CN103477886A - Method for testing influence of different 6-BA concentration on development of air plant tiller bud - Google Patents
Method for testing influence of different 6-BA concentration on development of air plant tiller bud Download PDFInfo
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Abstract
The invention discloses a method for testing the influence of different 6-BA concentration on development of air plant tiller buds. By setting different 6-BA concentration to treat seven air plant varieties, the result that 6-BA can obviously promote the development of the air plant tiller buds can be obtained, the reproduction speed of air plants is accelerated and different 6-BA concentration is suitable for different varieties, wherein the quantity of tiller buds of T. fuego and T. harrisii is the largest when the 6-BA concentration is 40-100mg/L, the quantity of tiller buds of T. ionantha rubra and T ionantha ionantha is the largest when the 6-BA concentration is 60-100mg/L, the quantity of tiller buds of T. tricolor is the largest when the 6-BA concentration is 60-120mg/L, the quantity of tiller buds of T. velutina is the largest when the 6-BA concentration is 100-160mg/L and the quantity of tiller buds of T. brachycaulos is the largest when the 6-BA concentration is 140-180mg/L.
Description
Technical field
The invention belongs to the cultivation of plants technical field, be specifically related to the test method of a kind of different 6-BA concentration on the impact of Tillandsia tiller bud development.
Background technology
Tillandsia (formal name used at school Tillandsia, English name Air plant) be pineapple family (Bromeliaceae) Tillandsia (Tillandsia) perennial herb, its morphological feature and growth pattern are different from common plant, be grown in air, without earth, without flowerpot, can appreciate leaf, considerable flower, have the characteristics such as good decorating effect, strong adaptability again; There is the special role purified the air of a room simultaneously.Before more than 100 years, just someone cultivates and has viewed and admired Tillandsia, be widely current the eighties in 20th century abroad, abroad cultivation and the industrialization development of Tillandsia are paid much attention to, as all there are professional administrative staff in the countries such as the U.S., Guatemala and take up an area the large intelligent greenhouse of nearly thousand mu, specialize in exploitation and the sale of Tillandsia research, production and serial ornaments thereof, handicraft.Abroad about the research of Tillandsia, focus mostly on as aspects such as air pollution indicator plant, distributed areas, metabolic waies.Tillandsia is very rare in China, the provenance rareness, and the current research to Tillandsia is in the elementary step, and domestic only have the research of a small amount of some summaries about Tillandsia, tissue cultivation and radiation proof aspect to report.Be necessary the breeding of Tillandsia is studied, accelerate its reproduction speed, enlarge the germ plasm resource of China's air.
Main seminal propagation and the division propagation of relying on of Tillandsia breeding, the reproduction speed of seminal propagation is slow, the normal division propagation that adopts, but under nature, Tillandsia can produce tiller bud from axil or base portion after blooming, and the situation of tillering and its florescence are closely related, the sub-strain of only just tillering after the florescence, and limited amount, 1-2 the sub-strain of can tillering at most of maternal plant.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide that a kind of test method is simple, easy to operate, the reliable different 6-BA concentration of data is on the test method of the impact of Tillandsia tiller bud development.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
The test method of a kind of different 6-BA concentration on the impact of Tillandsia tiller bud development, the method comprises the steps:
1) at first prepare the 6-BA solution of 11 kinds of variable concentrations; The 6-BA solution prepared is labelled, standby respectively;
2) 3 repetitions are established in each each processing of Tillandsia kind, and each repeats to choose 30 strain plants of uniform size, and withered, dead leaf are wiped out, and are placed in greenhouse to recover growth two Zhou Houzai and processed;
3) the 6-BA solution that test plant is placed in respectively to the variable concentrations described in step 1) soaks;
4) drain away the water, be placed in greenhouse, and carry out the management of normal waste water, damage by disease and insect;
5) check the tiller number of each kind of Tillandsia after 3 months.
In step 1), the concentration of the 6-BA solution of variable concentrations is respectively: 0mg/L, 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 140mg/L, 160mg/L, 180mg/L, 200mg/L, the 6-BA solution that wherein concentration is 0mg/L is control group, and remaining concentration is processed group.The concentration arranged is established a concentration with the form of arithmetic progression every 20mg/L and is processed, and has established altogether 11 concentration and has processed, and the gradient of setting is wide, the 6-BA concentration of tillering fast to filtering out each kind of suitable Tillandsia the concentration gradient from as much as possible.
Step 2) the Tillandsia kind described in comprises that Fei Ge, Lu Bula, Ying Te, three look iron orchids, Harris, Wei Lutina, Bake are vertical.China's Tillandsia resource is relatively less, and 7 Tillandsia kinds that this test is chosen are domestic comparatively common kinds, can be used for carrying out Fast-propagation.
The condition in described greenhouse is: air humidity is 75%～85%; Temperature is 20 ℃-30 ℃; Shading rate is 50%-75%.According to managerial experiences for many years, be beneficial to the growth of Tillandsia under this greenhouse experiment.
Beneficial effect: compared with prior art, advantage of the present invention is: test method is simple, easy to operate, data are reliable, 6-BA can obviously promote the growth of Tillandsia tiller bud, accelerate the reproduction speed of Tillandsia, the 6-BA concentration difference that different kinds is suitable, Fei Ge and Harris are that 40-100mg/L tiller bud quantity is maximum in 6-BA concentration, Lu Bula and Ying Te are maximum at 60-100mg/L, three look iron orchids are maximum at 60-120mg/L, Wei Lu carries that to be contained in 100-160mg/L maximum, and it is maximum that Bake stands in 140-180mg/L.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete content of embodiment is only for the present invention is described, and should also can not limit the present invention described in detail in claims.
The test method of a kind of different 6-BA concentration of the present invention on the impact of Tillandsia tiller bud development, the method comprises the steps:
1) at first prepare the 6-BA solution of 11 kinds of variable concentrations; The 6-BA solution prepared is labelled, standby respectively; The concentration of the 6-BA solution of variable concentrations is respectively: 0mg/L, 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 140mg/L, 160mg/L, 180mg/L, 200mg/L, each concentration contrasts with CK(respectively), T
1, T
2, T
3, T
4, T
5, T
6, T
7, T
8, T
9, T
10mean.The 6-BA solution that wherein concentration is 0mg/L is control group, T
1, T
2, T
3, T
4, T
5, T
6, T
7, T
8, T
9, T
10be processed group;
2) selecting the test plant material is luxuriant and rich with fragrance dagger-axe (T.fuego), Ying Te (T.ionantha ionantha), Lu Bula (T.ionantha rubra), Bake vertical (T.brachycaulos), Harris (T.harrisii), three look iron blue (T.tricolor) and 7 Tillandsia kinds of Wei Lutina (T.velutina), and plant is individual plant and does not bloom;
3 repetitions are established in each each processing of Tillandsia kind, and each repeats to choose 30 strain plants of uniform size, and withered, dead leaf are wiped out, and are placed in greenhouse to recover growth two Zhou Houzai and processed;
3) the 6-BA solution that test plant is placed in respectively to the variable concentrations described in step 1) soaks 30min;
4) drain away the water, be placed in greenhouse, and carry out the management of normal waste water, damage by disease and insect;
5) check the tiller number of each kind of Tillandsia after 3 months.
The condition of said method medium temperature chamber is: air humidity is 75%～85%; Temperature is 20 ℃-30 ℃; Shading rate is 50%-75%.
Result by above-mentioned 7 Tillandsia kinds after variable concentrations is processed is carried out data processing and statistical analysis with Excel and SAS ver8.0 software.
6-BA processes the tiller bud quantity difference of lower each kind, with the individual plant tiller number, means, in Table 1; Show compared with the control otherness in various degree, embody with multiple, in Table 2.In 7 Tillandsia kinds, except luxuriant and rich with fragrance dagger-axe, the individual plant tiller number of all the other kinds under 6-BA processes all is significantly higher than contrast.When Fei Ge is 40-100mg/L in 6-BA concentration, tiller number is more, reaches 7.21-8.54/strain, is significantly higher than other concentration and processes, and is 3.28-3.82 times of contrast.6-BA concentration is not more high more is beneficial to tillering of luxuriant and rich with fragrance dagger-axe, and when concentration, during higher than >=140mg/L, its tiller number is lower than contrast, and the situation of plant death even appears in severe patient.T wherein
2(40mg/L) the lower individual plant tiller number of processing is maximum.
It is more that tiller number is foretold when being pulled in 6-BA concentration and being 60-100mg/L in Shandong, is 5.59-7.12/strain, is significantly higher than other most of concentration and processes, T
1except, be the 4.21-5.18 of contrast tiller number doubly.T wherein
4(80mg/L) the lower individual plant tiller number of processing is the highest.
When Ying Te is 60-100mg/L in 6-BA concentration, tiller number is more, is 4.40-5.78/strain, is significantly higher than other and processes, and is 4.38-5.50 times of contrast.T wherein
3(60mg/L) the lower individual plant tiller number of processing is maximum.
Three look iron orchids tiller number when 6-BA concentration is 60-120mg/L is more, is 6.41-10.56/strain, is significantly higher than other and processes, and is 7.41-11.56 times of contrast.T wherein
4(80mg/L) the lower individual plant tiller number of processing is maximum.
When Harris is 40-100mg/L in 6-BA concentration, tiller number is more, is 1.48-1.90/strain, is 2.36-2.90 times of contrast, and these four processing are except T
4outer its excess-three processing all is significantly higher than other processing.T wherein
3(60mg/L) the lower individual plant tiller number of processing is maximum.
Wei Lu carries being contained in tillers morely when 6-BA concentration is 100-160mg/L, be 3.07-4.72/strain, is significantly higher than other and processes, be the 2.91-4.08 of contrast doubly.T wherein
5(100mg/L) the lower individual plant tiller number of processing is maximum.
Bake stands in tillers morely when 6-BA concentration is 140-180mg/L, be 3.79-4.37/strain, is significantly higher than other and processes, be the 4.79-5.37 of contrast doubly.T wherein
8(160mg/L) the lower individual plant tiller number of processing is maximum.
6-BA processes 7 Tillandsia kind situations of tillering of participating in the experiment has been produced to impact in various degree.Wherein, Harris is subject to the minimum that affects of 6-BA, and after processing, the individual plant tiller number mostly is 1.90 most, and other 6 kinds are subject to the impact of 6-BA relatively large, and tiller number can reach 3-10.
Table 16-BA processes the individual plant tiller number of lower each kind
Annotate: indicate identical capitalization person in same row and mean difference not remarkable (p<0.05).
Table 26-BA processes lower each kind tiller number multiple compared with the control
The conclusion drawn by the inventive method is as follows:
6-BA can obviously promote Tillandsia to tiller, and has verified that the outer CTKs that executes can increase the research conclusion that the plant lateral bud occurs.Execute 6-BA outward and promote Tillandsia to tiller substantially not to be subject to florescence and season limit, satisfactory for result.
The suitable 6-BA concentration difference of different Tillandsia kinds is found in research.Fei Ge and Harris are tillered at most under 6-BA concentration 40-100mg/L condition, Lu Bula and Ying Te are tillered at most at 60-100mg/L, three look iron orchids are carried and are contained in 100-160mg/L and tiller at most on 60-120mg/L maximum ，Wei road of tillering, and Bake stands in 140-180mg/L and tillers at most.After using 6-BA, Tillandsia tiller bud quantity significantly increases, and is contrast 2 times of reproduction coefficient, or even tens times.Therefore, can use the 6-BA of debita spissitudo for promoting tillering of Tillandsia plant, but the height of concentration should be treated according to different kinds with a certain discrimination.
Although 6-BA can stimulate tiller bud to sprout, and can't maintain its further growth.In the tiller bud growth later stage, should give sufficient water and fertilizer management, particularly nutrient supply, otherwise easily cause the tiller bud growth retardation, even withered, dead.Execute 6-BA outward on the Tillandsia plant, find that there is after a large amount of tiller buds occur and should strengthen nutrition supply, thereby promote the growth of tiller bud.
Claims (4)
1. the different 6-BA concentration test method on the impact of Tillandsia tiller bud development, is characterized in that, the method comprises the steps:
1) at first prepare the 6-BA solution of 11 kinds of variable concentrations; The 6-BA solution prepared is labelled, standby respectively;
2) 3 repetitions are established in each each processing of Tillandsia kind, and each repeats to choose 30 strain plants of uniform size, and withered, dead leaf are wiped out, and are placed in greenhouse to recover growth two Zhou Houzai and processed;
3) the 6-BA solution that test plant is placed in respectively to the variable concentrations described in step 1) soaks;
4) drain away the water, be placed in greenhouse, and carry out the management of normal rich water, damage by disease and insect;
5) check the tiller number of each kind of Tillandsia after 3 months.
2. the test method of different 6-BA concentration according to claim 1 on the impact of Tillandsia tiller bud development, it is characterized in that, in step 1), the concentration of the 6-BA solution of variable concentrations is respectively: 0mg/L, 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L, 120mg/L, 140mg/L, 160mg/L, 180mg/L, 200mg/L, the 6-BA solution that wherein concentration is 0mg/L is control group, and remaining concentration is processed group.
3. the test method of different 6-BA concentration according to claim 1 on the impact of Tillandsia tiller bud development, it is characterized in that step 2) described in the Tillandsia kind comprise that Fei Ge, Lu Bula, Ying Te, three look iron orchids, Harris, Wei Lutina, Bake are vertical.
4. the test method of different 6-BA concentration according to claim 1 on the impact of Tillandsia tiller bud development, is characterized in that, the condition in described greenhouse is: air humidity is 75%～85%; Temperature is 20 ℃-30 ℃; Shading rate is 50%-75%.
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Cited By (6)
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| CN104094844A (en) * | 2014-06-26 | 2014-10-15 | 江苏农林职业技术学院 | Tissue culture and inducing culture method for Tillandsia |
| CN105075618A (en) * | 2015-08-21 | 2015-11-25 | 江苏农林职业技术学院 | Reproduction method of tillandsia usneoides |
| CN105075826A (en) * | 2015-08-21 | 2015-11-25 | 江苏农林职业技术学院 | Illumination management method used for promoting growth of air plants and combined with magnesium fertilizer applying |
| CN105104136A (en) * | 2015-08-21 | 2015-12-02 | 江苏农林职业技术学院 | Moisture and illumination managing method for promoting growth of tillandsia |
| CN109329301A (en) * | 2018-09-29 | 2019-02-15 | 江苏农林职业技术学院 | Tillandsia drought resistance agent and its preparation method and application |
| CN114467669A (en) * | 2021-12-24 | 2022-05-13 | 山西农业大学 | Method for promoting efficient tillering of ornamental pineapple |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094844A (en) * | 2014-06-26 | 2014-10-15 | 江苏农林职业技术学院 | Tissue culture and inducing culture method for Tillandsia |
| CN105075618A (en) * | 2015-08-21 | 2015-11-25 | 江苏农林职业技术学院 | Reproduction method of tillandsia usneoides |
| CN105075826A (en) * | 2015-08-21 | 2015-11-25 | 江苏农林职业技术学院 | Illumination management method used for promoting growth of air plants and combined with magnesium fertilizer applying |
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| CN105075618B (en) * | 2015-08-21 | 2017-11-07 | 江苏农林职业技术学院 | A kind of propagation method of sunglo pineapple |
| CN109329301A (en) * | 2018-09-29 | 2019-02-15 | 江苏农林职业技术学院 | Tillandsia drought resistance agent and its preparation method and application |
| CN114467669A (en) * | 2021-12-24 | 2022-05-13 | 山西农业大学 | Method for promoting efficient tillering of ornamental pineapple |
| CN114467669B (en) * | 2021-12-24 | 2023-09-22 | 山西农业大学 | Method for promoting efficient tillering of ornamental pineapple |
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