CN111493103A - Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application - Google Patents
Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application Download PDFInfo
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- CN111493103A CN111493103A CN202010434640.9A CN202010434640A CN111493103A CN 111493103 A CN111493103 A CN 111493103A CN 202010434640 A CN202010434640 A CN 202010434640A CN 111493103 A CN111493103 A CN 111493103A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
Abstract
The invention relates to the technical field of air disinfection, in particular to a traditional Chinese medicine composition, a traditional Chinese medicine air freshening disinfectant and application. The traditional Chinese medicine composition disclosed by the invention is a pure traditional Chinese medicine formula, comprises patchouli oil, schizonepeta oil, clove oil, blumea oil and zedoary turmeric oil, and has the characteristics of bacteriostasis, antivirus, lasting fragrance and economy and applicability. The Chinese medicinal composition can be mixed with matrix to prepare air refreshing disinfectant, and can be diffused into air by heating, spraying, natural volatilization and other modes, or the air refreshing disinfectant can be used for filtering and washing air to achieve the purpose of air refreshing and disinfecting.
Description
Technical Field
The invention belongs to the technical field of air disinfection, and particularly relates to a traditional Chinese medicine composition, a traditional Chinese medicine air refreshing disinfectant and application.
Background
The environment is increasingly worsened, harmful substances in the air are increased, and the emotion, health and working efficiency of people are affected by peculiar smell and air pollution. In recent years, influenza is frequently encountered, the influenza is mainly spread by cross infection of droplets and air, and the cross infection can be effectively prevented and controlled by disinfecting the air in public places, so that the large-scale spread of viruses is inhibited. At present, most of air-refreshing disinfectants sold in the market comprise artificially synthesized chemical sterilizing agents, aromatic agents and other components. Frequent exposure to air containing chemical sterilants can be harmful to human health. In addition, the fragrance contained in the air freshener functions to cover the odor by emitting fragrance, rather than reacting with the odor-causing gas in the air. In addition, the aromatic can also cause harm to the nervous system of people and stimulate respiratory mucosa of children. Therefore, it is important to develop safe and effective natural air-freshening and sterilizing products for purifying indoor air.
Disclosure of Invention
In view of the above, the invention aims to provide a traditional Chinese medicine composition, a traditional Chinese medicine air freshening disinfectant and an application thereof. The traditional Chinese medicine composition can be mixed with a substrate to prepare an air freshening disinfectant, and the air freshening disinfectant is diffused into air through heating, spraying, natural volatilization and other modes, or the air freshening disinfectant is used for filtering and washing air so as to achieve the purpose of air freshening disinfection.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a traditional Chinese medicine composition which comprises patchouli oil, catnip oil, clove oil, blumea oil and zedoary turmeric oil.
Preferably, the mass ratio of the patchouli oil, the catmint oil, the clove oil, the blumea oil and the zedoary turmeric oil in the traditional Chinese medicine composition is 1-4:1-4:1-4:5-10: 5-10.
Preferably, the weight ratio of the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the schizonepeta oil in the traditional Chinese medicine composition is 10:10:2:1: 4.
The invention also provides a traditional Chinese medicine air freshening disinfectant, which comprises the traditional Chinese medicine composition and a substrate; the mass ratio of the traditional Chinese medicine composition to the matrix is 1-100: 0 to 99.
Preferably, in the traditional Chinese medicine air freshening disinfectant, the mass ratio of the traditional Chinese medicine composition to the matrix is 1: 9.
Preferably, in the traditional Chinese medicine air freshening disinfectant, the mass ratio of the traditional Chinese medicine composition to the matrix is 1: 49.
In the present invention, preferably, in the traditional Chinese medicine air freshening disinfectant, the matrix comprises absolute ethyl alcohol.
The invention also provides application of the traditional Chinese medicine air freshening disinfectant in killing and/or inhibiting microorganisms.
Preferably, the microorganisms include staphylococcus aureus, escherichia coli, staphylococcus albus, and influenza a virus.
The invention provides a traditional Chinese medicine composition which is a pure traditional Chinese medicine formula and has the characteristics of bacteriostasis, antivirus, lasting fragrance, economy and applicability. Compared with the prior art, the traditional Chinese medicine air freshening disinfectant has the effect of killing or inhibiting various microorganisms, especially pathogenic bacteria or virus medicines commonly seen in the air. The traditional Chinese medicine air freshening disinfectant can be diffused into the air in the modes of heating, spraying, natural volatilization and the like, or the traditional Chinese medicine air freshening disinfectant is used for filtering and washing the air so as to achieve the aim of air freshening disinfection.
Detailed Description
The invention provides a traditional Chinese medicine composition which comprises patchouli oil, catnip oil, clove oil, blumea oil and zedoary turmeric oil.
Wherein the oleum herba Pogostemonis is volatile oil extracted from dry aerial parts of herba Pogostemon cablin (Blanco) Benth of Labiatae by steam distillation. Has effects of eliminating phlegm, relieving cough and asthma, etc., and contains patchoulenone with antibacterial activity. The source of patchouli oil is not particularly limited in the present invention, and any patchouli oil obtained by extraction methods known in the art or commercially available can be used in the present invention. Preferably, in the present invention, the patchouli oil complies with the standard of the chinese pharmacopoeia 2015 year edition.
In the invention, the catmint oil is light yellow volatile oil extracted from the whole plant of dried catmint [ schizonepeta tenuifolia (Benth.) Briq. ] by steam distillation, mainly contains monoterpene and sesquiterpene components, and has various effects of anti-inflammation, anti-virus, anti-allergy and anti-tumor. The source of catmint oil is not particularly limited in the present invention, and any catmint oil obtained by extraction known in the art or commercially available can be used in the present invention. Preferably, in the present invention, the catmint oil contains more than 70% of the total amount of the volatile oil of pulegone and menthone.
The clove oil is volatile oil extracted from dry buds of myrtaceae plant clove (Eugenia caryophyllata Thunb) by steam distillation, and has the effects of antisepsis, antibiosis, pain relief and the like.
In the invention, the blumea oil is volatile oil contained in dry leaves or fresh leaves of blumea balsamifera (Artemisia argyi) of Compositae, the main active ingredients of the blumea oil are typhinol-4 (Terphinenol-4) and α -Terpinenol (α -Terphinenol). the blumea oil is a natural antibacterial agent, has wide antibacterial spectrum and antibacterial activity on bacteria, fungi and viruses, the source of the blumea oil is not particularly limited, and the blumea oil obtained by any extraction method known in the prior art or commercially available blumea oil can be used in the invention.
In the invention, the zedoary turmeric oil is volatile oil extracted from zedoary turmeric (zedoary turmeric) through steam distillation. The external application has the efficacy of dispelling wind and fire and has obvious effect on fungal infection. The source of zedoary turmeric oil in the present invention is not particularly limited, and any commercially available zedoary turmeric oil or zedoary turmeric oil obtained by extraction methods known in the art can be used in the present invention. Preferably, in the invention, the zedoary turmeric oil meets the 2015 standard of Chinese pharmacopoeia.
Patchouli oil is a natural spice and also an antibacterial agent, has the functions of relieving exterior syndrome and eliminating dampness, regulating qi and regulating the middle warmer, diminishing inflammation and sterilizing, can protect wounds or ulcers of skin from infection, can kill fungi, and has a certain treatment effect on beriberi infection. The schizonepeta oil has antipyretic, antibacterial and anti-inflammatory effects. The blumea oil has the effects of clearing away heat and toxic materials, resisting allergy, eliminating dampness, treating eczema and the like. Zedoary turmeric oil has antiviral, antibacterial and antiinflammatory effects, and can be used for treating common cold, cholera, emesis, diarrhea, summer-heat syndrome, apoplexy with symptoms of phlegm retention, traumatic injury, qi loss, headache, toothache due to pathogenic wind-fire, bronchial asthma, cough, abdominal pain due to cold and heat, lumbago, backache, pruritus, scabies, undefined lump, traumatic injury, scald, burn, snake worm, Carnis Canitis, Scolopendra wound, hematemesis, insomnia, and traumatic hemorrhage. The clove oil has the effects of resisting and inhibiting bacteria, killing fungi in the air and the like.
After the five volatile oils are compatible, the components interact with each other, the blumea oil and the zedoary turmeric oil with obvious antibacterial activity are taken as main components, and the patchouli oil and the vitex oil are matched to jointly kill or inhibit common pathogenic bacteria and viruses in the air, thereby playing a role in synergy.
As a preferred embodiment, in the present invention, the mass ratio of the patchouli oil, the catmint oil, the clove oil, the blumea oil and the zedoary oil in the traditional Chinese medicine composition is preferably 1-4:1-4:1-4:5-10:5-10, and more preferably 10:10:2:1: 4.
The invention also provides a traditional Chinese medicine air freshening disinfectant, which comprises the traditional Chinese medicine composition and a substrate; as an implementable technical scheme, the mass ratio of the traditional Chinese medicine composition to the matrix is 1-100: 0-99, and preferably 1:49 or 1: 9.
The traditional Chinese medicine air freshening disinfectant is obtained by mixing the traditional Chinese medicine composition according to a certain proportion and adding the mixture into the matrix, the mixing mode of the traditional Chinese medicine composition is not specially limited, and the mixing modes of a three-tone method, a four-mode method and the like in the prior art can be used for mixing the traditional Chinese medicine volatile oil.
The type of substrate is not particularly limited in the present invention, and any known air freshening disinfectant substrate may be used in the present invention. As a preferred embodiment, the matrix of the invention comprises absolute ethanol.
The invention also provides application of the traditional Chinese medicine air freshening disinfectant in killing and/or inhibiting microorganisms.
Preferably, the microorganism of the present invention includes staphylococcus aureus, escherichia coli, staphylococcus albus, and influenza a virus. Further preferably, the influenza a virus is influenza a virus H5N 9.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
In the examples of the present invention, NIH (National Institute of health, USA) was a white mouse (certification number: 0027327), purchased from the center of laboratory animals in Guangdong province.
In the present invention, all the raw material components are commercially available products well known to those skilled in the art unless otherwise specified.
Example 1
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 7.5:5:1:1: 1.
Example 2
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 10:5:4:2: 2.
Example 3
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 10:7.5:1:4: 1.
Example 4
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 10:10:2:1:4 to obtain the composition.
Example 5
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 7.5:5:2:4: 4.
Example 6
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 7.5:7.5:4:1:2 to obtain the composition.
Example 7
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 7.5:10:1:2: 1.
Example 8
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 5:5:1:1: 1.
Example 9
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 5:7.5:2:2: 2.
Example 10
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 5:10:4:4: 4.
Example 11
Preparation of Chinese medicinal composition
Mixing the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the fineleaf schizonepeta oil according to the mass ratio of 10:10:4:4: 4.
Example 12
Bacteriostatic action (bacteriostasis ring method) of single and compound Chinese medicine volatile oil
1.1 Experimental materials
Sample preparation:
sample 1 moxa leaf oil
Sample 2: zedoary turmeric oil
Sample 3: patchouli oil
Sample 4: schizonepeta oil
Sample 5: clove oil
Sample 6: example 1 the prepared Chinese medicinal composition
Experimental strains:
staphylococcus aureus, Escherichia coli, Staphylococcus albus)
1.2 Experimental methods
Respectively and uniformly coating the liquid culture of the three bacteria for 16-18 hours on the surface of a common plate by using sterile cotton swabs, slightly drying, clamping drug sensitive paper sheets with quantitative sample liquid medicine by using sterile tweezers, flatly paving the drug sensitive paper sheets on the surface of the plate inoculated with the bacteria, placing 3 drug sensitive paper sheets on one plate, culturing at 37 ℃, and observing the result the next day.
1.3 analysis of results
Table 1 shows the bacteriostatic effect of the single herb essential oil (diameter of bacteriostatic ring, mm, n is 2-5,)
note that the comprehensive bacteriostatic index, D is A × 0.3+ B × 0.2.2 + C × 0.5.5, the same below.
n represents the number of experimental repetitions (the same applies below).
As can be seen from Table 1, the bacteriostatic index of the traditional Chinese medicine composition is obviously higher than that of the volatile oil of a single traditional Chinese medicine, which shows that the compatibility application of the volatile oil of the traditional Chinese medicine can improve the bacteriostatic effect.
Example 13
Bacteriostasis (bacteriostasis ring method) of compound traditional Chinese medicine volatile oil with different proportions
2.1 materials of the experiment
Sample preparation:
samples 7-15 are in turn the Chinese medicinal composition prepared in examples 2-10 of the present invention
Experimental strains: same as in item 1.1 of example 12
2.2 Experimental methods
Same as example 12 in 1.2
2.3 analysis of results
Table 2 shows the bacteriostatic effect of the compound Chinese medicinal essential oil (diameter of bacteriostatic ring, mm, n is 2-5,)
the above experiment is the result obtained by orthogonal design according to different proportions of the volatile oil, and as can be seen from the above table, the 9 formulas all have antibacterial effects, which indicates that the blumea oil, zedoary turmeric oil, patchouli oil, clove oil and catnip oil applied in the invention generate synergistic effect in the antibacterial aspect through compatibility.
Example 14
Bacteriostasis of compound traditional Chinese medicine volatile oil with different proportions (test tube dilution method)
3.1 Experimental materials
Sample preparation:
sample 6 is the traditional Chinese medicine composition prepared in example 1 of the present invention;
sample 9 is the traditional Chinese medicine composition prepared in example 4 of the present invention;
sample 16 is the traditional Chinese medicine composition prepared in example 11 of the present invention.
Experimental strains: same as in item 1.1 of example 12
3.2 Experimental methods
Respectively adding 1m L Tween 80 into 3m L samples 6, 9 and 16 to dissolve, adding nutrient broth to prepare a product containing 0.30m L1, 0.15m L2, 0.075m L, 0.0375m L, 0.01875m L, 0.009375m L, 0.004688m L, 0.002344m L and 0.001172m L of Chinese medicinal volatile oil per m L0, diluting the samples with 9 medicinal concentrations, wherein the total amount of each tube is 1m L, and thermally sterilizing.
And (4) control: the strain control is culture medium without drug and test bacteria, and the drug control is liquid medicine without test bacteria.
The concentration of each concentration tube and strain control tube of each row of liquid medicine is 106cfu·ml-10.1m L, culturing at 37 deg.C for 24 hr, inoculating one ring of each concentration with inoculating loop, culturing at 37 deg.C for 24 hr, observing the result, observing the aseptic growth of each tube with turbidity as index, and observing the aseptic colony growth of each plate with naked eye.
The Minimum Inhibitory Concentration (MIC) is determined and refers to the minimum drug concentration contained in the test strain that completely inhibits growth.
The Minimum Bactericidal Concentration (MBC) was determined, which refers to the minimum drug concentration that completely kills the growth of the test strain.
3.3 analysis of results
TABLE 3 MIC and MBC determination results of compound Chinese medicinal volatile oil at different ratios for 3 bacteria (μ L m L)-1)
MIC and MBC are two important indexes of bacteriostasis and sterilization of the medicine, and the smaller the value is, the better the antibacterial effect of the medicine is. The results show that: the samples 9 and 16 have good comprehensive sterilization effect and good comprehensive bacteriostasis effect.
Example 15
Antiviral effect of compound Chinese medicinal volatile oil with different proportions
4.1 Experimental materials
Sample preparation:
sample 6 is the traditional Chinese medicine composition prepared in example 1 of the present invention;
sample 9 is the traditional Chinese medicine composition prepared in example 4 of the present invention;
sample 16 is the traditional Chinese medicine composition prepared in example 11 of the present invention.
The positive control drug ribavirin, lot 1901016, was purchased at the Guangzhou drug laboratory.
And (3) poisoning seeds: influenza A virus H5N9 strain was passaged to MDCK Canine kidney cells (Mad in-Darby Canine Kidney cell, Ma-Dou's Canine Kidney cell), and its titer was judged by lesion and hemagglutination of chicken erythrocytes at 37 ℃ with 5% CO2And culturing in an incubator.
4.2 Experimental methods
The virus test was carried out on 96-well microplate with 0.1m L per well and 1x10 cell number, according to the requirements of the guidelines for the study of new drugs in Chinese medicine (the Ministry of health and administration of medicine of the people's republic of China, 1994 edition)5After each m L grows into a single layer, diluting the traditional Chinese medicine into 4 different concentrations by using a maintenance solution, discarding the original culture solution, adding the traditional Chinese medicine samples with different concentrations into cell holes, wherein each hole is 0.1m L, and the minimum dilution at which the cells do not have lesions is taken as the non-toxic limit of the liquid medicine to the cells50(50% tissue culture infectious dose). The test is provided with a virus control, a cell control, a drug toxicity control and a positive drug control (ribavirin). Standing at 37 deg.C for 5% CO2And culturing in an incubator. Lesions were observed under an inverted microscope and recorded daily. The observation was continued for 7 days and terminated when the cells in the virus control wells showed +++ lesions. The extent of lesion appearance of the cells was recorded on a 6-point scale: "-": the cells grow normally and no disease is generated; "+/-": cytopathic effects are less than 10% of the whole monolayer of cells; "+": cytopathic effects are less than 25% of the whole monolayer of cells; "++": cytopathic effects are less than 50% of the whole monolayer of cells; "+++": cytopathic effects are less than 75% of the whole monolayer of cells; "++++": cytopathic effects account for more than 75% of the entire monolayer of cells.
4.3 analysis of results
TABLE 4 inhibition of influenza A virus H5N9 by different mixture ratio of Chinese medicinal volatile oil
Note: the "+" completely inhibits the virus "-" has no inhibiting effect on the virus
The results show that the virus TCID is infected50At 100 doses, ribavirin is 100 μ g m L-1Has obvious inhibiting effect on H5N9, and sample No. 9 has 150 mu L m L under the condition of sub-toxic concentration-1Has certain inhibiting effect on H5N 9. It is shown that sample 9 has the effect of inhibiting common viruses in the air, and has the air sterilization effect.
Example 16
Preparation of traditional Chinese medicine air freshening disinfectant
And (3) uniformly mixing the traditional Chinese medicine composition prepared in the embodiment 4 with absolute ethyl alcohol according to the mass ratio of 1: 9.
Example 17
Preparation of traditional Chinese medicine air freshening disinfectant
And (3) uniformly mixing the traditional Chinese medicine composition prepared in the embodiment 4 with absolute ethyl alcohol according to the mass ratio of 1: 49.
Example 18
Simulation of field experiments
5.1 Experimental materials
Sample preparation:
traditional Chinese medicine air freshening disinfectant 1: the traditional Chinese medicine air freshening disinfectant prepared in the embodiment 16 of the invention;
test strains: white staphylococcus, the strain generation number is 6-8, and a strain suspension is prepared by using common broth.
More than 90% of particles phi of the aerosol generator are less than or equal to 5 mu m.
The volume of the aerosol chamber is 10m3。
A sampler: an FA-1 type impact air microorganism sampler.
5.2 Experimental methods
The inspection basis is as follows: ministry of health, Disinfection Specification (2002 edition) 2.1.3.
The test temperature is 23-24 ℃, and the relative humidity is 50-58%.
The flow rate of the bacteria-contaminated sprayer is 21.7L/min, the spraying time is 5min, the stirring is 5min, and the standing is 5 min.
Disinfection method, 2m L/m is used in test3The amount of the extract is measured by spraying the sample to a laboratory by using a QPQ-2300 electric aerosol sprayer, and sampling after sterilizing for 60 min. The control group was treated with deionized water instead of the disinfectant, and the other operations were the same as those of the test group. The experiment was repeated 3 times.
Adopting an FA-1 impact type air microorganism sampler, wherein the sampling flow is 28.3L/min, and a point is arranged at the center of a room and 1.0m away from the ground for sampling, the sampling is carried out when the disinfection action time is 0min and 60min, the sampling time of a control group sampler is 5s and 5s respectively, the sampling time of a test group sampler is 5s and 1min respectively, the number of viable bacteria stored at different times is measured after sampling, and the killing rate is calculated according to the following formula:
Nt: the natural rate of bacterial death in the air;
V0' and Vt: air bacteria content before the start of the control group test and in different time in the test process are respectively;
Kt: the sterilization treatment has the effect of killing bacteria in the air;
V0' and Vt': the air bacterial contents before the sterilization treatment and during the sterilization process of the test groups at different times are respectively shown.
5.3 analysis of results
TABLE 5 killing effect of Chinese medicinal air-freshening disinfectant 1 on staphylococcus albus
Negative control: both the diluent and the medium grow aseptically.
The traditional Chinese medicine air freshening disinfectant 1 can kill common bacteria in the air and has an air sterilization effect.
Example 19
In situ experiment
6.1 Experimental materials and methods
Sample preparation:
traditional Chinese medicine air freshening disinfectant 2: the traditional Chinese medicine air freshening disinfectant prepared in the embodiment 16 of the invention.
Traditional Chinese medicine air freshening disinfectant 3: the traditional Chinese medicine air freshening disinfectant prepared in embodiment 17 of the invention.
Test environment and instrumentation: room volume: 70m3
Temperature and humidity: room temperature: 31 ℃, room relative humidity: 85 percent of
Spraying equipment: electric aerosol atomizer, model: DQP-600, manufacturer: beijing Songyuan Huaxing science and technology development Co., Ltd
Disinfecting and sampling:
the sampling is carried out for 2 times in total, and the sampling time points are 0h and 2h respectively.
The sampling method comprises the following steps: two plates with a diameter of 9cm were placed at each of the 5 sampling points, the sampling height was 1.0m, the petri dish was opened, after exposure for 10 minutes, the lid was closed, the sample was incubated at 37 ℃ for 24 hours, the number of colonies was counted and the mortality was calculated according to the following formula.
Sterilizing at a dosage of 1m L per cubic meter by uniformly spraying onto room with an electric aerosol sprayer, and sterilizing for 2 hr.
6.3 analysis of results
TABLE 6 disinfectant effect of Chinese medicinal air-freshening disinfectant 2 in closed state on site
TABLE 7 disinfectant effect of Chinese medicinal air-freshening disinfectant 3 in closed state on site
The results show that the traditional Chinese medicine air freshening disinfectant 2 and the traditional Chinese medicine air freshening disinfectant 3 can kill bacteria in the air and can effectively reduce the number of bacteria in the air.
Example 20
Acute oral toxicity test
7.1 Experimental materials
Sample preparation:
traditional Chinese medicine air freshening disinfectant 2: the traditional Chinese medicine air freshening disinfectant prepared in the embodiment 16 of the invention;
traditional Chinese medicine air freshening disinfectant 3: the traditional Chinese medicine air freshening disinfectant prepared in embodiment 17 of the invention.
Test animals: NIH (national Institute of health) is a white mouse, which is half male and female, and has a body weight of 18-22 g.
7.2 Experimental methods
Taking 20 NIH-series mice with half male and female parts and weight of 18-22 g, adaptively feeding the animals for 2D, fasting the animals overnight and freely drinking water before administration, diluting 5.0287g of the traditional Chinese medicine air refreshing disinfectant 2 and 3-20 m of the traditional Chinese medicine air refreshing disinfectant 3-20 m L by using a proper amount of peanut oil during test, performing intragastric lavage once according to the weight of 20m L/kg, namely the dose of 5000mg/kg, immediately observing the animal reaction, continuously observing for two weeks, recording the toxic reaction condition and death distribution of the animals, simultaneously performing autopsy on the dead animals, recording the pathological change condition, and judging L D50Whether it is large or notAt 5000mg/kg body weight.
7.3 analysis of results
The test result shows that: the traditional Chinese medicine air freshening disinfectant 2 and the traditional Chinese medicine air freshening disinfectant 3 are subjected to intragastric administration once according to the dose of 5000mg/kg body weight, and no obvious toxic reaction occurs to animals. After administration, the animal reaction is mainly manifested by reduced activity, and excitation and jumping of a few animals; the animal activity substantially recovered to normal 2 hours after dosing; no animals died.
Therefore, it was determined to be L D50Greater than 5000mg/kg body weight.
According to the toxicity evaluation criteria in the "Disinfection technical Specification" (2002 edition) of the Ministry of public health of the people's republic of China:
LD50the people with the weight more than 5000mg/kg are actually nontoxic;
LD50the patients with 501 mg/kg-5000 mg/kg body weight belong to low toxicity;
LD50the body weight of 51 mg/kg-500 mg/kg belongs to moderate toxicity;
LD50the patients with 1 mg/kg-50 mg/kg body weight belong to high toxicity;
LD50those with the weight less than 1mg/kg are extremely toxic.
Because the traditional Chinese medicine air refreshing disinfectant 2 and the L D of the traditional Chinese medicine air refreshing disinfectant 3 are adopted50More than 5000mg/kg body weight, the traditional Chinese medicine air refreshing disinfectant 2 and the traditional Chinese medicine air refreshing disinfectant 3 are considered to belong to nontoxic products.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A Chinese medicinal composition is characterized by comprising patchouli oil, catnip oil, clove oil, blumea oil and zedoary turmeric oil.
2. The traditional Chinese medicine composition as claimed in claim 1, wherein the mass ratio of the patchouli oil, the catmint oil, the clove oil, the blumea oil and the zedoary turmeric oil is 1-4:1-4:1-4:5-10: 5-10.
3. The traditional Chinese medicine composition according to claim 2, wherein the mass ratio of the blumea oil, the zedoary turmeric oil, the patchouli oil, the clove oil and the catnip oil is 10:10:2:1: 4.
4. A traditional Chinese medicine air freshening disinfectant, which is characterized by comprising the traditional Chinese medicine composition as claimed in any one of claims 1-3 and a substrate; the mass ratio of the traditional Chinese medicine composition to the matrix is 1-100: 0 to 99.
5. The traditional Chinese medicine air freshening disinfectant as claimed in claim 4, wherein the mass ratio of the traditional Chinese medicine composition to the base material is 1: 9.
6. The traditional Chinese medicine air freshening disinfectant as claimed in claim 4, wherein the mass ratio of the traditional Chinese medicine composition to the base is 1: 49.
7. The traditional Chinese medicine air freshening disinfectant of any one of claims 4-6 in which said substrate comprises anhydrous ethanol.
8. Use of a traditional Chinese medicine air freshening disinfectant as claimed in any one of claims 4 to 7 in killing and/or inhibiting microorganisms.
9. Use according to claim 8, wherein the microorganisms comprise Staphylococcus aureus, Escherichia coli, Staphylococcus albus and influenza A virus.
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