CN102795934B - Method and device for manufacturing microbial compound fertilizers - Google Patents
Method and device for manufacturing microbial compound fertilizers Download PDFInfo
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- CN102795934B CN102795934B CN201210313134.XA CN201210313134A CN102795934B CN 102795934 B CN102795934 B CN 102795934B CN 201210313134 A CN201210313134 A CN 201210313134A CN 102795934 B CN102795934 B CN 102795934B
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Abstract
The invention provides a method for manufacturing microbial compound fertilizers, which comprises the steps of 1) cultivating the original strain to obtain compound microbial inoculum and mixing the compound microbial inoculum, a nutrient component and a strain carrier uniformly in proportion to prepare slurry, 2) mixing the rest raw materials in proportion, granulating, drying, cooling and then grading to obtain a compound granulated fertilizer, and 3) sprinkling the slurry into the compound granulated fertilizer for inoculation, drying at a low temperature, cooling, measuring and packaging to obtain the microbial compound fertilizer. The invention further provides a device for implementing the manufacturing method of the microbial compound fertilizer, which is composed of a compound granulation device and a slurrying inoculation device that are connected with each other by a conveyer belt. The manufacturing method and the device have the advantages that the microbial compound fertilizer produced by the method is high in content of total effective viable bacteria, high in strain activity, long in fertilizer efficiency period and good in economic benefit, has a function of a slow-release fertilizer, and can improve the soil and environment.
Description
Technical field
The present invention relates to microbial fertilizer preparation, specifically a kind of microbiological compound fertilizer material manufacture method and device thereof.
Background technology
In recent years, because the unreasonable of chemical fertilizer, agricultural chemicals used, expend a large amount of non-renewable resources, and destroyed Soil structure, polluted environment, reduced quality of agricultural product, affected the mankind's health existence.
And microbial fertilizer is as the important source of manure of another agriculture production outside chemical fertilizer, organic fertilizer, there is following advantage: 1) can provide crop alimentary, inorganic elements can be converted into the organic compound of useful plants growth, in raising air, the utilising efficiency of nutrients resource in nitrogen or soil, has reduced production cost; 2) lower nitrogen deoxidation and oxidising process, reduce nitrate content, thereby improve crop quality, raising crop yield; 3) can improve redox of soil condition, decompose the pollutent of eliminating in soil, improve to a certain extent the physics and chemistry of soil, play the effect of soil remediation, reduce environmental pollution.Therefore,, from the development trend of green agriculture, the ecological agriculture, non-pollution microbial fertilizer material free from environmental pollution will play a significant role in the future of agriculture is produced.Existing multiple countries production in the world, using microbe fertilizer, Chinese Ye You 300 many enterprises carry out microbial fertilizer production, the average annual about few hundred thousand tonnes of of output, the area of its application has exceeded 1,670,000 sq-kms.
The existing microbial fertilizer of China is basis mainly with organic matter greatly, is equipped with microbial inoculum and inorganic fertilizer, mixes.A kind of good microbial fertilizer has strict requirement at aspects such as living bacteria count, water content, pH value, absorbent particles fineness, organic content, miscellaneous bacteria rate and shelf lifes, wherein, the beneficial microorganism quantity containing and the vigorous degree of vital movement are the keys of microbial fertilizer quality, are also the key factors that affects its effect.
Research and development, the quality product of Chinese Government to microbial fertilizer, apply and pay much attention to, carry out production permit system and also audit and provide by the Ministry of Agriculture.Along with the development of microbial fertilizer, few places has occurred falling behind, use the phenomenons such as the bacterial classification not being tested and appraised is produced, effectively bacterial content is low, miscellaneous bacteria exceeds standard without production licence, production technique, also some producer adds chemical fertilizer or other additive of underexercise in microbial fertilizer, make that nitrogen, phosphorus, the potassium content of chemical fertilizer in fertilizer is too high causes effective bacterium death, and lose the effect of microbial fertilizer.
Summary of the invention
The object of the invention is to the deficiencies such as in producing for microbial fertilizer, the technique of existence is backward, the effective bacterial content of product is low, miscellaneous bacteria exceeds standard, a kind of microbiological compound fertilizer material manufacture method and device thereof are provided.
Microbiological compound fertilizer material manufacture method provided by the invention, concrete steps are as follows:
1) cultivate original strain and obtain composite fungus agent, composite fungus agent, nutritive ingredient, bacterial classification carrier are mixed in the ratio of 1:5:100, add water and make slip;
2) mix all the other raw materials in microbiological compound fertilizer material formula ratio, granulation, classification after Drying and cooling, obtains compound granulated fertilizer;
3) slip step 1) being made is sprayed at step 2) to inoculate in described compound granulated fertilizer, oven drying at low temperature, cooling, metering packing, obtain microbiological compound fertilizer material.
Wherein, cultivate original strain described in step 1) and obtain composite fungus agent, comprise the steps:
A prepares bacterium and mixes spore powder: each primitive bacteria bacterial classification is carried out successively respectively under aseptic condition, after slant culture, shaking table cultivation, fermentor cultivation, the fermented liquid obtaining is mixed by formula rate, be prepared into bacterium mix spore powder through concentrate drying;
B prepares fungal spore powder: original fungi strain is carried out successively under aseptic condition after slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore, produced the substratum of spore completely, be immersed in clear water and make fungal spore suspension, concentrate drying is prepared into fungal spore powder;
The bacterium that c makes step a, b mixes spore powder and fungal spore powder mixes and obtains composite fungus agent by formula rate.
Wherein, the nutritive ingredient described in step 1) is prepared by the following method: mix in the ratio of 1:2.8:5:1:0.2 by white sugar, brown sugar, starch, protein powder, with the mineral element of amino acid and/or humic acid chelating, make nutritive ingredient; Described mineral element is iron, boron, zinc, copper, manganese and molybdenum.
Wherein, bacterial classification carrier is wilkinite, sepiolite and humic acids described in step 1), pulverizes respectively, crosses 200 mesh sieves, mixes and makes in the ratio of 1:1:2.
For different microbiological compound fertilizer material products, bacterial classification kind, the quantity of composite fungus agent used while preparing slip, and the raw material such as bacterial classification carrier and nutritive ingredient can be done corresponding adjustment by its formula.
Wherein, the temperature of slip is controlled at 35-40 DEG C described in step 1), and water content is controlled at 25-30%.
Wherein, step 2) described granulation, the uniform particles that requirement is prepared, Specifications meets national standard or Ministry of Agriculture's industry standard.
Wherein, spray described in step 3), control its flow, to guarantee that living bacteria count, total nutrient and organic content in finished product microbiological compound fertilizer material is equal to or greater than relevant criterion and is as the criterion.
Wherein, oven drying at low temperature described in step 3), temperature is 55-60 DEG C, wind-force is 8.0-10.7m/s.
The present invention also provides the microbiological compound fertilizer material manufacturing installation of application mentioned microorganism composite fertilizer manufacture method, is made up of compound prilling granulator and slurrying classification inoculation apparatus, and both connect with conveying belt.
Compound prilling granulator is mainly made up of stirrer, tablets press, dryer and cooler, is connected successively with conveying belt.
Wherein, before stirrer, also can establish pulverizer and sieving machine, after cooler, also can establish sizing screen, be connected with conveying belt.
Slurrying classification inoculation apparatus is mainly made up of pulping tank, slurry can, spray pump, cylinder vortex mixer, oven drying at low temperature equipment, slip is put to slurry can by the bleed valve of pulping tank bottom, slurry can bottom connects spray pump, the spout of spray pump is suspended in the top in cylinder vortex mixer, cylinder vortex mixer upstream is connected with compound prilling granulator by conveying belt, and downstream connects oven drying at low temperature equipment by conveying belt.
Wherein, after oven drying at low temperature equipment, also can connect cooler, wrapping machine.
Wherein, in the tank of pulping tank, slurry can, be respectively equipped with agitator, outer wall is equipped with chuck, and outer wall materials is corrosion resistant material, preferably enamel.
Wherein, pulping tank bottom drain valve is just provided with filter screen, to avoid the caking in slip to enter slurry can.
Wherein, spray pump spray pressure can regulate voluntarily, is preferably vertically contained in cylinder vortex mixer top, its two equidistant opening of difference in below several.
The working process of said apparatus is as follows:
Pack in proportion composite fungus agent, nutritive ingredient, bacterial classification carrier into pulping tank, in pulping tank outer wall chuck, pass into steam and heat, temperature in pulping tank is controlled to 35-40 ° of C, add water and make slip; Open the bleed valve of pulping tank bottom, the slip making is put to slurry can, in slurry can outer wall chuck, also pass into steam and heat, temperature in slurry can is controlled to 35-40 ° of C.
Simultaneously; packing all the other raw materials into pulverizer in microbiological compound fertilizer material formula ratio pulverizes; raw material after pulverizing is transported to stirrer, tablets press, dryer, cooler and sizing screen successively through conveying belt; successively mix, granulation, oven dry, cooling, classification; obtain compound granulated fertilizer, be transported in cylinder vortex mixer.
The spray pump that is connected to slurry can bottom is sprayed onto slip on the compound granulated fertilizer in cylinder vortex mixer, mix inoculation, deliver to successively oven drying at low temperature equipment (temperature 55-60 DEG C by conveying belt again, wind-force 8.0-10.7m/s) and cooling system carry out oven drying at low temperature and cooling, finally detect and metering packing, obtain microbiological compound fertilizer material.
The advantage of microbiological compound fertilizer material manufacture method provided by the invention is as follows:
1, in traditional microbiological compound fertilizer material manufacturing processed, generally after bacterial classification inoculation, carry out granulating and forming, oven dry, cause the even mortality of activity decreased of microbial strains, after seeded process has been placed on granulating and forming by the present invention, avoided to a great extent the forfeiture of microbial strains activity.
2, strictly to control the temperature of slip be 35-40 DEG C in the present invention, and water content is 25-30%, is suitable for microbial strains growth and breeding, guarantees microorganism active.The product that adopts the present invention to produce, total living bacteria count content is large, and bacterial activity is high.
3, bacterial classification carrier of the present invention is wilkinite, sepiolite and humic acids, and complexing ammonium ion, phosphate anion and potassium ion etc. preferably prevent that the leaching of available nutrient runs off, and improve utilization rate of fertilizer, extend fertilizer efficiency period.
4, beneficial microorganism bacterial classification and bacterial classification carrier and nutritive ingredient are made slip by the present invention, be sprayed on the compound granulated fertilizer of granulating and forming and inoculate, self-assembling formation the coating layer of fertiliser granulates, have slow (control) concurrently and release fertile function.
5, in traditional microbiological compound fertilizer material manufacturing processed, conventionally adopt hyperthermia drying, it is even dead that this can make microorganism active reduce, the present invention adopts the mode of oven drying at low temperature, temperature should be controlled at 55-60 DEG C, wind control is at 8.0-10.7m/s, can complete drying course, and avoid the microorganism deactivated and death under hot environment.
6, adopt the present invention can produce all kinds, various ratio and total effectively bacterium number, total nutrient content, microbiological compound fertilizer material that organic content is different, as long as simple bacterial classification kind, the quantity of adjusting composite fungus agent used, and the ratio of bacterial classification carrier, nutritive ingredient and other raw material.
7, processing condition of the present invention are more gentle, easy to implement.
Brief description of the drawings
Fig. 1 is the slurrying classification inoculation apparatus structural representation of microbiological compound fertilizer material manufacturing installation of the present invention.
Wherein, 1: gauger, 2: stirrer, 3: bucket elevator, 4: pulping tank, 5: slurry can, 6: spray pump, 7: cylinder vortex mixer, 8: oven drying at low temperature equipment, 9: cooling system.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 microbiological compound fertilizer material of the present invention manufacturing installation
The device that is applied to the manufacture of microbiological compound fertilizer material is made up of compound prilling granulator and slurrying classification inoculation apparatus (as shown in Figure 1), and both connect with conveying belt.
Pulping raw material stirs after taking with gauger 1 in stirrer 2, with bucket elevator 3, batching is sent into the pulping tank 4 of slurrying classification inoculation apparatus.
Slurrying classification inoculation apparatus is made up of pulping tank 4, slurry can 5, spray pump 6, cylinder vortex mixer 7, oven drying at low temperature equipment 8 and cooling system 9, slip is put to slurry can 5 by the bleed valve of pulping tank 4 bottoms, slurry can 5 bottoms connect spray pump 6, the top of the spout of spray pump 6 in cylinder vortex mixer 7, cylinder vortex mixer 7 upstreams are connected with compound prilling granulator by conveying belt, and downstream connects oven drying at low temperature equipment 8 and cooling system 9 successively by conveying belt.
In the tank of pulping tank 4, slurry can 5, be respectively equipped with agitator, outer wall is equipped with chuck, can be filled with steam, and outer wall materials is enamel.Pulping tank 4 bottom drain valve are just provided with filter screen, to avoid the caking in slip to enter slurry can.
Spray pump 5 spray pressures can regulate voluntarily, are vertically contained in the top in cylinder vortex mixer 7, and long 1.8 meters, 17 of its two of belows equidistant openings of difference, 3 millimeters of bores.
Compound prilling granulator comprises pulverizer, sieving machine, stirrer, tablets press, dryer, cooler and sizing screen, is connected successively with conveying belt, and downstream connects cylinder vortex mixer 7.
Embodiment 2 produces microbiological compound fertilizer material according to method of the present invention
Bacillus licheniformis, bacillus laterosporus, bacillus pumilus, bacillus megaterium, silicate bacteria are cultivated into respectively to spore powder, proportionally mix spore powder and obtain composite fungus agent, packing composite fungus agent, nutritive ingredient, bacterial classification carrier into pulping tank in the ratio of 1:5:100 adds water, making mixtures of materials water content is 25-30%, in pulping tank outer wall chuck, passing into steam heats, temperature in pulping tank is controlled to 35-40 DEG C, stirs evenly material with agitator and make slip; Open the bleed valve of pulping tank bottom, the slip making is put to slurry can through filter screen, also passes into steam and heat in slurry can outer wall chuck, and temperature in slurry can is controlled to 35-40 DEG C, stirs slip with agitator.
Meanwhile, take ammonium nitrate 8.4kg, monoammonium phosphate 7kg, diammonium phosphate 16.5kg, potassium sulfate 10kg, saltpetre 11kg, Repone K 2kg, borax 1kg, zinc sulfate 1kg, magnesium sulfate 1kg.Separately add more than 65% humic acids 10kg, the sepiolite 32kg of organic content.Nitrogen, phosphorus, the potassium ratio controlled are wherein 8:11:11, and total nutrient is more than or equal to 30%.Pack above-mentioned raw materials into stirrer, add water (it is agglomerating that amount of water control is that material hand is pinched, and lands loose) mixes; transport to tablets press, dryer and cooler by travelling belt successively; successively carry out granulation, oven dry, cooling, obtain compound granulated fertilizer, be transported in cylinder vortex mixer.
Spray pump is sprayed onto slip on the compound granulated fertilizer in cylinder vortex mixer, mix inoculation, control slip flow, make bacterial classification viable count in product reach respectively following technical indicator: Bacillus licheniformis 100,000,000 cfu/g, bacillus laterosporus 0.5 hundred million cfu/g, bacillus pumilus 0.5 hundred million cfu/g, bacillus megaterium 200,000,000 cfu/g, silicate bacteria 100,000,000 cfu/g, total living bacteria count >=500,000,000 cfu/g.
Compound granulated fertilizer after inoculation is delivered to oven drying at low temperature equipment (temperature 55-60 DEG C, wind-force 8.0-10.7m/s) successively by conveying belt, and cooling rear detection metering packing, obtain microbiological compound fertilizer material.
After testing, total living bacteria count content >=500,000,000 cfu/g of this microbiological compound fertilizer material, organic >=5%, total nutrient >=30%.
Embodiment 3 produces microbiological compound fertilizer material according to method of the present invention
Bacillus licheniformis, bacillus laterosporus, bacillus pumilus, bacillus megaterium, silicate bacteria are cultivated into respectively to spore powder, proportionally mix spore powder and obtain composite fungus agent, packing composite fungus agent, nutritive ingredient, bacterial classification carrier into pulping tank in the ratio of 1:5:100 adds water, making mixtures of materials water content is 25-30%, in pulping tank outer wall chuck, passing into steam heats, temperature in pulping tank is controlled to 35-40 DEG C, stirs evenly material with agitator and make slip; Open the bleed valve of pulping tank bottom, the slip making is put to slurry can through filter screen, also passes into steam and heat in slurry can outer wall chuck, and temperature in slurry can is controlled to 35-40 DEG C, stirs slip with agitator.
Meanwhile, take urea 12.5kg, monoammonium phosphate 11.5kg, calcium superphosphate 0.5kg, ammonium chloride 20kg, Repone K 13.5kg, borax 0.5kg, zinc sulfate 0.5kg.Separately add more than 65% humic acids 20kg, the more than 40% agricultural crop straw 21kg becoming thoroughly decomposed of organic content of organic content, nitrogen wherein, phosphorus, potassium ratio are 12:5:8, and total nutrient is more than or equal to 25%, and organic matter is more than or equal to 20%.
Pack above-mentioned raw materials into stirrer, add water (it is agglomerating that amount of water control is that material hand is pinched, and lands loose) mixes; transport to tablets press, dryer and cooler by travelling belt successively; successively carry out granulation, oven dry, cooling, obtain compound granulated fertilizer, be transported in cylinder vortex mixer.
Spray pump is sprayed onto slip on the compound granulated fertilizer in cylinder vortex mixer, mix inoculation, control slip flow, make bacterial classification viable count in product reach respectively following technical indicator: Bacillus licheniformis 100,000,000 cfu/g, bacillus laterosporus 0.5 hundred million cfu/g, bacillus pumilus 0.5 hundred million cfu/g, bacillus megaterium 200,000,000 cfu/g, silicate bacteria 100,000,000 cfu/g, total living bacteria count >=500,000,000 cfu/g
Compound granulated fertilizer after inoculation is delivered to oven drying at low temperature equipment (temperature 55-60 DEG C, wind-force 8.0-10.7m/s) successively by conveying belt, and cooling rear detection metering packing, obtain microbiological compound fertilizer material.
After testing, total living bacteria count content >=500,000,000 cfu/g of this microbiological compound fertilizer material, organic >=20%, total nutrient >=25%.
Embodiment 4 produces microbiological compound fertilizer material according to method of the present invention
Bacillus licheniformis, bacillus laterosporus, bacillus pumilus, bacillus megaterium, silicate bacteria are cultivated into respectively to spore powder, proportionally mix spore powder and obtain composite fungus agent, packing composite fungus agent, nutritive ingredient, bacterial classification carrier into pulping tank in the ratio of 1:5:100 adds water, making mixtures of materials water content is 25-30%, in pulping tank outer wall chuck, pass into steam and heat, temperature in pulping tank is controlled to 35-40 DEG C and stirs evenly material with agitator and make slip; Open the bleed valve of pulping tank bottom, the slip making is put to slurry can through filter screen, also passes into steam and heat in slurry can outer wall chuck, and temperature in slurry can is controlled to 35-40 DEG C, stirs slip with agitator.
Simultaneously, take more than 65% humic acids 50kg, the more than 40% agricultural crop straw 15kg becoming thoroughly decomposed, sepiolite 10kg, the wilkinite 15kg of organic content of monoammonium phosphate 10kg, organic content, nitrogen wherein, phosphorus, potassium total nutrient are more than or equal to 8%, and organic matter is more than or equal to 40%.
Pack above-mentioned raw materials into stirrer, add water (amount of water standard: material hand is pinched agglomerating is landed loose) mixes; transport to tablets press, dryer and cooler by travelling belt successively; successively carry out granulation, oven dry, cooling, obtain compound granulated fertilizer, be transported in cylinder vortex mixer.
Spray pump is sprayed onto slip on the compound granulated fertilizer in cylinder vortex mixer, mix inoculation, control slip flow, make bacterial classification viable count in product reach respectively following technical indicator: Bacillus licheniformis 100,000,000 cfu/g, bacillus laterosporus 0.5 hundred million cfu/g, bacillus pumilus 0.5 hundred million cfu/g, bacillus megaterium 200,000,000 cfu/g, silicate bacteria 100,000,000 cfu/g, total living bacteria count >=500,000,000 cfu/g.
Compound granulated fertilizer after inoculation is delivered to oven drying at low temperature equipment (temperature 55-60 DEG C, wind-force 8.0-10.7m/s) successively by conveying belt, and cooling rear detection metering packing, obtain microbiological compound fertilizer material.
After testing, total living bacteria count content >=500,000,000 of this microbiological compound fertilizer material/gram, organic >=40%, total nutrient >=8%.
The microbiological compound fertilizer material application effect that embodiment 5 produces according to method of the present invention
For verifying the effect of the microbiological compound fertilizer material that method of the present invention produces, in the farmland covered by contract of Soviet Union of hair members of a family village, hair town, Jianli County fame and prestige, plant rape variety Hunan No. 188 (seed is purchased from Hunan Ya Huazhongye grain and oil seeds company) of assorted oil, carry out following processing:
Test site arranges 4 processing, repeats for 3 times, and Gong12Ge community, random district group is arranged, and community is rectangle, area 33.3m
2.Previous crops late rice, rape was sowed on September 20th, 2009, and October 22, individual plant was transplanted, and on April 30th, 2010 gathers in the crops.Seeding row spacing is 50cm × 25cm, and experimental field surrounding protection row 1.5cm is wide.The microbiological compound fertilizer material formula that method of the present invention is produced and production method are referring to embodiment 4.
Process 1: farmyard manure 15000 kg/ha are done base manure row replacement;
Process 2: microbiological compound fertilizer material 1500 kg/ha that method of the present invention is produced are done base manure row replacement;
Process 3: microbiological compound fertilizer material 1500 kg/ha that the method for the present invention after deactivation is produced are done base manure row replacement;
Process 4: do base manure row replacement by thin sand and soil 15000 kg/ha.
The fertile fertilizing standards of physics and chemistry is N165 kg/ha, P everywhere
2o
560 kg/ha, K
2o 90 kg/ha, borax 15 kg/ha, fertilizer used is purchased from the 46% Dongting Lake board urea purchased from Jianli County, Hubei Province means of agricultural production service centre of bureau of agriculture, 60 Canadian Repone K, 12% clear river board peracid calcium phosphorus, and other management are produced consistent with general rape field.
December 22, randomly draw each processing 10 strain plant, measure the length of maximum leaf and wide, the maximum leaf mean length of rape of using the microbiological compound fertilizer material processing of method production of the present invention is 34.2cm, width is 13.5cm, respectively than processing 1, process 3, processing 4 the long 2.6cm of maximum leaf length, 3.3cm, 4.8cm, the long 0.4cm of maximum width of blade degree, 0.5cm, 0.8cm, and the green matter of the large look of leaf of processing 2 is thick, and growth potential is strong.
Before harvesting, sampling species test result shows, use the rape plant height 168cm of the microbiological compound fertilizer material of method production of the present invention, compared with the high 9-20cm of other processing, once effective 6.9 of branches of individual plant, process many 0.4-1.1 compared with other, its individual plant angle fruit number, seed number per pod equal yield line factor structure are all better than other and process (in table 1).
The different base manure of table 1 is learned the impact of proterties on brassinosteroid biosynthesis
The impact of the different base manure of table 2 on yield of rape
The actual output of processing 2, higher than processing 3, is processed 1 and is reached conspicuous level, processes 4 and reaches utmost point conspicuous level (in table 2).
From cost-effectiveness comparison (not considering improving soil benefit), Semen Brassicae campestris is by 4.2 yuan/kilogram of market purchase average prices, 1000 yuan/ton of meters of microbiological compound fertilizer material that 100 yuan/ton of farmyard manures, method of the present invention are produced, in this test, use the rape of the microbiological compound fertilizer material of method production of the present invention, realized increasing both production and income (in table 3).
The rape economic benefits comparison of the different base manure processing of table 3
Separately in rape breeding time, virus disease, the oidium to different treatment is carried out respectively investigation records, investigation result shows, the sickness rate of processing 2 Rape virus diseases has reduced by 35% than processing 4, and the sickness rate of oidium has reduced by 58% than processing 4, all reaches utmost point conspicuous level.
Above-mentioned test-results shows, adopts the microbiological compound fertilizer material that method of the present invention is produced to have obvious promoter action and significant effect of increasing production to growing of rape.This microbiological compound fertilizer material is preserved a large amount of useful active bacterias, after being manured into soil, can near plant rhizosphere, surely grow and breeding rapidly, microbiotic effective distribution and the environment of an activation are improved, improved ecological environment of soil, promoted that Root Absorption utilizes nutrient, the movable derivative of fungus strain can also directly stimulate healthy growth of crops simultaneously, thereby reach the object that improves crop anti-adversity and product quality, therefore, there is good production application and be worth, be worth spread.
The microbiological compound fertilizer material application effect that embodiment 6 produces according to method of the present invention
For verifying the effect of the microbiological compound fertilizer material that method of the present invention produces, in the farmland covered by contract of the merit that continues, plant Hunan No. 10 (seed is purchased from Hunan Ya Huazhongye grain and oil seeds company) of oil of rape variety China in Bai Sheng village, Meng Xi town, Lixian County, carry out following processing:
Test site arranges 3 processing, repeats for 3 times, and totally 9 communities, random district group is arranged, and community is rectangle, area 33.3m
2.Previous crops late rice, rape was sowed on September 11st, 2009, and October 15, individual plant was transplanted, and on March 18th, 2010 gathers in the crops, planting density 112500 strain/hectares, experimental field surrounding protection row 1.5cm is wide.Common micro-organisms composite fertilizer is as a comparison selected commercial Jie garden board biological organic fertilizer, and Jie Yuan Environmental Protection Technology Co., Ltd of Henan Province produces.The microbiological compound fertilizer material formula that method of the present invention is produced and production method are referring to embodiment 4.
Process 1: farmyard manure 15000 kg/ha are done base manure row replacement;
Process 2: common micro-organisms composite fertilizer 1500 kg/ha are done base manure row replacement;
Process 3: microbiological compound fertilizer material 1500 kg/ha that method of the present invention is produced are done base manure row replacement.
Each field management measure of processing is consistent, and flower bud a kind of sedge phase imposes 25 kgs/acre of composite fertilizers, and Seedling Stage, flower bud a kind of sedge phase respectively spray 0.2% boron fertilizer once, and full-bloom stage sprays 1000 times of liquid of 80% derosal and prevents and treats sclerotium disease, with apply fertilizer and agricultural chemicals purchased from Agricultural Materials Company.When investigation, average by five point samplings in every community; When results, the single receipts in each community, singles, list shine, calculate separately output.
The economic characters such as the rape plant height through different treatment, effective branch amount, thousand seed weight are investigated and record, be the results are shown in Table 4.Processing 3 contrasts and compares with other, economic characters are greatly improved, compared with using the processing 2 of common micro-organisms composite fertilizer, the effective branch amount of plant height, individual plant of processing 3, individual plant effective angle fruit number, every seed number per pod and thousand seed weight have increased respectively 1cm, 0.4,7.2,1.3 and 0.2g; Compared with using the processing 1 of farmyard manure, the effective branch amount of plant height, individual plant of processing 3, individual plant effective angle fruit number, every seed number per pod and thousand seed weight have increased respectively 3cm, 1.1,25.7,1.7 and 0.3g.As can be seen here, use microbiological compound fertilizer material that method of the present invention produces and can well improve the economic characters of rape.
The different base manure of table 4 is learned the impact of proterties on brassinosteroid biosynthesis
To adding up through different treatment Ge community yield of rape, the results are shown in Table 5.Use the microbiological compound fertilizer material (processing 3) of method production of the present invention than using farmyard manure (processing 1) volume increase 420 kg/ha, stimulation ratio is 16.1%; Than using common micro-organisms composite fertilizer (processing 2) volume increase 300 kg/ha, stimulation ratio reaches 10.9%.Significance of difference analysis by table 5, table 6 is known, and the microbiological compound fertilizer material of using method production of the present invention can promote yield of Brassica napus L, and effect of increasing production is extremely remarkable.
The impact of the different base manure of table 5 on yield of rape
Table 6 analysis of variance table
The table 7 different treatment cell production significance of difference is analyzed (LSD method)
From cost-effectiveness comparison (not considering improving soil benefit), Semen Brassicae campestris is by 4.2 yuan/kilogram of market purchase average prices, 1000 yuan/ton, the microbiological compound fertilizer material that 100 yuan/ton of farmyard manures, method of the present invention are produced, 1200 yuan/ton of (purchasing in fact valency) meters of common micro-organisms composite fertilizer, in this test, the rape of using the microbiological compound fertilizer material of method production of the present invention increases net return than the processing 1 of using farmyard manure increases by 1764.0 yuan/hectare, increase by 1560.0 yuan/hectare of net return than the processing 2 of using common micro-organisms composite fertilizer, concrete outcome is in table 8.
The rape economic benefits comparison of the different base manure processing of table 8
The above results shows, the microbiological compound fertilizer material that employing the present invention produces is compared with traditional farmyard manure and common micro-organisms composite fertilizer, can not only improve the economic characters of rape, strengthen the anti-adversity abilities such as rape is resistant to lodging, disease and insect resistance, and can promote yield of Brassica napus L high-quality, reach the object of increasing both production and income, on producing, there is good effect.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (1)
1. a microbiological compound fertilizer material manufacture method, concrete steps are as follows:
1) cultivate original strain and obtain composite fungus agent, composite fungus agent, nutritive ingredient, bacterial classification carrier are mixed in the ratio of 1:5:100, add water and make slip;
2) mix all the other raw materials in microbiological compound fertilizer material formula ratio, granulation, classification after Drying and cooling, obtains compound granulated fertilizer;
3) by step 1) slip that makes is sprayed at step 2) inoculate in described compound granulated fertilizer, oven drying at low temperature, cooling, metering packing, obtain microbiological compound fertilizer material;
Step 1) described original strain is Bacillus licheniformis, bacillus laterosporus, bacillus pumilus, bacillus megaterium and silicate bacteria;
Step 1) described cultivation original strain obtains composite fungus agent, comprises the steps:
A prepares bacterium and mixes spore powder: each primitive bacteria bacterial classification is carried out successively respectively under aseptic condition, after slant culture, shaking table cultivation, fermentor cultivation, the fermented liquid obtaining is mixed by formula rate, be prepared into bacterium mix spore powder through concentrate drying;
B prepares fungal spore powder: original fungi strain is carried out successively under aseptic condition after slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore, produced the substratum of spore completely, be immersed in clear water and make fungal spore suspension, concentrate drying is prepared into fungal spore powder;
The bacterium that c makes step a, b mixes spore powder and fungal spore powder mixes and obtains composite fungus agent by formula rate;
Step 1) described nutritive ingredient prepared by the following method: mixes in the ratio of 1:2.8:5:1:0.2 by white sugar, brown sugar, starch, protein powder, with the mineral element of amino acid and/or humic acid chelating, makes nutritive ingredient; Described mineral element is iron, boron, zinc, copper, manganese and molybdenum;
Step 1) described bacterial classification carrier is wilkinite, sepiolite and humic acids, pulverizes respectively, crosses 200 mesh sieves, mix and make in the ratio of 1:1:2;
Step 1) temperature of described slip is controlled at 35-40 DEG C, and water content is controlled at 25-30%;
Step 3) described oven drying at low temperature, temperature is 55-60 DEG C, wind-force is 8.0-10.7m/s.
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| CN103819267B (en) * | 2014-02-07 | 2016-01-06 | 湖南泰谷生物科技股份有限公司 | For the microbial-bacterial fertilizer and preparation method thereof of peanut cultivation |
| CN105061085B (en) * | 2015-08-22 | 2018-07-17 | 山东金利丰生物科技股份有限公司 | A kind of biology coating organic-inorganic microbial manure and preparation method thereof |
| CN105409709B (en) * | 2015-11-25 | 2018-10-16 | 遂宁市夏曾种植有限公司 | A kind of implantation methods of red grape |
| CN106007820B (en) * | 2016-07-25 | 2019-04-05 | 上海化工研究院有限公司 | Device and the application of active bacteria are added in a kind of material of granular organic fertilizer after the drying |
| CN106905050A (en) * | 2017-03-25 | 2017-06-30 | 山东福龙生物化肥有限公司 | A kind of feature fruit tree special bio microbial inoculum |
| CN110305776B (en) * | 2019-07-10 | 2024-02-06 | 何水桥 | Preparation facilities is used to ecological remediation fungus agent based on pond quality of water improves |
| CN110423169A (en) * | 2019-08-28 | 2019-11-08 | 孙国萍 | A kind of composite fertilizer |
| CN110423159A (en) * | 2019-08-28 | 2019-11-08 | 孙国萍 | A kind of production method of complex fertilizer |
| CN112592222A (en) * | 2020-11-05 | 2021-04-02 | 东北农业大学 | Solid-state composite microorganism rooting agent for improving saline-alkali soil and application thereof |
| CN114854422B (en) * | 2022-03-14 | 2023-05-23 | 河北工程大学 | Saline-alkali soil conditioner rich in salt-tolerant microorganisms and preparation method and device thereof |
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