CN103461394B - Air refreshing disinfectant - Google Patents
Air refreshing disinfectant Download PDFInfo
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- CN103461394B CN103461394B CN201310283575.4A CN201310283575A CN103461394B CN 103461394 B CN103461394 B CN 103461394B CN 201310283575 A CN201310283575 A CN 201310283575A CN 103461394 B CN103461394 B CN 103461394B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
Most of the air refreshing disinfectants sold on the market comprise synthetic chemical sterilizing agents, and harm on human body may be caused by usual exposure in the air containing the chemical products. Therefore the invention provides an air refreshing disinfectant, and especially an air refreshing disinfectant, in which non-synthetic natural materials are taken as raw materials, and traditional Chinese medicine type volatile oils are taken as major active ingredients. The air refreshing disinfectant is capable of inhibiting bacteria, and virus, and is beneficial for human health; and fragrance of the air refreshing disinfectant lasts long. The traditional Chinese medicine type volatile oils are capable of inhibiting bacteria, and virus, and possess fragrance. The above characteristics of the traditional Chinese medicine type volatile oils are used in the air refreshing disinfectant. A composition of eucalyptus oil, clove oil, patchouli oil, forsythia suspansa oil and schizonepeta oil is taken as the active ingredient of the air refreshing disinfectant.
Description
Technical field
The present invention relates to a kind of air disinfectant, particularly a kind of with what non-chemically synthesize, natural material is the volatile oil of the Traditional-Chinese-medicine-type of raw material is the air disinfectant of principle active component.
Technical background
Environment goes from bad to worse, and in air, harmful substance increases, and peculiar smell, air infection affect mood, health, the operating efficiency of people.Cold, influenza or the infection of the upper respiratory tract are common disease and the frequently-occurring disease of winter-spring season, and it is propagated and infects mainly through the spittle and air cross, and especially in the place that the crowd is dense, air not easily circulates, as hospital, school, company etc., incidence is very high.Mainly owing to there is more pathogenic microorganism in this, as influenza virus, rhinovirus, Respiratory Syncytial Virus(RSV), adenovirus, hemolytic streptococcus, staphylococcus, Escherichia coli etc. in the air of place.Therefore, carrying out disinfection to air is one of the important measures of this cross-infection of prevention and corntrol.Remove peculiar smell in order to the harmful microorganism killed in air, Modern Family, hospital, hotel, restaurant and some other public arena, often use air disinfectant in air.But the air disinfectant sold in the market, the chemosterilant mostly containing Prof. Du Yucang, is often exposed to this containing in the air of chemicals, may knows from experience cause certain injury to people.Therefore, for purifying the air of a room, developing the pure and fresh sterile products of natural air safely and effectively, seeming particularly important.
Summary of the invention
The present invention is directed to the technical deficiency of existing with fresh air and disinfectant etc., disclose a kind of air disinfectant being principle active component with the volatile oil of Traditional-Chinese-medicine-type, there is antibacterial, antiviral, fragrant lasting, economic and practical feature, be of value to the health of human body.
In order to the obvious prescription of screening effect, have selected the evaluation that the antibacterial and antivirus action of this patent product is carried out in staphylococcus aureus, staphylococcus albus and Escherichia coli three kinds of bacterial strains and influenza A virus FM1 strain.In common pathogenic bacteria and virus, staphylococcus, enteric bacilli and influenza virus are bacterium or the virus with important medical significance; These bacteriums or virus are distributed widely in natural world, as on air, soil, water and article, are also present in humans and animals skin and in the cavity that communicates with the external world; Have the people of 20% ~ 50% to carry pathogenic staphylococcus in crowd, the carrying rate of medical worker can up to 70%; Staphylococcus aureus and Escherichia coli are one of main 6 kinds of bacteriums causing hospital infection, and wherein coli-infection rate is classified as first place.Ministry of Health of China " disinfection technology standard " (version in 2002) i.e. clear stipulaties, in disinfectant germicidal experiment, its microorganism comprises staphylococcus aureus, staphylococcus albus and Escherichia coli etc., and the bacterial classification that air sterilization effect approval test specifies is staphylococcus albus.In addition, in first, second, influenza virus C, influenza A virus is the most popular.Therefore, this patent chooses the three kinds of bacteriums such as staphylococcus aureus, staphylococcus albus and Escherichia coli and influenza A virus FM1 virus that have and represent meaning for evaluation that is antibacterial in following examples and antivirus action, its result can reflect patented product be used for air sterillization and pure and fresh time truth and practical value.
As mentioned above, the present invention is directed to the technical deficiency of existing with fresh air and disinfectant etc., disclosing a kind of take traditional medicine volatile oil as the air disinfectant of principle active component, has antibacterial, antiviral, that fragrance is lasting, economic and practical feature, is of value to the health of human body.
For reaching this object, the present invention proposes the active component of particular combination as air disinfectant of a kind of volatile oil extracted from eucalyptus' leaves or twigs, caryophyllus oil, patchouli oil, Fructus Forsythiae oil and catnip oil.
Above-mentioned composition of the present invention is optionally containing matrix.When containing matrix, the ratio of matrix and volatile oil can change in wider scope, and not impact effect.
And operable matrix is not particularly limited, as long as it can coordinate reaching of the object of the invention.
Above-mentioned air disinfectant can be dispersed in air by modes such as heating, sprayings, or with this air disinfectant filter, rinse air and reach the object of sterilization with fresh air.
Compared with the prior art, tool of the present invention has the following advantages:
1, there is antibacterial and antivirus action, fragrant odour, fresh air, elimination peculiar smell, and fresh and comfortable, all there sensation can be brought to user.The places such as family, office, commercial activity, hotel, compartment can be widely used in, particularly be applied to hospital, not only make people clear-headed, comfortable, and the chance of infection can be greatly reduced.
2, product property is stablized, and durable in use, Costco Wholesale is low, and consumer easily accepts.
3, investment of production is few, workable.This product processes is simple, ripe, and equipment investment is few, easy to operate.
4, prescription is made up of traditional medicine volatile oil, and product safety is reliable.
Specifically, technical scheme provided by the invention is as follows:
1. a preparation, it contains compound volatile oil, and described volatile oil is volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil and catnip oil.
2, the preparation according to technical scheme 1, it is disinfectant preparation with fresh air.
3. the preparation according to technical scheme 1 or 2, is characterized in that described preparation also containing matrix.
4. the preparation according to technical scheme 1 or 2, wherein said preparation is made up of volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil and catnip oil.
5, according to the described preparation of one of technical scheme 1-4, wherein volatile oil extracted from eucalyptus' leaves or twigs is that myrtle blue gum Eucalyptus globulus Labill., canella camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections belong to other plant together through extracting the volatile oil obtained.
6, according to the described preparation of one of technical scheme 1-5, wherein Fructus Forsythiae oil is that the dry fruit of Oleaceae plants capsule of weeping forsythia Forsythia suspensa (Thunb.) Vahl is through extracting the volatile oil obtained.
7, according to the described preparation of one of technical scheme 1-6, wherein caryophyllus oil is that the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb. is through extracting the volatile oil obtained.
8, according to the described preparation of one of technical scheme 1-7, wherein patchouli oil is that the dry stem of labiate Pogostemon cablin Pogostemon cablin (Blanco) Benth. and Ye Jing extract the volatile oil obtained.
9, according to the described preparation of one of technical scheme 1-8, wherein catnip oil is that the herb of labiate schizonepeta Schizonepeta tenuifolia Briq. is through extracting the volatile oil obtained.
10, according to the described preparation of one of technical scheme 1-9, wherein by volume part proportioning is as follows for compound volatile oil each component: volatile oil extracted from eucalyptus' leaves or twigs about 6 ~ 90%, Fructus Forsythiae oil about 3 ~ 70%, caryophyllus oil about 5 ~ 80%, patchouli oil about 1 ~ 70%, catnip oil about 1 ~ 85%.
11, according to the described preparation of one of technical scheme 1-10, wherein compound volatile oil each component is based on the cumulative volume of described compound volatile oil, by volume part proportioning is as follows: volatile oil extracted from eucalyptus' leaves or twigs about 21 ~ 71%, Fructus Forsythiae oil about 8 ~ 48%, caryophyllus oil about 15 ~ 60%, patchouli oil about 3 ~ 47%, catnip oil about 3 ~ 53%.
12, according to the described preparation of one of technical scheme 1-11, wherein compound volatile oil each component is based on the cumulative volume of described compound volatile oil, by volume part proportioning is as follows: volatile oil extracted from eucalyptus' leaves or twigs about 18 ~ 63%, Fructus Forsythiae oil about 5 ~ 19%, caryophyllus oil about 6 ~ 27%, patchouli oil about 6 ~ 16%, catnip oil about 6 ~ 29%.
13, according to the described preparation of one of technical scheme 1-12, wherein by volume part proportioning is as follows for compound volatile oil each component: volatile oil extracted from eucalyptus' leaves or twigs about 43 ~ 52%, Fructus Forsythiae oil about 13 ~ 20%, caryophyllus oil about 21 ~ 30%, patchouli oil about 7 ~ 15%, catnip oil about 7 ~ 15%.
14, according to the described preparation of one of technical scheme 1-13, wherein by volume part proportioning is as follows for compound volatile oil each component: volatile oil extracted from eucalyptus' leaves or twigs about 45%, Fructus Forsythiae oil about 14%, caryophyllus oil about 23%, patchouli oil about 9%, catnip oil about 9%.
15, according to the described preparation of one of technical scheme 1-14, wherein by volume part proportioning is as follows for compound volatile oil each component: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil is about 10: 3: 1: 1: 1.
16, according to the described preparation of one of technical scheme 1-15, wherein by volume part proportioning is as follows for compound volatile oil each component: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil is about 15: 4.5: 7.5: 3: 7.
17, according to the described preparation of one of technical scheme 3-16, its mesostroma is the mixture of kerosene, isopropyl alcohol, absolute ethyl alcohol or its any combination.
18, preparation according to technical scheme 17, its mesostroma each component is based on the cumulative volume of described matrix, and by volume part proportioning is as follows:
Kerosene about 50 ~ 90%,
Isopropyl alcohol or absolute ethyl alcohol about 10 ~ 50%.
19, preparation according to technical scheme 17, by volume part proportioning is as follows for its mesostroma each component:
Kerosene about 71.4%,
Isopropyl alcohol or absolute ethyl alcohol about 28.6%.
20., according to the preparation of one of technical scheme 3-19, is characterized in that described preparation is made up of compound volatile oil and matrix.
21, preparation according to technical scheme 20, wherein, based on described preparation cumulative volume, by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil about 1 ~ 100%, matrix about 0 ~ 99%.
22, preparation according to technical scheme 20, wherein, based on described preparation cumulative volume, by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil about 1 ~ 10%, matrix about 90 ~ 99%.
23, preparation according to technical scheme 22, wherein by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil about 2.5%, matrix about 97.5%.
24, preparation according to technical scheme 22, wherein by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil about 10%, matrix about 90%.
25, in technical scheme 1-24 arbitrary described preparation for making the purposes of disinfecting air with fresh air.
26. according to the purposes of technical scheme 25, and it is dispersed in air, thus filter, rinse air and reach the object of sterilization with fresh air.
27. according to the purposes of technical scheme 25 or 26, and described preparation is dispersed in air by heating and/or spray pattern.
The preparation method of preparation according to any one of 28. technical scheme 1-24:
Described method comprises the step extracting each described component in described compound volatile oil.
29. preparation methods according to technical scheme 28:
Described method comprises the step of each described component in the described compound volatile oil of following extraction:
Adopt steam distillation to extract each component from the raw material of each component, and/or adopt supercritical fluid extraction to extract each component from the raw material of each component.
30. preparation methods according to technical scheme 28 or 29, described method also comprises further:
Measure extracted each component respectively;
Mixed, optionally stirred evenly, to obtain described compound volatile oil.
31. according to the preparation method of technical scheme 30, and described method is further comprising the steps of:
Measure described matrix, the matrix mixing optionally formed by multicomponent, more optionally stir evenly;
And the mixture described matrix being added described each volatile oil is with mixing, is optionally stirred evenly by the mixture of volatile oil and matrix, and described disinfectant preparation with fresh air.
Accompanying drawing explanation
Fig. 1 is the sampled point plane of the embodiment of field experiment.Reference numeral 1 is sampled point.
Embodiment
In this article, the implication of " pure and fresh sterilization " instigates air to present natural aromatic odor and supression and/or the common bacteria killed in air and/or virus.
" volatile oil extracted from eucalyptus' leaves or twigs, caryophyllus oil, patchouli oil, Fructus Forsythiae oil and catnip oil " composition herein, is also referred to as compound volatile oil, herbal mixture volatilization wet goods.
How to prepare in the explanation of each sample following, in compound volatile oil, the raw material of each component is:
Volatile oil extracted from eucalyptus' leaves or twigs: general volatile oil extracted from eucalyptus' leaves or twigs all can use, a concrete example belongs to other plant together through extracting the volatile oil obtained from myrtle blue gum Eucalyptus globulus Labill., canella camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections.(purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, lot number is 20060825 in the commercial goods used in embodiment described herein.)
Fructus Forsythiae oil, general Fructus Forsythiae oil all can use, and concrete example is through extracting the volatile oil obtained from the dry fruit of Oleaceae plants capsule of weeping forsythia Forsythia suspensa (Thunb.) Vahl..(purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, lot number is 20060825 in the commercial goods used in embodiment described herein.)
Caryophyllus oil, general caryophyllus oil all can use, and concrete example is through extracting the volatile oil obtained from the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb..(purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, lot number is 20060825 in the commercial goods used in embodiment described herein.)
Patchouli oil, general patchouli oil all can use, and concrete example extracts from the dry stem of labiate Pogostemon cablin Pogostemon cablin (Blanco) Benth. and Ye Jing the volatile oil obtained.(purchased from Guangzhou pot-pourri Co., Ltd, lot number is 20060901 in the commercial goods used in embodiment described herein.)
Catnip oil, general catnip oil all can use, and concrete example is through extracting the volatile oil obtained from the herb of labiate schizonepeta Schizonepeta tenuifolia Briq..(purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, lot number is 20060825 in the commercial goods used in embodiment described herein.)
Except purchase commercially available prod, steam distillation or supercritical fluid extraction also can be adopted to extract above-mentioned volatile oil according to a conventional method from traditional Chinese medicine, or other existing known extracting method also can be adopted to extract.
Above-mentioned volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil also can extract respectively from the plant of other section, genus.
Wherein volatile oil extracted from eucalyptus' leaves or twigs, patchouli oil carry out quality monitoring by under " Chinese Pharmacopoeia " 2005 editions first the 280th, 272 page of items respectively, caryophyllus oil, by carrying out quality monitoring under " Chinese Pharmacopoeia " 1953 editions first the 7th page of items, is set up Fructus Forsythiae oil voluntarily, the quality standard of catnip oil is monitored.
Be non-limiting example of the present invention below, below fill a prescription (unless specifically stated otherwise) is all parts by volume proportioning.
Embodiment 1
The bacteriostasis (antibacterial around-France) of single and herbal mixture volatile oil
Sample:
Sample 1: volatile oil extracted from eucalyptus' leaves or twigs
Sample 2: Fructus Forsythiae oil
Sample 3: patchouli oil
Sample 4: caryophyllus oil
Sample 5: catnip oil
Sample 6: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 10: 3: 1: 1: 1 ratio.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 625.0ml, Fructus Forsythiae oil 187.5ml, caryophyllus oil 62.5ml, patchouli oil 62.5ml, catnip oil 62.5ml respectively, mixing, stirs evenly, to obtain final product.
Experimental strain:
Staphylococcus aureus (26112), Escherichia coli (44113), staphylococcus albus (26069), by Beijing, Nat'l Pharmaceutical & Biological Products Control Institute provides.
Test method:
By 16 ~ 18 hours liquid cultures of above-mentioned three kinds of bacterium, ordinary flat surface is spread evenly across respectively with aseptic cotton carrier, slightly dry, the drug sensitive test paper having quantitative sample liquid is inhaled with aseptic nipper gripping, be laid in the planar surface of above-mentioned inoculated bacteria, a flat board puts 3 pieces of drug sensitive test papers, puts 37 DEG C of cultivations, next day observed result.Result of the test is in table 1.
Table 1 single medicinal material volatile oil fungistatic effect result of the test (inhibition zone diameter, mm, n=2 ~ 5,
)
Note:
Comprehensive antibacterial index, D=A × 0.3+B × 0.2+C × 0.5, lower same.
N represents experiment number of repetition (lower same).
Result shows, volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, patchouli oil, caryophyllus oil, catnip oil all have inhibitory action in various degree to staphylococcus aureus, staphylococcus albus, colibacillary growth.Wherein caryophyllus oil is the strongest to the effect of staphylococcus aureus, and catnip oil is the strongest to colibacillary effect.
Owing to employing three kinds of bacteriums, and the action intensity of sample to different bacterium is different, be difficult to by simple more different sample to the overall fungistatic effect of the inhibition assess sample of three kinds of bacteriums, therefore need to carry out overall merit, this patent adopts composite index law to evaluate.
Composite index law is a kind of conventional comprehensive analysis method, by giving the fungistatic effect of sample to three kinds of bacteriums different weights, fungistatic effect after considering weight is simply added, namely draws a comprehensive antibacterial index (computing formula is in table 1 footnote).The size of weight is according to the importance of each factor and/or determines, herein because coccus and bacillus represent the different bacterium of two classes respectively, so the weight of coccus and bacillus all gives 0.5 result contribution; Because coccus employs two kinds, wherein staphylococcus aureus pathogenicity is comparatively strong, therefore imparting two kinds of coccus weights are respectively 0.3 and 0.2.Result shows, and the comprehensive antibacterial index of sample 6 (herbal mixture volatile oil) is the highest, shows that the fungistatic effect of herbal mixture volatile oil is better than single volatile oil, and the reasonability of prompting compound and superiority, reflect its novelty and creativeness.
In order to understand further with or without chemiluminescence after single volatile oil composition compound, according to each single medicinal material volatile oil proportion in the respective fungistatic effect of single volatile oil and sample 6, the comprehensive antibacterial index of the theory of computation, result is 10.1.This is starkly lower than the comprehensive antibacterial index 15.0 of sample 6 reality, the synergy created each other after prompting single volatile oil compound composition sample 6, fungistatic effect is strengthened, this has confirmed the conclusion that the preceding paragraph was drawn by compound and the actual measurement of single volatile oil comprehensive antibacterial directly comparing of index, describes novelty and the creativeness of compound.
Embodiment 2
The bacteriostasis (antibacterial around-France) of different ratio herbal mixture volatile oil
Sample:
Sample 7: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 5: 1.5: 2.5: 2: 3 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 357.1ml, Fructus Forsythiae oil 107.1ml, caryophyllus oil 178.6ml, patchouli oil 142.9ml, catnip oil 214.3ml respectively, mixing, stirs evenly, to obtain final product.
Sample 8: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 5: 3: 5: 3: 5 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 238.1ml, Fructus Forsythiae oil 142.9ml, caryophyllus oil 238.1ml, patchouli oil 142.9ml, catnip oil 238.1ml respectively, mixing, stirs evenly, to obtain final product.
Sample 9: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 5: 4.5: 7.5: 4: 7 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 178.6ml, Fructus Forsythiae oil 160.7ml, caryophyllus oil 267.9ml, patchouli oil 142.9ml, catnip oil 250.0ml respectively, mixing, stirs evenly, to obtain final product.
Sample 10: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 10: 1.5: 2.5: 3: 7 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 416.7ml, Fructus Forsythiae oil 62.5ml, caryophyllus oil 104.2ml, patchouli oil 125.0ml, catnip oil 291.7ml respectively, mixing, stirs evenly, to obtain final product.
Sample 11: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 10: 3: 5: 4: 3 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 400.0ml, Fructus Forsythiae oil 120.0ml, caryophyllus oil 200.0ml, patchouli oil 160.0ml, catnip oil 120.0ml respectively, mixing, stirs evenly, to obtain final product.
Sample 12: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 10: 4.5: 7.5: 2: 5 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 344.8ml, Fructus Forsythiae oil 155.2ml, caryophyllus oil 258.6ml, patchouli oil 69.0ml, catnip oil 172.4ml respectively, mixing, stirs evenly, to obtain final product.
Sample 13: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 15: 1.5: 2.5: 4: 5 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 535.7ml, Fructus Forsythiae oil 53.6ml, caryophyllus oil 89.3ml, patchouli oil 142.9ml, catnip oil 178.6ml respectively, mixing, stirs evenly, to obtain final product.
Sample 14: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 15: 3: 5: 2: 7 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 468.8ml, Fructus Forsythiae oil 93.8ml, caryophyllus oil 156.3ml, patchouli oil 62.5ml, catnip oil 218.8ml respectively, mixing, stirs evenly, to obtain final product.
Sample 15: the mixture that volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil form in 15: 4.5: 7.5: 3: 3 ratios.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 454.5ml, Fructus Forsythiae oil 136.4ml, caryophyllus oil 227.3ml, patchouli oil 90.9ml, catnip oil 90.9ml respectively, mixing, stirs evenly, to obtain final product.
Measure the bacteriostasis of above-mentioned sample by the method for embodiment 1, result of the test is in table 2.
Table .2 different ratio Chinese medicine compound prescription volatile oil bacteriostatic test result (inhibition zone diameter, mm, n=3,
)
(ratio that contains in embodiment sample 7 ~ 15 of each volatile oil is, volatile oil extracted from eucalyptus' leaves or twigs 17.9 ~ 53.6%, Fructus Forsythiae oil 5.4 ~ 16.1%, caryophyllus oil 8.9 ~ 26.8%, patchouli oil 6.3 ~ 16.0%, catnip oil 9.1 ~ 29.2%.)
Result shows, the product of above-mentioned 9 formulas all has fungistatic effect.Calculate the antibacterial index of synthesis in theory of each formula, be respectively: 11.4,11.7,11.8,11.5,10.9,11.6,10.9,11.5,11.1, all lower than the comprehensive antibacterial index of actual measurement (in table 2 D row) of each correspondence, illustrate that 9 formulas all create synergy by compatibility in antibacterial, therefore the prescription of these 9 samples has novelty and creativeness too.
In 9 formulas, sample 7,11,12,13,14 has fungistatic effect, and sample 8,9,10 has good fungistatic effect, and sample 15 fungistatic effect is best.In sample 15, the ratio of each volatile oil is: volatile oil extracted from eucalyptus' leaves or twigs 45.5%, Fructus Forsythiae oil 13.6%, caryophyllus oil 22.7%, patchouli oil 9.1%, catnip oil 9.1%.
Above-mentioned test is by volatile oil different proportion orthogonal design L
9(3
4) and the result that obtains, analyze known according to orthogonal design further to its result: the ratio change of volatile oil extracted from eucalyptus' leaves or twigs in formula, little on fungistatic effect impact; The ratio change of Fructus Forsythiae oil, caryophyllus oil and catnip oil has considerable influence to fungistatic effect, fungistatic effect increases along with their ratio increase, ratio the having the greatest impact to fungistatic effect of patchouli oil, in the ratio change of 6.3 ~ 16.0%, fungistatic effect first increases rear reduction.According to the information of above-described embodiment show that the best ratio of theoretic fungistatic effect is 15: 4.5: 7.5: 3: 7, i.e. volatile oil extracted from eucalyptus' leaves or twigs 40.5%, Fructus Forsythiae oil 12.2%, caryophyllus oil 20.3%, patchouli oil 8.1%, catnip oil 18.9%.
Thus optimal proportion and above-mentioned " the ratio change of volatile oil extracted from eucalyptus' leaves or twigs in formula, little on fungistatic effect impact; The ratio change of Fructus Forsythiae oil, caryophyllus oil and catnip oil has considerable influence to fungistatic effect, and fungistatic effect increases and increases along with their ratio " discovery, can reason out, the content range of each volatile oil compositions can at following range changing:
Volatile oil extracted from eucalyptus' leaves or twigs 43 ~ 52%, Fructus Forsythiae oil 13 ~ 20%, caryophyllus oil 21 ~ 30%, patchouli oil 7 ~ 15%, catnip oil 7 ~ 15%; Or
Volatile oil extracted from eucalyptus' leaves or twigs 18 ~ 63%, Fructus Forsythiae oil 5 ~ 19%, caryophyllus oil 6 ~ 27%, patchouli oil 6 ~ 16%, catnip oil 6 ~ 29%, or volatile oil extracted from eucalyptus' leaves or twigs 21 ~ 71%, Fructus Forsythiae oil 8 ~ 48%, caryophyllus oil 15 ~ 60%, patchouli oil 3 ~ 47%, catnip oil 3 ~ 53%;
Or
Volatile oil extracted from eucalyptus' leaves or twigs 6 ~ 90%, Fructus Forsythiae oil 3 ~ 70%, caryophyllus oil 5 ~ 80%, patchouli oil 1 ~ 70%, catnip oil 1 ~ 85%.
Explanation for above-mentioned scope:
Wherein due to the ratio change of volatile oil extracted from eucalyptus' leaves or twigs in formula, little on fungistatic effect impact, the change of the content upper and lower limit of volatile oil extracted from eucalyptus' leaves or twigs can be very large; And the ratio change of Fructus Forsythiae oil, caryophyllus oil and catnip oil, except according to making except legitimate inference near the content of embodiment, consider that its fungistatic effect increases along with their ratio and the fact that increases, therefore its upper limit is actual can larger than in embodiment.
In addition, because in formula, the change of feasible content within the scope of one of each component is successional, therefore any combination between the end points of broad scope recorded of the disclosed point of embodiment and above-mentioned plaintext is also feasible, also namely belongs to the clear and definite scope of disclosure of the present invention.Equally, any coupling between any content range of a component and any content range of other any component is also feasible, belongs to the clear and definite disclosed part of the present invention.
Embodiment 3
The bacteriostasis (antibacterial around-France) of different ratio herbal mixture volatile oil
Sample:
Sample 6: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (10: 3: 1: 1: 1)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 625.0ml, Fructus Forsythiae oil 187.5ml, caryophyllus oil 62.5ml, patchouli oil 62.5ml, catnip oil 62.5ml respectively, mixing, stirs evenly, to obtain final product.
Sample 15: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (15: 4.5: 7.5: 3: 3)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 454.5ml, Fructus Forsythiae oil 136.4ml, caryophyllus oil 227.3ml, patchouli oil 90.9ml, catnip oil 90.9ml respectively, mixing, stirs evenly, to obtain final product.
Sample 16: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (15: 4.5: 7.5: 3: 7)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 405.4ml, Fructus Forsythiae oil 121.6ml, caryophyllus oil 202.7ml, patchouli oil 81.1ml, catnip oil 189.2ml respectively, mixing, stirs evenly, to obtain final product.
Measure the bacteriostasis of above-mentioned sample by the method for embodiment 1, result of the test is in table 3.
Table 3 traditional medicine volatile oil different ratio sample inhibition zone result of the test (inhibition zone diameter, mm, n=3,
)
Result shows, the product of above-mentioned 3 formulas all has fungistatic effect.Sample 16 is the optimization of C/C composites calculated according to the result in example 2, and result shows that its fungistatic effect is better.Calculate the antibacterial index 11.5 of its synthesis in theory, lower than the comprehensive antibacterial index 14.3 of its actual measurement, further demonstrate the synergy that compatibility of drugs produces curative effect enhancing; Sample 16 formula has novelty and creativeness.
Embodiment 4
The bacteriostasis (tube dilution method) of different ratio herbal mixture volatile oil
Sample:
Sample 6: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (10: 3: 1: 1: 1)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 625.0ml, Fructus Forsythiae oil 187.5ml, caryophyllus oil 62.5ml, patchouli oil 62.5ml, catnip oil 62.5ml respectively, mixing, stirs evenly, to obtain final product.
Sample 15: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (15: 4.5: 7.5: 3: 3)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 454.5ml, Fructus Forsythiae oil 136.4ml, caryophyllus oil 227.3ml, patchouli oil 90.9ml, catnip oil 90.9ml respectively, mixing, stirs evenly, to obtain final product.
Sample 16: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (15: 4.5: 7.5: 3: 7)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 405.4ml, Fructus Forsythiae oil 121.6ml, caryophyllus oil 202.7ml, patchouli oil 81.1ml, catnip oil 189.2ml respectively, mixing, stirs evenly, to obtain final product.
Experimental technique:
The each 3ml of sample 6,15,16 is added 1ml Tween 80 respectively makes it dissolve, add nutrient broth to be again mixed with every ml containing crude drug amount and to be respectively 0.30ml, 0.15ml, 0.075ml, 0.0375ml, 0.01875ml, 0.009375ml, 0.004688ml, 0.002344ml, 0.001172ml, the dilute sample of totally 9 drug concentrations, often pipe total amount 1ml, heat sterilization.
Control tube: bacterial classification contrasts is the medium Accelerated Life bacterium of not drug containing; Drug control is the liquid of not Accelerated Life bacterium.
It is 10 that each concentration pipe of every drug row liquid and bacterial classification control tube add concentration respectively
6cfuml
-1test organisms liquid (8 hours cultures) 0.1ml, cultivate 24 hours, then each concentration oese got a ring streak inoculation on Solid nutritional agar plate for 37 DEG C, cultivate 24 hours observed results for 37 DEG C.Be that each pipe of index naked-eye observation has asepsis growth with turbidity; With the naked eye observe each flat board with or without colony growth.
Judge minimal inhibitory concentration (MIC), refer to the minimum drug concentration contained by complete inhibition test growth.
Judge minimal bactericidal concentration (MBC), refer to kill the minimum drug concentration contained by test strain growth completely.
Result of the test is in table 4.
Table 4 different ratio herbal mixture volatile oil is to MIC and MBC measurement result (the μ lml of 3 kinds of bacteriums
-1)
MIC and MBC is two important indicators of the antibacterial and sterilization of medicine, and the antibacterial effect of the less explanation medicine of its value is better, utilizes this two parameters, evaluates the concentration of sample 6,15,16 and its fungistatic effect.Result shows, above-mentioned 3 samples all have Antibacteria effect in various degree to test strain, and the MIC of each bacterial classification is between 2.34 μ lml
-1~ 75.0 μ lml
- 1between, the MBC of each bacterial classification is between 4.69 μ lml
-1~ 75.0 μ lml
-1between, wherein the comprehensive bactericidal index of the MBC of sample 16 is minimum, and its MIC and sample 15 is close, and illustrate that its comprehensive bactericidal effect is best, comprehensive fungistatic effect is also better.
Embodiment 5
The antivirus action of different ratio herbal mixture volatile oil
Sample:
Sample 6: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (10: 3: 1: 1: 1)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 625.0ml, Fructus Forsythiae oil 187.5ml, caryophyllus oil 62.5ml, patchouli oil 62.5ml, catnip oil 62.5ml respectively, mixing, stirs evenly, to obtain final product.
Sample 15: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (15: 4.5: 7.5: 3: 3)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 454.5ml, Fructus Forsythiae oil 136.4ml, caryophyllus oil 227.3ml, patchouli oil 90.9ml, catnip oil 90.9ml respectively, mixing, stirs evenly, to obtain final product.
Sample 16: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil (15: 4.5: 7.5: 3: 7)
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 405.4ml, Fructus Forsythiae oil 121.6ml, caryophyllus oil 202.7ml, patchouli oil 81.1ml, catnip oil 189.2ml respectively, mixing, stirs evenly, to obtain final product.
Positive control drug virazole, lot number 0301006, by Hubei, medical professionals provided.
Seed culture of viruses:
Influenza A virus FM1 strain is provided by Inst. of Medicinal Biological Technology, Chinese Academy of Medical Sciences, in MDCK MDCK (Madin-Darby Canine Kidney cell, Madin-Darby canine kidney cell) go down to posterity, with pathology and and chicken erythrocyte Hemagglutination Method judge its titre, at 37 DEG C, 5%CO
2cultivate in incubator.
Experimental technique:
Require to carry out by " study of tcm new drug guide " (bureau of drug policy & administration of Ministry of Health of the People's Republic of China, version in 1994), above Viral Assay carries out on 96 hole micro plates, every hole 0.1ml.Cell number is 1 × 10
5after individual/ml grows up to individual layer, by medicinal maintenance medium be diluted to 4 variable concentrations, add in cell hole by variable concentrations traditional Chinese medicine sample after jettisoning original fluid, every hole 0.1ml, the minimum dilution factor not occurring pathology with cell is for this liquid is to cytotoxic boundary.On mdck cell, measure the TCID of challenge virus simultaneously
50(50%tissue culture infective dose, tissue culture infective dose).Virus control, cell controls, drug toxicity control and positive drug is established to contrast (virazole) during test.Put 37 DEG C, 5%CO
2cultivate in incubator.Every day observes pathology and record under inverted microscope.When virus control porocyte occurs +++ during pathology, continue observation and then stop for 7 days.Cell occurs that the degree of pathology is by 6 grade standard records :-: Growth of Cells is normal, occurs without pathology; " ± ": cytopathy is less than 10% of whole cell monolayer; "+": cytopathy is less than 25% of whole cell monolayer; " ++ ": cytopathy is less than 50% of whole cell monolayer; " +++ ": cytopathy is less than 75% of whole cell monolayer; " ++++": cytopathy accounts for more than 75% of whole cell monolayer.Result of the test is in table 5.
Table 5 traditional medicine volatile oil different ratio sample is to influenza A virus FM1 inhibitory action
Note: "+" suppresses "-" to viral unrestraint effect to virus completely
Result shows, at the viral TCID of infection
50under 100 dosage, virazole 100 μ gml
-1have obvious inhibitory action to FM1, under sub-toxic concentration, No. 15, sample is at 160 μ lml
-1certain inhibitory action is had to FM1.Interpret sample 15 also has the effect of the common virus suppressed in air, has the effect of air sterillization.(in embodiment subsequently, selecting the formula of sample 15 to carry out the object of one of them continuation research)
Embodiment 6
Simulation field test
Sample:
Chinese medicine air disinfectant 1: formulated by 1: 9 by herbal mixture volatile oil and matrix, wherein herbal mixture volatile oil be volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil by 15: 4.5: 7.5: 3: 3 composition, matrix is that kerosene and absolute ethyl alcohol form by 2.5: 1.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 45.5ml, Fructus Forsythiae oil 13.6ml, caryophyllus oil 22.7ml, patchouli oil 9.1ml, catnip oil 9.1ml respectively, mixing, stirs evenly, obtains herbal mixture volatile oil, for subsequent use; Measure kerosene 642.9ml and absolute ethyl alcohol 257.1ml respectively, mixing, stirs evenly, adds above-mentioned mixture of volatile oil, and mixing, stirs evenly, to obtain final product.
Equipment:
Test strain: staphylococcus albus 8032, is provided by Military Medical Science Institute.Bacterial classification algebraically was 6th ~ 8 generations, prepared bacteria suspension with ordinary broth.
Aerosol generator, more than 90% particle Φ≤5 μm.
Aerosol chamber's volume is 10m
3.
Sampler: FA-1 type impacting type air microorganism sampler.
Method:
Test basis: Ministry of Public Health's " disinfection technology standard " (version in 2002) 2.1.3.
Test temperature 23 ~ 24 DEG C, relative moisture 50 ~ 58%.
Microbiological contamination sprayer flow is 21.7L/min, spray time 5min, stirs 5min, leaves standstill 5min.
Sterilization method: with 2ml/m during test
3amount, with QPQ-2300 type electric motion aerosol atomizer, sprayed in laboratory, disinfective action 60min post-sampling.Control group deionized water replaces disinfectant, and other operate same experimental group.Test repetition 3 times.
Adopt FA-1 type impacting type air microorganism sampler, sampling flow is 28.3L/min, and in room central authorities, distance ground 1.0m establishes a point sampling.Sample when disinfection time is 0min, 60min, the control group sampler samples time is respectively 5s, 5s, and the experimental group sampler samples time is respectively 5s, 1min, measures different time and deposits viable count, be calculated as follows killing rate after sampling:
N
t: the natural decay rate of bacterium in air;
V
0with V
t: be respectively before control group on-test and the microbe contents in air of different time in process of the test;
K
t: disinfect the killing rate to bacterium in air;
V
0' with V
t': be respectively experimental group disinfect before and the microbe contents in air of different time in disinfecting process.
The results are shown in Table 6.
Table 6 Chinese medicine air disinfectant 1 is to the staphylococcic killing effect of white
Negative control: the equal asepsis growth of dilution, medium.
Result shows, Chinese medicine air disinfectant 1 disinfective action 60min, and to the staphylococcic killing rate of white, 3 test killing rates are all more than 96.30%.Illustrate that Chinese medicine air disinfectant 1 can kill the common bacteria in air, there is the effect of air disinfection.
Embodiment 7
Field trial
Sample:
Chinese medicine air disinfectant 2: formulated by 1: 39 by herbal mixture volatile oil and matrix, wherein herbal mixture volatile oil be volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil by 15: 4.5: 7.5: 3: 3 composition, matrix is that kerosene and isopropyl alcohol form by 2.5: 1.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 11.4ml, Fructus Forsythiae oil 3.4ml, caryophyllus oil 5.7ml, patchouli oil 2.3ml, catnip oil 2.3ml respectively, mixing, stirs evenly, obtains herbal mixture volatile oil, for subsequent use; Measure kerosene 696.4ml and isopropyl alcohol 278.6ml respectively, mixing, stirs evenly, adds above-mentioned mixture of volatile oil, and mixing, stirs evenly, to obtain final product.
Chinese medicine air disinfectant 3: formulated by 1: 9 by herbal mixture volatile oil and matrix, wherein herbal mixture volatile oil be volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil by 15: 4.5: 7.5: 3: 3 composition, matrix is that kerosene and isopropyl alcohol form by 2.5: 1.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 45.5ml, Fructus Forsythiae oil 13.6ml, caryophyllus oil 22.7ml, patchouli oil 9.1ml, catnip oil 9.1ml respectively, mixing, stirs evenly, obtains herbal mixture volatile oil, for subsequent use; Measure kerosene 642.9ml and isopropyl alcohol 257.1ml respectively, mixing, stirs evenly, adds above-mentioned mixture of volatile oil, and mixing, stirs evenly, to obtain final product.
Experimental enviroment and instrument:
Room volume: 70m
3
Temperature and humidity: room temperature: 31 DEG C, room relative moisture: 85%
Spraying instrument: electric motion aerosol atomizer, model: DQP-600, producer: Beijing development in science and technology Co., Ltd of Song Yuan Huaxing
Sterilization and sampling:
Sampled point is as shown below, carries out 2 samplings altogether, when the sampling time, point was respectively 0h, during 2h.
The method of sampling: on the sampled point of 5 as shown in Figure 1, it is dull and stereotyped that each point puts two diameter 9cm, and height of sampling is 1.0m, opens culture dish, exposes after 10 minutes, is built by lid, and sample is cultivated 24 hours in 37 DEG C, calculates clump count and be calculated as follows extinction rate.
Sterilizing operation: undertaken by the dosage of every cubic metre of 1ml, be evenly sprayed on room with electric motion aerosol atomizer, sprayed, sterilization 2h.
Statistical Identifying Method:
The results are shown in Table 7,8.
Disinfection Effect under the on-the-spot air-tight state of table 7 Chinese medicine air disinfectant 2
Disinfection Effect under the on-the-spot air-tight state of table 8 Chinese medicine air disinfectant 3
Result shows, and at the scene under air-tight state, carries out pure and fresh sterilization with air in Chinese medicine air disinfectant 2,3 pairs of spaces, bacterial number in air can be made to reduce about 71% and 76%, reduce and have significant after sterilization.Illustrate that Chinese medicine air disinfectant 2,3 can kill the bacterium in air, there is air and to disappear the effect of bacterium.
According to the data 1: 9 and 1: 39 of above-described embodiment, and effect is visible, and the ratio of matrix and volatile oil can change in wider scope, and what does not have affect on effect.All can the present invention be implemented in following scope:
Compound volatile oil > 0 ~≤100%, matrix 0 ~ 99%; Wherein " > 0 " expression is greater than 0.
Compound volatile oil 1 ~ 100%, matrix 0 ~ 99%;
Or compound volatile oil 1 ~ 10%, matrix 90 ~ 99%.
And for the selection of operable matrix, be not particularly limited, as long as it can coordinate reaching of the object of the invention, this is that those skilled in the art simply can measure selection.The example of matrix includes but not limited to as such as kerosene, isopropyl alcohol, absolute ethyl alcohol etc., or the mixture of its any combination.
Embodiment 8
Acute oral toxicity test
Test objective:
Observe the toxic reaction produced after Chinese medicine air disinfectant once gives animal, evaluate this pure and fresh disinfectant when practical application to the safety of human body.
Sample:
Chinese medicine air disinfectant 2: formulated by 1: 39 by herbal mixture volatile oil and matrix, wherein herbal mixture volatile oil be volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil, catnip oil by 15: 4.5: 7.5: 3: 3 composition, matrix is that kerosene and isopropyl alcohol form by 2.5: 1.
Preparation method: measure volatile oil extracted from eucalyptus' leaves or twigs 11.4ml, Fructus Forsythiae oil 3.4ml, caryophyllus oil 5.7ml, patchouli oil 2.3ml, catnip oil 2.3ml respectively, mixing, stirs evenly, obtains herbal mixture volatile oil, for subsequent use; Measure kerosene 696.4ml and isopropyl alcohol 278.6ml respectively, mixing, stirs evenly, adds above-mentioned mixture of volatile oil, and mixing, stirs evenly, to obtain final product.
During test is Jin Longyu
(date of manufacture: 2007-01-30) (certainly also can using of other) dilution.
Experimental animal:
NIH (National Institute of Health, NIH) is that (body weight 18 ~ 22g, is provided by Guangdong Province's Experimental Animal Center small white mouse for the quality certification number: 0027327), male and female half and half.
Test method:
Get NIH system mouse 20, male and female half and half, body weight 18 ~ 22g.Animal adaptability raises 2d.Animal overnight fasting before administration, freely to drink water.Appropriate peanut oil dilution 5.0287g Chinese medicine air disinfectant 2 to 20ml is got during test, by 20ml/kg body weight and dosage 5000mg/kg body weight gavage, at once reaction of animals is observed, Continuous Observation two weeks, record animal toxicity response situation and dead distribution, perform an autopsy on sb. to dead animal, record pathology situation, judges LD simultaneously
50whether be greater than 5000mg/kg body weight.
Result and conclusion:
Result of the test shows: Chinese medicine air disinfectant 2 is the disposable gavage of 5000mg/kg body weight according to dosage, and animal does not occur that overt toxicity reacts.After administration, the reaction main manifestations of animal is movable minimizing, and minority occurs excitement, beats; After administration, 2 hours animal activities recover normal substantially; Occur dead without animal.Therefore judge its LD
50be greater than 5000mg/kg body weight.
Toxicity assessment standard according in Ministry of Health of the People's Republic of China's " disinfection technology standard " (version in 2002):
LD
50be greater than the true border of 5000mg/kg body severe one nontoxic;
LD
50for 501mg/kg ~ 5000mg/kg body severe one belongs to low toxicity;
LD
50for 51mg/kg ~ 500mg/kg body severe one belongs to medium poison;
LD
50for 1mg/kg ~ 50mg/kg body severe one belongs to high poison;
LD
50be less than 1mg/kg body severe one and belong to severe toxicity.
Due to the LD of this Chinese medicine air disinfectant 2
50be greater than 5000mg/kg body weight, can think and belong to non-toxic product.
Conclusion
Chinese medicine air disinfectant has antibacterial, sterilization significantly and even antiviral activity, its prescription is novel, obviously can suppress the growth of air middle school common pathogen, there is not animal dead and obvious toxic reaction in the oral 5000mg/kg of animal, point out it safe and effective, its smell is pure and fresh, can be used for air sterillization and pure and fresh.
In addition, embodiment is herein all non-limiting example, involved numerical value, unless specifically stated otherwise, also can be about value.Any combination between the end points of the scope of the disclosed point of embodiment and summary is also scope disclosed by the invention; Any coupling between any content range of a component and any content range of other component is also a part disclosed by the invention.Also a part of the present invention is belonged to according to change of the present invention.
Claims (1)
1. a disinfectant preparation with fresh air, it contains compound volatile oil, and described volatile oil is volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil and catnip oil;
Wherein, each component of described compound volatile oil, based on the cumulative volume of described compound volatile oil, by volume the proportioning of part is as follows:
Volatile oil extracted from eucalyptus' leaves or twigs 17.9 ~ 53.6%,
Fructus Forsythiae oil 5.4 ~ 16.1%,
Caryophyllus oil 8.9 ~ 26.8%,
Patchouli oil 6.3 ~ 16.0%,
Catnip oil 9.1 ~ 29.2%.
2. preparation according to claim 1, is characterized in that described preparation also containing matrix.
3. preparation according to claim 1, wherein said preparation is made up of volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil and catnip oil.
4, preparation according to claim 1, wherein volatile oil extracted from eucalyptus' leaves or twigs is myrtle blue gum
eucalyptus globuluslabill., canella camphor tree
cinnamomum camphora(L.) volatile oil that the other plant that Presl or above-mentioned two sections belong to together obtains through extraction.
5, preparation according to claim 1, wherein Fructus Forsythiae oil is the Oleaceae plants capsule of weeping forsythia
forsythia suspensa(Thunb.) dry fruit of Vahl is through extracting the volatile oil obtained.
6, preparation according to claim 1, wherein caryophyllus oil is plant clove of myrtaceae
eugenia caryophyllatathunb. dry flower is through extracting the volatile oil obtained.
7, preparation according to claim 1, wherein patchouli oil is labiate Pogostemon cablin
pogostemon cablin(Blanco) the dry stem of Benth. and Ye Jing extract the volatile oil obtained.
8, preparation according to claim 1, wherein catnip oil is labiate schizonepeta
schizonepeta tenuifoliabriq. herb is through extracting the volatile oil obtained.
9. preparation according to any one of claim 1-3, wherein
Volatile oil extracted from eucalyptus' leaves or twigs is myrtle blue gum
eucalyptus globuluslabill., canella camphor tree
cinnamomum camphora(L.) volatile oil that the other plant that Presl or above-mentioned two sections belong to together obtains through extraction;
Fructus Forsythiae oil is the Oleaceae plants capsule of weeping forsythia
forsythia suspensa(Thunb.) dry fruit of Vahl is through extracting the volatile oil obtained;
Caryophyllus oil is plant clove of myrtaceae
eugenia caryophyllatathunb. dry flower is through extracting the volatile oil obtained;
Patchouli oil is labiate Pogostemon cablin
pogostemon cablin(Blanco) the dry stem of Benth. and Ye Jing extract the volatile oil obtained; And
Catnip oil is labiate schizonepeta
schizonepeta tenuifoliabriq. herb is through extracting the volatile oil obtained.
10, a disinfectant preparation with fresh air, it contains compound volatile oil, and described volatile oil is volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil and catnip oil;
Wherein, each component of described compound volatile oil, based on the cumulative volume of described compound volatile oil, by volume the proportioning of part is as follows:
Volatile oil extracted from eucalyptus' leaves or twigs 45%, Fructus Forsythiae oil 14%, caryophyllus oil 23%, patchouli oil 9%, catnip oil 9%;
Wherein
Volatile oil extracted from eucalyptus' leaves or twigs is myrtle blue gum
eucalyptus globuluslabill., canella camphor tree
cinnamomum camphora(L.) volatile oil that the other plant that Presl or above-mentioned two sections belong to together obtains through extraction;
Fructus Forsythiae oil is the Oleaceae plants capsule of weeping forsythia
forsythia suspensa(Thunb.) dry fruit of Vahl is through extracting the volatile oil obtained;
Caryophyllus oil is plant clove of myrtaceae
eugenia caryophyllatathunb. dry flower is through extracting the volatile oil obtained;
Patchouli oil is labiate Pogostemon cablin
pogostemon cablin(Blanco) the dry stem of Benth. and Ye Jing extract the volatile oil obtained; And/or
Catnip oil is labiate schizonepeta
schizonepeta tenuifoliabriq. herb is through extracting the volatile oil obtained.
11, a disinfectant preparation with fresh air, it contains compound volatile oil, and described volatile oil is volatile oil extracted from eucalyptus' leaves or twigs, Fructus Forsythiae oil, caryophyllus oil, patchouli oil and catnip oil;
Wherein, each component of described compound volatile oil, based on the cumulative volume of described compound volatile oil, by volume the proportioning of part is as follows:
Volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil is 10:3:1:1:1;
Wherein
Volatile oil extracted from eucalyptus' leaves or twigs is myrtle blue gum
eucalyptus globuluslabill., canella camphor tree
cinnamomum camphora(L.) volatile oil that the other plant that Presl or above-mentioned two sections belong to together obtains through extraction;
Fructus Forsythiae oil is the Oleaceae plants capsule of weeping forsythia
forsythia suspensa(Thunb.) dry fruit of Vahl is through extracting the volatile oil obtained;
Caryophyllus oil is plant clove of myrtaceae
eugenia caryophyllatathunb. dry flower is through extracting the volatile oil obtained;
Patchouli oil is labiate Pogostemon cablin
pogostemon cablin(Blanco) the dry stem of Benth. and Ye Jing extract the volatile oil obtained; And/or
Catnip oil is labiate schizonepeta
schizonepeta tenuifoliabriq. herb is through extracting the volatile oil obtained.
12, according to the described preparation of one of claim 4-8, wherein by volume part proportioning is as follows for compound volatile oil each component: volatile oil extracted from eucalyptus' leaves or twigs: Fructus Forsythiae oil: caryophyllus oil: patchouli oil: catnip oil is 15:4.5:7.5:3:7.
13, preparation according to claim 2, its mesostroma is the mixture of kerosene, isopropyl alcohol, absolute ethyl alcohol or its any combination.
14, according to preparation described in claim 13, its mesostroma each component is based on the cumulative volume of described matrix, and by volume part proportioning is as follows:
Kerosene 50 ~ 90%,
Isopropyl alcohol or absolute ethyl alcohol 10 ~ 50%.
15, according to preparation described in claim 13, by volume part proportioning is as follows for its mesostroma each component:
Kerosene 71.4%,
Isopropyl alcohol or absolute ethyl alcohol 28.6%.
16. preparations according to claim 2, is characterized in that described preparation is made up of compound volatile oil and matrix.
17, according to preparation described in claim 16, wherein, based on described preparation cumulative volume, by volume part proportioning is as follows: compound volatile oil 1 ~ 100% for compound volatile oil and the matrix that can contain, matrix 0 ~ 99%.
18, according to preparation described in claim 16, wherein, based on described preparation cumulative volume, by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil 1 ~ 10%, matrix 90 ~ 99%.
19, according to preparation described in claim 18, wherein by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil 2.5%, matrix 97.5%.
20, according to preparation described in claim 18, wherein by volume part proportioning is as follows for compound volatile oil and matrix: compound volatile oil 10%, matrix 90%.
21, in claim 1-20 arbitrary described preparation for making the purposes of sterilization with fresh air.
22. purposes according to claim 21, it is dispersed in air, thus filter, rinse air and reach the object making sterilization with fresh air.
23. want the purposes of 21 or 22 according to right, and described preparation is dispersed in air by heating and/or spray pattern.
The preparation method of preparation according to any one of 24. claim 1-20:
Described method comprises the step extracting each described component in described compound volatile oil.
25. preparation methods according to claim 24:
Described method comprises the step of each described component in the described compound volatile oil of following extraction:
Adopt steam distillation to extract each component from the raw material of each component, and/or adopt supercritical fluid extraction to extract each component from the raw material of each component.
26. preparation methods according to claim 24 or 25, described method also comprises further:
Measure extracted each component respectively;
Mixed, optionally stirred evenly, to obtain described compound volatile oil.
27. preparation methods according to claim 26, described method is further comprising the steps of:
Measure described matrix, the matrix mixing optionally formed by multicomponent, more optionally stir evenly;
And the mixture described matrix being added described each volatile oil is with mixing, is optionally stirred evenly by the mixture of volatile oil and matrix, and described disinfectant preparation with fresh air.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710162111.2A CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710162111.2A Division CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
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| CN103461394B true CN103461394B (en) | 2015-07-22 |
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|---|---|---|---|
| CN200710162111.2A Active CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
| CN201310283575.4A Active CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710162111.2A Active CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
Country Status (2)
| Country | Link |
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| CN (2) | CN101455850B (en) |
| HK (1) | HK1192416A1 (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455850B (en) * | 2007-12-14 | 2014-02-12 | 永正顾问有限公司 | Air disinfectant |
| WO2010094151A1 (en) * | 2009-02-20 | 2010-08-26 | 永正顾问有限公司 | Disinfectant making air fresh and preparation method and use thereof |
| CN103191183A (en) * | 2012-07-30 | 2013-07-10 | 成都中医药大学 | Use of palchouli oil in preparation of antiviral drug |
| CN103891738A (en) * | 2012-12-31 | 2014-07-02 | 青岛锦涟鑫商贸有限公司 | Indoor air freshening disinfectant |
| CN104996503B (en) * | 2015-07-22 | 2017-09-29 | 四川农业大学 | It is a kind of for Chinese medicine compound prescription air sterillization spray of plant and preparation method thereof |
| CN105412965A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Sandalwood air freshener and preparation method thereof |
| CN105412966A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Chinese herb air freshener and preparation method thereof |
| CN105477662A (en) * | 2015-11-30 | 2016-04-13 | 陶超杰 | Jojoba oil air freshener and preparation method thereof |
| CN105561363A (en) * | 2016-01-21 | 2016-05-11 | 广西大学 | Natural antibacterial air freshener and preparation method thereof |
| CN106943821A (en) * | 2016-10-12 | 2017-07-14 | 贾明忠 | A kind of residential block nebulization dust system and its sprinkling solution additive |
| CN107343865B (en) * | 2017-08-03 | 2020-09-01 | 成都煜泉绿健科技有限公司 | Compound volatile oil and preparation method and application thereof |
| CN108721671A (en) * | 2018-06-13 | 2018-11-02 | 李帛昀 | Student's air freshener with functions of refreshing brain and restoring consciousness and preparation method thereof |
| CN108605999A (en) * | 2018-06-25 | 2018-10-02 | 杭州光启医疗科技发展有限公司 | A kind of medical bactericidal liquid and preparation method thereof |
| CN111109302B (en) * | 2020-02-22 | 2021-04-06 | 深圳超微颗粒产业有限责任公司 | Antimicrobial aerosol and toxicant exposure spray suspended particle spray and preparation method thereof |
| CN111493103A (en) * | 2020-05-21 | 2020-08-07 | 泓麟生物科技(广州)有限公司 | Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application |
| CN112205430B (en) * | 2020-09-07 | 2022-01-18 | 恩民医药科技有限公司 | Traditional Chinese medicine extract composition and preparation method and application thereof |
| CN112021695A (en) * | 2020-09-08 | 2020-12-04 | 东莞市华佑医疗科技有限公司 | Medical mask of compound Chinese medicinal aromatherapy essential oil for resisting virus and influenza |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631447A (en) * | 2004-12-30 | 2005-06-29 | 浙江理工大学 | Air disinfecting fumigant and its using method |
| CN1742752A (en) * | 2005-10-08 | 2006-03-08 | 晏四平 | Atomizing agent containing medicinal plant oil |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1128672A (en) * | 1995-02-11 | 1996-08-14 | 卞伟 | Agent for purifying and refreshing air |
| CN1156540A (en) * | 1996-09-24 | 1997-08-13 | 南召县供销合作社联合社 | Air pure and fresh disinfectant using folium artemisiae argyi oil as main and preparing process thereof |
| CN1179649C (en) * | 2003-03-27 | 2004-12-15 | 李汉文 | Mouldproof and bacteriostatic aromatic oil product series and its production process |
| CN101455850B (en) * | 2007-12-14 | 2014-02-12 | 永正顾问有限公司 | Air disinfectant |
-
2007
- 2007-12-14 CN CN200710162111.2A patent/CN101455850B/en active Active
- 2007-12-14 CN CN201310283575.4A patent/CN103461394B/en active Active
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2014
- 2014-06-20 HK HK14105895.9A patent/HK1192416A1/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631447A (en) * | 2004-12-30 | 2005-06-29 | 浙江理工大学 | Air disinfecting fumigant and its using method |
| CN1742752A (en) * | 2005-10-08 | 2006-03-08 | 晏四平 | Atomizing agent containing medicinal plant oil |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1192416A1 (en) | 2014-08-22 |
| CN101455850A (en) | 2009-06-17 |
| CN103461394A (en) | 2013-12-25 |
| CN101455850B (en) | 2014-02-12 |
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