soluble in water, its solution temperature is 30~50 when four kinds of substances dissolutions DEG C, mixing time is that 1~2h is made.
The application of above-mentioned Tillandsia drought resistance agent, in the application, apply the humidity 75 of the Tillandsia drought resistance agent~ 90%;Shading rate is 50~60%;Application temperature is at 25~30 DEG C.
Preferably, the application Tillandsia drought resistance agent is that 30~40min of Tillandsia plant is impregnated in drought resistance agent.
The utility model has the advantages that Tillandsia drought resistance agent provided by the invention, has good drought resisting effect to Tillandsia, it will be empty The wilting coefficient of gas pineapple reduces by 74.0% relative to blank control maximum;Relative conductivity reduces by 69.3%, and water content increases 75.8%;Meanwhile the preparation method of the drought resistance agent is simple and effective, is not required to add any industrial chemicals, is applied to Tillandsia not It can be damaged.
Specific embodiment
It is described further below for technical solution of the present invention.
The invention will be further described below.From Shanghai, Chinese medicines group chemical reagent has raw material of the invention with reagent Limit company buys.
Embodiment 1
The preparation of Tillandsia drought resistance agent
6-BA is dissolved in the hydrochloric acid solution that concentration is 1mol/L, temperature is 40 DEG C when dissolution, mixing time 1h;By apple Tartaric acid, fulvic acid, gibberellin three kinds of substances difference are soluble in water, and solution temperature is 30 DEG C, mixing time 1h, and by three kinds of water Solution is mixed, and the temperature that three kinds of solution is mixed is 30 DEG C, time 1h;The hydrochloric acid solution of 6-BA is added to Malic acid, fulvic acid, gibberellin mixed liquor in, be mixing uniformly to form just miscible fluid, whipping temp be 35 DEG C, mixing time For 2h.
By SiO3 2-、MgSO4, potassium dihydrogen phosphate, fructose four kinds of substances difference it is soluble in water, its is molten when four kinds of substances dissolutions Solving temperature is 30 DEG C, and mixing time is 1h, and four kinds of aqueous solutions are mixed later, and the temperature of mixing is 40 DEG C, mixing time 1h, mixed solution after mixing evenly is added in primary solution, secondary stirring is carried out, secondary stirring Temperature is 40 DEG C, mixing time 2h, and stirring should sufficiently, and the concentration of each substance is respectively SiO3 2-Mass percent concentration 0.2%, MgSO4Mass percent 12%, 6-BA concentration 10mg/L, biphosphate potassium concn 100mg/L, fructose concentration 6g/L, Fulvic acid concentration 50mg/L, malic acid concentration 10mg/L, gibberellin concentration 8mg/L.The pH value of solution is adjusted to 5.8.
Tillandsia plant 30min is impregnated using Tillandsia drought resistance agent, is later taken out plant, being placed in environment temperature is 25 DEG C, conventional cultivating and managing during which is carried out to Tillandsia.The result shows that drought resisting effect is good.
Embodiment 2
The preparation of Tillandsia drought resistance agent
6-BA is dissolved in the hydrochloric acid solution that concentration is 1mol/L, temperature is 60 DEG C when dissolution, mixing time 2h;By apple Tartaric acid, fulvic acid, gibberellin three kinds of substances difference are soluble in water, and solution temperature is 35 DEG C, and mixing time is 2h, and by three Kind aqueous solution is mixed, and the temperature that three kinds of solution is mixed is 35 DEG C, time 2h;The hydrochloric acid solution of 6-BA is added Enter to malic acid, fulvic acid, gibberellin mixed liquor in, be mixing uniformly to form just miscible fluid, whipping temp be 45 DEG C, stirring Time is 3h.
By SiO3 2-、MgSO4, potassium dihydrogen phosphate, fructose four kinds of substances difference it is soluble in water, its is molten when four kinds of substances dissolutions Solving temperature is 50 DEG C, and mixing time is 2h, and four kinds of aqueous solutions are mixed later, and the temperature of mixing is 50 DEG C, mixing time 2h, mixed solution after mixing evenly is added in primary solution, secondary stirring is carried out, secondary stirring Temperature is 60 DEG C, mixing time 4h, and stirring should sufficiently, and the concentration of each substance is respectively SiO3 2-Mass percent concentration 0.4%, MgSO4Mass percent 25%, 6-BA concentration 15mg/L, biphosphate potassium concn 180mg/L, fructose concentration 10g/L, Fulvic acid concentration 100mg/L, malic acid concentration 18mg/L, gibberellin concentration 15mg/L.The pH value of solution is adjusted to 6.8.
Tillandsia plant 40min is impregnated using Tillandsia drought resistance agent, is later taken out plant, being placed in environment temperature is 30 DEG C, conventional cultivating and managing during which is carried out to Tillandsia.The result shows that drought resisting effect is good.
Embodiment 3
The preparation of Tillandsia drought resistance agent
6-BA is dissolved in the hydrochloric acid solution that concentration is 1mol/L, temperature is 50 DEG C when dissolution, mixing time 1.5h;It will Malic acid, fulvic acid, gibberellin three kinds of substances difference are soluble in water, and solution temperature is 33 DEG C, mixing time 1.5h, and by three Kind aqueous solution is mixed, and the temperature that three kinds of solution is mixed is 33 DEG C, time 1.5h;By the hydrochloric acid solution of 6-BA It is added in the mixed liquor of malic acid, fulvic acid, gibberellin, is mixing uniformly to form just miscible fluid, whipping temp is 40 DEG C, is stirred Mixing the time is 2.5h.
By SiO3 2-、MgSO4, potassium dihydrogen phosphate, fructose four kinds of substances difference it is soluble in water, its is molten when four kinds of substances dissolutions Solving temperature is 40 DEG C, and mixing time is 1.5h, and four kinds of aqueous solutions are mixed later, and the temperature of mixing is 45 DEG C, mixing time 1.5h, mixed solution after mixing evenly is added in primary solution, carries out secondary stirring, and secondary is stirred The temperature mixed is 50 DEG C, mixing time 3h, and stirring should sufficiently, and the concentration of each substance is respectively SiO3 2-Mass percent it is dense Spend 0.3%, MgSO4Mass percent 20%, 6-BA concentration 13mg/L, biphosphate potassium concn 150mg/L, fructose concentration 8g/ L, fulvic acid concentration 80mg/L, malic acid concentration 15mg/L, gibberellin concentration 10mg/L.The pH value of solution is adjusted to 6.0.
Tillandsia plant 35min is impregnated using Tillandsia drought resistance agent, is later taken out plant, being placed in environment temperature is 28 DEG C, conventional cultivating and managing during which is carried out to Tillandsia.The result shows that drought resisting effect is good.
Embodiment 4
The concentration of Tillandsia drought resistance agent primary raw material component selects
(1) experimental group model construction
Each Tillandsia kind selects the almost the same plant of size, the short leaf of dead leaf around plant is wiped out, in greenhouse Middle recovery carries out drought stress after half a month.Temperature control is set in the greenhouse, summer temp control hides in 28 DEG C, blower, sunshade net, summer Light rate be 50%-60%, cascade, it is micro- spray etc. equipment, air humidity 75%-90%, daily divulge information.Drought stress: by air phoenix Pears plant is placed in outdoor outdoor place and carries out drought stress processing, herein without shade, full exposure;The method of drought stress processing is to connect The continuous 15d that do not water, does not have rainwater during drought stress.Daily morning 8:00-8:30, noon 14:00- during drought stress 14:30 and 17:00-17:30 in afternoon measurement outdoor temperature and intensity of illumination, outdoor day samming during counting drought stress Are as follows: 26.7 DEG C, daytime per day intensity of illumination are as follows: 65264lux.
Tillandsia plant is placed in greenhouse (greenhouse experiment is same as above) water spray and moistened completely to plant after drought stress and is It is quasi-.Tillandsia drought resistance agent, SiO are prepared with raw material components according to the step of embodiment 3 within second day3 2-Solution is set as 7 concentration, With SiO3 2-Weight ratio meter, percent concentration is respectively 0,0.15%, 0.20%, 0.25%, 0.30%, 0.40%, 0.45%;MgSO4Solution is set as 6 concentration, and mass percent concentration is respectively 0,10%, 12%, 20%, 25%, 28%. Two kinds of solution mixing match processing such as table 1, sets 25 processing, wherein processing 1 is control, as water altogether.Each processing sets 3 weights Multiple, each each kind of repetition has consistent 30 plants of Tillandsia plant of growth.
1 SiO of table3 2-Solution and MgSO4Solution mixing match handles table
New serial number SiO3 2-Solution concentration MgSO4Solution concentration
1 0 0
2 0.15% 12%
3 0.15% 25%
4 0.30% 10%
5 0.30% 12%
6 0.30% 20%
7 0.30% 25%
8 0.30% 28%
9 0.25% 10%
10 0.25% 12%
11 0.25% 20%
12 0.25% 25%
13 0.25% 28%
14 0.40% 10%
15 0.40% 12%
16 0.40% 20%
17 0.40% 25%
18 0.40% 28%
19 0.20% 10%
20 0.20% 12%
21 0.20% 20%
22 0.20% 25%
23 0.20% 28%
24 0.45% 12%
25 0.45% 25%
The solution of each processing respective concentration impregnates Tillandsia plant 0.5h, later takes out plant, is placed in greenhouse. Routine Management is carried out to Tillandsia during test.Tillandsia adds magnesium mixed liquor treated that third day starts daily by silicon Water spray is primary, and water spray standard is subject to plant is completely wet, water spraying time 8:00-9:00 or 4:00-6:00 in afternoon in the morning;Often It is primary to spray the Tillandsia special fertilizer that N P and K proportion is 20:10:20 week, spraying concentration is 1000-1500 times of dilution.
(2) measurement item
The upgrowth situation that each processing Tillandsia is observed in treatment process, records its wilting plant number, calculates wilting coefficient. Wilting coefficient=processing of death plant number * 100/ plant number.
Processing measures the conductivity of each processing Tillandsia after 1 month.Specific determination step is as follows: respectively handling each repetition 5 plants are randomly selected, the acquisition leaf of falling 2-3 (counts 2-3 leaf from lobus cardiacus) outward, measures turbine blades in electrochemical matter leaching rate with conductivity gauge method.Phase Rear conductivity is boiled to conductivity (%)=original conductivity * 100/.
Processing measures the leaf r elative water content of each processing Tillandsia after 1 month.It is measured and is counted using weighting method after dried Calculate leaf r elative water content.Leaf r elative water content=(Wf-Wd) * 100/ (Wt-Wd), Wf is fresh weight in formula, and Wd is leaf Piece dry weight, Wt are that blade is saturated fresh weight.
(3) interpretation of result
Influence of the 3.1 Tillandsia drought resistance agents processing to Tillandsia wilting coefficient after drought stress
Test plant material is handled after drought stress with the Tillandsia drought resistance agent of various concentration, 1 month its Wilting coefficient is different, is shown in Table 2.
The wilting coefficient of 2 Tillandsia of table (wilting coefficient unit is %)
This research is reflected by wilting coefficient applies Tillandsia drought resistance agent to Tillandsia plant under drought stress Situation is survived, wilting coefficient is lower, and plant survival rate is lower;Conversely, Tillandsia survival rate is higher.As shown in Table 2, empty for examination In gas pineapple kind, its wilting coefficient compared with control group processing 1 of processing 2,3,4,8,9,13,14,18,19,23,24,25 Difference is not significant, and wilting coefficient is higher.The wilting coefficient of remaining processing group is lower, significantly lower than control and its elsewhere Reason.Thus can illustrate, the Tillandsia drought resistance agent for applying certain silicon magnesium density outside can reduce withering for Tillandsia after drought stress Listless coefficient.Only when silicon magnesium density is suitable for, plant wilt coefficient can be just significantly reduced.This research is thought, when 0.2~0.4% SiO3 2-Solution and 12~25% MgSO4The wilting of Tillandsia after drought stress can be significantly reduced when solution mixed processing Coefficient, significantly improves the drought resistance of Tillandsia, to promote the growth of Tillandsia plant.
Influence of the 3.2 Tillandsia drought resistance agents processing to Tillandsia relative conductivity after drought stress
The relative conductivity of 3 Tillandsia plant of table (relative conductivity unit is %)
With the relative conductivity of electrical conductivity method measurement plant leaf blade, relative conductivity can reflect the strong of the drought resistance of plant It is weak.When drought stress occurs, blade cell film is easy to be damaged, and membrane permeability increases, and cell inclusion extravasation leads to outer sepage Conductivity increases, and injury of plasmalemmae degree increases, and drought resistance weakens.Relative conductivity is higher, and the drought resistance of plant is lower;It is relatively electric Conductance is lower, illustrates that the drought resistance of plant is higher.This research will carry out Tillandsia by the Tillandsia kind of drought stress Drought resistance agent processing determines whether silicon magnesium can improve Tillandsia by the relative conductivity of each kind under measurement different disposal The influence of drought resistance and its concentration to drought resisting effect.
As shown in Table 3, for examination Tillandsia kind in, processing 2,3,4,8,9,13,14,18,19,23,24,25 with compare Group processing 1 compares, and its relative conductivity difference is not significant, and relative conductivity is higher.Remaining processing relative conductivity compared with It is low, significantly lower than control and other processing.Thus can illustrate, the Tillandsia drought resistance agent for applying certain silicon magnesium density outside can drop The relative conductivity of Tillandsia after low drought stress.Only when silicon magnesium density is suitable for, it is relatively electric can just to significantly reduce plant Conductance.As 0.2~0.4% SiO3 2-Solution and 12~25% MgSO4Drought stress can be significantly reduced when solution mixed processing The relative conductivity of Tillandsia afterwards, significantly improves the drought resistance of Tillandsia, to promote the growth of Tillandsia plant.
Influence of the 3.3 Tillandsia drought resistance agents processing to Tillandsia leaf r elative water content after drought stress
4 Tillandsia leaf r elative water content of table (leaf r elative water content unit is %)
New serial number Dimension road orange red is received Elfin Si Chuikete Bake is vertical
1 39.8 40.7 40.5 38.6
2 42.5 42.3 42.7 43.6
3 45.0 44.3 41.8 43.5
4 45.2 45.0 41.3 42.4
5 68.8 69.5 68.2 69.4
6 70.2 70.6 68.6 67.4
7 67.5 69.4 70.4 68.7
8 44.0 44.5 43.7 45.0
9 43.7 42.6 43.6 45.8
10 70.2 69.5 67.4 67.3
11 70.4 67.6 69.5 66.5
12 67.4 71.1 67.8 68.2
13 43.1 42.7 44.2 45.5
14 45.6 44.2 42.5 41.2
15 62.9 67.3 65.2 70.5
16 66.4 64.5 65.0 69.8
17 68.9 71.0 71.2 68.5
18 43.2 44.3 43.7 42.6
19 46.0 43.2 46.6 43.8
20 68.5 69.2 70.4 71.3
21 70.5 70.2 68.8 67.5
22 69.9 66.8 67.4 70.4
23 45.0 45.7 46.2 46.3
24 43.7 43.2 44.0 42.7
25 40.5 45.5 44.2 43.6
Moisture is the basis for maintaining the effect of plant normal physiological, and leaf r elative water content can reflect plant internal water Wane degree, and Under Drought Stress in Plant leaf r elative water content is higher, and water retention capability is stronger, and plant drought resistance is stronger.
The leaf r elative water content of Tillandsia is shown in Table 4 after the processing of Tillandsia drought resistance agent.As shown in Table 4, for trying air In pineapple kind, the leaf r elative water content of each Tillandsia drought resistance agent processing is above control, handle 2,3,4,8,9,13, 14,18,19,23,24,25 compared with control group processing 1, a little higher than control of leaf r elative water content, but compared with the control Difference is not significant;The leaf r elative water content of remaining processing is significantly higher than and compares.Thus it can illustrate, it is dense to apply certain silicon magnesium outside The leaf r elative water content of Tillandsia after drought stress can be improved in the Tillandsia drought resistance agent of degree.It is only suitable in silicon magnesium density When suitable, it is just remarkably improved plant leaf blade relative water content.As 0.2~0.4% SiO3 2-Solution and 12~25% MgSO4 It is remarkably improved the leaf r elative water content of Tillandsia after drought stress when solution mixed processing, significantly improves Tillandsia Drought resistance, to promote the growth of Tillandsia plant.
It is concluded that three comprehensive wilting coefficient, relative conductivity and leaf r elative water content indexs, drought stress Afterwards, the drought resistance of Tillandsia can be improved in certain density silicon magnesium in Tillandsia drought resistance agent, as 0.2~0.4% SiO3 2- Solution and 12~25% MgSO4The wilting coefficient and relative conductivity of Tillandsia can be significantly reduced when solution mixed processing, Its leaf water content is dramatically increased, to significantly improve Tillandsia drought resistance.
Comparative example 1
Influence of the 6-BA to Tillandsia copper ion drought resistance agent effect
It is added without 6-BA, remaining prepares Tillandsia copper ion drought resistance agent according to the method and raw material components of embodiment 3, According to the method for embodiment 4, measure that the vertical wilting coefficient in Tillandsia kind Bake is 37.8%, relative conductivity is 60.7%, relative water content 40.9%.Difference is not significant compared with the control for each testing index, illustrates to be added without 6-BA not The vertical drought resistance in Tillandsia kind Bake can significantly be improved.
Comparative example 2
Influence of the potassium dihydrogen phosphate to Tillandsia copper ion drought resistance agent effect
It is added without potassium dihydrogen phosphate, remaining prepares Tillandsia copper ion according to the method and raw material components of embodiment 3 Drought resistance agent, according to the method for embodiment 4, measuring the vertical wilting coefficient in Tillandsia kind Bake is 40.3%, relative conductivity For 59.7%, relative water content 39.6%.Difference is not significant compared with the control for each testing index, illustrates to be added without phosphoric acid Potassium dihydrogen cannot significantly improve the vertical drought resistance in Tillandsia kind Bake.
Comparative example 3
Influence of the fructose to Tillandsia copper ion drought resistance agent effect
It is added without fructose, remaining prepares Tillandsia copper ion drought resistance agent according to the method and raw material components of embodiment 3, According to the method for embodiment 4, measure that the vertical wilting coefficient in Tillandsia kind Bake is 42.1%, relative conductivity is 59.8%, relative water content 40.3%.Difference is not significant compared with the control for each testing index, illustrates to be added without fructose not The vertical drought resistance in Tillandsia kind Bake can significantly be improved.
Comparative example 4
Influence of the fulvic acid to Tillandsia copper ion drought resistance agent effect
It is added without fulvic acid, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3 Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 39.7%, relative conductivity is according to the method for embodiment 4 62.5%, relative water content 43.6%.Difference is not significant compared with the control for each testing index, illustrates to be added without fulvic acid The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.
Comparative example 5
Influence of the gibberellin to Tillandsia copper ion drought resistance agent effect
It is added without gibberellin, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3 Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 42.2%, relative conductivity is according to the method for embodiment 4 59.5%, relative water content 41.4%.Difference is not significant compared with the control for each testing index, illustrates to be added without gibberellin The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.
Comparative example 6
Influence of the malic acid to Tillandsia copper ion drought resistance agent effect
It is added without malic acid, remaining prepares the drought resisting of Tillandsia copper ion according to the method and raw material components of embodiment 3 Agent measures that the vertical wilting coefficient in Tillandsia kind Bake is 42.8%, relative conductivity is according to the method for embodiment 4 60.5%, relative water content 40.5%.Difference is not significant compared with the control for each testing index, illustrates to be added without malic acid The vertical drought resistance in Tillandsia kind Bake cannot significantly be improved.

Claims (10)

1. a kind of Tillandsia drought resistance agent, which is characterized in that the raw material components including following concentration: mass percent concentration 0.2 ~0.4% SiO3 2-The MgSO of solution and mass percent concentration 12~25%4Solution.
2. a kind of Tillandsia drought resistance agent according to claim 1, which is characterized in that further include the raw material group of following concentration Point: the 6-BA solution of 10~15mg/L, the potassium dihydrogen phosphate of 100~180mg/L, the fructose soln of 6~10g/L, 50~ The gibberellin of the fulvic acid of 100mg/L, the malic acid of 10~18mg/L and 8~15mg/L.
3. the preparation method of Tillandsia drought resistance agent described in claim 2, which comprises the steps of:
(1) it is mixed the aqueous solution of three kinds of malic acid, fulvic acid and gibberellin substances to obtain mixed solution A, by 6-BA's Hydrochloric acid solution is added mixed solution A and forms just miscible fluid;
(2) by SiO3 2-、MgSO4, four kinds of substances of potassium dihydrogen phosphate and fructose aqueous solution be mixed to obtain mixed solution B, will Mixed solution B is added in above-mentioned primary solution, stirs and adjust pH value, obtains the Tillandsia drought resistance agent.
4. a kind of preparation method of Tillandsia drought resistance agent according to claim 3, it is characterised in that: described in step (1) Whipping temp is 30~35 DEG C, and mixing time is 1~2h.
5. a kind of preparation method of Tillandsia drought resistance agent according to claim 3, it is characterised in that: described in step (1) Forming just miscible fluid is that stirring forms just miscible fluid.
6. a kind of preparation method of Tillandsia drought resistance agent according to claim 5, it is characterised in that: the whipping temp is 35~45 DEG C, mixing time is 2~3h.
7. a kind of preparation method of Tillandsia drought resistance agent according to claim 3, it is characterised in that: described in step (2) It is 40~50 DEG C that temperature, which is mixed, and mixing time is 1~2h.
8. a kind of preparation method of Tillandsia drought resistance agent according to claim 3, it is characterised in that: described in step (2) Whipping temp is 40~60 DEG C, and mixing time is 2~4h;The adjusting pH value is that adjusting pH value is 5.8~6.8.
9. the application of Tillandsia drought resistance agent as claimed in claim 1 or 2, it is characterised in that: in the application, application temperature is 25 ~30 DEG C.
10. the application of Tillandsia drought resistance agent according to claim 9, it is characterised in that: applying Tillandsia drought resistance agent is 30~40min of Tillandsia plant is impregnated in drought resistance agent.
CN201811145122.4A 2018-09-29 2018-09-29 Tillandsia drought resistance agent and its preparation method and application Pending CN109329301A (en)

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