CN101292668A - Cucumber female phyletic male seducing combination and male seducing method - Google Patents
Cucumber female phyletic male seducing combination and male seducing method Download PDFInfo
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- CN101292668A CN101292668A CNA2008100585024A CN200810058502A CN101292668A CN 101292668 A CN101292668 A CN 101292668A CN A2008100585024 A CNA2008100585024 A CN A2008100585024A CN 200810058502 A CN200810058502 A CN 200810058502A CN 101292668 A CN101292668 A CN 101292668A
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Abstract
The invention discloses a male flower-inducing composition of the cucumber gynoecious inbred line and a male flower-inducing method thereof, which pertains to the technical field of selection and breeding of crops. The male flower-inducting composition is composed of 180 to 350mg/L of silver nitrate analytically pure active ingredient of which is no less than 99.8 percent and 1980 to 3850mg/L of sodium thiosulfate analytically pure active ingredient of which is no less than 99 percent; the mass ratio of the silver nitrate to the sodium thiosulfate is 1 to 11. Male flour-inducing processing is carried out when the third main leaf has the diameter of 2 to 4cm during the period of three leaves and one central leaf of cucumber seedlings. The invention can cause the differentiation rate of the male flowers of cucumber gynoecious inbred line variety to reach 85 percent to 95 percent, average pollinated seed number rate of single cucumber to be improved by 19 percent to 65 percent compared with medicament, thus reducing drug misadventures and providing technical guarantee for the expanding propagation of the variety and the high yield of seed production.
Description
Technical field
The present invention relates to crops breeding technique field, be specifically related to a kind of the male method of luring of cucumber female series.
Background technology
Cucumber is Curcurbitaceae (Cucurbitaceous) Cucumis (curcumas) crop, and formal name used at school Curcumas sativa L. has the cultivation history in more than 2000 year in China, reaches 125.3 ten thousand hm to China cucumber plantation in the end of the year 2002 area
2, account for about 10% of the national vegetables gross area.The female series cucumber is because precocity, high yield, parthenocarpy ability liking of breeding expert and peasant by force and extremely.But in the seed selection and the production of assorted generation seed of this type of cucumber variety, parents are female series, do not pollinate by manually luring hero then can't obtain pollen, how obtaining male flower and pollen becomes the key technology that restricts in this type of strain breeding and the production of assorted generation seed.And the hero that lures of female series cucumber is handled the ubiquity symptom of chemical damage in the current production, and the male flower inductivity is below 80%, and pollen is of poor quality, causes poor pollination, has a strong impact on seed production.
The present invention has obtained good the luring male composition and lure male method of a kind of effect by luring the screening test of male agent, can make female series cucumber male flower differentiation rate reach 85%-95%, and pollen quantity and quality obviously improve.
Summary of the invention
The purpose of this invention is to provide the male composition of luring of a kind of cucumber female series and lure male method, make the seed selection and the production of hybrid seeds of female series cucumber obtain sufficient male flower and pollen pollination, in seed selection process and the production of assorted generation seed, can't or be difficult to obtain the key technology difficult problem that pollen is pollinated thereby solve the female series cucumber.
The inventive method has overcome and exists poisoning incidence more than 90%, male flower inductivity in the problem below 80% in the male technical research of luring of existing female series cucumber, makes the present invention can produce following beneficial effect:
1, make the male flower differentiation rate of female series cucumber reach 85%-95%, average single melon seed number comparison of pollination improves 19-65% according to medicament, and pollen quantity and quality obviously improve.
2,, and reduced the generation of poisoning because the present invention can make the male flower differentiation rate of cucumber female series strain reach 85%-95%.Therefore, the present invention for this reason veriety expand numerous and the production of hybrid seeds obtains high yield technical guarantee is provided.
One of technical scheme of the present invention is the male composition of luring of a kind of cucumber female series, and this lures male composition to be made up of the mixed liquor of following component and content:
Effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is 180-350mg/L, the concentration of sodium thiosulfate is 1980-3850mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
The present invention more preferably male composition that lures of cucumber female series is made up of the mixed liquor of following component and content:
Effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is that the concentration of 190-300mg/L, sodium thiosulfate is 2090-3300mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
The male composition that lures of the preferred cucumber female series of the present invention is made up of the mixed liquor of following component and content:
Effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is that the concentration of 200mg/L and sodium thiosulfate is 2200mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
Two of technical scheme of the present invention is to lure male composition to the male method of luring of cucumber female series with of the present invention, and this method is by luring male composition, lure male composition preparation, lure male handle and 4 steps of points for attention are formed, its concrete steps are as follows:
One, lure male composition: this composition that lures male composition is that effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is 180-350mg/L, the concentration of sodium thiosulfate is 1980-3850mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
Two, lure the preparation of male composition: in the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 ratio, and the silver nitrate weight that takes by weighing desired concn earlier is dissolved in the 250ml distilled water; Taking by weighing corresponding sodium thiosulfate weight again is dissolved in the 750ml distilled water; Treat to pour liquor argenti nitratis ophthalmicus in hypo solution mixing after two kinds of medicaments fully dissolve, last adding distil water is settled to 5L.If lure the consumption of male composition little or consumption is big in once using, then the consumption that reduces or increase solute and solvent in proportion according to aforementioned compound method is prepared, the concentration after the preparation with lure male composition concentration consistent.The weight that claims composition with analytical balance.
Three, lure male the processing: when cucumber seedling 3 leaves 1 heart, the 3rd open and flat back of true leaf diameter is that 2-4cm begins to handle; Above-mentionedly lure male composition now with the current, the little watering can that atomizing is good is to growing point and spire spraying, and the tender tissue of whole plant is wanted fully evenly moistening but do not dripped; When luring male the processing, relative air humidity is not less than 70%; If humidity is too little, must water in preceding 1 hour in processing, treat to handle again after seedling leaves does not have dew; Fine day between the lights or the sun set and handled in preceding 30 minutes, but the cloudy day whole day handle, guarantee to handle the back and can not tan by the sun by the sun within 3-4 hour; Rain after the processing of outdoor cropping and must handle again; Each time interval 3-4 days of handling are handled to pollinating always and are finished.
Four, points for attention:
1, the concentration of Treatment Solution must proportion speed, during preparation if the solution muddiness then needs preparation again.
2, in use remaining soup can not be used for handling next time.
3, handling the solvent that uses must be distilled water, can not prepare with sewage or running water.
Whole strain tender tissue is squirted evenly, guarantee that medicament fully absorbs.
5, to strengthen the plant rich water quality management during luring male the processing, guarantee the plant normal growth, reach the treatment effect of expection, will keep the field soil relative moisture of 70%-85% during will noting especially handling.Be lower than 70% as field soil relative moisture before luring male the processing, will water in preceding 1 hour in processing.
6, two kinds when luring male composition to mix, must be that liquor argenti nitratis ophthalmicus is poured in the hypo solution, and hypo solution can not be poured in the liquor argenti nitratis ophthalmicus.
Embodiment
Further specify the present invention below in conjunction with embodiment, but embodiment is not construed as limiting protection scope of the present invention only for explanation.
Embodiment 1
The female series cucumber parents KJ733 that test material selects for use Syngenta Co.,Ltd to provide, test procedure is as follows:
One, lure male composition:
Test is provided with 8 processing, and each is handled and handles cucumber seedling 200 strains respectively, and each is handled and lures the composition of male composition and concentration to see the following form 1.
Lure the composition and the concentration proportioning table of male combination in each processing of table 1
Two, lure the preparation of male composition
Press the mass ratio of two kinds of medicaments in the table 1, the silver nitrate weight that takes by weighing earlier desired concn with analytical balance respectively is dissolved in the 250ml distilled water; Taking by weighing corresponding sodium thiosulfate weight more respectively is dissolved in the 750ml distilled water; Treat to pour liquor argenti nitratis ophthalmicus in hypo solution mixing after two kinds of medicaments fully dissolve, last adding distil water is settled to 5L, is mixed with each hero of handling that lures and makes up liquid.
Three, test method
When cucumber seedling 3 leaves 1 heart, begin to handle when diameter was for 2-4cm after the 3rd true leaf of cucumber seedling was open and flat, the little watering can that atomizing is good is to growing point and spire spraying, lure male medicament will make the tender tissue of whole plant fully evenly moistening but do not drip, handled once in every 3-4 days, and handled pollination always and finish.
More than 8 kinds of processing to keep field soil relative moisture 70%-85%, be lower than 70% as field soil humidity before luring male the processing, water in preceding 1 hour in processing; Air humidity is not less than 70%, if humidity is too little, must water in preceding 1 hour in processing, treats to handle after seedling leaves does not have dew again.8 kinds lure the male medicament of handling all is now with the current.
Fine day between the lights or the sun set and handled in preceding 30 minutes, but the cloudy day whole day handle, guarantee to handle the back and can not tan by the sun by the sun within 3-4 hour; Rain after the processing of outdoor cropping and must handle again.
Four, result of the test (seeing Table 2)
1, poisoning investigation result
Random sampling 50 strains investigation, the survey showed that:
Handling 1 processing back does not have poisoning substantially, and leaf color deepens, but plant strain growth is had no adverse effects;
Handling 2 processing backs does not have poisoning substantially, and leaf color deepens, but plant strain growth is had no adverse effects;
After handling 3 processing 6% plant generation poisoning is arranged, reveal any symptoms is: partial blade slightly curls, and plant strain growth is had no adverse effects.
Handle 4 and have 20% plant after handling and have poisoning to take place, reveal any symptoms is: partial blade curls, and the normal plant of internode shortens to some extent, and plant strain growth is had no adverse effects;
Handle 5 and have 30% plant after handling and have poisoning to take place, reveal any symptoms is: partial blade curls, and internode shortens, and plant strain growth is had no adverse effects;
Handle 6 and have 40% plant after handling and have poisoning to take place, reveal any symptoms is: partial blade curls, and internode shortens, and plant strain growth is had certain influence, but the loose powder of normally blooming;
Handle 7 and have 55% plant after handling and have poisoning to take place, reveal any symptoms is: most of leaf rolling, and internode shortens, and is bigger to the influence of plant normal growth, but extenuates to some extent in growth middle and later periods poisoning;
Handle 8 and have 100% plant after handling and have poisoning to take place, reveal any symptoms is: most blades seriously curl, and internode shortens obviously, influences the plant normal growth, and plant early ageing is serious.
2, male flower differentiation rate
By random sampling 50 strains, 1-20 joint position of every strain investigation be totally 20 joints, and the result shows as:
Handling 1, to handle back male flower differentiation joint rate be 15%, and majority be that pair property are spent, and the maximum male flower numbers of single-unit are 2-3;
Handle 2 processing back male flower differentiation rates and reach 85%, average single-unit male flower differentiation number is 8;
Handle 3 and handle back male flower differentiation rate 93%, average single-unit male flower differentiation number is 11;
The 4 processing back male flower differentiation joint rates of handling reach 95%, and average single-unit male flower differentiation number is 13;
The 5 processing back male flower differentiation joint rates of handling reach 95%, and average single-unit male flower differentiation number is 17;
The 6 processing back male flower differentiation joint rates of handling reach 95%, and average single-unit male flower differentiation number is 15;
Male flower differentiation joint rate after processing 7 is handled reaches 95%, and average single-unit male flower differentiation number is 16;
Male flower differentiation joint rate after processing 8 is handled reaches 98%, and average single-unit male flower differentiation number is 11.
3, pollination effect
At initial bloom stage, the female flower of the plant of being untreated is won the male flower of 8 kinds of processing respectively and pollinated, treat after the maturation situation of setting seeds to be investigated, investigate 50 fruits altogether, the result shows:
Handling 1 average single melon seed number of handling the back pollination is 170, but male flower wretched insufficiency during pollination;
Handling 2 average single melon seed number of handling the back pollination is 178, and the male flower supply is sufficient substantially during pollination;
Handling 3 average single melon seed number of handling the back pollination is 216, male flower abundance during pollination;
Handling 4 average single melon seed number of handling the back pollination is 217, male flower abundance during pollination;
Handling 5 average single melon seed number of handling the back pollination is 220, male flower abundance during pollination;
Handling 6 average single melon seed number of handling the back pollination is 215, male flower abundance during pollination;
Handling 7 average single melon seed number of handling the back pollination is 218, male flower abundance during pollination;
Handling 8 average single melon seed number of handling the back pollination is 219, because poisoning is heavy, plant early ageing is serious, and the male flower supply is few during pollination, and supply time is short.
Above-mentioned result of the test shows: handle with the inventive method, lure that the silver nitrate drug concentration is 1980-3850mg/L in the concentration of 180-350mg/L, sodium thiosulfate in the male composition, the mass ratio of silver nitrate and sodium thiosulfate is that male effect of 1: 11 o'clock lure and pollination effect are all more satisfactory in the mixed liquor, and pollination flower amount is sufficient.Wherein best to lure silver nitrate drug concentration in the male composition to be that the concentration of 200mg/L and sodium thiosulfate is handled resultant effect when being 2200mg/L.Do not have poisoning to produce when the silver nitrate drug concentration is lower than 180mg/L in the male composition though lure, the male flower supply is obviously not enough; Lure when the silver nitrate drug concentration is higher than 180mg/L in the male composition, along with luring male composition concentration to increase, the symptom of chemical damage of cucumber plant progressively increases the weight of; Lure that the silver nitrate drug concentration is higher than after the 350mg/L in the male composition, the cucumber plant symptom of chemical damage is very obvious, has a strong impact on the plant normal growth, and the male flower supply is few during pollination, and supply time is short.Therefore, processing 2, processing 3, processing 4, processing 5, processing 6, processing 7 are 6 the comparatively desirable processing in the present embodiment.
Embodiment 2
The female series cucumber parents KJ733 that test material selects for use Syngenta Co.,Ltd to provide, 3 processing are adopted in test, and 3 processing are all in contrast.Handle 1 and be distilled water contrast (CK), handle 2, handle 3 for luring male agent gibberellin GA for luring male agent silver nitrate.
One, the component that lures male composition and the content of 3 processing are as follows:
Handle 1: lure male medicament to select distilled water for use.
Handle 2: lure male medicament to select for use effective ingredient to be no less than 99.8% silver nitrate and analyze purely, be mixed with the concentration (300mg/L of silver nitrate is the optium concentration of handling) of 300mg/L according to a conventional method.
Handle 3: lure male medicament to select gibberellin GA for use, be mixed with the concentration (300mg/L of gibberellin GA is the optium concentration of handling) of 300mg/L according to a conventional method.
Below respectively lure the male medicament of handling to comprise that it all is now with the current that distilled water is handled, and handles cucumber seedling 200 strains respectively.
Two, test method
(1) processing 1 and processing 2 begin to handle when diameter was for 2-4cm after the 3rd true leaf of cucumber seedling was open and flat when cucumber seedling 3 leaves 1 heart, shine the inventive method and handle once in every 3-4 days, handling the end of pollinating always.
Handle 2 and handle cucumber seedlings, lure male medicament will make the tender tissue of whole plant fully evenly moistening but do not drip.
(2) handle 3 and after the cucumber seedling cotyledon flattens, begin to handle, respectively cotyledon period, 1 leaf, 3 leaves, 5 leaves, 7 leaves, the 9 leaf phase coprocessing of cucumber seedling 6 times.
Three, result of the test
1, poisoning investigation result
Random sampling 50 strains investigation, the survey showed that:
Handle 1 no poisoning.
Handling 2 plant that have 95% or more after handling has the poisoning shape to produce, and reveal any symptoms is: most of leaf-shrinkage, curling, the obvious thickening of blade, internode obviously shortens, the plant growing way a little less than;
Handle 3 and have 95% plant after handling and have poisoning to take place, reveal any symptoms is: all leaf-shrinkages, curl, blade obviously diminishes, attenuation, and internode is elongated, the plant growing way a little less than.
2, male flower differentiation rate
By random sampling 50 strains, 1-20 joint position of every strain investigation be totally 20 joints, result's performance:
Handling 1 processing back male flower differentiation rate is 0%, and the female flower differentiation rate is 100%;
Male flower differentiation joint rate after processing 2 is handled reaches 63%, and single-unit male flower differentiation number is 5, and two property flowers have 5;
Male flower differentiation joint rate after processing 3 is handled reaches 75%, and single-unit male flower differentiation number is 8, and two property flowers have 3.
3, pollination effect
At initial bloom stage, the female flower of the plant of being untreated is won the male flower of processing 2 and processing 3 respectively and pollinated, treat after the maturation situation of setting seeds to be investigated, investigate 50 fruits altogether, the result shows:
Handle 1 and do not obtain the male flower pollination, so there is not seed;
Handle 2 after handling 180;
After processing 3 is handled is 130.
(result of the test is referring to table 2)
Table 2 embodiment 1 and embodiment 2 various processing are to the male effect of luring of cucumber female series
Above-mentioned test explanation: the pollen quality of handling with the inventive method is better than other processing.The present invention can make the male flower differentiation rate of cucumber female series kind reach 85-95%, single melon seed number of pollination (mean value of processing 7 is arrived in the processing 2 in embodiment in the table 2 1) comparison improves 19-65% according to medicament, reduce poisoning and take place, the veriety expansion is numerous for this reason provides technical guarantee with production of hybrid seeds acquisition high yield.
Claims (7)
1, a kind of cucumber female series lure male composition, it is characterized in that: this lures male composition to be made up of the mixed liquor of following component and content:
Effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is that the concentration of 180-350mg/L, sodium thiosulfate is 1980-3850mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
2, according to claim 1ly lure male composition, it is characterized in that: this lures male composition to be made up of the mixed liquor of following component and content:
Effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is that the concentration of 190-300mg/L, sodium thiosulfate is 2090-3300mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
3, according to claim 2ly lure male composition, it is characterized in that: this lures male composition to be made up of the mixed liquor of following component and content:
Effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is that the concentration of 200mg/L, sodium thiosulfate is 2200mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor.
4, according to claim 1 or claim 2 or claim 3 is described lures male composition to the male method of luring of cucumber female series, by luring male composition, lure male composition preparation, lure male handle and 4 steps of points for attention are formed, it is characterized in that:
(1) luring the composition of male composition is that effective ingredient is no less than 99.8% silver nitrate and analyzes pure and mild effective ingredient and be no less than 90.0% sodium thiosulfate and analyze pure, the concentration of its silver nitrate is that the concentration of 180-350mg/L, sodium thiosulfate is 1980-3850mg/L, and the mass ratio of silver nitrate and sodium thiosulfate is 1: 11 in the mixed liquor;
(2) in the preparation that lures male composition, be 1: 11 ratio in the mass ratio of silver nitrate and sodium thiosulfate, the silver nitrate weight that takes by weighing desired concn with analytical balance earlier is dissolved in the 250ml distilled water; Taking by weighing corresponding sodium thiosulfate weight again is dissolved in the 750ml distilled water; Treat to pour liquor argenti nitratis ophthalmicus in hypo solution mixing after two kinds of medicaments fully dissolve, last adding distil water is settled to 5L.If lure the consumption of male composition little or consumption is big in once using, then the consumption that reduces or increase solute and solvent in proportion according to aforementioned compound method is prepared, the concentration after the preparation with lure male composition concentration consistent;
(3) in luring male the processing, when cucumber seedling 3 leaves 1 heart, the 3rd open and flat back of true leaf diameter is that 2-4cm begins to handle; Above-mentionedly lure male composition now with the current, the little watering can that atomizing is good is to growing point and spire spraying, and the tender tissue of whole plant is wanted fully evenly moistening but do not dripped; When luring male the processing, relative air humidity is not less than 70%; If humidity is too little, must water in preceding 1 hour in processing, treat to handle again after seedling leaves does not have dew; Fine day between the lights or the sun set and handled in preceding 30 minutes, but the cloudy day whole day handle, guarantee to handle the back and can not tan by the sun by the sun within 3-4 hour; Rain after the processing of outdoor cropping and must handle again; Each time interval 3-4 days of handling are handled to pollinating always and are finished;
(4) to keep field soil relative moisture 70%-85% during luring male the processing; Field soil relative moisture is lower than 70% before luring male the processing, water in preceding 1 hour in processing.
5, method according to claim 4 is characterized in that: when luring male composition to prepare, solution becomes turbid, and prepare again.
6, method according to claim 4 is characterized in that: remainingly in the use lure male composition can not be used for handling next time.
7, method according to claim 4 is characterized in that: two kinds when luring male composition to mix, must be that liquor argenti nitratis ophthalmicus is poured in the hypo solution, and hypo solution can not be poured in the liquor argenti nitratis ophthalmicus.
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| CNA2008100585024A CN101292668A (en) | 2008-06-05 | 2008-06-05 | Cucumber female phyletic male seducing combination and male seducing method |
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| CNA2008100585024A CN101292668A (en) | 2008-06-05 | 2008-06-05 | Cucumber female phyletic male seducing combination and male seducing method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101743903B (en) * | 2009-12-11 | 2012-05-23 | 南京农业大学 | Cultivation method of gynoecial north China type cucumber Nanci No. 1 and application thereof |
| CN103190281A (en) * | 2013-04-25 | 2013-07-10 | 山东省农业科学院蔬菜研究所 | Cucumber female line induced screening method |
| CN104642093A (en) * | 2015-01-24 | 2015-05-27 | 吉林省蔬菜花卉科学研究院 | Method for cultivating cucumbers by inducing male flowers |
| CN105875234A (en) * | 2016-04-29 | 2016-08-24 | 酒泉市华美种子有限责任公司 | Female-line cucumber male induction method |
| CN108633726A (en) * | 2018-02-26 | 2018-10-12 | 广西壮族自治区农业科学院 | A kind of balsam pear gynoecy strain Rapid identification and its method of reserving seed for planting |
-
2008
- 2008-06-05 CN CNA2008100585024A patent/CN101292668A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101743903B (en) * | 2009-12-11 | 2012-05-23 | 南京农业大学 | Cultivation method of gynoecial north China type cucumber Nanci No. 1 and application thereof |
| CN103190281A (en) * | 2013-04-25 | 2013-07-10 | 山东省农业科学院蔬菜研究所 | Cucumber female line induced screening method |
| CN103190281B (en) * | 2013-04-25 | 2014-07-09 | 山东省农业科学院蔬菜研究所 | Cucumber female line induced screening method |
| CN104642093A (en) * | 2015-01-24 | 2015-05-27 | 吉林省蔬菜花卉科学研究院 | Method for cultivating cucumbers by inducing male flowers |
| CN105875234A (en) * | 2016-04-29 | 2016-08-24 | 酒泉市华美种子有限责任公司 | Female-line cucumber male induction method |
| CN108633726A (en) * | 2018-02-26 | 2018-10-12 | 广西壮族自治区农业科学院 | A kind of balsam pear gynoecy strain Rapid identification and its method of reserving seed for planting |
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Open date: 20081029 |