CN1703959A - Preparation capable of killing virus and bacteria and its preparation process - Google Patents
Preparation capable of killing virus and bacteria and its preparation process Download PDFInfo
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- CN1703959A CN1703959A CN 200410042663 CN200410042663A CN1703959A CN 1703959 A CN1703959 A CN 1703959A CN 200410042663 CN200410042663 CN 200410042663 CN 200410042663 A CN200410042663 A CN 200410042663A CN 1703959 A CN1703959 A CN 1703959A
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Abstract
The invention relates to a preparation for eradicating viruses, which contains natural plant essential oil selected from one or more of the following, eucalyptus oil, tea plant oil, valerian oil and sandalwood oil, which have eradication action to viruses. The preparation may also comprises active ingredients extracted from the essential oil as the reactive composition for eradicating viruses.
Description
Technical field
The present invention relates to preparation of a kind of germ capable of killing virus and preparation method thereof, especially a kind of air purifying preparation and preparation method thereof.
Technical background
The room air pollution problem is serious day by day since the time in all one's life nearly 70% of people spend indoor, so room air is extremely important to health effects.According to statistics, nearly half the population in the whole world is in the room air pollution, and room air pollution has caused 35.7% respiratory disease, 22% chronic pneumonia and 15% tracheitis, bronchitis and lung cancer.The World Health Organization clearly classifies room air pollution and hypertension, acquired immune deficiency syndrome (AIDS) etc. as ten of human health and threatens greatly.Along with the outburst of global influenza great outburst and SARS epidemic in 2003 in recent years, make that microbiological pollution becomes the problem that people more and more pay close attention in the room air.Up to now, the known virus that can cause respiratory tract infection just has 200 kinds more than, and the generation overwhelming majority of these infection is that room air is propagated.Solve biotic pollution problem in the room air, become the important means that people pursue healthy body.
Along with the attention to indoor air environment, people have begun to adopt the whole bag of tricks that room air is purified.At present, existing on the market air purifier miscellaneous, most air purifier are to adopt filtration, ultraviolet or photocatalysis technology etc. that room air is carried out sterilization.It is low that these equipment exist fungicidal effectiveness, and equipment component can not man-machine coenecium, uses shortcomings such as inconvenience.The research report of " being installed in the ultraviolet germicidal influence healthy to the staff---the many cross matchings of double blinding of office's ventilating system " also delivered one piece and has been entitled as by " lancet " of Britain (LANCET) magazine in the 362nd phase in November, 2003.Reported in the defrost pan and cooling coil of air conditioner ventilating system, the ill problem that ultraviolet germicidal can significantly reduce the deskman has been installed.Ultraviolet germicidal has been installed in this research in the defrost pan of air conditioner ventilating system and cooling coil makes the bacteria containing amount in the return air reduce, but it is bigger to adopt this method to carry out its up-front investment of air cleaning, and, will cause the incompatibility of people to the ozone smell because the ozone that ultraviolet-sterilization produces can be brought back in the office.
There are some patent applications to disclose various air purifying preparations.Chinese patent application 90100576.2 discloses a kind of air purifying preparation, is made by agar, Du's essential oil, essence, Tween 80 or triton x-100 and pigment, and the sterilization mycostatic nature is arranged.Chinese patent 92105506.4 discloses a kind of air purifying preparation that bactericidal effect is arranged, and is to add the second alcohol and water to make in the lactic acid of biofermentation production, also can contain essence.Chinese patent application 94102199.8 discloses a kind of air purifying preparation, and it contains deionized water, edible ethanol, Chinese herbal medicine water decoction, isopropyl myristate, dispersant and essence; Wherein the Chinese herbal medicine water decoction is the water decoction that borneol, the root of Dahurain angelica, frutus cnidii are arranged.Chinese patent application 96116958.3 discloses the air purifying preparation that a kind of heat is released, it mainly is made up of by certain proportioning honeysuckle flower, chrysanthemum, Chinese arborvitae shell, dried peppermint leaf, styrax, borneol, Moschus, storax, rhizoma atractylodis, tarragon, folium isatidis, rose, sandalwood, eupatorium, grass-leaved sweetflag etc., capable of purifying air, resuscitation with aromatics, arousing brain, raising spirit, eliminating inflammation and expelling toxin has prevention and supplemental treatment effect to diseases such as cerebrovascular disease and virus flus.Chinese patent application 02115757.X discloses the aerosol air disinfectant by iodine, bromine, polyvinylpyrrolidone and solvent composition, can insect killing effect be arranged to bacteriums such as Escherichia coli, staphylococcus aureus, withered grass bacterium black mutation brood cells.But do not see the air purifying preparation safely and effectively that makes by natural material that viral germs such as influenza first type virus, influenza B virus is had killing effect, do not see existing kill virus germ effect yet, the air purifying preparation of health-care effect is arranged again.
The preparation that generally is used for the kill virus bacterium as disinfectant, contains the chemical substance that can kill, for example: 01110639.5 more, 00119549.2,01109127.4,00106205.0,98114208.7,95114242.9,92106255.9,90104800.3,90101240.3.The material of these chemosynthesis is made the kill virus bacteria preparation, has certain toxic and side effect, and permanent the use is especially as air sterillization, purification preparation, unfavorable to health.Chinese medicine antiseptic is many to be made by the plurality of Chinese composition, for example contains traditional Chinese medicine ingredients such as sweet tangerine, rheum officinale, tarragon, cloves in 01144571.8; 97108166.2 in contain traditional Chinese medicine ingredients such as frutus cnidii, folium eucalypti, kuh-seng, mother chrysanthemum; 92107142.6 in contain compositions such as rhizoma homalonemae, the Radix Astragali, Radix Angelicae Sinensis, the root of Dahurain angelica.For a long time, people mainly are used as plants essential oil spices and utilize its special health care to do " aromatotherapy "---promptly popular at present scent.Do not see the preparation of the germ capable of killing virus that makes by natural plant essential oils as yet.
Summary of the invention
In order to overcome the deficiency of prior art, the object of the invention is a kind of preparation that influenza first type virus, influenza B virus, Escherichia coli, staphylococcus aureus, shigella dysenteriae, black-koji mould and/or saccharomyces albicans etc. is had killing effect.Said preparation is especially suitable for use as air purifying preparation, purifies the air of a room, and makes the pure and fresh cleaning of room air, and the health care of tranquilizing and refreshing, preventing cold and relieving cough, pharynx-clearing throat-benefiting and improvement sleep is provided.
Another object of the present invention provides the preparation method of the preparation of a kind of germ capable of killing virus.
The inventor explores through a large amount of deep researchs, find that adopting natural plant essential oils is raw material, adopting suitable prescription can prepare safety has no side effect, the preparation that influenza first type virus, influenza B virus, Escherichia coli, staphylococcus aureus, shigella dysenteriae, black-koji mould and/or saccharomyces albicans etc. is had killing effect, therefore, be especially suitable for use as the preparation of air purifying preparation or other sterilizations or the composition of product or its kill virus germ.
The invention provides the preparation of a kind of germ capable of killing virus, contain the active component of natural plant essential oils as germ capable of killing virus.Described natural plant essential oils can be selected from one group that by eucalyptus oil, tea oil, valerian oil, sandalwood oil and other viral germ is made up of the natural plant essential oils of killing action one or more natural plant essential oils or by the active ingredient of its extraction.
The invention provides the preparation of a kind of germ capable of killing virus, contain two or more natural plant essential oils of being selected from one group that by eucalyptus oil, tea oil, valerian oil, sandalwood oil and other viral germ is made up of the natural plant essential oils of killing action or by the active ingredient of its extraction.
The invention provides the preparation of a kind of germ capable of killing virus, contain two or more the plants essential oil that is selected from a group that forms by eucalyptus oil, tea oil, valerian oil and sandalwood oil, or by the active ingredient of its extraction.
Described plants essential oil can be the essential oil by plant extract, also can be the commercially available prod.Described method by the plant extract essential oil can be conventional or known method according to this area.
Described eucalyptus oil can be the essential oil that is extracted by eucalyptus leaves, also can be the commercially available prod.Described method by eucalyptus leaves extraction essential oil can be conventional or known method according to this area.
Described tea oil can be the essential oil that is extracted by narrow leaved tea tree, also can be the commercially available prod.Described method by narrow leaved tea tree extraction essential oil can be conventional or known method according to this area.
Described valerian oil can be the essential oil that is extracted by valerian, also can be the commercially available prod.Described method by valerian extraction essential oil can be conventional or known method according to this area.
Described sandalwood oil can be the essential oil that is extracted by sandalwood, also can be the commercially available prod.Described method by sandalwood extraction essential oil can be conventional or known method according to this area.
The described active ingredient of extracting by described plants essential oil, comprise by extractible one or more active ingredients in these plants essential oils, conventional or known active ingredient as those, for example: 1 in the eucalyptus oil, the 8-Cineole, 4-terpineol in the tea oil, the α-santalol in the sandalwood oil, β-santalol etc.
The active constituent content that contains in the described plants essential oil can be the content of effective that contains usually in the plants essential oil buied of the plants essential oil for preparing according to this area routine or known method or market, for example, eucalyptus oil contains 60～80% (mass percents) 1, the 8-Cineole, tea oil contains 30～35% 4-terpineols, sandalwood oil contains 10～25% α-santalol and 10～25% β-santalol, and valerian oil contains 25～35% Bronyl acetates.
When preparation of the present invention contained two or more described plants essential oil, the content of described two or more described plants essential oil can be respectively to be 0.05～15 part.
When preparation of the present invention contains two or more described plants essential oil, wherein a kind of preferred eucalyptus oil or tea oil or valerian oil.The content of described eucalyptus oil or tea oil can be 5～15 parts, and the content of other plant essential oil is 10～0.05 parts.The content of described valerian oil can be 1～10 part.
Preparation of the present invention can contain 5～0.05 parts sandalwood oil, and 5～15 parts eucalyptus oil and/or 5～15 parts tea oil and/or 1～10 part valerian oil.
Can also contain auxiliary material and/or solvent in the preparation of the present invention.Described auxiliary material can be the conventional or known auxiliary material in this area of the needs in order to make preparation of the present invention adapt to various different purposes, for example, and emulsifier.Described solvent can be the conventional or known solvent in this area, for example, and water.When preparation of the present invention contained auxiliary material and/or solvent, the content of described auxiliary material and/or solvent was the conventional or known content in this area.Preparation of the present invention also can be dissolved in before use in this area routine or the known solvent and using.
When needs made water as solvent, preparation of the present invention also comprised emulsifier.
When preparation of the present invention contained emulsifier, described emulsifier was the conventional or known emulsifier in this area, for example, and Tween 80 and/or sorbester p17.Emulsifier of the present invention can contain 0～100% (heavy %) Tween 80 and 100%～0% sorbester p17, preferred 50%～95% Tween 80 and 50%～5% sorbester p17.
The content of described emulsifier is the conventional or known content in this area.Preferably contain 7～4 parts emulsifier in the preparation of the present invention, and 4～8 parts plants essential oil.
A kind of method for preparing the preparation of germ capable of killing virus provided by the invention can may further comprise the steps: (1) obtains plants essential oil, and mix (1) step gained plants essential oil (2) with auxiliary material.
Described plants essential oil can be one or more plants essential oil, and it comprises that eucalyptus oil, tea oil, valerian oil, sandalwood oil and/or other have the natural plant essential oils of killing action to viral germ.Described plants essential oil can pass through by plant extract, or commercially available approach obtains.The method of described extraction can be the conventional or known method of this area.When plants essential oil contained more than one plants essential oil, described plants essential oil can mix acquisition by any order or according to the described mode of following paragraph by more than one plants essential oils.Described mixing can be carried out according to the conventional or known method of this area.
Mixing described in (2) step can be the conventional or known method of this area.Described mixing can be that the mixture with one or more plants essential oil mixes with auxiliary material; Also can be that a kind of and auxiliary material in the plants essential oil is mixed, again with in the mixture that obtained and other several plant essential oils or other several plant essential oils one or more or mix.The order by merging of the mixing of more than one plants essential oil, one or more plants essential oils and auxiliary material can be any order.The mode of described mixing can be the conventional or known mode of this area.
Described auxiliary material can be an emulsifier.Described emulsifier can be conventional or known emulsifier, for example Tween 80 and/or a sorbester p17 of this area.
Preparation of the present invention is especially suitable for use as air purifying preparation.
Preparation of the present invention has the effect of significantly killing influenza first type virus, influenza B virus, Escherichia coli, staphylococcus aureus, shigella dysenteriae, black-koji mould and/or saccharomyces albicans; And multiple virus, multiple germ in the air had remarkable killing action.Preparation of the present invention is especially suitable for use as air purifying preparation, can purify the air of a room, and makes the pure and fresh cleaning of room air, and the indoor air environment of a health is provided, and has the health care of tranquilizing and refreshing, preventing cold and relieving cough, pharynx-clearing throat-benefiting and improvement sleep concurrently.
By preparation of the present invention being distributed to airborne device, equipment or machine, use preparation of the present invention, especially the preparation of the present invention that contains emulsifier is as air purifying preparation, can good killing action be arranged to airborne viral germ, also have the health care of certain tranquilizing and refreshing, preventing cold and relieving cough, pharynx-clearing throat-benefiting and improvement sleep simultaneously.Preparation of the present invention can be dispersed in the use of airborne device, equipment or machine, can make the clean-up effect of air better than preparation of the present invention being positioned over the clean-up effect that volatilizees naturally in the air.But be not precluded within some special environment, use preparation of the present invention to have better effect by other modes.
Raw materials used among the present invention is natural plant essential oils with health role, for example eucalyptus oil, tea oil, valerian oil and sandalwood oil.Find that after deliberation eucalyptus oil can resist the infection of bacterium, fungi, virus, staphylococcus aureus, hemolytic streptococcus, dysentery characterized by blood in the stool bacillus, typhoid bacillus, Escherichia coli, Pseudomonas aeruginosa etc. are all had inhibitory action external; Cineole in the eucalyptus oil has the function of relievining asthma; Eucalyptus oil also is usually used in treating hypoglycemia, scald, mucositis, flu, laryngitis, asthma, rheumatism etc.Finding after deliberation to contain in the tea oil multiplely has inhibiting natural component to microorganism, terpinenol-the 4th wherein, and antibiotic main matter, it has antibiotic vigor to comprising Candida albicans, Escherichia coli, golden yellow grape bacillus, pseudomonas aeruginosa etc.; Terpinenol, alpha-terpineol, australene also have inhibitory action to Staphylococcus epidermidis and Propionibacterium.Find that after deliberation valerian oil also has antibacterial action, especially Gram-positive bacteria is renderd a service better; Other effects comprise acceleration bodies intravascular coagulation process, antidiuresis, anti-oxidant, antifatigue effect; Use valerian oil can improve bronchitis, asthma, laryngitis, stable mood, anti-dejected, hypotensive and Cure for insomnia.That sandalwood oil then often is used to treat is tired, the usefulness of bronchitis, urinary tract infection and impotence.The present invention selects suitable plants essential oil by further investigation, according to its antibacterial activity and health-care effect, prepares the preparation of the present invention of fine kill virus germ effect according to proper formula.
Advantage of the present invention and good effect are: it is raw material that the present invention selects natural plant essential oils with health role for use, natural having no side effect; Show after deliberation, use the present invention can significantly kill influenza first type virus, influenza B virus, Escherichia coli, staphylococcus aureus, shigella dysenteriae, black-koji mould and/or saccharomyces albicans etc.; Particularly multiple virus and germ in the air had remarkable killing action.The present invention can solve the microbial contamination of room air, purifies the air of a room, and makes the pure and fresh cleaning of room air, for you provide the indoor air environment of a health, can also provide plants essential oil special health care.
Embodiment
Below will the invention will be further described by embodiment, these descriptions are not that content of the present invention is done further to limit.One skilled in the art will understand that to be equal to replacement to what technical characterictic of the present invention was done, or corresponding the improvement, still belong within protection scope of the present invention.
Embodiment 1
Through overtesting, preparation of the present invention can use the emulsifier of following prescription.
Table 1: emulsifier ratio
| The emulsifier numbering | ????1 | ????2 | ????3 | ????4 | ????5 | ????6 | ????7 | ????8 |
| Tween 80 (umber) | ????0 | ????1 | ????1 | ????2 | ????1 | ????4 | ????8 | ????10 |
| Sorbester p17 (umber) | ????1 | ????0 | ????1 | ????1 | ????2 | ????1 | ????1 | ????1 |
| The emulsifier numbering | ????9 | ????10 | ????11 | ????12 | ????13 | ????14 | ????15 | ????16 |
| Polysorbate60 (umber) | ????0 | ????1 | ????1 | ????2 | ????1 | ????4 | ????8 | ????10 |
| Sorbester p18 (umber) | ????1 | ????0 | ????1 | ????1 | ????2 | ????1 | ????1 | ????1 |
Embodiment 2
(the fragrant biochemical technology in sky, Kunming Co., Ltd produces with eucalyptus oil, contain 1,8-Cineole 65%), tea oil (plant Co., Ltd and produce by Nanning ten thousand tame brightness, contain 4-terpineol 30%) and sandalwood oil (the fragrant biochemical technology in sky, Kunming Co., Ltd production, contain α-santalol 12%, β-santalol 15%) the mixed preparation A1 to A18 of the present invention that gets following prescription.
Table 2: prepare preparation of the present invention
| The raw material umber | ||||||||||||||||||
| Preparation of the present invention | ??A1 | ??A2 | ??A3 | ??A4 | ??A5 | ??A6 | ??A7 | ??A8 | ??A9 | ??A10 | ??A11 | ??A12 | ??A13 | ??A14 | ??A15 | ??A16 | ??A17 | ??A18 |
| Eucalyptus oil | ??8 | ??8 | ??8 | ??8 | ??8 | ??8 | ??12 | ??6 | ??6 | ??12 | ??6 | ??6 | ??16 | ??8 | ??8 | ??16 | ??8 | ??8 |
| Tea oil | ??8 | ??12 | ??16 | ??8 | ??12 | ??16 | ??8 | ??6 | ??8 | ??8 | ??6 | ??8 | ??8 | ??6 | ??8 | ??8 | ??6 | ??8 |
| Sandalwood oil | ??0.4 | ??0.4 | ??0.4 | ??0.8 | ??0.8 | ??0.8 | ??0.4 | ??0.2 | ??0.2 | ??0.8 | ??0.4 | ??0.4 | ??0.4 | ??0.2 | ??0.2 | ??0.8 | ??0.4 | ??2.0 |
Embodiment 3
With eucalyptus oil (containing 1,8-Cineole 79%) and the mixed preparation B1 to B18 of the present invention that gets following prescription of sandalwood oil (containing α-santalol 20%, β-santalol 11%).
Table 3: prepare preparation of the present invention
| The raw material umber | ||||||||||||||||||
| Preparation of the present invention | ??B1 | ??B2 | ??B3 | ??B4 | ??B5 | ??B6 | ??B7 | ??B8 | ??B9 | ??B10 | ??B11 | ??B12 | ??B13 | ??B14 | ??B15 | ??B16 | ??B17 | ??B18 |
| Eucalyptus oil | ??5 | ??6 | ??8 | ??10 | ??12 | ??14 | ??8 | ??6 | ??8 | ??10 | ??12 | ??14 | ??8 | ??6 | ??8 | ??10 | ??12 | ??14 |
| Sandalwood oil | ??4.2 | ??0.1 | ??0.1 | ??0.1 | ??0.1 | ??0.1 | ??0.4 | ??0.2 | ??0.2 | ??0.2 | ??1.5 | ??0.2 | ??0.6 | ??0.6 | ??0.3 | ??0.3 | ??4.5 | ??3.0 |
Embodiment 4
With tea oil (containing 4-terpineol 35%) and sandalwood oil (containing α-santalol 25%, β-santalol 25%) mixed the formulation C of the present invention 1 of following prescription to C18.
Table 4: prepare preparation of the present invention
| The raw material umber | ||||||||||||||||||
| Preparation of the present invention | ????C1 | ????C2 | ????C3 | ????C4 | ????C5 | ????C6 | ????C7 | ????C8 | ????C9 | ????C10 | ????C11 | ????C12 | ????C13 | ????C14 | ????C15 | ????C16 | ????C17 | ????C18 |
| Tea oil | ????5 | ????6 | ????8 | ????10 | ????12 | ????14 | ????5 | ????6 | ????8 | ????10 | ????12 | ????14 | ????5 | ????6 | ????8 | ????10 | ????12 | ????14 |
| Sandalwood oil | ????0.1 | ????0.1 | ????0.1 | ????0.1 | ????0.1 | ????0.1 | ????0.2 | ????0.2 | ????0.6 | ????0.7 | ????0.5 | ????0.2 | ????0.3 | ????0.3 | ????0.3 | ????0.3 | ????0.3 | ????0.3 |
Embodiment 5
Eucalyptus oil (is contained 1,8-Cineole 70%), (Cengong Fine Chemical Plant, Guizhou Prov produces valerian oil, contain Bronyl acetate 25%) and the mixed preparation D1 to D18 of the present invention that gets following prescription of sandalwood oil (containing α-santalol 16%, β-santalol 20%).
Table 5: prepare preparation of the present invention
| The raw material umber | ||||||||||||||||||
| Preparation of the present invention | D1 | ?D2 | ?D3 | ?D4 | ?D5 | ?D6 | ?D7 | ?D8 | ?D9 | ?D10 | ?D11 | ?D12 | ?D13 | ?D14 | ?D15 | ?D16 | ?D17 | ?D18 |
| Eucalyptus oil | 8 | ?8 | ?8 | ?8 | ?8 | ?8 | ?6 | ?6 | ?6 | ?6 | ?6 | ?6 | ?8 | ?8 | ?8 | ?8 | ?10 | ?8 |
| Valerian oil | 12 | ?8 | ?4 | ?12 | ?8 | ?4 | ?6 | ?4 | ?2 | ?6 | ?4 | ?2 | ?6 | ?4 | ?2 | ?6 | ?1 | ?2 |
| Sandalwood oil | 0.4 | ?0.4 | ?0.4 | ?0.8 | ?0.8 | ?0.8 | ?0.2 | ?0.2 | ?0.2 | ?0.4 | ?0.4 | ?0.4 | ?0.2 | ?0.2 | ?0.2 | ?0.4 | ?1.4 | ?0.4 |
Embodiment 6
With the mixed preparation E1 to E6 of the present invention that gets following prescription of eucalyptus oil (containing 1,8-Cineole 75%), valerian oil (containing Bronyl acetate 35%), tea oil (containing 4-terpineol 32%) and sandalwood oil (containing α-santalol 20%, β-santalol 20%).
Table 6: prepare preparation of the present invention
| The raw material umber | ||||||
| Preparation of the present invention | ????E1 | ????E2 | ????E3 | ????E4 | ????E5 | ????E6 |
| Eucalyptus oil | ????5 | ????8 | ????0 | ????8 | ????8 | ????8 |
| Valerian oil | ????0 | ????1 | ????4 | ????8 | ????0 | ????4 |
| Tea oil | ????5 | ????8 | ????6 | ????0 | ????8 | ????0 |
| Sandalwood oil | ????0 | ????0.4 | ????0.05 | ????0.8 | ????2.0 | ????1.8 |
Embodiment 7
The preparation of the present invention that previous embodiment is made makes the preparation F1 to F12 of the present invention of following prescription as the emulsifier that composite plant essential oil and embodiment 1 make.According to proportioning among the embodiment 1 sapn is joined in the tween, stir while add, solution is mixed becomes compound emulsifying agent.Add the composite natral essential oil of prepared preparation of the present invention in the previous embodiment in the compound emulsifying agent according to proportioning in the following table, stir, solution is mixed become settled solution to get final product while add.
Table 7: prepare preparation of the present invention
| Preparation of the present invention | ??F1 | ??F2 | ??F6 | ??F7 | ??F8 | ??F9 | ??F10 | ??F11 | ??F12 |
| Plants essential oil (preparation of the present invention) | ??A10 | ??A14 | ??B14 | ??B3 | ??C11 | ??C16 | ??D5 | ??D9 | ??E1 |
| The emulsifier numbering | ??6 | ??7 | ??3 | ??6 | ??6 | ??4 | ??8 | ??12 | ??8 |
| The umber of composite plant essential oil | ??0.5 | ??0.8 | ??1.0 | ??1.2 | ??1.5 | ??1.8 | ??2.0 | ??2.1 | ??2.5 |
| The compound emulsifying agent umber | ??1 | ??1 | ??1 | ??1 | ??1 | ??1 | ??1 | ??1 | ??1 |
Embodiment 8
An amount of sapn is joined in an amount of tween, stir while add, solution is mixed becomes compound emulsifying agent.The composite natral essential oil A15, B4, C6, the D15 that in an amount of gained compound emulsifying agent, add prepared preparation of the present invention in an amount of previous embodiment respectively, stir while adding, solution is mixed becomes the preparation G1 to G4 of the present invention that settled solution promptly gets the following table composition.
Table 8: the composition of preparation of the present invention
| Preparation of the present invention | ????G1 | ????G2 | ????G3 | ????G4 |
| Eucalyptus oil (heavy %) tea oil (heavy %) valerian oil (heavy %) sandalwood oil (heavy %) Tween 80 (heavy %) sorbester p17 (heavy %) | ????21.?94％ ????21.94％ ????- ????0.55％ ????49.4％ ????6.18％ | ????41.6％ ????- ????- ????0.42％ ????51.2％ ????6.78％ | ????- ????47.2％ ????- ????0.34％ ????47.69％ ????4.72％ | ????36.8％ ????- ????9.2％ ????0.23％ ????46.1％ ????7.67％ |
The test of embodiment 9 antibacterial activities
Adopt the trace liquid diluting method, measure the antibacterial activity of preparation G1 of the present invention, G2, G3, G4, experimental result sees Table 9 and 10.
Table 9: minimum inhibitory concentration value (MIC)
| Sample number into spectrum | ?????????????????????????????????????MIC(V/V，％) | ||||
| Staphylococcus aureus | Escherichia coli | Shigella dysenteriae | Black-koji mould | Saccharomyces albicans | |
| Preparation G1 of the present invention | ????0.0125 | ????＞0.25 | ????0.125-0.25 | ??＞0.5 | ??＞0.5 |
| Preparation G2 of the present invention | ????0.0125 | ????0.125-0.25 | ????0.0625 | ??＞0.5 | ??＞0.5 |
| Preparation G3 of the present invention | ????0.025 | ????0.25 | ????0.25 | ??0.5 | ??0.5 |
| Preparation G4 of the present invention | ????0.0125-0.025 | ????＞0.25 | ????0.125 | ??＞0.5 | ??＞0.5 |
Table 10: minimum bactericidal concentration value (MBC)
| Sample number into spectrum | ?????????????????????????????????????MBC(V/V，％) | ||||
| Staphylococcus aureus | Escherichia coli | Shigella dysenteriae | Black-koji mould | Saccharomyces albicans | |
| Preparation G1 of the present invention | ??0.05 | ????＞0.25 | ????0.25 | ??＞0.5 | ＞0.5 |
| Preparation G2 of the present invention | ??0.05 | ????＞0.25 | ????0.125 | ??＞0.5 | ＞0.5 |
| Preparation G3 of the present invention | ??0.1 | ????＞0.25 | ????0.25 | ??＞0.5 | 0.5 |
| Preparation G4 of the present invention | ??0.01 | ????＞0.25 | ????0.25 | ??＞0.5 | ＞0.5 |
By table as seen, preparation of the present invention has significant bactericidal effect.
The test of embodiment 10 resisiting influenza virus
Adopt external direct deactivation method, Strain is the first type, influenza B virus international standard strain (first type: A3 Sydney/05/97, B-mode: B/ capital anti-/ 184), cell is a mdck cell, with the dilution preparation G1 of the present invention of difference, G2, G3, the viral liquid mixed in equal amounts of G4 and 100TCID50, in 37 ℃ and 30 minutes, getting 40 μ l respectively is inoculated in the cell hole of 96 orifice plates, establish virus control simultaneously, cell contrast and different dilution preparation contrasts of the present invention, day by day observe CPE, when virus control group CPE occurs ++ ++ the time termination test, carry out the blood clotting titer determination simultaneously.The results are shown in following table.
Table 11:MDCK cell toxicity test result
| The diluted sample degree | 1∶60 | ?1∶120 | ?1∶240 | ?1∶480 | ?1∶960 | ?1∶1920 | The cell contrast |
| Cell state | +++ | ?+ | ?- | ?- | ?- | ?- | - |
Table 12: influenza A virus neutralization test result
| The diluted sample degree | 1∶60 | ?1∶120 | ?1∶240 | ?1∶480 | 1∶960 | 1∶1920 | Virus control | The cell contrast |
| CPE | +++ | ?+ | ?- | ?- | ++ | +++ | ++++ | - |
| The blood clotting titre | - | ?- | ?- | ?- | ++ | ++ | ++++ | - |
Table 13: influenza B virus neutralization test result
| The diluted sample degree | 1∶60 | 1∶120 | ?1∶240 | ?1∶480 | 1∶960 | 1∶1920 | Virus control | The cell contrast |
| CPE | +++ | + | ?- | ?- | ++ | ++ | ++++ | - |
| The blood clotting titre | - | - | ?- | ?- | ++ | +++ | ++++ | - |
Annotate: "-" expression sample 100% inactivation of viruses
As seen from the above table, preparation of the present invention has activity to influenza A virus, and the present invention has activity to influenza B virus.Preparation of the present invention 1: 60,1: 120,1: 240,1: 480 dilution in 37 ℃ following 30 minutes can 100% deactivation first type and influenza B virus.1: 960 and 1: 1920 dilution of preparation of the present invention, but the first type and the influenza B virus of deactivation 25～50%.
Embodiment 11 kills the test of bacterium in the air
Adopt field trial, use preparation G1 of the present invention, G2, G3, G4 and supporting machine thereof, this machine is distributed to preparation of the present invention in the air, measure the bactericidal action of preparation of the present invention to air, before and after using preparation of the present invention, gather air sample, wherein use the preceding air sample of preparation of the present invention in contrast, the air sample after using is the sample of preparation bactericidal effect of the present invention; Sample was cultivated statistics total plate count, calculating sterilizing rate 48～72 hours in 37 ℃.
Table 14: to the bactericidal effect of air
| Service time | Sterilizing rate |
| 0 minute | ????- |
| 40 minutes | ????59.65％ |
| 60 minutes | ????67.16％ |
By table as seen, preparation of the present invention has the effect of obviously killing bacterium in the air
By above experiment as can be seen, use preparation of the present invention can significantly kill influenza first type virus, influenza B virus, Escherichia coli, staphylococcus aureus, shigella dysenteriae, black-koji mould, saccharomyces albicans etc.; And bacterium in the air had remarkable killing action.
Claims (10)
1, the preparation of a kind of germ capable of killing virus contains one or more natural plant essential oils of being selected from one group that eucalyptus oil, tea oil, valerian oil, sandalwood oil and other viral germ is made up of the natural plant essential oils of killing action or by the active ingredient of its extraction active component as germ capable of killing virus.
2, the preparation of a kind of germ capable of killing virus is by being selected from one or more natural plant essential oils in a group that eucalyptus oil, tea oil, valerian oil, sandalwood oil and other viral germ is made up of the natural plant essential oils of killing action or being made up of the active ingredient of its extraction.
3, the preparation of a kind of germ capable of killing virus, two or more essential oil or form in a group of forming by eucalyptus oil, tea oil, valerian oil and sandalwood oil by the active ingredient of its extraction.
4,, it is characterized in that when described preparation contains two or more described plants essential oil the content of described plants essential oil respectively is 0.05～15 part according to any preparation described in the claim 1 to 3.
5, the preparation of a kind of germ capable of killing virus contains 5～0.05 parts sandalwood oil, and 5～15 parts eucalyptus oil and/or 5～15 parts tea oil and/or 1～10 part valerian oil.
6, according to any preparation described in claim 1 to 3 and 5, it is characterized in that also containing auxiliary material and/or solvent, described auxiliary material is conventional or known auxiliary material and/or the emulsifier in this area, described solvent is the conventional or known solvent in this area.
7, the preparation of a kind of germ capable of killing virus, by (1) 5～0.05 part sandalwood oil, and 5～15 parts eucalyptus oil and/or 5～15 parts tea oil and/or 1～10 part valerian oil; (2) emulsifier is formed.
8, preparation according to claim 7 is characterized in that emulsifier is 0～100% (heavy %) Tween 80 and 100%～0% sorbester p17.
9, a kind of method for preparing the preparation of germ capable of killing virus comprises: (1) obtains plants essential oil, and mix (1) step gained plants essential oil (2) with auxiliary material.
10, claim 1 to 3,5,7 or 8 described preparations or according to the preparation that claim 9 makes are used for the purposes of air cleaning.
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| CN111297949A (en) * | 2020-03-19 | 2020-06-19 | 王鹏 | Essential oil mask and preparation method thereof |
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| CN113244433A (en) * | 2021-06-18 | 2021-08-13 | 宁波华木来生物科技有限公司 | Preparation method of bactericidal essential oil with fruit fragrance |
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