CN115251093A - Disinfecting composition containing aromatic Chinese medicinal essential oil and guarana extract and preparation method thereof - Google Patents

Disinfecting composition containing aromatic Chinese medicinal essential oil and guarana extract and preparation method thereof Download PDF

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CN115251093A
CN115251093A CN202210876909.8A CN202210876909A CN115251093A CN 115251093 A CN115251093 A CN 115251093A CN 202210876909 A CN202210876909 A CN 202210876909A CN 115251093 A CN115251093 A CN 115251093A
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essential oil
aromatic
guarana
chinese medicine
extract
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CN115251093B (en
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陈丽华
朱卫丰
陈婷
杨明
管咏梅
黄诗雨
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Jiangxi University of Traditional Chinese Medicine
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Jiangxi University of Traditional Chinese Medicine
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Abstract

The invention belongs to the technical field of disinfectants, and discloses a disinfection composition containing aromatic traditional Chinese medicine essential oil and guarana extract, which comprises the aromatic traditional Chinese medicine essential oil and the guarana extract; the mass ratio of the aromatic traditional Chinese medicine essential oil to the guarana extract is 3-5:5-15; the aromatic Chinese medicinal essential oil comprises folium Artemisiae Argyi, herba Agastaches, herba Eupatorii, herba Houttuyniae, flos Caryophylli, and cortex Cinnamomi. Also discloses a disinfectant containing the aromatic Chinese medicinal essential oil and the guarana extract, which is prepared into composite nano-emulsion, hand sanitizer and disinfection spray on the basis of the aromatic Chinese medicinal essential oil and the guarana extract. The traditional Chinese medicine disinfectant prepared by the aromatic traditional Chinese medicine essential oil and the guarana alcohol extract synergistically has the advantages of remarkable bacteriostatic effect and strong antibacterial effect.

Description

Disinfectant composition containing aromatic Chinese medicinal essential oil and guarana extract and preparation method thereof
Technical Field
The invention belongs to the technical field of disinfectants, and particularly relates to a disinfection composition containing aromatic traditional Chinese medicine essential oil and guarana extract and a preparation method thereof.
Background
Some microbes causing respiratory tract infection in the air can suspend and live for a long time, which is one of main vectors for disease transmission, and the reduction of the infection caused by air pathogenic bacteria is an important sanitary problem which needs to be solved urgently in public places. The currently used air disinfection methods include an ultraviolet irradiation method and a chemical disinfection method, both of which have certain sterilization efficacy, but still have limitations. The ultraviolet ray can only disinfect the surface of an object directly irradiated, and has the defects of incomplete sterilization, killing effect on cells and short time-lapse; chemical disinfectants such as high-efficiency peroxide peroxyacetic acid and strong oxidizing chlorine-containing disinfectants have strong bactericidal capacity, can kill bacterial propagules, fungi, viruses, mycobacteria, bacterial spores and the like, but have strong irritation; the phenolic disinfectant can quickly kill cells under high concentration, is a common environmental disinfectant in daily life, has toxic components, is only suitable for environments and objects, cannot be used for environmental sterilization of personnel in the field, and is limited in use. At present, the traditional air disinfection method with toxic and side effects is gradually eliminated, and natural disinfection products which are green and natural, high in safety coefficient and strong in sterilization effect are more and more valued by scientific researchers.
Throughout ancient times and modern times, chinese herbal medicines have a long history of being applied to epidemic diseases. The traditional Chinese medicine air disinfection method is to act on the skin and respiratory system of a human body to disinfect through aromatic medicines in a natural volatilization or combustion mode. The concept of traditional Chinese medicine air disinfection is not proposed in ancient China, but the application of traditional Chinese medicine in disinfection and killing clinic is successful. The ancient books put forward the traditional Chinese medicine air disinfection methods at the earliest, such as Taiyi Dingjin and Jing Wei Dan, which are mostly 'deep-red sacs and carried' or 'hung on the door'. After the ancient and modern epidemic prevention sachets are subjected to summary analysis, a large amount of aromatic medicines are used as main components, and the effect of eliminating turbid pathogen by means of aromatic gas is achieved. In the modern common sachet prescription, folium artemisiae argyi, eupatorium fortunei, rhizoma atractylodis and agastache rugosus are all high-frequency used medicines. The pharmacological research result of the sachet medicine shows that the active ingredients of the sachet medicine are mainly volatile oil, and the active ingredients are proved to have stronger antibacterial and antiviral effects, namely the theory of traditional Chinese medicine that the fragrant gas can drive pathogenic factors and avoid dirt is satisfied.
A modern traditional Chinese medicine air disinfection method is developed from the clinical application of the epidemic prevention sachet, and the plant essential oil capable of effectively sterilizing is selected to prepare the air disinfectant. Modern researches find that volatile oil such as folium artemisiae argyi and pogostemon cablin has good antibacterial and antiviral effects and has the advantages of high safety, wide bactericidal spectrum and the like, so that a green and safe disinfectant is widely favored by the market, the advantages of traditional Chinese medicines are fully exerted in the development of disinfectant products, and the sterilizing effect of the traditional Chinese medicines is maximized.
Disclosure of Invention
The invention is provided for solving the defects in the prior art, and the first aim is to provide a disinfection composition containing aromatic traditional Chinese medicine essential oil and guarana extract, so as to solve the problems of environment and irritation in the prior disinfection technology;
the second purpose is to provide a preparation method of the disinfectant containing the aromatic traditional Chinese medicine essential oil and the guarana extract, the preparation method is simple in process, convenient to operate and suitable for large-scale industrial production;
the third purpose is to provide the application of a plurality of disinfection compositions containing aromatic traditional Chinese medicine essential oil and guarana extract, the application provides different types of products for the disinfection field, and the requirements of different disinfection approaches are met.
The invention provides a disinfection composition containing aromatic traditional Chinese medicine essential oil and guarana extract, which comprises the aromatic traditional Chinese medicine essential oil and the guarana extract; the mass ratio of the aromatic traditional Chinese medicine essential oil to the guarana extract is 3-5:5-15; the aromatic Chinese medicinal essential oil comprises folium Artemisiae Argyi, herba Agastaches, herba Eupatorii, herba Houttuyniae, flos Caryophylli, and cortex Cinnamomi.
A disinfectant containing aromatic Chinese medicinal essential oil and guarana extract is prepared into compound nanoemulsion, hand sanitizer and disinfection spray based on the aromatic Chinese medicinal essential oil and the guarana extract.
A composite nanoemulsion containing aromatic traditional Chinese medicine essential oil and guarana extract comprises the following components in percentage by weight: 5-15% of guarana extract, 3-5% of aromatic traditional Chinese medicine essential oil, a surfactant and a cosurfactant, wherein the aromatic traditional Chinese medicine essential oil: surfactant (b): the ratio of the cosurfactant is 1:4:5, and the balance of water.
The optimal compatibility proportion of the aromatic Chinese medicinal essential oil is that the essential oil of the Chinese mugwort leaves: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3: and 4, mixing according to the volume ratio.
Further, the surfactant is polyoxyethylene hydrogenated castor oil (RH 40), polysorbate-80 (Tween-80), caprylic capric polyethylene glycol glyceride (Labrasol), preferably polyoxyethylene hydrogenated castor oil; the cosurfactant is absolute ethyl alcohol, 1, 2-propylene glycol, 1, 3-butanediol and n-butyl alcohol, and is preferably absolute ethyl alcohol; the water is purified water.
Further, the guarana extract is a guarana alcohol extract; weighing 50g of guarana seed coarse powder, adding 10 times of 70% ethanol solution, extracting for 1h under reflux twice, filtering the two extracting solutions, collecting and combining the two extracting solutions, and concentrating in a rotary evaporator until the relative density is more than 1.0.
The invention provides a preparation method of a composite nano-emulsion containing aromatic traditional Chinese medicine essential oil and guarana extract, which comprises the following steps:
(1) Mixing surfactant polyoxyethylene hydrogenated castor oil, co-surfactant anhydrous alcohol and aromatic Chinese medicinal essential oil at a certain proportion, stirring for 10min to mix well;
(2) Adding the alcohol extract of guarana, and stirring for 10min until the mixture is clear and transparent;
(3) Adding purified water with the prescription amount into the obtained product, and continuously stirring for 20min to obtain the compound essential oil guarana composite nanoemulsion with clear color.
The stirring speed is 600rpm/min.
The application of the disinfection composition containing the aromatic Chinese medicinal essential oil and the guarana extract comprises the application of disinfection on kitchen boards, hand sterilization and disinfection, sterilization cream, sterilization gel and the like.
Compared with the prior disinfection technology, the disinfection composition containing the aromatic traditional Chinese medicine essential oil and the guarana extract has the beneficial effects that: the composition has the effects of remarkable antibacterial effect, strong antibacterial effect and good application prospect. The pure aromatic Chinese medicinal essential oil disinfectant can completely kill common pathogenic bacteria within 30s and dominant putrefying bacteria within 3 h; compared with the disinfection effect of the pure essential oil disinfectant, the compound aromatic Chinese medicine essential oil compatible guarana air disinfectant has the effect of 100% inactivation on common pathogenic bacteria in the air within 30s and completely killing dominant putrefying bacteria within 30min, and has the inhibition effect on the pathogenic bacteria within 15s better than that of the pure essential oil disinfectant, so that the disinfection effect is quick and strong, and the compound aromatic Chinese medicine essential oil compatible guarana air disinfectant is a green strong disinfection product. The materials are natural, nontoxic, high in safety factor, capable of being used in human environment and free of residue after being acted. Wherein, the alcohol content is below 30%, effectively prevents the disinfectant from mildewing, is easy to transport, and avoids accidents such as explosion caused by overhigh alcohol concentration of the disinfectant. In addition, the multiple applications provided by the invention provide products with the functions of sterilization, bacteriostasis and drug resistance for different disinfection channels, and meet the requirements of different industries.
The technical scheme of the invention has the following functions of various aromatic traditional Chinese medicines:
folium artemisiae argyi: the dry leaves of the Artemisia argyi of the Compositae family are bitter, warm and pungent in nature, have the functions of expelling cold and dampness, warming channels, regulating qi and blood, stopping bleeding, preventing miscarriage and the like, and have various clinical effects of antibiosis, anti-inflammation, antioxidation and the like. The main chemical components of the composition comprise volatile oil, flavonoid, tannin, triterpenes, eudesmane, polysaccharide, trace elements and the like. Researches show that the folium artemisiae argyi has the effect of preventing diseases and plagues, and has obvious inhibition effects on tubercle bacillus, bacillus subtilis, escherichia coli, staphylococcus aureus and the like. Modern researches have considered that the folium artemisiae argyi has certain analgesic effect besides inhibiting or killing various fungi, bacteria and viruses.
Rhizoma atractylodis: dried rhizome of Atractylodes lancea or Atractylodes lancea of Compositae. Is pungent and bitter in flavor, and has the effects of eliminating dampness, strengthening spleen, dispelling wind and cold and improving eyesight. Research shows that the main components of the rhizoma atractylodis include volatile oil components such as atractylone, paleoxol and beta-cineol, and the rhizoma atractylodis has an inhibiting effect on the growth of pathogenic bacteria such as staphylococcus aureus, escherichia coli, candida albicans, trichophyton rubrum and the like. Rhizoma Atractylodis can destroy organelles, change cell structure, and kill bacteria. Experimental data show that the rhizoma atractylodis volatile oil spray, fumigation liquid and fumigant can inhibit bacteria such as staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and the like in the air. Meanwhile, the rhizoma atractylodis can also play a role in killing influenza viruses by inhibiting virus DNA.
Patchouli: the dry aerial parts of Pogostemon cablin of Labiatae, pungent in flavor and slightly warm in nature, have the effects of aromatizing, resolving dampness, regulating the middle warmer, relieving vomit, relieving exterior syndrome and relieving summer-heat. The main components of the patchouli are volatile oil: patchouli alcohol, alpha-patchouli alkene, beta-patchouli alkene, and the like. Modern researches show that the product has the effects of resisting bacteria, fungi, plasmodium and plant pathogenic fungi, regulating gastrointestinal motility, promoting secretion of digestive juice, protecting intestinal barrier function, regulating immunity, etc. It has broad-spectrum antibacterial activity, and has effects of inhibiting and even killing various bacteria and fungi, and has certain inhibiting ability on Escherichia coli, bacillus subtilis, staphylococcus albus, and Staphylococcus aureus.
Herba Eupatorii: the Eupatorium fortunei of Compositae has dry aerial parts, pungent taste, and effects of eliminating dampness with aromatics, activating spleen, stimulating appetite, relieving exterior syndrome, and relieving summer-heat. Pharmacological research shows that the medicine has the functions of resisting inflammation, inhibiting bacteria, resisting tumor, eliminating phlegm, resisting tumor, strengthening immunity, exciting stomach smooth muscle, etc. The traditional Chinese medicine composition is clinically used for treating coronary heart disease, summer-heat and dampness cold, infant rotavirus enteritis, infant diarrhea and the like. The eupatorium contains essential oil: para-cymene, aurantiol acetate, thymol methyl ether, etc. Research shows that the water decoction has the effect of inhibiting diphtheria, staphylococcus aureus, sarcina, proteus and typhoid bacillus. The volatile oil and monomer components including cymene and neryl acetate have direct inhibitory effect on influenza virus.
Clove: the dried flower bud of Eugenia caryophyllata of Myrtaceae has pungent and warm natured taste, and has effects of warming spleen and stomach, lowering adverse qi, dispelling cold, relieving pain, warming kidney and tonifying yang. Modern research shows that the essential oil mainly contains the following volatile oil: eugenol, acetyl eugenol, beta-caryophyllene, methyl n-amyl ketone, methyl salicylate, etc., wherein eugenol is one of the effective components in clove volatile oil, and the decoction has the inhibiting effect on staphylococcus, streptococcus and bacilli such as diphtheria, deformity, pyocyanea, large intestine, etc. The clove bacteriostasis mechanism is that the permeability of bacteria is increased, the thallus morphology is abnormal, cell structures such as cell walls, cell membranes and the like are damaged, a large amount of cell contents are leaked out, even cell malformation occurs, and the clove bacteriostasis mechanism has the inhibiting effect on bacteria such as escherichia coli, staphylococcus aureus, candida albicans, helicobacter pylori, escherichia coli and the like and viruses.
Heartleaf houttuynia herb: fresh whole plant or dried aerial parts of houttuynia cordata of Saururaceae, is pungent and slightly cold in nature, and has effects of clearing heat and detoxicating, resolving carbuncle and expelling pus, and inducing diuresis for treating stranguria. Modern researches show that the cordate houttuynia mainly contains volatile oil, and the houttuynine sodium bisulfite contained in the volatile oil has different degrees of inhibition effects on various gram positive and negative bacteria such as staphylococcus aureus, diplococcus pneumoniae, streptococcus a, influenza bacillus and the like.
Cinnamon: the dry bark of cinnamon belonging to Lauraceae family is hot, pungent and sweet in flavor. Modern researches show that the main components of the traditional Chinese medicine composition are cinnamyl aldehyde, salicylaldehyde and the like, and the traditional Chinese medicine composition has the effects of dispelling wind and invigorating stomach, promoting blood circulation to remove blood stasis, dispelling cold and relieving pain, diminishing inflammation and sterilizing and the like.
Melon and sodium: also known as balsamine, belongs to the family of soapberry, is produced in the tropical plants of the brazilian amazonian basin, is used in the fields of medical care, food industry, cosmetics and the like, and has a wide range of consumer groups. Mainly contains chemical components such as alkaloid, polyphenol, tannic acid, etc., and the main medicinal parts are fruits and seeds. Modern researches show that the guarana has antioxidation and antibacterial effects, and has certain effects in resisting fatigue, improving memory, resisting depression, resisting tumor, etc.
Drawings
FIG. 1 is a diameter chart of inhibition zones of 7 essential oils.
Fig. 2 is a dynamic bacteriostatic curve.
Detailed Description
The present invention is further explained with reference to the following specific embodiments in order to make the objects and advantages of the present invention more comprehensible, but the present invention is not limited to the specific embodiments, and those skilled in the art may similarly extend without departing from the spirit and scope of the present invention.
Example 1
According to the mass percentage, 3% of aromatic Chinese medicine essential oil is taken in a container, and the aromatic Chinese medicine essential oil is the artemisia leaf essential oil: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight being 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight being 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min to mix evenly. Adding water to make up the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil air spray disinfectant.
Example 2
According to the mass percentage, 3% of aromatic Chinese medicine essential oil is taken in a container, and the aromatic Chinese medicine essential oil is the artemisia leaf essential oil: patchouli essential oil: herba Eupatorii essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min for uniform mixing. Adding 5% alcohol extract of guarana, and magnetically stirring at 600rpm for 10 min. Adding water to supplement the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil guarana air spray disinfectant.
Example 3
According to the mass percentage, 3% of aromatic Chinese medicine essential oil is taken in a container, and the aromatic Chinese medicine essential oil is the artemisia leaf essential oil: patchouli essential oil: herba Eupatorii essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight being 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight being 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min to mix evenly. Then adding the 15 percent of the guarana alcohol extract, and stirring for 10min by magnetic force at 600rpm to mix evenly. Adding water to supplement the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil guarana air spray disinfectant.
Example 4
Taking 4% of aromatic Chinese medicinal essential oil in a container according to the mass percentage, wherein the aromatic Chinese medicinal essential oil is the folium artemisiae argyi essential oil: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min for uniform mixing. Adding water to make up the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil air spray disinfectant.
Example 5
According to the mass percentage, 5 percent of aromatic Chinese medicinal essential oil is taken into a container, and the aromatic Chinese medicinal essential oil is the artemisia leaf essential oil: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight being 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight being 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min to mix evenly. Adding water to make up the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil air spray disinfectant.
Example 6
According to the mass percentage, 5 percent of aromatic Chinese medicinal essential oil is taken into a container, and the aromatic Chinese medicinal essential oil is the artemisia leaf essential oil: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight being 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight being 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min to mix evenly. Adding the 5% alcohol extract of guarana, and magnetically stirring at 600rpm for 10 min. Adding water to supplement the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil guarana air spray disinfectant.
Example 7
According to the mass percentage, 5% of aromatic Chinese medicine essential oil is taken in a container, and the aromatic Chinese medicine essential oil is the artemisia leaf essential oil: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, adding polyoxyethylene hydrogenated castor oil with the weight being 4 times that of the aromatic Chinese medicinal essential oil, adding cosurfactant anhydrous ethanol with the weight being 5 times that of the aromatic Chinese medicinal essential oil, and magnetically stirring at 600rpm for 10min to mix evenly. Adding the 15% alcohol extract of guarana, and magnetically stirring at 600rpm for 10 min. Adding water to supplement the balance, magnetically stirring at 600rpm for 20min, mixing, sterilizing, and bottling to obtain the compound essential oil guarana air spray disinfectant.
The following are toxicity evaluation, irritation and sterilization experiments performed on the disinfectants prepared in the above examples to illustrate the effects of the present invention:
1. acute toxicity test animals: 40 SPF-grade KM mice are purchased from the animal experiment center of the university of traditional Chinese medicine in Jiangxi, the male and female are half, and the weight is 18-22g.
The experimental method comprises the following steps: one-time maximum test is carried out according to the specification of 2002 edition 'disinfection technical specification', for example, the one-time gavage dosage of 5000mg/kg & BW for 20 animals (each half of a male and a female), no death is caused in 14 days, and LD can be judged50Greater than 5000mg/kg body weight. The evaluation rules are: LD (laser diode)50The people with the weight more than 5000mg/kg are actually nontoxic and LD50The patients with more than 501mg/kg-5000mg/kg body weight belong to low toxicity and LD50The body weight of 51mg/kg-500mg/kg belongs to the medium toxicity and LD501mg/kg-50 mg/kg of body weight belongs to high toxicity and LD50Those with the weight less than 1mg/kg are extremely toxic.
The experimental steps are as follows: the test was randomized into 3 test groups (examples 1, 5, 7) and 1 blank control group, and fasted overnight prior to testing using a one-time maximal test. The gavage dose of the test group was 6000mg/kg BW, and the same amount of sterile water was given to the control group. After gavage, the animals were observed for signs of intoxication and number of deaths and time of death, recorded for 14d, and dead animals and animals after the observation were necropsied and histopathologically examined.
As a result: no death occurred in 14d, and no other abnormal conditions existed. According to the classification standard of acute toxicity of the disinfectant in 2002 edition of disinfection technical Specification of the Ministry of public health of the people's republic of China, the tested samples 1, 5 and 7 are all practically nontoxic.
2. Skin and mucosa irritation test the irritation/corrosion effect and strength of the disinfectant on the skin of the test animals. Experimental animals: healthy rabbits or guinea pigs with intact skin are purchased from the animal experiment center of the university of traditional Chinese medicine in Jiangxi.
The experimental steps are as follows: referring to the disinfection technical Specification, 2002 edition, ministry of health of the people's republic of China, new Zealand rabbits were used for a complete skin irritation test and a skin breakage test, respectively, and for an acute eye irritation test. At 24h before the test, hairs on both sides of the spine of the rabbit or guinea pig are removed by depilatory, the hair removal range is about 3cm × 3cm on the left and right, respectively, and the skin is not damaged. The next day, the test substance (stock solution 0.5 ml) was directly dropped on the intact skin with hair removed on one side of 3cm × 3cm area, covered with a layer of gauze, and fixed with non-irritating adhesive tape. The depilated skin on the other side was used as a blank control and the application time was 4h. After the test is completed, the residual test substance is removed with warm water or a non-irritating solvent. Local skin reactions were observed at 1h, 24h and 48h after removal of the test substance, respectively, and stimulation responses were scored for performance. Each 3 new zealand rabbits were grouped. Setting an experimental group and a blank control group, wherein 0.5mL of stock solution is smeared on the experimental group of the skin irritation test, and 0.1mL of stock solution is dripped on the experimental group of the acute eye irritation test.
As a result: in a one-time medicine application complete skin experiment, local skin reactions of New Zealand rabbits for 1 hour, 24 hours and 48 hours are observed, and no erythema or edema phenomenon is found; in a skin damage experiment of a New Zealand rabbit, the back wound of the New Zealand rabbit is found to be normally scabbed, and the wound recovery is not influenced; eye mucosa stimulation is carried out on New Zealand rabbits, liquid medicines are respectively dripped, and observation is carried out after 1h, 24h, 48h, 72h and 7d of eye dripping, and the observation shows that the experimental group is normal in the 1h period except for the blood streak of eyes of individual rabbits. It can thus be determined that: the disinfectants prepared in the examples 1, 5 and 7 of the present invention have no irritation to the skin and mucous membranes.
The effect of the invention is further illustrated by combining the experiment of the bacteriostatic rate of the disinfectant:
7 antibacterial zone test experiment of essential oil
Pouring agar cooled to 40 ℃ into the plate, standing and condensing for later use. Formulation 1X105Sucking 200ul of the strain suspension (see Table 1 for specific strains), putting into a plate, coating with a coating rod, standing for 5min, and naturally drying at room temperature. 10ul of essential oil is dripped into each filter paper sheet, and the dripped surface is uniformly placed on a bacteria-containing plate with 3 sheets per plate. And (4) carrying out inverted culture at 37 ℃ for 16-18h, observing the result, and measuring the diameter of the inhibition zone. The test results are shown in table 1.
TABLE 1 bacteriostatic circle diameter of 7 essential oils
Figure BDA0003762731280000051
Note: the judgment standard of the sensitivity degree of the medicine is hypersensitive (S + +) above 20; 15-20 hypersensitive (S +); 10-14 mesomine (S); hyposensitization (S-);
as can be seen from the in vitro antibacterial data in Table 1 and FIG. 1, the volatile oils have good antibacterial effect, wherein the clove has the strongest antibacterial ability, has good killing effect on Escherichia coli and Staphylococcus aureus, has the same antibacterial effect as the positive drug gentamicin, has the diameter of the antibacterial zone of more than 20, and shows high sensitivity to bacteria. Therefore, through orthogonal design experiments, the essential oil is mixed in proportion, and test results show that the folium artemisiae argyi essential oil can be obtained: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3:4, mixing according to the volume ratio, and providing prescription data support for the preparation of the subsequent Chinese herbal compound essential oil melon radon antibacterial spray, wherein the mixture is a better antibacterial compatibility combination.
3. Disinfection Effect test
The sterilization effect of the traditional Chinese medicine disinfectant on selected pathogenic bacteria is tested by adopting the standard WS/T650-2019 in the above embodiments 1,3, 5 and 7, the stock solution of the disinfectant is used for actual measurement according to the standard so as to ensure the consistency of the concentrations of the samples, and the sterilization effect of each embodiment is compared and analyzed.
The bacterial suspension prepared in advance was taken and diluted to about 5.0X 10 with PBS5CFU/mL～4.5×106CFU/mL bacterial suspension is ready for use. Taking a sterile test tube, adding 5.0mL of sample (using stock solution), placing the sterile test tube in a water bath kettle at the temperature of 20 +/-1 ℃ for 5min, then adding 0.1mL of test bacterial suspension, quickly mixing uniformly and immediately timing. After the test bacteria and the sample interact for 15s, 0.5min, 1.5min, 2.5min and 5min, respectively sucking 1.0mL of test bacteria and sample mixed solution for inoculation2 plates, and pouring the culture medium (when the bacterial count can not be counted, performing 10-fold serial dilution by PBS, selecting appropriate dilution, respectively sucking 1.0mL to inoculate 2 plates, and performing live bacterial culture counting). At the same time, PBS was used in place of the sample for parallel testing as a positive control. The number of colonies recovered from the positive control was 1.0X 104CFU/mL～9.0×104CFU/mL. And (5) inoculating the PBS and the culture medium of the same batch into a culture dish. All test samples and control samples are cultured at 36 +/-1 ℃, and bacterial propagules are cultured for 48h to observe the final result; the Candida albicans is cultured for 72h to observe the final result. The test is repeated for 3 times, and the bacteriostasis rate is calculated.
The results of the 15s bacteriostasis test of examples 1,3, 5 and 7 on 4 bacteria are as follows:
TABLE 2 results of 15s bacteriostasis for each example
Figure BDA0003762731280000052
According to the in vitro bacteriostasis rate test experiment result, the aromatic Chinese medicinal essential oil air spray disinfectant and the aromatic Chinese medicinal essential oil compatible guarana air disinfectant prepared by the method have excellent bactericidal effects, and have obvious killing effects on common pathogenic bacteria white staphylococcus in the air, common pathogenic bacteria escherichia coli and staphylococcus aureus on the surface of an object. The disinfectant stock solutions and the bacterial suspensions in the embodiments 1,3, 5 and 7 are respectively acted for different times of 0.5min, 1.5min, 2.5min and 5min, and the results show that all pathogenic bacteria can be killed within 0.5min, which indicates that the compound essential oil and the compound essential oil compatible guarana alcohol extract both have extremely excellent bactericidal action. On the basis of the research, the disinfection effect of the 4 examples on several common pathogenic bacteria is researched by shortening the time (15 s) and diluting the stock solutions (the dilution times of examples 1 and 3 are 2; and the dilution times of examples 5 and 7 are 5), the results are shown in table 2, after the disinfectants are diluted, the disinfectants still have strong inhibition effect on the 4 pathogenic bacteria, and the two concentrations of the essential oil compatible guarana extract disinfectants have higher bacteriostasis rate and better effect than pure essential oil disinfectants.
According to the invention, five common aromatic traditional Chinese medicines of folium artemisiae argyi, clove, patchouli, fortune eupatorium herb and cinnamon are preferably selected from ancient and modern sachets, and the heartleaf houttuynia herb essential oil and the guarana alcohol extract are optimally added on the basis of compatibility of the traditional Chinese medicines.
4. Test of Corrosion prevention Effect
Inhibiting effect of disinfectant on dominant putrefying bacteria
The disinfectant prepared in examples 5 and 6 and a normal saline control group are respectively subjected to determination of bacteriostatic efficacy test method of lactobacillus according to China pharmacopoeia 1121 of 2020 edition by using stock solution.
The experimental steps are as follows: preparing lactobacillus (Lactobacillus acidophilus) within 5 generations with sterile normal saline solution to obtain a solution containing 10 bacteria per 1ml8Bacterial suspension of cfu. The disinfectant prepared in the 2 examples and the control group are respectively absorbed by 5.0ml in a centrifugal tube, different bacterial strains are added into each tube, and the inoculation bacterial quantity in 1g or 1ml of test article is 105-106cfu, the volume of the inoculated bacterial liquid is not more than 1 percent of the volume of the test sample, and the inoculated bacterial liquid is fully mixed and then placed at 20 to 25 ℃ for dark storage. Then, the number of viable bacteria was measured, and 1ml of the sample was taken out from the centrifugal tube at intervals shown in the graph, and the number of bacteria contained in the sample was measured. MRS agar was poured. According to the determination result of the number of viable bacteria, the number of bacteria added by each test bacteria of 1ml of a test sample and the number of bacteria at each interval time are calculated and converted into lg value, the obtained result is shown in figure 2, the total number of the lactic acid bacteria in a control group is in an ascending trend within 8 hours, a traditional Chinese medicine compound essential oil disinfectant containing 5% of aromatic traditional Chinese medicine essential oil can kill a large amount of putrefying bacteria after 1 hour, and the lactic acid bacteria can be completely killed after 3 hours; the traditional Chinese medicine compound essential oil disinfectant containing 5% of aromatic traditional Chinese medicine essential oil and 5% of guarana alcohol extract can kill all putrefying bacteria within 1 hour, the guarana alcohol extract enhances the bacteriostatic effect of the disinfectant, and meanwhile, the guarana is commonly used for production research of daily drinks and health care products, has the bacteriostatic effect and is safe and controllable as an edible medicine-food homologous product, and is a great choice in the field of food preservation.
The invention adopts the common aromatic traditional Chinese medicine essential oil of ancient and modern sachets to be compatible with the guarana alcohol extract, and the guarana alcohol extract is uniformly mixed with the polyoxyethylene hydrogenated castor oil and the absolute ethyl alcohol to prepare the nano-emulsion disinfectant, and the effect of the traditional Chinese medicine compound essential oil disinfectant of different embodiments is evaluated through the sterilization effect on 4 different pathogenic bacteria of escherichia coli, staphylococcus aureus, staphylococcus albus and candida albicans. The experimental result shows that the disinfectant containing aromatic Chinese medicinal essential oil with the concentration of 3 percent or more has obvious bacteriostatic action. The cinnamon essential oil, the clove essential oil and the like have extremely strong antibacterial and anti-inflammatory effects, and the cinnamon essential oil, the clove essential oil and the like are used together with the essential oil of the folium artemisiae argyi, the patchouli and the like and the guarana, so that the synergistic effect is achieved for exerting the antibacterial effect of the disinfectant. The guarana is a medicine-food homologous plant, the fruit and the seed of the guarana can be eaten, and the effective rate of inhibiting putrefying bacteria is obviously superior to that of the original compound essential oil composition as shown by the experimental result of the aromatic Chinese medicinal essential oil and the guarana alcohol extract disinfectant.
The use method of the disinfectant comprises the following steps:
(1) And (3) household sanitation and disinfection: taking a proper amount of the traditional Chinese medicine compound essential oil disinfectant, and mixing the traditional Chinese medicine compound essential oil disinfectant with the water for sterilization, wherein the weight ratio of 1:1, spraying the diluted disinfectant on the surface of an object to ensure that the disinfectant is fully contacted with the surface of the object, and standing for several minutes. Is suitable for sterilizing objects such as kitchen chopping boards, garbage cans, floors, cutters, rags and the like.
(2) Personal hygiene and disinfection: taking the traditional Chinese medicine compound essential oil disinfectant, 1:1 it can be diluted and sprayed on hand skin for skin sterilization.
(3) Food preservation: soaking the food to be treated in the compound essential oil guarana nanoemulsion for treating for 0.5h, air-drying and then carrying out vacuum packaging.
It should be noted that the above examples are only used to illustrate the technical solution of the present invention for preparing the traditional Chinese medicine disinfectant by combining the aromatic traditional Chinese medicine essential oil and the guarana alcohol extract, but not limited thereto, and the method of the preparation ratio can also be used in traditional Chinese medicine disinfecting gel, traditional Chinese medicine disinfecting cream and traditional Chinese medicine disinfecting lotion.

Claims (10)

1. A disinfection composition containing aromatic traditional Chinese medicine essential oil and guarana extract is characterized in that: comprises aromatic Chinese medicinal essential oil and guarana extract; the mass ratio of the aromatic traditional Chinese medicine essential oil to the guarana extract is 3-5:5-15; the aromatic Chinese medicinal essential oil comprises folium Artemisiae Argyi, herba Agastaches, herba Eupatorii, herba Houttuyniae, flos Caryophylli, and cortex Cinnamomi.
2. A disinfectant containing aromatic Chinese medicinal essential oil and guarana extract is characterized in that: the compound nanoemulsion, the hand sanitizer and the disinfection spray are prepared on the basis of the aromatic traditional Chinese medicine essential oil and the guarana extract, wherein the mass ratio of the aromatic traditional Chinese medicine essential oil to the guarana extract is 3-5:5-15.
3. A composite nanoemulsion containing aromatic traditional Chinese medicine essential oil and guarana extract is characterized in that: the components and weight percentage are as follows: 5-15% of guarana extract, 3-5% of aromatic traditional Chinese medicine essential oil, a surfactant and a cosurfactant, wherein the aromatic traditional Chinese medicine essential oil: surfactant (b): the ratio of the cosurfactant is 1:4:5, and the balance of water.
4. The compound nanoemulsion containing the aromatic Chinese medicine essential oil and the guarana extract as claimed in claim 3, is characterized in that: the optimal compatibility proportion of the aromatic Chinese medicinal essential oil is that the essential oil of the Chinese mugwort leaves: patchouli essential oil: eupatorium essential oil: houttuynia essential oil: clove essential oil: cinnamon essential oil =3:1:1:3:3: and 4, mixing according to the volume ratio.
5. The compound nanoemulsion containing the aromatic Chinese medicinal essential oil and the guarana extract as claimed in claim 3, wherein the compound nanoemulsion is characterized in that: the surfactant is one of polyoxyethylene hydrogenated castor oil, polysorbate-80 and caprylic capric acid polyethylene glycol glyceride.
6. The compound nanoemulsion containing the aromatic Chinese medicine essential oil and the guarana extract as claimed in claim 3, is characterized in that: the cosurfactant is one of absolute ethyl alcohol, 1, 2-propylene glycol, 1, 3-butanediol and n-butyl alcohol.
7. The compound nanoemulsion containing the aromatic Chinese medicine essential oil and the guarana extract as claimed in claim 3, is characterized in that: the guarana extract is an alcohol extract of the guarana.
8. The compound nanoemulsion containing the aromatic Chinese medicinal essential oil and the guarana extract as claimed in claim 7, wherein the compound nanoemulsion is characterized in that: the preparation process of the guarana alcohol extract comprises the following steps: weighing 50g of guarana seed coarse powder, adding 10 times of 70% ethanol solution, extracting for 1h under reflux twice, filtering the two extracting solutions, collecting and combining the two extracting solutions, and concentrating in a rotary evaporator until the relative density is more than 1.0.
9. A method for preparing the compound nanoemulsion containing the aromatic Chinese medicinal essential oil and the guarana extract as claimed in any one of claims 3 to 8 comprises the following steps:
(1) Mixing surfactant polyoxyethylene hydrogenated castor oil, co-surfactant anhydrous alcohol and aromatic Chinese medicinal essential oil at a certain proportion, stirring for 10min to mix well;
(2) Adding alcohol extract of guarana, and stirring for 10min until the mixture is clear and transparent;
(3) Adding purified water with the prescription amount into the obtained product, and continuously stirring for 20min to obtain the compound essential oil guarana composite nanoemulsion with clear color.
10. The use of the disinfecting composition of claim 1 comprising an aromatic Chinese medicinal essential oil and guarana extract for disinfecting kitchen boards, disinfecting hands, and disinfecting creams and gels.
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ADRIANA BASIL: "Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart" *
沈南鹏: "瓜拉纳提取物的功能性研究进展" *

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