CN105198549A - Microbial fertilizer capable of reducing heavy metals and preparation method thereof - Google Patents
Microbial fertilizer capable of reducing heavy metals and preparation method thereof Download PDFInfo
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Abstract
The invention relates to microbial fertilizer, in particular to microbial fertilizer capable of reducing heavy metals and a preparation method thereof. The microbial fertilizer capable of reducing heavy metals is prepared from the following raw materials in parts by weight: 15-25 parts of a compound microbial agent, 9-13 parts of urea, 16-20 parts of monoammonium phosphate, 20-25 parts of potassium sulfate, 10-20 parts of sepiolite, 10-20 parts of activated carbon, 2-5 parts of borax, 2-5 parts of zinc sulfate and 3-5 parts of magnesium sulfate, wherein the compound microbial agent is mainly prepared from the following raw materials by weight: 80-160 kg/ton of soybean cake powder, 48-360 kg/ton of corn starch, 100-280 kg/ton of bran and 30-80 kg/ton of fish meal. According to the fertilizer provided by the invention, through cooperation of the compound microbial agent and other raw materials, absorption of plants to nutrient element is promoted, so that growth of root systems is promoted, the yield is increased, robust plants can be obtained, and stress resistance is enhanced. Toxicity of harmful heavy metals in soil is reduced greatly.
Description
(1) technical field
The present invention relates to microbial fertilizer, be specifically related to a kind of microbial fertilizer reducing heavy metal and preparation method thereof.
(2) background technology
" cadmium rice " event once caused social extensive concern, for we have beaten the alarm bell of soil pollution.In the recent period, Environmental Protection Department and Ministry of Land and Resources combine again and have issued 2005-2013 whole nation Soil Pollution Investigation publication.Result shows, and in factual survey area 6,300,000 square kilometres, national edatope situation totally allows of no optimist, and some areas soil pollution is heavier, and the total exceeding standard rate 16.1% of national soil, soil pollution has become " public hazards " in the many places of China.Under the situation that soil pollution is serious, relevant clay fertilizer expert advice, can by agronomy means as: increase the soil organism, use soil conditioner, adopt biosorption process etc. can passivation, alleviate and even repair the most soil that exceeds standard.In a word, in recent years, China's microbial fertilizer industry development is rapid, and microbial product and the novel microorganism fertilizer in a large number with specific function come out, and in soil, therefore apply microbial fertilizer will be the important development direction that soil ecology is repaired.Microbial fertilizer refers to from soil and plant rhizosphere and is separated the effective bacterium obtained, through screening, be separated, even gene recombination, carry out the optimum combination of bacterial classification, mix with organism through fermentation culture and the microbial preparation prepared.In China, microbial fertilizer has the history in more than 50 years, experienced by the evolution of---bacterial fertilizer---microbial fertilizer from nitragin.At present, microbial fertilizer is mainly divided into microbiobacterial agent, composite microbiological fertilizer and biological organic fertilizer three class.Soil pollution mainly can be divided into inorganic pollutant and organic pollutant, and inorganic pollutant mainly comprises acid, alkali, heavy metal, salt etc.; Organic pollutant mainly includes machine agricultural chemicals, phenols, prussiate, oil, synthetic detergent etc.The ultimate principle of microbial fertilizer rehabilitating soil is under suitable condition (moisture, temperature, pH value etc.), in microorganism species in fertilizer and soil, original beneficial microorganism forms dominant microflora jointly, promote the benign cycle of the elements such as carbon, nitrogen, oxygen in soil ecosystem, thus rehabilitating soil Eco-Environment System, make the stable balance that the ecosystem reaches new.Wherein, the repair mechanisms of microbial fertilizer to inorganic pollutant are after microbial fertilizer enters soil ecosystem, the microorganism probiotic such as aerobic bacteria, anerobe group, by the biological respinse of self, reduce the acidity of soil, improve the pH value of soil, thus reduce the murder by poisoning of harmful heavy metal in soil; Heavy metal can be fixed by the microbial bacteria simultaneously in fertilizer, Active Heavy Metals in soil is impelled to become combination state, form filtering layer and sealing coat, reduce crop to the absorption of heavy metal in soil, thus avoid the objectionable impuritiess such as heavy metal in soil or its degradation production by " soil → plant → human body ", or be indirectly absorbed by the body by " soil → water → human body ", damage healthy.Microbial fertilizer, to the effect in the organic pollutant reparations such as Pesticide Residue in Soil being the control number of times reducing disease and pest, reduces the usage quantity of agricultural chemicals, thus reduces the residual quantity of agricultural chemicals in crop.One, microbial fertilizer can change plough horizon micro-flora, around crop root, form dominant colony, thus the breeding of strongly inhibited pathogenic bacteria, reduce disease and pest frequency; On the other hand, microorganism produces many kinds of substance in its vital movement process, and as hormones, humic-acid kind and antibiotics, these substances stimulate plant growth healthy and strong, strengthen crop self disease resistance ability.The Microbial resources of China have the advantages that microorganism resource is enriched, microbial fertilizer product category is various, have wide range of applications.Abundant Microbial resources provide guarantee for development microbial fertilizer industry, and the more reality of the pollutant kind of feature again in soil that China's microbe species is various is simultaneously corresponding, can multiple pollutant in rehabilitating soil.And microbial fertilizer production cost is low, is beneficial to peasant and accepts, can promote the use of on a large scale in rural area, on the basis jointly used with mineral manure, reduce the usage quantity of mineral manure, play the effect of rehabilitating soil simultaneously.
(3) summary of the invention
The invention provides a kind of microbial fertilizer reducing heavy metal and preparation method thereof, the preparation of this microbial fertilizer is simple, raw material is easy to get, be conducive to promoting.
The present invention is achieved through the following technical solutions:
Reduce a microbial fertilizer for heavy metal, its special character is: be made up of the raw material of following parts by weight:
Complex micro organism fungicide 15-25, urea 9-13, monoammonium phosphate 16-20, potassium sulfate 20-25, sepiolite 10-20, gac 10-20, borax 2-5, zinc sulfate 2-5, magnesium sulfate 3-5,
Wherein, complex micro organism fungicide is made primarily of the raw material of following weight: soybean cake powder 80-160kg/ ton, W-Gum 48-360kg/ ton, wheat bran 100-280kg/ ton, fish meal 30-80kg/ ton.
The preparation method of the microbial fertilizer of reduction heavy metal of the present invention, comprises the following steps:
(1) composite microbial bacteria liquid makes: by soybean cake powder, W-Gum, wheat bran, fish meal, soybean cake powder 80-160kg/ ton in proportion, W-Gum 48-360kg/ ton, wheat bran 100-280kg/ ton, fish meal 30-80kg/ ton, to add in fermentor tank and to inject corresponding water, pH value is adjusted to 7.2-7.8, steam sterilizing, temperature drops to 7-35 DEG C, adds compound gemma bacterial classification, at constant air flow, steady temperature bottom fermentation, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 85-90%, obtains degraded heavy metal composite microbial bacteria liquid;
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:3-1:5, makes the composite microbial bacteria liquid of degraded heavy metal and humic acid mix packing bag fully for subsequent use;
(3) making of the microbial fertilizer of heavy metal is reduced: by complex micro organism fungicide 15-25 part; urea 9-13 part, monoammonium phosphate 16-20 part, potassium sulfate 20-25 part, sepiolite 10-20 part; gac 10-20 part; borax 2-5 part, zinc sulfate 2-5 part, magnesium sulfate 3-5 part; mix with stirrer; vertical crusher is pulverized, and carries out granulation, finally dries, cools, coating, packs reduce the composite microbiological fertilizer of heavy metal by tablets press.
In step (1), steam sterilizing temperature is 115-130 DEG C, pressure 0.07-0.17Mpa, and sterilization time is 10-60min.Fermentation time is 16-20 hour.Steam sterilizing temperature preferably 121 DEG C, pressure 0.12Mp, sterilization time is 30min.
Beneficial effect of the present invention is: fertilizer of the present invention is cooperatively interacted by complex micro organism fungicide and other raw materials, promotes the absorption of Plant To Nutrient element, thus promotes the growth of root system, increase yield, healthy and strong plant, strengthens the inverse property in hole.Continuing to strengthen the reparation of polluted soil by adding complex micro organism fungicide, overcoming traditional chemical improvement agent fine for single or that character is close heavy metal fixed effect, and to the defect that compound heavy-metal contaminated soil reparation can not be completely up to standard; Taken into account economy and environment benefit simultaneously, can large-scale application in the reparation of compound heavy-metal contaminated soil, greatly reduce the murder by poisoning of the harmful heavy metal in soil, microbial fertilizer to the effect in the organic pollutant reparations such as Pesticide Residue in Soil is being the control number of times reducing disease and pest, reduce the usage quantity of agricultural chemicals, thus reduce the residual quantity of agricultural chemicals in crop.And preparation is simple, raw material is easy to get, and is conducive to promoting.
(4) embodiment
Embodiment 1:
Microbial fertilizer and the preparation process thereof of the reduction heavy metal of the present embodiment be,
(1) composite microbial bacteria liquid makes: soybean cake powder 80kg/ ton, W-Gum 48kg/ ton, wheat bran 100kg/ ton, fish meal 50kg/ ton.To add in proportion in fermentor tank and to inject corresponding water, pH value is adjusted to 7.2, steam sterilizing (temperature: 121 DEG C, pressure 0.12Mpa) 30 minutes, temperature dropped to 30 DEG C, adds compound gemma bacterial classification (subtilis: gel-shaped series bacillus: the mass ratio=1:1:1 of Bacillus licheniformis), constant air flow, steady temperature bottom fermentation 16 hours, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 85%, obtains degraded heavy metal composite microbial bacteria liquid.
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:4.Composite microbial bacteria liquid and humic acid is made to mix packing bag fully for subsequent use.
(3) making of the microbial fertilizer of heavy metal is reduced: complex micro organism fungicide 25 parts, 9 parts, urea, monoammonium phosphate 18 parts, potassium sulfate 22 parts, sepiolite 10 parts, gac 20 parts, borax 4 parts, 3 parts, zinc sulfate, 3 parts, magnesium sulfate.Mix with stirrer, vertical crusher is pulverized, and carries out granulation by tablets press, finally dries, cools, the composite microbiological fertilizer of the reduction heavy metal of coating, packaging.
Embodiment 2:
Microbial fertilizer and the preparation process thereof of the reduction heavy metal of the present embodiment be,
(1) composite microbial bacteria liquid makes: soybean cake powder 160kg/ ton, W-Gum 360kg/ ton, wheat bran 280kg/ ton, fish meal 40kg/ ton.To add in proportion in fermentor tank and to inject corresponding water, pH value is adjusted to 7.8, steam sterilizing (temperature: 121 DEG C, pressure 0.12Mpa) 20 minutes, temperature dropped to 7 DEG C, adds compound gemma bacterial classification (subtilis: gel-shaped series bacillus: the mass ratio=1:1:1 of Bacillus licheniformis), constant air flow, steady temperature bottom fermentation 20 hours, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 90%, obtains degraded heavy metal composite microbial bacteria liquid.
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:3.The composite microbial bacteria liquid of degraded heavy metal and humic acid is made to mix packing bag fully for subsequent use.
(3) making of the microbial fertilizer of heavy metal is reduced: complex micro organism fungicide 20 parts, 13 parts, urea, monoammonium phosphate 16 parts, potassium sulfate 25 parts, sepiolite 15 parts, gac 15 parts, borax 5 parts, 2 parts, zinc sulfate, 5 parts, magnesium sulfate.Mix with stirrer, vertical crusher is pulverized, and carries out granulation by tablets press, finally dries, cools, the composite microbiological fertilizer of the reduction heavy metal of coating, packaging.
Embodiment 3:
Microbial fertilizer and the preparation process thereof of the reduction heavy metal of the present embodiment be,
(1) composite microbial bacteria liquid makes: soybean cake powder 120kg/ ton, W-Gum 200kg/ ton, wheat bran 190kg/ ton, fish meal 80kg/ ton.To add in proportion in fermentor tank and to inject corresponding water, pH value is adjusted to 7.4, steam sterilizing (temperature: 121 DEG C, pressure 0.12Mpa) 25 minutes, temperature dropped to 35 DEG C, adds compound gemma bacterial classification (subtilis: gel-shaped series bacillus: the mass ratio=1:1:1 of Bacillus licheniformis), constant air flow, steady temperature bottom fermentation 18 hours, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 88%, obtains the composite microbial bacteria liquid of degraded heavy metal.
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:5.The composite microbial bacteria liquid of degraded heavy metal and humic acid is made to mix packing bag fully for subsequent use.
(3) making of the microbial fertilizer of heavy metal is reduced: complex micro organism fungicide 15 parts, 11 parts, urea, monoammonium phosphate 20 parts, potassium sulfate 20 parts, sepiolite 20 parts, gac 10 parts, borax 2 parts, 5 parts, zinc sulfate, 4 parts, magnesium sulfate.Mix with stirrer, vertical crusher is pulverized, and carries out granulation by tablets press, finally dries, cools, the composite microbiological fertilizer of the reduction heavy metal of coating, packaging.
Embodiment 4:
Microbial fertilizer and the preparation process thereof of the reduction heavy metal of the present embodiment be,
(1) composite microbial bacteria liquid makes: soybean cake powder 120kg/ ton, W-Gum 180kg/ ton, wheat bran 150kg/ ton, fish meal 30kg/ ton.To add in proportion in fermentor tank and to inject corresponding water, pH value is adjusted to 7.5, steam sterilizing (temperature: 115 DEG C, pressure 0.07Mpa) 60 minutes, temperature dropped to 7 DEG C, adds compound gemma bacterial classification (subtilis: gel-shaped series bacillus: the mass ratio=1:1:1 of Bacillus licheniformis), constant air flow, steady temperature bottom fermentation 20 hours, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 90%, obtains the composite microbial bacteria liquid of degraded heavy metal.
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:3.The composite microbial bacteria liquid of degraded heavy metal and humic acid is made to mix packing bag fully for subsequent use.
(3) making of the microbial fertilizer of heavy metal is reduced: complex micro organism fungicide 20 parts, 11 parts, urea, monoammonium phosphate 20 parts, potassium sulfate 25 parts, sepiolite 10 parts, gac 15 parts, borax 4 parts, 4 parts, zinc sulfate, 3 parts, magnesium sulfate.Mix with stirrer, vertical crusher is pulverized, and carries out granulation by tablets press, finally dries, cools, the composite microbiological fertilizer of the reduction heavy metal of coating, packaging.
Embodiment 5:
Microbial fertilizer and the preparation process thereof of the reduction heavy metal of the present embodiment be,
(1) composite microbial bacteria liquid makes: soybean cake powder 80kg/ ton, W-Gum 80kg/ ton, wheat bran 280kg/ ton, fish meal 80kg/ ton.To add in proportion in fermentor tank and to inject corresponding water, pH value is adjusted to 7.4, steam sterilizing (temperature: 130 DEG C, pressure 0.17Mpa) 10 minutes, temperature dropped to 7 DEG C, adds compound gemma bacterial classification (subtilis: gel-shaped series bacillus: the mass ratio=1:1:1 of Bacillus licheniformis), constant air flow, steady temperature bottom fermentation 20 hours, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 90%, obtains degraded heavy metal composite microbial bacteria liquid.
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:4.The composite microbial bacteria liquid of degraded heavy metal and humic acid is made to mix packing bag fully for subsequent use.
(3) making of the microbial fertilizer of heavy metal is reduced: complex micro organism fungicide 15 parts, 11 parts, urea, monoammonium phosphate 18 parts, potassium sulfate 20 parts, sepiolite 20 parts, gac 20 parts, borax 2 parts, 2 parts, zinc sulfate, 5 parts, magnesium sulfate.Mix with stirrer, vertical crusher is pulverized, and carries out granulation by tablets press, finally dries, cools, the composite microbiological fertilizer of the reduction heavy metal of coating, packaging.
The microbial fertilizer reducing heavy metal described in embodiment 6 carries out field experiment
1. materials and methods
1.1 test sites: Chengwu County, Heze city of Shandong province economic and technological development zone
1.2 for examination soil
Experimental field soil fertility is medium.
1.3 for examination fertilizer
Microbial environment protecting fertilizer, common fertilizer.
1.4 for studying thing: garlic
1.5 test method
Adopt random district to arrange, establish 4 process altogether, repeat for four times, Ji15Ge community, plot area 20m
2.Process as follows:
Process 1: conventional fertilizer application+dispenser 2 times
Process 2: conventional fertilizer application+equivalent fine sand (200kg/667m
2)+dispenser 2 times
Process 3: conventional fertilizer application+complex microorganism environment-friendly fertilizer (200kg/667m
2)+dispenser 2 times
Process 4: conventional fertilizer application+complex microorganism environment-friendly fertilizer (200kg/667m
2)+dispenser 1 time
Farm manure 1000kg/667m is executed in each process respectively base
2, whole bottom application, other field management are identical with general production management.
1.6 cultivation management
Base manure, on October 1st, 2012, weighs up by test design requirement and applies each community, turn over by the interpolation proportioning test of different exogenous biological preparation respectively.On October 5th, 2012 is sowed.Sow first 7 days garlic clove is broken into two with one's hands, preserve garlic clove crust, remove moldy metamorphism and rocambole.945 lobes are sowed in each community.Planting density is distance between rows and hills 20cm.Other cultivation management measure is identical with production management.
On June 5th, 2013 head of garlic of gathering.By the single receipts in community, Dan Fang.Calculate output and carry out quality parameter mensuration.
By the process that the interpolation proportioning test of different exogenous biological preparation filters out, carry out different amounts test in October, 2012 to 2014 year, control measures are with the interpolation proportioning test of different exogenous biological preparation.
The Acquire and process of 1.7 samples
When garlic is gathered, record the output of each community.Randomly draw each community head of garlic, record single head of garlic weight, classification.After sample is gathered, respectively determination and analysis is carried out to fresh head of garlic garlicin, Vc content and heavy-metal residual.
1.8 index determining
1.8.1 test event and method
1.8.1.1 the mensuration ruler of garlic bolt plant height, the mensuration vernier callipers that stem is thick.Survey garlic bolt plant height on May 15th, 2013, stem is thick, random five strains measure: the mensuration of plant height is benchmark from rhizome portion to vegetative point, and stem is slightly being stem roughing standard under the first true leaf.
Table 1: different treatment is on garlic plant height, impact that stem is thick
As can be known from Table 1, process 1,2 is contrast, and compared with the control, the be significantly increased plant height of garlic, stem of other 2 process is thick, significant difference (p<0.05).And process 3,4 difference little, do not reach 0.05 level.
1.8.2 different treatment is on the impact of garlic shoot and head of garlic output
Table 2 different treatment is on the impact of garlic garlic shoot and head of garlic output
| Test process | Garlic shoot output (kg/667m 2） | Head of garlic output (kg/667m 2) |
| Process 1 | 595.01 ±5.57 | 895.21±6.11 |
| Process 2 | 601.22 ±9.24 | 908.02 ±7.45 |
| Process 3 | 788.74±5.13 | 1131.71 ±11.44 |
| Process 4 | 782.66±6.01 | 1141.70 ±10.98 |
As can be known from Table 2, process 3,4 can significantly improve the output of garlic, compared with the control, and each weight (p<0.05) processing be significantly increased garlic shoot and the head of garlic.
1.8.3 different treatment is on the impact of garlic classification
Process 3,4 firsts and seconds garlic proportions are all significant higher than process 1, and wherein one-level garlic improves 2.47,19.98,20.88 percentage points respectively, and secondary garlic improves 2,17.81,17.55 percentage points respectively.
1.8.4 on the impact of disease
Table 3 applies fertilizer on the impact of garlic leaf blight
| Test process | Investigation strain number (strain) | Morbidity strain number (strain) | Diseased plant rate (%) |
| Process 1 | 350 | 21 | 6.0 |
| Process 2 | 350 | 20 | 5.7 |
| Process 3 | 350 | 5 | 1.43 |
| Process 4 | 350 | 4 | 1.1 |
Test-results shows, fertilising enhances the resistance against diseases of garlic.In test, different fertilization leaf blight diseased plant rate is all low than contrast, and process 3,4 differences are extremely remarkable.Wherein minimum for 1.1-1.43% with the diseased plant rate processing 3 and 4.
1.8.5 heavy-metal residual detects
Table 4 heavy-metal residual detects
| Test item | Process 1 | Process 2 | Process 3 | Process 4 |
| Plumbous | 8 | 8 | 0 | 0 |
| Arsenic | 6 | 6 | 0 | 0 |
| Chromium | 0.25 | 0.28 | 0 | 0 |
| Mercury | 0.9 | 0.88 | 0.01 | 0.01 |
Select to use the fertilizer of above-described embodiment containing in the soil (this region is intermediate pollution region) of Ag, Cd heavy metal contamination near certain chemical plant in 2 kilometers, addition is 100kg/ mu, balanced soil improvement time 4 days.Through the soil of above-mentioned repair process, the shrub that it is planted grows fine, and grass seeds mulched ground indicated airspeed of reclaiming degree is fast.
Sampling analysis: heavy metal Ag, Cd in contaminated soil are reduced into immobilization heavy metal precipitation thing or mineral substance at a low price, lose its biological effectiveness, achieve the in-situ immobilization of heavy-metal contaminated soil, the soil environment quality after reparation meets the requirement of China " standard of soil environment quality " (GB15618-1995) primary standard value.
Claims (5)
1. reduce a microbial fertilizer for heavy metal, it is characterized in that: be made up of the raw material of following parts by weight:
Complex micro organism fungicide 15-25, urea 9-13, monoammonium phosphate 16-20, potassium sulfate 20-25, sepiolite 10-20, gac 10-20, borax 2-5, zinc sulfate 2-5, magnesium sulfate 3-5,
Wherein, complex micro organism fungicide is made primarily of the raw material of following weight: soybean cake powder 80-160kg/ ton, W-Gum 48-360kg/ ton, wheat bran 100-280kg/ ton, fish meal 30-80kg/ ton.
2. the preparation method of the microbial fertilizer of reduction heavy metal according to claim 1, is characterized in that: comprise the following steps:
(1) composite microbial bacteria liquid makes: by soybean cake powder, W-Gum, wheat bran, fish meal, soybean cake powder 80-160kg/ ton in proportion, W-Gum 48-360kg/ ton, wheat bran 100-280kg/ ton, fish meal 30-80kg/ ton, to add in fermentor tank and to inject corresponding water, pH value is adjusted to 7.2-7.8, steam sterilizing, temperature drops to 7-35 DEG C, adds compound gemma bacterial classification, at constant air flow, steady temperature bottom fermentation, one-tenth spore rate through sediments microscope inspection genus bacillus reaches 85-90%, obtains degraded heavy metal composite microbial bacteria liquid;
(2) making of complex micro organism fungicide: composite microbial bacteria liquid and humic acid are carried out mixing and absorption according to the ratio of 1:3-1:5, makes the composite microbial bacteria liquid of degraded heavy metal and humic acid mix packing bag fully for subsequent use;
(3) making of the microbial fertilizer of heavy metal is reduced: by complex micro organism fungicide 15-25 part; urea 9-13 part, monoammonium phosphate 16-20 part, potassium sulfate 20-25 part, sepiolite 10-20 part; gac 10-20 part; borax 2-5 part, zinc sulfate 2-5 part, magnesium sulfate 3-5 part; mix with stirrer; vertical crusher is pulverized, and carries out granulation, finally dries, cools, coating, packs reduce the composite microbiological fertilizer of heavy metal by tablets press.
3. the preparation method of the microbial fertilizer of reduction heavy metal according to claim 2, is characterized in that: in step (1), and steam sterilizing temperature is 115-130 DEG C, pressure 0.07-0.17Mpa.
4. the preparation method of the microbial fertilizer of reduction heavy metal according to claim 3, is characterized in that: in step (1), and sterilization time is 10-60min.
5. the preparation method of the microbial fertilizer of the reduction heavy metal according to Claims 2 or 3 or 4, is characterized in that: in step (1), and fermentation time is 16-20 hour.
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Application publication date: 20151230 |