Embodiment
Embodiment 1
To crush particle diameter and be 10 purpose cream paulownia seed oil cakes and pack in the reactor, employing ethanol is extractant, presses material: the weight ratio of solvent=1: 2 adds in the material, be under 50 ℃ the condition in extraction temperature, extracted 30 minutes, Separation of Solid and Liquid makes cream paulownia seed oil cake extract behind precipitation.Respectively matrine, DCPTA, water, cream paulownia seed oil cake extract, fatty alcohol-polyoxyethylene ether are added in the reactor, the control solution temperature is 50 ℃, and the stirring and dissolving time is 20 minutes; Fulvic acid zinc is added in the reactor, and the control dispersion temperature is 50 ℃, and the dispersed with stirring time is 20 minutes; After cooling, make anti-plant virus agent.Raw materials used and the weight proportion of anti-plant virus agent of preparation is:
10 parts of cream paulownia seed oil cake extracts
10 parts of matrines
10 parts on fulvic acid zinc
5 parts of DCPTAs
10 parts of fatty alcohol-polyoxyethylene ether
20 parts in water
With the anti-plant virus agent agent of the foregoing description 1 preparation, prevent and treat the effect assessment test of the viroses of plant.
1, test objective:
Tobacco mosaic virus disease is one of important disease on the tobacco, and all there is distribution various places, causes serious economic loss to leaf tobacco production, and is therefore significant on producing to the control of tobacco mosaic virus disease.Be the control efficiency of checking anti-plant virus agent, the spy carries out the field control effectiveness test of this chemical control tobacco mosaic virus disease.
2, experimental condition:
2.1 for studying thing: K326
2.2 controlling object: common tobacco mosaic virus disease
2.3 basic condition experimental field
Test is carried out in cigarette ground, field, cities and towns, river, Jiangchuan County.
2.4 spraying time: on June 10th, 2006
3, experimental scheme and method
3.1 reagent agent
|
Chinese/formulation and content |
Production unit |
Test medicine |
The anti-plant virus agent of present embodiment 1 preparation |
|
The contrast medicament |
24% mixes resin acid alkali copper aqua |
Beijing Dongwang Pesticide Factory |
3.2 test is handled
Code name |
Test medicine |
Dispenser dosage |
Water consumption (liter/mu) |
??A |
The anti-plant virus agent of present embodiment 1 preparation |
500 times |
??50 |
??B |
The anti-plant virus agent of present embodiment 1 preparation |
1000 times |
??50 |
Code name |
Test medicine |
Dispenser dosage |
Water consumption (liter/mu) |
??C |
The anti-plant virus agent of present embodiment 1 preparation |
2000 times |
??50 |
??D |
Chemicals (poison disappears) |
500 times |
??50 |
??CK |
Clear water |
??- |
??50 |
3.3 sub-district Pareto diagram
Repeat 1 |
??A |
??B |
??C |
??D |
??CK |
Repeat 2 |
??D |
??C |
??CK |
??B |
??A |
Repeat 3 |
??B |
??CK |
??A |
??C |
??D |
4, test operation
4.1 using method: 5 processing are established in test, 3 repetitions, totally 15 sub-districts.0.5 mu of sub-district area, sub-district are arranged and are pressed table 3.3 randomized arrangement, with " bodyguard " board 16 type backpack blade face manual sprayer even spraying, and equivalent clear water spraying blank district.
4.2 the time of dispenser and number of times
4.2.1 control test: in spray in the June 10 medicine first time, spray in 7 days medicine in every interval amounts to 5 times.
4.3 investigation method
4.3.1 control test: respectively at investigation before the dispenser once, investigation in 7,14,21,28,35 days is 1 time behind the medicine, is unit with the strain, district by district by strain investigation diseased plant rate and disease index.
5, drug effect is calculated
The incidence of disease (%)=(the morbidity strain number/total strain number of investigation) * 100%
Disease index=[∑ (diseased plants at different levels * this disease level value)/(investigating total strain number * 4)] * 100%
Relative control effect %=[(contrast disease index one is handled disease index)/(contrast disease index)] * 100%
6, result and analysis
Table 1-1 anti-plant virus agent control tobacco mosaic virus disease test effect
Remarks: above data are 3 mean values that repeat.
Analyze as can be known from table 1: it is obvious that each handles differences.Catch an illness the cigarette strain at initial stage after anti-plant virus agent is handled, do not have new cigarette strain to catch an illness, disease index descends obviously, and scab obviously disappears, and cigarette strain resistance against diseases obviously strengthens, and has the obvious treatment effect, and relative control effect all is higher than handles D (chemical pesticide).
Table 1-2 different disposal yield of flue-cured tobacco and Ji performance analysis
Handle |
Cell production kg/ sub-district |
Per mu yield kg/ mu |
First-class cigarette ratio % |
Last medium grade cigarette ratio % |
Average price unit/kg |
Per mu yield value unit/mu |
The significance of difference |
Handle A |
??90.28 |
??180.56 |
??38.67 |
??79.45 |
??11.08 |
??2000.61 |
??Aa |
Treatments B |
??87.88 |
??175.76 |
??37.25 |
??76.24 |
??11.02 |
??1936.88 |
??Ab |
Handle C |
??85.1 |
??170.12 |
??37.23 |
??76.21 |
??11.02 |
??1874.72 |
??Ab |
Handle D |
??80.23 |
??160.46 |
??34.79 |
??74.09 |
??10.72 |
??1720.13 |
??Bc |
??CK |
??75.12 |
??150.23 |
??31.24 |
??70.13 |
??10.67 |
??1602.95 |
??Cd |
Remarks: above data are 3 mean values that repeat.
(1) goes up, goes up the medium grade cigarette proportion grading: spray anti-plant virus agent and can obviously improve upward, go up the medium grade cigarette ratio.Handle first-class cigarette of A and last medium grade cigarette ratio and improved 7.43% and 9.32% compared with the control, first-class cigarette of treatments B and last medium grade cigarette ratio have improved 6.01% and 6.11% compared with the control, handle first-class cigarette of C and last medium grade cigarette ratio and have improved 5.99% and 6.08% compared with the control.Handle first-class cigarette of D and last medium grade cigarette ratio and improved 3.55% and 3.96% compared with the control.
(2) per mu yield analysis: handle the A per mu yield and improved 20.19% compared with the control, the treatments B per mu yield has improved 16.99% compared with the control, handles the C per mu yield and has improved 13.24% compared with the control, handles the D per mu yield and has improved 6.81% compared with the control.
(3) per mu yield value is analyzed: handle A per mu yield value and improved 24.81% compared with the control, handle 2 per mu yield values and improved 20.83% compared with the control, handle C per mu yield value and improved 16.95% compared with the control, handle D per mu yield value and improved 7.31% compared with the control.
(4) average price analysis: the average price with processing A is the highest, and the average price of contrast is minimum.
In sum: spray anti-plant virus agent and can obviously reduce production loss, reduce peasant's economic loss.
Embodiment 2
To crush particle diameter and be 40 purpose cream paulownia seed oil cakes packs in the reactor, employing ethanol is extractant, press material: the weight ratio of solvent=1: 20 adds in the material, be under 90 ℃ the condition in extraction temperature, extracted 120 minutes, Separation of Solid and Liquid makes cream paulownia seed oil cake extract behind precipitation.Respectively oxymatrine, bright amine fat, dimethyl sulfoxide (DMSO), cream paulownia seed oil cake extract, higher fatty acid potassium are added in the reactor, the control solution temperature is 80 ℃, and the stirring and dissolving time is 90 minutes; Copper fulvate is added in the reactor, and the control dispersion temperature is 65 ℃, and the dispersed with stirring time is 90 minutes; After cooling, make anti-plant virus agent.Raw materials used and the weight proportion of anti-plant virus agent of preparation is:
50 parts of cream paulownia seed oil cake extracts
50 parts of oxymatrines
50 parts of copper fulvates
50 parts in bright amine fat
60 parts in higher fatty acid potassium
75 parts of dimethyl sulfoxide (DMSO)s
With the anti-plant virus agent agent of the foregoing description 2 preparations, prevent and treat the effect assessment test of the viroses of plant.
1, test objective:
Tobacco mosaic virus disease is one of important disease on the tobacco, and all there is distribution various places, causes serious economic loss to leaf tobacco production, and is therefore significant on producing to the control of tobacco mosaic virus disease.Be the control efficiency of checking anti-plant virus agent, the spy carries out the field control effectiveness test of this chemical control tobacco mosaic virus disease.
2, experimental condition:
2.1 for studying thing: red big
2.2 controlling object: common tobacco mosaic virus disease
2.3 basic condition experimental field
Test is carried out in cigarette ground, field, big flood Pingxiang, Yuanjiang River county.
2.4 spraying time: on June 15th, 2006
3, experimental scheme and method
3.1 reagent agent
|
Chinese/formulation and content |
Production unit |
Test medicine |
The anti-plant virus agent of present embodiment 2 preparations |
|
The contrast medicament |
2% Ningnanmycin aqua |
Seoul Heilongjiang Qianger biochemical technology development Co., Ltd |
3.2 test is handled
Code name |
Test medicine |
Dispenser dosage |
Water consumption (liter/mu) |
??A |
The anti-plant virus agent of present embodiment 2 preparations |
500 times |
??50 |
??B |
The anti-plant virus agent of present embodiment 2 preparations |
1000 times |
??50 |
??C |
The anti-plant virus agent of present embodiment 2 preparations |
2000 times |
??50 |
??D |
2% Ningnanmycin aqua |
500 times |
??50 |
??CK |
Clear water |
??- |
??50 |
3.3 sub-district Pareto diagram
Repeat 1 |
??A |
??B |
??C |
??D |
??CK |
Repeat 2 |
??D |
??C |
??CK |
??B |
??A |
Repeat 3 |
??B |
??CK |
??A |
??C |
??D |
4, test operation
4.1 using method: 5 processing are established in test, 3 repetitions, totally 15 sub-districts.0.5 mu of sub-district area, sub-district are arranged and are pressed table 3.3 randomized arrangement, with " bodyguard " board 16 type backpack blade face manual sprayer even spraying, and equivalent clear water spraying blank district.
4.2 the time of dispenser and number of times
4.2.1 control test: in spray in the June 15 medicine first time, spray in 7 days medicine in every interval amounts to 5 times.
4.3 investigation method
4.3.1 control test: respectively at investigation before the dispenser once, investigation in 7,14,21,28,35 days is 1 time behind the medicine, is unit with the strain, district by district by strain investigation diseased plant rate and disease index.
5, drug effect is calculated
The incidence of disease (%)=(the morbidity strain number/total strain number of investigation) * 100%
Disease index=[∑ (diseased plants at different levels * this disease level value)/(investigating total strain number * 4)] * 100%
Relative control effect %=[(contrast disease index one is handled disease index)/(contrast disease index)] * 100%
6, result and analysis
Table 1-1 anti-plant virus agent control tobacco mosaic virus disease test effect
Remarks: above data are 3 mean values that repeat.
Analyze as can be known from table 1: it is obvious that each handles differences.Catch an illness the cigarette strain at initial stage after anti-plant virus agent is handled, do not have new cigarette strain to catch an illness, disease index descends, and scab obviously disappears, and has the obvious treatment effect, and relative control effect all is higher than handles D (biopesticide).
Table 1-2 different disposal yield of flue-cured tobacco and Ji performance analysis
Handle |
Cell production kg/ sub-district |
Per mu yield kg/ mu |
First-class cigarette ratio % |
Last medium grade cigarette ratio % |
Average price unit/kg |
Per mu yield value unit/mu |
The significance of difference |
Handle A |
??87.7 |
??175.4 |
??46.12 |
??89.26 |
??11.12 |
??1950.45 |
??Aa |
Treatments B |
??80.6 |
??161.2 |
??42.23 |
??86.38 |
??11.08 |
??1786.1 |
??Bb |
Handle C |
??79.9 |
??159.8 |
??42.14 |
??86.27 |
??10.98 |
??1754.6 |
??Bb |
Handle D |
??75.35 |
??150.7 |
??38.58 |
??85.78 |
??10.39 |
??1565.77 |
??Bc |
??CK |
??74.1 |
??148.2 |
??33.27 |
??78.98 |
??9.67 |
??1433.1 |
??De |
Remarks: above data are 3 mean values that repeat.
(1) goes up, goes up the medium grade cigarette proportion grading: spray anti-plant virus agent and can obviously improve upward, go up the medium grade cigarette ratio.Handle first-class cigarette of A and last medium grade cigarette ratio and improved 12.85% and 10.28% compared with the control, first-class cigarette of treatments B and last medium grade cigarette ratio have improved 8.96% and 7.4% compared with the control, handle first-class cigarette of C and last medium grade cigarette ratio and improved 8.87% and 7.29% compared with the control, handle first-class cigarette of D and last medium grade cigarette ratio and improved 5.31% and 6.8% compared with the control.
(2) per mu yield analysis: handle the A per mu yield and improved 18.35% compared with the control, the treatments B per mu yield has improved 8.77% compared with the control, handles the C per mu yield and has improved 7.83% compared with the control, handles the D per mu yield and has improved 1.69% compared with the control.
(3) per mu yield value is analyzed: handle A per mu yield value and improved 36.1% compared with the control, treatments B per mu yield value has improved 24.63% compared with the control, handles C per mu yield value and has improved 22.43% compared with the control, handles D per mu yield value and has improved 9.26% compared with the control.
(4) average price analysis: the average price with processing A is the highest, and the average price of contrast is minimum.
In sum: spray anti-plant virus agent and can obviously reduce production loss, reduce peasant's economic loss.
Embodiment 3
To crush particle diameter and be 30 purpose cream paulownia seed oil cakes and pack in the reactor, employing ethanol is extractant, presses material: the weight ratio of solvent=1: 10 adds in the material, be under 70 ℃ the condition in extraction temperature, came together 80 minutes, Separation of Solid and Liquid makes cream paulownia seed oil cake extract behind precipitation.Respectively sophocarpine, abscisic acid, ethanol, cream paulownia seed oil cake extract, neopelex are added in the reactor, the control solution temperature is 65 ℃, and the stirring and dissolving time is 55 minutes; Fulvic acid manganese is added in the reactor, and the control dispersion temperature is 60 ℃, and the dispersed with stirring time is 55 minutes; After cooling, make anti-plant virus agent.Raw materials used and the weight proportion of anti-plant virus agent of preparation is:
20 parts of cream paulownia seed oil cake extracts
20 parts of sophocarpine
30 parts in fulvic acid manganese
25 parts of abscisic acids
35 parts of neopelexes
45 parts of ethanol
With the anti-plant virus agent agent of the foregoing description 3 preparations, prevent and treat the effect assessment test of the viroses of plant.
1, test objective:
Tobacco mosaic virus disease is one of important disease on the tobacco, and all there is distribution various places, causes serious economic loss to leaf tobacco production, and is therefore significant on producing to the control of tobacco mosaic virus disease.Be the control efficiency of checking anti-plant virus agent, the spy carries out the field control effectiveness test of this chemical control tobacco mosaic virus disease.
2, experimental condition:
2.1 for studying thing: red big
2.2 controlling object: common tobacco mosaic virus disease
2.3 basic condition experimental field
Test is carried out in the precious China in Nan Jian county demonstration cigarette ground, mountain region, village.
2.4 spraying time: on June 20th, 2007
3, experimental scheme and method
3.1 reagent agent
|
Chinese/formulation and content |
Production unit |
Test medicine |
The anti-plant virus agent of present embodiment 3 preparations |
|
The contrast medicament |
0.5% amino-oligosaccharide aqua |
Guangxi Beihai Guofa Ocean Biological Pesticide Co., Ltd. |
3.2 test is handled
Code name |
Test medicine |
Dispenser dosage |
Water consumption (liter/mu) |
??A |
The anti-plant virus agent of present embodiment 3 preparations |
500 times |
??50 |
??B |
The anti-plant virus agent of present embodiment 3 preparations |
1000 times |
??50 |
??C |
The anti-plant virus agent of present embodiment 3 preparations |
2000 times |
??50 |
??D |
0.5% amino-oligosaccharide aqua |
500 times |
??50 |
??CK |
Clear water |
??- |
??50 |
3.3 sub-district Pareto diagram
Repeat 1 |
??A |
??B |
??C |
??D |
??CK |
Repeat 2 |
??D |
??C |
??CK |
??B |
??A |
Repeat 3 |
??B |
??CK |
??A |
??C |
??D |
4, test operation
4.1 using method: 5 processing are established in test, 3 repetitions, totally 15 sub-districts.0.5 mu of sub-district area, sub-district are arranged and are pressed table 3.3 randomized arrangement, with " bodyguard " board 16 type backpack blade face manual sprayer even spraying, and equivalent clear water spraying blank district.
4.2 the time of dispenser and number of times
4.2.1 control test: in spray in the June 20 medicine first time, spray in 7 days medicine in every interval amounts to 5 times.
4.3 investigation method
4.3.1 control test: respectively at investigation before the dispenser once, investigation in 7,14,21,28,35 days is 1 time behind the medicine, is unit with the strain, district by district by strain investigation diseased plant rate and disease index.
5, drug effect is calculated
The incidence of disease (%)=(the morbidity strain number/total strain number of investigation) * 100%
Disease index=[∑ (diseased plants at different levels * this disease level value)/(investigating total strain number * 4)] * 100%
Relative control effect %=[(contrast disease index one is handled disease index)/(contrast disease index)] * 100%
6, result and analysis
Table 1-1 anti-plant virus agent control tobacco mosaic virus disease test effect
Remarks: above data are 3 mean values that repeat.
Analyze as can be known from table 1: it is obvious that each handles differences.Catch an illness the cigarette strain at initial stage after anti-plant virus agent is handled, do not have new cigarette strain to catch an illness, disease index descends, and scab obviously disappears, and has the obvious treatment effect, and relative control effect all is higher than handles D (biopesticide).
Table 1-2 different disposal yield of flue-cured tobacco and Ji performance analysis
Handle |
Cell production kg/ sub-district |
Per mu yield kg/ mu |
First-class cigarette ratio % |
Last medium grade cigarette ratio % |
Average price unit/kg |
Per mu yield value unit/mu |
The significance of difference |
Handle A |
??109.92 |
??219.84 |
??38.32 |
??86.60 |
??11.18 |
??2458.71 |
??Aa |
Treatments B |
??97.88 |
??195.76 |
??37.45 |
??84.91 |
??11.32 |
??2216.00 |
??Bb |
Handle C |
??94.50 |
??189 |
??36.34 |
??79.89 |
??11.10 |
??2097.90 |
??Bc |
Handle D |
??92.25 |
??184.5 |
??33.56 |
??77.86 |
??10.80 |
??1992.60 |
??Cd |
??CK |
??92.54 |
??185.08 |
??30.67 |
??75.23 |
??9.46 |
??1750.86 |
??De |
Remarks: above data are 3 mean values that repeat.
(1) last, last medium grade cigarette proportion grading: spray anti-plant virus agent and can obviously improve first-class, last medium grade cigarette ratio.Handle first-class cigarette of A and last medium grade cigarette ratio and improved 7.65% and 11.37% compared with the control, first-class cigarette of treatments B and last medium grade cigarette ratio have improved 6.8% and 9.7% compared with the control, handle first-class cigarette of C and last medium grade cigarette ratio and improved 5.7% and 4.6% compared with the control, handle first-class cigarette of D and last medium grade cigarette ratio and improved 2.9% and 2.6% compared with the control.
(2) per mu yield analysis: handle the A per mu yield and improved 18.78% compared with the control, the treatments B per mu yield has improved 5.77% compared with the control, handles the C per mu yield and improves 2.12% compared with the control, handles the D per mu yield and reduces by 0.31% compared with the control.
(3) per mu yield value is analyzed: handle A per mu yield value and improved 40.42% compared with the control, treatments B per mu yield value has improved 26.57% compared with the control, handles C per mu yield value and has improved 19.83% compared with the control, handles D per mu yield value and has improved 13.81% compared with the control.
(4) average price analysis: the average price with treatments B is the highest, and the average price of contrast is minimum.
In sum: spray anti-plant virus agent and can obviously reduce production loss, reduce peasant's economic loss.
Embodiment 4
To crush particle diameter and be 30 purpose cream paulownia seed oil cakes and pack in the reactor, employing ethanol is extractant, presses material: the weight ratio of solvent=1: 20 adds in the material, be under 60 ℃ the condition in extraction temperature, extracted 90 minutes, Separation of Solid and Liquid makes cream paulownia seed oil cake extract behind precipitation.Respectively N-Oxysophocarpine, sophoramine, abscisic acid, compound sodium nitrophenolate, water, dimethyl sulfoxide (DMSO), cream paulownia seed oil cake extract, neopelex, zinc naphthenate are added in the reactor, the control solution temperature is 70 ℃, and the stirring and dissolving time is 50 minutes; Sodium fulvic acid, fulvic acid zinc are added in the reactor, and the control dispersion temperature is 60 ℃, and the dispersed with stirring time is 60 minutes; After cooling, make anti-plant virus agent.Raw materials used and the weight proportion of anti-plant virus agent of preparation is:
30 parts of cream paulownia seed oil cake extracts
15 parts of N-Oxysophocarpines
15 parts of sophoramines
10 parts of sodium fulvic acids
10 parts on fulvic acid zinc
10 parts of compound sodium nitrophenolates
15 parts in nucleotide
20 parts of neopelexes
20 parts of zinc naphthenates
30 parts in water
20 parts of dimethyl sulfoxide (DMSO)s
With the anti-plant virus agent agent of the foregoing description 4 preparations, prevent and treat the effect assessment test of the viroses of plant.
1, test objective:
The chilli pepper mosaic virus disease is one of important disease on the capsicum, and all there is distribution various places, produces to capsicum and causes serious economic loss, and is therefore significant on producing to the control of chilli pepper mosaic virus disease.Be the control efficiency of checking anti-plant virus agent, the spy carries out the field control effectiveness test of this chemical control chilli pepper mosaic virus disease.
2, experimental condition:
2.1 for studying thing: capsicum (peppery special blue or green)
2.2 controlling object: cucumber mosaic virus
2.3 basic condition experimental field
Test is carried out in mountain region, Qiubei County bark village.
2.4 spraying time: on July 20th, 2007
3, experimental scheme and method
3.1 reagent agent
|
Chinese/formulation and content |
Production unit |
Test medicine |
The anti-plant virus agent of present embodiment 4 preparations |
|
|
Chinese/formulation and content |
Production unit |
The contrast medicament |
2.8% alkanol copper sulphate suspension emulsion |
Pearl new technology trial (demonstration) plant, Henan, Zhengzhou, Henan Province |
3.2 test is handled
Code name |
Test medicine |
Dispenser dosage |
Water consumption (liter/mu) |
??A |
The anti-plant virus agent of present embodiment 4 preparations |
500 times |
??50 |
??B |
The anti-plant virus agent of present embodiment 4 preparations |
1000 times |
??50 |
??C |
The anti-plant virus agent of present embodiment 4 preparations |
2000 times |
??50 |
??D |
2.8% alkanol copper sulphate suspension emulsion |
500 times |
??50 |
??CK |
Clear water |
??- |
??50 |
3.3 sub-district Pareto diagram
Repeat 1 |
??A |
??B |
??C |
??D |
??CK |
Repeat 2 |
??D |
??C |
??CK |
??B |
??A |
Repeat 3 |
??B |
??CK |
??A |
??C |
??D |
4, test operation
4.1 using method: 5 processing are established in test, 3 repetitions, totally 15 sub-districts.0.5 mu of sub-district area, sub-district are arranged and are pressed table 3.3 randomized arrangement, with " bodyguard " board 16 type backpack blade face manual sprayer even spraying, and equivalent clear water spraying blank district.
4.2 the time of dispenser and number of times
4.2.1 control test: in spray in the July 20 medicine first time, spray in 7 days medicine in every interval amounts to 5 times.
4.3 investigation method
4.3.1 control test: respectively at investigation before the dispenser once, investigation in 7,14,21,28,35 days is 1 time behind the medicine, is unit with the strain, district by district by strain investigation diseased plant rate and disease index.
5, drug effect is calculated
The incidence of disease (%)=(the morbidity strain number/total strain number of investigation) * 100%
Disease index=[∑ (diseased plants at different levels * this disease level value)/(investigating total strain number * 4)] * 100%
Relative control effect %=[(contrast disease index one is handled disease index)/(contrast disease index)] * 100%
6, result and analysis
Table 1-1 anti-plant virus agent control capsicum cucumber mosaic viral disease test effect
Remarks: above data are 3 mean values that repeat.
Analyze as can be known from table 1: it is obvious that each handles differences.Catch an illness the pepper plant at initial stage after anti-plant virus agent is handled, do not have new plant to catch an illness, disease index descends, and scab obviously disappears, and has the obvious treatment effect, and relative control effect all is higher than handles D (chemical pesticide).
Table dried green pepper output of 1-2 different disposal and Ji performance analysis
Handle |
Cell production kg/ sub-district |
Per mu yield kg/ mu |
Average price unit/kg |
Per mu yield value unit/mu |
The significance of difference |
Handle A |
??94.62 |
??189.24 |
??18 |
??3406.32 |
??Aa |
Treatments B |
??85.34 |
??170.68 |
??18 |
??2901.56 |
??Bb |
Handle C |
??80.06 |
??160.12 |
??18 |
??2882.16 |
??Bc |
Handle D |
??76.12 |
??152.24 |
??15 |
??2283.6 |
??Cd |
??CK |
??69.61 |
??139.22 |
??15 |
??2088.3 |
??De |
Remarks: above data are 3 mean values that repeat.
(1) per mu yield analysis: handle the A per mu yield and improved 35.9% compared with the control, the treatments B per mu yield has improved 22.3% compared with the control, handles the C per mu yield and has improved 15% compared with the control, handles the D per mu yield and has improved 9.4% compared with the control.
(2) per mu yield value is analyzed: handle A per mu yield value and improved 63.1% compared with the control, treatments B per mu yield value has improved 38.9% compared with the control, handles C per mu yield value and has improved 38% compared with the control, handles D per mu yield value and has improved 9.4% compared with the control.
(3) average price analysis: the average price with processing A, B, C is the highest, and the average price of contrast is minimum.
In sum: spray anti-plant virus agent and can obviously reduce production loss, reduce peasant's economic loss.
Embodiment 5
To crush particle diameter and be 20 purpose cream paulownia seed oil cakes and pack in the reactor, employing ethanol is extractant, presses material: the weight ratio of solvent=1: 8 adds in the material, be under 80 ℃ the condition in extraction temperature, extracted 100 minutes, Separation of Solid and Liquid makes cream paulownia seed oil cake extract behind precipitation.Respectively matrine, oxymatrine, sophocarpine, N-Oxysophocarpine, sophoramine, Sophoridine, DCPTA, bright amine fat, abscisic acid, compound sodium nitrophenolate, nucleotide, Nafusaku, o-nitrophenol potassium, p-nitrophenol potassium, water, dimethyl sulfoxide (DMSO), ethanol, cream paulownia seed oil cake extract, fatty alcohol-polyoxyethylene ether, higher fatty acid potassium, neopelex, magnesium naphthenate, sodium metnylene bis-naphthalene sulfonate, lignin benzene sulfonic acid calcium, Tea Saponin are added in the reactor, the control solution temperature is 65 ℃, and the stirring and dissolving time is 40 minutes; Fulvic acid potassium, copper fulvate, fulvic acid manganese are added in the reactor, and the control dispersion temperature is 55 ℃, and the dispersed with stirring time is 30 minutes; After cooling, make anti-plant virus agent.Raw materials used and the weight proportion of anti-plant virus agent of preparation is:
35 parts of cream paulownia seed oil cake extracts
5 parts of matrines
5 parts of oxymatrines
5 parts of sophocarpine
5 parts of N-Oxysophocarpines
5 parts of sophoramines
5 parts of Sophoridines
10 parts of fulvic acid potassiums
5 parts of copper fulvates
10 parts in fulvic acid manganese
5 parts of DCPTAs
5 parts in bright amine fat
3 parts of abscisic acids
4 parts of compound sodium nitrophenolates
5 parts in nucleotide
3 parts of Nafusakus
5 parts in o-nitrophenol potassium
1 part in p-nitrophenol potassium
5 parts of fatty alcohol-polyoxyethylene ether
5 parts in higher fatty acid potassium
5 parts of neopelexes
5 parts of magnesium naphthenates
5 parts of sodium metnylene bis-naphthalene sulfonates
5 parts of lignin benzene sulfonic acid calcium
5 parts of Tea Saponins
40 parts in water
10 parts of dimethyl sulfoxide (DMSO)s
10 parts of ethanol
With the anti-plant virus agent agent of the foregoing description 5 preparations, prevent and treat the effect assessment test of the viroses of plant.
1, test objective:
The tomato mosaic virus is one of important disease on the tomato, and all there is distribution various places, and production causes serious economic loss to tomato, and is therefore significant on producing to the control of tomato mosaic virus.Be the control efficiency of checking anti-plant virus agent, the spy carries out the field control effectiveness test of this chemical control tomato mosaic virus.
2, experimental condition:
2.1 for studying thing: tomato (bright red No.)
2.2 controlling object: tobacco mosaic virus
2.3 basic condition experimental field
Test is carried out in the yellow lump of Yuanmou County village green house.
2.4 spraying time: on September 1st, 2007
3, experimental scheme and method
3.1 reagent agent
|
Chinese/formulation and content |
Production unit |
Test medicine |
The anti-plant virus agent of present embodiment 5 preparations |
|
The contrast medicament |
20% moroxydine second copper wetting powder |
Heilongjiang Province Siyou Chemical Co., Ltd., Qiqihar City |
3.2 test is handled
Code name |
Test medicine |
Dispenser dosage |
Water consumption (liter/mu) |
??A |
The anti-plant virus agent of present embodiment 5 preparations |
500 times |
??50 |
??B |
The anti-plant virus agent of present embodiment 5 preparations |
1000 times |
??50 |
??C |
The anti-plant virus agent of present embodiment 5 preparations |
2000 times |
??50 |
??D |
20% moroxydine second copper wetting powder |
500 times |
??50 |
??CK |
Clear water |
??- |
??50 |
3.3 sub-district Pareto diagram
Repeat 1 |
??A |
??B |
??C |
??D |
??CK |
Repeat 2 |
??D |
??C |
??CK |
??B |
??A |
Repeat 1 |
??A |
??B |
??C |
??D |
??CK |
Repeat 3 |
??B |
??CK |
??A |
??C |
??D |
4, test operation
4.1 using method: 5 processing are established in test, 3 repetitions, totally 15 sub-districts.0.5 mu of sub-district area, sub-district are arranged and are pressed table 3.3 randomized arrangement, with " bodyguard " board 16 type backpack blade face manual sprayer even spraying, and equivalent clear water spraying blank district.
4.2 the time of dispenser and number of times
4.2.1 control test: in spray in September 1 medicine first time, spray in 7 days medicine in every interval amounts to 5 times.
4.3 investigation method
4.3.1 control test: respectively at investigation before the dispenser once, investigation in 7,14,21,28,35 days is 1 time behind the medicine, is unit with the strain, district by district by strain investigation diseased plant rate and disease index.
5, drug effect is calculated
The incidence of disease (%)=(the morbidity strain number/total strain number of investigation) * 100%
Disease index=[∑ (diseased plants at different levels * this disease level value)/(investigating total strain number * 4)] * 100%
Relative control effect %=[(contrast disease index one is handled disease index)/(contrast disease index)] * 100%
6, result and analysis
Table 1-1 anti-plant virus agent control tomato mosaic virus test effect
Remarks: above data are 3 mean values that repeat.
Analyze as can be known from table 1: it is obvious that each handles differences.Catch an illness the tomato plant at initial stage after anti-plant virus agent is handled, do not have new plant to catch an illness, disease index descends, and scab obviously disappears, and has the obvious treatment effect, and relative control effect all is higher than handles D (chemical pesticide).
Table 1-2 different disposal tomato production and Ji performance analysis
Handle |
Cell production kg/ sub-district |
Per mu yield kg/ mu |
Average price unit/kg |
Per mu yield value unit/mu |
The significance of difference |
Handle A |
??6490.58 |
??12981.16 |
??0.32 |
??4153.97 |
??Aa |
Handle |
Cell production kg/ sub-district |
Per mu yield kg/ mu |
Average price unit/kg |
Per mu yield value unit/mu |
The significance of difference |
Treatments B |
??6123.72 |
??12247.44 |
??0.3 |
??3674.23 |
??Bb |
Handle C |
??5841.25 |
??11682.5 |
??0.3 |
??3504.75 |
??Bc |
Handle D |
??4896.23 |
??9792.46 |
??0.25 |
??2448.12 |
??Cd |
??CK |
??4011.64 |
??8023.28 |
??0.2 |
??1604.66 |
??De |
Remarks: above data are 3 mean values that repeat.
(1) per mu yield analysis: handle the A per mu yield and improved 61.8% compared with the control, the treatments B per mu yield has improved 52.7% compared with the control, handles the C per mu yield and has improved 45.6% compared with the control, handles the D per mu yield and has improved 22.1% compared with the control.
(2) per mu yield value is analyzed: handle A per mu yield value and improved 158.9% compared with the control, treatments B per mu yield value has improved 129% compared with the control, handles C per mu yield value and has improved 118.4% compared with the control, handles D per mu yield value and has improved 52.6% compared with the control.
(3) average price analysis: the average price with processing A is the highest, and the average price of contrast is minimum.
In sum: spray anti-plant virus agent and can obviously reduce production loss, reduce peasant's economic loss.