CN103524261A - Composite microbial inoculum fertilizer - Google Patents
Composite microbial inoculum fertilizer Download PDFInfo
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- CN103524261A CN103524261A CN201310436415.9A CN201310436415A CN103524261A CN 103524261 A CN103524261 A CN 103524261A CN 201310436415 A CN201310436415 A CN 201310436415A CN 103524261 A CN103524261 A CN 103524261A
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Abstract
The invention discloses a composite microbial inoculum fertilizer. The invention is characterized in that the fertilizer is prepared from the following components in parts by weight: 6-8 parts of composite microbial inoculum, 25-35 parts of potassium sulfate, 40-55 parts of urea, 35-50 parts of calcium superphosphate, 150-175 parts of biomass power plant ash, 300-330 parts of sawdust, 200-250 parts of biological carbon, 100-115 parts of medical stone powder, 120-125 parts of zeolite powder and 7-9 parts of soil conditioner. The effective components of the composite microbial inoculum comprise Bacillus licheniformis, Bacillus pumilus, Bacillus lateraporus, Bacillus megaterium and silicate bacterium. The composite microbial inoculum is added to the product to improve the microflora distribution and microenvironment in soil; and the abundant generated derivatives chelate or block the direct damage of heavy metals to the tobacco to some extent, and have the function of restoring the polluted soil. The soil conditioner contains bowlder which contains abundant microelements beneficial to the human body, and the microelements are absorbed by the crops and converted through the food chain so as to be beneficial to the human body; and meanwhile, the adsorption component can effectively adsorb and solidify toxic heavy metal elements in soil, reduce the heavy metal elements absorbed by the crops, and enhance the crop yield.
Description
Technical field
The present invention relates to a kind of fertilizer, particularly a kind of complex micro organism fungicide fertilizer.
Background technology
The existing bio-fertilizer of China all be take organic matter as basis, is then equipped with microbial inoculum and inorganic fertilizer mixes.The application of bio-feritlizer in agricultural approved by people gradually.In order extensively to improve the present situation of this traditional bio-feritlizer, investigator is devoted to the biological fertilizer products that exploitation makes new advances, and makes it that crop alimentary can be provided, and can improve soil again; Can also, to disinfecting soil, utilize biological decomposition and eliminate the pollutent in soil, and soil be played to repair simultaneously simultaneously.
Along with the generally application of bio-feritlizer, few places occurred without production licence when producing bio-feritlizer, and production technique falls behind, and with the bacterial classification not being tested and appraised, produces, and effectively bacterial content is low, the miscellaneous bacteria phenomenon such as exceed standard; Some producer also adds chemical fertilizer or other additive of underexercise in bio-feritlizer, make that nitrogen, phosphorus, the potassium content of chemical fertilizer in fertilizer is too high causes effective bacterium dead, thereby lose the effect of bio-fertilizer, cause crop-planting to occur disease and pest etc., cause crop yield to decline.
Summary of the invention
For the problems referred to above, the object of this invention is to provide that a kind of nutrition is reasonable, composition comprehensively, scientific formulation, can improve rapidly comprehensively soil quality, effect lasting, produce effects obviously and improve the complex micro organism fungicide fertilizer of crop yield.
In order to realize object of the present invention, the present invention passes through following scheme implementation:
1, a kind of complex micro organism fungicide fertilizer, it is characterized in that, by the formula of following weight part, made: complex micro organism fungicide 6-8, vitriolate of tartar 25-35, urea 240-255, calcium superphosphate 35-50, biomass power plant ash 150-175, sawdust 300-330, charcoal 200-250, medical stone powder 100-115, zeolite powder 120-125, soil improvement agent 7-9.
2, its effective constituent of described complex micro organism fungicide is bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus megaterium and silicate bacteria, it is characterized in that, in microbial inoculum, the viable count of bacillus licheniformis is 0.5 * 10-1.5 * 10cfu/g, the viable count of bacillus pumilus is 0.2 * 10-0.8 * 10cfu/g, the viable count of bacillus laterosporus is 0.2 * 10-0.8 * 10cfu/g, the viable count of bacillus megaterium is 0.5 * 10-1.5 * 10cfu/g, and the viable count of silicate bacteria is 0.5 * 10-1.5 * 10cfu/g; The preparation process of complex micro organism fungicide is as follows: respectively bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus megaterium and silicate bacteria are carried out after slant culture, shaking table cultivation and fermentor cultivation, the fermented liquid obtaining is mixed, after concentrate drying and get final product, detailed process is as follows:
(1) slant culture: respectively Bacillus licheniformis, bacillus laterosporus, bacillus pumilus and bacillus megaterium and silicate bacteria are inoculated on slant medium under aseptic condition, cultivate 36-48 hour under 28 ± 2 ℃ of conditions;
(2) shaking table is cultivated: the bacterial classification that above-mentioned steps (1) is cultivated is inoculated in respectively liquid nutrient medium under aseptic condition, and at pH6.5-7.0, temperature is under 30 ℃ of conditions, and 140-160r/min shaking table is cultivated 36-48 hour;
(3) fermentor cultivation: the bacterial classification that above-mentioned steps (2) is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, at pH7.5-8.0, tank pressure 0.5kg, temperature is under 30 ℃, ventilation 1:0.8-1.1 condition, cultivate after 48-56 hour, viable count is greater than 1.0 * 10/mL, and 80% thalline changes into gemma tank at present, obtains fermented liquid;
(4) fermented liquid that obtains in step (3) is mixed, after concentrate drying and get final product.
The formula of the slant medium wherein, using in step (1) is as follows: glucose 15g, fish peptone 5g, yeast extract paste 5g, agar 15g, water 1000mL.
The liquid culture based formulas of using in step (2) is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, potassium hydrogen phosphate 0.5g, sal epsom 0.2g, water 1000mL.
The fermentor cultivation based formulas of using in above-mentioned steps (3) is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, water 600kg.
3, described soil improvement agent is made by the raw material of following weight part:
Zeolite 90-100, alcohol ester 12 2-3, nanometer jade powder 3-4, oil foot 4-5, xitix 2-3, oxalic acid 1-2,2-amino-2-methyl-1-propanol 3-4, Tremoliteor Tremoliteasbestos 5-6, sal epsom 2-3, Sodium Selenite 4-5, cuttlefish bone meal 5-6, water are appropriate; Preparation method adds suitable quantity of water by zeolite, Tremoliteor Tremoliteasbestos to grind to form slurry, be heated to 75-80 ℃, add again nanometer jade powder, oil foot, xitix, oxalic acid to continue to grind 1-2 hour, concentrate drying, add again sal epsom, Sodium Selenite, cuttlefish bone meal to stir, last, add other remaining component, granulation, obtains.
4, complex micro organism fungicide fertilizer of the present invention is made by following concrete steps:
(1) first according to the rate of exchange take complex micro organism fungicide 6-8, vitriolate of tartar 25-35, urea 240-255, calcium superphosphate 35-50, biomass power plant ash 150-175, sawdust 300-330, charcoal 200-250, medical stone powder 100-115, zeolite powder 120-125 is stand-by;
(2) complex micro organism fungicide weighing up, vitriolate of tartar, urea, calcium superphosphate, biomass power plant ash, sawdust, charcoal, medical stone powder, zeolite powder are mixed to rear pulverizing, granulation, dry;
(3) in above-mentioned particle, add soil improvement agent, mix, obtain.
The invention has the advantages that:
In complex micro organism fungicide fertilizer of the present invention, rationally, the microbial enzyme system of bacillus megaterium and silicate bacteria combination results is with strong complementarity in contained microbe species collocation, can strengthen the ability of minute phosphorus decomposing and release potassium; Bacillus licheniformis can produce multiple protein enzyme and antagonistic substance and phytokinin and dormin etc., after it and bacillus laterosporus and bacillus pumilus collocation, can strengthen beneficial microorganism protease activity, antagonism and the direct ability of kill pathogenic bacteria.Product of the present invention is by adding complex micro organism fungicide, the distribution of Soil Microorganism fauna, microenvironment are improved, being created in to a certain extent chelating or having blocked the direct harm of heavy metal to tobacco of a large amount of derivatives, has the function that rehabilitating soil pollutes.
In soil improvement agent of the present invention, contain jade composition, wherein contain the Macro and microelements useful to human body, by Crop, through food chain, transform, useful to human body; Meanwhile, wherein contain adsorption component, toxic heavy metal element in energy active adsorption, curing soil, reduces heavy metal element absorbed by crops.Can improve crop yield.
Specific embodiments
Below by specific examples, the present invention is described in detail.
1, the preparation of complex micro organism fungicide
Its effective constituent of described complex micro organism fungicide is bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus megaterium and silicate bacteria, it is characterized in that, in microbial inoculum, the viable count of bacillus licheniformis is 0.5 * 10-1.5 * 10cfu/g, the viable count of bacillus pumilus is 0.2 * 10-0.8 * 10cfu/g, the viable count of bacillus laterosporus is 0.2 * 10-0.8 * 10cfu/g, the viable count of bacillus megaterium is 0.5 * 10-1.5 * 10cfu/g, and the viable count of silicate bacteria is 0.5 * 10-1.5 * 10cfu/g; The preparation process of complex micro organism fungicide is as follows: respectively bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus megaterium and silicate bacteria are carried out after slant culture, shaking table cultivation and fermentor cultivation, the fermented liquid obtaining is mixed, after concentrate drying and get final product, detailed process is as follows:
(1) slant culture: respectively Bacillus licheniformis, bacillus laterosporus, bacillus pumilus and bacillus megaterium and silicate bacteria are inoculated on slant medium under aseptic condition, cultivate 36-48 hour under 28 ± 2 ℃ of conditions;
(2) shaking table is cultivated: the bacterial classification that above-mentioned steps (1) is cultivated is inoculated in respectively liquid nutrient medium under aseptic condition, and at pH6.5-7.0, temperature is under 30 ℃ of conditions, and 140-160r/min shaking table is cultivated 36-48 hour;
(3) fermentor cultivation: the bacterial classification that above-mentioned steps (2) is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, at pH7.5-8.0, tank pressure 0.5kg, temperature is under 30 ℃, ventilation 1:0.8-1.1 condition, cultivate after 48-56 hour, viable count is greater than 1.0 * 10/mL, and 80% thalline changes into gemma tank at present, obtains fermented liquid;
(4) fermented liquid that obtains in step (3) is mixed, after concentrate drying and get final product.
The formula of the slant medium wherein, using in step (1) is as follows: glucose 15g, fish peptone 5g, yeast extract paste 5g, agar 15g, water 1000mL.
The liquid culture based formulas of using in step (2) is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, potassium hydrogen phosphate 0.5g, sal epsom 0.2g, water 1000mL.
The fermentor cultivation based formulas of using in above-mentioned steps (3) is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, water 600kg.
2, the preparation of soil improvement agent
Zeolite 90kg, alcohol ester 12 2kg, nanometer jade powder 3kg, oil foot 4kg, xitix 2kg, oxalic acid 1kg, 2-amino-2-methyl-1-propanol 3kg, Tremoliteor Tremoliteasbestos 5kg, sal epsom 2kg, Sodium Selenite 4kg, cuttlefish bone meal 5kg, water are appropriate; Preparation method adds suitable quantity of water by zeolite, Tremoliteor Tremoliteasbestos to grind to form slurry, be heated to 75-80 ℃, add again nanometer jade powder, oil foot, xitix, oxalic acid to continue to grind 1.5 hours, concentrate drying, add again sal epsom, Sodium Selenite, cuttlefish bone meal to stir, last, add other remaining component, granulation, obtains.
2, the preparation of complex micro organism fungicide fertilizer
(1) first according to the rate of exchange take complex micro organism fungicide 6kg, vitriolate of tartar 25kg, urea 240kg, calcium superphosphate 35kg, biomass power plant ash 150kg, sawdust 300kg, charcoal 200kg, medical stone powder 100kg, zeolite powder 120kg is stand-by;
(2) complex micro organism fungicide weighing up, vitriolate of tartar, urea, calcium superphosphate, biomass power plant ash, sawdust, charcoal, medical stone powder, zeolite powder are mixed to rear pulverizing, granulation, dry;
(3) to the soil improvement agent that adds certain proportioning in above-mentioned particle, mix, obtain.
In order further to grope the effect of this product on farm crop, applicant has carried out the field plot trial of this fertilizer on tomato in March, 2013 to August, contrasts with common mineral manure, now test-results is summarized as follows:
Complex micro organism fungicide fertilizer has to be urged in the growing of tomato, and obvious effect of increasing production, is worth large scale application.
The fertilizer that the present invention makes is equally applicable to grain, linen-cotton, legume crop, oil crops, herbal medicine, tealeaves, tobacco, vegetable melon and fruit etc.
Claims (2)
1. a complex micro organism fungicide fertilizer, it is characterized in that, by the formula of following weight part, made: complex micro organism fungicide 6-8, vitriolate of tartar 25-35, urea 240-255, calcium superphosphate 35-50, biomass power plant ash 150-175, sawdust 300-330, charcoal 200-250, medical stone powder 100-115, zeolite powder 120-125, soil improvement agent 7-9;
Its effective constituent of described complex micro organism fungicide is bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus megaterium and silicate bacteria, it is characterized in that, in microbial inoculum, the viable count of bacillus licheniformis is 0.5 * 10-1.5 * 10 cfu/g, the viable count of bacillus pumilus is 0.2 * 10-0.8 * 10 cfu/ g, the viable count of bacillus laterosporus is 0.2 * 10-0.8 * 10 cfu/g, the viable count of bacillus megaterium is 0.5 * 10-1.5 * 10 cfu/g, and the viable count of silicate bacteria is 0.5 * 10-1.5 * 10 cfu/g; Preparation process is as follows: respectively bacillus licheniformis, bacillus pumilus, bacillus laterosporus, bacillus megaterium and silicate bacteria are carried out after slant culture, shaking table cultivation and fermentor cultivation, the fermented liquid that obtains is mixed, after concentrate drying and get final product;
Described soil improvement agent is made by the raw material of following weight part:
Zeolite 90-100, alcohol ester 12 2-3, nanometer jade powder 3-4, oil foot 4-5, xitix 2-3, oxalic acid 1-2,2-amino-2-methyl isophthalic acid-propyl alcohol 3-4, Tremoliteor Tremoliteasbestos 5-6, sal epsom 2-3, Sodium Selenite 4-5, cuttlefish bone meal 5-6, water are appropriate; Preparation method adds suitable quantity of water by zeolite, Tremoliteor Tremoliteasbestos to grind to form slurry, be heated to 75-80 ℃, add again nanometer jade powder, oil foot, xitix, oxalic acid to continue to grind 1-2 hour, concentrate drying, add again sal epsom, Sodium Selenite, cuttlefish bone meal to stir, last, add other remaining component, granulation, obtains.
2. according to complex micro organism fungicide fertilizer described in claims 1, it is characterized in that, by following concrete steps, made:
(1) first according to the rate of exchange take complex micro organism fungicide 6-8, vitriolate of tartar 25-35, urea 240-255, calcium superphosphate 35-50, biomass power plant ash 150-175, sawdust 300-330, charcoal 200-250, medical stone powder 100-115, zeolite powder 120-125 is stand-by;
(2) complex micro organism fungicide weighing up, vitriolate of tartar, urea, calcium superphosphate, biomass power plant ash, sawdust, charcoal, medical stone powder, zeolite powder are mixed to rear pulverizing, granulation, dry;
(3) in above-mentioned particle, add soil improvement agent, mix, obtain.
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN103864483A (en) * | 2014-02-27 | 2014-06-18 | 杨健 | Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal |
| CN104177194A (en) * | 2014-09-05 | 2014-12-03 | 山东大学 | Method for preparing biochar-based selenium fertilizer |
| CN104230582A (en) * | 2014-09-11 | 2014-12-24 | 红云红河烟草(集团)有限责任公司 | Biological organic fertilizer containing bacillus pumilus |
| CN104447117A (en) * | 2014-12-08 | 2015-03-25 | 临沂中磷生物科技有限公司 | Special composite microorganism fertilizer for grape |
| CN105198549A (en) * | 2015-07-31 | 2015-12-30 | 山东鑫圣源生物工程技术有限公司 | Microbial fertilizer capable of reducing heavy metals and preparation method thereof |
| CN105294268A (en) * | 2015-11-09 | 2016-02-03 | 根力多生物科技股份有限公司 | Compound microorganism fertilizer |
| CN105367206A (en) * | 2014-08-26 | 2016-03-02 | 中国科学院沈阳应用生态研究所 | Compound fertilizer special for bulb flowers and application of compound fertilizer |
| CN105462595A (en) * | 2015-12-21 | 2016-04-06 | 武汉飞喜乐农业科技有限公司 | Acid soil modifier and preparation method thereof |
| CN106083249A (en) * | 2016-06-15 | 2016-11-09 | 陈树平 | A kind of production method of biological bacteria siliceous fertilizer |
| CN106747940A (en) * | 2016-11-25 | 2017-05-31 | 北京光禾生物科技有限公司 | A kind of microbial liquid fertilizer of composite bacteria agent containing biogas slurry and preparation method thereof |
| CN106734159A (en) * | 2016-12-02 | 2017-05-31 | 青岛蔚蓝生物集团有限公司 | A kind of restorative procedure of heavy-metal contaminated soil |
| CN107011087A (en) * | 2017-03-27 | 2017-08-04 | 广西浩昌敏再生资源利用有限公司 | A kind of bio-fertilizer and preparation method thereof |
| CN107244982A (en) * | 2017-07-28 | 2017-10-13 | 眉山市金威特化肥有限公司 | A kind of Tower-type melt granulation high-concentration compound microorganism bacterial fertilizer and preparation method thereof |
| CN109748676A (en) * | 2017-11-03 | 2019-05-14 | 丹阳市皇塘镇蒋墅天意土元专业合作社 | A kind of bio-feritlizer containing microbial bacterial agent |
| CN111778039A (en) * | 2020-08-12 | 2020-10-16 | 浙江省地质调查院 | Preparation of calcined biochar type conditioner and application method of conditioner |
| CN112209778A (en) * | 2020-09-26 | 2021-01-12 | 上海康恒环境修复有限公司 | Novel slag soil conditioner and preparation method thereof |
| CN112661558A (en) * | 2020-03-02 | 2021-04-16 | 首佳优品(北京)国际农业科技有限公司 | Powder for high-quality tomatoes and preparation method and using method thereof |
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Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN103819237B (en) * | 2014-02-07 | 2015-07-29 | 湖南泰谷生物科技股份有限公司 | Tobacco special microorganism is fertile and preparation method thereof |
| CN103864483A (en) * | 2014-02-27 | 2014-06-18 | 杨健 | Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal |
| CN103864483B (en) * | 2014-02-27 | 2015-05-27 | 杨健 | Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal |
| CN105367206A (en) * | 2014-08-26 | 2016-03-02 | 中国科学院沈阳应用生态研究所 | Compound fertilizer special for bulb flowers and application of compound fertilizer |
| CN104177194A (en) * | 2014-09-05 | 2014-12-03 | 山东大学 | Method for preparing biochar-based selenium fertilizer |
| CN104230582A (en) * | 2014-09-11 | 2014-12-24 | 红云红河烟草(集团)有限责任公司 | Biological organic fertilizer containing bacillus pumilus |
| CN104447117A (en) * | 2014-12-08 | 2015-03-25 | 临沂中磷生物科技有限公司 | Special composite microorganism fertilizer for grape |
| CN105198549A (en) * | 2015-07-31 | 2015-12-30 | 山东鑫圣源生物工程技术有限公司 | Microbial fertilizer capable of reducing heavy metals and preparation method thereof |
| CN105294268A (en) * | 2015-11-09 | 2016-02-03 | 根力多生物科技股份有限公司 | Compound microorganism fertilizer |
| CN105462595A (en) * | 2015-12-21 | 2016-04-06 | 武汉飞喜乐农业科技有限公司 | Acid soil modifier and preparation method thereof |
| CN106083249A (en) * | 2016-06-15 | 2016-11-09 | 陈树平 | A kind of production method of biological bacteria siliceous fertilizer |
| CN106747940A (en) * | 2016-11-25 | 2017-05-31 | 北京光禾生物科技有限公司 | A kind of microbial liquid fertilizer of composite bacteria agent containing biogas slurry and preparation method thereof |
| CN106734159A (en) * | 2016-12-02 | 2017-05-31 | 青岛蔚蓝生物集团有限公司 | A kind of restorative procedure of heavy-metal contaminated soil |
| CN107011087A (en) * | 2017-03-27 | 2017-08-04 | 广西浩昌敏再生资源利用有限公司 | A kind of bio-fertilizer and preparation method thereof |
| CN107244982A (en) * | 2017-07-28 | 2017-10-13 | 眉山市金威特化肥有限公司 | A kind of Tower-type melt granulation high-concentration compound microorganism bacterial fertilizer and preparation method thereof |
| CN109748676A (en) * | 2017-11-03 | 2019-05-14 | 丹阳市皇塘镇蒋墅天意土元专业合作社 | A kind of bio-feritlizer containing microbial bacterial agent |
| CN112661558A (en) * | 2020-03-02 | 2021-04-16 | 首佳优品(北京)国际农业科技有限公司 | Powder for high-quality tomatoes and preparation method and using method thereof |
| CN111778039A (en) * | 2020-08-12 | 2020-10-16 | 浙江省地质调查院 | Preparation of calcined biochar type conditioner and application method of conditioner |
| CN112209778A (en) * | 2020-09-26 | 2021-01-12 | 上海康恒环境修复有限公司 | Novel slag soil conditioner and preparation method thereof |
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Application publication date: 20140122 |