CN101455850B - Air disinfectant - Google Patents
Air disinfectant Download PDFInfo
- Publication number
- CN101455850B CN101455850B CN200710162111.2A CN200710162111A CN101455850B CN 101455850 B CN101455850 B CN 101455850B CN 200710162111 A CN200710162111 A CN 200710162111A CN 101455850 B CN101455850 B CN 101455850B
- Authority
- CN
- China
- Prior art keywords
- oil
- preparation
- volatile oil
- oleum
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
Most air refreshing disinfectant sold in the prior market contains an artificially synthesized chemical sterilizer, and can be harmful to human body when the human body is often exposed in the air containing chemical products. Therefore, the invention provides an air refreshing disinfectant, and in particular relates to an air refreshing disinfectant which is not chemically synthesized and takes natural materials as raw materials and traditional Chinese medicine volatile oil as a main effective ingredient. The air refreshing disinfectant has the characteristics of bacteriostasis, antivirus, permanent aroma and economy and applicability, and is favorable for human health. In order to achieve the aims, the invention utilizes the characteristics of bacteriostasis, antivirus and permanent aroma of the traditional Chinese medicine volatile oil, and particularly adopts specified compositions of oleum eucalypti, clove oil, patchouli oil, forsythia oil and nepeta oil as active ingredients of the air refreshing disinfectant.
Description
Technical field
The present invention relates to a kind of air disinfectant, particularly a kind of with non-chemically synthetic, the air disinfectant that the volatile oil of the Traditional-Chinese-medicine-type that natural material is raw material is main effective ingredient.
Technical background
Environment goes from bad to worse, and in air, harmful substance increases, and abnormal flavour, aerial infection affect people's emotion, health, work efficiency.Cold, influenza or upper respiratory tract infection are commonly encountered diseases and the frequently-occurring diseases of winter-spring season, and it is propagated mainly by the spittle and air cross infection, and especially, in the crowd is dense, air is difficult for circulation local ，Ru hospital, school, company etc., incidence rate is very high.This is mainly owing to there being more pathogenic microorganism in the air of place, as influenza virus, rhinovirus, respiratory syncytial virus, adenovirus, Hemolytic streptococcus, staphylococcus, escherichia coli etc.Therefore, air being carried out disinfection is to prevent and control one of important measures of this cross infection.In order to kill airborne harmful microorganism and to remove abnormal flavour, air disinfectant is often used in Modern Family, hospital, hotel, restaurant and some other public arena in air.But the air disinfectant of selling in the market, contains the chemosterilant of synthetic mostly, is often exposed in this air that contains chemicals, may know from experience and cause certain injury people.Therefore, for purifying the air of a room, develop the pure and fresh sterile products of natural air safely and effectively, seem particularly important.
Summary of the invention
The technical deficiency that the present invention is directed to existing with fresh air and disinfectant etc., disclose the air disinfectant that a kind of volatile oil of take Traditional-Chinese-medicine-type is main effective ingredient, has antibacterial, antiviral, fragrant lasting, economic and practical feature, is of value to the health of human body.
For the obvious prescription of screening effect, selected staphylococcus aureus, Staphylococcus albus and three kinds of bacterial strains of escherichia coli and influenza A virus FM1 strain to carry out the evaluation of the antibacterial and antivirus action of this patent product.In common pathogenic bacteria and virus, staphylococcus, Bacterium entericum and influenza virus are antibacterial or the virus with important medical significance; These antibacterials or virus are distributed widely in nature, on air, soil, water and article, be also present in humans and animals skin and the tract that communicates with the external world in; In crowd, have 20%～50% people to carry pathogenic staphylococcus, medical worker's carrying rate can be up to 70%; Staphylococcus aureus and escherichia coli are one of main 6 kinds of antibacterials that cause hospital infection, and wherein coli-infection rate is classified first place as.Ministry of Health of China < < disinfection technology standard > > (version in 2002) clearly stipulates, in disinfectant germicidal experiment, its microorganism comprises staphylococcus aureus, Staphylococcus albus and escherichia coli etc., and the strain of air sterilization effect qualification test regulation is Staphylococcus albus.In addition, in first, second, influenza virus C, influenza A virus is the most popular.Therefore, this patent is chosen has three kinds of antibacterials such as staphylococcus aureus, Staphylococcus albus and escherichia coli of the meaning of representing and influenza A virus FM1 virus is antibacterial for following examples and the evaluation of antivirus action, and its result can reflect that patented product is used for air sterillization and truth and practical value when pure and fresh.
As mentioned above, the present invention is directed to the technical deficiency of existing with fresh air and disinfectant etc., disclose a kind of air disinfectant that traditional medicine volatile oil is main effective ingredient of take, there is antibacterial, antiviral, lasting, the economic and practical feature of fragrance, be of value to the health of human body.
For reaching this object, the present invention proposes the particular combination of a kind of Oleum Eucalypti, Oleum Caryophylli, patchouli oil, Fructus Forsythiae oil and Herba Schizonepetae oil as the active component of air disinfectant.
Above-mentioned composition of the present invention optionally contains substrate.In the situation that containing substrate, the ratio of substrate and volatile oil can change in wider scope, and impact effect not.
And be not particularly limited for operable substrate, as long as it can coordinate reaching of the object of the invention.
Above-mentioned air disinfectant can be dispersed in air by modes such as heating, sprayings, or with this air disinfectant filtration, rinse air and reach the object of sterilization with fresh air.
Compared with the prior art, tool of the present invention has the following advantages:
1, there is antibacterial and antivirus action, fragrant odour, fresh air, elimination abnormal flavour, and can bring fresh and comfortable, all there sensation to user.Can be widely used in the places such as family, office, commercial activity, hotel, compartment, particularly be applied to hospital, not only make people clear-headed, comfortable, and can greatly reduce the chance of infection.
2, product property is stable, and durable in use, Costco Wholesale Di， consumer easily accepts.
3, investment of production is few, workable.This product processes is simple, ripe, and equipment investment is few, easy to operate.
4, prescription is comprised of traditional medicine volatile oil, and product safety is reliable.
Specifically, technical scheme provided by the invention is as follows:
1. a preparation, it contains compound volatile oil, and described volatile oil is Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
2,, according to the preparation described in technical scheme 1, it is disinfectant preparation with fresh air.
3. according to the preparation described in technical scheme 1 or 2, it is characterized in that described preparation also contains substrate.
4. according to the preparation described in technical scheme 1 or 2, wherein said preparation is comprised of Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
5,, according to the described preparation of one of technical scheme 1-4, wherein Oleum Eucalypti is that myrtle Eucalyptus globulus Labill Eucalyptus globulus Labill., canella Camphor tree Cinnam omum camphora (L.) Presl or above-mentioned two sections belong to other plant together through extracting the volatile oil obtaining.
6,, according to the described preparation of one of technical scheme 1-5, wherein Fructus Forsythiae oil is that the dry fruit of Oleaceae plants Fructus Forsythiae Forsythia suspensa (Thunb.) Vahl is through extracting the volatile oil obtain.
7,, according to the described preparation of one of technical scheme 1-6, wherein Oleum Caryophylli is that the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb. is through extracting the volatile oil obtain.
8,, according to the described preparation of one of technical scheme 1-7, wherein patchouli oil is the dry stem of labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth. and the volatile oil that Ye Jing extraction obtains.
9,, according to the described preparation of one of technical scheme 1-8, wherein Herba Schizonepetae oil is that the herb of labiate Herba Schizonepetae Schizonepeta tenuifolia Briq. is through extracting the volatile oil obtain.
10,, according to the described preparation of one of technical scheme 1-9, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti approximately 6～90%, Fructus Forsythiae oil approximately 3～70%, Oleum Caryophylli approximately 5～80%, patchouli oil approximately 1～70%, Herba Schizonepetae oil approximately 1～85%.
11, according to the described preparation of one of technical scheme 1-10, the cumulative volume of compound volatile oil each component based on described compound volatile oil wherein, by volume part proportioning is as follows: Oleum Eucalypti approximately 21～71%, Fructus Forsythiae oil approximately 8～48%, Oleum Caryophylli approximately 15～60%, patchouli oil approximately 3～47%, Herba Schizonepetae oil approximately 3～53%.
12, according to the described preparation of one of technical scheme 1-11, the cumulative volume of compound volatile oil each component based on described compound volatile oil wherein, by volume part proportioning is as follows: Oleum Eucalypti approximately 18～63%, Fructus Forsythiae oil approximately 5～19%, Oleum Caryophylli approximately 6～27%, patchouli oil approximately 6～16%, Herba Schizonepetae oil approximately 6～29%.
13,, according to the described preparation of one of technical scheme 1-12, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti approximately 43～52%, Fructus Forsythiae oil approximately 13～20%, Oleum Caryophylli approximately 21～30%, patchouli oil approximately 7～15%, Herba Schizonepetae oil approximately 7～15%.
14,, according to the described preparation of one of technical scheme 1-13, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti approximately 45%, Fructus Forsythiae oil approximately 14%, Oleum Caryophylli approximately 23%, patchouli oil approximately 9%, Herba Schizonepetae oil approximately 9%.
15,, according to the described preparation of one of technical scheme 1-14, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is approximately 10: 3: 1: 1: 1.
16,, according to the described preparation of one of technical scheme 1-15, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is approximately 15: 4.5: 7.5: 3: 7.
17,, according to the described preparation of one of technical scheme 3-16, its mesostroma is the mixture of kerosene, isopropyl alcohol, dehydrated alcohol or its any combination.
18, according to preparation described in technical scheme 17, the cumulative volume of its mesostroma each component based on described substrate, by volume part proportioning is as follows:
Kerosene approximately 50～90%,
Isopropyl alcohol or dehydrated alcohol approximately 10～50%.
19,, according to preparation described in technical scheme 17, its mesostroma each component by volume part proportioning is as follows:
Kerosene approximately 71.4%,
Isopropyl alcohol or dehydrated alcohol approximately 28.6%.
20. according to the preparation of one of technical scheme 3-19, it is characterized in that described preparation is comprised of compound volatile oil and substrate.
21,, according to preparation described in technical scheme 20, wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil approximately 1～100%, substrate approximately 0～99%.
22,, according to preparation described in technical scheme 20, wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil approximately 1～10%, substrate approximately 90～99%.
23,, according to preparation described in technical scheme 22, wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil approximately 2.5%, substrate approximately 97.5%.
24,, according to preparation described in technical scheme 22, wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil approximately 10%, substrate approximately 90%.
25, in technical scheme 1-24 arbitrary described preparation for making the purposes of disinfecting air with fresh air.
26. according to the purposes of technical scheme 25, and it is dispersed in air, thus filtration, rinse air and reach the object of sterilization with fresh air.
27. according to the purposes of technical scheme 25 or 26, and described preparation is to be dispersed in air by heating and/or spray pattern.
The preparation method of preparation described in any one in 28. technical scheme 1-24:
Described method comprises the step of extracting in described compound volatile oil component described in each.
29. according to the preparation method described in technical scheme 28:
Described method comprises in the described compound volatile oil of following extraction the step of component described in each:
Adopt steam distillation from the raw material of each component, to extract each component, and/or adopt supercritical fluid extraction from the raw material of each component, to extract each component.
30. according to the preparation method described in technical scheme 28 or 29, and described method also further comprises:
Measure respectively each extracted component;
Mixed, optionally stirred evenly, to obtain described compound volatile oil.
31. according to the preparation method of technical scheme 30, and described method is further comprising the steps of:
Measure described substrate, the substrate optionally multicomponent being formed is mixed, more optionally stirs evenly;
And described substrate is added the mixture of described each volatile oil to mix, optionally the mixture of volatile oil and substrate is stirred evenly, and obtain described disinfectant preparation with fresh air.
Accompanying drawing explanation
Fig. 1 is the sampled point plane graph of the embodiment of field experimentation.Reference numeral 1 is sampled point.
The specific embodiment
In this article, the implication of " pure and fresh sterilization " is to instigate air present natural aromatic odor and supression and/or kill airborne common bacteria and/or virus.
" Oleum Eucalypti, Oleum Caryophylli, patchouli oil, Fructus Forsythiae oil and Herba Schizonepetae oil " herein forms, and is also referred to as compound volatile oil, herbal mixture volatilization wet goods.
How to prepare in the explanation of each sample below, in compound volatile oil, the raw material of each component is:
Oleum Eucalypti: general Oleum Eucalypti all can be used, a concrete example is to belong to other plant together through extracting the volatile oil obtaining from myrtle Eucalyptus globulus Labill Eucalyptus globulus Labill., canella Camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections.(commercial goods of using in embodiment described herein is purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, and lot number is 20060825.)
Fructus Forsythiae oil, general Fructus Forsythiae oil all can be used, and a concrete example is through extracting the volatile oil obtaining from the dry fruit of Oleaceae plants Fructus Forsythiae Forsythia suspensa (Thunb.) Vahl..(commercial goods of using in embodiment described herein is purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, and lot number is 20060825.)
Oleum Caryophylli, general Oleum Caryophylli all can be used, and a concrete example is through extracting the volatile oil obtaining from the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb..(commercial goods of using in embodiment described herein is purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, and lot number is 20060825.)
Patchouli oil, general patchouli oil all can be used, and a concrete example is to extract from dry stem and the Ye Jing of labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth. the volatile oil obtaining.(commercial goods of using in embodiment described herein is purchased from Guangzhou pot-pourri company limited, and lot number is 20060901.)
Herba Schizonepetae oil, general Herba Schizonepetae oil all can be used, and a concrete example is through extracting the volatile oil obtaining from the herb of labiate Herba Schizonepetae Schizonepeta tenuiflia Briq..(commercial goods of using in embodiment described herein is purchased from Jishui County, Jiangxi Province Gan Xu perfumery oil factory, and lot number is 20060825.)
Except buying commercially available prod, also can adopt steam distillation or supercritical fluid extraction to extract according to a conventional method above-mentioned volatile oil from Chinese crude drug, or also can adopt other existing known extracting method to extract.
Above-mentioned Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil also can extract respectively from the plant of other section, genus.
Wherein Oleum Eucalypti, patchouli oil are respectively by carrying out quality monitoring under the 280th, 272 pages of items of 2005 editions Firsts of < < Chinese Pharmacopoeia > >, Oleum Caryophylli, by carrying out quality monitoring under the 7th page of item of 1953 editions Firsts of < < Chinese Pharmacopoeia > >, is set up voluntarily the quality standard of Fructus Forsythiae oil, Herba Schizonepetae oil and is monitored.
Be non-limiting example of the present invention below, below formula (unless specifically stated otherwise) is all parts by volume proportioning.
The bacteriostasis of single and herbal mixture volatile oil (antibacterial around-France)
Sample:
Sample 1: Oleum Eucalypti
Sample 2: Fructus Forsythiae oil
Sample 3: patchouli oil
Sample 4: Oleum Caryophylli
Sample 5: Herba Schizonepetae oil
Sample 6: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 10: 3: 1: the mixture of 1: 1 ratio composition.
Preparation method: measure respectively Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml, mix, stir evenly, obtain.
Experimental strain:
Staphylococcus aureus (26112), escherichia coli (44113), Staphylococcus albus (26069), by Beijing, Nat'l Pharmaceutical & Biological Products Control Institute provides.
Test method:
By 16～18 hours liquid cultures of above-mentioned three kinds of bacterium, with aseptic cotton carrier, evenly coat respectively ordinary flat surface, slightly dry, with aseptic nipper gripping, inhale the drug sensitive test paper that has quantitative sample medicinal liquid, be laid in the planar surface of above-mentioned inoculated bacteria, a flat board is put 3 drug sensitive test papers, puts 37 ℃ of cultivations, next day observed result.Result of the test is in Table 1.
Table 1 single medicinal material volatile oil fungistatic effect result of the test (inhibition zone diameter, mm, n=2～5,
)
Note:
Comprehensive antibacterial index, D=A * 0.3+B * 0.2+C * 0.5, lower same.
N represents to test number of repetition (lower same).
Result shows, Oleum Eucalypti, Fructus Forsythiae oil, patchouli oil, Oleum Caryophylli, Herba Schizonepetae oil all have inhibitory action in various degree to staphylococcus aureus, Staphylococcus albus, colibacillary growth.Wherein Oleum Caryophylli is the strongest to the effect of staphylococcus aureus, and Herba Schizonepetae oil is the strongest to colibacillary effect.
Owing to having used three kinds of antibacterials, and sample is different to the action intensity of different bacterium, be difficult to the whole fungistatic effect to the inhibition assess sample of three kinds of antibacterials by simple more different samples, therefore need carry out overall merit, this patent adopts composite index law to evaluate.
Composite index law is a kind of conventional comprehensive analysis method, by giving sample the weight different to the fungistatic effect of three kinds of antibacterials, will consider that the fungistatic effect after weight is simply added, and draws a comprehensive antibacterial index (computing formula is in Table 1 footnote).The size of weight is to determine according to the importance of each factor and/or to result contribution, herein because coccus represents respectively with bacillus the antibacterial that two classes are different, so the weight of coccus and bacillus all gives 0.5; Because coccus has been used two kinds, wherein staphylococcus aureus pathogenicity is stronger, therefore give two kinds of coccus weights, is respectively 0.3 and 0.2.Result demonstration, the comprehensive antibacterial index of sample 6 (herbal mixture volatile oil) is the highest, shows that the fungistatic effect of herbal mixture volatile oil is better than single volatile oil, and reasonability and the superiority of prompting compound recipe, reflected its novelty and creativeness.
In order further to understand after single volatile oil forms compound recipe, have or not chemiluminescence, according to each single medicinal material volatile oil proportion in fungistatic effect separately of single volatile oil and sample 6, the comprehensive antibacterial index of the theory of computation, result is 10.1.This is starkly lower than the comprehensive antibacterial index 15.0 of sample 6 reality, prompting single volatile oil compound recipe forms the rear mutual synergism having produced of sample 6, fungistatic effect is strengthened, this has confirmed the conclusion that the direct comparison of the preceding paragraph by the comprehensive antibacterial index of actual measurement of compound recipe and single volatile oil draws, novelty and the creativeness of compound recipe has been described.
Embodiment 2
The bacteriostasis (antibacterial around-France) of different proportioning herbal mixture volatile oil
Sample:
Sample 7: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 5: 1.5: 2.5: the mixture of 2: 3 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 357.1ml, Fructus Forsythiae oil 107.1ml, Oleum Caryophylli 178.6ml, patchouli oil 142.9ml, Herba Schizonepetae oil 214.3ml, mix, stir evenly, obtain.
Sample 8: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 5: 3: 5: the mixture of 3: 5 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 238.1ml, Fructus Forsythiae oil 142.9ml, Oleum Caryophylli 238.1ml, patchouli oil 142.9ml, Herba Schizonepetae oil 238.1ml, mix, stir evenly, obtain.
Sample 9: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 5: 4.5: 7.5: the mixture of 4: 7 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 178.6ml, Fructus Forsythiae oil 160.7ml, Oleum Caryophylli 267.9ml, patchouli oil 142.9ml, Herba Schizonepetae oil 250.0ml, mix, stir evenly, obtain.
Sample 10: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 10: 1.5: 2.5: the mixture of 3: 7 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 416.7ml, Fructus Forsythiae oil 62.5ml, Oleum Caryophylli 104.2ml, patchouli oil 125.0ml, Herba Schizonepetae oil 291.7ml, mix, stir evenly, obtain.
Sample 11: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 10: 3: 5: the mixture of 4: 3 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 400.0ml, Fructus Forsythiae oil 120.0ml, Oleum Caryophylli 200.0ml, patchouli oil 160.0ml, Herba Schizonepetae oil 120.0ml, mix, stir evenly, obtain.
Sample 12: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 10: 4.5: 7.5: the mixture of 2: 5 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 344.8ml, Fructus Forsythiae oil 155.2ml, Oleum Caryophylli 258.6ml, patchouli oil 69.0ml, Herba Schizonepetae oil 172.4ml, mix, stir evenly, obtain.
Sample 13: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 15: 1.5: 2.5: the mixture of 4: 5 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 535.7ml, Fructus Forsythiae oil 53.6ml, Oleum Caryophylli 89.3ml, patchouli oil 142.9ml, Herba Schizonepetae oil 178.6ml, mix, stir evenly, obtain.
Sample 14: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 15: 3: 5: the mixture of 2: 7 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 468.8ml, Fructus Forsythiae oil 93.8ml, Oleum Caryophylli 156.3ml, patchouli oil 62.5ml, Herba Schizonepetae oil 218.8ml, mix, stir evenly, obtain.
Sample 15: Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil were in 15: 4.5: 7.5: the mixture of 3: 3 ratio compositions.
Preparation method: measure respectively Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml, mix, stir evenly, obtain.
The bacteriostasis of measuring above-mentioned sample by the method for embodiment 1, result of the test is in Table 2.
The different proportioning Chinese medicine compound of table .2 volatile oil bacteriostatic test result (inhibition zone diameter, mm, n=3,
)
(ratio that contains in embodiment sample 7～15 of each volatile oil is, Oleum Eucalypti 17.9～53.6%, Fructus Forsythiae oil 5.4～16.1%, Oleum Caryophylli 8.9～26.8%, patchouli oil 6.3～16.0%, Herba Schizonepetae oil 9.1～29.2%.)
Result shows, the product of above-mentioned 9 formulas all has fungistatic effect.Calculate the antibacterial index of synthesis in theory of each formula, be respectively: 114,11.7,11.8,11.5,10.9,11.6,10.9,11.5,11.1, all lower than the comprehensive antibacterial index of actual measurement of each correspondence (D row in table 2), illustrate that 9 formulas have all produced synergism by compatibility aspect antibacterial, so the prescription of these 9 samples has novelty and creativeness too.
In 9 formulas, sample 7,11,12,13,14 has fungistatic effect, and sample 8,9,10 has good fungistatic effect, and sample 15 fungistatic effects are best.In sample 15, the ratio of each volatile oil is: Oleum Eucalypti 45.5%, Fructus Forsythiae oil 13.6%, Oleum Caryophylli 22.7%, patchouli oil 9.1%, Herba Schizonepetae oil 9.1%.
Above-mentioned test is by volatile oil different proportion orthogonal design L
9(3
4) and the result that obtains is further analyzed known according to orthogonal design to its result: the ratio of Oleum Eucalypti in formula changes, little on fungistatic effect impact; The ratio of Fructus Forsythiae oil, Oleum Caryophylli and Herba Schizonepetae oil changes has considerable influence to fungistatic effect, fungistatic effect is along with their ratio increases and increases, the ratio of patchouli oil has the greatest impact to fungistatic effect, and during the ratio 6.3～16.0% changes, fungistatic effect is first to increase rear reduction.According to the best ratio of the drawn theoretic fungistatic effect of the information of above-described embodiment, it is 15: 4.5: 7.5: 3: 7, Oleum Eucalypti 40.5%, Fructus Forsythiae oil 12.2%, Oleum Caryophylli 20.3%, patchouli oil 8.1%, Herba Schizonepetae oil 18.9%.
Optimal proportion and above-mentioned " ratio of Oleum Eucalypti in formula changes, little on fungistatic effect impact thus; The ratio of Fructus Forsythiae oil, Oleum Caryophylli and Herba Schizonepetae oil changes has considerable influence to fungistatic effect, and fungistatic effect is along with their ratio increases and increases " discovery, can reason out, the content range of each volatile oil component can be at following range changing:
Oleum Eucalypti 43～52%, Fructus Forsythiae oil 13～20%, Oleum Caryophylli 21～30%, patchouli oil 7～15%, Herba Schizonepetae oil 7～15%; Or
Oleum Eucalypti 18～63%, Fructus Forsythiae oil 5～19%, Oleum Caryophylli 6～27%, patchouli oil 6～16%, Herba Schizonepetae oil 6～29%, or Oleum Eucalypti 21～71%, Fructus Forsythiae oil 8～48%, Oleum Caryophylli 15～60%, patchouli oil 3～47%, Herba Schizonepetae oil 3～53%;
Or
Oleum Eucalypti 6～90%, Fructus Forsythiae oil 3～70%, Oleum Caryophylli 5～80%, patchouli oil 1～70%, Herba Schizonepetae oil 1～85%.
Explanation for above-mentioned scope:
Wherein, because the ratio of Oleum Eucalypti in formula changes, little on fungistatic effect impact, the variation of the content upper and lower limit of Oleum Eucalypti can be very large; And the ratio of Fructus Forsythiae oil, Oleum Caryophylli and Herba Schizonepetae oil changes, near the content according to embodiment, make legitimate inference, consider that its fungistatic effect is along with their ratio increases and the fact that increases, thus its upper limit actual can be than in embodiment larger.
In addition, because the feasible content of each component variation within the scope of in formula is successional, any combination between the end points of the broad scope that therefore the disclosed point of embodiment and above-mentioned plaintext are recorded is also feasible, also belongs to the clear and definite scope of disclosure of the present invention.Equally, any coupling between any content range of a component and any content range of other any component is also feasible, belongs to the clearly disclosed part of the present invention.
Embodiment 3
The bacteriostasis (antibacterial around-France) of different proportioning herbal mixture volatile oil
Sample:
Sample 6: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (10: 3: 1: 1: 1)
Preparation method: measure respectively Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml, mix, stir evenly, obtain.
Sample 15: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15: 4.5: 7.5: 3: 3)
Preparation method: measure respectively Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml, mix, stir evenly, obtain.
Sample 16: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15: 4.5: 7.5: 3: 7)
Preparation method: measure respectively Oleum Eucalypti 405.4ml, Fructus Forsythiae oil 121.6ml, Oleum Caryophylli 202.7ml, patchouli oil 81.1ml, Herba Schizonepetae oil 189.2ml, mix, stir evenly, obtain.
The bacteriostasis of measuring above-mentioned sample by the method for embodiment 1, result of the test is in Table 3.
The different proportioning sample of table 3 traditional medicine volatile oil inhibition zone result of the test (inhibition zone diameter, mm, n=3,
)
Result shows, the product of above-mentioned 3 formulas all has fungistatic effect.The optimization of C/C composites of sample 16 for calculating according to the result in example 2, result shows that its fungistatic effect is better.Calculate the antibacterial index 11.5 of its synthesis in theory, lower than the comprehensive antibacterial index 14.3 of its actual measurement, further proved that compatibility of drugs produces the synergism that curative effect strengthens; Sample 16 formulas have novelty and creativeness.
Embodiment 4
The bacteriostasis (tube dilution method) of different proportioning herbal mixture volatile oil
Sample:
Sample 6: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (10: 3: 1: 1: 1)
Preparation method: measure respectively Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml, mix, stir evenly, obtain.
Sample 15: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15: 4.5: 7.5: 3: 3)
Preparation method: measure respectively Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml, mix, stir evenly, obtain.
Sample 16: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15: 4.5: 7.5: 3: 7)
Preparation method: measure respectively Oleum Eucalypti 405.4ml, Fructus Forsythiae oil 121.6ml, Oleum Caryophylli 202.7ml, patchouli oil 81.1ml, Herba Schizonepetae oil 189.2ml, mix, stir evenly, obtain.
Experimental technique:
Add respectively 1ml Tween 80 to make its dissolving sample 6,15,16 each 3ml, add again nutrient broth to be mixed with every ml and be respectively 0.30ml, 0.15ml, 0.075ml, 0.0375ml, 0.01875ml, 0.009375ml, 0.004688ml, 0.002344ml, 0.001172ml containing crude drug amount, the dilute sample of totally 9 drug level, every pipe total amount 1ml, heat sterilization.
Control tube: strain contrast, for not containing the culture medium Accelerated Life bacterium of medicine; Medicine contrast is the medicinal liquid of Accelerated Life bacterium not.
It is 10 that each concentration pipe of every drug row liquid and strain control tube add respectively concentration
6cfuml
-1test organisms liquid (8 hours cultures) 0.1ml, cultivate 24 hours for 37 ℃, then each concentration got to a ring streak inoculation on solid nutrient agar panel with inoculating loop, cultivate 24 hours observed results for 37 ℃.The turbidity of take has asepsis growth as each pipe of index naked-eye observation; With the naked eye observe each flat board have or not colony growth.
Judge minimal inhibitory concentration (MIC), refer to the minimum drug level that complete inhibition test growth is contained.
Judge minimal bactericidal concentration (MBC), refer to kill completely the test strain contained minimum drug level of growing.
Result of the test is in Table 4.
The different proportioning herbal mixture of table 4 volatile oil is to the MIC of 3 kinds of antibacterials and MBC measurement result (μ lml
-1)
MIC and MBC are two important indicators of the antibacterial and sterilization of medicine, and its antibacterial effect that is worth the bright medicine of novel is more better, utilizes this two parameters, and the concentration of sample 6,15,16 and its fungistatic effect are evaluated.Result shows, above-mentioned 3 samples all have Antibacteria effect in various degree to test strain, and the MIC of each strain is between 2.34 μ lml
-1～75.0 μ lml
-1between, the MBC of each strain is between 4.69 μ lml
-1～750 μ lml
-1between, wherein the comprehensive bactericidal index of the MBC of sample 16 is minimum, and approaching of its MIC and sample 15 illustrates that its comprehensive bactericidal effect is best, and comprehensive fungistatic effect is also better.
Embodiment 5
The antivirus action of different proportioning herbal mixture volatile oil
Sample:
Sample 6: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (10: 3: 1: 1: 1)
Preparation method: measure respectively Oleum Eucalypti 625.0ml, Fructus Forsythiae oil 187.5ml, Oleum Caryophylli 62.5ml, patchouli oil 62.5ml, Herba Schizonepetae oil 62.5ml, mix, stir evenly, obtain.
Sample 15: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15: 4.5: 7.5: 3: 3)
Preparation method: measure respectively Oleum Eucalypti 454.5ml, Fructus Forsythiae oil 136.4ml, Oleum Caryophylli 227.3ml, patchouli oil 90.9ml, Herba Schizonepetae oil 90.9ml, mix, stir evenly, obtain.
Sample 16: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil (15: 4.5: 7.5: 3: 7)
Preparation method: measure respectively Oleum Eucalypti 405.4ml, Fructus Forsythiae oil 121.6ml, Oleum Caryophylli 202.7ml, patchouli oil 81.1ml, Herba Schizonepetae oil 189.2ml, mix, stir evenly, obtain.
Positive control drug virazole, lot number 0301006, by Hubei, medical professionals is provided.
Seed culture of viruses:
Influenza A virus FM1Zhu You Inst. of Medicinal Biological Technology, Chinese Academy of Medical Sciences provides, in MDCK Madin-Darby canine kidney(cell line) (MDCK) (Madin-Darby Canine Kidney cell, Madin-Darby canine kidney cell), go down to posterity, with pathological changes and and chicken erythrocyte Hemagglutination Method judge its titre, at 37 ℃, 5%CO
2in incubator, cultivate.
Experimental technique:
Press < < study of tcm new drug guide > > (bureau of drug policy & administration of Ministry of Health of the People's Republic of China, version in 1994) require to carry out, above virus test is carried out on 96 hole micro plates, every hole 0.1ml.Cell number is 1 * 10
5individual/ml grows up to after monolayer, and in inciting somebody to action, medicinal maintenance medium is diluted to 4 variable concentrations, after jettisoning original fluid, variable concentrations traditional Chinese medicine sample is added in cell hole, and every hole 0.1ml, the cell of take does not occur that the minimum dilution factor of pathological changes is as this medicinal liquid is to the nontoxic boundary of cell.On mdck cell, measure the TCID of challenge virus simultaneously
50(50%tissue culture infective dose, tissue culture infective dose).During test, establish virus control, cell contrast, drug toxicity contrast and positive drug contrast (virazole).Put 37 ℃, 5%CO
2in incubator, cultivate.Under inverted microscope, observe pathological changes record every day.When virus control porocyte occurs +++ during pathological changes, continue to observe stopping for 7 days.The degree that pathological changes appears in cell is by 6 grade standard records :-: Growth of Cells is normal, without pathological changes, occurs; " ± ": cytopathy is less than 10% of whole cell monolayer; "+": cytopathy is less than 25% of whole cell monolayer; " ++ ": cytopathy is less than 50% of whole cell monolayer; " +++ ": cytopathy is less than 75% of whole cell monolayer; " ++++": cytopathy accounts for the more than 75% of whole cell monolayer.Result of the test is in Table 5.
The different proportioning samples of table 5 traditional medicine volatile oil are to influenza A virus FM1 inhibitory action
Note: "+" suppresses "-" to viral unrestraint effect completely to virus
Result shows, is infecting viral TCID
50under 100 dosage, virazole 100 μ gml
-1fM1 is had to obvious inhibitory action, and under inferior toxicity concentration, No. 15, sample is at 160 μ lml
-1fM1 is had to certain inhibitory action.Interpret sample 15 also has the effect that suppresses airborne common virus, has the effect of air sterillization.(in embodiment subsequently, selecting the formula of sample 15 to carry out the object that one of them continues research)
Embodiment 6
Simulated field test
Sample:
Chinese medicine air disinfectant 1: formulated by 1: 9 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil be Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil by 15: 4.5: 7.5: form at 3: 3, substrate be kerosene with dehydrated alcohol by 2.5: 1 compositions.
Preparation method: measure respectively Oleum Eucalypti 45.5ml, Fructus Forsythiae oil 13.6ml, Oleum Caryophylli 22.7ml, patchouli oil 9.1ml, Herba Schizonepetae oil 9.1ml, mix, stir evenly, obtain herbal mixture volatile oil, standby; Measure respectively kerosene 642.9ml and dehydrated alcohol 257.1ml, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, obtain.
Equipment:
Test strain: Staphylococcus albus 8032 ，You Military Medical Science Institutes provide.Strain algebraically was 6th～8 generations, with ordinary broth, prepared bacteria suspension.
Aerosol generator, 90% above particle Φ≤5 μ m.
Aerosol chamber's volume is 10m
3.
Sampler: FA-1 type impacting type air microorganism sampler.
Method:
Test basis: Ministry of Public Health < < disinfection technology standard > > (version in 2002) 2.1.3.
23～24 ℃ of test temperatures, relative humidity 50～58%.
Microbiological contamination aerosol apparatus flow is 21.7L/min, and spray time 5min stirs 5min, standing 5min.
Sterilization method: during test with 2ml/m
3amount, with QPQ-2300 type electric motion aerosol atomizer, laboratory is sprayed, disinfective action 60min post-sampling.Matched group replaces disinfectant with deionized water, and other operate same test group.Test repeats 3 times.
Adopt FA-1 type impacting type air microorganism sampler, sampling flow is 28.3L/min, in chamber central authorities, apart from ground 1.0m, establishes a point sampling.When disinfection time is 0min, 60min, sample, the matched group sampler sampling time is respectively 5s, 5s, and the test group sampler sampling time is respectively 5s, 1min, measures different time and deposit viable count after sampling, is calculated as follows killing rate:
N
t: the natural decay rate of antibacterial in air;
V
0with V
t: be respectively matched group before on-test and the air bacteria containing amount of different time in process of the test;
K
1: disinfect the killing rate to antibacterial in air;
V
0' with V
t': be respectively test group disinfect before and the air bacteria containing amount of different time in disinfecting process.
The results are shown in Table 6.
1 pair of staphylococcic killing effect of white of table 6 Chinese medicine air disinfectant
Negative control: diluent, the equal asepsis growth of culture medium.
Result shows, Chinese medicine air disinfectant 1 disinfective action 60min, and to the staphylococcic killing rate of white, 3 test killing rates are all more than 96.30%.Illustrate that Chinese medicine air disinfectant 1 can kill airborne common bacteria, there is the effect of air disinfection.
Embodiment 7
Field trial
Sample:
Chinese medicine air disinfectant 2: formulated by 1: 39 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil be Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil by 15: 4.5: 7.5: form at 3: 3, substrate be kerosene with isopropyl alcohol by 2.5: 1 compositions.
Preparation method: measure respectively Oleum Eucalypti 11.4ml, Fructus Forsythiae oil 3.4ml, Oleum Caryophylli 5.7ml, patchouli oil 2.3ml, Herba Schizonepetae oil 2.3ml, mix, stir evenly, obtain herbal mixture volatile oil, standby; Measure respectively kerosene 696.4ml and isopropyl alcohol 278.6ml, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, obtain.
Chinese medicine air disinfectant 3: formulated by 1: 9 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil be Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil by 15: 4.5: 7.5: form at 3: 3, substrate be kerosene with isopropyl alcohol by 2.5: 1 compositions.
Preparation method: measure respectively Oleum Eucalypti 45.5ml, Fructus Forsythiae oil 13.6ml, Oleum Caryophylli 22.7ml, patchouli oil 9.1ml, Herba Schizonepetae oil 9.1ml, mix, stir evenly, obtain herbal mixture volatile oil, standby; Measure respectively kerosene 642.9ml and isopropyl alcohol 257.1ml, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, obtain.
Experimental enviroment and instrument:
Room volume: 70m
3
Temperature and humidity: room temperature: 31 ℃, room relative humidity: 85%
Spraying instrument: electric motion aerosol atomizer, model: DQP-600, producer: Beijing development in science and technology company limited of Song Yuan Huaxing
Sterilization and sampling:
Sampled point is as shown below, carries out altogether 2 samplings, when the sampling time, point was respectively 0h, during 2h.
The method of sampling: on 5 sampled points as shown in Figure 1, it is dull and stereotyped that each point is put two diameter 9cm, and height of sampling is 1.0m, opens culture dish, exposes after 10 minutes, builds lid, and sample, in 37 ℃ of cultivations 24 hours, calculates clump count and is also calculated as follows extinction rate.
Sterilizing operation: undertaken by the dosage of every cubic metre of 1ml, be evenly sprayed on room with electric motion aerosol atomizer, been sprayed, sterilization 2h.
Statistical Identifying Method:
The results are shown in Table 7,8.
Disinfection Effect under table 7 Chinese medicine air disinfectant 2 on-the-spot air-tight states
Disinfection Effect under table 8 Chinese medicine air disinfectant 3 on-the-spot air-tight states
Result shows, under air-tight state, with air in 2,3 pairs of spaces of Chinese medicine air disinfectant, carries out pure and fresh sterilization at the scene, can make bacterial number in air reduce approximately 71% and 76% after sterilization, reduces and has significant.Illustrate that Chinese medicine air disinfectant 2,3 can kill airborne antibacterial, there is the disappear effect of bacterium of air.
According to the data of above-described embodiment 1: 9 and 1: 39, and effect was visible, and the ratio of substrate and volatile oil can change in wider scope, and effect is not had to what impact.In following scope, all can implement the present invention:
Compound volatile oil > 0～≤ 100%, substrate 0～99%; Wherein " > 0 " represents to be greater than 0.
Compound volatile oil 1～100%, substrate 0～99%;
Or compound volatile oil 1～10%, substrate 90～99%.
And for the selection of operable substrate, be not particularly limited, as long as it can coordinate reaching of the object of the invention, this is that those skilled in the art can simply measure selection.The example of substrate includes but not limited to as kerosene, isopropyl alcohol, dehydrated alcohol etc., or the mixture of its any combination.
Embodiment 8
Acute oral toxicity test
Test objective:
Observe Chinese medicine air disinfectant and once give the toxic reaction producing after animal, evaluate the safety to human body when practical application of this pure and fresh disinfectant.
Sample:
Chinese medicine air disinfectant 2: formulated by 1: 39 by herbal mixture volatile oil and substrate, wherein herbal mixture volatile oil be Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil, Herba Schizonepetae oil by 15: 4.5: 7.5: form at 3: 3, substrate be kerosene with isopropyl alcohol by 2.5: 1 compositions.
Preparation method: measure respectively Oleum Eucalypti 11.4ml, Fructus Forsythiae oil 34.ml, Oleum Caryophylli 5.7ml, patchouli oil 2.3ml, Herba Schizonepetae oil 2.3ml, mix, stir evenly, obtain herbal mixture volatile oil, standby; Measure respectively kerosene 696.4ml and isopropyl alcohol 278.6ml, mix, stir evenly, add above-mentioned mixture of volatile oil, mix, stir evenly, obtain.
That during test, use is Jin Longyu
oleum Arachidis hypogaeae semen (the date of manufacture: 2007-01-30) (certainly also can using of other) dilution.
Experimental animal:
NIH (National Institute of Health, NIH) be white mice (quality certification number: 0027327), male and female half and half, body weight 18～22g，You Guangdong Province Experimental Animal Center provide.
Test method:
Getting NIH is 20 of mices, male and female half and half, body weight 18～22g.Animal adaptability is raised 2d.Animal overnight fasting before administration, freely drink water.During test, get appropriate Oleum Arachidis hypogaeae semen dilution 5.0287g Chinese medicine air disinfectant 2 to 20ml, by 20ml/kg body weight, it is gavage of dosage 5000mg/kg body weight, at once observe reaction of animals, Continuous Observation two weeks, record animal toxicity response situation and dead distribution, dead animal is performed an autopsy on sb． simultaneously, record pathological changes situation, judge LD
50whether be greater than 5000mg/kg body weight.
Result and conclusion:
Result of the test shows: Chinese medicine air disinfectant 2 is the disposable gavage of 5000mg/kg body weight according to dosage, and overt toxicity reaction does not appear in animal.After administration, the reaction main manifestations of animal is movable minimizing, and minority occurs excitement, beats; After administration, 2 hours animal activities recover normal substantially; Without animal, occur dead.Therefore judge its LD
50be greater than 5000mg/kg body weight.
According to the toxicity assessment standard in the < < of Ministry of Health of the People's Republic of China disinfection technology standard > > (version in 2002):
LD
50be greater than the true border of 5000mg/kg body severe one nontoxic;
LD
50for 501mg/kg～5000mg/kg body severe one belongs to low toxicity;
LD
50for 51mg/kg～500mg/kg body severe one belongs to medium poison;
LD
50for 1mg/kg～50mg/kg body severe one belongs to high poison;
LD
50be less than 1mg/kg body severe one and belong to severe toxicity.
LD due to this Chinese medicine air disinfectant 2
50be greater than 5000mg/kg body weight, can think and belong to nontoxic product.
Conclusion
Chinese medicine air disinfectant has antibacterial, sterilization significantly and antiviral activity even, it writes out a prescription novel, can obviously suppress the growth of air middle school common pathogen, there is not animal dead and obvious toxic reaction in the oral 5000mg/kg of animal, point out it safe and effective, its abnormal smells from the patient is pure and fresh, can be used for air sterillization and pure and fresh.
In addition, embodiment is herein all non-limiting example, and related numerical value, unless specialized, can be also about value.Any combination between the end points of the scope of the disclosed point of embodiment and summary is also scope disclosed by the invention; Any coupling between any content range of a component and any content range of other component is also a part disclosed by the invention.According to variation of the present invention, also belong to a part of the present invention.
Claims (27)
1. a disinfectant preparation with fresh air, it contains compound volatile oil, and described volatile oil is Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil;
Wherein, the each component of described compound volatile oil, the cumulative volume based on described compound volatile oil, by volume the proportioning of part is as follows:
Oleum Eucalypti 18～63%, Fructus Forsythiae oil 5～19%, Oleum Caryophylli 6～27%, patchouli oil 6～16%, Herba Schizonepetae oil 6～29%.
2. preparation according to claim 1, is characterized in that described preparation also contains substrate.
3. preparation according to claim 1, wherein said preparation is comprised of Oleum Eucalypti, Fructus Forsythiae oil, Oleum Caryophylli, patchouli oil and Herba Schizonepetae oil.
4. according to the described preparation of one of claim 1-3, wherein Oleum Eucalypti is that the other plant that belongs to together of myrtle Eucalyptus globulus Labill Eucalyptus globulus Labill., canella Camphor tree Cinnamomum camphora (L.) Presl or above-mentioned two sections is through extracting the volatile oil obtaining.
5. according to the described preparation of one of claim 1-3, wherein Fructus Forsythiae oil is that the dry fruit of Oleaceae plants Fructus Forsythiae Forsythia suspensa (Thunb.) Vahl is through extracting the volatile oil obtaining.
6. according to the described preparation of one of claim 1-3, wherein Oleum Caryophylli is that the dry flower of plant clove of myrtaceae Eugenia caryophyllata Thunb. is through extracting the volatile oil obtaining.
7. according to the described preparation of one of claim 1-3, wherein patchouli oil is the dry stem of labiate Herba Pogostemonis Pogostemon cablin (Blanco) Benth. and the volatile oil that Ye Jing extraction obtains.
8. according to the described preparation of one of claim 1-3, wherein Herba Schizonepetae oil is that the herb of labiate Herba Schizonepetae Schizonepeta tenuifolia Briq. is through extracting the volatile oil obtaining.
9. according to the described preparation of one of claim 1-3, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti 43～52%, Fructus Forsythiae oil 13～20%, Oleum Caryophylli 21～30%, patchouli oil 7～15%, Herba Schizonepetae oil 7～15%.
10. according to the described preparation of one of claim 1-3, wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti 45%, Fructus Forsythiae oil 14%, Oleum Caryophylli 23%, patchouli oil 9%, Herba Schizonepetae oil 9%.
11. according to the described preparation of one of claim 1-3, and wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is 10: 3: 1: 1: 1.
12. according to the described preparation of one of claim 1-3, and wherein by volume part proportioning is as follows for compound volatile oil each component: Oleum Eucalypti: Fructus Forsythiae oil: Oleum Caryophylli: patchouli oil: Herba Schizonepetae oil is 15: 4.5: 7.5: 3: 7.
13. preparations according to claim 2, its mesostroma is the mixture of kerosene, isopropyl alcohol, dehydrated alcohol or its any combination.
14. according to preparation described in claim 13, the cumulative volume of its mesostroma each component based on described substrate, and by volume part proportioning is as follows:
Kerosene 50～90%,
Isopropyl alcohol or dehydrated alcohol 10～50%.
15. according to preparation described in claim 13, and its mesostroma each component by volume part proportioning is as follows:
Kerosene 71.4%,
Isopropyl alcohol or dehydrated alcohol 28.6%.
16. according to the preparation of claim 2, it is characterized in that described preparation is comprised of compound volatile oil and substrate.
17. according to preparation described in claim 16, and wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil 1～100%, substrate 0～99%.
18. according to preparation described in claim 16, and wherein, based on described preparation cumulative volume, compound volatile oil and substrate by volume part proportioning are as follows: compound volatile oil 1～10%, substrate 90～99%.
19. according to preparation described in claim 18, and wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil 2.5%, substrate 97.5%.
20. according to preparation described in claim 18, and wherein by volume part proportioning is as follows for compound volatile oil and substrate: compound volatile oil 10%, substrate 90%.
In 21. claim 1-20, arbitrary described preparation is for making the purposes of sterilization with fresh air.
22. according to the purposes of claim 21, and it is dispersed in air, thus filtration, rinse air and reach the object that makes sterilization with fresh air.
23. want 21 or 22 purposes according to right, and described preparation is to be dispersed in air by heating and/or spray pattern.
The preparation method of preparation described in any one in 24. claim 1-20:
Described method comprises the step of extracting in described compound volatile oil component described in each.
25. preparation methoies according to claim 24:
Described method comprises in the described compound volatile oil of following extraction the step of component described in each:
Adopt steam distillation from the raw material of each component, to extract each component, and/or adopt supercritical fluid extraction from the raw material of each component, to extract each component.
26. according to the preparation method described in claim 24 or 25, and described method also further comprises:
Measure respectively each extracted component;
Mixed, optionally stirred evenly, to obtain described compound volatile oil.
27. according to the preparation method of claim 26, and described method is further comprising the steps of:
Measure described substrate, the substrate optionally multicomponent being formed is mixed, more optionally stirs evenly;
And described substrate is added the mixture of described each volatile oil to mix, optionally the mixture of volatile oil and substrate is stirred evenly, and obtain described disinfectant preparation with fresh air.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310283575.4A CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
| CN200710162111.2A CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
| HK14105895.9A HK1192416A1 (en) | 2007-12-14 | 2014-06-20 | Air fresh disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710162111.2A CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310283575.4A Division CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101455850A CN101455850A (en) | 2009-06-17 |
| CN101455850B true CN101455850B (en) | 2014-02-12 |
Family
ID=40767149
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310283575.4A Active CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
| CN200710162111.2A Active CN101455850B (en) | 2007-12-14 | 2007-12-14 | Air disinfectant |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310283575.4A Active CN103461394B (en) | 2007-12-14 | 2007-12-14 | Air refreshing disinfectant |
Country Status (2)
| Country | Link |
|---|---|
| CN (2) | CN103461394B (en) |
| HK (1) | HK1192416A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461394A (en) * | 2007-12-14 | 2013-12-25 | 永正顾问有限公司 | Air refreshing disinfectant |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010094151A1 (en) * | 2009-02-20 | 2010-08-26 | 永正顾问有限公司 | Disinfectant making air fresh and preparation method and use thereof |
| CN103191183A (en) * | 2012-07-30 | 2013-07-10 | 成都中医药大学 | Use of palchouli oil in preparation of antiviral drug |
| CN103891738A (en) * | 2012-12-31 | 2014-07-02 | 青岛锦涟鑫商贸有限公司 | Indoor air freshening disinfectant |
| CN104996503B (en) * | 2015-07-22 | 2017-09-29 | 四川农业大学 | It is a kind of for Chinese medicine compound prescription air sterillization spray of plant and preparation method thereof |
| CN105412965A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Sandalwood air freshener and preparation method thereof |
| CN105477662A (en) * | 2015-11-30 | 2016-04-13 | 陶超杰 | Jojoba oil air freshener and preparation method thereof |
| CN105412966A (en) * | 2015-11-30 | 2016-03-23 | 陶超杰 | Chinese herb air freshener and preparation method thereof |
| CN105561363A (en) * | 2016-01-21 | 2016-05-11 | 广西大学 | Natural antibacterial air freshener and preparation method thereof |
| CN106943821A (en) * | 2016-10-12 | 2017-07-14 | 贾明忠 | A kind of residential block nebulization dust system and its sprinkling solution additive |
| CN107343865B (en) * | 2017-08-03 | 2020-09-01 | 成都煜泉绿健科技有限公司 | Compound volatile oil and preparation method and application thereof |
| CN108721671A (en) * | 2018-06-13 | 2018-11-02 | 李帛昀 | Student's air freshener with functions of refreshing brain and restoring consciousness and preparation method thereof |
| CN108605999A (en) * | 2018-06-25 | 2018-10-02 | 杭州光启医疗科技发展有限公司 | A kind of medical bactericidal liquid and preparation method thereof |
| CN111109302B (en) * | 2020-02-22 | 2021-04-06 | 深圳超微颗粒产业有限责任公司 | Antimicrobial aerosol and toxicant exposure spray suspended particle spray and preparation method thereof |
| CN111493103A (en) * | 2020-05-21 | 2020-08-07 | 泓麟生物科技(广州)有限公司 | Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application |
| CN112205430B (en) * | 2020-09-07 | 2022-01-18 | 恩民医药科技有限公司 | Traditional Chinese medicine extract composition and preparation method and application thereof |
| CN112021695A (en) * | 2020-09-08 | 2020-12-04 | 东莞市华佑医疗科技有限公司 | Medical mask of compound Chinese medicinal aromatherapy essential oil for resisting virus and influenza |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1128672A (en) * | 1995-02-11 | 1996-08-14 | 卞伟 | Agent for purifying and refreshing air |
| CN1156540A (en) * | 1996-09-24 | 1997-08-13 | 南召县供销合作社联合社 | Air pure and fresh disinfectant using folium artemisiae argyi oil as main and preparing process thereof |
| CN1440660A (en) * | 2003-03-27 | 2003-09-10 | 李汉文 | Mouldproof and bacteriostatic aromatic oil product series and its production process |
| CN1742752A (en) * | 2005-10-08 | 2006-03-08 | 晏四平 | Atomizing agent containing medicinal plant oil |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321700C (en) * | 2004-12-30 | 2007-06-20 | 浙江理工大学 | Air disinfecting fumigant and its using method |
| CN103461394B (en) * | 2007-12-14 | 2015-07-22 | 永正顾问有限公司 | Air refreshing disinfectant |
-
2007
- 2007-12-14 CN CN201310283575.4A patent/CN103461394B/en active Active
- 2007-12-14 CN CN200710162111.2A patent/CN101455850B/en active Active
-
2014
- 2014-06-20 HK HK14105895.9A patent/HK1192416A1/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1128672A (en) * | 1995-02-11 | 1996-08-14 | 卞伟 | Agent for purifying and refreshing air |
| CN1156540A (en) * | 1996-09-24 | 1997-08-13 | 南召县供销合作社联合社 | Air pure and fresh disinfectant using folium artemisiae argyi oil as main and preparing process thereof |
| CN1440660A (en) * | 2003-03-27 | 2003-09-10 | 李汉文 | Mouldproof and bacteriostatic aromatic oil product series and its production process |
| CN1742752A (en) * | 2005-10-08 | 2006-03-08 | 晏四平 | Atomizing agent containing medicinal plant oil |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461394A (en) * | 2007-12-14 | 2013-12-25 | 永正顾问有限公司 | Air refreshing disinfectant |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103461394A (en) | 2013-12-25 |
| CN103461394B (en) | 2015-07-22 |
| CN101455850A (en) | 2009-06-17 |
| HK1192416A1 (en) | 2014-08-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101455850B (en) | Air disinfectant | |
| CN101502273B (en) | Chinese medicinal incense | |
| CN101884801B (en) | Air freshener and preparation method and application thereof | |
| CN102366389B (en) | Preparation method for compound mosquito repelling and itching relieving floral water | |
| CN102845485B (en) | Chinese medicinal extract disinfectant | |
| CN104336078A (en) | Chlorine-containing Chinese herbal medicine air disinfectant, and preparation method and application thereof | |
| CN102246840B (en) | Ampholytic organic pesticide | |
| CN111493103A (en) | Traditional Chinese medicine composition, traditional Chinese medicine air freshening disinfectant and application | |
| US8551541B2 (en) | Disinfectant making air fresh and preparation and method and use thereof | |
| CN103977155A (en) | Cleaning-free compound hand sanitizer and preparation method thereof | |
| CN110215532A (en) | A kind of plants essential oil water solvent combinations object and preparation method thereof | |
| CN106538629A (en) | A kind of livestock-raising disinfectant and preparation method thereof | |
| CN106265168A (en) | A kind of preparation method of natural composite plant mosquito-driving liquid antibacterial, antipruritic | |
| CN111685135A (en) | Traditional Chinese medicine plant extract with disinfection effect, preparation method and disinfectant | |
| CN102349548A (en) | Environment biological disinfectant with phages as main components and application thereof | |
| WO2022068977A2 (en) | Antimicrobial essential oil composition, product comprising same, and preparation method for product thereof | |
| CN108273106A (en) | A kind of anti-sense medicine is fragrant | |
| CN101843914B (en) | Composition with air disinfection function | |
| CN107343865B (en) | Compound volatile oil and preparation method and application thereof | |
| CN110063908A (en) | Nano emulsion type mouthwash and its preparation method and application method | |
| CN115068397B (en) | Antibacterial and anti-inflammatory toothpaste containing bamboo leaf flavone and preparation method thereof | |
| CN108355159A (en) | A kind of novel air freshener and its application | |
| CN105284930A (en) | Insect dispelling and bacterium inhibiting type air freshener prepared from ripe fruits of camphor trees and preparing method of air freshener | |
| Siddartha et al. | FORMULATION AND EVALUTION OF HERBAL NEEM SANITIZER | |
| CN106508897A (en) | Plant source sterilization aerosol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: Room 4, building 402, Wing Tai Arts crafts emporium, 106 Ming Ming Street, Kwun Tong, Kowloon, Hongkong, China Patentee after: Yong Zheng Consultant Co., Ltd. Address before: Room 603, Fu Plaza, 111 Qiao Ming Street, Kowloon, Hongkong, China Patentee before: Yong Zheng Consultant Co., Ltd. |