CN101455850A - Air disinfectant - Google Patents
Air disinfectant Download PDFInfo
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- CN101455850A CN101455850A CNA2007101621112A CN200710162111A CN101455850A CN 101455850 A CN101455850 A CN 101455850A CN A2007101621112 A CNA2007101621112 A CN A2007101621112A CN 200710162111 A CN200710162111 A CN 200710162111A CN 101455850 A CN101455850 A CN 101455850A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
Most air refreshing disinfectant sold in the prior market contains an artificially synthesized chemical sterilizer, and can be harmful to human body when the human body is often exposed in the air containing chemical products. Therefore, the invention provides an air refreshing disinfectant, and in particular relates to an air refreshing disinfectant which is not chemically synthesized and takes natural materials as raw materials and traditional Chinese medicine volatile oil as a main effective ingredient. The air refreshing disinfectant has the characteristics of bacteriostasis, antivirus, permanent aroma and economy and applicability, and is favorable for human health. In order to achieve the aims, the invention utilizes the characteristics of bacteriostasis, antivirus and permanent aroma of the traditional Chinese medicine volatile oil, and particularly adopts specified compositions of oleum eucalypti, clove oil, patchouli oil, forsythia oil and nepeta oil as active ingredients of the air refreshing disinfectant.
Description
Technical field
The present invention relates to a kind of air disinfectant, particularly a kind of is that the volatile oil of the middle Types of Medicine of raw material is the air disinfectant of main effective ingredient with non-chemically synthetic, natural material.
Technical background
Environment goes from bad to worse, and harmful substance increases in the air, and abnormal flavour, aerial infection influence people's emotion, health, work efficiency.Cold, influenza or upper respiratory tract infection are the commonly encountered diseases and the frequently-occurring diseases of winter-spring season, and it is propagated mainly by the spittle and air cross infection, and in the place that the crowd is dense, air is difficult for circulation, as hospital, school, company etc., incidence rate is very high especially.This mainly is owing to there is more pathogenic microorganism in the air in above-mentioned place, as influenza virus, rhinovirus, respiratory syncytial virus, adenovirus, Hemolytic streptococcus, staphylococcus, escherichia coli etc.Therefore, air being carried out disinfection is to prevent and control one of important measures of this cross infection.In order to kill airborne harmful microorganism and to remove abnormal flavour, air disinfectant is often used in Modern Family, hospital, hotel, restaurant and some other public arenas in air.But the air disinfectant of Xiao Shouing in the market contains the chemosterilant of synthetic mostly, often is exposed in this air that contains chemicals, may know from experience the people and cause certain injury.Therefore,, develop the pure and fresh sterile products of natural air safely and effectively, seem particularly important for purifying the air of a room.
Summary of the invention
The present invention is directed to the technical deficiency of existing with fresh air and disinfectant etc., disclose a kind of volatile oil with middle Types of Medicine is the air disinfectant of main effective ingredient, has antibacterial, antiviral, fragrant lasting, economic and practical characteristics, is of value to the health of human body.
For the tangible prescription of screening effect, selected staphylococcus aureus, Staphylococcus albus and three kinds of bacterial strains of escherichia coli and influenza A virus FM1 strain to carry out the evaluation of the antibacterial and antivirus action of this patent product.In common pathogenic bacteria and virus, staphylococcus, Bacterium entericum and influenza virus are antibacterial or the virus with important medical significance; These antibacterials or virus are distributed widely in nature, on air, soil, water and article, also are present in humans and animals skin and reach in the tract that communicates with the external world; Have 20%～50% people to carry pathogenic staphylococcus among the crowd, medical worker's carrying rate can be up to 70%; Staphylococcus aureus and escherichia coli are one of main 6 kinds of antibacterials that cause hospital infection, and wherein coli-infection rate is classified the first place as.Ministry of Health of China " disinfection technology standard " (version in 2002) is promptly clearly stipulated, in the test of disinfectant microbicidel, its microorganism comprises staphylococcus aureus, Staphylococcus albus and escherichia coli etc., and the strain of air sterilization effect qualification test regulation is a Staphylococcus albus.In addition, influenza A virus is the easiest to be popular in first, second, influenza virus C.Therefore, this patent is chosen the evaluation that three kinds of antibacterials such as staphylococcus aureus, Staphylococcus albus and escherichia coli with the meaning represented and influenza A virus FM1 virus are used for the antibacterial and antivirus action of following examples, and its result can reflect that patented product is used for air sterillization and truth and practical value when pure and fresh.
As mentioned above, the present invention is directed to the technical deficiency that has with fresh air and disinfectant etc. now, disclosing a kind of is the air disinfectant of main effective ingredient with traditional medicine volatile oil, has antibacterial, antiviral, lasting, the economic and practical characteristics of fragrance, is of value to the health of human body.
For reaching this purpose, the present invention proposes the active component of the particular combinations of a kind of Oleum Eucalypti, Oleum Caryophylli, patchouli oil, Fructus Forsythiae oil and Herba Schizonepetae oil as air disinfectant.
The optional substrate that contains of above-mentioned composition of the present invention.Containing under the situation of substrate, the ratio of substrate and volatile oil can change in the scope of broad, and impact effect not.
And do not have particular restriction for operable substrate, as long as it can cooperate reaching of the object of the invention.
Above-mentioned air disinfectant can be dispersed in the air by modes such as heating, sprayings, perhaps with this air disinfectant filtration, rinse air and reach disinfectant purpose with fresh air.
Compared with the prior art, the present invention has following advantage:
1, has antibacterial and antivirus action, fragrant odour, fresh air, elimination abnormal flavour, and can bring fresh and comfortable, all there sensation to user.Can be widely used in places such as family, office, commercial activity, hotel, compartment, particularly be applied to hospital, not only make the people clear-headed, comfortable, and can significantly reduce infectious chance.
2, product property is stable, and durable in use, Costco Wholesale is low, and consumer easily accepts.
3, production small investment, workable.This product processes is simple, ripe, and equipment investment is few, and is easy to operate.
4, prescription is made up of traditional medicine volatile oil, and product safety is reliable.
Specifically, technical scheme provided by the invention is as follows:
1. preparation, it contains compound volatile oil, and described volatile oil is Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
2, according to technical scheme 1 described preparation, it is a disinfectant preparation with fresh air.
3. according to technical scheme 1 or 2 described preparations, it is characterized in that described preparation also contains substrate.
4. according to technical scheme 1 or 2 described preparations, wherein said preparation is made up of Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
5, according to the described preparation of one of technical scheme 1-4, wherein Oleum Eucalypti is that myrtle Eucalyptus globulus Labill Eucalyptus globulus Labill., canella Camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections belong to the volatile oil that other plant obtains through extraction together.
6, according to the described preparation of one of technical scheme 1-5, wherein Fructus Forsythiae oil is the volatile oil that the dry fruit of Oleaceae plant Fructus Forsythiae Forsythia suspensa (Thunb.) Vahl obtains through extraction.
7, according to the described preparation of one of technical scheme 1-6, wherein Oleum Caryophylli is the volatile oil that the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb. obtains through extraction.
8, according to the described preparation of one of technical scheme 1-7, wherein patchouli oil is the dry stem of labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth. and the volatile oil that the Ye Jing extraction obtains.
9, according to the described preparation of one of technical scheme 1-8, wherein Herba Schizonepetae oil is the volatile oil that the herb of labiate Herba Schizonepetae Schizonepeta tenuifolia Briq. obtains through extraction.
10, according to the described preparation of one of technical scheme 1-9, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti is about 6～90%, and Fructus Forsythiae oil is about 3～70%, and Oleum Caryophylli is about 5～80%, and patchouli oil is about 1～70%, Herba Schizonepetae oil about 1～85%.
11, according to the described preparation of one of technical scheme 1-10, wherein the compound volatile oil each component is based on the cumulative volume of described compound volatile oil, by volume part proportioning is as follows: Oleum Eucalypti about 21～71%, Fructus Forsythiae oil about 8～48%, Oleum Caryophylli about 15～60%, patchouli oil is about 3～47%, Herba Schizonepetae oil about 3～53%.
12, according to the described preparation of one of technical scheme 1-11, wherein the compound volatile oil each component is based on the cumulative volume of described compound volatile oil, by volume part proportioning is as follows: Oleum Eucalypti about 18～63%, Fructus Forsythiae oil about 5～19%, Oleum Caryophylli about 6～27%, patchouli oil is about 6～16%, Herba Schizonepetae oil about 6～29%.
13, according to the described preparation of one of technical scheme 1-12, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti is about 43～52%, and Fructus Forsythiae oil is about 13～20%, and Oleum Caryophylli is about 21～30%, and patchouli oil is about 7～15%, Herba Schizonepetae oil about 7～15%.
14, according to the described preparation of one of technical scheme 1-13, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti is about 45%, and Fructus Forsythiae oil is about 14%, and Oleum Caryophylli is about 23%, and patchouli oil is about 9%, Herba Schizonepetae oil about 9%.
15, according to the described preparation of one of technical scheme 1-14, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is about 10:3:1:1:1.
16, according to the described preparation of one of technical scheme 1-15, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is about 15:4.5:7.5:3:7.
17, according to the described preparation of one of technical scheme 3-16, its mesostroma is the mixture of kerosene, isopropyl alcohol, dehydrated alcohol or its any combination.
18, according to technical scheme 17 described preparations, its mesostroma each component is based on the cumulative volume of described substrate, and by volume part proportioning is as follows:
Kerosene is about 50～90%,
Isopropyl alcohol or dehydrated alcohol about 10～50%.
19, according to technical scheme 17 described preparations, its mesostroma each component by volume part proportioning is as follows:
Kerosene is about 71.4%,
Isopropyl alcohol or dehydrated alcohol about 28.6%.
20., it is characterized in that described preparation is made up of compound volatile oil and substrate according to the preparation of one of technical scheme 3-19.
21, according to technical scheme 20 described preparations, wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil is about 1～100%, substrate about 0～99%.
22, according to technical scheme 20 described preparations, wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil is about 1～10%, substrate about 90～99%.
23, according to technical scheme 22 described preparations, wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil is about 2.5%, substrate about 97.5%.
24, according to technical scheme 22 described preparations, wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil is about 10%, substrate about 90%.
25, arbitrary described preparation is used to make the purposes of disinfecting air with fresh air in the technical scheme 1-24.
26. according to the purposes of technical scheme 25, it is dispersed in the air, thereby filtration, rinse air and reach disinfectant purpose with fresh air.
27. according to the purposes of technical scheme 25 or 26, described preparation is to be dispersed in the air by heating and/or spray pattern.
28. the preparation method of each described preparation in the technical scheme 1-24:
Described method comprises the step of extracting each described component in the described compound volatile oil.
29. according to technical scheme 28 described preparation methoies:
Described method comprises the step of each described component in the described compound volatile oil of following extraction:
Adopt steam distillation from the raw material of each component, to extract each component, and/or adopt supercritical fluid extraction from the raw material of each component, to extract each component.
30. according to technical scheme 28 or 29 described preparation methoies, described method also further comprises:
Measure each component of being extracted respectively;
With its mixing, optional stirring evenly, to obtain described compound volatile oil.
31. according to the preparation method of technical scheme 30, described method is further comprising the steps of:
Measure described substrate, the optional substrate that multicomponent is formed is mixed, optional again stirring evenly;
And the mixture that described substrate is added described each volatile oil to be to mix, and optional mixture with volatile oil and substrate stirs evenly, and described disinfectant preparation with fresh air.
Description of drawings
Fig. 1 is the sampled point plane graph of the embodiment of field experimentation.Reference numeral 1 is a sampled point.
The specific embodiment
In this article, the implication of " pure and fresh sterilization " is to instigate air to present the aromatic odor and the supression of nature and/or kill airborne common bacteria and/or virus.
" Oleum Eucalypti, Oleum Caryophylli, patchouli oil, Fructus Forsythiae oil and Herba Schizonepetae oil " herein formed, and is also referred to as compound volatile oil, herbal mixture volatilization wet goods.
In the following explanation that how to prepare each sample, the raw material of each component is in the compound volatile oil:
Oleum Eucalypti: general Oleum Eucalypti all can use, and a concrete example is to belong to the volatile oil that other plant obtains through extraction together from myrtle Eucalyptus globulus Labill Eucalyptus globulus Labill., canella Camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections.(commercial goods of using among the embodiment described herein is available from Jishui County, Jiangxi Province Jiangxi Xu Xiangliaoyouchang, and lot number is 20060825.)
Fructus Forsythiae oil, general Fructus Forsythiae oil all can use, and concrete example is the volatile oil that the dry fruit from Oleaceae plant Fructus Forsythiae Forsythia suspensa (Thunb.) Vahl. obtains through extraction.(commercial goods of using among the embodiment described herein is available from Jishui County, Jiangxi Province Jiangxi Xu Xiangliaoyouchang, and lot number is 20060825.)
Oleum Caryophylli, general Oleum Caryophylli all can use, and concrete example is the volatile oil that the dry flower from plant clove of myrtaceae Eugenia caryophyllata Thunb. obtains through extraction.(commercial goods of using among the embodiment described herein is available from Jishui County, Jiangxi Province Jiangxi Xu Xiangliaoyouchang, and lot number is 20060825.)
Patchouli oil, general patchouli oil all can use, and concrete example is that dry stem and the Ye Jing from labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth. extracts the volatile oil that obtains.(commercial goods of using among the embodiment described herein is available from Guangzhou pot-pourri company limited, and lot number is 20060901.)
Herba Schizonepetae oil, general Herba Schizonepetae oil all can use, and concrete example is the volatile oil that the herb from labiate Herba Schizonepetae Schizonepeta tenuifolia Briq. obtains through extraction.(commercial goods of using among the embodiment described herein is available from Jishui County, Jiangxi Province Jiangxi Xu Xiangliaoyouchang, and lot number is 20060825.)
Except that buying the commercially available prod, also can adopt steam distillation or supercritical fluid extraction to extract above-mentioned volatile oil according to a conventional method from Chinese crude drug, perhaps also can adopt other existing known extracting method to extract.
Above-mentioned Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil also can extract from the plant of other section, genus respectively.
Wherein Oleum Eucalypti, patchouli oil are respectively by " carrying out quality monitoring under 2005 editions first the 280th, 272 page of items of Chinese pharmacopoeia, Oleum Caryophylli is by " carrying out quality monitoring under 1953 editions first the 7th page of items of Chinese pharmacopoeia, set up the quality standard of Fructus Forsythiae oil, Herba Schizonepetae oil voluntarily and monitor.
Be non-limiting example of the present invention below, below prescription (unless specifically stated otherwise) all is the parts by volume proportioning.
Embodiment 1
The bacteriostasis of single and herbal mixture volatile oil (antibacterial around-France)
Sample:
Sample 1: Oleum Eucalypti
Sample 2: Fructus Forsythiae oil
Sample 3: patchouli oil
Sample 4: Oleum Caryophylli
Sample 5: Herba Schizonepetae oil
Sample 6: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 10:3:1:1:1 ratio.
Preparation method: measure Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml respectively, mix, stir evenly, promptly.
Experimental strain:
Staphylococcus aureus (26112), escherichia coli (44113), Staphylococcus albus (26069), Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides by Beijing.
Test method:
16～18 hours liquid cultures with above-mentioned three kinds of bacterium, evenly coat the ordinary flat surface respectively with aseptic cotton carrier, dried slightly, inhale the drug sensitive test paper that quantitative sample medicinal liquid is arranged with the aseptic nipper gripping, be tiled in the planar surface of above-mentioned inoculated bacteria, a flat board is put 3 drug sensitive test papers, puts 37 ℃ of cultivations, next day observed result.Result of the test sees Table 1.
Table 1 single medicinal material volatile oil fungistatic effect result of the test (inhibition zone diameter, mm, n=2～5, X+SD)
Annotate:
Comprehensive antibacterial index, D=A * 0.3+B * 0.2+C * 0.5, down together.
N represents to test number of repetition (down together).
The result shows that Oleum Eucalypti, Fructus Forsythiae oil, patchouli oil, Oleum Caryophylli, Herba Schizonepetae oil all have inhibitory action in various degree to staphylococcus aureus, Staphylococcus albus, colibacillary growth.Wherein Oleum Caryophylli is the strongest to the effect of staphylococcus aureus, and Herba Schizonepetae oil is the strongest to colibacillary effect.
Owing to used three kinds of antibacterials, and sample is different to the action intensity of different bacterium, be difficult to by the whole fungistatic effect of simple more different samples to the inhibition effect assessment sample of three kinds of antibacterials, therefore need carry out overall merit, this patent adopts composite index law to estimate.
Composite index law is a kind of comprehensive analysis method commonly used, and by giving sample the weight different to the fungistatic effect of three kinds of antibacterials, the simple addition of fungistatic effect with after the consideration weight promptly draws a comprehensive antibacterial index (computing formula sees Table 1 footnote).The size of weight is to determine according to the importance of each factor and/or to contribution as a result, herein because coccus is represented the different antibacterial of two classes respectively with bacillus, so the weight of coccus and bacillus all gives 0.5; Because coccus has been used two kinds, wherein the staphylococcus aureus pathogenicity is stronger, is respectively 0.3 and 0.2 so give two kinds of coccus weights.The result shows that the comprehensive antibacterial index of sample 6 (herbal mixture volatile oil) is the highest, shows that the fungistatic effect of herbal mixture volatile oil is better than single volatile oil, and the reasonability and the superiority of prompting compound recipe have reflected its novelty and creativeness.
Have or not collaborative potentiation in order further to understand after single volatile oil is formed compound recipe, according to single volatile oil separately fungistatic effect and sample 6 in each single medicinal material volatile oil proportion, the comprehensive antibacterial index of the theory of computation, the result is 10.1.This is starkly lower than the comprehensive antibacterial index 15.0 of sample 6 reality, prompting single volatile oil compound recipe is formed the synergism that has produced each other behind the sample 6, fungistatic effect is strengthened, this has confirmed the preceding paragraph by the conclusion that the comprehensive antibacterial exponential direct comparison of the actual measurement of compound recipe and single volatile oil draws, and the novelty and the creativeness of compound recipe has been described.
Embodiment 2
The bacteriostasis (antibacterial around-France) of different proportioning herbal mixture volatile oil
Sample:
Sample 7: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 5:1.5:2.5:2:3 ratio.
Preparation method: measure Oleum Eucalypti 357.1ml, Fructus Forsythiae oil 107.1ml, Oleum Caryophylli 178.6ml, patchouli oil 142.9ml, Herba Schizonepetae oil 214.3ml respectively, mix, stir evenly, promptly.
Sample 8: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 5:3:5:3:5 ratio.
Preparation method: measure Oleum Eucalypti 238.1ml, Fructus Forsythiae oil 142.9ml, Oleum Caryophylli 238.1ml, patchouli oil 142.9ml, Herba Schizonepetae oil 238.1ml respectively, mix, stir evenly, promptly.
Sample 9: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 5:4.5:7.5:4:7 ratio.
Preparation method: measure Oleum Eucalypti 178.6ml, Fructus Forsythiae oil 160.7ml, Oleum Caryophylli 267.9ml, patchouli oil 142.9ml, Herba Schizonepetae oil 250.0ml respectively, mix, stir evenly, promptly.
Sample 10: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 10:1.5:2.5:3:7 ratio.
Preparation method: measure Oleum Eucalypti 416.7ml, Fructus Forsythiae oil 62.5ml, Oleum Caryophylli 104.2ml, patchouli oil 125.0ml, Herba Schizonepetae oil 291.7ml respectively, mix, stir evenly, promptly.
Sample 11: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 10:3:5:4:3 ratio.
Preparation method: measure Oleum Eucalypti 400.0ml, Fructus Forsythiae oil 120.0ml, Oleum Caryophylli 200.0ml, patchouli oil 160.0ml, Herba Schizonepetae oil 120.0ml respectively, mix, stir evenly, promptly.
Sample 12: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 10:4.5:7.5:2:5 ratio.
Preparation method: measure Oleum Eucalypti 344.8ml, Fructus Forsythiae oil 155.2ml, Oleum Caryophylli 258.6ml, patchouli oil 69.0ml, Herba Schizonepetae oil 172.4ml respectively, mix, stir evenly, promptly.
Sample 13: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 15:1.5:2.5:4:5 ratio.
Preparation method: measure Oleum Eucalypti 535.7ml, Fructus Forsythiae oil 53.6ml, Oleum Caryophylli 89.3ml, patchouli oil 142.9ml, Herba Schizonepetae oil 178.6ml respectively, mix, stir evenly, promptly.
Sample 14: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 15:3:5:2:7 ratio.
Preparation method: measure Oleum Eucalypti 468.8ml, Fructus Forsythiae oil 93.8ml, Oleum Caryophylli 156.3ml, patchouli oil 62.5ml, Herba Schizonepetae oil 218.8ml respectively, mix, stir evenly, promptly.
Sample 15: the mixture that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed in the 15:4.5:7.5:3:3 ratio.
Preparation method: measure Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml respectively, mix, stir evenly, promptly.
Measure the bacteriostasis of above-mentioned sample by the method for embodiment 1, result of the test sees Table 2.
The different proportioning Chinese medicine compound of table .2 volatile oil bacteriostatic test result (inhibition zone diameter, mm, n=3, X+SD)
(ratio that contained in embodiment sample 7～15 of each volatile oil is Oleum Eucalypti 17.9～53.6%, Fructus Forsythiae oil 5.4～16.1%, Oleum Caryophylli 8.9～26.8%, patchouli oil 6.3～16.0%, Herba Schizonepetae oil 9.1～29.2%.)
The result shows that the product of above-mentioned 9 prescriptions all has fungistatic effect.Calculate the antibacterial index of synthesis in theory of each prescription, be respectively: 11.4,11.7,11.8,11.5,10.9,11.6,10.9,11.5,11.1, all be lower than the comprehensive antibacterial index of actual measurement (D row in the table 2) of each correspondence, illustrate that 9 prescriptions have all produced synergism by compatibility aspect antibacterial, so the prescription of these 9 samples has novelty and creativeness too.
In 9 prescriptions, sample 7,11,12,13,14 has fungistatic effect, and sample 8,9,10 has fungistatic effect preferably, and sample 15 fungistatic effects are best.The ratio of each volatile oil is in the sample 15: Oleum Eucalypti 45.5%, Fructus Forsythiae oil 13.6%, Oleum Caryophylli 22.7%, patchouli oil 9.1%, Herba Schizonepetae oil 9.1%.
Above-mentioned test is by volatile oil different proportion orthogonal design L
9(3
4) and the result that obtains further analyzes as can be known its result according to orthogonal design: the ratio of Oleum Eucalypti in prescription changes, and be little to the fungistatic effect influence; The ratio of Fructus Forsythiae oil, Oleum Caryophylli and Herba Schizonepetae oil changes has considerable influence to fungistatic effect, fungistatic effect is along with their ratio increases and increases, the ratio of patchouli oil has the greatest impact to fungistatic effect, and during the ratio 6.3～16.0% changed, fungistatic effect was to increase afterwards earlier to reduce.Drawing the best ratio of theoretic fungistatic effect according to the information of the foregoing description is 15:4.5:7.5:3:7, and promptly Oleum Eucalypti 40.5%, Fructus Forsythiae oil 12.2%, Oleum Caryophylli 20.3%, patchouli oil 8.1%, Herba Schizonepetae oil 18.9%.
Optimal proportion and above-mentioned " ratio of Oleum Eucalypti in prescription changes, and be little to the fungistatic effect influence thus; The ratio of Fructus Forsythiae oil, Oleum Caryophylli and Herba Schizonepetae oil changes has considerable influence to fungistatic effect, and fungistatic effect increases along with their ratio and increases " discovery, can reason out, each volatile oil components contents scope can be at following range changing:
Oleum Eucalypti 43～52%, Fructus Forsythiae oil 13～20%, Oleum Caryophylli 21～30%, patchouli oil 7～15%, Herba Schizonepetae oil 7～15%; Or
Oleum Eucalypti 18～63%, Fructus Forsythiae oil 5～19%, Oleum Caryophylli 6～27%, patchouli oil 6～16%, Herba Schizonepetae oil 6～29%, or Oleum Eucalypti 21～71%, Fructus Forsythiae oil 8～48%, Oleum Caryophylli 15～60%, patchouli oil 3～47%, Herba Schizonepetae oil 3～53%;
Perhaps
Oleum Eucalypti 6～90%, Fructus Forsythiae oil 3～70%, Oleum Caryophylli 5～80%, patchouli oil 1～70%, Herba Schizonepetae oil 1～85%.
Explanation for above-mentioned scope:
Wherein because the ratio of Oleum Eucalypti in prescription changes, little to the fungistatic effect influence, the variation of the content upper and lower limit of Oleum Eucalypti can be very big; And the ratio of Fructus Forsythiae oil, Oleum Caryophylli and Herba Schizonepetae oil changes, and makes the legitimate inference near the content of foundation embodiment, considers that its fungistatic effect increases along with their ratio and the fact that increases, thus its upper limit actual can be than among the embodiment bigger.
In addition, because the variation of feasible content in a scope of each component is successional in the prescription, therefore any combination between the end points of the broad scope of disclosed point of embodiment and above-mentioned plaintext record also is feasible, also promptly belongs to the clear and definite scope of disclosure of the present invention.Equally, any coupling between any content range of a component and any content range of other any component also is feasible, belongs to the clear and definite disclosed part of the present invention.
Embodiment 3
The bacteriostasis (antibacterial around-France) of different proportioning herbal mixture volatile oil
Sample:
Sample 6: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (10:3:1:1:1)
Preparation method: measure Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml respectively, mix, stir evenly, promptly.
Sample 15: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15:4.5:7.5:3:3)
Preparation method: measure Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml respectively, mix, stir evenly, promptly.
Sample 16: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15:4.5:7.5:3:7)
Preparation method: measure Oleum Eucalypti 405.4ml, Fructus Forsythiae oil 121.6ml, Oleum Caryophylli 202.7ml, patchouli oil 81.1ml, Herba Schizonepetae oil 189.2ml respectively, mix, stir evenly, promptly.
Measure the bacteriostasis of above-mentioned sample by the method for embodiment 1, result of the test sees Table 3.
The different proportioning sample of table 3 traditional medicine volatile oil inhibition zone result of the test (inhibition zone diameter, mm, n=3, X ± SD)
The result shows that the product of above-mentioned 3 prescriptions all has fungistatic effect.The optimization of C of sample 16 for calculating according to the result in the example 2, the result shows that its fungistatic effect is better.Calculate the antibacterial index 11.5 of its synthesis in theory, be lower than it and survey comprehensive antibacterial index 14.3, proved that further compatibility of drugs produces the enhanced synergism of curative effect; Sample 16 prescriptions have novelty and creativeness.
Embodiment 4
The bacteriostasis (tube dilution method) of different proportioning herbal mixture volatile oil
Sample:
Sample 6: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (10:3:1:1:1)
Preparation method: measure Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml respectively, mix, stir evenly, promptly.
Sample 15: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15:4.5:7.5:3:3)
Preparation method: measure Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml respectively, mix, stir evenly, promptly.
Sample 16: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15:4.5:7.5:3:7)
Preparation method: measure Oleum Eucalypti 405.4ml, Fructus Forsythiae oil 121.6ml, Oleum Caryophylli 202.7ml, patchouli oil 81.1ml, Herba Schizonepetae oil 189.2ml respectively, mix, stir evenly, promptly.
Experimental technique:
Sample 6,15,16 each 3ml are added the 1ml Tween 80 respectively make its dissolving, adding nutrient broth again is mixed with every ml and contains the crude drug amount and be respectively 0.30ml, 0.15ml, 0.075ml, 0.0375ml, 0.01875ml, 0.009375ml, 0.004688ml, 0.002344ml, 0.001172ml, the dilute sample of totally 9 drug level, every pipe total amount 1ml, heat sterilization.
Control tube: the strain contrast, for the culture medium that does not contain medicine adds test organisms; The medicine contrast is not for adding the medicinal liquid of test organisms.
It is 10 that each concentration pipe of every row's medicinal liquid and strain control tube add concentration respectively
6Cfuml
-1Test organisms liquid (8 hours cultures) 0.1ml, cultivated 24 hours for 37 ℃, again each concentration is got a ring streak inoculation on the solid nutrient agar panel with inoculating loop, cultivate 24 hours observed results for 37 ℃.With the turbidity is that each pipe of index naked-eye observation has asepsis growth; With the naked eye observe each flat board have or not colony growth.
Judge minimal inhibitory concentration (MIC), refer to the minimum drug level that complete inhibition test growth is contained.
Judge minimal bactericidal concentration (MBC), refer to kill fully the contained minimum drug level of test strain growth.
Result of the test sees Table 4.
The different proportioning herbal mixture of table 4 volatile oil is to MIC and MBC measurement result (the μ lml of 3 kinds of antibacterials
-1)
MIC and MBC are antibacterial and germ-resistant two important indicators of medicine, and its antibacterial effect that is worth the bright medicine of novel more is good more, utilizes this two parameters, and concentration and its fungistatic effect of sample 6,15,16 are estimated.The result shows, above-mentioned 3 samples all have in various degree the bactericidal action that presses down to test strain, and the MIC of each strain is between 2.34 μ lml
-1～75.0 μ lml
-1Between, the MBC of each strain is between 4.69 μ lml
-1～750 μ lml
-1Between, the comprehensive bactericidal index minimum of the MBC of sample 16 wherein, and its MIC and sample 15 is approaching, illustrates that its comprehensive bactericidal effect is best, comprehensive fungistatic effect is also better.
Embodiment 5
The antivirus action of different proportioning herbal mixture volatile oil
Sample:
Sample 6: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (10:3:1:1:1)
Preparation method: measure Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml respectively, mix, stir evenly, promptly.
Sample 15: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15:4.5:7.5:3:3)
Preparation method: measure Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml respectively, mix, stir evenly, promptly.
Sample 16: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15:4.5:7.5:3:7)
Preparation method: measure Oleum Eucalypti 405.4ml, Fructus Forsythiae oil 121.6ml, Oleum Caryophylli 202.7ml, patchouli oil 81.1ml, Herba Schizonepetae oil 189.2ml respectively, mix, stir evenly, promptly.
The positive control drug virazole, lot number 0301006, the medical professionals is provided by Hubei.
Seed culture of viruses:
Influenza A virus FM1 strain is provided by Inst. of Medicinal Biological Technology, Chinese Academy of Medical Sciences, go down to posterity in MDCK Madin-Darby canine kidney(cell line) (MDCK) (Madin-Darby Canine Kidney cell, Madin-Darby canine kidney cell), judge its titre with pathological changes and chicken erythrocyte blood clotting method, at 37 ℃, 5%CO
2Cultivate in the incubator.
Experimental technique:
Require to carry out by " study of tcm new drug guide " (bureau of drug policy ﹠ administration of Ministry of Health of the People's Republic of China, version in 1994), more than the virus test on 96 hole micro plates, carry out every hole 0.1ml.Cell number is 1 * 10
5After individual/ml grew up to monolayer, the medicinal liquid of keeping was diluted to 4 variable concentrations in inciting somebody to action, behind the jettisoning original fluid variable concentrations Chinese medicine sample is added in the cell hole, and every hole 0.1ml, the minimum dilution factor that does not occur pathological changes with cell is the nontoxic boundary of this medicinal liquid pair cell.On mdck cell, measure the TCID of challenge virus simultaneously
50(50% tissue culture infective dose, tissue culture infective dose).Establish virus control, cell contrast, drug toxicity contrast and positive drug contrast (virazole) during test.Put 37 ℃, 5%CO
2Cultivate in the incubator.Under inverted microscope, observe pathological changes and record every day.When the virus control porocyte occurs ++ during+pathological changes, continue to observe then stopping in 7 days.The degree that pathological changes appears in cell is by 6 grade standard records :-: the cell growth is normal, no pathological changes appearance; " ± ": cytopathy is less than 10% of whole cell monolayer; "+": cytopathy is less than 25% of whole cell monolayer; " ++ ": cytopathy is less than 50% of whole cell monolayer; " +++": cytopathy is less than 75% of whole cell monolayer; " ++ ++ ": cytopathy accounts for more than 75% of whole cell monolayer.Result of the test sees Table 5.
The different proportioning samples of table 5 traditional medicine volatile oil are to influenza A virus FM1 inhibitory action
Annotate: "+" suppresses "-" to viral unrestraint effect fully to virus
The result shows, at infective virus TCID
50Under 100 dosage, virazole 100 μ gml
-1FM1 is had obvious inhibitory action, and No. 15, sample is at 160 μ lml under inferior toxicity concentration
-1FM1 there is certain inhibitory action.Interpret sample 15 also has the effect that suppresses airborne common virus, has the effect of air sterillization.(in embodiment subsequently, selecting the prescription of sample 15 to carry out the object that one of them continues research)
Embodiment 6
The simulated field test
Sample:
Chinese medicine air disinfectant 1: it is formulated to press 1:9 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil is that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed by 15:4.5:7.5:3:3, and substrate is that kerosene and dehydrated alcohol are formed by 2.5:1.
Preparation method: measure Oleum Eucalypti 45.5ml, Fructus Forsythiae oil 13.6ml, Oleum Caryophylli 22.7ml, patchouli oil 9.1ml, Herba Schizonepetae oil 9.1ml respectively, mix, stir evenly, get herbal mixture volatile oil, standby; Measure kerosene 642.9ml and dehydrated alcohol 257.1ml respectively, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, promptly.
Equipment:
Test strain: Staphylococcus albus 8032 is provided by Military Medical Science Institute.Strain algebraically was the 6th～8 generation, prepared bacteria suspension with ordinary broth.
Aerosol generator, 90% above particle Φ≤5 μ m.
Aerosol chamber's volume is 10m
3
Sampler: FA-1 type impacting type air microorganism sampler.
Method:
Test basis: Ministry of Public Health " disinfection technology standard " (version in 2002) 2.1.3.
23～24 ℃ of test temperatures, relative humidity 50～58%.
Microbiological contamination aerosol apparatus flow is 21.7L/min, and spray time 5min stirs 5min, leaves standstill 5min.
Sterilization method: during test with 2ml/m
3Amount, laboratory is sprayed disinfective action 60min post-sampling with the electronic aerosol atomizer of QPQ-2300 type.Matched group replaces disinfectant with deionized water, and other operate same test group.Test repeats 3 times.
Adopt FA-1 type impacting type air microorganism sampler, sampling flow is 28.3L/min, establishes a point sampling in chamber central authorities apart from ground 1.0m.Sample when the disinfective action time is 0min, 60min, the matched group sampler sampling time is respectively 5s, 5s, and the test group sampler sampling time is respectively 5s, 1min, and the sampling back is measured different time and deposited viable count, is calculated as follows killing rate:
N
t: the natural extinction rate of antibacterial in the air;
V
0With V
t: be respectively matched group air bacteria containing amount of different time before on-test and in the process of the test;
K
t: disinfect killing rate to antibacterial in the air;
V
0' with V
t': be respectively the air bacteria containing amount that test group is disinfected different time in preceding and the disinfecting process.
The results are shown in Table 6.
1 pair of staphylococcic killing effect of white of table 6 Chinese medicine air disinfectant
Negative control: diluent, the equal asepsis growth of culture medium.
The result shows, Chinese medicine air disinfectant 1 disinfective action 60min, and to the staphylococcic killing rate of white, 3 test killing rates are all more than 96.30%.Illustrate that Chinese medicine air disinfectant 1 can kill airborne common bacteria, have the effect of air disinfection.
Embodiment 7
Field trial
Sample:
Chinese medicine air disinfectant 2: it is formulated to press 1:39 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil is that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed by 15:4.5:7.5:3:3, and substrate is that kerosene and isopropyl alcohol are formed by 2.5:1.
Preparation method: measure Oleum Eucalypti 11.4ml, Fructus Forsythiae oil 3.4ml, Oleum Caryophylli 5.7ml, patchouli oil 2.3ml, Herba Schizonepetae oil 2.3ml respectively, mix, stir evenly, get herbal mixture volatile oil, standby; Measure kerosene 696.4ml and isopropyl alcohol 278.6ml respectively, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, promptly.
Chinese medicine air disinfectant 3: it is formulated to press 1:9 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil is that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed by 15:4.5:7.5:3:3, and substrate is that kerosene and isopropyl alcohol are formed by 2.5:1.
Preparation method: measure Oleum Eucalypti 45.5ml, Fructus Forsythiae oil 13.6ml, Oleum Caryophylli 22.7ml, patchouli oil 9.1ml, Herba Schizonepetae oil 9.1ml respectively, mix, stir evenly, get herbal mixture volatile oil, standby; Measure kerosene 642.9ml and isopropyl alcohol 257.1ml respectively, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, promptly.
Experimental enviroment and instrument:
Room volume: 70m
3
Temperature and humidity: room temperature: 31 ℃, room relative humidity: 85%
Spraying instrument: electronic aerosol atomizer, model: DQP-600, producer: Beijing Song Yuan Huaxing development in science and technology company limited
Sterilization and sampling:
Sampled point is as shown below, carries out 2 samplings altogether, when the sampling time, point was respectively 0h, during 2h.
The method of sampling: on 5 sampled points as shown in Figure 1, each point is put two diameter 9cm flat boards, and height of sampling is 1.0m, opens culture dish, exposes after 10 minutes, and lid is built, and sample calculates clump count and also is calculated as follows extinction rate in 37 ℃ of cultivations 24 hours.
Sterilizing operation: the dosage by every cubic metre of 1ml carries out, and the electricity consumption colloidal sol aerosol apparatus of taking offence evenly is sprayed on the room, been sprayed, sterilization 2h.
The statistical test method:
The results are shown in Table 7,8.
Disinfection Effect under the table 7 Chinese medicine air disinfectant 2 on-the-spot air-tight states
Disinfection Effect under the table 8 Chinese medicine air disinfectant 3 on-the-spot air-tight states
The result shows, under the air-tight state, carries out pure and fresh sterilization with air in 2,3 pairs of spaces of Chinese medicine air disinfectant at the scene, can make after the sterilization that bacterial number reduces about 71% and 76% in the air, and minimizing has the significance meaning.Illustrate that Chinese medicine air disinfectant 2,3 can kill airborne antibacterial, have the disappear effect of bacterium of air.
According to the data 1:9 and the 1:39 of the foregoing description, and effect as seen, and the ratio of substrate and volatile oil can change in the scope of broad, and what influence is effect do not had.All can implement the present invention in following scope:
Compound volatile oil〉0～≤ 100%, substrate 0～99%; "〉0 wherein " expression is greater than 0.
Compound volatile oil 1～100%, substrate 0～99%;
Or compound volatile oil 1～10%, substrate 90～99%.
And, do not have particular restriction for operable choice of base, as long as it can cooperate reaching of the object of the invention, this is that those skilled in the art can simply measure selection.The example of substrate includes but not limited to as kerosene, isopropyl alcohol, dehydrated alcohol or the like, or the mixture of its any combination.
Embodiment 8
Acute oral toxicity test
Test objective:
Observe the Chinese medicine air disinfectant and once give the toxic reaction that produced behind the animal, estimate the safety to human body when practical application of this pure and fresh disinfectant.
Sample:
Chinese medicine air disinfectant 2: it is formulated to press 1:39 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil is that Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil are formed by 15:4.5:7.5:3:3, and substrate is that kerosene and isopropyl alcohol are formed by 2.5:1.
Preparation method: measure Oleum Eucalypti 11.4ml, Fructus Forsythiae oil 3.4ml, Oleum Caryophylli 5.7ml, patchouli oil 2.3ml, Herba Schizonepetae oil 2.3ml respectively, mix, stir evenly, get herbal mixture volatile oil, standby; Measure kerosene 696.4ml and isopropyl alcohol 278.6ml respectively, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, promptly.
What use during test is
Oleum Arachidis hypogaeae semen (the date of manufacture: 2007-01-30) (also can using certainly of other) dilution.
Experimental animal:
NIH (National Institute of Health, NIH) be white mice (quality certification number: 0027327), male and female half and half, body weight 18～22g is provided by Guangdong Province's Experimental Animal Center.
Test method:
Getting NIH is 20 of mices, male and female half and half, body weight 18～22g.The animal adaptability is raised 2d.Animal overnight fasting before the administration, freely drink water.Get an amount of Oleum Arachidis hypogaeae semen dilution 5.0287g Chinese medicine air disinfectant 2 during test to 20ml, by the 20ml/kg body weight is that dosage 5000mg/kg body weight is once irritated stomach, at once observe reaction of animals, observed for two weeks continuously, record animal toxicity response situation and dead the distribution, simultaneously dead animal is performed an autopsy on sb, record pathological changes situation is judged LD
50Whether greater than the 5000mg/kg body weight.
Result and conclusion:
Result of the test shows: Chinese medicine air disinfectant 2 is the disposable filling stomach of 5000mg/kg body weight according to dosage, and the overt toxicity reaction does not appear in animal.The reaction of animal mainly shows as movable the minimizing after the administration, and minority excitement occurs, beats; 2 hours animal activities recover normal substantially after the administration; No animal occurs dead.So judge its LD
50Greater than the 5000mg/kg body weight.
According to the toxicity assessment standard in Ministry of Health of the People's Republic of China's " disinfection technology standard " (version in 2002):
LD
50Nontoxic greater than the true border of 5000mg/kg body weight person;
LD
50For 501mg/kg～5000mg/kg body weight person belongs to low toxicity;
LD
50For 51mg/kg～500mg/kg body weight person belongs to medium poison;
LD
50For 1mg/kg～50mg/kg body weight person belongs to high poison;
LD
50Belong to severe toxicity less than 1mg/kg body weight person.
Because the LD of this Chinese medicine air disinfectant 2
50Greater than the 5000mg/kg body weight, can think to belong to nontoxic product.
Conclusion
That the Chinese medicine air disinfectant has is antibacterial significantly, sterilization and even antiviral activity, it writes out a prescription novel, can obviously suppress the growth of air middle school common pathogen, animal dead and tangible toxic reaction do not appear in the oral 5000mg/kg of animal, point out it safe and effective, its abnormal smells from the patient is pure and fresh, can be used for air sterillization and pure and fresh.
In addition, embodiment herein is all non-limiting example, and related numerical value unless specialize, also can be about value.Any combination between the end points of disclosed point of embodiment and generalized scope also is a scope disclosed by the invention; Any coupling between any content range of a component and any content range of other component also is a part disclosed by the invention.Also belong to a part of the present invention according to variation of the present invention.
Claims (31)
1. preparation, it contains compound volatile oil, and described volatile oil is Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
2, preparation according to claim 1, it is a disinfectant preparation with fresh air.
3. preparation according to claim 1 and 2 is characterized in that described preparation also contains substrate.
4. preparation according to claim 1 and 2, wherein said preparation is made up of Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
5, according to the described preparation of one of claim 1-4, wherein Oleum Eucalypti is that myrtle Eucalyptus globulus Labill Eucalyptus globulus Labill., canella Camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections belong to the volatile oil that other plant obtains through extraction together.
6, according to the described preparation of one of claim 1-5, wherein Fructus Forsythiae oil is the volatile oil that the dry fruit of Oleaceae plant Fructus Forsythiae Forsythia suspensa (Thunb.) Vahl obtains through extraction.
7, according to the described preparation of one of claim 1-6, wherein Oleum Caryophylli is the volatile oil that the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb. obtains through extraction.
8, according to the described preparation of one of claim 1-7, wherein patchouli oil is the dry stem of labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth. and the volatile oil that the Ye Jing extraction obtains.
9, according to the described preparation of one of claim 1-8, wherein Herba Schizonepetae oil is the volatile oil that the herb of labiate Herba Schizonepetae Schizonepeta tenuifolia Briq. obtains through extraction.
10, according to the described preparation of one of claim 1-9, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti is about 6～90%, and Fructus Forsythiae oil is about 3～70%, and Oleum Caryophylli is about 5～80%, and patchouli oil is about 1～70%, Herba Schizonepetae oil about 1～85%.
11, according to the described preparation of one of claim 1-10, wherein the compound volatile oil each component is based on the cumulative volume of described compound volatile oil, by volume part proportioning is as follows: Oleum Eucalypti about 21～71%, Fructus Forsythiae oil about 8～48%, Oleum Caryophylli about 15～60%, patchouli oil is about 3～47%, Herba Schizonepetae oil about 3～53%.
12, according to the described preparation of one of claim 1-11, wherein the compound volatile oil each component is based on the cumulative volume of described compound volatile oil, by volume part proportioning is as follows: Oleum Eucalypti about 18～63%, Fructus Forsythiae oil about 5～19%, Oleum Caryophylli about 6～27%, patchouli oil is about 6～16%, Herba Schizonepetae oil about 6～29%.
13, according to the described preparation of one of claim 1-12, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti is about 43～52%, and Fructus Forsythiae oil is about 13～20%, and Oleum Caryophylli is about 21～30%, and patchouli oil is about 7～15%, Herba Schizonepetae oil about 7～15%.
14, according to the described preparation of one of claim 1-13, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti is about 45%, and Fructus Forsythiae oil is about 14%, and Oleum Caryophylli is about 23%, and patchouli oil is about 9%, Herba Schizonepetae oil about 9%.
15, according to the described preparation of one of claim 1-14, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is about 10:3:1:1:1.
16, according to the described preparation of one of claim 1-15, wherein by volume part proportioning is as follows for the compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is about 15:4.5:7.5:3:7.
17, according to the described preparation of one of claim 3-16, its mesostroma is the mixture of kerosene, isopropyl alcohol, dehydrated alcohol or its any combination.
18, according to the described preparation of claim 17, its mesostroma each component is based on the cumulative volume of described substrate, and by volume part proportioning is as follows:
Kerosene is about 50～90%,
Isopropyl alcohol or dehydrated alcohol about 10～50%.
19, according to the described preparation of claim 17, its mesostroma each component by volume part proportioning is as follows:
Kerosene is about 71.4%,
Isopropyl alcohol or dehydrated alcohol about 28.6%.
20., it is characterized in that described preparation is made up of compound volatile oil and substrate according to the preparation of one of claim 3-19.
21, according to the described preparation of claim 20, wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil is about 1～100%, substrate about 0～99%.
22, according to the described preparation of claim 20, wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil is about 1～10%, substrate about 90～99%.
23, according to the described preparation of claim 22, wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil is about 2.5%, substrate about 97.5%.
24, according to the described preparation of claim 22, wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil is about 10%, substrate about 90%.
25, arbitrary described preparation is used to make the purposes of disinfecting air with fresh air in the claim 1-24.
26. according to the purposes of claim 25, it is dispersed in the air, thereby filtration, rinse air and reach disinfectant purpose with fresh air.
27. according to the purposes of claim 25 or 26, described preparation is to be dispersed in the air by heating and/or spray pattern.
28. the preparation method of each described preparation in the claim 1-24:
Described method comprises the step of extracting each described component in the described compound volatile oil.
29. preparation method according to claim 28:
Described method comprises the step of each described component in the described compound volatile oil of following extraction:
Adopt steam distillation from the raw material of each component, to extract each component, and/or adopt supercritical fluid extraction from the raw material of each component, to extract each component.
30. according to claim 28 or 29 described preparation methoies, described method also further comprises:
Measure each component of being extracted respectively;
With its mixing, optional stirring evenly, to obtain described compound volatile oil.
31. according to the preparation method of claim 30, described method is further comprising the steps of:
Measure described substrate, the optional substrate that multicomponent is formed is mixed, optional again stirring evenly;
And the mixture that described substrate is added described each volatile oil to be to mix, and optional mixture with volatile oil and substrate stirs evenly, and described disinfectant preparation with fresh air.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310283575.4A CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
| CN200710162111.2A CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
| HK14105895.9A HK1192416A1 (en) | 2007-12-14 | 2014-06-20 | Air fresh disinfectant |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710162111.2A CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
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|---|---|---|---|
| CN201310283575.4A Division CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
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| Publication Number | Publication Date |
|---|---|
| CN101455850A true CN101455850A (en) | 2009-06-17 |
| CN101455850B CN101455850B (en) | 2014-02-12 |
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| CN201310283575.4A Active CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
| CN200710162111.2A Active CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310283575.4A Active CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
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| HK (1) | HK1192416A1 (en) |
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| WO2010094151A1 (en) * | 2009-02-20 | 2010-08-26 | 永正顾问有限公司 | Disinfectant making air fresh and preparation method and use thereof |
| CN103393752A (en) * | 2012-07-30 | 2013-11-20 | 成都中医药大学 | New application of palchouli oil |
| CN103891738A (en) * | 2012-12-31 | 2014-07-02 | 青岛锦涟鑫商贸有限公司 | Indoor air freshening disinfectant |
| CN105412965A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Sandalwood air freshener and preparation method thereof |
| CN105412966A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Chinese herb air freshener and preparation method thereof |
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| CN105561363A (en) * | 2016-01-21 | 2016-05-11 | 广西大学 | Natural antibacterial air freshener and preparation method thereof |
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| CN108605999A (en) * | 2018-06-25 | 2018-10-02 | 杭州光启医疗科技发展有限公司 | A kind of medical bactericidal liquid and preparation method thereof |
| CN111493103A (en) * | 2020-05-21 | 2020-08-07 | 泓麟生物科技(广州)有限公司 | Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application |
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| CN103461394B (en) * | 2007-12-14 | 2015-07-22 | 永正顾问有限公司 | Air refreshing disinfectant |
| CN104996503B (en) * | 2015-07-22 | 2017-09-29 | 四川农业大学 | It is a kind of for Chinese medicine compound prescription air sterillization spray of plant and preparation method thereof |
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| CN1128672A (en) * | 1995-02-11 | 1996-08-14 | 卞伟 | Agent for purifying and refreshing air |
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- 2007-12-14 CN CN200710162111.2A patent/CN101455850B/en active Active
-
2014
- 2014-06-20 HK HK14105895.9A patent/HK1192416A1/en unknown
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| WO2010094151A1 (en) * | 2009-02-20 | 2010-08-26 | 永正顾问有限公司 | Disinfectant making air fresh and preparation method and use thereof |
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| CN105477662A (en) * | 2015-11-30 | 2016-04-13 | 陶超杰 | Jojoba oil air freshener and preparation method thereof |
| CN105412966A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Chinese herb air freshener and preparation method thereof |
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| CN107343865A (en) * | 2017-08-03 | 2017-11-14 | 成都煜泉绿健科技有限公司 | A kind of compound volatile oil and preparation method thereof, application |
| CN107343865B (en) * | 2017-08-03 | 2020-09-01 | 成都煜泉绿健科技有限公司 | Compound volatile oil and preparation method and application thereof |
| CN108605999A (en) * | 2018-06-25 | 2018-10-02 | 杭州光启医疗科技发展有限公司 | A kind of medical bactericidal liquid and preparation method thereof |
| CN111493103A (en) * | 2020-05-21 | 2020-08-07 | 泓麟生物科技(广州)有限公司 | Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application |
| CN112205430A (en) * | 2020-09-07 | 2021-01-12 | 恩民医药科技有限公司 | Traditional Chinese medicine extract composition and preparation method and application thereof |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN103461394A (en) | 2013-12-25 |
| CN103461394B (en) | 2015-07-22 |
| CN101455850B (en) | 2014-02-12 |
| HK1192416A1 (en) | 2014-08-22 |
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